CN114018677A - Method for extracting potassium ions in liquid flow of woody part - Google Patents
Method for extracting potassium ions in liquid flow of woody part Download PDFInfo
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- CN114018677A CN114018677A CN202111291430.XA CN202111291430A CN114018677A CN 114018677 A CN114018677 A CN 114018677A CN 202111291430 A CN202111291430 A CN 202111291430A CN 114018677 A CN114018677 A CN 114018677A
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- 239000007788 liquid Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 20
- 229910001414 potassium ion Inorganic materials 0.000 title claims abstract description 20
- 210000000020 growth cone Anatomy 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000010257 thawing Methods 0.000 claims abstract description 6
- 230000008014 freezing Effects 0.000 claims abstract description 5
- 238000007710 freezing Methods 0.000 claims abstract description 5
- 238000003860 storage Methods 0.000 claims abstract description 5
- 235000009161 Espostoa lanata Nutrition 0.000 claims abstract description 4
- 240000001624 Espostoa lanata Species 0.000 claims abstract description 4
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 150000002500 ions Chemical class 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000009413 insulation Methods 0.000 claims abstract description 3
- 239000003344 environmental pollutant Substances 0.000 claims abstract 2
- 231100000719 pollutant Toxicity 0.000 claims abstract 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 8
- 239000000356 contaminant Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 240000000528 Ricinus communis Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting potassium ions from a woody part liquid flow, which comprises the steps of chiseling barks at four azimuth angles of a trunk by using a chisel, spraying deionized water on exposed xylem, and sucking the exposed xylem by using a sterile cotton ball to remove pollutants in the phloem liquid flow; then collecting xylem samples by using a growth cone, sealing the xylem samples in a sterile self-sealing bag, and storing all the samples in an insulation can until the samples are treated in a laboratory; thawing xylem sample stored in a laboratory refrigerator, cutting into small blocks by using a sterile blade, vertically placing the sample in a centrifuge tube and centrifuging, extracting xylem liquid from the sample, sucking out the xylem liquid by using a disposable needle tube, filtering, filling the xylem liquid into a freezing tube, marking, and quickly transferring the xylem liquid into the refrigerator for storage until the potassium ion concentration of the xylem liquid is chemically analyzed by using an ion chromatograph. The extraction method only needs to collect a small amount of xylem samples when collecting the xylem samples, is simple and efficient, can finish the collection work by one person by means of the growth cone, and has little damage to trees.
Description
Technical Field
The invention relates to a method for extracting potassium ions from a woody plant woody part liquid flow.
Background
Potassium ions are involved in the activation of more than 60 enzyme systems in the growth and development process of plants, and are closely related to various metabolic processes such as photosynthesis, respiration, carbohydrate, fat and protein synthesis and the like. The xylem of the trunk is a main channel for potassium ions absorbed by the root of the tree to be transported to the leaves, and the change of the concentration of the potassium ions contains very important forest plant physiological and ecological information. At present, the research on the chemical components of the sap of the trunk and the sap is mainly based on indoor culture experiments of model plants (such as castor-oil plants) or tree seedlings, and the sap flow of the trunk and the sap of the plants is extracted by adopting a vacuum extraction method, a root pressure method and a pressure chamber method, but the methods have the defects of small acquisition amount, large destructiveness, difficult operability and the like, are not suitable for acquiring the sap flow of the trunk and the sap of forest trees (particularly adult trees) under natural in-situ conditions, and seriously restrict the application of the variation characteristics of the concentration of potassium ions in the sap flow of the trunk and the sap flow in the diagnosis and evaluation of the physiological processes and the growth conditions of the forest plants.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for extracting potassium ions from the liquid flow of the woody plant woody part, which solves the problems of small acquisition amount, large destructiveness, difficult operability and the like of the method for extracting the liquid flow of the woody plant trunk woody part in the prior art.
The technical scheme of the invention is as follows:
a method for extracting potassium ion from liquid of xylem comprises chiseling bark with chisel at four azimuth angles of trunk, spraying deionized water on exposed xylem, and sucking with sterile cotton ball to remove contaminants in the liquid of phloem; then collecting xylem samples by using a growth cone, sealing the xylem samples in a sterile self-sealing bag, and storing all the samples in an insulation can until the samples are treated in a laboratory; thawing xylem sample stored in a laboratory refrigerator, cutting into small blocks by using a sterile blade, vertically placing the sample in a centrifuge tube and centrifuging, extracting xylem liquid from the sample, sucking out the xylem liquid by using a disposable needle tube, filtering, filling the xylem liquid into a freezing tube, marking, and quickly transferring the xylem liquid into the refrigerator for storage until the potassium ion concentration of the xylem liquid is chemically analyzed by using an ion chromatograph.
Further, the chisel has a bark removal height of 1.3m and a bark size of 1.5cm × 1.5cm per azimuth;
further, the length of the xylem sample collected by the growth cone is 4-6cm, the number of the xylem samples in each azimuth angle is 2-3, and the total number is 8-12;
further, the sample is stored in an incubator at 4 ℃, laboratory at-40 ℃ and thawing at 4 ℃;
furthermore, the size of the centrifugal tube is 50ml, the rotating speed is 11000rpm, the time is set to be 20min, and the aperture of the filter membrane is 0.45 mu m.
Compared with the prior art, the method has the following beneficial effects:
the extraction method only needs to collect a small amount of xylem samples when collecting the xylem samples, is simple and efficient, can finish the collection work by one person by means of the growth cone, and has little damage to trees. The collected xylem sample is subjected to freezing, thawing, dicing, centrifuging, filtering and other steps to extract the xylem liquid flow potassium-containing solution, and compared with other methods, the method has the advantages of accurate potassium ion concentration value, simple operation, high efficiency, science and contribution to popularization and utilization.
Drawings
FIG. 1 is a flow chart of the method of the present invention.
Detailed Description
The present invention is further illustrated by the following specific examples.
The test methods used in the following examples are as follows:
removing 1.5cm x 1.5cm bark with a chisel at four azimuths of about 1.3 meters of the trunk, spraying deionized water on the exposed xylem and blotting with a sterile cotton ball to remove contaminants from the phloem liquor;
drilling xylem samples with the length of about 4-6cm by using a growth cone, wherein the number of the samples at each azimuth angle is 2-3, the total number is 8-12, the obtained xylem samples are placed into a sterile self-sealing bag to be sealed, and all the samples are stored in an incubator at 4 ℃ until the samples are treated in a laboratory;
the temperature of the sample in a refrigerator of a laboratory is kept below-40 ℃, the sample is unfrozen in the refrigerator at 4 ℃ before being treated, the unfrozen xylem sample is cut into small blocks by a sterile blade, then the small blocks are placed in a 50ml centrifuge and centrifuged for 20min at 11000rpm, the xylem liquid flow extracted from the sample is sucked out by a disposable needle tube and filtered by a filter head with the diameter of 0.45 mu m, then the sample is put into a freezing tube with the volume of 5ml and marked, and the sample is rapidly transferred to the refrigerator at-40 ℃ for storage;
the treated liquid flow sample was thawed in a refrigerator at 4 ℃ and analyzed for potassium ion concentration using an ion chromatograph.
Claims (5)
1. A method for extracting potassium ions from a woody part liquid flow is characterized in that barks are chiseled at four azimuth angles of a trunk by a chisel, deionized water is sprayed on exposed xylem, and the exposed xylem is sucked dry by a sterile cotton ball to remove pollutants in the phloem liquid flow; then collecting xylem samples by using a growth cone, sealing the xylem samples in a sterile self-sealing bag, and storing all the samples in an insulation can until the samples are treated in a laboratory; thawing xylem sample stored in a laboratory refrigerator, cutting into small blocks by using a sterile blade, vertically placing the sample in a centrifuge tube and centrifuging, extracting xylem liquid from the sample, sucking out the xylem liquid by using a disposable needle tube, filtering, filling the xylem liquid into a freezing tube, marking, and quickly transferring the xylem liquid into the refrigerator for storage until the potassium ion concentration of the xylem liquid is chemically analyzed by using an ion chromatograph.
2. The method of extracting potassium ions from a woody portion stream according to claim 1, wherein the chisel removes bark having a height of 1.3m and a bark size of 1.5cm x 1.5cm per azimuth.
3. The method for extracting potassium ions from a xylem fluid according to claim 1, wherein the length of the xylem sample collected by the growth cone is 4-6cm, and the number of xylem samples per azimuth angle is 2-3, and the total number is 8-12.
4. The method for extracting potassium ions from a woody portion stream according to claim 1, wherein the sample is stored in an incubator at a temperature of 4 ℃, a laboratory storage temperature of-40 ℃ and a thawing temperature of 4 ℃.
5. The method for extracting the potassium ions in the liquid flow of the xylem is characterized in that the size of the centrifugal tube is 50ml, the rotating speed is 11000rpm, the time is set to be 20min, and the aperture of the filter membrane is 0.45 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111291430.XA CN114018677A (en) | 2021-11-01 | 2021-11-01 | Method for extracting potassium ions in liquid flow of woody part |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111291430.XA CN114018677A (en) | 2021-11-01 | 2021-11-01 | Method for extracting potassium ions in liquid flow of woody part |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114018677A true CN114018677A (en) | 2022-02-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111291430.XA Pending CN114018677A (en) | 2021-11-01 | 2021-11-01 | Method for extracting potassium ions in liquid flow of woody part |
Country Status (1)
| Country | Link |
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| CN (1) | CN114018677A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063655A (en) * | 2012-12-28 | 2013-04-24 | 中国科学院新疆生态与地理研究所 | Method for detecting mineral nutrient cycling from cotton seedling stage to cotton flowering and boll-forming stage by applying pressure |
| CN106546461A (en) * | 2016-11-07 | 2017-03-29 | 赣南师范大学 | A kind of Citrus phloem sap preparation method |
-
2021
- 2021-11-01 CN CN202111291430.XA patent/CN114018677A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103063655A (en) * | 2012-12-28 | 2013-04-24 | 中国科学院新疆生态与地理研究所 | Method for detecting mineral nutrient cycling from cotton seedling stage to cotton flowering and boll-forming stage by applying pressure |
| CN106546461A (en) * | 2016-11-07 | 2017-03-29 | 赣南师范大学 | A kind of Citrus phloem sap preparation method |
Non-Patent Citations (1)
| Title |
|---|
| LINGLING CHEN.ET AL: ""Characteristics of chemical components in the trunk xylem sap of pine trees by means of a centrifugation collection method"", PLANT PHYSIOLOGY AND BIOCHEMISTRY, pages 482 - 489 * |
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