CN114027291A - Method for treating membranous wings of insects - Google Patents
Method for treating membranous wings of insects Download PDFInfo
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- CN114027291A CN114027291A CN202111435569.7A CN202111435569A CN114027291A CN 114027291 A CN114027291 A CN 114027291A CN 202111435569 A CN202111435569 A CN 202111435569A CN 114027291 A CN114027291 A CN 114027291A
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- membranous
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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Abstract
The invention provides a method for treating insect membranous wings, which comprises the following steps: s1, separating membranous wings from the soak solution A; s2, transferring the separated membranous wings to a concave glass containing glycerol, standing to enable the membranous wings to stretch naturally, stretching the surfaces of the membranous wings by using non-sharp parts of tools, and standing for a period of time for observation; s3, pressing and fixing the spread membranous wings in glycerol; s4, cleaning the flattened membranous wings to remove glycerol; s5, placing the cleaned membranous wings in the soak solution B for observation and photographing; s6, and the membranous wings after completion of observation are stored in glycerol. Solves the problem that the membranous wing is damaged due to improper storage of a large number of specimens.
Description
Technical Field
The invention relates to the technical field of insect research, in particular to a method for treating membranous wings of insects.
Background
The wings of the insects are important characteristics for identifying species, and the structure of the wing veins is critical in the process of identifying families, genera and species. Typically, specimens collected in the field are soaked in ethanol and brought back to the laboratory for further study. The samples collected at one time are various, and a large number of samples are mutually extruded in the process of being stored in the ethanol bottle, so that the fragile and easily folded membranous wings are easy to bend and deform. When the wing vein is examined under a microscope, the observation of the wing vein can be influenced, and the later photographing of the wing vein is not facilitated.
The existing processing method cannot solve the problem that the membranous wing is damaged due to improper storage of a large number of specimens, so that the specimens with folded wings cannot be well identified and photographed at a later stage. A complete picture of the vein can embody the vein characteristics, which has important classification significance in insect groups. Therefore, there is a need to provide a method for processing hymenoptera of diptera and hymenoptera insects, which can solve the problem that the hymenoptera of diptera and hymenoptera insects cannot be identified and photographed at a later stage well under the condition of extrusion, and the identification of local characteristics of insects and photographing are greatly disturbed.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a method for treating the membranous wing of an insect, which solves the problem that the membranous wing is damaged due to improper storage of a large number of specimens.
In order to achieve the above object, the technical solution of the present invention is as follows.
A method for treating insect membranous wings comprises the following steps:
s1, separating membranous wings from the soak solution A;
s2, transferring the separated membranous wings to a concave glass containing glycerol, standing to enable the membranous wings to stretch naturally, stretching the surfaces of the membranous wings by using non-sharp parts of tools, and standing for a period of time for observation;
s3, pressing and fixing the spread membranous wings in glycerol;
s4, cleaning the flattened membranous wings to remove glycerol;
s5, placing the cleaned membranous wings in the soak solution B for observation and photographing;
s6, and the membranous wings after completion of observation are stored in glycerol.
Further, in step S1, the soak solution a is a 75% ethanol solution.
Further, in step S2, the time for the natural unfolding is 20 to 50 seconds by standing.
Further, in step S2, the standing is performed for 1-2 min.
Further, step S2 includes repeating step S2 to further adjust the posture.
Further, in step S3, the pressing is performed by pressing the membranous wing with a non-sharp portion of the tool.
Further, in step S3, the pressing time is 2-15 min.
Further, in step S4, the cleaning is performed by placing the flattened membranous wing in a cleaning solution, wherein the cleaning solution is ethanol.
Further, in step S5, the soak solution B is 75% ethanol or glycerol.
The invention has the beneficial effects that:
1. the method treats the insect membranous wings through the steps of pre-shearing, spreading, posture rectification, fixing, cleaning, observing and storing, and the treated insect membranous wings can be preserved for a long time and can be used for observing and photographing specimens.
2. The hymenoptera of the insect treated by the method can obtain clear veins which are used as the most important evidence for identifying diptera, hymenoptera and other insects, can provide a long evidence basis for long-term storage, can also be used as a model specimen for storage, provides an important morphological basis for later research, provides evidence for identifying species and similar species, and plays an important role in scientific research and rapid identification.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention, the adopted materials and equipment can be purchased in the market if no special description is provided.
Example 1
S1, pre-cutting
Cutting off the basal part of the membranous wing with ophthalmic scissors in 75% ethanol solution to obtain separated membranous wing; or when the membranous wing base is too fine, using a sharp-pointed forceps to clamp the membranous wing base, and slightly pulling down the membranous wing base to obtain a separated membranous wing;
the membrane separation method is characterized in that the membrane separation is carried out in a 75% ethanol solution, so that the membrane separation is carried out in order to achieve the effects of cleaning and sterilization, avoid the influence of over-high concentration ethanol (100% ethanol) on color degradation, and facilitate better separation due to better light transmittance of the 75% ethanol.
S2, spreading and adjusting posture
Transferring the separated membranous wing to a concave glass containing glycerol, and standing for about 30s to enable the membranous wing to naturally spread; then, stretching the surface of the membranous wing by using a non-sharp part of the elbow tweezers or a circular ring part of the inoculating loop so as to achieve an ideal stretching state, such as flatness and no wrinkles, standing for 1min, and observing;
wherein, the glycerol mainly plays a role in fixing.
S3, fixing posture
Pressing and fixing the spread membranous wing in glycerol by using insects aiming at the round head part or similar non-sharp objects, and pressing the membranous wing for about 3min to obtain the manufactured flat membranous wing;
s4, cleaning
Taking out the prepared flat membranous wing by using a common forceps, and placing the membranous wing in 100% ethanol for cleaning to remove glycerol; wherein the glycerol is neutralized with 100% ethanol to prepare for the next photographing in ethanol. The purpose of washing off glycerol with ethanol is mainly due to the poor light transmission of glycerol, which is not conducive to subsequent observation and shooting.
S5, observation
Storing the cleaned membranous wings in a medium of 75% ethanol for observation and photographing;
among them, 75% ethanol can reduce damage to the membranous wings caused by ethanol with too high concentration compared with 100% ethanol, mainly because the color degradation is affected by ethanol with too high concentration, thereby causing color damage to the membranous wings. While the light transmittance of ethanol itself is relatively good compared to that of glycerol.
S6, storage
The observed membranous wing can be placed in a micro centrifugal tube or a common centrifugal tube filled with glycerol for long-term preservation. The specific size of the centrifuge tube can be determined according to the size of the membranous wing.
The purpose of using glycerin is to enable the soft membranous wing to achieve the effects of dehydration, corrosion prevention and fixation. The glycerol is beneficial to the preservation of small and easily damaged organs, and the ethanol or formaldehyde adopted in the prior method is easy to fade along with the prolonging of the preservation time, while the glycerol is not easy to fade.
Example 2
S1, pre-cutting
Cutting off the basal part of the membranous wing with ophthalmic scissors in 75% ethanol solution to obtain separated membranous wing; or when the membranous wing base is too fine, using a sharp-pointed forceps to clamp the membranous wing base, and slightly pulling down the membranous wing base to obtain a separated membranous wing;
s2, spreading and adjusting posture
Transferring the separated membranous wing to a concave glass containing glycerol, and standing for about 20s to enable the membranous wing to naturally spread; then, stretching the surface of the membranous wing by using a non-sharp part of the elbow tweezers or a circular ring part of the inoculating loop so as to achieve an ideal stretching state, such as flatness and no wrinkles, standing for 1min, and observing;
and (5) repeating the step S2 to further adjust the posture, wherein the specific operation is as follows:
standing for about 20s to allow the fabric to naturally unfold; then, stretching the surface of the membranous wing by using a non-sharp part of the elbow tweezers or a circular ring part of the inoculating loop so as to achieve an ideal stretching state, such as flatness and no wrinkles, standing for 1min, and observing;
s3, fixing posture
Pressing and fixing the spread membranous wing in glycerol by using insects aiming at the round head part or similar non-sharp objects, and pressing the membranous wing for about 3min to obtain the manufactured flat membranous wing;
s4, cleaning
Taking out the prepared flat membranous wing by using a common forceps, and placing the membranous wing in 100% ethanol for cleaning to remove glycerol; wherein, the 100% ethanol can neutralize the glycerol and prepare for the next photographing.
S5, observation
Storing the cleaned membranous wings in glycerol for observation and photographing;
because the glycerol medium is viscous, careful handling is required to avoid damage to the membranous wings. The glycerol medium adopted in the embodiment has the advantages of being convenient for fixation during photographing, but the transparency is lower than that of an ethanol solution. Ethanol itself is much more translucent than glycerol, and therefore, photographs were taken in ethanol in most cases. Since glycerol has a better fixing effect than ethanol but a lower light transmittance, 75% ethanol can be considered for a heavy color of membranous wings, and a glycerol medium can be considered for soft membranous wings.
S6, storage
The observed membranous wing can be placed in a micro centrifugal tube or a common centrifugal tube filled with glycerol for long-term preservation. The specific size of the centrifuge tube can be determined according to the size of the membranous wing.
Example 3
S1, pre-cutting
Cutting off the basal part of the membranous wing with ophthalmic scissors in 75% ethanol solution to obtain separated membranous wing; or when the membranous wing base is too fine, using a sharp-pointed forceps to clamp the membranous wing base, and slightly pulling down the membranous wing base to obtain a separated membranous wing;
s2, spreading and adjusting posture
Transferring the separated membranous wing to a concave glass containing glycerol, and standing for about 50s to enable the membranous wing to naturally spread; then, stretching the surface of the membranous wing by using a non-sharp part of the elbow tweezers or a circular ring part of the inoculating loop so as to achieve an ideal stretching state, such as flatness and no wrinkles, standing for 2min, and observing;
s3, fixing posture
Pressing and fixing the spread membranous wing in glycerol by using insects aiming at the round head part or similar non-sharp objects, and pressing the membranous wing for about 2min to obtain the manufactured flat membranous wing;
s4, cleaning
Taking out the prepared flat membranous wing by using a common forceps, and placing the membranous wing in 100% ethanol for cleaning to remove glycerol; wherein, the 100% ethanol can neutralize the glycerol and prepare for the next photographing.
S5, observation
Storing the cleaned membranous wings in 75% ethanol for observation and photographing;
s6, storage
The observed membranous wing can be placed in a micro centrifugal tube or a common centrifugal tube filled with glycerol for long-term preservation. The specific size of the centrifuge tube can be determined according to the size of the membranous wing.
The methods of embodiments 1 to 3 of the present invention are suitable for the production of a majority of damaged membranous wings of the order diptera and hymenoptera (usually 1 to 4mm), and the methods of embodiments 1 to 3 have been verified and have achieved good results on hermetia illucens, mythidae and other groups.
In the methods of examples 1 to 2, the membranous wings were observed in different media, as shown in table 1.
TABLE 1 media used for the observations of examples 1-3
Note: 1-2 in the light transmission sequence respectively represent the sequence from high light transmission to low light transmission; and 1-2 in the viscosity sequence respectively represent the sequence from high to low in viscosity.
It is clear from the observation of the membranous wings of examples 1-2 that the use of 75% ethanol in example 1 is more translucent than the glycerol medium of example 2, and that damage to the membranous wing color by high concentration ethanol (100% ethanol) can be avoided. The glycerol medium adopted in the embodiment 2 is beneficial to fixing the membranous wing during photographing due to the self-viscosity property. Therefore, the corresponding medium can be selected according to the soft characteristic and the color characteristic of the membranous wing for observation and photographing.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A method for treating an insect membranous wing is characterized by comprising the following steps:
s1, separating membranous wings from the soak solution A;
s2, transferring the separated membranous wings to a concave glass containing glycerol, standing to enable the membranous wings to stretch naturally, stretching the surfaces of the membranous wings by using non-sharp parts of tools, and standing for a period of time for observation;
s3, pressing and fixing the spread membranous wings in glycerol;
s4, cleaning the flattened membranous wings to remove glycerol;
s5, placing the cleaned membranous wings in the soak solution B for observation and photographing;
s6, and the membranous wings after completion of observation are stored in glycerol.
2. The method for treating insect hymenoptera according to claim 1, wherein the soaking solution A is a 75% ethanol solution in step S1.
3. The method for treating membranous wings of insects as claimed in claim 1, wherein the time taken for the membranous wings to naturally spread by standing in step S2 is 20-50S.
4. The method for treating membranous wings of insects as claimed in claim 1, wherein the standing time in step S2 is 1-2 min.
5. The method of claim 1, further comprising repeating step S2 for further pose adjustment in step S2.
6. The method for treating insect hymenopterans according to claim 1, wherein the pressing in step S3 is performed by pressing the hymenopterans with a non-sharp portion of a tool.
7. The method for treating membranous wings of insects as claimed in claim 1, wherein the pressing time in step S3 is 2-15 min.
8. The method for treating insect hymenopterans according to claim 1, wherein the washing in step S4 is carried out by placing the flattened hymenopterans in a washing liquid, said washing liquid being ethanol.
9. The method for treating insect hymenoptera according to claim 1, wherein the soaking solution B is 75% ethanol or glycerin in step S5.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111435569.7A CN114027291B (en) | 2021-11-29 | 2021-11-29 | Method for treating membranous wings of insects |
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| CN202111435569.7A CN114027291B (en) | 2021-11-29 | 2021-11-29 | Method for treating membranous wings of insects |
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| CN114027291A true CN114027291A (en) | 2022-02-11 |
| CN114027291B CN114027291B (en) | 2022-11-01 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105427728A (en) * | 2016-01-19 | 2016-03-23 | 金陵科技学院 | Method for removing wing surface scales of lepidopterous insects |
| US20210345602A1 (en) * | 2018-09-28 | 2021-11-11 | Otsuka Pharmaceutical Factory, Inc | Mammal cell preserving solution containing acarbose or stachyose |
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2021
- 2021-11-29 CN CN202111435569.7A patent/CN114027291B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105427728A (en) * | 2016-01-19 | 2016-03-23 | 金陵科技学院 | Method for removing wing surface scales of lepidopterous insects |
| US20210345602A1 (en) * | 2018-09-28 | 2021-11-11 | Otsuka Pharmaceutical Factory, Inc | Mammal cell preserving solution containing acarbose or stachyose |
Non-Patent Citations (2)
| Title |
|---|
| 任淑仙等: "《动物的类群》", 31 December 1983, 人民教育出版社 * |
| 程宝绰等: "《昆虫博士》", 31 December 1991, 知识出版社 * |
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