CN113970535A - Detector and using method thereof - Google Patents
Detector and using method thereof Download PDFInfo
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- CN113970535A CN113970535A CN202111195354.2A CN202111195354A CN113970535A CN 113970535 A CN113970535 A CN 113970535A CN 202111195354 A CN202111195354 A CN 202111195354A CN 113970535 A CN113970535 A CN 113970535A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
Abstract
The invention relates to the technical field of detection equipment, and particularly discloses a detector and a using method thereof. The detector comprises a supporting platform, a fluorescence microscope body, a parallel light-emitting device, a horizontal moving mechanism and an XY moving sliding table, wherein the fluorescence microscope body, the parallel light-emitting device and the horizontal moving mechanism are all arranged on the supporting platform; a vertical driving device is arranged on the fluorescence microscope body, and the driving end of the vertical driving device is provided with an objective lens; meanwhile, the driving end of the horizontal moving mechanism is provided with a plurality of optical filters which are arranged at intervals along the horizontal direction; further, a slide glass is arranged at the driving end of the XY moving sliding table. The detector can automatically replace different optical filters through the horizontal moving mechanism, realizes automatic microscopic examination of a sample to be detected, has high microscopic examination efficiency and accurate detection result, and avoids detection errors caused by manual replacement of the optical filters.
Description
Technical Field
The invention relates to the technical field of detection equipment, in particular to a detector and a using method thereof.
Background
When the analyte is detected based on the cell substrate, clear fluorescent cell images of different color systems are detected by using a fluorescence microscope, and whether the analyte contains the antibody to be detected is judged according to the detected fluorescent cell images of different color systems.
In the prior art, a fluorescence microscope can directly observe a fluorescence cell image in an object to be detected, and filters of different color systems are manually exchanged to judge whether the object to be detected contains an antibody to be detected, but the manual exchange of the filters of different color systems is complicated in operation, operation errors are easy to occur, further, errors occur in an image displayed during observation, and clinical misdiagnosis is easy to cause when a clinician combines a detection result.
Disclosure of Invention
The invention aims to provide a detector and a using method thereof, which are used for solving the problems that in the prior art, when filters of different color systems are manually exchanged, the operation is complicated, the operation error is easy to occur, and further the displayed image has errors during observation.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a testing apparatus, comprising a support platform and:
the fluorescence microscope body is arranged on the supporting platform; the fluorescence microscope body is provided with a vertical driving device, and the driving end of the vertical driving device is provided with an objective lens; the vertical driving device is used for adjusting the height of the objective lens in the vertical direction and the magnification of the objective lens;
the parallel light-emitting device is arranged on the supporting platform and used for providing parallel light for the objective lens;
the driving end of the horizontal moving mechanism is provided with a plurality of optical filters arranged at intervals along the horizontal direction; the horizontal moving mechanism is arranged on the supporting platform and used for adjusting the position of the optical filter in a horizontal plane so that the parallel light can pass through any optical filter and then enter the objective lens;
the drive end of the XY moving sliding table is provided with a glass slide; the XY moving sliding table is arranged on the fluorescence microscope body and used for adjusting the position of the slide glass in the horizontal plane.
Further, the horizontal moving mechanism comprises a first support frame, a second support frame, a motor and a bearing piece, wherein the bearing piece is provided with an optical filter; the first support frame and the second support frame are both arranged on the support platform, the motor is arranged on the first support frame, and the bearing piece is arranged on the second support frame; the driving end of the motor can be meshed with the bearing piece for transmission, so that the bearing piece moves on the second support frame along the horizontal direction.
Further, a rack is arranged on the bearing piece along the horizontal direction, a gear is arranged at the driving end of the motor, and the rack is meshed with the gear.
Furthermore, the fluorescence microscope body is provided with an adapter, the adapter is connected with a camera, and the camera is used for shooting the glass slide.
Further, the filter comprises at least two filters of different color systems.
Furthermore, the light shielding box is sleeved outside the detector.
Further, a fan is further arranged on the supporting platform, an air outlet is formed in the light shielding box, and the fan is located at the air outlet and used for dissipating heat of the detector.
In a second aspect, the present invention provides a method for using a testing apparatus, which is used to test a sample to be tested in the glass slide, and the method for using the testing apparatus is completed by using the testing apparatus; the use method of the detector comprises the following steps:
s1, placing the slide glass on the driving end of the XY moving sliding table;
s2, adjusting the position of the filter by the horizontal moving mechanism so that the parallel light passes through the filter and enters the objective lens;
s3, adjusting the position of the slide by the XY-moving stage, and adjusting the height of the objective lens by the vertical driving device, so that the slide can be aligned with the objective lens, and the detector can detect a clear fluorescent image;
s4, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spots in the fluorescence image;
s5, switching the different filters by the horizontal movement mechanism;
s6, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spot in the fluorescence image when different filters are used;
and S7, judging according to the imaging characteristics of the fluorescent spots in the fluorescent image so as to detect the antibody to be detected in the sample to be detected.
Further, the imaging characteristics of the fluorescent spots include imaging positions, imaging shapes, and fluorescence intensities.
In a third aspect, the present invention provides a method for using a testing apparatus for testing a sample to be tested in the glass slide, wherein the method for using the testing apparatus is completed by using the testing apparatus; the use method of the detector comprises the following steps:
a1, placing the slide glass on the driving end of the XY moving sliding table;
a2, adjusting the position of the filter by the horizontal moving mechanism so that the parallel light passes through the filter and enters the objective lens;
a3, adjusting the position of the slide glass by the XY moving slide, and adjusting the height of the objective lens by the vertical driving device, so that the slide glass can be aligned with the objective lens, and the detector can detect a clear fluorescent image;
a4, observing the fluorescence image detected by the detector to obtain the imaging position, imaging shape and fluorescence intensity of the fluorescence spot in the fluorescence image;
a5, switching the different filters by the horizontal movement mechanism;
a6, repeating the steps A1, A3-A5, and replacing the glass slide added with samples to be detected with different dilution concentration multiple ratios to obtain the imaging position, the imaging shape and the fluorescence intensity of the fluorescence spots in the fluorescence image under the condition of a plurality of samples to be detected with different dilution concentration multiple ratios and under the condition of using different filters;
and A7, judging according to the imaging positions, the imaging shapes and the fluorescence intensities of the fluorescence spots in the multiple fluorescence images, so as to carry out semi-quantitative detection on the antibody to be detected in the sample to be detected.
The invention has the beneficial effects that:
the detector comprises a supporting platform, a fluorescence microscope body, a parallel light-emitting device, a horizontal moving mechanism and an XY moving sliding table, wherein a vertical driving device is arranged on the fluorescence microscope body, an objective lens is arranged at the driving end of the vertical driving device, the XY moving sliding table is further arranged on the fluorescence microscope body, a glass slide is arranged at the driving end of the XY moving sliding table, the height of the objective lens and the magnification factor of the objective lens are adjusted through the vertical driving device, and the position of the glass slide is adjusted through the XY moving sliding table, so that the glass slide can be vertically aligned with the objective lens, and further the detection of a sample to be detected is realized; meanwhile, the driving end of the horizontal moving mechanism is provided with a plurality of optical filters which are arranged at intervals along the horizontal direction, and the horizontal moving mechanism can adjust the positions of the optical filters, so that parallel light emitted by the parallel light-emitting device can pass through any optical filter and then enter the objective lens, and further detection of a sample to be detected is realized under the condition of different optical filters; the detector can automatically replace different optical filters through the horizontal moving mechanism, realizes automatic microscopic examination of a sample to be detected, is high in microscopic examination efficiency, accurate in detection result, and avoids detection errors caused by manual replacement of the optical filters.
The using method of the detector is simple and convenient in using operation, the microscopic examination of the sample to be detected can be automatically realized only by arranging the glass slide at the driving end of the XY moving sliding table, the detection efficiency is high, the detection result has high referential performance, and the detection error caused by manually replacing the optical filter is avoided.
Drawings
Fig. 1 is a schematic structural diagram of a detector provided in an embodiment of the present invention;
FIG. 2 is a side view of a meter provided in accordance with an embodiment of the present invention;
fig. 3 is a partially enlarged view of a portion a in fig. 1.
In the figure:
1. a support platform; 2. a fluorescence microscope body; 21. a vertical drive device; 22. an objective lens; 23. a crossover sub; 3. a parallel light emitting device; 4. a horizontal movement mechanism; 41. a first support frame; 42. a second support frame; 43. a motor; 44. a carrier; 5. moving the sliding table in an XY manner; 6. a glass slide; 7. a camera; 8. a fan.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Where the terms "first position" and "second position" are two different positions, and where a first feature is "over", "above" and "on" a second feature, it is intended that the first feature is directly over and obliquely above the second feature, or simply means that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
The present embodiment provides a test apparatus for testing a specimen to be tested in a slide 6.
As shown in FIGS. 1 to 2, the detector comprises a support platform 1, a fluorescence microscope body 2, a parallel light emitting device 3, a horizontal moving mechanism 4 and an XY moving sliding table 5. Be provided with vertical drive arrangement 21 on fluorescence microscope body 2, vertical drive arrangement 21's drive end is provided with objective 22, and vertical drive arrangement 21 can be used for adjusting objective 22 height and objective 22's magnification in vertical direction, and then when examining the sample that awaits measuring in slide 6, can make fluorescence microscope body 2 receive clear image, wherein, fluorescence microscope body 2 is located on supporting platform 1 to guarantee the holistic stability of fluorescence microscope body 2. Optionally, the fluorescence microscope body 2 is provided with an adapter 23, and the adapter 23 is connected with a camera 7, and the camera 7 is used for shooting the slide glass 6 and storing the shot image. Further optionally, the detecting apparatus further comprises an image receiving component, which is in signal connection with the camera 7 and is used for receiving the image signal captured by the camera 7 and sending the image signal to a computer or other equipment, so as to facilitate a worker to analyze the image captured by the camera 7 for further detecting the sample to be detected in the slide 6.
Further, the parallel light emitting device 3 is arranged on the supporting platform 1 and used for providing parallel light for the objective lens 22, an XY moving sliding table 5 is further arranged on the fluorescence microscope body 2, a glass slide 6 is arranged at the driving end of the XY moving sliding table 5, the height of the objective lens 22 is adjusted through the vertical driving device 21, and the position of the glass slide 6 is adjusted through the XY moving sliding table 5, so that the glass slide 6 can be vertically aligned with the objective lens 22, and further the detection of a sample to be detected is realized; meanwhile, a driving end of the horizontal moving mechanism 4 is provided with a plurality of optical filters arranged at intervals in the horizontal direction, and the horizontal moving mechanism 4 is arranged on the supporting platform 1 and used for adjusting the positions of the optical filters so that parallel light emitted by the parallel light-emitting device 3 can pass through any optical filter and then enter the objective lens 22, thereby realizing detection of a sample to be detected under the condition of different optical filters; the detector can automatically replace different optical filters through the horizontal moving mechanism 4, realizes automatic microscopic examination of a sample to be detected, is high in microscopic examination efficiency, accurate in detection result, and avoids detection errors caused by manual replacement of the optical filters. Optionally, the filter comprises filters of at least two different color systems, in particular, the filter comprises a red filter and a green filter. Of course, the filter further includes other colors, and the embodiment is not particularly limited. Further optionally, the fluorescence microscope body 2 is an inverted fluorescence microscope, and then clear fluorescent cell images of different color systems are detected by the inverted fluorescence microscope, and then the fluorescent cell images of different color systems are judged to detect whether the to-be-detected sample of the glass slide 6 contains the to-be-detected antibody.
As shown in fig. 3, the horizontal moving mechanism 4 includes a first support frame 41, a second support frame 42, a motor 43 and a bearing 44, wherein the bearing 44 is provided with a filter; the first support frame 41 and the second support frame 42 are both arranged on the support platform 1, the motor 43 is arranged on the first support frame 41, the bearing member 44 is arranged on the second support frame 42, the driving end of the motor 43 can be in meshing transmission with the bearing member 44, so that the bearing member 44 moves on the second support frame 42 along the horizontal direction, and further, the automatic replacement of the optical filter is realized through the movement of the bearing member 44 along the horizontal direction, and parallel light emitted by the parallel light-emitting device 3 can pass through the replaced optical filter and then enter the fluorescence microscope body 2. Specifically, a rack is arranged on the bearing piece 44 in the horizontal direction, a gear is arranged at the driving end of the motor 43, and the rack is meshed with the gear, so that the meshed rack and the gear form a gear-rack transmission mechanism, thereby realizing the accurate driving of the bearing piece 44 by the motor 43, ensuring the accurate position of the replaced optical filter, and avoiding the occurrence of detection errors caused by the fact that parallel light emitted by the parallel light-emitting device 3 simultaneously passes through two optical filters.
Further, the outside of detector still is equipped with the light-proof case, and this light-proof case is used for shielding the detector, avoids during external light does not pass the light filter and directly gets into fluorescence microscope body 2, and then influences the use of detector, causes detection error. Wherein, the detector needs to be used under the condition of keeping out of the sun or under the condition of dark external light. Optionally, the supporting platform 1 is further provided with a fan 8, the light shielding box is provided with an air outlet, the fan 8 is located at the air outlet, the detector can be cooled through the fan 8, and the situation that heat in the light shielding box cannot be timely discharged is avoided, so that the detection of the sample to be detected is affected.
The embodiment also provides a using method of the detector, which is used for detecting a sample to be detected in the glass slide 6, and the using method of the detector is completed by using the detector, and comprises the following steps:
s1, placing the slide glass 6 on the driving end of the XY moving sliding table 5;
s2, adjusting the position of the optical filter by the horizontal moving mechanism 4 to enable the parallel light to pass through the optical filter and enter the objective lens 22;
s3, adjusting the position of the slide glass 6 by moving the sliding table 5 in an XY way, and adjusting the height of the objective lens 22 by the vertical driving device 21 so as to align the slide glass 6 with the objective lens 22 and enable the detector to detect a clear fluorescent image;
s4, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spots in the fluorescence image;
s5, switching different filters by the horizontal movement mechanism 4;
s6, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spots in the fluorescence image under the condition of using different optical filters;
and S7, judging according to the imaging characteristics of the fluorescent spots in the fluorescent image so as to detect the antibody to be detected in the sample to be detected.
The using method of the detector provided by the embodiment is simple and convenient in using operation, the microscopic examination of the sample to be detected can be automatically realized only by arranging the glass slide 6 at the driving end of the XY moving sliding table 5, the detection efficiency is higher, the detection result has higher referential performance, and the detection error caused by manual replacement of the optical filter is avoided.
The imaging characteristics of the fluorescent spots comprise imaging positions, imaging shapes and fluorescence intensities, and therefore, under the condition that the sample to be detected is detected by using different optical filters, a worker can accurately judge whether the sample to be detected contains the antibody to be detected or not through the imaging positions, the imaging shapes and the fluorescence intensities. Optionally, the filter is a red filter and a green filter, and the red fluorescence spot and the green fluorescence spot can be respectively displayed on the fluorescence image of the same sample to be detected. Specifically, if the imaging positions of the red fluorescent spot and the green fluorescent spot in the fluorescent image can be overlapped and the imaging shapes are basically the same, it can be determined that the sample to be detected contains the antibody to be detected; if the imaging position of the red fluorescent spot in the fluorescent image cannot be overlapped with the imaging position of the green fluorescent spot in the fluorescent image, or the imaging shape of the red fluorescent spot in the fluorescent image is greatly different from the imaging shape of the green fluorescent spot in the fluorescent image, the sample to be detected can be judged not to contain the antibody to be detected; if the red fluorescent spot exists but the green fluorescent spot does not exist in the fluorescent image, or the green fluorescent spot exists but the red fluorescent spot does not exist in the fluorescent image, it can be determined that the antibody to be detected does not exist in the sample to be detected or the amount of the antibody to be detected is too small.
The present embodiment also provides a second method for using the apparatus for detecting a sample to be detected in a slide 6, which is completed by using the apparatus described above, and comprises the following steps:
a1, placing the slide glass 6 on the driving end of the XY moving sliding table 5;
a2, adjusting the position of the optical filter by the horizontal moving mechanism 4, so that the parallel light enters the objective lens 22 after passing through the optical filter;
a3, adjusting the position of the slide glass 6 by moving the sliding table 5 in an XY way, and adjusting the height of the objective lens 22 by the vertical driving device 21, so that the slide glass 6 can be aligned with the objective lens 22, and the detector can detect a clear fluorescent image;
a4, observing the fluorescence image detected by the detector to obtain the imaging position, the imaging shape and the fluorescence intensity of the fluorescence spot in the fluorescence image;
a5, switching different filters by the horizontal movement mechanism 4;
a6, repeating the steps A1 and A3-A5, and replacing the glass slide 6 added with the samples to be detected with different dilution concentration multiple ratios to obtain the imaging position, the imaging shape and the fluorescence intensity of the fluorescence spots in the fluorescence image under the condition of a plurality of samples to be detected with different dilution concentration multiple ratios and under the condition of using different optical filters;
and A7, judging according to the imaging positions, the imaging shapes and the fluorescence intensities of the fluorescence spots in the multiple fluorescence images, so as to carry out semi-quantitative detection on the antibody to be detected in the sample to be detected.
Specifically, the corresponding to-be-detected antibodies with different concentrations in the to-be-detected samples with different dilution concentration ratios can be used for judging the concentration of the to-be-detected antibodies in the to-be-detected samples through the fluorescence intensity of the fluorescence spots, so that the concentration range of the to-be-detected antibodies can be obtained by the staff according to the detected fluorescence intensity range, and the concentration range of the to-be-detected antibodies in the to-be-detected samples with any dilution concentration ratios can be conveniently and quickly detected by the staff. According to the judgment of the imaging position and the imaging shape, the influence of other substances except the antibody to be detected in the sample to be detected on the semi-quantitative detection of the antibody to be detected can be prevented.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A detector, characterized in that it comprises a support platform (1) and:
the fluorescence microscope body (2), the fluorescence microscope body (2) is arranged on the supporting platform (1); a vertical driving device (21) is arranged on the fluorescence microscope body (2), and an objective lens (22) is arranged at the driving end of the vertical driving device (21); the vertical driving device (21) is used for adjusting the height of the objective lens (22) in the vertical direction and the magnification of the objective lens (22);
the parallel light-emitting device (3) is arranged on the supporting platform (1) and used for providing parallel light for the objective lens (22);
the driving end of the horizontal moving mechanism (4) is provided with a plurality of optical filters which are arranged at intervals along the horizontal direction; the horizontal moving mechanism (4) is arranged on the supporting platform (1) and is used for adjusting the position of the optical filter in a horizontal plane so that the parallel light can enter the objective lens (22) after passing through any optical filter;
the XY moving sliding table (5), the driving end of the XY moving sliding table (5) is provided with a glass carrying sheet (6); the XY moving sliding table (5) is arranged on the fluorescence microscope body (2) and used for adjusting the position of the glass slide (6) in the horizontal plane.
2. The detecting instrument according to claim 1, characterized in that the horizontal moving mechanism (4) comprises a first support (41), a second support (42), a motor (43) and a bearing (44), wherein the bearing (44) is provided with a filter; the first support frame (41) and the second support frame (42) are arranged on the support platform (1), the motor (43) is arranged on the first support frame (41), and the bearing piece (44) is arranged on the second support frame (42); the driving end of the motor (43) can be in meshing transmission with the bearing piece (44) so that the bearing piece (44) can move on the second supporting frame (42) along the horizontal direction.
3. The meter according to claim 2, wherein the carrier (44) is provided with a rack along the horizontal direction, and the drive end of the motor (43) is provided with a gear, the rack being in mesh with the gear.
4. The apparatus according to claim 1, characterized in that the fluorescence microscope body (2) is provided with an adapter (23), the adapter (23) being connected to a camera (7), the camera (7) being used for photographing the slide (6).
5. The meter of claim 1, wherein the filter comprises filters of at least two different colour systems.
6. The meter of claim 1, wherein the meter is further externally fitted with a light shielding box.
7. The detector according to claim 6, wherein a fan (8) is further disposed on the supporting platform (1), an air outlet is disposed on the light shielding box, and the fan (8) is located at the air outlet and used for dissipating heat of the detector.
8. Use of a device for detecting a sample to be tested in a slide (6), characterized in that the use of the device is carried out using a device according to any one of claims 1 to 7; the use method of the detector comprises the following steps:
s1, placing the slide glass (6) on the driving end of the XY moving sliding table (5);
s2, adjusting the position of the optical filter through the horizontal moving mechanism (4) so that the parallel light enters the objective lens (22) after passing through the optical filter;
s3, adjusting the position of the slide glass (6) through the XY moving sliding table (5), and adjusting the height of the objective lens (22) through the vertical driving device (21) so that the slide glass (6) can be aligned with the objective lens (22) and a clear fluorescent image can be detected by the detector;
s4, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spots in the fluorescence image;
s5, switching different optical filters through the horizontal moving mechanism (4);
s6, observing the fluorescence image detected by the detector to obtain the imaging characteristics of the fluorescence spots in the fluorescence image under the condition of using different optical filters;
and S7, judging according to the imaging characteristics of the fluorescent spots in the fluorescent image so as to detect the antibody to be detected in the sample to be detected.
9. The method of claim 8, wherein the imaging characteristics of the fluorescent spot include imaging location, imaging shape and fluorescence intensity.
10. Use of a device for detecting a sample to be tested in a slide (6), characterized in that the use of the device is carried out using a device according to any one of claims 1 to 7; the use method of the detector comprises the following steps:
a1, placing the slide glass (6) on the driving end of the XY moving sliding table (5);
a2, adjusting the position of the optical filter by the horizontal moving mechanism (4) so that the parallel light enters the objective lens (22) after passing through the optical filter;
a3, adjusting the position of the slide glass (6) through the XY moving sliding table (5), and adjusting the height of the objective lens (22) through the vertical driving device (21) so as to align the slide glass (6) with the objective lens (22) and enable the detector to detect a clear fluorescence image;
a4, observing the fluorescence image detected by the detector to obtain the imaging position, the imaging shape and the fluorescence intensity of a fluorescence spot in the fluorescence image;
a5, switching different filters by the horizontal movement mechanism (4);
a6, repeating the steps A1, A3-A5, and replacing the glass slide (6) added with samples to be detected with different dilution concentration multiple ratios to obtain the imaging position, the imaging shape and the fluorescence intensity of the fluorescence spots in the fluorescence image under the condition of a plurality of samples to be detected with different dilution concentration multiple ratios and under the condition of using different optical filters;
and A7, judging according to the imaging positions, the imaging shapes and the fluorescence intensities of the fluorescence spots in the multiple fluorescence images, so as to carry out semi-quantitative detection on the antibody to be detected in the sample to be detected.
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