CN113974168A - Composite probiotic composition with anti-allergic effect, application and preparation - Google Patents
Composite probiotic composition with anti-allergic effect, application and preparation Download PDFInfo
- Publication number
- CN113974168A CN113974168A CN202111065534.9A CN202111065534A CN113974168A CN 113974168 A CN113974168 A CN 113974168A CN 202111065534 A CN202111065534 A CN 202111065534A CN 113974168 A CN113974168 A CN 113974168A
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- Prior art keywords
- powder
- lactobacillus
- probiotic composition
- composite probiotic
- parts
- Prior art date
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
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Abstract
The application specifically discloses a composite probiotic composition with an anti-allergic effect, application and a preparation. The composite probiotic composition with the anti-allergic effect comprises 140 parts of galacto-oligosaccharide 100-140 parts, 40-60 parts of orange powder, 8-12 parts of silicon dioxide and 14-20 parts of probiotic powder in parts by weight; the probiotic powder is composed of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus. The composite probiotic composition can be used for preparing foods, health-care products and medicines for improving the anti-allergic capacity. The composite probiotic composition with the anti-allergic effect has the advantages of improving the intestinal flora structure of a human body and improving the anti-allergic capability.
Description
Technical Field
The application relates to the field of functional foods, in particular to a composite probiotic composition with an anti-allergic effect, application and a preparation.
Background
Probiotics are living microorganisms which are colonized in human intestinal tracts and can generate health efficacy, thereby improving the micro-ecological balance of hosts and playing a beneficial role. Among them, Lactobacillus (Lactobacillus) and bifidobacterium (bifidobacterium) are most widely used. As a dietary supplement, stimulation of lactic acid secretion lowers intestinal PH, produces products that contribute to the growth of more beneficial microorganisms and enhances the host immune response to pathogens, promoting and maintaining intestinal flora balance. The probiotic intestinal tract field planting can restore the permeability of intestinal mucosa to normal, enhance the barrier function of the intestinal tract, kill external pathogenic bacteria and sensitizers and avoid infection and allergic diseases.
Research finds that the allergic diseases are caused by reduction of certain probiotics in intestinal tracts, imbalance of intestinal metabolites and the like, so that toxins are accumulated in the intestinal tracts, intestinal mucosa is attacked by harmful microorganisms, and the immune function and the anti-allergic capacity of the allergic diseases are reduced. Therefore, a complex probiotic composition capable of regulating intestinal probiotics and maintaining the balance of intestinal flora is needed.
Disclosure of Invention
In order to adjust intestinal micro-ecological balance, improve anti-allergic capability and improve physique, the application provides a composite probiotic composition with anti-allergic effect, application and a preparation.
In a first aspect, the present application provides a composite probiotic composition with anti-allergic effect, which adopts the following technical scheme:
the composite probiotic composition with the anti-allergic effect comprises 140 parts of galacto-oligosaccharide 100-140 parts, 40-60 parts of orange powder, 8-12 parts of silicon dioxide and 14-20 parts of probiotic powder in parts by weight;
the probiotic powder consists of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus.
Optionally, the weight ratio of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus is (4-6): 3-6): 2-6): 1-2): 1.5-3.5.
According to the technical scheme, the compound probiotic composition is prepared by selecting five specific probiotics and adding the auxiliary agents of galactooligosaccharide, orange powder and silicon dioxide, the prepared compound probiotic composition has the effects of improving the intestinal flora of a human body, regulating the immunity of the organism and improving the anti-allergic capability of the human body, the addition of the galactooligosaccharide can promote the rapid propagation of the probiotics in the body and improve the intestinal colonization capability of the flora, and the silicon dioxide is used as a dispersing agent and can reduce the agglomeration of the compound probiotic composition, so that the flora is propagated in the intestinal tract and better attached to the surface of the intestinal tract after the compound probiotic composition is taken.
Five probiotics cooperations that this application used improve the anti-allergic effect of this application composite probiotic composition to improve user's anti-allergic capacity, improve user's physique, reduce the emergence of allergic condition even avoided the anaphylactic condition.
Preferably, the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is (4-6): 1-1.5): 3-3.5.
Preferably, the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is 5:5:5:1.25: 3.33.
Preferably, the composite probiotic composition further comprises 900 parts by weight of inulin 700-.
By adopting the technical scheme, the dosage proportion of the five probiotics is further optimized, and the inulin is added and combined with the galacto-oligosaccharide, so that the prepared composite probiotic composition is more suitable for the intestines and the stomach of adults, and therefore, a probiotic protection barrier can be quickly established in the intestines and the stomach, and the allergic constitution can be improved.
Preferably, the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is (4-6): 3-4: (2-3): 1.5-1.8): 1.5-3.
Preferably, the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is 5:3.75:2.5:1.87: 1.67.
Preferably, the complex probiotic composition further comprises 900 parts by weight of fructo-oligosaccharide 700-.
Through the technical scheme, the proportion of the five probiotics is adjusted, so that the composite probiotic composition is more in line with the flora structure of the intestinal tract of children less than 12 years old, the intestinal flora condition of the children can be more specifically adjusted, and the total viable count of the five probiotics is adjusted to a certain extent compared with that of adults, so that the digestive system of the children is more suitable for balancing the flora microecology.
The addition of the fructo-oligosaccharide adds the oligosaccharide prebiotics suitable for children on the one hand, and the inventor finds that the fructo-oligosaccharide can be matched with galacto-oligosaccharide to promote the colonization of the probiotics in the intestinal tract after being taken, thereby being beneficial to expanding the number of the probiotics, stabilizing the structural ratio of the probiotics and improving the effectiveness of the composite probiotic composition after being taken.
Children and adults referred to in the present application are specifically: an age greater than 12 years is considered an adult, and an age less than or equal to 12 years is considered a child.
Optionally, the composite probiotic composition is prepared by the following method: and (2) blending fructo-oligosaccharide, galacto-oligosaccharide, orange powder, silicon dioxide, lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus by adopting powder mixing equipment to obtain the composite probiotic composition.
The composite probiotic composition with the anti-allergic effect is prepared by blending the five bacterial powders and other raw materials, and has the advantages of simple operation and convenient production.
Optionally, the powder mixing device comprises a stirring tank, a feeding device for adding various powders into the stirring tank according to a ratio is arranged above the stirring tank, and a powder packaging machine for receiving the mixed powders and quantitatively packaging the powders is arranged below the stirring tank;
a rotary mixing component and a gas-assisted homogenizing component are arranged inside the stirring tank;
the rotary mixing component comprises a vertically arranged stirring shaft, and a plurality of stirring blades are arranged on the stirring shaft;
the gas-assisted homogenizing assembly comprises a spray head arranged in the stirring tank, one end of the spray head is communicated with an external high-pressure gas source, the other end of the spray head faces to powder in the stirring tank, and air flow is sprayed out of the spray head.
Through the technical scheme, when the stirring shaft rotates, the stirring shaft drives the stirring blades to rotate and uniformly mix the powder in the stirring tank; when the gas-assisted material homogenizing assembly works, the spray head blows airflow into the stirring tank, so that powder deposited below the stirring tank is blown up. When the rotary mixing component and the gas-assisted homogenizing component work together, the powder blown up by the airflow of the gas-assisted homogenizing component participates in the mixing process of the rotary mixing component again, so that the mixing efficiency is improved, and the mixing uniformity of various kinds of powder in the same batch is improved.
The same batch refers to a batch of products which are mixed by powder mixing equipment after being fed at the same time and are separately packaged in different packaging bags.
Preferably, the gas-assisted homogenizing assembly further comprises a bottom plate, the bottom plate is arranged close to the bottom wall of the stirring tank and is rotatably connected with the stirring tank around the axis of the stirring shaft, and the bottom plate obtains kinetic energy from the stirring shaft through a transmission structure and rotates around the axis of the stirring shaft. The shower nozzle is located the bottom plate below to the gas outlet of shower nozzle sets up towards the bottom plate, and pastes with the bottom plate and lean on. A plurality of air vents are formed in the bottom plate, when the bottom plate rotates, the air vents on the bottom plate are intermittently communicated with the spray head, so that the spray head can spray air intermittently, and the uniformity of material mixing is further improved through an intermittent air spraying mode.
The transmission structure can be further selected as a planetary gear reducer, so that the rotating stability of the bottom plate is improved.
Through the mutual cooperation of the rotary mixing component and the gas-assisted material homogenizing component, on one hand, the mixing speed of the powder materials is increased, and on the other hand, the mixing uniformity of the powder materials is improved.
When the powder mixing equipment is used for producing the composite probiotic composition with the anti-allergic effect, the rotating speed of the rotating and mixing component is 40-90r/min as a preferable scheme, and the pulse period of the pulse airflow is 2-5 s.
More preferably: the rotating speed of the rotating mixing assembly was 60r/min and the pulse period was 3 s.
The agitator tank top still is provided with feeding mechanism, and feeding mechanism includes a plurality of installation roof beams that communicate each other with the conveying pipe, is provided with a plurality of storage hoppers that are used for storing various raw materialss respectively on the installation roof beam, the inside cavity of installation roof beam to a solenoid valve is installed to the storage hopper below, and solenoid valve and the inside intercommunication of storage hopper still are provided with the screw feeder in the installation roof beam.
The powder conveyer belt is arranged below the spiral feeder, the powder conveyer belt and the spiral feeder are separated by two turning plates, and the two turning plates are respectively hinged with the inner wall of the corresponding side in the mounting beam.
Still be connected with a sweep-air pipe on the installation roof beam, sweep the setting of tuber pipe air-out direction towards the conveying pipe.
Through above-mentioned technical scheme, when using the composite probiotic composition that a powder mixing apparatus production this application has anti-allergic efficiency that this application discloses, at first add each raw materials respectively according to the ratio in corresponding ejection of compact jar, then start powder mixing apparatus, the solenoid valve is opened, the powder falls into the spiral feeding district, the work of spiral feeder this moment and send into the conveying pipe with the powder, turn over the board and open after that, the scavenging duct is blown to installation roof beam inside, be stained with the powder that attaches on spiral feeder and fall on the powder conveyer belt and follow the powder conveyer belt and get into the conveying pipe.
After the powder enters the stirring barrel, the driving motor works and drives the stirring shaft and the stirring blade fixedly connected to the stirring shaft to rotate, and the stirring blade stirs the powder in the stirring barrel to promote the uniform mixing of the powder.
The stirring shaft drives the bottom plate to rotate through the reduction gear set, so that air flow is ejected from the nozzle below the bottom plate in a clearance manner, and powder deposited on the bottom plate is blown. Thereby accelerating the mixing speed of the powder and improving the uniformity of the powder mixing.
After the powder mixes the completion, the agitator rotates to powder entering material receiving pipe in the agitator, at the in-process of carrying out the blowing, the air current that blows off to the feed cylinder direction that the air-assisted homocline subassembly can also last, thereby the entering material receiving pipe that makes the powder in the agitator can be more smooth has improved the stability of unloading.
In a second aspect, the present application provides a use of a complex probiotic composition with anti-allergic effect in the preparation of a food or a drug or a health product with anti-allergic effect.
In a third aspect, the present application provides a formulation, using the following technical scheme:
a preparation comprises the composite probiotic composition.
Optionally, the form of the preparation is selected from one of powder, tablet, pill and capsule.
Preferably, the composite probiotic composition can be taken in the form of powder by brewing with warm water below 40 ℃.
In summary, the present application has the following beneficial effects:
1. the composite probiotic composition with the anti-allergic effect is prepared by selecting five specific probiotics and adding galacto-oligosaccharide, orange powder and silicon dioxide. The composite probiotic composition has the advantages of regulating the intestinal flora structure of a human body, reducing the occurrence probability of allergic diseases and improving the anti-allergic capability.
2. This application is through adjusting the probiotic ratio, and further still made the adult type that is more suitable for adult to take and the children type that is more suitable for children to take, and the crowd of different ages can select to take corresponding compound probiotic composition to improvement self physique that can be better improves the antianaphylaxis ability.
3. The disclosed compound probiotic composition of this application mixes through a powder mixing apparatus, and the ratio is more accurate between each material to the dispersibility between the material is good, and after the partial shipment package, the proportion between the probiotic and the raw materials is more accurate in every bag, helps guaranteeing the pharmacodynamic stability of compound probiotic composition. And the mixing speed is accelerated, and the production efficiency is improved.
Drawings
FIG. 1 is a schematic diagram of the overall construction of a powder mixing apparatus for preparing a composite probiotic composition with anti-allergic effect according to the present application;
FIG. 2 is a front view of the powder mixing apparatus;
FIG. 3 is an enlarged view of detail A of FIG. 1, and shows the closure assembly in place;
FIG. 4 is an exploded view of the stirring shaft along its axis, showing the internal structure of the stirring tank;
FIG. 5 is a schematic view showing the internal structure of the feeding mechanism;
FIG. 6 is a cross-sectional view taken along line C-C of FIG. 2;
fig. 7 is an enlarged view of detail B in fig. 1.
Description of reference numerals: 1. a frame body; 11. a stirring tank; 111. a charging barrel; 112. a feed pipe; 113. a material receiving pipe; 12. a rotating shaft; 2. a feeding mechanism; 21. a material storage tank; 22. an electromagnetic valve; 3. rotating the mixing assembly; 31. a drive motor; 32. a stirring shaft; 33. a stirring blade; 4. a gas-assisted homogenizing assembly; 41. a base plate; 411. a connecting pipe; 412. a vent hole; 42. a reduction gear set; 421. a ring gear; 422. a sun gear; 423. a planet wheel; 43. pipe distribution; 44. a header pipe; 45. a spray head; 46. A seal ring; 47. a fixing plate; 471. mounting holes; 5. a sealing assembly; 51. a cover plate; 52. an ear plate; 53. a slide rail; 54. A return spring; 6. a swing mechanism; 61. a control panel; 62. a control shaft; 63. a cylinder; 64. a stabilizing assembly; 65. positioning a plate; 66. positioning a rod; 67. an extension spring; 7. mounting a beam; 71. a support frame; 72. a spiral feeding zone; 73. a conveyor belt feeding area; 74. turning over a plate; 741. a rotating gear; 742. a drive gear; 743. turning over a motor; 75. a screw feeder; 751. a spiral feeding motor; 752. a helical feeding blade; 76. a powder conveyor belt; 77. a wind sweeping pipe; 78. a scraper.
Detailed Description
The bacterial powder used in the present application is:
the Lactobacillus reuteri is specifically Lactobacillus reuteri LE16, the viable count is 1000 hundred million CFU/g, and the Lactobacillus reuteri is purchased from Songjiang division of Shanghai Bingda Onconli GmbH;
the Lactobacillus acidophilus is specifically Lactobacillus acidophilus NCFM, has viable count of 2000 hundred million CFU/g, and is purchased from Danisco (China) Co., Ltd;
the Bifidobacterium lactis is specifically Bifidobacterium lactis Bi-07, has a viable count of 3000 hundred million CFU/g, and is purchased from Danisco (China) Co., Ltd;
the lactobacillus paracasei is specifically lactobacillus paracasei Lpc-37, the viable count is 4000 hundred million CFU/g, and the lactobacillus paracasei is purchased from Danisco (China) Co., Ltd;
the Lactobacillus rhamnosus is specifically Lactobacillus rhamnosus HN001, has a viable count of 4500 hundred million CFU/g, and is purchased from Danisco (China) Co.
The galactooligosaccharides used in the examples of the present application were purchased from quantum gaku (guangdong) bio ltd;
orange powder was purchased from Tongtai flourishing (Beijing) Komao Co;
silica was purchased from Beijing Hengsheng pharmaceutical Co., Ltd;
inulin was purchased from medium Baixing food science and technology (Beijing) Co., Ltd;
fructooligosaccharides were purchased from good health science and technology (shanghai) ltd.
The application provides a preparation method with an antiallergic effect, wherein five kinds of bacterial powder and other raw materials are added into powder mixing equipment to be mixed, and then the composite probiotic composition is obtained.
The powder mixing apparatus of the present application is described in further detail below with reference to FIGS. 1-7.
The utility model provides a powder mixing equipment, refers to fig. 1, includes support body 1, is provided with an agitator tank 11 on the support body 1, and the upper end of agitator tank 11 is provided with the feeding mechanism 2 that is used for carrying the powder in the agitator tank 11, and feeding mechanism 2 top is provided with a plurality of storage tanks 21.
As shown in fig. 1 and 2, the two frame bodies 1 are arranged in parallel, the stirring tank 11 is arranged between the two frame bodies 1, and the stirring tank 11 is supported on the ground through the two frame bodies 1. Specifically, the equal level in 11 both sides lateral walls that deviate from mutually of agitator tank is provided with a rotation axis 12, and two rotation axes 12 are coaxial setting. The two rotating shafts 12 are respectively inserted into the corresponding frame bodies 1 and rotatably connected with the frame bodies 1, so that the stirring tank 11 can rotate around the axes of the rotating shafts 12.
As shown in FIGS. 1 and 2, a cylinder 111 is provided at the top end of the agitation tank 11, the cylinder 111 is communicated with the inside of the agitation tank 11, and the powder can be introduced into the inside of the agitation tank 11 through the cylinder 111. After the powder is mixed in the stirring barrel, the stirring tank 11 can rotate around the stirring shaft 32, so that the charging barrel 111 rotates to the position below the stirring shaft 32 to finish the discharging work.
As shown in fig. 1 and fig. 2, a feeding pipe 112 is disposed above the stirring tank 11 for receiving the powder conveyed by the feeding mechanism 2 and transferring the powder into the stirring tank 11 through a charging barrel 111, a receiving pipe 113 is disposed below the stirring tank 11, and a powder packing machine is connected to the lower end of the receiving pipe 113. After the powder in the stirring tank 11 is blended, the stirring tank 11 rotates around the rotating shaft 12, so that the charging barrel 111 is communicated with the material receiving pipe 113, the powder enters the powder packaging machine below through the material receiving pipe 113, and the powder is quantitatively packaged in the powder packaging machine. The quantitative packaging of the powder packaging machine is known to the person skilled in the art and will not be described further here. The powder packaging machine used in the embodiment of the application is a full-automatic back-sealing powder packaging machine (model WY-300ZF) produced by Qingdao Yuande packaging machinery Co.
As shown in fig. 1 and fig. 3, in order to reduce the situation that the powder in the stirring tank 11 is mixed during stirring and spills when the stirring tank 11 is turned over, a sealing component 5 is further disposed at the charging barrel 111 of the stirring tank 11, the sealing component 5 includes a cover plate 51 disposed at the charging barrel 111, two opposite side walls of the cover plate 51 are respectively and vertically fixedly connected with an ear plate 52, the ear plate 52 is disposed toward the stirring tank 11, one end of the stirring tank 11 fixedly connected with the charging barrel 111 is further provided with two slide rails 53, the two slide rails 53 are disposed in parallel, and the two ear plates 52 are respectively connected with the corresponding slide rails 53 in a sliding manner. The sliding track of the ear plate 52 on the slide rail 53 is an arc, and the center of curvature of the arc is located on the axis of the rotating shaft 12. The end surface of the stirring tank 11 is further provided with two return springs 54, and the two return springs 54 are respectively located on two opposite sides of the two lug plates 52. One end of the return spring 54 is located in the middle of the slide rail 53 and is fixedly connected with the end surface of the stirring tank 11, and the other end of the return spring 54 is fixedly connected with the lug plate 52.
When the stirring tank 11 performs the feeding operation, firstly, the stirring tank 11 rotates, the charging barrel 111 gradually communicates with the feeding pipe 112, the outer circumferential surface of the feeding pipe 112 abuts against the cover plate 51 and pushes the cover plate 51 to slide along the slide rail 53, so that the cover plate 51 leaves the port of the charging barrel 111, and at this time, the return spring 54 is stretched. When the stirring tank 11 rotates and the material cylinder 111 leaves the feeding pipe 112, the cover plate 51 slides back to the opening of the material cylinder 111 under the action of the pulling force of the return spring 54, and the cover plate 51 closes the opening of the material cylinder 111 again, so that the condition that powder is spilled is reduced.
When the stirring tank 11 rotates and the material barrel 111 is communicated with the material receiving pipe 113, the material barrel 111 is opened by the same principle, and when the material barrel 111 rotates away from the material receiving pipe 113, the cover plate 51 covers the material barrel 111 again, and the specific working mode can refer to the butt joint of the material barrel 111 and the material feeding pipe 112, which is not described herein again.
As shown in fig. 1 and fig. 2, a frame body 1 on one side of the stirring tank 11 is further provided with a swing mechanism 6 for driving the stirring tank 11 to rotate around the axis of the rotating shaft 12, the swing mechanism 6 includes a control panel 61 coaxially and fixedly connected to the rotating shaft 12, a control shaft 62 is fixedly connected to the position of the control panel 61 deviating from the center of the circle, and the axis of the control shaft 62 is parallel to the axis of the control panel 61. An air cylinder 63 is arranged on the control shaft 62, a piston rod of the air cylinder 63 is rotatably connected with the control shaft 62, and a cylinder body of the air cylinder 63 is hinged with the frame body 1. When the cylinder 63 works, the piston rod of the cylinder 63 extends out, so that the cylinder 63 drives the control disc 61 to rotate, when the elongation of the cylinder 63 reaches the maximum, the cylinder 63 drives the rotating disc to rotate 180 degrees, so that the stirring tank 11 is controlled to turn over 180 degrees, the charging barrel 111 rotates to a state communicated with the material receiving pipe 113 from a state communicated with the material feeding pipe 112, and then powder is discharged through the material receiving pipe 113. When the stirring tank 11 needs to be loaded again, the air cylinder 63 works, the piston rod of the air cylinder 63 is retracted, the stirring tank 11 rotates in the opposite direction, and the charging barrel 111 rotates to be in butt joint with the feeding pipe 112, so that the stirring tank 11 is convenient to perform subsequent loading work. Specifically, the pin joint of the cylinder 63 and the frame body 1 is located under the control panel 61, so that the rotating stability of the stirring tank 11 is improved.
As shown in fig. 1 and 2, a stabilizing assembly 64 is further disposed on the frame body 1 on one side of the stirring tank 11 departing from the swing mechanism 6, the stabilizing assembly 64 includes a positioning plate 65, the positioning plate 65 is coaxially and fixedly connected to the rotating shaft 12, a positioning rod 66 is further disposed at the position of the center of the circle deviated from the positioning plate 65, an extension spring 67 is sleeved on the positioning rod 66, the other end of the extension spring 67 is located under the center of the circle of the positioning plate 65, and one end of the extension spring 67, which is far away from the positioning plate 65, is fixedly connected to the frame body 1. When the swing mechanism 6 works and the control shaft 62 is located at the lowest point of the motion track of the control shaft, the height of the positioning rod 66 is equal to the height of the circle center of the positioning disc 65. At this time, the opening of the charging barrel 111 on the stirring tank 11 is vertically arranged upwards and communicated with the feeding pipe 112, and similarly, when the control shaft 62 is located at the highest point of the motion track of the control shaft, the positioning rod 66 rotates to the other side of the circle center of the positioning disk 65, the circle centers of the positioning rod 66 and the positioning disk 65 are equal in height, and the opening of the charging barrel 111 is vertically arranged downwards and communicated with the material receiving pipe 113. The extension length of the extension spring 67 is longest in the two states, and the agitator tank 11 has a tendency of rotating in the opposite direction by the tension of the extension spring 67, so that the agitator tank 11 can rotate in the opposite direction to the state that the charging barrel 111 is upward after discharging is completed, and the working stability of the charging barrel 111 is improved.
As shown in fig. 4, a rotary mixing component 3 and an auxiliary gas homogenizing component 4 are arranged in the stirring tank 11 for uniformly mixing the materials. The powder is uniformly mixed under the combined action of the rotary mixing component 3 and the gas auxiliary material homogenizing component 4. Rotatory mixed subassembly 3 includes that the rigid coupling deviates from the driving motor 31 of feed cylinder 111 one end at agitator tank 11, and inside driving motor 31's the vertical ascending agitator tank 11 that penetrates of output shaft, 11 inside vertical (mixing) shafts 32 that are provided with of agitator tank, the coaxial rigid coupling of (mixing) shafts 32 and driving motor 31's output shaft, it has a plurality of stirring vane 33 to prop up along (mixing) shaft 32 axis on the (mixing) shaft 32. When the driving motor 31 works, the driving motor 31 drives the stirring shaft 32 to rotate, so that the stirring blades 33 on the stirring shaft 32 stir the powder, and the powder is fully and uniformly mixed. The stirring blades 33 are arranged obliquely, so that when the stirring shaft 32 rotates, the stirring blades 33 have the effect of stirring the powder upwards along the axial direction of the stirring shaft 32, and the uniformity of powder mixing is further improved.
As shown in fig. 4, the gas-assisted homogenizing assembly 4 includes a bottom plate 41 disposed in the stirring tank 11, the bottom plate 41 is sleeved outside the stirring shaft 32 and can rotate in the stirring tank 11, and a gap is left between the bottom plate 41 and the bottom wall of the stirring tank 11, so that the bottom plate 41 divides the stirring tank 11 into a powder mixing space above and a gas-assisted working space below the bottom plate 41. The lower surface of the bottom plate 41 is provided with a reduction gear set 42 for providing power for the rotation of the bottom plate 41, the reduction gear set 42 comprises a gear ring 421 coaxially sleeved outside the stirring shaft 32, a sun gear 422 fixedly connected on the circumferential surface of the stirring shaft 32, and a planet gear 423 arranged between the gear ring 421 and the sun gear 422, and the planet gear 423 is rotatably connected on the inner bottom wall of the stirring tank 11. The planetary gear 423 meshes with the outer peripheral surface of the sun gear 422 and the inner peripheral surface of the ring gear 421. One end of the gear ring 421 facing the bottom plate 41 is further provided with a connecting pipe 411, splines are arranged at two ends of the connecting pipe 411, two ends of the connecting pipe 411 are respectively connected with the gear ring 421 and the bottom plate 41 through the splines, and therefore the bottom plate 41 can synchronously rotate with the gear ring 421 through the connecting pipe 411. When the driving motor 31 works, the driving motor 31 drives the stirring shaft 32 to rotate, and then the sun gear 422 fixedly connected to the stirring shaft 32 rotates through conduction of the planet gear 423, so that the bottom plate 41 rotates around the axis of the stirring shaft 32. A fixing plate 47 is disposed below the bottom plate 41, the fixing plate 47 is a circular plate, the fixing plate 47 is sleeved on the periphery of the connecting pipe 411, and the fixing plate 47 is fixedly connected to the inner wall of the stirring tank 11. The upper surface of the fixing plate 47 abuts against the lower surface of the bottom plate 41.
As shown in FIG. 4, a plurality of branch pipes 43 are arranged in the gas auxiliary working space along the radial direction of the bottom plate 41, and the plurality of branch pipes 43 are uniformly distributed around the axis of the stirring shaft 32. The end of the branch pipe 43 away from the stirring shaft 32 is provided with an annular main pipe 44, and the main pipe 44 is simultaneously communicated with a plurality of branch pipes 43. An air supply pipe (not shown) is also connected to the manifold 44, and extends out of the stirring tank 11 and is connected to a high-pressure air source (not shown), which may be a high-pressure nitrogen cylinder or high-pressure air supplied by an air pump. Divide pipe 43 to close mouthful setting towards the one end of (mixing) shaft 32, divide pipe 43 to go up along the length direction evenly distributed who divides pipe 43 and have a plurality of shower nozzles 45, the one end and the branch pipe 43 intercommunication of shower nozzle 45, the other end sets up towards bottom plate 41, has seted up a plurality of air vents 412 on the corresponding bottom plate 41, air vent 412 divide into a plurality of groups. A plurality of groups of vent holes 412 are distributed on the floor in a circumferential interval mode around the axis of the stirring shaft 32. The vent holes 412 in each group are distributed at intervals in the radial direction of the bottom plate 41, and the distribution intervals of the vent holes 412 are equal to the distribution intervals of the shower heads 45. A plurality of mounting holes 471 matched with the vent holes 41 are correspondingly formed in the fixing plate 47, one end of the spray head 45 facing the bottom plate 41 extends into the mounting holes 471, a sealing ring 451 is further arranged on the outer peripheral surface of the spray head 45, and the sealing ring 451 is located in the mounting holes 471 and is abutted and sealed with the mounting holes 471. When the bottom plate 41 rotates, the intermittent mounting holes 471 of the vent holes 412 on the bottom plate 41 are communicated, so that the spray heads 45 positioned in the mounting holes 471 intermittently spray air to the powder mixing space.
When the rotary mixing assembly 3 is operated, the bottom plate 41 rotates synchronously with the stirring shaft 32 by the transmission action of the reduction gear set 42, and the rotating speed of the bottom plate 41 is lower than that of the stirring shaft 32, and the rotating direction of the bottom plate 41 is opposite to that of the stirring shaft 32. With the rotation of the bottom plate 41, when the vent holes 412 are aligned with the spray head 45, the air flow blows into the powder mixing space from the vent holes 412 and blows up the powder deposited on the bottom plate 41 so that the powder with the bottom settled by stirring can participate in the stirring again, thereby improving the uniformity of the mixing among the powders. The airflow in the vent 412 is generated in a pulse manner along with the rotation of the bottom plate 41, so as to perform pulse type vibration on the powder in the powder mixing space.
When the powder mixing equipment is used for producing the composite probiotic composition with the anti-allergic effect, the rotating speed of the rotating mixing component 3 is 40-90r/min, the pulse period of the pulse airflow is 2-5s, the preferred rotating speed is 60r/min, and the pulse period is 3 s.
The mode of pulse type airflow vibration powder is selected, so that the uniformity of powder mixing is improved, and the condition that part of powder cannot be uniformly stirred due to bottom sinking is reduced. On the other hand, compared with continuous airflow, the pulse airflow can fall under the action of gravity in the gap of twice airflows after being blown by the pulse airflow, so that the uniform mixing of the powder in the height direction is more facilitated, and the situation that the powder with different weights is layered under the action of self gravity when being blown by the continuous airflow, and the mixing uniformity of the powder is further influenced is reduced.
The spray head 45 is integrally bent into a U shape, the lowest point of the U shape is lower than the connection point of the spray head 45 and the branch pipe 43, the spray head 45 is U-shaped, a small amount of powder entering the spray head 45 is collected at the lowest point of the U shape, and the powder accumulated in the spray head 45 is blown out of the spray head 45 along with the next pulse airflow, so that the powder entering the branch pipe 43 is reduced, the condition of blocking the branch pipe 43 is avoided, and the working stability of the air-assisted material homogenizing assembly 4 is improved.
As shown in fig. 2 and 5, the feeding mechanism 2 is located above the feeding pipe 112, the feeding mechanism includes a plurality of horizontally disposed mounting beams 7, and the mounting beams 7 are radially and uniformly distributed with the top end of the feeding pipe 112 as a starting point. A plurality of storage tanks 21 are arranged on each mounting beam 7 along the length direction of the mounting beam 7. The lower end of the storage tank 21 is communicated with an electromagnetic valve 22, and one end, far away from the storage tank 21, of the electromagnetic valve 22 is communicated with the inside of the mounting beam 7. The powder can fall into the mounting beam from the storage tank 21 through the electromagnetic valve 22 and further enter the stirring tank 11 to participate in powder mixing.
For convenience of description, the following description will be given by taking two mounting beams 7 and a form in which two storage tanks 21 are provided on each mounting beam 7 as an example (for the blending of 8 or 9 raw materials in the present application, the number of the storage tanks 21 and the mounting beams 7 may be adjusted as the case may be, and for example, a form in which 3 mounting beams 7 are provided and 3 storage tanks 21 are provided on each mounting amount may be adopted).
As shown in fig. 5 and 6, a support frame 71 is fixed below the installation beam 7, and the support frame 71 stably supports the installation beam 7 on the ground. The mounting beam 7 is hollow and comprises a spiral feeding area 72 positioned above and a conveyor belt feeding area 73 positioned below, two turning plates 74 are arranged between the spiral feeding area 72 and the conveyor belt feeding area 73, and the turning plates 74 are hinged with the inner wall of the mounting beam 7 on the corresponding side respectively. The spiral feed area 72 and the belt feed area 73 are separated by a flap 74. The spiral feeding area 72 is further provided with a spiral feeding machine 75, the spiral feeding machine 75 includes a spiral feeding blade 752 penetrating through the spiral feeding area 72 and a spiral feeding motor 751 fixedly connected to the outer side of the mounting beam 7 for driving the spiral feeding blade 752 to rotate, and the spiral feeding motor 751 drives the spiral feeding blade 752 to rotate through a belt transmission mode (in other embodiments, a transmission mode such as a gear or a chain can be adopted, and a direct drive mode of the spiral feeding motor 751 can also be adopted). When the two flaps 74 are completely closed, the cross section of the spiral feeding area 72 is circular, and the outer side edge of the spiral feeding blade 752 abuts against the inner wall of the spiral feeding area 72. The powder is pushed into the feed pipe 112 by the rotation of the screw feed blade 752 and falls into the agitation tank 11.
As shown in fig. 1, after the feeding of the powder by the screw feeder 75 is completed, a part of the powder is hung on the side wall of the screw feeding area 72 and the surface of the screw feeding blade 752, and in order to make the part of the powder fall into the stirring tank 11 as much as possible, the accuracy of the distribution ratio of the powder is improved. A scavenging duct 77 is further communicated with the spiral feeding area 72, and the opening direction of the scavenging duct 77 is arranged along the tangential direction of the spiral feeding blade 752. When the air scavenging duct 77 is operated, the air scavenging duct 77 blows an air flow in a tangential direction of the spiral feeding blade 752, thereby purging the inner space of the spiral feeding area 72 and blowing the powder into the feeding pipe 112.
As shown in FIG. 5, a powder conveyor belt 76 is further arranged in the belt feeding area 73, the conveying direction of the powder conveyor belt 76 is arranged towards the direction of the feeding pipe 112, and when the air scavenging pipe 77 works, the turning plate 74 opens a small amount of powder to fall on the surface of the powder conveyor belt 76 and is fed into the feeding pipe 112 along with the powder conveyor belt 76.
The air scavenging pipe 77 is arranged so that the powder remaining in the spiral feeding area 72 is driven by the air flow and blown into the feeding pipe 112, and a small amount of powder falls on the surface of the powder conveyor belt 76 and is conveyed again. The mutual cooperation of two kinds of pay-off modes of screw feeder 75 and powder conveyer belt 76 has improved conveying efficiency and transport precision, and a large amount of powder can be transported through screw feeder 75 in the feeding stage earlier stage, and a small amount of powder in later stage can be carried through the conveyer belt. When the powder conveying belt 76 conveys a large amount of powder, the condition that the conveying belt is deformed due to the weight of the powder, so that the conveying efficiency is influenced is reduced, the condition that the powder is scattered on the outer side of the conveying belt due to the fact that the powder is accumulated on the surface of the conveying belt is reduced, and the powder conveying stability is improved.
As shown in fig. 1 and fig. 7, the turning plate 74 can be opened by a cylinder 63 connecting rod, a motor drive, etc., in this application, a rotation gear 741 is disposed at an end of the turning plate 74, the rotation gear 741 is coaxially and fixedly connected with a hinge shaft of the turning plate 74, a driving gear 742 is simultaneously engaged with the rotation gears 741 of the two turning plates 74, a turning motor 743 is further fixedly connected to an end of the mounting beam 7, and an output shaft of the turning motor 743 is coaxially and fixedly connected with the driving gear 742. When the turnover motor 743 works, the driving gear 742 rotates, the two turnover plates 74 are opened through the meshing of the driving gear 742 and the rotating gear 741, the cambered surfaces of the turnover plates 74 play a role in guiding the falling of the powder, the powder can fall on the middle of the conveying belt in a centralized mode, and therefore the stability of the conveying belt for conveying the powder is improved.
As shown in fig. 5, a scraper 78 is further disposed at one end of the conveyor belt facing the feeding pipe 112, the scraper 78 is fixedly connected to the side wall of the mounting beam 7 in the feeding area 73 of the conveyor belt, and one side of the scraper 78 abuts against the surface of the conveyor belt, so as to scrape the powder attached to the surface of the conveyor belt into the feeding pipe 112, thereby further improving the feeding accuracy.
Examples
Example 1
40016.5g of resistant dextrin, 6000g of galacto-oligosaccharide, 2500g of orange powder, 500g of silicon dioxide, 250g of lactobacillus reuteri, 250g of lactobacillus acidophilus, 250g of bifidobacterium lactis, 100g of lactobacillus paracasei and 133.5g of lactobacillus rhamnosus are weighed. The raw materials are respectively added into each storage tank, the powder mixing equipment is started, and the electromagnetic valves respectively control the corresponding storage tanks to discharge materials. The raw materials are conveyed into a stirring tank to be stirred under the combined action of a spiral feeder and a powder conveying belt, after stirring is finished, the powder is conveyed into a powder packaging machine to be packaged, 2 g/bag of composite probiotic composition is obtained, and the viable bacteria content of each bag of composite probiotic composition is 100 hundred million CFU.
When the powder is mixed in the stirring barrel, the rotating speed of the rotating mixing component is 60r/min, and the pulse period of the pulse airflow blown out by the gas-assisted material homogenizing component is 3 s. Stirring for 120 min.
Theoretically, each bag after subpackaging comprises 1.601g of resistant dextrin, 0.24g of galacto-oligosaccharide, 0.1g of orange powder, 0.02g of silicon dioxide, 0.01g (10 hundred million CFU) of lactobacillus reuteri, 0.01g (20 hundred million CFU) of lactobacillus acidophilus, 0.01g (30 hundred million CFU) of bifidobacterium lactis, 0.004g (16 hundred million CFU) of lactobacillus paracasei and 0.005g (24 hundred million CFU) of lactobacillus rhamnosus.
Examples 2 and 3
The difference from the embodiment 1 is that the rotating speed of the rotating mixing component is different, the pulse period of the pulse airflow blown by the gas auxiliary homogenizing component is different, and the detailed process parameters are shown in the table 1.
Comparative example 1
The difference with the embodiment 1 is that in the comparative example, when the powder is blended in the stirring tank, the gas-assisted homogenizing assembly does not work, the specific bottom plate does not rotate, the vent hole in the bottom plate is not communicated with the spray head, and the gas-assisted homogenizing assembly does not blow air flow into the powder mixing space. The rotating mixing assembly rotates at a speed of 60 r/min.
When the control bottom plate does not rotate, the staff can demolish the planet wheel of planetary gear reducer to interrupt planetary gear reducer's transmission, the bottom plate is in quiescent condition.
Comparative example 2
The difference from the example 1 is that in the comparative example, when the powder is blended in the stirring tank, the bottom plate does not rotate, and the vent hole on the bottom plate is communicated with the spray head. The air auxiliary material homogenizing component continuously blows air flow, and the rotating speed of the rotating mixing component is 60 r/min.
Table 1: examples1-3 and comparative examples 1-2
| Process conditions | Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 |
| Rotational speed (rpm) | 60 | 90 | 40 | 60 | 60 |
| Pulse period(s) | 3 | 2 | 4.5 | ∞ | 0 |
Note that: infinity represents no pulsed air flow blow; 0 represents a continuous pulsed air stream blowing.
Detecting the mixing uniformity of powder:
because the silicon element source in the raw material is unique, namely the silicon element is completely from silicon dioxide, the uniformity of the mixing of the raw materials in the same batch can be inspected by detecting the content of the silicon element in each bag of product and comparing the dispersion of the content of the silicon element in different packaging bags with the theoretical content of the silicon element.
The compound probiotic composition 50kg prepared by the first feeding and mixing according to the proportion of example 1 was subpackaged with 2g per bag. Randomly extracting 3 bags from the bagged composite probiotic composition for element analysis, and detecting the content of silicon element.
In the same way, 3 bags of bagged composite probiotic compositions are randomly extracted from the examples 2 and 3 and the comparative examples 1 and 2 respectively according to the method, and are subjected to element analysis to detect the content of silicon element.
The results are shown in Table 2.
The theoretical silicon content in each bag is M calculated according to the feeding amountSi/MGeneral assembly＝0.467％。
Table 2 silicon content test results of the extracted pouched composite probiotic compositions of examples 1-3 and comparative examples 1-2.
As can be seen from Table 2, the distribution of the silicon content in examples 1-3 is more uniform and closer to the theoretical value of 0.467 wt%. The powder mixing device disclosed by the application is adopted, and when the rotary mixing component and the gas-assisted homogenizing component are adjusted to work together, the uniformity of powder mixing can be obviously improved. Especially, when the process parameters of the powder mixing equipment are controlled in the rotating speed of the rotating mixing component 3 to be 40-90r/min and the pulse period of the pulse airflow is 2-5s, the powder mixing effect is better. Preferably, the rotating mixing assembly 3 rotates at a speed of 60r/min with a pulse period of 3 s.
Example 4
Weighing raw materials including 40021g of inulin, 6000g of galacto-oligosaccharide, 2500g of orange powder, 500g of silicon dioxide, 250g of lactobacillus reuteri, 250g of lactobacillus acidophilus, 250g of bifidobacterium lactis, 62.5g of lactobacillus paracasei and 166.5g of lactobacillus rhamnosus.
The powder is respectively added into each storage tank of the powder mixing equipment, and various raw materials are uniformly mixed according to the process conditions disclosed in the embodiment 1. And (4) feeding the mixed powder into a powder packaging machine, and subpackaging according to 2 g/bag.
After subpackaging, the components in each bag comprise 1.6g of inulin, 0.24g of galacto-oligosaccharide, 0.1g of orange powder, 0.02g of silicon dioxide, 0.01g (10 hundred million CFU) of lactobacillus reuteri, 0.01g (20 hundred million CFU) of lactobacillus acidophilus, 0.01g (30 hundred million CFU) of bifidobacterium lactis, 0.0025g (10 hundred million CFU) of lactobacillus paracasei and 0.0067g (30 hundred million CFU), and the total viable bacteria content of the composite probiotic composition in each bag is 100 hundred million CFU.
Example 5
Weighing raw materials comprising 40260.5g of fructo-oligosaccharide, 6000g of galacto-oligosaccharide, 2500g of orange powder, 500g of silicon dioxide, 250g of lactobacillus reuteri, 187.5g of lactobacillus acidophilus, 125g of bifidobacterium lactis, 93.5g of lactobacillus paracasei and 83.5g of lactobacillus rhamnosus.
The powder is respectively added into each storage tank of the powder mixing equipment, and various raw materials are uniformly mixed according to the process conditions disclosed in the embodiment 1. And (4) feeding the mixed powder into a powder packaging machine, and subpackaging according to 2 g/bag.
After subpackaging, the components in each bag comprise 1.61g of fructo-oligosaccharide, 0.24g of galacto-oligosaccharide, 0.1g of orange powder, 0.02g of silicon dioxide, 0.01g (10 hundred million CFU) of lactobacillus reuteri, 0.0075g (15 hundred million CFU) of lactobacillus acidophilus, 0.005g (15 hundred million CFU) of bifidobacterium lactis, 0.0037g (15 hundred million CFU) of lactobacillus paracasei and 0.0033g (15 hundred million CFU) of lactobacillus rhamnosus. The total viable bacteria content in each bag is 70 hundred million CFU.
Comparative example
Comparative example 3
Weighing raw materials, 39250g of resistant dextrin, 6000g of galacto-oligosaccharide, 50g of orange powder, 2500g of silicon dioxide, 500g of lactobacillus reuteri, 250g of lactobacillus acidophilus, 250g of lactobacillus paracasei and 750g of lactobacillus plantarum.
The powder is respectively added into each storage tank of the powder mixing equipment, and various raw materials are uniformly mixed according to the process conditions disclosed in the embodiment 1. And (4) feeding the mixed powder into a powder packaging machine, and subpackaging according to 2 g/bag.
After subpackaging, the components in each bag comprise 1.57g of fructo-oligosaccharide, 0.24g of galacto-oligosaccharide, 0.1g of orange powder, 0.02g of silicon dioxide, 0.02g (20 hundred million CFU) of lactobacillus reuteri, 0.01g (20 hundred million CFU) of lactobacillus acidophilus, 0.01g (30 hundred million CFU) of lactobacillus paracasei and 0.03g (30 hundred million CFU) of lactobacillus plantarum. The total viable bacteria content in each bag is 100 hundred million CFU.
The viable count of Lactobacillus plantarum in this comparative example was (1000 hundred million CFU/g).
Comparative example 4
Weighing raw materials, 39697.5g of resistant dextrin, 6000g of galacto-oligosaccharide, 2500g of orange powder, 500g of silicon dioxide, 625g of lactobacillus reuteri, 312.5g of lactobacillus acidophilus, 207.5g of bifidobacterium lactis and 157.5g of lactobacillus paracasei.
The powder is respectively added into each storage tank of the powder mixing equipment, and various raw materials are uniformly mixed according to the process conditions disclosed in the embodiment 1. And (4) feeding the mixed powder into a powder packaging machine, and subpackaging according to 2 g/bag.
After subpackaging, the components in each bag comprise 1.588g of resistant dextrin, 0.24g of galacto-oligosaccharide, 0.1g of orange powder, 0.02g of silicon dioxide, 0.025g (25 hundred million CFU) of lactobacillus reuteri, 0.0125kg (25 hundred million CFU) of lactobacillus acidophilus, 0.0083g (25 hundred million CFU) of bifidobacterium lactis and 0.0063g (25 hundred million CFU) of lactobacillus paracasei.
Performance test
Nutrient content detection nutrient content in examples 1, 4 and 5 were respectively detected according to the nutrient content detection items and methods disclosed in "physicochemical analysis of food" (Liuhui, Zhanghua, Tangshirong eds. -Beijing: Chinese textile Press, 2017.12), and the detection results were as follows:
TABLE 3 results of measurement of nutrient components in examples 1, 4 and 5
Test example 1
Oral acute toxicity test
Experimental animals: BALB/c mice with week age of 6-8 weeks and weight of 20-22 g are randomly divided into 5 toxicity experimental groups, wherein the toxicity experimental groups 1-5 are numbered respectively, and each group comprises 20 mice (each half of male and female).
The experimental contents are as follows: referring to the standard GB/T15193.3-2003, oral toxicity tests are performed on 5 groups of mice, maximum tolerated dose method is adopted, the mice in toxicity test groups 1-5 are respectively gavaged with the composite probiotic composition solution prepared from the composite probiotic composition prepared in corresponding examples 1, 4 and 5 and comparative examples 3 and 4, the gavage amount is 0.5ml/20g of the weight of the mice per day, the times are divided into 3 times per day, the survival condition of the mice within 14 days is observed continuously for 14 days. The concentration of the composite probiotic composition in the composite probiotic composition solution is 0.8 g/ml.
The composite probiotic composition solution is prepared by dissolving a composite probiotic composition in sterile distilled water.
Through experiments, mice of 5 toxicity experimental groups do not die, have normal mental state and activity, smooth fur, normal diet, no secretion in respiratory tract, eyes, oral cavity and other places, do not find toxic symptoms, and have weight gain.
Experimental example 2
Anti-allergic performance experiments were performed for examples 1, 4, 5 and comparative examples 3, 4:
allergy is an immune disease, a condition in which immune dysfunction is unbalanced in humans. Allergic patients have significantly elevated serum IgE levels, higher numbers of mast cells and higher numbers of IgE receptors on the cell membrane compared to normal persons. The clinical medicine is proved by the study of cell level: helper T cells and their cytokines of onset play an important opsonizing role for the composition of IgE. Helper cells are divided into two subsets, TH1 and TH2, depending on the species of secreted cytokines. The cytokine produced by TH2 cell has effects mainly in antibody formation and allergy reaction. TH1 and TH2 are mutually regulated through cytokines, under the healthy condition, TH1 and TH2 are mutually balanced and are regulated by helper T cells, and TH2 is over-activated when the regulatory ability of helper T cells is deficient or after contacting with certain foreign proteins or allergens (such as dust mites, pollen or seafood and the like), so that TH2 related cytokines are too high, IgE is promoted to be increased, and the IgE concentration in serum is increased, thereby causing allergy. The extent of allergy can thus be determined by measuring the IgE concentration.
Experimental animals: BALB/c mice with age of 6 weeks and weight of 20-22 g;
the mice are randomly divided into 5 groups of anti-allergic experimental groups and 2 blank groups, and the experimental groups 1-5 and the blank groups 1-2 are numbered respectively. Each group contained 20 mice (male and female halves). The ambient temperature during the experiment was 22 ℃. + -. 2 ℃.
The groups 1-5 were fed daily with the complex probiotic composition solutions obtained in examples 1, 4, 5 and comparative examples 3, 4, respectively, starting on day 1 by gavage. The drug is taken once a day, 0.5mL/20g mouse body weight each time, and is continuously used for 49 days.
The preparation method of the composite probiotic composition solution is the same as that of the composite probiotic composition solution, and the concentration of the composite probiotic composition solution is 0.4 g/ml.
TABLE 4 sources of the Complex probiotic composition fed to the antiallergic experimental group and the blank group
| Grouping | Sources of complex probiotic compositions | Amount of feed |
| | Example 1 | 0.2g/20g mouse weight |
| | Example 4 | 0.2g/20g mouse weight |
| | Example 5 | 0.2g/20g mouse weight |
| | Comparative example 3 | 0.2g/20g mouse weight |
| | Comparative example 4 | 0.2g/20g mouse |
| Blank group | ||
| 1 | — | — |
| | Example 1 | 0.2g/20g mouse weight |
Establishing an allergic animal model:
on day 1 of the start of the experiment, 200. mu.L of an antigen preparation (a suspension comprising 50. mu.g of Ovalbumin (OVA) and 5mg of aluminum hydroxide as an adjuvant) was administered to mice in the antiallergic group by intraperitoneal injection. And re-intraperitoneal injection of 100. mu.L of antigen drug was performed on days 14, 28 and 42 of the experiment.
Collection of serum samples and determination of Ovalbumin (OVA) -specific antibodies:
on days 21, 35, and 49 of the experiment, the tail of the mouse was sampled and the concentration of Ovalbumin (OVA) -specific IgE in plasma (μ g/mL) was measured using a mouse ovalbumin-specific IgE (OVA-sIgE) enzyme-linked immunosorbent assay (ELISA) kit.
Mouse ovalbumin specific IgE (OVA sIgE) ELISA kits were purchased from Eaibo (Wuhan) science and technology, Inc., under product number E0720 m.
Table 5: IgE concentration measurement results
| Group of | | Day 35 | Day 49 |
| | 0.35 | 0.40 | 0.47 |
| | 0.31 | 0.33 | 0.37 |
| | 0.33 | 0.36 | 0.40 |
| | 0.51 | 0.61 | 0.77 |
| | 0.56 | 0.67 | 0.81 |
| | 1.51 | 1.59 | 1.67 |
| | / | / | / |
Note: represents no detection of murine ovalbumin-specific IgE antibodies.
In combination with table 5, it can be seen that the complex probiotic composition with anti-allergic effect prepared by the present application has a significant inhibitory effect on the increase of IgE content in blood of mice in an Ovalbumin (OVA) allergy experiment of the mice.
In the anti-allergy experimental groups 1-3, five specific probiotics are selected: lactobacillus reuteri LE16, Lactobacillus acidophilus NCFM, bifidobacteria Bi-07, Lactobacillus paracasei Lpc-37, Lactobacillus rhamnosus HN001, the five probiotics are combined for use and have more prominent antiallergic effect. The combination of the five probiotics has the effects of regulating the species of intestinal flora, promoting benign symbiosis among the flora and improving the decomposition speed of toxic substances in the intestinal tract, thereby weakening the anaphylactic reaction.
The anti-allergy experimental group 2 selects the composite probiotic composition prepared in example 4, and the ratio of probiotics is adjusted, so that the composite probiotic composition prepared in example 4 has a more obvious anti-allergy effect, while the anti-allergy experimental group 3 selects the composite probiotic composition prepared in example 5, and a better anti-allergy effect is obtained by selecting the ratio of another probiotic.
By comparing the anti-allergic experimental groups 2 and 3, it can be found that inulin is added to the composite probiotic composition of example 4 and fructo-oligosaccharide is added to the composite probiotic composition of example 3 in addition to the difference between the ratio of the microbial inoculum and the total viable count in the composite probiotic compositions of examples 4 and 5 used in the anti-allergic experimental groups 2 and 3, respectively, and the purpose of the adjustment is to prepare a composite probiotic composition with anti-allergic effect suitable for adults (example 4) and a composite probiotic composition with anti-allergic effect suitable for children (example 5).
The more critical difference between the composite probiotic composition of example 4 and the composite probiotic composition of example 5 is the different ratios between the respective flora and the different total amount of live bacteria. Therefore, the adult type composite probiotic composition has higher response speed and better effect on regulating the antiallergic capability of the human body, and the children type composite probiotic composition has more stable regulation on the antiallergic capability and can also reduce the uncomfortable feeling of children when the intestinal flora is changed. The dosage of the bifidobacterium lactis and the lactobacillus rhamnosus in the children type composite probiotic composition is properly reduced, so that the composite probiotic composition is more suitable for the gastrointestinal structure of children.
The composite probiotic composition obtained by the application can be used as a raw material of food, medicine or health-care product, and the prepared food, medicine or health-care product has the effect of improving the anti-allergic capacity of human bodies
The complex probiotic composition prepared in examples 1 to 5 of the present application may be processed into powder, tablet, pill, capsule, etc. and administered.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (10)
1. The composite probiotic composition with the anti-allergic effect is characterized by comprising, by weight, 100-140 parts of galacto-oligosaccharide, 40-60 parts of orange powder, 8-12 parts of silicon dioxide and 14-20 parts of probiotic powder;
the probiotic powder is composed of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus.
2. The complex probiotic composition with antiallergic effect according to claim 1, characterized in that: the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is (4-6): (3-6): (2-6): (1-2): (1.5-3.5).
3. The complex probiotic composition with antiallergic effect according to claim 2, characterized in that: the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is (4-6): (1-1.5): (3-3.5).
4. The complex probiotic composition with antiallergic effect according to claim 3, characterized in that: the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is 5:5:5:1.25: 3.33.
5. Composite probiotic composition with antiallergic effect, according to claims 3 or 4, characterized in that: the composite probiotic composition also comprises 900 parts by weight of inulin 700-.
6. The complex probiotic composition with antiallergic effect according to claim 2, characterized in that: the weight ratio of the lactobacillus reuteri, the lactobacillus acidophilus, the bifidobacterium lactis, the lactobacillus paracasei and the lactobacillus rhamnosus is (4-6): (3-4): (2-3): (1.5-1.8): (1.5-3).
7. The complex probiotic composition with antiallergic effect according to claim 6, characterized in that: the weight ratio of lactobacillus reuteri, lactobacillus acidophilus, bifidobacterium lactis, lactobacillus paracasei and lactobacillus rhamnosus is 5:3.75:2.5:1.87: 1.67.
8. Composite probiotic composition with antiallergic effect, according to claims 6 or 7, characterized in that: the composite probiotic composition also comprises 900 parts by weight of fructo-oligosaccharide 700-.
9. Use of the complex probiotic composition with antiallergic effect according to any one of claims 1 to 8 in the preparation of foods, medicines or health products with antiallergic effect.
10. A formulation comprising a complex probiotic composition according to any one of claims 1 to 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111065534.9A CN113974168B (en) | 2021-09-10 | 2021-09-10 | Composite probiotic composition with anti-allergic effect, application and preparation |
Applications Claiming Priority (1)
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