CN114044820A

CN114044820A - Secondary separation extraction equipment for antibody extraction

Info

Publication number: CN114044820A
Application number: CN202111567068.4A
Authority: CN
Inventors: 彭波; 檀绍晨
Original assignee: Hebei Kanglide Biotechnology Co ltd
Current assignee: Hebei Kanglide Biotechnology Co ltd
Priority date: 2021-12-20
Filing date: 2021-12-20
Publication date: 2022-02-15

Abstract

The invention relates to the technical field of antigen and antibody, in particular to secondary separation and extraction equipment for antibody extraction, which comprises a bottom plate, wherein the bottom plate is connected with a box body, the box body is provided with a first motor, the first motor is connected with a rotating shaft, the rotating shaft is connected with a plurality of groups of stirring blades, the box body is communicated with a feeding pipe, the box body is communicated with a connecting pipe, the connecting pipe is provided with a second motor, the second motor is connected with a packing auger, the connecting pipe is communicated with a fixed pipe, the fixed pipe is provided with a first discharge valve, the connecting pipe is connected with a wide-mouth pipe, the bottom plate is connected with a supporting plate, the supporting plate is connected with a first placing plate, the convex chute is in sliding fit with a convex slide block, the convex slide block is connected with a first containing box, the wide-mouth pipe is provided with a second discharge valve, the supporting plate at the right part is connected with a second placing plate, the second placing plate is connected with a second containing box, and residues with extracting solution with raw materials can be separated again, thereby can reduce extravagantly, the practicality is higher.

Description

Secondary separation extraction equipment for antibody extraction

Technical Field

The invention relates to the technical field of antigen and antibody, in particular to secondary separation and extraction equipment for antibody extraction.

Background

When the antigen and antibody are extracted, a separation and extraction device is needed, and through retrieval, the invention patent with Chinese patent application number CN201520704050.8 discloses an extraction and purification device for the antibody, which is roughly described as comprising a plate frame, a drainage tube and a multi-way valve, wherein the extraction and purification column comprises an extraction and purification column body, a crude resin separation column, an ion exchange column and a hydrophobic column, the crude resin separation column is communicated with a liquid inlet of the extraction and purification column body, and the ion exchange column is respectively communicated with the crude resin separation column and the hydrophobic column through pipelines. According to the extraction and purification device for the antibody, the absorbent cotton slivers are arranged between the extraction and purification columns in each group, so that cross contamination of the extraction and purification columns can be effectively prevented; through drainage tube and the UNICOM of multi-ported valve, can realize once only can inject the antibody and draw the purification in the purification post to the multiunit.

The purification process of antibody can be accomplished to foretell prior art scheme when using by high flux, improves purification efficiency greatly and satisfies the needs of biotechnology field development, and its purification process that can accomplish the antibody by high flux, but its residue and the extract to the raw materials of being not convenient for separate once more to cause certain waste easily, the practicality is lower.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides the separation and extraction device which is convenient for separating the residue and the extracting solution of the raw materials again, so that the waste can be reduced, and the practicability is higher.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a secondary separation and extraction device for antibody extraction comprises a bottom plate, wherein a box body is fixedly connected to the left part of the top of the bottom plate, a first motor is installed at the top end of the box body, a rotating shaft is fixedly connected to the output end of the first motor, the bottom end of the rotating shaft penetrates through the box body and extends into the box body, a plurality of groups of stirring blades are fixedly connected to the outer circumferential wall of the rotating shaft, an inlet pipe is communicated with the left part of the top end of the box body, a connecting pipe is communicated with the lower part of the right end of the box body, a second motor is installed at the right end of the connecting pipe, an auger is fixedly connected to the output end of the second motor, the left end of the auger penetrates through the connecting pipe and extends into the connecting pipe, a fixed pipe is communicated with the right part of the bottom end of the connecting pipe, a first discharge valve is installed in the middle part of the outer circumferential wall of the fixed pipe, a plurality of water outlet holes are formed in the bottom end of the connecting pipe, and a wide-mouth pipe is fixedly connected to the bottom end of the connecting pipe, the bottom of wide-mouthed pipe runs through the bottom plate and extends to the outside to the equal fixedly connected with backup pad in bottom left part and the right part of bottom plate, each the board is placed to the looks remote site fixedly connected with of backup pad first, protruding type spout has been seted up to the first top right part of placing the board, the inner wall sliding fit of protruding type spout has protruding type slider, the first case that holds of top fixedly connected with of protruding type slider to the circumference outer wall lower part of wide-mouthed pipe is installed the second and is arranged the material valve, and the board is placed to the right-hand member fixedly connected with second that is located the backup pad of right part, the top fixedly connected with second that the board was placed to the second holds the case.

Preferably, a reinforcing rod is fixedly connected to the right end of the supporting plate at the right part, and the top end of the reinforcing rod is fixedly connected with the bottom end of the second placing plate.

Preferably, the left end of the inner wall of the box body is fixedly connected with a guide plate, and the bottom end of the guide plate is fixedly connected with the bottom end of the inner wall of the box body.

Preferably, the top right part of the feeding pipe is hinged with a baffle.

Preferably, a handle is fixedly connected to the top end of the baffle.

Preferably, the outer side wall of the handle is provided with anti-slip lines.

(III) advantageous effects

Compared with the prior art, the invention provides secondary separation and extraction equipment for antibody extraction, which has the following beneficial effects:

open first motor through the user, first motor drives the axis of rotation and rotates, the axis of rotation drives stirring vane and rotates, and then smashes the raw materials, then open the second motor through the user, the output of second motor drives the auger and rotates, the raw materials after smashing passes through the auger and gets into the connecting pipe, the extract flows in wide mouthful pipe through the apopore, open the second relief valve through the user, and then the extract flows into first case that holds, open first relief valve through the user, and then make the residue flow in the second through fixed pipe and hold the case, the residue that has reached the raw materials can separate once more with the extract, thereby can reduce the waste, the practicality is higher.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1A;

FIG. 3 is an enlarged view of a portion of FIG. 1 at B according to the present invention;

FIG. 4 is a right side view of the wide-mouth tube, the support plate and the box body of the present invention;

fig. 5 is a schematic left sectional view of the bottom plate, the male slider and the first container in the present invention.

In the figure: 1. a base plate; 2. a box body; 3. a first motor; 4. a rotating shaft; 5. a stirring blade; 6. a feed pipe; 7. a connecting pipe; 8. a second motor; 9. a packing auger; 10. a fixed tube; 11. a first discharge valve; 12. a water outlet hole; 13. a wide-mouth tube; 14. a support plate; 15. a first placing plate; 16. a convex slider; 17. a first accommodating box; 18. a second discharge valve; 19. a second placing plate; 20. a second accommodating box; 21. a reinforcing bar; 22. a baffle; 23. a baffle plate; 24. a handle.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

Referring to fig. 1-5, the invention relates to a secondary separation and extraction device for extracting antibody, comprising a bottom plate 1, a box body 2 fixedly connected to the left part of the top end of the bottom plate 1, a first motor 3 installed on the top end of the box body 2, a rotating shaft 4 fixedly connected to the output end of the first motor 3, a plurality of groups of stirring blades 5 fixedly connected to the circumferential outer wall of the rotating shaft 4, a feeding pipe 6 communicated with the left part of the top end of the box body 2, a connecting pipe 7 communicated with the lower part of the right end of the box body 2, a second motor 8 installed on the right end of the connecting pipe 7, an auger 9 fixedly connected to the output end of the second motor 8, a connecting pipe 7 communicated with the left end of the auger 9 and extending to the inside of the connecting pipe 7, a fixed pipe 10 communicated with the right part of the bottom end of the connecting pipe 7, a first discharge valve 11 installed on the middle part of the circumferential outer wall of the fixed pipe 10, a plurality of apopores 12 have been seted up to the bottom of connecting pipe 7, the wide mouthful pipe 13 of bottom fixedly connected with of connecting pipe 7, the bottom of wide mouthful pipe 13 runs through bottom plate 1 and extends to the outside, and the equal fixedly connected with backup pad 14 in bottom left part and the right part of bottom plate 1, the first board 15 of placing of the relative end fixedly connected with of each backup pad 14, the first protruding type spout of having seted up in the top right part of placing board 15, the inner wall sliding fit of protruding type spout has protruding type slider 16, the first case 17 that holds of the first top fixedly connected with of protruding type slider 16, and the second row of material valve 18 is installed to the circumference outer wall lower part of wide mouthful pipe 13, and the board 19 is placed to the right-hand member fixedly connected with second that is located the backup pad 14 of right part, the second is placed the top fixedly connected with second of board 19 and is held case 20.

Furthermore, a reinforcing rod 21 is fixedly connected to the right end of the supporting plate 14 positioned at the right part, the top end of the reinforcing rod 21 is fixedly connected with the bottom end of the second placing plate 19, the stability of the second placing plate 19 can be improved by the reinforcing rod 21, a guide plate 22 is fixedly connected to the left end of the inner wall of the box body 2, the bottom end of the guide plate 22 is fixedly connected with the bottom end of the inner wall of the box body 2, the occurrence of retention of raw materials in the box body 2 can be reduced by the guide plate 22, a baffle 23 is hinged to the right part of the top end of the feeding pipe 6, the occurrence of entering of external objects into the box body 2 can be reduced by the baffle 23, a handle 24 is fixedly connected to the top end of the baffle 23, the baffle 23 can be conveniently opened by a user through the handle 24, anti-slip patterns are arranged on the outer side wall of the handle 24, and the occurrence of falling of the handle 24 in the hand of the user can be reduced through the anti-slip patterns; open first motor 3 through the user, first motor 3 drives axis of rotation 4 and rotates, axis of rotation 4 drives stirring vane 5 and rotates, and then smashes the raw materials, then open second motor 8 through the user, second motor 8's output drives auger 9 and rotates, auger 9 gets into connecting pipe 7 through the raw materials after smashing, the extract flows into wide-mouthed pipe 13 through apopore 12, open second relief valve 18 through the user, and then the extract flows into first case 17 that holds, open first relief valve 11 through the user, and then make the residue flow into the second through fixed pipe 10 and hold case 20, the residue that has reached the raw materials can carry out the reseparation with the extract, thereby can reduce the waste, the practicality is higher.

In summary, the working principle and working process of the secondary separation and extraction equipment for antibody extraction are that when the equipment is used, firstly, a user opens the first motor 3, the first motor 3 drives the rotating shaft 4 to rotate, the rotating shaft 4 drives the stirring blade 5 to rotate, so that raw materials are crushed, then, the user opens the second motor 8, the output end of the second motor 8 drives the packing auger 9 to rotate, the crushed raw materials enter the connecting pipe 7 through the packing auger 9, the extracting solution flows into the wide-mouth pipe 13 through the water outlet hole 12, then, the user opens the second discharge valve 18, so that the extracting solution flows into the first accommodating box 17, and then, the user opens the first discharge valve 11, so that residues flow into the second accommodating box 20 through the fixing pipe 10; the first motor 3 and the second motor 8 can be products with a model number of Y60L-2, the first motor 3 and the second motor 8 are devices directly purchased from the market and known to those skilled in the art, and here we only use them, and do not improve them in structure and function, and we will not repeat here in detail, and the first motor 3 and the second motor 8 are provided with control switches matched with them, and the installation positions of the control switches are selected according to actual use requirements, so that the operation and control of an operator are facilitated.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An antibody is extracted with secondary separation extraction equipment, includes bottom plate (1), its characterized in that: the utility model discloses a bottom plate, including bottom plate (1), bottom plate, box (2), first motor (3) are installed on the top of box (2), the output end fixedly connected with axis of rotation (4) of first motor (3), the bottom of axis of rotation (4) runs through box (2) and extends to the inside of box (2), and the circumference outer wall fixedly connected with multiunit stirring vane (5) of axis of rotation (4), and the top left part intercommunication of box (2) has inlet pipe (6), and the right-hand member lower part intercommunication of box (2) has connecting pipe (7), second motor (8) are installed to the right-hand member of connecting pipe (7), the output fixedly connected with auger (9) of second motor (8), the left end of auger (9) runs through connecting pipe (7) and extends to the inside of connecting pipe (7) to the bottom right part intercommunication of connecting pipe (7) has fixed tube (10), a first material discharge valve (11) is installed in the middle of the outer circumferential wall of the fixed pipe (10), a plurality of water outlet holes (12) are formed in the bottom end of the connecting pipe (7), a wide-mouth pipe (13) is fixedly connected to the bottom end of the connecting pipe (7), the bottom end of the wide-mouth pipe (13) penetrates through the bottom plate (1) and extends to the outside, supporting plates (14) are fixedly connected to the left and right of the bottom end of the bottom plate (1), a first placing plate (15) is fixedly connected to the opposite end of each supporting plate (14), a convex sliding groove is formed in the right of the top end of the first placing plate (15), a convex sliding block (16) is matched with the inner wall of the convex sliding groove in a sliding manner, a first accommodating box (17) is fixedly connected to the top end of the convex sliding block (16), a second material discharge valve (18) is installed on the lower portion of the outer circumferential wall of the wide-mouth pipe (13), and a second placing plate (19) is fixedly connected to the right end of the supporting plate (14) positioned on the right, the top end of the second placing plate (19) is fixedly connected with a second containing box (20).

2. The apparatus for secondary separation and extraction for antibody extraction according to claim 1, wherein: and a reinforcing rod (21) is fixedly connected to the right end of the supporting plate (14) at the right part, and the top end of the reinforcing rod (21) is fixedly connected with the bottom end of the second placing plate (19).

3. The apparatus for secondary separation and extraction for antibody extraction according to claim 2, wherein: the left end of the inner wall of the box body (2) is fixedly connected with a guide plate (22), and the bottom end of the guide plate (22) is fixedly connected with the bottom end of the inner wall of the box body (2).

4. The apparatus for secondary separation and extraction for antibody extraction according to claim 3, wherein: the top right part of inlet pipe (6) articulates there is baffle (23).

5. The apparatus for secondary separation and extraction for antibody extraction according to claim 4, wherein: the top end of the baffle (23) is fixedly connected with a handle (24).

6. The apparatus for secondary separation and extraction for antibody extraction according to claim 5, wherein: the outer side wall of the handle (24) is provided with anti-skid grains.