CN113952314B - Tea polyphenol-gingerol combined particles and preparation method and application thereof - Google Patents
Tea polyphenol-gingerol combined particles and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides tea polyphenol-gingerol combined particles, a preparation method and application thereof, wherein the tea polyphenol-gingerol combined particles comprise the following components in parts by weight: 150-300 parts of tea polyphenol particles and 50-100 parts of ginger extract emulsion, wherein the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid. According to the invention, the hydroxypropyl methylcellulose solution is used for wrapping tea polyphenol to form a first coating, the ginger extract can cover the pungency after being embedded by the emulsifier, meanwhile, the tea polyphenol particles are embedded in the first coating, and finally, the targeting wall material top spray coating is used for realizing multi-layer composite coating, so that the pungency of gingerol and the astringency of tea polyphenol can be covered at the same time, the addition amount of the functional components can be increased, and the complex process and the cost waste caused by separately embedding the gingerol and the tea polyphenol can be avoided.
Description
Technical Field
The invention belongs to the technical field of weight-losing products, and particularly relates to tea polyphenol-gingerol combined particles, a preparation method and application thereof.
Background
According to the report of nutrition and chronic disease status of Chinese residents (2020) and a survey result published in PLoS One journal in 2019, the overweight obesity of adult residents in China is over 50% currently, and the overall prevalence rate of abdominal obesity in adult population is 29.1%. The abdominal obesity rate in China is higher than the common obesity rate, and the ratio of the abdominal obesity rate to the common obesity rate is more continuously increasing. More researches show that the abdominal obesity is more prone to various chronic diseases such as diabetes, coronary heart disease, cardiovascular diseases, cancer and the like than the common obesity. Wherein, the risk of the abdominal adiposity is 6.6 times higher than that of the ordinary adiposity; people with waistline more than or equal to 85 cm have coronary heart disease risk obviously higher than BMI > 25; every 1 inch (2.52 cm) of waist increases, the risk of cancer increases by a factor of 8. Thus, in the pandemic trend of obesity, attention to abdominal obesity appears to be more important.
Functional foods for reducing fat in the abdomen of japan have been popular for many years, mainly comprising ellagic acid and pueraria flower isoflavone, and are favored by central obesity. Currently, few products aiming at abdominal fat reduction are on the market in China, and the products mainly focus on whole body weight reduction by taking the means of poor heat quantity, blocking the absorption of glycolipid and suppressing appetite.
Green tea is an indispensable part of the daily life of people in China, is commonly used in traditional products such as teabags, green tea drinks, green tea cakes and the like, and has good effects of improving immunity and resisting aging. Ginger is a necessary living diet and is widely applied to diets (stir-frying, soup cooking, dish mixing and the like), cold dispelling products, uterus warming products, anti-vomiting products and the like.
Gingerol and tea polyphenol are currently applied to the field of lipid-lowering and weight-losing beverages (see CN101564067A green tea ginger lipid-lowering and weight-losing beverage, CN104543134A ginger green tea beverage and a preparation method thereof), but are not reported in the field of abdominal obesity and lipid-lowering. The gingerol and tea polyphenol applied to the field of lipid-lowering and weight-losing beverages are mainly prepared by adopting simple raw material combination leaching of ginger, green tea and the like, and the obtained product has very low gingerol and tea polyphenol content; meanwhile, the problems of low water solubility of gingerol and poor stability and easiness in oxidization of tea polyphenol in the product are not effectively solved.
Disclosure of Invention
The invention aims to provide tea polyphenol-gingerol combined particles, which overcome the technical problems in the prior art.
The invention also aims to provide a preparation method of the tea polyphenol-gingerol combined particles, which can cover the pungency of gingerol and the astringency of tea polyphenol.
The invention also aims to provide an application of the tea polyphenol-gingerol combined particles in preparing medicines for treating abdominal obesity, and the effects of reducing the whole body weight and reducing the abdominal fat are good.
Therefore, the technical scheme provided by the invention is as follows:
the tea polyphenol-gingerol combined particles comprise the following components in parts by weight: 150-300 parts of tea polyphenol particles and 50-100 parts of ginger extract emulsion, wherein the particle size of the polyphenol particles is 100-150 meshes, and the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid.
The coating liquid is hydroxypropyl methylcellulose-polyethylene glycol mixed solution.
The tea polyphenol particles comprise the following components in parts by weight: 20-200 parts of tea polyphenol and 25-50 parts of hydroxypropyl methyl cellulose solution, wherein the mass concentration of the hydroxypropyl methyl cellulose solution is 2-8%, and the purity of the tea polyphenol is 40-98%.
The ginger extract emulsion comprises the following components in parts by weight: 50-150 parts of ginger extract, 10-25 parts of acacia, 10-25 parts of maltodextrin, 0.5-1.5 parts of sodium stearoyl lactylate and 250-450 parts of water.
The mass ratio of the hydroxypropyl methylcellulose to the polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol mixed solution is 4:1-10:5.
the ginger extract contains 5% -60% of gingerol.
A preparation method of tea polyphenol-gingerol combined particles comprises the following steps:
step 1) preparing tea polyphenol particles;
step 2) preparing ginger extract emulsion;
step 3) taking the tea polyphenol particles as a core material, taking ginger extract emulsion as coating liquid, and performing bottom spraying coating to obtain the tea polyphenol-gingerol particles;
and 4) taking the tea polyphenol-gingerol particles as core materials, taking a hydroxypropyl methyl cellulose-polyethylene glycol mixed solution as coating liquid, and performing top spraying coating to obtain the tea polyphenol-gingerol combined particles.
The preparation process of the tea polyphenol particles comprises the following steps: tea polyphenol is used as a core material, hydroxypropyl methylcellulose solution is used as an adhesive, 20-200 parts of tea polyphenol, 25-20 parts of hydroxypropyl methylcellulose solution with the mass concentration of 2-8%, and under the conditions of 50-55 ℃ and the flow rate of 2-3 parts/min and the atomization pressure of 0.1-0.5MPa, a fluidized bed top spraying one-step granulator is used for preparing tea polyphenol particles.
The preparation process of the ginger extract emulsion comprises the following steps: sequentially adding 10-25 parts of Arabic gum, 10-25 parts of maltodextrin and 0.5-1.5 parts of sodium stearoyl lactylate into 250-450 parts of water, fully dissolving, adding 50-150 parts of ginger extract, shearing at a high speed of 10-15m/s for 10-20min, and circulating the emulsion in a high-pressure homogenizer for three times under 40-50MPa to obtain the ginger extract emulsion.
The application of the tea polyphenol-gingerol combined particles in preparing the medicine for treating abdominal obesity is that the dosage range of each time is 0.5-5g.
The beneficial effects of the invention are as follows:
according to the tea polyphenol-gingerol combined particles, firstly, the hydroxypropyl methyl cellulose solution is used for wrapping tea polyphenol to form a first layer of coating, the ginger extract can cover the spicy taste after being embedded by the emulsifying agent, meanwhile, the tea polyphenol particles are embedded in the first layer of coating, finally, the targeting wall material top spray coating is used for realizing multi-layer composite coating, and the spicy taste of gingerol and the astringent taste of tea polyphenol can be covered at the same time, so that the adding amount of functional components can be increased, and the complex process and cost waste for separately embedding gingerol and tea polyphenol can be avoided.
The integral water solubility of the tea polyphenol-gingerol combined particles is improved, the bioavailability of gingerol and tea polyphenol is improved, and the weight reduction effect is better than that of the pure gingerol tea polyphenol compound powder.
Further details will be described below with reference to the accompanying drawings.
Drawings
FIG. 1 is a graph showing the effect of each of the administration groups on AMPK activity in the examples of the present invention;
FIG. 2 is a graph showing the effect of each of the administration groups on the expression level of lipolytic enzyme-associated genes in the examples of the present invention;
FIG. 3 is a graph showing the effect of each administration group on the UCP-1 expression level of the brown fat thermogenic gene in the examples of the present invention;
FIG. 4 is a graph showing the effect of each of the administration groups on the expression level of inflammatory factors in the examples of the present invention.
Detailed Description
Further advantages and effects of the present invention will become apparent to those skilled in the art from the disclosure of the present specification, by describing the embodiments of the present invention with specific examples.
The exemplary embodiments of the present invention will now be described with reference to the accompanying drawings, however, the present invention may be embodied in many different forms and is not limited to the examples described herein, which are provided to fully and completely disclose the present invention and fully convey the scope of the invention to those skilled in the art.
Unless otherwise indicated, terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, it will be understood that terms defined in commonly used dictionaries should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense.
Example 1:
the embodiment provides a tea polyphenol-gingerol combined particle, which comprises the following components in parts by weight: 150-300 parts of tea polyphenol particles and 50-100 parts of ginger extract emulsion, wherein the particle size of the polyphenol particles is 100-150 meshes, and the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid.
Wherein the coating liquid is hydroxypropyl methylcellulose-polyethylene glycol mixed solution.
The tea polyphenol particles comprise the following components in parts by weight: 20-200 parts of tea polyphenol and 25-50 parts of hydroxypropyl methyl cellulose solution, wherein the mass concentration of the hydroxypropyl methyl cellulose solution is 2-8%, and the purity of the tea polyphenol is 40-98%.
The ginger extract emulsion comprises the following components in parts by weight: 50-150 parts of ginger extract, 10-25 parts of acacia, 10-25 parts of maltodextrin, 0.5-1.5 parts of sodium stearoyl lactylate and 250-450 parts of water.
The mass ratio of the hydroxypropyl methylcellulose to the polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol mixed solution is 4:1-10:5.
according to the tea polyphenol-gingerol combined particles, firstly, the hydroxypropyl methyl cellulose solution is used for wrapping tea polyphenol to form a first layer of coating, the ginger extract can cover the spicy taste after being embedded by the emulsifying agent, meanwhile, the tea polyphenol particles are embedded in the first layer of coating, finally, the targeting wall material top spray coating is used for realizing multi-layer composite coating, and the spicy taste of gingerol and the astringent taste of tea polyphenol can be covered at the same time, so that the adding amount of functional components can be increased, and the complex process and cost waste for separately embedding gingerol and tea polyphenol can be avoided.
Example 2:
on the basis of the embodiment 1, the embodiment provides a tea polyphenol-gingerol combined particle, which comprises the following components in parts by weight: 150 parts of tea polyphenol particles and 50 parts of ginger extract emulsion, wherein the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid.
The preparation process is as follows:
step 1) preparing tea polyphenol particles: 20 parts of tea polyphenol and 25 parts of 2% hydroxypropyl methylcellulose solution are granulated by a fluidized bed top-spraying one-step granulator. The specific parameters are as follows: the material temperature is 50 ℃, the flow rate is 2 parts/min, the atomization pressure is 0.1MPa, and the tea polyphenol particles are prepared.
Step 2) preparing ginger extract emulsion: 10 parts of Arabic gum, 10 parts of maltodextrin, 0.5 part of sodium stearoyl lactylate and 250 parts of water are fully dissolved, 50 parts of ginger extract is added, high-speed shearing is carried out for 10min at a linear speed of 10m/s, and then the emulsion enters a high-pressure homogenizer for three times of circulation under 40 MPa.
Step 3) 150 parts of tea polyphenol particles as core materials and 50 parts of ginger extract emulsion as coating liquid, wherein the weight of the coating liquid is as followsThe top-spray coating is carried out by a fluidized bed top-spray one-step granulator. The specific parameters are as follows: air volume of 20m 3 And/h, the material temperature is 52 ℃, the flow rate is 3 parts/min, the atomization pressure is 0.1MPa, and the tea polyphenol-gingerol particles are obtained.
Step 4) taking 150 parts of tea polyphenol-gingerol particles as core materials, taking 75 parts of hydroxypropyl methylcellulose-polyethylene glycol solution as coating liquid, and performing top spraying coating to obtain tea polyphenol-gingerol combined particles, wherein the hydroxypropyl methylcellulose and polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol solution are prepared according to the mass ratio of 1:4, adding the solution with the mass concentration of 2%.
Example 3:
on the basis of the embodiment 1, the embodiment provides a tea polyphenol-gingerol combined particle, which comprises the following components in parts by weight: 300 parts of tea polyphenol particles and 100 parts of ginger extract emulsion, wherein the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid.
The preparation process is as follows:
step 1) preparing tea polyphenol particles: 200 parts of tea polyphenol and 50 parts of 8% hydroxypropyl methylcellulose solution are granulated by a fluidized bed top-spraying one-step granulator. The specific parameters are as follows: the material temperature is 55 ℃, the flow rate is 3 parts/min, the atomization pressure is 0.5MPa, and the tea polyphenol particles are prepared.
Step 2) preparing ginger extract emulsion: 25 parts of Arabic gum, 25 parts of maltodextrin, 1.5 parts of sodium stearoyl lactylate and 450 parts of water are fully dissolved, 150 parts of ginger extract is added, high-speed shearing is carried out for 20min at a linear speed of 15m/s, and then the emulsion enters a high-pressure homogenizer for three times of circulation under 50 MPa.
And 3) taking 300 parts of tea polyphenol particles as core materials, taking 100 parts of ginger extract emulsion as coating liquid, and performing top-spraying coating by using a fluidized bed top-spraying one-step granulator. The specific parameters are as follows: air volume of 30m 3 And/h, the temperature of the material is 55 ℃, the flow rate is 5 parts per minute, the atomization pressure is 0.5MPa, and the tea polyphenol-gingerol particles are obtained.
Step 4) 300 parts of tea polyphenol-gingerol particles are used as core materials, 150 parts of hydroxypropyl methylcellulose-polyethylene glycol solution is used as coating liquid, and top spraying coating is carried out to obtain tea polyphenol-gingerol combined particles, wherein the hydroxypropyl methylcellulose and polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol solution are prepared according to the mass ratio of 10:5, adding, wherein the mass concentration of the solution is 5%.
Example 4:
on the basis of the embodiment 1, the embodiment provides a tea polyphenol-gingerol combined particle, which comprises the following components in parts by weight: 225 parts of tea polyphenol particles and 87 parts of ginger extract emulsion, wherein the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid.
The preparation process is as follows:
step 1) preparing tea polyphenol particles: 130 parts of tea polyphenol and 35 parts of 4% hydroxypropyl methylcellulose solution are granulated by a fluidized bed top-spraying one-step granulator. The specific parameters are as follows: the material temperature is 53 ℃, the flow rate is 3 parts/min, the atomization pressure is 0.3MPa, and the tea polyphenol particles are prepared.
Step 2) preparing ginger extract emulsion: 20 parts of Arabic gum, 20 parts of maltodextrin, 1 part of sodium stearoyl lactate and 300 parts of water are fully dissolved, 100 parts of ginger extract is added, high-speed shearing is carried out for 15min at a linear speed of 12m/s, and then the emulsion enters a high-pressure homogenizer for three times of circulation under 45 MPa.
Step 3) taking 225 parts of tea polyphenol particles as core materials, taking 87 parts of ginger extract emulsion as coating liquid, and performing top-spraying coating by using a fluidized bed top-spraying one-step granulator. The specific parameters are as follows: air volume 25m 3 And/h, the material temperature is 54 ℃, the flow rate is 4 parts/min, the atomization pressure is 0.3MPa, and the tea polyphenol-gingerol particles are obtained.
Step 4) taking 245 parts of tea polyphenol-gingerol particles as core materials, taking 96 parts of hydroxypropyl methylcellulose-polyethylene glycol solution as coating liquid, and performing top spraying coating to obtain tea polyphenol-gingerol combined particles, wherein the hydroxypropyl methylcellulose and polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol solution are prepared according to a mass ratio of 5:1, the mass concentration of the solution is 3%.
1. Study of the influence of the tea polyphenol-gingerol Combined particles prepared in the embodiment on the body temperature variation of mice
1. Experimental animal
SPF-class Kunming mice 120, male, body weight 18-22 g, were offered by the university of traffic animal laboratory center of Western An. The raising environment temperature of the mice is 22+/-1 ℃, the relative humidity is 50+/-10%, and the light and dark period is 12:12.
2. experimental method
80 mice of 5 weeks of age were subjected to three days of adaptive rearing. During the period, sufficient water and normal diet are given, the water solvent gastric lavage treatment is carried out once a day, and the anal temperature of the mice is measured after the administration, so that the mice adapt to the anal temperature measurement operation, and the temperature rise caused by the stress of the mice due to the anal temperature measurement is reduced. After three days, all mice were randomly divided into 4 groups, a blank group, a gingerol-tea polyphenol composite powder group (gingerol and tea polyphenol direct mixture), a tea polyphenol-gingerol combined particle group, and a positive control group (capsaicin), 20 mice each, each group was respectively administered by gastric lavage, the anus temperature of the mice was measured at three time points of 30, 60, 90 minutes of administration, and the anus temperature change value was recorded.
3. Experimental results
Table 1 body temperature values of mice before and after administration
Note that: * P <0.05, P <0.01, P <0.005
TABLE 2 body temperature variation values of mice before and after administration
The body temperature rise can intuitively reflect the heat production effect of brown fat, and the body temperature rise can improve the overall metabolism rate, further promote the energy consumption and facilitate the fat reduction effect. As can be seen from tables 1 and 2, the gingerol-tea polyphenol composite powder group and the tea polyphenol-gingerol composite particles all can raise the body temperature of mice after 60 minutes of administration compared with the blank group. And when the administration is carried out for 90min, the temperature rise value of the mice in the tea polyphenol-gingerol combined particle group is larger than that of the mice in the positive control group (capsaicin) and the gingerol-tea polyphenol composite powder group. In conclusion, the tea polyphenol-gingerol combined particles can obviously improve the body temperature of mice, the temperature rise time is longer, and the effect is better than that of gingerol-tea polyphenol composite powder.
2. The influence of the tea polyphenol-gingerol combined particles prepared in the embodiment on the fatty acid metabolism and the fat cell factor of mice is studied
1. Experimental method
36 male rats with 3 weeks of age were kept at 22+ -1deg.C, 50+ -10% relative humidity, 12 light-dark cycle: 12 in the animal house. After one week of adaptation, the animals were randomly divided into 3 groups (n=12) which are respectively a high-fat diet group, a high-fat diet+gingerol-tea polyphenol composite powder group, a high-fat diet+gingerol-tea polyphenol microparticle group, initial weight values were recorded, feeding was performed for 10 weeks, and after the experiment was completed, final weight values were recorded, and the results are shown in table 3; and the rats were fasted for 10 hours, treated with ease, and serum and adipose tissues were collected. AMPK activity was assessed using AMPK kinase assay kit. UCP-1, HSL, ATGL, TNF- α, IL-6, NF-KB and adiponectin mRNA levels were assayed using the Real time-PCR method.
Table 3 weight change after 10 weeks of high fat diet feeding for each dosing group
Throughout the feeding period, rats gained weight by 174.1±14.9g on a high fat diet; high fat diet + gingerol-tea polyphenol compound powder group, weight gain of rats is 140.0+ -15.2 g; high fat diet + tea polyphenol-gingerol combination pellet group, rats weight gain 112.3 + -11.6 g. As can be seen from table 3, there were statistically significant differences between the high-fat diet, the high-fat diet + gingerol-tea polyphenol composite powder group, the high-fat diet + tea polyphenol-gingerol composite pellet group. The high-fat diet and tea polyphenol-gingerol combined particle group can obviously inhibit and relieve obesity of mice caused by high-fat diet, and the effect is better than that of the high-fat diet and gingerol-tea polyphenol composite powder.
AMPK is a key molecule for regulation of fat metabolism, which can promote lipolysis and beta-oxidation of fatty acids. Compared with a high-fat diet group, the activity of the AMPK of the gingerol-tea polyphenol composite powder group is increased by 45.8%, the activity of the AMPK of the tea polyphenol-gingerol combined particle group is increased by 87.5%, and the activity enhancement effect of the tea polyphenol-gingerol combined particle on the AMPK is better than that of the gingerol-tea polyphenol composite powder. See fig. 1.
The activity of lipolytic enzyme may reflect the degree of lipolytic activity. Compared with a high-fat diet group, the expression level of the Hormone Sensitive Lipase (HSL) of the gingerol-tea polyphenol composite powder group is increased by 1 time, and the expression level of the Hormone Sensitive Lipase (HSL) of the tea polyphenol-gingerol composite microparticle group is increased by 2 times. The expression level of triglyceride lipase (ATGL) of the gingerol-tea polyphenol composite powder group is increased by 5 times, and the expression level of mRNA of the tea polyphenol-gingerol composite particle group is increased by 6.5 times, which is shown in figure 2. Therefore, the tea polyphenol-gingerol combined particle group can increase the activity of lipolytic enzyme, and the effect is better than that of gingerol-tea polyphenol composite powder group.
UCP-1 is uncoupling protein, and can reflect the heat generation condition of brown fat. Compared with a high-fat diet group, the expression of UCP-1 mRAN can be obviously improved by both the gingerol-tea polyphenol composite powder group and the tea polyphenol-gingerol composite particle group. Wherein the UCP-1 mRAN expression level in the gingerol-tea polyphenol composite powder is increased by 1.8 times, and the UCP-1 mRAN expression level of the tea polyphenol-gingerol combined micro-particle group is increased by 2.6 times, as shown in figure 3. Therefore, the tea polyphenol-gingerol combined particle group can obviously promote the UCP-1 expression quantity, and the effect is better than that of the gingerol-tea polyphenol composite powder group.
Interleukin 6 (IL-6), tumor necrosis factor (TNF-alpha) and nuclear factor kB (NF-kB) are pro-inflammatory cytokines in adipocytes, and reflect inflammatory states. High levels of inflammatory factors have been found to counteract fatty acid metabolism, inhibit catabolism of fatty acids, and exacerbate obesity. From the figure, it is clear that the mice can develop inflammatory responses on a long-term high-fat diet. The tea polyphenol-gingerol combined particles can obviously reduce the expression of each inflammatory factor. And the anti-inflammatory effect of the tea polyphenol-gingerol combined particles is better than that of gingerol-tea polyphenol composite powder. As shown in fig. 4.
According to experiments, the tea polyphenol-gingerol combined particles can strengthen fatty acid metabolism by promoting brown fat thermogenesis and white fat beta-oxidation, regulate and control fat cell secretion factors (correct adiponectin up-regulation, inflammatory factor down-regulation and reduce insulin resistance) so that the secretion concentration of the tea polyphenol-gingerol combined particles tends to be in a healthy and normal state of an organism, remarkably improve metabolic syndrome and finally realize the fat reduction effect of abdominal obesity. And the effect of the tea polyphenol-gingerol combined particles is obviously better than that of gingerol-tea polyphenol composite powder.
3. The influence of the tea polyphenol-gingerol combined particles prepared in the embodiment on the weight and waistline of the adult is studied
Test population: overweight and obese women; number of test persons: 50 persons; test period: 4 weeks of
Grouping: the placebo group took resistant dextrin capsules daily; the experimental group took the tea polyphenol-gingerol combination particles prepared in example 4 every day. During the test period, both the experimental and placebo groups were on a normal diet. The experimental results are shown in Table 4.
TABLE 4 test of effects of gingerol-tea polyphenol particles on human body weight loss and waist circumference variation
As can be seen from the results in Table 4, after taking the tea polyphenol-gingerol combination particles for 4 weeks, the subjects in the experimental group had a weight loss of-1.9.+ -. 1.1kg, a waist circumference loss of-3.2.+ -. 0.5cm, and a very significant difference from the placebo group. The tea polyphenol-gingerol combined particles have good weight and waistline reducing effects on abdominal obesity.
4. Study of the influence of the tea polyphenol-gingerol Combined particles prepared in the embodiment on the body temperature variation of adults
Test population: overweight and obese adult
Number of test persons: 20 people
Test period: for 2 hours
The test mode is as follows: before taking the product and after taking the product for 2 hours, the abdomen temperature was measured by a body temperature gun, and the body temperature change value was recorded, and the results are shown in Table 5.
Table 5 tea polyphenols-gingerol combined particles for changing human body temperature
The increase of body temperature is an intuitive expression of the heat generating effect of brown fat, is more beneficial to improving the basic metabolic rate of the organism and accelerating the fat reduction. As can be seen from Table 5, after taking the ginger tea polyphenol-gingerol combination particles, the body temperature can be raised by 0.2-0.6 ℃ within 2 hours, and the average temperature can be raised by 0.4 ℃.
The mechanism of the invention:
gingerol can activate the sympathetic nerve of the organism through TRPV1 channel, under the higher sympathetic nerve effect, can promote the secretion of norepinephrine, further activate the brown fat cell surface beta 3-AR receptor, promote the decomposition of lipid droplets in brown fat, increase the expression of thermogenesis key factor uncoupling protein UCP-1, promote fatty acid metabolism and release in a thermal energy form. The tea polyphenol can inhibit catechol-O-methyltransferase (COMT) activity, so that the reduction of norepinephrine is reduced, and the combination of ginger powder and green tea powder can keep the norepinephrine in a high concentration state for a long time, so that the beta 3-AR receptor on the surface of brown fat cells is in an activated state for a long time, and the brown fat heat production can be continuously promoted.
The gingerol and tea polyphenol act on white fat beta-oxidation together, and both the gingerol and the tea polyphenol can promote phosphorylation of an AMPK pathway of an organism, improve the expression level of lipolytic enzymes (hormone sensitive lipase HSL and triglyceride lipase ATGL), promote the decomposition of triglyceride into fatty acid, transfer the fatty acid into mitochondria and promote beta-oxidation of the fatty acid.
The gingerol and tea polyphenol complement and regulate and control the secretion factors of fat cells, and the gingerol can inhibit the NF-KB verification path. The tea polyphenol can inhibit the expression of IL-6 and TNF-alpha inflammatory factors, promote the expression of adiponectin, and slow down insulin resistance. The combination of gingerol and tea polyphenol can make the concentration ratio of each fat cell secretion factor be normalized.
The foregoing examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention, and all designs that are the same or similar to the present invention are within the scope of the present invention.
Claims (6)
1. A tea polyphenol-gingerol combined particle is characterized in that: comprises the following components in parts by weight: 150-300 parts of tea polyphenol particles and 50-100 parts of ginger extract emulsion, wherein the tea polyphenol particles and the ginger extract emulsion are mixed and then coated by coating liquid;
the tea polyphenol particles comprise the following components in parts by weight: 20-200 parts of tea polyphenol and 25-50 parts of hydroxypropyl methylcellulose solution, wherein the mass concentration of the hydroxypropyl methylcellulose solution is 2-8%, and the purity of the tea polyphenol is 40-98%;
the coating liquid is hydroxypropyl methylcellulose-polyethylene glycol mixed solution; the ginger extract emulsion comprises the following components in parts by weight: 50-150 parts of ginger extract, 10-25 parts of acacia, 10-25 parts of maltodextrin, 0.5-1.5 parts of sodium stearoyl lactylate and 250-450 parts of water; the ginger extract contains 5% -60% of gingerol.
2. The tea polyphenol-gingerol combination granule as claimed in claim 1, wherein: the mass ratio of the hydroxypropyl methylcellulose to the polyethylene glycol in the hydroxypropyl methylcellulose-polyethylene glycol mixed solution is 4:1-10:5.
3. the method for preparing the tea polyphenol-gingerol combined particles according to any one of claims 1 to 2, comprising the steps of:
step 1) preparing tea polyphenol particles;
step 2) preparing ginger extract emulsion;
step 3) taking the tea polyphenol particles as a core material, taking ginger extract emulsion as coating liquid, and performing bottom spraying coating to obtain the tea polyphenol-gingerol particles;
and 4) taking the tea polyphenol-gingerol particles as core materials, taking a hydroxypropyl methyl cellulose-polyethylene glycol mixed solution as coating liquid, and performing top spraying coating to obtain the tea polyphenol-gingerol combined particles.
4. A method for preparing the tea polyphenol-gingerol combined particles according to claim 3, wherein: the preparation process of the tea polyphenol particles comprises the following steps: tea polyphenol is used as a core material, hydroxypropyl methylcellulose solution is used as an adhesive, 20-200 parts of tea polyphenol, 25-20 parts of hydroxypropyl methylcellulose solution with the mass concentration of 2-8%, and under the conditions of 50-55 ℃ and the flow rate of 2-3 parts/min and the atomization pressure of 0.1-0.5MPa, a fluidized bed top spraying one-step granulator is used for preparing tea polyphenol particles.
5. A method for preparing the tea polyphenol-gingerol combined particles according to claim 3, wherein: the preparation process of the ginger extract emulsion comprises the following steps: sequentially adding 10-25 parts of Arabic gum, 10-25 parts of maltodextrin and 0.5-1.5 parts of sodium stearoyl lactylate into 250-450 parts of water, fully dissolving, adding 50-150 parts of ginger extract, shearing at a high speed of 10-15m/s for 10-20min, and circulating the emulsion in a high-pressure homogenizer for three times under 40-50MPa to obtain the ginger extract emulsion.
6. Use of a tea polyphenol-gingerol combination microparticle according to any of claims 1-2, characterized in that: is used for preparing abdominal weight-reducing product, and the dosage is 0.5-5g each time.
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