CN113980951A - Immobilized CALB preparation method based on nano dialdehyde starch carrier - Google Patents

Immobilized CALB preparation method based on nano dialdehyde starch carrier Download PDF

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CN113980951A
CN113980951A CN202111394017.6A CN202111394017A CN113980951A CN 113980951 A CN113980951 A CN 113980951A CN 202111394017 A CN202111394017 A CN 202111394017A CN 113980951 A CN113980951 A CN 113980951A
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nano
starch
particles
reaction
dialdehyde starch
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于殿宇
董天玉
杜春宇
曲佳瑶
李勋
潘明喆
王立琦
江连洲
刘天一
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Northeast Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

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Abstract

The invention relates to the field of enzyme engineering, and discloses a preparation method of immobilized CALB based on a nano dialdehyde starch carrier. The preparation method of the immobilized enzyme comprises the following steps: firstly, waxy corn starch is used for preparing nano starch particles through a pre-gelatinization method, moisture of the nano starch particles is removed through vacuum drying, and the dried nano starch particles are oxidized through sodium periodate to obtain dialdehyde starch nano particles. Then, preparing the nano Fe by a coprecipitation method3O4The prepared dialdehyde starch nano-particles and the prepared nano Fe3O4And combining to obtain the composite carrier. Then the CALB is fixed on the prepared composite carrier through a cross-linking method to obtain the nano-based composite carrierImmobilization of dialdehyde starch Carrier CALB. And the method has better recovery rate of enzyme activity and higher reusability.

Description

Immobilized CALB preparation method based on nano dialdehyde starch carrier
Technical Field
The invention relates to a preparation method of immobilized enzyme by using nano dialdehyde starch as a carrier, in particular to a method for fixing CALB to nano dialdehyde starch and Fe3O4A method for compounding a carrier.
Background
CALB is widely used in various industrial applications because of its characteristics of high efficiency catalysis, high selectivity, mild reaction conditions, environmental friendliness, etc. However, free CALB has limited application due to its poor stability, inability to be reused, difficulty in separation from the reaction system after reaction, and the like.
The enzyme immobilization technology can effectively solve the above problems. In the immobilization technology, the selection of the carrier material is very important for immobilizing the enzyme. Active groups on the carrier material, microenvironment, shape and size of the carrier and other factors can influence the affinity of the carrier and the enzyme, the activity, stability, reusability and application effect of the immobilized enzyme. Compared with other nanometer materials, the magnetic nanometer material has more advantages in the aspect of preparing immobilized enzyme. The magnetic nano material not only has the advantages of the nano material, but also has the characteristics of superparamagnetism, high magnetic susceptibility and the like, and the immobilized enzyme can be directionally moved by changing a magnetic field. In the process of enzyme immobilization, we generally adopt a cross-linking method. Crosslinking is a method for fixing enzyme by utilizing a bifunctional or multifunctional crosslinking agent to perform a crosslinking reaction between the enzyme and a carrier to form a network structure. Glutaraldehyde is widely used as a cross-linking agent in the process of enzyme immobilization because of its advantages of low cost and good cross-linking effect. But since glutaraldehyde is used in addition to the cross-linking agent, the enzyme is cross-linked to the support. It also invades the active site of the enzyme and forms a rigid pyridine ring, destroying the conformation of the enzyme and inactivating it. And its use is limited due to its toxicity. It has been a trend to select a cross-linking agent that is non-toxic and does not negatively affect the enzyme. The method of modifying the polysaccharide material and endowing aldehyde groups to the polysaccharide material can effectively replace glutaraldehyde as a cross-linking agent to be applied to the immobilization process of the enzyme. Dialdehyde starch is modified starch which is prepared by oxidizing hydroxyl groups on 2-c, 3-c into aldehyde groups by the action of sodium periodate. Dialdehyde starch is widely applied to the cosmetic and pharmaceutical industries due to the characteristics of no toxicity and multiple active aldehyde groups. Therefore, the dialdehyde starch is used for replacing glutaraldehyde, so that the loss of CALB can be effectively reduced, the use efficiency of immobilized enzyme is improved, and the economic loss is reduced.
Disclosure of Invention
The invention aims to provide a preparation method of immobilized enzyme with nano dialdehyde starch as a carrier, aiming at the defects of the existing method, the nano dialdehyde starch is used for replacing glutaraldehyde as a cross-linking agent, so that CALB is effectively immobilized, and simultaneously, the enzyme activity of CALB is more retained.
The invention adopts nano Fe3O4As a carrier core, nano dialdehyde starch as a cross-linking agent, and CALB fixed to Fe3O4A composite carrier as a core. Compared with other immobilization methods, the method has higher enzyme activity retention rate and higher reusability.
The specific implementation mode is as follows:
the first embodiment is as follows:
preparing the nano starch granules. Waxy corn starch was placed in a vacuum oven at 30 ℃ for 2h before reaction. After being taken out, 10g of dried waxy corn starch is weighed and placed in 400mL of deionized water, a rotor with the specification of 4cm is put into a beaker with the specification of 500mL, and a preservative film is covered on the cup mouth to prevent water loss. Heating the mixture in a magnetic water bath at 95 deg.C and 400rpm for 1 hr to completely gelatinize waxy corn starch. After the reaction was completed, the gelatinized waxy corn starch suspension was cooled to room temperature, and the suspension was slowly poured into 2000mL of anhydrous ethanol at 2mL/min and continuously stirred at 600rpm to remove water. After complete washing, separating out the supernatant by using a centrifuge with the rotating speed of 3000rpm, taking out the precipitate, spreading the precipitate in a flat dish, putting the flat dish into a vacuum drying oven at 35 ℃ for drying for 1h, and putting the flat dish into the vacuum drying oven for standby after the drying is finished.
The second embodiment is as follows:
preparing nano dialdehyde starch granules, taking 8g of the prepared nano starch granules, adding the nano starch granules into a flask filled with 100g of water, adding 0.32g of high sodium potassium sulfate, and adding a rotor with the specification of 3 cm. The pH of the suspension was adjusted to 3.5 with 0.5M sulfuric acid and reacted in a water bath at 30 ℃ for 4h at 160rpm in the dark. And after the reaction is finished, alternately washing the reaction product for 3 times by using deionized water and absolute ethyl alcohol, and drying the reaction product for 1h at the temperature of 30 ℃ in a vacuum drying oven for later use after the washing is finished to obtain the nano dialdehyde starch.
The third concrete implementation mode:
5.4g FeCl was precipitated by coprecipitation3·6H2O solid with 1.99g FeCl2·4H2O is mixed in a molar ratio of 2:1 was dissolved in 100mL of ultrapure water and purged with nitrogen continuously. Adding a rotor with the specification of 3cm, rotating at 160rpm, and heating the reaction solution to 70 ℃ in a nitrogen environment after the solution is completely dissolved. Then adding 20mL of ammonia water into the reaction system to prevent reactants from splashing and slowly increase the rotating speed to 270rpm, continuously dropwise adding the ammonia water to adjust the pH value of the reaction system to be more than or equal to 10, heating to 80 ℃ for reaction for 1h, finally cooling the solution to room temperature, and then using a permanent magnet to enable Fe to be generated3O4Separating the nano particles, alternately washing the particles with ethanol and deionized water for 3 times until the particles are neutral, and drying the particles for 2 hours at 55 ℃ in a vacuum drying oven to obtain Fe3O4And (3) nanoparticles.
The fourth concrete implementation mode:
1.0g of nano dialdehyde starch (aldehyde content is determined to be 25.1%) is dissolved in a beaker containing 500mL of ultrapure water, and Fe is added3O42.0g of magnetic nanoparticles and sonicated to disperse them uniformly. After dispersion, 0.2mL of epichlorohydrin is added into the reaction system, a preservative film is covered on the mouth of a beaker to prevent the liquid from evaporating, and the reaction system is shaken in a constant temperature oscillation box at 40 ℃ for 5 hours at the speed of 160 rpm. After the reaction is finished, the mixture is alternately washed three times by absolute ethyl alcohol and deionized water and is placed in a vacuum oven at the temperature of 40 ℃ for drying for 1 hour. To obtain Fe3O4A magnetic core of nano dialdehyde starch coated carrier.
The fifth concrete implementation mode:
the prepared carrier is washed 3 times by 0.1M phosphate buffer solution with pH 7.0 and is placed in the phosphate buffer solution for soaking for 1 hour for standby. 1g of dialdehyde starch nanoparticles was added to 0.1M phosphate buffer pH 7.0200 mL. Then 50mL of CALB enzyme solution with 6% enzyme content was added into the reaction system. The mixed solution was shaken at a certain temperature for a certain period of time in a constant temperature shaking cabinet, the rotation speed of which was 150 rpm. After the reaction, the supernatant was separated by magnetic separation, and the magnetic particles were repeatedly washed with 0.1MpH 7.0.0 phosphate buffer and then with deionized water. And (3) placing the washed product in a vacuum drying oven at 40 ℃ for 4h to obtain the immobilized CALB based on the nano dialdehyde starch carrier.

Claims (1)

1. A preparation method of immobilized CALB based on nano dialdehyde starch carrier is characterized in that the damage to enzyme in the cross-linking process is eliminated and the immobilized CALB has reusability by utilizing the function of nano dialdehyde starch to replace glutaraldehyde in the cross-linking agent of immobilized enzyme, and is realized by the following steps:
the method comprises the following steps: preparing nano starch particles; placing waxy corn starch in a vacuum drying oven at 30 ℃ for 2h before reaction; taking out, weighing 10.0g of dried waxy corn starch, placing the waxy corn starch in 400mL of deionized water, putting a rotor with the specification of 4cm into a beaker with the specification of 500mL, and covering a preservative film on the cup mouth to prevent water loss; heating the mixture in a magnetic water bath kettle at 95 ℃ and the rotation speed of 400rpm for 1h to completely gelatinize the waxy corn starch; after the reaction is finished, cooling the gelatinized waxy corn starch suspension to room temperature, slowly pouring the suspension into 2000mL of absolute ethyl alcohol at a rate of 2mL/min, and continuously stirring at 600rpm to remove water; after complete washing, separating out the supernatant by using a centrifuge with the rotating speed of 3000rpm, taking out the precipitate, spreading the precipitate in a flat dish, putting the flat dish into a vacuum drying oven at 35 ℃ for drying for 1h, and still putting the flat dish into the vacuum drying oven for later use after the drying is finished;
step two: preparing nano dialdehyde starch granules, namely adding 8g of the prepared nano starch granules into a flask filled with 100g of water, adding 0.32g of high-potassium sodium, and adding a rotor with the specification of 3 cm; adjusting the pH value of the suspension to 3.5 by using 0.5M sulfuric acid, and reacting for 4 hours in a water bath at 30 ℃ in a dark environment at the rotating speed of 160 rpm; after the reaction is finished, alternately washing the reaction product for 3 times by using deionized water and absolute ethyl alcohol, and drying the reaction product for 1 hour at the temperature of 30 ℃ in a vacuum drying oven for later use after the washing is finished to obtain nano dialdehyde starch;
step three: 5.4g FeCl was precipitated by coprecipitation3·6H2O solid with 1.99g FeCl2·4H2Dissolving O in 100mL of ultrapure water in a molar ratio of 2:1, and continuously introducing nitrogen to discharge air; adding a rotor with the specification of 3cm, adjusting the rotating speed to 160rpm, and heating the reaction solution to 70 ℃ in a nitrogen environment after the solution is completely dissolved; then adding 20mL of ammonia water into the reaction system, preventing the reactants from splashing, slowly increasing the rotating speed to 270rpm, continuously dropwise adding the ammonia water to adjust the pH value of the reaction system to be more than or equal to 10, and heating to 80 ℃ for reaction for 1 h; finally, after the solution is cooled to room temperature, the permanent magnet is used for leading Fe3O4Separating the nano particles, alternately washing the particles with ethanol and deionized water for 3 times until the particles are neutral, and drying the particles for 2 hours at 55 ℃ in a vacuum drying oven to obtain Fe3O4A nanoparticle;
step four: dissolving 1.0g of nano dialdehyde starch with aldehyde group content of 25.1% in a beaker containing 500mL of ultrapure water, adding Fe3O42.0g of magnetic nano particles are subjected to ultrasonic treatment to be uniformly dispersed; after dispersion, adding 0.2mL of epoxy chloropropane into the reaction system, covering a preservative film on the mouth of a beaker to prevent the liquid from evaporating, and shaking for 5 hours in a constant-temperature oscillation box at 40 ℃ at the speed of 160 rpm; after the reaction is finished, alternately washing the mixture for three times by using absolute ethyl alcohol and deionized water, and drying the mixture for 1 hour in a vacuum oven at the temperature of 40 ℃; to obtain Fe3O4A magnetic core of a carrier coated with nano dialdehyde starch;
step five: washing the prepared carrier for 3 times by using 0.1M phosphoric acid buffer solution with the pH value of 7.0, and soaking the carrier in the phosphoric acid buffer solution for 1 hour for later use; adding 1g of dialdehyde starch nanoparticles into 200mL of 0.1M phosphate buffer solution with the pH value of 7.0; then 50mL of CALB enzyme solution with 6% enzyme content is added into the reaction system; oscillating the mixed solution in a constant-temperature oscillation box at a certain temperature for a period of time, wherein the rotation speed of the oscillation box is 150 rpm; after the reaction is finished, separating supernatant by a magnetic separation method, repeatedly washing the magnetic particles by 0.1M phosphoric acid buffer solution with pH 7.0, and repeatedly washing the surfaces of the magnetic particles by deionized water; and (3) placing the washed product in a vacuum drying oven at 40 ℃ for 4h to obtain the immobilized CALB based on the nano dialdehyde starch carrier.
CN202111394017.6A 2021-11-23 2021-11-23 Immobilized CALB preparation method based on nano dialdehyde starch carrier Pending CN113980951A (en)

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CN102517276A (en) * 2011-12-14 2012-06-27 浙江大学 Method for preparing magnetic nano carrier immobilized aldolase with high substrate tolerance
CN107988198A (en) * 2018-01-14 2018-05-04 浙江工商大学 Magnetic flexible carrier Fe3O4The preparation method and applications of-PDA-DAS
CN109293999A (en) * 2018-09-10 2019-02-01 齐鲁工业大学 The preparation method and products obtained therefrom of a kind of starch nano antibacterial laminated film that capableing of complete biodegradable and application
CN111593045A (en) * 2020-04-16 2020-08-28 东北农业大学 Immobilized magnetoenzyme preparation method of OPO grease in supercritical state
CN113061595A (en) * 2021-03-17 2021-07-02 东北农业大学 Method for immobilizing phospholipase C by using epoxy group modified magnetic chitosan nanoparticles as carrier

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Publication number Priority date Publication date Assignee Title
CN102010525A (en) * 2010-11-18 2011-04-13 华侨大学 Method for preparing superparamagnetic micron starch
CN102517276A (en) * 2011-12-14 2012-06-27 浙江大学 Method for preparing magnetic nano carrier immobilized aldolase with high substrate tolerance
CN107988198A (en) * 2018-01-14 2018-05-04 浙江工商大学 Magnetic flexible carrier Fe3O4The preparation method and applications of-PDA-DAS
CN109293999A (en) * 2018-09-10 2019-02-01 齐鲁工业大学 The preparation method and products obtained therefrom of a kind of starch nano antibacterial laminated film that capableing of complete biodegradable and application
CN111593045A (en) * 2020-04-16 2020-08-28 东北农业大学 Immobilized magnetoenzyme preparation method of OPO grease in supercritical state
CN113061595A (en) * 2021-03-17 2021-07-02 东北农业大学 Method for immobilizing phospholipase C by using epoxy group modified magnetic chitosan nanoparticles as carrier

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