CN113999330A - Low-salt-concentration heparin sodium and active intestinal protein peptide separation and co-production process - Google Patents
Low-salt-concentration heparin sodium and active intestinal protein peptide separation and co-production process Download PDFInfo
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 91
- 229920000669 heparin Polymers 0.000 title claims abstract description 88
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 86
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 title claims abstract description 68
- 229960001008 heparin sodium Drugs 0.000 title claims abstract description 65
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 57
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 35
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 238000000926 separation method Methods 0.000 title claims abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 58
- 229920005989 resin Polymers 0.000 claims abstract description 58
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 210000004347 intestinal mucosa Anatomy 0.000 claims abstract description 47
- 238000001179 sorption measurement Methods 0.000 claims abstract description 40
- 238000001035 drying Methods 0.000 claims abstract description 30
- 210000000936 intestine Anatomy 0.000 claims abstract description 24
- 150000003839 salts Chemical class 0.000 claims abstract description 21
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 20
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 20
- 238000010828 elution Methods 0.000 claims abstract description 16
- 230000007935 neutral effect Effects 0.000 claims abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 10
- 238000001556 precipitation Methods 0.000 claims abstract description 10
- 102000035195 Peptidases Human genes 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 210000000813 small intestine Anatomy 0.000 claims abstract description 7
- 239000003480 eluent Substances 0.000 claims description 99
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 70
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 51
- 238000000034 method Methods 0.000 claims description 44
- 238000003756 stirring Methods 0.000 claims description 44
- 238000010438 heat treatment Methods 0.000 claims description 37
- 239000011780 sodium chloride Substances 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 31
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 20
- 229960002897 heparin Drugs 0.000 claims description 20
- 239000011259 mixed solution Substances 0.000 claims description 20
- 230000002255 enzymatic effect Effects 0.000 claims description 17
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 238000005406 washing Methods 0.000 claims description 16
- 239000000413 hydrolysate Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000008213 purified water Substances 0.000 claims description 13
- 239000000047 product Substances 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000001291 vacuum drying Methods 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 239000003957 anion exchange resin Substances 0.000 claims description 6
- PGWTYMLATMNCCZ-UHFFFAOYSA-M azure A Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 PGWTYMLATMNCCZ-UHFFFAOYSA-M 0.000 claims description 5
- 239000012043 crude product Substances 0.000 claims description 5
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 claims description 4
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 claims description 4
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 2
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 claims description 2
- 229940001584 sodium metabisulfite Drugs 0.000 claims description 2
- 235000010262 sodium metabisulphite Nutrition 0.000 claims description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims 2
- 235000010265 sodium sulphite Nutrition 0.000 claims 1
- 239000002351 wastewater Substances 0.000 abstract description 12
- 229920002683 Glycosaminoglycan Polymers 0.000 abstract description 8
- 230000007062 hydrolysis Effects 0.000 abstract description 7
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 7
- 238000005904 alkaline hydrolysis reaction Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
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- 210000004877 mucosa Anatomy 0.000 abstract 1
- 230000033228 biological regulation Effects 0.000 description 16
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- 238000007689 inspection Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
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- 230000022676 rumination Effects 0.000 description 5
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- 241000282887 Suidae Species 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 229940127215 low-molecular weight heparin Drugs 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000002785 anti-thrombosis Effects 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
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- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical class FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
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- 206010062713 Haemorrhagic diathesis Diseases 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
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- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
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- 210000004400 mucous membrane Anatomy 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
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- 239000012588 trypsin Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention relates to the field of biological pharmacy, in particular to a separation and co-production process of low-salt-concentration heparin sodium and active intestinal protein peptide. Fresh pig small intestine mucosa is used as an initial raw material, a sausage casing is peeled by a intestine scraper to obtain the intestinal mucosa, and an antioxidant protective agent is added to protect mucosal protein so as to improve the additional value of the intestinal mucosal protein peptide. Under the condition of low salt concentration, the protein and the glycosaminoglycan are efficiently dissociated by combining neutral compound proteolytic enzyme hydrolysis and high-temperature alkaline hydrolysis, and then physical operations such as resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like are adopted, so that the co-production of crude heparin sodium and active intestinal protein is finally realized. The co-production process is beneficial to less discharge of waste water containing salt and the like, improves the added value of products and has important economic value.
Description
Technical Field
The invention relates to the field of biological pharmacy, in particular to a separation and co-production process of low-salt-concentration heparin sodium and active intestinal protein peptide.
Background
Heparin sodium belongs to glycosaminoglycan molecules with complex structures and is the most effective anticoagulant and antithrombotic medicament clinically. The Chinese pharmacopoeia 2020 edition stipulates that raw materials for producing the heparin sodium are from healthy porcine small intestinal mucosa. China is a pig slaughtering country and controls about 70% of the upstream industrial chain of heparin raw materials worldwide. The mucous membrane of the small intestine of the pig mainly contains protein, glycosaminoglycan, nucleic acid, lipid substances and the like, wherein glycosaminoglycan and protein are combined by covalent bonds and are also called proteoglycan. The production process of the crude heparin sodium requires that protein and glycosaminoglycan are dissociated firstly, and then the crude heparin sodium is prepared through the steps of resin adsorption elution, alcohol precipitation, drying and the like. There are two common methods for extracting and dissociating crude heparin sodium: enzymolysis and salting out.
The technical route of the enzymolysis method is as follows: (1) scraping intestines and extracting intestinal mucosa (2) for enzymolysis: adding 2 degree saline into the mixed solution of intestinal mucosa, adjusting pH to 8.0-9.0 with sodium hydroxide solution, controlling the temperature of the mixed solution at 35-40 deg.C, adding trypsin, keeping the temperature for 4-6h, and heating for inactivating. (3) Separation: separating denatured protein, oil and fat from the enzymatic hydrolysate. (4) Cooling to about 50 ℃. (5) Adsorption: adjusting the pH value of the enzymolysis liquid to be more than 8.0, and the salinity is lower than 2.7 percent. Resin stirring adsorption is carried out at 50 ℃. (6) Collecting resin: collecting the adsorbed resin with a resin collecting pipe, and washing with purified water to remove impurities such as protein and oil. (7) And (3) elution: washed with 15% saline and the eluate was collected. (8) And (5) carrying out alcohol precipitation. (9) Drying and crushing.
The technical route of the salt decomposition method comprises the following steps: (1) intestinal mucosa extraction by scraping intestine (2) salt lysis: adding 4 degree saline into the mixed solution of intestinal mucosa, adjusting pH to 8.0-9.0 with sodium hydroxide solution, controlling the temperature of the mixed solution at 58 deg.C, and maintaining the temperature for 4-6 h. (3) Adsorption: adjusting the pH value of the enzymolysis liquid to be more than 8.0, and the salinity is lower than 2.7 percent. Resin stirring adsorption is carried out at 50 ℃. (4) Collecting resin: collecting the adsorbed resin with a resin collecting pipe, and washing with purified water to remove impurities such as protein and oil. (5) And (3) elution: washed with 15% saline and the eluate was collected. (6) And (5) carrying out alcohol precipitation. (7) Drying and crushing.
The production processes of the enzymolysis method and the salt hydrolysis method are two production processes commonly used in the industry at present, and because the technological process adopts higher salinity and temperature, the denaturation damage is caused to the intestinal mucosa protein, and the denatured intestinal protein is mainly used as the feed for animal husbandry. The molecular weight of the crude heparin sodium produced by the salt decomposition method is high, and the subsequent production of the heparin sodium bulk drug and the low-molecular heparin bulk drug is easily influenced by partial batches. The yield of the heparin sodium produced by the enzymolysis method is low, so that the production cost is high. The salinity of the production wastewater generated by the salt decomposition method and the enzymolysis method is higher (higher than 10000ppm) and can reach more than 5 times of that of the domestic sewage, thus causing larger environmental protection pressure.
CN103724456 discloses a normal temperature salt-free extraction process of heparin sodium, however, the process has the following disadvantages: the components such as nuclease, lipase and the like in the complex enzyme have overhigh cost, and the proteoglycan is slowly dissociated under the normal temperature condition and the reaction is not complete.
Disclosure of Invention
In order to solve the technical problems, the invention provides a separation and coproduction process of low-salt concentration heparin sodium and active intestinal protein peptide, which takes fresh pig small intestinal mucosa as a starting material, strips a sausage casing by a intestine scraper to obtain intestinal mucosa, and adds an antioxidant protective agent to protect mucosal protein so as to improve the additional value of the intestinal mucosal protein peptide. Under the condition of low salt concentration, the protein and the glycosaminoglycan are efficiently dissociated by combining neutral compound proteolytic enzyme hydrolysis and high-temperature alkaline hydrolysis, and then physical operations such as resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like are adopted, so that the co-production of crude heparin sodium and active intestinal protein is finally realized.
The technical scheme for solving the problems is as follows:
a process for separating and coproducing low-salt-concentration heparin sodium and active intestinal protein peptide comprises the following process steps: fresh small pig intestines are taken as raw materials, intestine casings are peeled off through an intestine scraper to obtain intestinal mucosa, antioxidant is added to protect mucosal protein, and the joint production of crude heparin sodium and active intestinal protein is realized through operations of low salinity enzymolysis, resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like.
The invention has the following beneficial effects:
(1) the invention combines the neutral compound protease hydrolysis and alkaline condition hydrolysis to effectively dissociate glycosaminoglycan and protein, thereby avoiding the generation of high salinity wastewater and ensuring that the salinity of the wastewater is lower than 2000 ppm.
(2) According to the invention, the antioxidant is added into the intestinal mucosa breaking liquid to perform antioxidant protection on the intestinal protein, so that the intestinal mucosa protein is effectively protected, and the commercial value of the byproduct intestinal mucosa protein peptide is improved.
(3) The separation step of the invention adopts resin adsorption and gradient salinity elution processes, thus realizing the co-production of the crude heparin and the intestinal mucosa protein peptide, reducing the production cost and improving the product value of the whole upstream industrial chain of the crude heparin.
Drawings
FIG. 1 shows a process for separating and co-producing crude heparin sodium and active intestinal protein under the condition of low salt concentration.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
A process for separating and coproducing crude heparin sodium and active intestinal protein under the condition of low salt concentration comprises the following process steps: fresh small pig intestines are taken as raw materials, intestine casings are peeled off through an intestine scraper to obtain intestinal mucosa, antioxidant is added to protect mucosal protein, and the joint production of crude heparin sodium and active intestinal protein is realized through operations of low salinity enzymolysis, resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like.
The specific process steps are as follows:
s1, intestinal mucosa extraction: taking 100 fresh small intestines of pigs, peeling off casings by using a intestine scraper to obtain intestinal mucosa, simultaneously washing with purified water, collecting intestinal mucosa mixed liquor 224L, and adding 224g of sodium metabisulfite into the intestinal mucosa mixed liquor to perform antioxidation protection on intestinal proteins.
S2, low-salinity enzymolysis: pumping the intestinal mucosa mixed solution obtained in the step S1 into an enzymolysis tank by a pump, adding 6mol/L sodium hydroxide solution to adjust the pH value range of the mixed solution to 6.0, heating and stirring, controlling the temperature to 45 ℃, adding 112g of Novoxil neutral compound proteolytic enzyme, stirring and carrying out enzymolysis for 4 hours. Adding sodium hydroxide solution to adjust the pH value to 9.0, raising the temperature of the feed liquid to 85 ℃, and continuing stirring for 1 h. Adding concentrated hydrochloric acid into the feed liquid to adjust the pH value to 7.5, and cooling to 50 ℃.
S3, resin adsorption: pumping the enzymatic hydrolysate obtained in the step S2 into an adsorption tank by a pump, adding treated A98 type anion exchange resin, heating to maintain the temperature of the feed liquid at 50 ℃, stirring for 1h until azure A test paper detects that the enzymatic hydrolysate does not show red, opening a filter valve below an adsorption tube for filtration, collecting the enzymatic hydrolysate, further washing the resin by purified water with 3 times of the volume of the resin, collecting washing liquid, and combining the washing liquid with the enzymatic hydrolysate.
S4, gradient salinity elution: firstly, preparing 2.0% sodium chloride eluent with 3 times of resin volume and 15% sodium chloride eluent with 3 times of resin volume, heating to 50 ℃, firstly adding 2.0% sodium chloride eluent with 1.5 times of resin volume into an adsorption tank, heating to 55 ℃, stirring for 1h, collecting eluent 1, adding 2.0% sodium chloride eluent with 1.5 times of resin volume into the adsorption tank again, heating to 55 ℃, stirring for 1h, collecting eluent 2, merging eluent 1 and eluent 2, and merging eluent 1, eluent 2 and the enzymolysis liquid obtained in the step S3 to obtain total protein eluent; then adding 15% sodium chloride eluent with the volume 1.5 times of that of the resin into the adsorption tank, heating to 55 ℃, stirring for 1h, and collecting eluent 3. Then adding 15% sodium chloride eluent with the volume 1.5 times of the resin volume again, heating to 55 ℃, stirring for 1h, and collecting eluent 4. And combining the eluent 3 and the eluent 4 to obtain the crude heparin sodium eluent.
S5, preparation of active intestinal protein peptide: and (4) adding ethanol with the volume of 1.5 times into the total protein eluent obtained in the step (S4), centrifugally separating by a continuous flow centrifuge to obtain an active intestinal protein wet product, then transferring the active intestinal protein wet product into a reduced pressure vacuum drying oven for drying for 3 hours, and crushing to obtain 1210g of active intestinal protein peptide powder.
S6, preparation of crude heparin sodium: and (4) adding 1.5 times of ethanol by volume into the crude heparin eluent obtained in the step (S4), standing for 4 hours, and removing the supernatant. And (3) adding ethanol into the thick heparin crude product at the bottom for dehydration, collecting powdery crude heparin sodium, transferring the powdery crude heparin sodium into a reduced-pressure vacuum drying oven for drying for 3 hours, and crushing to obtain 98g of crude heparin sodium.
The process flow diagram of this example is shown in figure 1.
The crude heparin, intestinal mucosa protein peptide and production wastewater prepared in this example were tested for salt content, and the test results are shown in tables 1-3:
TABLE 1 crude heparin sodium assay results
TABLE 2 intestinal mucin peptide assay results
Inspection item | Internal control standard | The result of the detection |
Nitrogen content | 15-20% | 16% |
Loss on drying | ≤10% | 2.4% |
Chloride compound | ≤50ppm | Compliance with regulations |
Heavy metals | ≤30ppm | Compliance with regulations |
pH value | 5.0-7.0 | 6.5 |
Rumination gene | Negative of | Compliance with regulations |
Oversulfated chondroitin sulfate | Is lower than the detection limit | Compliance with regulations |
Table 3 test results of production wastewater
Inspection item | Internal control standard | The result of the detection |
Sodium chloride content | ≤2000ppm | 1820ppm |
Ammonia nitrogen | ≤8 | 6.2 |
COD | ≤60 | 52.3 |
Example 2
A process for separating and coproducing crude heparin sodium and active intestinal protein under the condition of low salt concentration comprises the following process steps: fresh small pig intestines are taken as raw materials, intestine casings are peeled off through an intestine scraper to obtain intestinal mucosa, antioxidant is added to protect mucosal protein, and the joint production of crude heparin sodium and active intestinal protein is realized through operations of low salinity enzymolysis, resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like.
The specific process steps are as follows:
s1, intestinal mucosa extraction: taking 100 fresh small intestines of pigs, peeling off the casings by using a intestine scraper to obtain intestinal mucosa, simultaneously washing with purified water, collecting an intestinal mucosa mixed solution 231L, and adding 462g of antioxidant sodium bisulfite into the intestinal mucosa mixed solution to perform antioxidant protection on intestinal proteins.
S2, low-salinity enzymolysis: pumping the intestinal mucosa mixed solution obtained in the step S1 into an enzymolysis tank by a pump, adding 6mol/L sodium hydroxide solution to adjust the pH value range to 7.5, heating and stirring, controlling the temperature to 55 ℃, adding 184g of Novoxil neutral compound type proteolytic enzyme, stirring and carrying out enzymolysis for 5 hours. Adding sodium hydroxide solution to adjust the pH value to 9.5, raising the temperature of the feed liquid to 90 ℃, and continuing stirring for 1 h. Adding concentrated hydrochloric acid into the feed liquid to adjust the pH value to 8.0, and cooling to 55 ℃.
S3, resin adsorption: pumping the enzymatic hydrolysate obtained in the step S2 into an adsorption tank by a pump, adding treated A98 type anion exchange resin, heating to maintain the temperature of the feed liquid at 55 ℃, stirring for 2h until azure A test paper detects that the enzymatic hydrolysate does not show red, opening a filter valve below an adsorption tube, filtering, and collecting the enzymatic hydrolysate. The resin is further washed with purified water in an amount of 3 times the volume of the resin, and the washing solution is collected and combined with the enzymatic hydrolysate.
S4, gradient salinity elution: firstly, preparing 2.0 percent sodium chloride eluent with 3 times of resin volume and 15 percent sodium chloride eluent with 3 times of resin volume, and heating to 55 ℃; firstly, adding 2.0% sodium chloride eluent with the volume 1.5 times that of the resin into an adsorption tank, heating to 55 ℃, stirring for 1h, collecting eluent 1, adding 2.0% sodium chloride eluent with the volume 1.5 times that of the resin into the adsorption tank again, heating to 55 ℃, stirring for 1h, collecting eluent 2, combining eluent 1 and eluent 2, and combining eluent 1, eluent 2 and the enzymolysis liquid obtained in the step S3 to obtain total protein eluent; adding 15% sodium chloride eluent with the volume 1.5 times that of the resin, heating to 55 ℃, stirring for 1h, and collecting eluent 3. Then adding 15% sodium chloride eluent with the volume 1.5 times of the resin volume again, heating to 55 ℃, stirring for 1h, and collecting eluent 4. And combining the eluent 3 and the eluent 4 to obtain the crude heparin sodium eluent.
S5, preparation of active intestinal protein peptide: and (4) adding 2.0 volume of ethanol into the total protein eluent obtained in the step (S4), performing centrifugal separation by a continuous flow centrifuge to obtain an active intestinal protein wet product, then transferring the active intestinal protein wet product into a vacuum reduced pressure drying oven for drying for 3 hours, and crushing to obtain 1330g of active intestinal protein peptide powder.
S6, preparation of crude heparin sodium: and (4) adding 2.0 volume of ethanol into the crude heparin eluent obtained in the step (S4), standing for 5 hours, and removing the supernatant. And (3) adding ethanol into the thick heparin crude product at the bottom for dehydration, collecting powdery crude heparin sodium, transferring the powdery crude heparin sodium into a reduced-pressure vacuum drying oven for drying for 3 hours, and crushing to obtain 103g of crude heparin sodium.
The process flow diagram of this example is shown in figure 1.
The crude heparin, intestinal mucin peptide and production wastewater prepared in this example were tested for salt content, and the test results are shown in tables 4 to 6:
TABLE 4 crude heparin sodium detection results
Inspection item | Internal control standard | The result of the detection |
Potency of the drug | 80-120IU/mg | 93IU/mg |
Loss on drying | ≤10% | 4.2% |
Chloride compound | ≤50ppm | Compliance with regulations |
Heavy metals | ≤30ppm | Compliance with regulations |
pH value | 5.0-7.0 | 6.1 |
Rumination gene | Negative of | Compliance with regulations |
TABLE 5 intestinal mucin peptide assay results
Inspection item | Internal control standard | The result of the detection |
Nitrogen content | 15-20% | 17% |
Loss on drying | ≤10% | 3.4% |
Chloride compound | ≤50ppm | Compliance with regulations |
Heavy metals | ≤30ppm | Compliance with regulations |
pH value | 5.0-7.0 | 6.6 |
Rumination gene | Negative of | Compliance with regulations |
TABLE 6 test results of wastewater
Inspection item | Internal control standard | The result of the detection |
Sodium chloride content | ≤2000ppm | 1620ppm |
Ammonia nitrogen | ≤8 | 6.5 |
COD | ≤60 | 51.6 |
Example 3
A process for separating and coproducing crude heparin sodium and active intestinal protein under the condition of low salt concentration comprises the following process steps: fresh small pig intestines are taken as raw materials, intestine casings are peeled off through an intestine scraper to obtain intestinal mucosa, antioxidant is added to protect mucosal protein, and the joint production of crude heparin sodium and active intestinal protein is realized through operations of low salinity enzymolysis, resin adsorption, gradient salinity elution, alcohol precipitation, drying, crushing and the like.
The specific process steps are as follows:
s1, intestinal mucosa extraction: taking 100 fresh small intestines of pigs, peeling off casings by using a intestine scraper to obtain intestinal mucosa, simultaneously washing with purified water, collecting an intestinal mucosa mixed solution 228L, and adding 342g of antioxidant sodium bisulfite into the intestinal mucosa crushing solution to perform antioxidant protection on intestinal proteins.
S2, low-salinity enzymolysis: pumping the intestinal mucosa mixed solution obtained in the step S1 into an enzymolysis tank by a pump, adding 6mol/L sodium hydroxide solution to adjust the pH value range to 6.8, heating and stirring, controlling the temperature to 48 ℃, adding 160g of Novoxil neutral compound type proteolytic enzyme, stirring and carrying out enzymolysis for 4.5 h. Adding sodium hydroxide solution to adjust the pH value to 8.0, raising the temperature of the feed liquid to 87 ℃, and continuing stirring for 3 hours. Adding concentrated hydrochloric acid into the feed liquid to adjust the pH value to 7.0, and cooling to 53 ℃.
S3, resin adsorption: pumping the enzymolysis liquid obtained in the step S2 into an adsorption tank by a pump, adding treated A98 type anion exchange resin, heating to maintain the temperature of the material liquid at 52 ℃, stirring for 1.5h until azure A test paper detects that the enzymolysis liquid does not show red, opening a filtering valve below an adsorption tube, filtering, and collecting the enzymolysis liquid. The resin is further washed with purified water in an amount of 3 times the volume of the resin, and the washing solution is collected and combined with the enzymatic hydrolysate.
S4, gradient salinity elution: firstly, preparing 2.0% sodium chloride eluent with 3 times of resin volume and 15% sodium chloride eluent with 3 times of resin volume, heating to 52 ℃, firstly adding 2.0% sodium chloride eluent with 1.5 times of resin volume into an adsorption tank, heating to 55 ℃, stirring for 1 hour, collecting eluent 1, adding 2.0% sodium chloride eluent with 1.5 times of resin volume into the adsorption tank again, heating to 55 ℃, stirring for 1 hour, collecting eluent 2, merging eluent 1 and eluent 2, and merging eluent 1, eluent 2 and the enzymolysis liquid obtained in the step S3 to obtain total protein eluent; then adding 15% sodium chloride eluent with the volume 1.5 times of that of the resin into the adsorption tank, heating to 55 ℃, stirring for 1h, and collecting eluent 3. Then adding 15% sodium chloride eluent with the volume 1.5 times of the resin volume again, heating to 55 ℃, stirring for 1h, and collecting eluent 4. And combining the eluent 3 and the eluent 4 to obtain the crude heparin sodium eluent.
S5, preparation of active intestinal protein peptide: and (4) adding ethanol with the volume being 1.8 times that of the feed liquid into the total protein eluent obtained in the step (S4), separating by a continuous flow centrifuge to obtain an active intestinal protein wet product, then transferring the active intestinal protein wet product into a reduced pressure vacuum drying oven for drying for 5 hours, and crushing to obtain 1410g of active intestinal protein peptide powder.
S6, preparation of crude heparin sodium: adding ethanol with the volume 1.8 times that of the crude heparin eluent, standing for 6h, and removing the supernatant. And (3) adding ethanol into the thick heparin crude product at the bottom for dehydration, collecting powdery crude heparin sodium, transferring the powdery crude heparin sodium into a reduced-pressure vacuum drying oven for drying for 5 hours, and crushing to obtain 97g of crude heparin sodium.
The process flow diagram of this example is shown in figure 1.
The crude heparin, intestinal mucin peptide and production wastewater prepared in this example were tested for salt content, and the test results are shown in tables 7 to 9:
TABLE 7 crude heparin sodium assay results
Inspection item | Internal control standard | The result of the detection |
Potency of the drug | 80-120IU/mg | 99IU/mg |
Loss on drying | ≤10% | 3.4% |
Chloride compound | ≤50ppm | Compliance with regulations |
Heavy metals | ≤30ppm | Compliance with regulations |
pH value | 5.0-7.0 | 6.8 |
Rumination gene | Negative of | Compliance with regulations |
TABLE 8 detection results of intestinal mucin peptides
Inspection item | Internal control standard | The result of the detection |
Nitrogen content | 15-20% | 16% |
Loss on drying | ≤10% | 3.9% |
Chloride compound | ≤50ppm | Compliance with regulations |
Heavy metals | ≤30ppm | Compliance with regulations |
pH value | 5.0-7.0 | 6.4 |
Rumination gene | Negative of | Compliance with regulations |
TABLE 9 test results of wastewater
Example 4
Heparin has many negative effects such as bleeding and the induction of thrombocytopenia in long-term use, and low molecular weight heparin is a fraction obtained by separating some components from normal heparin or produced after lysis. The substance has low molecular weight and single structure, the antithrombotic effect of the substance is better than that of heparin, the anticoagulation effect of the substance is lower than that of heparin, and the substance has the characteristics of high bioavailability, long half-life period in vivo, small bleeding tendency, easy absorption by oral administration and the like.
The specific process steps are as follows:
s1, intestinal mucosa extraction: taking 100 fresh small intestines of pigs, peeling off casings by using a intestine scraper to obtain intestinal mucosa, simultaneously washing with purified water, collecting an intestinal mucosa mixed solution 228L, and adding 342g of antioxidant sodium bisulfite into the intestinal mucosa crushing solution to perform antioxidant protection on intestinal proteins.
S2, low-salinity enzymolysis: pumping the intestinal mucosa mixed solution obtained in the step S1 into an enzymolysis tank by a pump, adding 6mol/L sodium hydroxide solution to adjust the pH value range to 6.8, heating and stirring, controlling the temperature to 48 ℃, adding 160g of Novoxil neutral compound type proteolytic enzyme, stirring and carrying out enzymolysis for 4.5 h. Adding sodium hydroxide solution to adjust the pH value to 8.0, raising the temperature of the feed liquid to 87 ℃, and continuing stirring for 3 hours. Adding concentrated hydrochloric acid into the feed liquid to adjust the pH value to 7.0, and cooling to 53 ℃.
S3, resin adsorption: pumping the enzymolysis liquid obtained in the step S2 into an adsorption tank by a pump, adding treated A98 type anion exchange resin, heating to maintain the temperature of the material liquid at 52 ℃, stirring for 1.5h until azure A test paper detects that the enzymolysis liquid does not show red, opening a filtering valve below an adsorption tube, filtering, and collecting the enzymolysis liquid. The resin is further washed with purified water in an amount of 3 times the volume of the resin, and the washing solution is collected and combined with the enzymatic hydrolysate.
S4, gradient salinity elution: firstly, preparing 2.0% sodium chloride eluent with 3 times of resin volume and 15% sodium chloride eluent with 3 times of resin volume, heating to 52 ℃, firstly adding 2.0% sodium chloride eluent with 1.5 times of resin volume into an adsorption tank, heating to 55 ℃, stirring for 1 hour, collecting eluent 1, adding 2.0% sodium chloride eluent with 1.5 times of resin volume into the adsorption tank again, heating to 55 ℃, stirring for 1 hour, collecting eluent 2, merging eluent 1 and eluent 2, and merging eluent 1, eluent 2 and the enzymolysis liquid obtained in the step S3 to obtain total protein eluent; then adding 15% sodium chloride eluent with the volume 1.5 times of that of the resin into the adsorption tank, heating to 55 ℃, stirring for 1h, and collecting eluent 3. Then adding 15% sodium chloride eluent with the volume 1.5 times of the resin volume again, heating to 55 ℃, stirring for 1h, and collecting eluent 4. And combining the eluent 3 and the eluent 4 to obtain the crude heparin sodium eluent.
S5, preparation of active intestinal protein peptide: and (4) adding ethanol with the volume being 1.8 times that of the feed liquid into the total protein eluent obtained in the step (S4), separating by a continuous flow centrifuge to obtain an active intestinal protein wet product, then transferring the active intestinal protein wet product into a reduced pressure vacuum drying oven for drying for 5 hours, and crushing to obtain 1410g of active intestinal protein peptide powder.
S6, preparation of crude heparin sodium: adding ethanol with the volume 1.8 times that of the crude heparin eluent, standing for 6h, and removing the supernatant. And (3) adding ethanol into the thick heparin crude product at the bottom for dehydration, collecting powdery crude heparin sodium, transferring the powdery crude heparin sodium into a reduced-pressure vacuum drying oven for drying for 5 hours, and crushing to obtain 97g of crude heparin sodium.
S7, preparation of low molecular weight heparin sodium: dissolving the crude heparin sodium prepared in the step S6 in 1.5% acetic acid solution, adding sodium nitrite aqueous solution, adjusting the pH value of the solution to 2.5, controlling the temperature to be 25 ℃, stirring for 4h, then adding 4mol/L sodium hydroxide solution to adjust the pH value of the solution to be neutral, raising the temperature to 40 ℃, adding sodium borohydride, reducing the heparin sodium, then adding 6mol/L hydrochloric acid to adjust the pH value of the solution to be 3.5, keeping for 30min, then adding 4mol/L sodium hydroxide solution to adjust the pH value of the solution to be 6.5, adding ethanol with the volume 2.5 times that of the reaction solution, standing for 12h, removing supernatant, centrifuging and collecting precipitate; dissolving the precipitate in sodium chloride aqueous solution, adjusting the pH value of the solution to 2.5 by using 6mol/L hydrochloric acid, keeping the solution for 20min, adding 4mol/L sodium hydroxide solution to adjust the pH value of the solution to 6.5, adding 2.5 times of ethanol, standing for 10h, removing supernatant, centrifuging and collecting the precipitate; dissolving the precipitate in distilled water, separating with chromatographic column, collecting fractions, mixing the fractions with high concentration, ultrafiltering, concentrating, precipitating with ethanol, standing, centrifuging, collecting precipitate, and vacuum drying to obtain low molecular weight heparin sodium.
The detection results of the crude heparin and the intestinal mucosa protein peptide obtained by the embodiment of the invention all accord with the proposed internal control standard, and the product has reliable quality, controllable process and lower production cost; according to the invention, neutral compound protease hydrolysis and alkaline condition hydrolysis are combined to effectively dissociate glycosaminoglycan and protein, so that high-salinity wastewater is avoided, and the discharge of salt-containing nitrogen-containing wastewater is greatly reduced; meanwhile, the antioxidant is added into the mixed liquor of the intestinal mucosa to carry out antioxidant protection on the intestinal mucosa, thus effectively protecting the intestinal mucosa protein and leading the product to have higher economic value. When the low molecular weight heparin sodium is prepared, sodium borohydride is used as a reducing agent to reduce the heparin sodium, hydrochloric acid is added into the solution, then the solution is neutralized by sodium hydroxide solution, and ethanol is precipitated.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A low-salt-concentration heparin sodium and active intestinal protein peptide separation and coproduction process is characterized by comprising the following process steps: fresh small pig intestines are taken as raw materials, intestine casings are peeled off through an intestine scraper to obtain intestinal mucosa, antioxidant is added to protect mucosal protein, and the joint production of crude heparin sodium and active intestinal protein is realized through low salinity enzymolysis, resin adsorption, gradient salinity elution, alcohol precipitation, drying and crushing.
2. The process for separating and co-producing low-salt concentration heparin sodium and active intestinal protein peptide according to claim 1, which is characterized by comprising the following process steps:
s1, extraction of intestinal mucosa: taking fresh small intestine of pig, peeling off the casing by a intestine scraper to obtain intestinal mucosa, washing with purified water, collecting intestinal mucosa mixed solution, and adding antioxidant;
s2, low-salinity enzymolysis: transferring the intestinal mucosa mixed solution obtained in the step S1 to an enzymolysis tank, adding a sodium hydroxide solution to adjust the pH value of the mixed solution to 6.0-7.5, heating and stirring, controlling the temperature of the solution to be 45-55 ℃, adding neutral compound proteolytic enzyme to carry out enzymolysis, adding the sodium hydroxide solution to adjust the pH value of the mixed solution to 8.5-9.5 after the enzymolysis is finished, heating the mixed solution to 85-90 ℃, continuing stirring for 1-3h, adding concentrated hydrochloric acid to adjust the pH value of the mixed solution to 7.0-8.0 after the stirring is finished, and reducing the temperature of the mixed solution to 50-55 ℃;
s3, resin adsorption: transferring the enzymatic hydrolysate obtained in the step S2 to an adsorption tank, adding anion exchange resin, maintaining the temperature of the enzymatic hydrolysate at 50-55 ℃, stirring for 1-2h until azure A test paper detects that the enzymatic hydrolysate does not show red, filtering, collecting the enzymatic hydrolysate, washing the resin with purified water, collecting washing liquid, and combining the washing liquid with the enzymatic hydrolysate;
s4, gradient salinity elution: preparing 2.0% sodium chloride eluate and 15% sodium chloride eluate, and heating to 50-55 deg.C; firstly, adding 2.0% sodium chloride eluent into an adsorption tank, heating and stirring the eluent, collecting eluent 1, adding 2.0% sodium chloride eluent again, heating and stirring the eluent, collecting eluent 2, and merging eluent 1, eluent 2 and the enzymatic hydrolysate obtained in the step S3 to obtain total protein eluent; then adding 15% sodium chloride eluent into the adsorption tank, heating and stirring, collecting eluent 3, adding 15% sodium chloride eluent again, heating and stirring, collecting eluent 4, and combining eluent 3 and eluent 4 to obtain crude heparin sodium eluent;
s5, preparation of active intestinal protein peptide: adding ethanol with the volume of 1.5-2.0 times into the total protein eluent obtained in the step S4, performing centrifugal separation to obtain an active intestinal protein wet product, and drying and crushing to obtain active intestinal protein peptide powder;
s6, preparation of crude heparin sodium: and (4) adding 1.5-2.0 times of ethanol by volume into the crude heparin sodium eluent obtained in the step (S4), standing for 4-6h, removing supernatant, taking the thick heparin crude product at the bottom, adding ethanol for dehydration, collecting powdery crude heparin sodium, drying and crushing to obtain the crude heparin sodium.
3. The process for separating and co-producing heparin sodium with low salt concentration and active intestinal protein peptide according to claim 2, wherein the amount of the antioxidant in step S1 is in the range of 1.0-2.0g/L, and the antioxidant is selected from one or more of sodium metabisulfite, sodium bisulfite and sodium sulfite.
4. The process for separating and co-producing low-salt concentration heparin sodium and active intestinal protein peptide according to claim 2, wherein the concentration of the sodium hydroxide solution in the step S2 is 6mol/L, the addition amount of the neutral compound type proteolytic enzyme is 0.5-0.8g/L, and the enzymolysis time is 4-5 h.
5. The process for separating and co-producing heparin sodium with low salt concentration and active intestinal protein peptide as claimed in claim 2, wherein the resin in step S3 is anion exchange resin A98, and the amount of the purified water is 3 times of the volume of the resin.
6. The process for separating and co-producing heparin sodium with low salt concentration and active intestinal protein peptide according to claim 2, wherein in step S4, the amount of eluent added each time is 1.5 times of the volume of resin, the heating temperature is 55 ℃, and the stirring is carried out for 1 h.
7. The process for separating and co-producing heparin sodium with low salt concentration and active intestinal protein peptide according to claim 2, wherein the drying conditions in step S5 are as follows: drying in a vacuum drying oven under reduced pressure for 3-5 h.
8. The process for separating and co-producing heparin sodium with low salt concentration and active intestinal protein peptide according to claim 2, wherein the drying conditions in step S6 are as follows: drying in a vacuum drying oven under reduced pressure for 3-5 h.
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Denomination of invention: A low salt concentration heparin sodium and active intestinal peptide separation and co production process Effective date of registration: 20231129 Granted publication date: 20220819 Pledgee: Bank of China Limited Huangchuan sub branch Pledgor: HUANGCHUAN PENGSHENG LIVESTOCK PRODUCTS CO.,LTD. Registration number: Y2023980068138 |