CN113964014B - Ion fragmentation device and method based on linear ion trap mass spectrometer - Google Patents
Ion fragmentation device and method based on linear ion trap mass spectrometer Download PDFInfo
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Classifications
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/10—Ion sources; Ion guns
Abstract
The invention provides an ion fragmentation device and method based on a linear ion trap mass spectrometer, comprising a columnar electrode, an end cover electrode, a radio frequency power supply RF, a first direct current power supply DC1, a second direct current power supply DC2 and a third direct current power supply DC3; the end cover electrode comprises a front end cover electrode and a rear end cover electrode, and the front end cover electrode and the rear end cover electrode are arranged on two sides of the columnar electrode; the radio frequency power supply RF is connected with a plurality of pairs of columnar electrodes; the first direct current power supply DC1 is connected with the front end cover electrode; the second direct current power supply DC2 is connected with the rear end cover electrode; the third direct current power supply DC3 is connected with the columnar electrode. The invention can realize ion fragmentation by adjusting the amplitude of the direct current voltage, is simple and easy to operate, simplifies a circuit system and reduces the cost.
Description
Technical Field
The invention relates to the technical field of mass spectrometry, in particular to an ion fragmentation device and method based on a linear ion trap mass spectrometer.
Background
Mass spectrometry is a method of qualitatively and quantitatively analyzing a sample by separating and measuring ions according to the mass-to-charge ratio (m/z) of the ions. Instruments for performing mass spectrometry, called mass spectrometers, have been widely used in the fields of biological medicine, environmental analysis, manned aerospace, criminal science and technology, life sciences, and the like. Tandem Mass Spectrometry (MS) n ) Is an important means of analysis of mixtures and molecular structure identification and has long been used in large mass spectrometers. In particular in the interdisciplinary disciplines of chemistry and life sciences, systematic analysis of proteomes using tandem mass spectrometry, and the discovery and intensive research of post-translational modifications of specific proteinsAll are of importance.
Tandem mass spectrometry is generally classified into spatial tandem and temporal tandem according to the connection mode. Spatial tandem refers to the connection of more than two mass analyzers together, such as triple quadrupole tandem (QQQ), quadrupole-time of flight tandem (Q-TOF), and the like. Only one mass analyzer is needed for time series connection, and cascade analysis is realized by arranging different stages, such as an ion trap, an ion cyclotron resonance mass spectrometer and the like. From the cost perspective, the ion trap mass spectrometer can realize multistage tandem mass spectrometry by only using a single mass analyzer, and has unique advantages. Among them, linear ion traps have better ion trapping efficiency and storage capacity than three-dimensional ion traps, and have received much attention.
In tandem mass spectrometry, molecular ions are subjected to multi-stage cleavage to obtain abundant compound fragment information, which is used for deducing the structure of a compound and identifying a target compound. Identification of overlapping chromatographic peaks can be achieved in chromatography-mass spectrometry and quantification of target compounds in the presence of high background or interferents. The ion trap completed tandem mass spectrum mainly comprises the following steps: 1. selecting proper q value according to the ion trap stability diagram, selecting target ions to store in the ion trap, and expelling other ions out of the ion trap; 2. performing collision-induced dissociation, and increasing ion kinetic energy to enable the ion to collide and disintegrate with buffer gas in a trap; 3. mass scanning is performed to obtain a mass spectrum of the product ions, which is accomplished by scanning the radio frequency voltage amplitude. The above steps are accomplished by computer control, and multi-stage (MSn) experiments can be performed as necessary.
Conventional Collision Induced Dissociation (CID) is a process of applying a sine wave (AC) with the same resonance frequency to a parent ion with a specific mass-to-charge ratio in an ion trap to collide with gas molecules in the trap, thereby causing ion dissociation, and is one of effective methods for deducing the structure of the parent ion by fragment ions, and is widely used in tandem mass spectrometry (MSn). In a linear ion trap, a sine wave for collision induced dissociation is typically applied to one of the pairs of electrodes.
Patent document publication No. CN101061564a discloses an ion trap for a mass spectrometer comprising: an RF trapping voltage supply for applying an RF trapping voltage to at least one of the plurality of electrodes of the ion trap to trap at least a portion of ions in the ion trap; a resonance excitation voltage source for applying a resonance excitation voltage pulse to the electrode to cause at least a portion of the selected ion set to be collided and broken into ion fragments; and a computer for controlling the RF trapping voltage supply to reduce the RF trapping voltage to a second amplitude after a predetermined delay period for maintaining low mass ion fragments in the ion trap for subsequent analysis, the delay period following termination of the resonance excitation voltage pulse. Patent document with publication number CN107731655a discloses a method for ion fragmentation based on quadrupole-linear ion trap tandem mass spectrometer, which is to select target ions from ionized ions by using quadrupole rods and make the target ions enter an ion trap, cool the ions after accumulating certain concentration in the ion trap, raise the radio frequency voltage of the quadrupole rods to 1/3 of the same voltage as the target ions, and simultaneously apply radio frequency electric field to the ion trap to realize fragmentation of the target ions stored in the ion trap. Patent document publication No. CN107799381B discloses a mass spectrometer for realizing ion dissociation between bilinear ion traps, comprising a first linear ion trap and a second linear ion trap, which are serially connected and arranged at intervals to construct an ion channel extending along an axial direction, wherein the ion channel comprises trapping regions at two sides and a collision region at the middle part; the first electrode is arranged at one end of the first linear ion trap; the second electrode is arranged between the first linear ion trap and the second linear ion trap; and the third electrode is arranged at the other end of the second linear ion trap. However, the above patent documents have a drawback in that the implementation device is complicated.
Disclosure of Invention
In view of the defects in the prior art, the invention aims to provide an ion fragmentation device and method based on a linear ion trap mass spectrometer.
The invention provides an ion fragmentation device based on a linear ion trap mass spectrometer, which comprises a columnar electrode, an end cover electrode, a radio frequency power supply RF, a first direct current power supply DC1, a second direct current power supply DC2 and a third direct current power supply DC3, wherein the columnar electrode is connected with the end cover electrode;
the end cover electrode comprises a front end cover electrode and a rear end cover electrode, and the front end cover electrode and the rear end cover electrode are arranged on two sides of the columnar electrode;
the radio frequency power supply RF is connected with a plurality of pairs of columnar electrodes; the first direct current power supply DC1 is connected with the front end cover electrode; the second direct current power supply DC2 is connected with the rear end cover electrode; the third direct current power supply DC3 is connected with the columnar electrode.
Preferably, the columnar electrodes are arranged in at least two pairs, and the columnar electrodes are parallel to each other.
Preferably, the polarities of the output DC signals of the first DC power supply DC1, the second DC power supply DC2, and the third DC power supply DC3 are the same.
Preferably, the polarity of the output DC signals of the first DC power supply DC1, the second DC power supply DC2, and the third DC power supply DC3 is the same as the polarity of the target ion.
Preferably, the output voltage amplitude of the first direct current power supply DC1 is-100V.
Preferably, the output voltage amplitude of the second direct current power supply DC2 is +/-40V to +/-100V.
Preferably, the output voltage amplitude of the third direct current power supply DC3 is-100V.
Preferably, the device is internally filled with a quantity of background gas molecules.
The invention also provides an ion fragmentation method based on the ion fragmentation device based on the linear ion trap mass spectrometer, which comprises the following steps:
step 1: pulling down the voltage amplitude of a first direct current power supply DC1 on the electrode of the front end cover to enable target ions to enter the ion fragmentation device;
step 2: after sample injection is completed, the voltage on the electrode of the front end cover is raised to be the same as the voltage amplitude of the second direct current power supply DC 2;
step 3: the amplitude of the radio frequency voltage on the columnar electrode is regulated to ensure that ions are bound in the linear ion trap to stably move;
step 4: and adjusting the voltage amplitude value applied by the third direct current power supply DC3 on the columnar electrode, and increasing the depth of the potential well in the linear ion trap, so that the ion energy is increased, the collision between ions and buffer gas is aggravated, and the fragmentation of target ions is realized.
Preferably, in the step 2, the voltage on the front end cover electrode is kept unchanged all the time.
Compared with the prior art, the invention has the following beneficial effects:
1. the ion fragmentation can be realized by adjusting the amplitude of the direct-current voltage, the ion fragmentation method is simple and easy to implement, the circuit system is simplified, and the cost is reduced;
2. the method does not need to apply auxiliary resonance excitation signals, has no low-mass cut-off effect and does not lose low-mass ions;
3. the ion fragmentation efficiency of the invention is higher, and the chemical structure information of the substance can be provided more abundantly.
Drawings
Other features, objects and advantages of the present invention will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a three-dimensional view of an ion fragmentation device for a linear ion trap mass spectrometer in accordance with the present invention;
FIG. 2 is a cross-sectional view of an ion fragmentation device for a linear ion trap mass spectrometer in accordance with the present invention;
FIG. 3 is a graph showing the abundance of reserpine parent ions (m/z 609) in a device as a function of the absolute value of the difference between DC3 and DC2 (DC voltage) for the ion fragmentation method used in example one;
FIG. 4 is a mass spectrum peak diagram of parent ion before reserpine (m/z 609) ion fragmentation in example one;
FIG. 5 is a graph of ion fragmentation peaks generated using the conventional CID method in accordance with example one;
FIG. 6 is a graph of ion fragmentation peaks generated by the ion fragmentation device and method according to the present invention in the first embodiment;
FIG. 7 is a diagram showing the number of ion fragments generated by different substances in the conventional CID mode and under the conditions of the present invention in the first embodiment.
The figure shows:
front end cap electrode 201 of columnar electrode 1
End cap electrode 2 rear end cap electrode 202
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
As shown in fig. 1 and fig. 2, the ion fragmentation device based on a linear ion trap mass spectrometer provided by the invention comprises a columnar electrode 1, an end cover electrode 2, a radio frequency power supply RF, a first direct current power supply DC1, a second direct current power supply DC2 and a third direct current power supply DC3, wherein the end cover electrode 2 comprises a front end cover electrode 201 and a rear end cover electrode 202, the front end cover electrode 201 and the rear end cover electrode 202 are arranged on two sides of the columnar electrode 1, and the radio frequency power supply RF is connected with a plurality of pairs of columnar electrodes 1; the first DC power supply DC1 is connected with the front end cover electrode 201, the second DC power supply DC2 is connected with the rear end cover electrode 202, and the third DC power supply DC3 is connected with the columnar electrode 1. The columnar electrodes 1 are arranged in at least two pairs, and the columnar electrodes 1 are parallel to each other. The interior of the device is filled with a certain amount of background gas molecules.
The polarities of the output direct current signals of the first direct current power supply DC1, the second direct current power supply DC2 and the third direct current power supply DC3 are the same, and the polarities of the output direct current signals of the first direct current power supply DC1, the second direct current power supply DC2 and the third direct current power supply DC3 are the same as the polarities of target ions. The output voltage amplitude of the first direct current power supply DC1 is-100V, the output voltage amplitude of the second direct current power supply DC2 is +/-40V- +/-100V, and the output voltage amplitude of the third direct current power supply DC3 is-100V.
The absolute value of the voltage difference between the voltage of the third direct current power supply DC3, the voltage of the first direct current power supply DC1 and the voltage difference of the second direct current power supply DC2 is larger than or equal to 1/8 of the product of the q value corresponding to the trapped ions when the voltage of the radio frequency power supply is applied in the device and the voltage amplitude Vrf of the radio frequency power supply, wherein the q value has the following calculation formula:
wherein the q value represents a dimensionless parameter, V rf Represents the amplitude of the output voltage of the radio frequency power supply, ω represents the frequency of the radio frequency power supply, r 0 Representing the linear ion trap field radius and m/e represents the mass to charge ratio of the ions.
Working principle:
the radio frequency power supply RF is applied to the columnar electrode 1 for capturing bound ions, the first direct current voltage source DC1 and the second direct current voltage source DC2 are respectively applied to the front end cover electrode 201 and the rear end cover electrode 202, so that the introduction and the trapping of ions are realized, and the third direct current source DC3 is applied to the columnar electrode 1 to form an axial (z direction) potential well. By adjusting the absolute values of the voltage difference between the third DC power supply DC3 and the voltage of the first DC power supply DC1 and the voltage difference between the second DC power supply DC2, the q value corresponding to the voltage of the radio frequency power supply RF applied by the trapped ions in the device is larger than or equal to the voltage amplitude V of the radio frequency power supply rf 1/8 of the product, ion fragmentation is realized.
The invention also provides an ion fragmentation method based on the ion fragmentation device based on the linear ion trap mass spectrometer, which comprises the following steps:
step 1: pulling down the voltage amplitude of the first direct current power supply DC1 on the front end cover electrode 201 to enable target ions to enter the ion fragmentation device;
step 2: after the sample injection is completed, the voltage on the front end cover electrode 201 is pulled up to be the same as the voltage amplitude of the second direct current power supply DC2, and in the step 2, the voltage on the front end cover electrode 201 is kept unchanged all the time;
step 3: the amplitude of the radio frequency voltage on the columnar electrode 1 is regulated to ensure that ions are bound in the linear ion trap to stably move;
step 4: the voltage amplitude applied by the third direct current power supply DC3 on the columnar electrode 1 is adjusted, the depth of the potential well in the linear ion trap is increased, so that the ion energy is increased, the collision between ions and buffer gas is aggravated, and the fragmentation of target ions is realized.
In the preferred embodiment, in step 2, after the sample injection is completed, the voltage on the front end cover electrode 201 is pulled up to a higher value with the same magnitude as the voltage of the second DC power supply DC2, so as to prevent ions from entering, and in this process, the magnitude of the voltage of the second DC power supply DC2 on the rear end cover electrode 202 is always a higher value, so as to prevent ions from escaping.
Example 1:
an ion fragmentation device based on a linear ion trap mass spectrometer comprises two pairs of hyperboloid columnar electrodes and a pair of end cover electrodes, wherein the pair of end cover electrodes are respectively a front end cover electrode and a rear end cover electrode. The first direct current power is applied to the front end cover electrode, and the second direct current power is applied to the rear end cover electrode. Fig. 2 is a radial cross-sectional view of an ion fragmentation device of a linear ion trap mass spectrometer, where a radio frequency power source outputs two paths of sinusoidal signals with the same amplitude and opposite phases, which are respectively applied to two pairs of columnar electrodes, and a third dc power source is applied to the columnar electrodes. In the present embodiment, the columnar electrode is a hyperboloid electrode, and the field radius (r 0 ) At 5mm, the target ion was reserpine (m/z 609), generated by an electrospray ionization source, positive in polarity, and transmitted through an ion optical lens to an ion fragmentation device.
The sample injection process of the ion fragmentation device is as follows: the voltage output amplitude of the second direct current power supply applied to the rear end cover electrode is fixed to be 40V direct current voltage, and the output voltage of the first direct current voltage source applied to the front end cover electrode is reduced from 40V to 2.6V, so that the target ion trap enters the ion fragmentation device. After sample injection is completed, the voltage of the first direct current power supply is increased to 40V, and the voltage value of the first direct current power supply is the same as that of the second direct current power supply. And applying radio frequency voltage to the hyperboloid columnar electrode to bind ions. And adjusting the voltage amplitude of the third direct current power supply, and changing the depth of a potential well in the device to cause ion fragmentation.
In this embodiment, the absolute value DC voltage of the difference between the voltages of the third DC power supply and the second DC power supply ranges from 20V to 48V, one set of data is obtained every 4V, 8 sets of data are used, and the ion abundance change of the reserpine parent ion m/z 609 is shown in fig. 3. Amplitude V of radio frequency power supply rf 525V, frequency of 0.96MHz, according to field radius r 0 =5 mm, and the q value is calculated to be 0.365, and the calculated value is obtained by substituting the potential well calculation formula: d (D) well ＝1/8*V rf * q=23.96V, which is the DC voltage threshold for ion fragmentation. As shown in fig. 3, when the DC voltage is 24V, the m/z 609 ion abundance is highest, and at this time, the ion collides with the buffer gas in the trap under the action of the third direct current voltage, so that the extra kinetic energy of the ion is eliminated and the ion cannot be broken, and a high signal intensity is obtained. With the gradual increase of the DC voltage value (> 24V), the deeper the potential well depth inside the device, the stronger the kinetic energy of the incoming ions, and the more serious the collisions between the ions and the buffer gas, so that the ions are fragmented.
In this example, the peak spectra of the ions generated by reserpine parent ion (m/z 609) in the conventional CID mode and the proposed method of the present invention are compared, as shown in FIGS. 4 to 6. The more kinds of sub-ion peaks generated under the condition of the invention are compared with the CID, which means that the chemical information and the structural information of the substances can be provided more abundantly. Meanwhile, the invention has no low-quality effect cut-off, and can observe low-quality fragment ions (m/z 178) of reserpine on a spectrogram, and the low-quality cut-off (LMCO) effect exists under CID mode because of the selected q value, so that the low-quality ions cannot be captured obviously, and the ion fragmentation efficiency is reduced.
In order to verify the universality of the invention to other substances, ion fragmentation conditions of 25OH vitamins D2 and D3 under the conditions of the conventional CID mode and the fragmentation device and method disclosed by the invention are also analyzed in the embodiment, and histograms are listed for comparison in combination with the reserpine analysis results. As shown in FIG. 7, the number of ion fragmentation peaks generated by different substances under the condition of the invention is more than that generated by the traditional CID mode, and the ion fragmentation efficiency of the invention is higher than that of the traditional method, thus the invention has the advantages of creativity and advancement.
The invention can realize ion fragmentation by adjusting the amplitude of the direct current voltage, is simple and easy to operate, simplifies a circuit system and reduces the cost.
In the description of the present application, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, merely to facilitate description of the present application and simplify the description, and do not indicate or imply that the devices or elements being referred to must have a specific orientation, be configured and operated in a specific orientation, and are not to be construed as limiting the present application.
The foregoing describes specific embodiments of the present invention. It is to be understood that the invention is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the invention. The embodiments of the present application and features in the embodiments may be combined with each other arbitrarily without conflict.
Claims (8)
1. The ion fragmentation method is characterized in that an ion fragmentation device based on a linear ion trap mass spectrometer is adopted, and comprises a columnar electrode (1), an end cover electrode (2), a radio frequency power supply RF, a first direct current power supply DC1, a second direct current power supply DC2 and a third direct current power supply DC3;
the end cover electrode (2) comprises a front end cover electrode (201) and a rear end cover electrode (202), and the front end cover electrode (201) and the rear end cover electrode (202) are arranged on two sides of the columnar electrode (1);
the radio frequency power supply RF is connected with a plurality of pairs of columnar electrodes (1); the first direct current power supply DC1 is connected with the front end cover electrode (201); the second direct current power supply DC2 is connected with the rear end cover electrode (202); the third direct current power supply DC3 is connected with the columnar electrode (1);
the columnar electrodes (1) are at least arranged in two pairs, and the columnar electrodes (1) are parallel to each other;
the method comprises the following steps:
step 1: pulling down the voltage amplitude of a first direct current power supply DC1 on a front end cover electrode (201) to enable target ions to enter the ion fragmentation device;
step 2: after sample injection is completed, the voltage on the front end cover electrode (201) is pulled up to be the same as the voltage amplitude of the second direct current power supply DC 2;
step 3: the amplitude of the radio frequency voltage on the columnar electrode (1) is regulated to ensure that ions are bound in the linear ion trap to stably move;
step 4: and adjusting the voltage amplitude applied by a third direct current power supply DC3 on the columnar electrode (1) to increase the depth of a potential well in the linear ion trap, so that the ion energy is increased, the collision between ions and buffer gas is aggravated, and the fragmentation of target ions is realized.
2. The ion fragmentation method of claim 1, in which in step 2, the voltage on the front end cap electrode (201) is kept constant at all times.
3. The ion fragmentation method of claim 1, wherein the polarity of the output DC signals of the first DC power source DC1, the second DC power source DC2, and the third DC power source DC3 are the same.
4. The ion fragmentation method of claim 1, wherein the polarity of the output DC signals of the first DC power supply DC1, the second DC power supply DC2, and the third DC power supply DC3 is the same as the polarity of the target ion.
5. The ion fragmentation method of claim 1, wherein the output voltage amplitude of the first direct current power supply DC1 is-100 v.
6. The ion fragmentation method of claim 1, wherein the output voltage amplitude of the second DC power source DC2 is ±40v to ±100deg.v.
7. The ion fragmentation method of claim 1, wherein the output voltage amplitude of the third DC power supply DC3 is-100 v.
8. The ion fragmentation method of claim 1, wherein the device is internally filled with a quantity of background gas molecules.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6545268B1 (en) * | 2000-04-10 | 2003-04-08 | Perseptive Biosystems | Preparation of ion pulse for time-of-flight and for tandem time-of-flight mass analysis |
| US7232993B1 (en) * | 2005-12-23 | 2007-06-19 | Varian, Inc. | Ion fragmentation parameter selection systems and methods |
| CN102169791A (en) * | 2010-02-05 | 2011-08-31 | 岛津分析技术研发（上海）有限公司 | Tandem mass spectrometry apparatus and mass spectrometry method |
| CN105655224A (en) * | 2016-03-09 | 2016-06-08 | 清华大学 | Small two-stage vacuum rectangular ion trap mass spectrometer and detection method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017126006A1 (en) * | 2016-01-18 | 2017-07-27 | 株式会社島津製作所 | Ion trap mass spectrometry device and mass spectrometry method using said device |
| US11562895B2 (en) * | 2018-09-07 | 2023-01-24 | Dh Technologies Development Pte. Ltd. | RF ion trap ion loading method |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6545268B1 (en) * | 2000-04-10 | 2003-04-08 | Perseptive Biosystems | Preparation of ion pulse for time-of-flight and for tandem time-of-flight mass analysis |
| US7232993B1 (en) * | 2005-12-23 | 2007-06-19 | Varian, Inc. | Ion fragmentation parameter selection systems and methods |
| CN102169791A (en) * | 2010-02-05 | 2011-08-31 | 岛津分析技术研发（上海）有限公司 | Tandem mass spectrometry apparatus and mass spectrometry method |
| CN105655224A (en) * | 2016-03-09 | 2016-06-08 | 清华大学 | Small two-stage vacuum rectangular ion trap mass spectrometer and detection method thereof |
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