CN113943795B - Probe set for detecting noise-related deafness gene and kit thereof - Google Patents
Probe set for detecting noise-related deafness gene and kit thereof Download PDFInfo
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Abstract
The invention provides a probe set for detecting noise-related deafness genes, which comprises probes capable of simultaneously and specifically capturing 52 noise-related deafness genes, and also provides a kit for detecting the probe set for detecting the noise-related deafness genes, comprising the probe set for detecting the noise-related deafness genes, an enrichment buffer solution, a hybridization buffer solution, a binding buffer solution, a rinsing solution 1, a rinsing solution 2, a NaOH solution, a Tris-HCl buffer solution, a PCR reaction solution and a TE buffer solution. The invention designs specific capture probes for 52 noise-related deafness genes and performs exon enrichment for each gene, and can be used for preventing and diagnosing noise-related deafness, selecting relevant industrial staff and judging noise-related deafness.
Description
Technical Field
The invention belongs to the technical field of genetic engineering, and particularly relates to a probe set for detecting noise-related deafness genes and a kit thereof.
Background
Noise deafness is an acquired progressive sensorineural hearing impairment caused by exposure to noise environments such as industry, military, entertainment and the like, early stage is manifested by hearing fatigue, can be gradually recovered after leaving the noise environment, is difficult to recover after a long time, and finally leads to sensorineural deafness. Noise-induced deafness is a occupational disease that is commonly occurring worldwide, severely affecting public health. It is currently widely accepted that noise-induced deafness is the result of environment-genetic interaction, individuals are exposed to the same noise environment, the occurrence or harm degree of hearing impairment is different, and it is suggested that individual genetic susceptibility plays an important role in noise-induced deafness, and the genetic susceptibility is mainly dependent on individual genetic susceptibility genes. Noise-induced deafness susceptibility genes are mainly concentrated on oxidative stress genes, monogenic deafness genes, potassium ion circulating channel genes, heat shock proteins, cadherin pathway genes, notch signaling pathway genes, and apoptosis signaling pathway genes.
For detection of pathogenic/susceptibility genes, the method of designing primers one by one, PCR amplification, sanger sequencing and alignment with standard sequences is time-consuming, laborious and costly, and poses a great obstacle to genetic counseling and the resolution of many related problems.
The high-throughput sequencing technology has the advantages of rapidness, accuracy and low cost, can detect various types of mutation of a plurality of genes at the same time, has been widely applied to etiology detection and molecular genetic diagnosis of genetic defects, but no high-throughput probe, chip or kit special for susceptibility gene detection of noise deafness exists in the market at present, so that practitioners in the related fields lack effective prevention and control consciousness, and bring heavy psychological and economic burden to patients and families thereof.
In conclusion, the high-efficiency, accurate and rapid noise deafness related gene detection kit is developed and is used for susceptibility gene screening, and has great significance in the aspects of noise related professional screening, professional protection and the like.
Disclosure of Invention
The invention aims at solving the technical problems of the prior art and provides a probe set for detecting noise-related deafness genes and a kit thereof, wherein the probe set and the kit thereof are used for designing specific capture probes for 52 noise-related deafness genes and carrying out exon enrichment for each gene, and can be used for preventing and diagnosing noise-related deafness and for selecting relevant industrial staff and judging noise-related deafness.
In order to solve the technical problems, the invention adopts the following technical scheme: a probe set for detecting noise-related deafness genes, the probe set comprising probes capable of simultaneously specifically capturing the following 52 noise-related deafness susceptibility genes: CASP9, GJB4, KCNQ4, ITGA8, PCDH15, CDH23, KCNQ1, CAT, CASP4, CASP1, HOTAIR, GJB2, APEX1, XYLT1, DNMT1, CASP14, DNMT3A, GABPA (also referred to as NRF 2), SOD1, GRM7, ATP2B2, CASP3, POU4F3, HSPA1A, HSPA1B, GSTT1, FOXO3, EYA4, SOD2, GSDME (also referred to as DFNA 5), PON2, GRHL2, notch1, GJB1, KCNE1, GSTP1, HSPA1L, CASP, CASP6, CASP8, CASP10, nmkca 1, MYH14, OGG1, IL6, WFS1, CLCNKB, IL1B, IL4R, XPO, GSTM1, nj10.
7 oxidative stress pathway genes: CAT, GSTT1, PON2, SOD1, GSTM1, GSTP1 and SOD2;
the continuous strong noise stimulation can cause ischemia and hypoxia of the inner ear, the activities of antioxidant enzymes and free radical scavengers are reduced, oxidation injury signals are generated, at the moment, the balance of oxidation and antioxidant systems in the body is unbalanced, oxidation stress is caused, a large amount of active oxygen (reactive oxygen species, ROS) is generated, and the ROS products have extremely strong function of destroying the internal connection of molecules, so that cochlear cells or tissues can be damaged, and hearing loss is caused.
7 potassium ion circulation pathway genes: GJB1, GJB2, GJB4, KCNE1, KCNQ4, KCNJ10;
the intra-cochlear lymph fluid is rich in potassium ions, participates in maintaining a high potential of endolymph, transports most sodium ions, and depolarizes at the top of cells upon hair cell electrical transduction. Abnormal potassium ion circulation passage can cause abnormal potential of the handbag and deafness.
3 heat shock protein pathway genes: HSPA1A, HSPA1B, HSPA L;
heat shock proteins are functional related proteins, and can be over expressed in the inner ear and other parts caused by stimulation such as physiological stress noise and the like.
2 cadherin pathway genes: CDH23, PCDH15;
cadherin is a calcium ion-dependent cell adhesion glycoprotein and plays an important role in cell recognition, migration and tissue differentiation of embryonic development and in the constitution of adult tissue organs. Polymorphisms in both gene expression are closely related to NIHL susceptibility.
9 apoptosis signaling pathway genes: CASP1, CASP3, CASP4, CASP9, CASP14, CASP5, CASP6, CASP8, and CASP10;
cysteine-containing aspartic proteases (caspases) are a group of proteases with similar structures found in the cytoplasm, closely related to changes in morphological characteristics of apoptosis, and involved in the growth and differentiation of cells. Noise exposure can accelerate Caspase protease expression in the inner ear and thereby exacerbate apoptosis.
1 Notch signaling pathway gene: notch1;
notch signals are highly conserved, can participate in multiple vital activities such as cell proliferation and differentiation, and maintain internal environment dynamic balance. After the occurrence of the noise-induced deafness, the cochlear sensory hair cells are largely damaged or dead and cannot be regenerated.
23 NIHL-related monogenes: KCNMA1, MYH14, OGG1, IL6, WFS1, CLCNKB, IL1B, IL, R, XPO5, APEX1, GSDME (DFNA 5), DNMT1, DNMT3A, EYA4, FOXO3, GRHL2, ITGA8, POU4F3, GRM7, HOTAIR, ATP2B2, XYLT1, and gaba (NRF 2).
Preferably, the probe set for capturing CASP9 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown in SEQ ID No. 1-9;
the probe set for capturing the GJB4 gene comprises 35 oligonucleotide probes, and the base sequences of the 35 oligonucleotide probes are shown as SEQ ID No. 10-44;
the probe set for capturing KCNQ4 genes comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 45-48;
the probe set for capturing the ITGA8 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 49-50;
the probe set for capturing the PCDH15 gene comprises 48 oligonucleotide probes, and the base sequences of the 48 oligonucleotide probes are shown as SEQ ID No. 51-98;
The probe set for capturing CDH23 gene comprises 2 oligonucleotide probes, wherein the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 99-100;
the probe set for capturing KCNQ1 gene comprises 50 oligonucleotide probes, and the base sequences of the 50 oligonucleotide probes are shown as SEQ ID No. 101-150;
the probe set for capturing CAT genes comprises 42 oligonucleotide probes, and the base sequences of the 42 oligonucleotide probes are shown as SEQ ID Nos. 151-192;
the probe set for capturing CASP4 gene comprises 14 oligonucleotide probes, and the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 193-206;
the probe set for capturing the CASP1 gene comprises 5 oligonucleotide probes, and the base sequence of the 5 oligonucleotide probes is shown as SEQ ID No. 207-211;
the probe set for capturing the HOTAIR gene comprises 6 oligonucleotide probes, and the base sequences of the 6 oligonucleotide probes are shown as SEQ ID Nos. 212-217;
the probe set for capturing the GJB2 gene comprises 34 oligonucleotide probes, and the base sequences of the 34 oligonucleotide probes are shown as SEQ ID No. 218-251;
the probe set for capturing the APEX1 gene comprises 2 oligonucleotide probes, and the base sequences of the 2 oligonucleotide probes are shown as SEQ ID Nos. 252-253;
The probe set for capturing the XYLT1 gene comprises 39 oligonucleotide probes, and the base sequences of the 39 oligonucleotide probes are shown as SEQ ID No. 254-292;
the probe set for capturing DNMT1 gene comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 293-300;
the probe set for capturing the CASP14 gene comprises 10 oligonucleotide probes, wherein the base sequences of the 10 oligonucleotide probes are shown as SEQ ID Nos. 301-310;
the probe set for capturing MYH14 genes comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 311-318;
the probe set for capturing DNMT3A gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 319-325;
the probe set for capturing GABPA (NRF 2) gene comprises 13 oligonucleotide probes, and the base sequences of the 13 oligonucleotide probes are shown as SEQ ID Nos. 326-338;
the probe set for capturing SOD1 gene comprises 20 oligonucleotide probes, and the base sequences of the 20 oligonucleotide probes are shown in SEQ ID No. 339-358;
the probe set for capturing GRM7 gene comprises 28 oligonucleotide probes, and the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 359-386;
The probe set for capturing the ATP2B2 gene comprises 16 oligonucleotide probes, and the base sequence of the 16 oligonucleotide probes is shown as SEQ ID No. 387-402;
the probe set for capturing the CASP3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 403-406;
the probe set for capturing the POU4F3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID Nos. 407-410;
the probe set for capturing the HSPA1A gene comprises 79 oligonucleotide probes, and the base sequences of the 79 oligonucleotide probes are shown as SEQ ID No. 411-489;
the probe set for capturing the HSPA1B gene comprises 2 oligonucleotide probes, wherein the base sequences of the 2 oligonucleotide probes are shown as SEQ ID No. 490-491;
the probe group for capturing GSTT1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 492-493;
the probe set for capturing the FOXO3 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown as SEQ ID Nos. 494-502;
the probe set for capturing EYA4 genes comprises 27 oligonucleotide probes, and the base sequences of the 27 oligonucleotide probes are shown as SEQ ID Nos. 503-529;
The probe set for capturing SOD2 gene comprises 20 oligonucleotide probes, and the base sequence of the 20 oligonucleotide probes is shown as SEQ ID No. 530-549;
the probe set for capturing GSDME (DFNA 5) gene comprises 2 oligonucleotide probes, wherein the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 550-551;
the probe set for capturing the PON2 gene comprises 28 oligonucleotide probes, wherein the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 552-579;
the probe set for capturing GRHL2 genes comprises 40 oligonucleotide probes, and the base sequence of the 40 oligonucleotide probes is shown as SEQ ID No. 580-619;
the probe set for capturing the Notch1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 620-621;
the probe set for capturing the GJB1 gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 622-628;
the probe set for capturing KCNE1 gene comprises 14 oligonucleotide probes, wherein the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 629-642;
the probe set for capturing GSTP1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 643;
The probe set for capturing the HSPA1L gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 644;
the probe set for capturing the CASP5 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 645;
the probe set for capturing CASP6 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 646;
the probe set for capturing CASP8 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 647;
the probe set for capturing the CASP10 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 648;
the probe set for capturing KCNMA1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 649;
the probe set for capturing MYH14 genes comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 650;
the probe set for capturing the OGG1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 651;
The probe set for capturing IL6 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 652;
the probe set for capturing the WFS1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 653;
the probe set for capturing the CLCNKB gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 654;
the probe set for capturing the IL1B gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 655;
the probe set for capturing IL4R gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 656;
the probe set for capturing XPO5 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 657;
the probe set for capturing GSTM1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 658;
the probe set for capturing KCNJ10 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 659.
The invention also provides a kit of the probe set for detecting the noise-related deafness gene, which comprises:
(1) A probe set for detecting a noise-related deafness gene;
(2) An enrichment buffer, wherein the enrichment buffer contains a primer, human cot-1DNA and salmon DNA;
(3) Hybridization buffer containing SSPE buffer, denhardt solution, EDTA and SDS;
the hybridization buffer solution is composed of the following raw materials in parts by weight: 5-fold concentration of SSPE buffer, 5-fold concentration of Denhardt solution, 5mM EDTA, 0.1% SDS;
(4) A binding buffer solution, wherein the binding buffer solution contains NaCl, tris-HCl and EDTA;
the 1-fold concentration of binding buffer consisted of the following raw materials: 1M NaCl, 10mM Tris-HCl (pH 7.5), 1mM EDTA;
(5) A rinse solution 1, wherein the rinse solution 1 contains SSC and SDS;
the rinse liquid 1 is composed of the following raw materials in parts by weight: SSS, 0.1% sds at 1-fold concentration;
(6) A rinse solution 2, wherein the rinse solution 2 contains SSC and SDS;
the rinse liquid 1 is composed of the following raw materials in parts by weight: SSS, 0.1% sds at 1-fold concentration;
the SSS preparation method with 1-time concentration comprises the following steps: 175g of NaCl, 88g of trisodium citrate, adjusting the pH to 7.4 and dH 2 O constant volume to 1 liter;
(7) NaOH solution; the concentration of the NaOH solution is 0.1M;
(8) Tris-HCl buffer, pH 7.5;
(9) A PCR reaction solution containing dNTPs, a primer (the nucleotide sequence of the forward primer P5 is AATGATACGGCGACCACCGA G, the nucleotide sequence of the reverse primer P7 is CAAGCAGAAGACGGCATACG A, and the middle is thio-modified), a Phusion buffer solution, a hotspot Phusion enzyme, DMSO and dH 2 O；
The PCR reaction solution consists of the following raw materials in percentage by weight: 2. Mu.L dNTPs (10 mM each), 0.5. Mu.L primer, 20. Mu.L 5-fold concentration of Phusion buffer, 1. Mu. L Hotstart Phusion enzyme, 5. Mu.L DMSO, 51. Mu.L dH 2 O；
(10) TE buffer;
the TE buffer solution is prepared from the following raw materials in parts by weight: 10mM Tris-HCl,1mM EDTA, pH8.0, water to 500ml.
Conventional reagents used: SSPE buffer at 20-fold concentration, available from ameresco company; denhardt's solution at 50-fold concentration, available from USB company; EDTA solution: 0.5M, pH8.0, available from Meditech; phusion buffer, 5-fold strength, available from New England Biolab; the hotspot Phusion enzyme was purchased from New England Biolabs.
Compared with the prior art, the invention has the following advantages:
The invention designs specific capture probes for 52 noise-related deafness genes and performs exon enrichment for each gene, and can be used for preventing and diagnosing noise-related deafness, selecting relevant industrial staff and judging noise-related deafness. The invention adopts a second generation sequencing method, and has high flux; high cost performance and can be used for large-scale parallel sequencing.
The present invention will be described in further detail with reference to examples.
Detailed Description
Example 1
A probe set for detecting noise-related deafness genes of the present embodiment, comprising probes capable of simultaneously and specifically capturing the following 52 noise-related deafness genes: CASP9, GJB4, KCNQ4, ITGA8, PCDH15, CDH23, KCNQ1, CAT, CASP4, CASP1, HOTAIR, GJB2, APEX1, XYLT1, DNMT1, CASP14, DNMT3A, GABPA (also referred to as NRF 2), SOD1, GRM7, ATP2B2, CASP3, POU4F3, HSPA1A, HSPA1B, GSTT1, FOXO3, EYA4, SOD2, GSDME (also referred to as DFNA 5), PON2, GRHL2, notch1, GJB1, KCNE1, GSTP1, HSPA1L, CASP, CASP6, CASP8, CASP10, nmkca 1, MYH14, OGG1, IL6, WFS1, CLCNKB, IL1B, IL4R, XPO, GSTM1, nj10.
7 oxidative stress pathway genes: CAT, GSTT1, PON2, SOD1, GSTM1, GSTP1 and SOD2;
the continuous strong noise stimulation can cause ischemia and hypoxia of the inner ear, the activities of antioxidant enzymes and free radical scavengers are reduced, oxidation injury signals are generated, at the moment, the balance of oxidation and antioxidant systems in the body is unbalanced, oxidation stress is caused, a large amount of active oxygen (reactive oxygen species, ROS) is generated, and the ROS products have extremely strong function of destroying the internal connection of molecules, so that cochlear cells or tissues can be damaged, and hearing loss is caused.
7 potassium ion circulation pathway genes: GJB1, GJB2, GJB4, KCNE1, KCNQ4, KCNJ10;
the intra-cochlear lymph fluid is rich in potassium ions, participates in maintaining a high potential of endolymph, transports most sodium ions, and depolarizes at the top of cells upon hair cell electrical transduction. Abnormal potassium ion circulation passage can cause abnormal potential of the handbag and deafness.
3 heat shock protein pathway genes: HSPA1A, HSPA1B, HSPA L;
heat shock proteins are functional related proteins, and can be over expressed in the inner ear and other parts caused by stimulation such as physiological stress noise and the like.
2 cadherin pathway genes: CDH23, PCDH15;
Cadherin is a calcium ion-dependent cell adhesion glycoprotein and plays an important role in cell recognition, migration and tissue differentiation of embryonic development and in the constitution of adult tissue organs. Polymorphisms in both gene expression are closely related to NIHL susceptibility.
9 apoptosis signaling pathway genes: CASP1, CASP3, CASP4, CASP9, CASP14, CASP5, CASP6, CASP8, and CASP10;
cysteine-containing aspartic proteases (caspases) are a group of proteases with similar structures found in the cytoplasm, closely related to changes in morphological characteristics of apoptosis, and involved in the growth and differentiation of cells. Noise exposure can accelerate Caspase protease expression in the inner ear and thereby exacerbate apoptosis.
1 Notch signaling pathway gene: notch1;
notch signals are highly conserved, can participate in multiple vital activities such as cell proliferation and differentiation, and maintain internal environment dynamic balance. After the occurrence of the noise-induced deafness, the cochlear sensory hair cells are largely damaged or dead and cannot be regenerated.
23 NIHL-related monogenes: KCNMA1, MYH14, OGG1, IL6, WFS1, CLCNKB, IL1B, IL R, XPO5, APEX1, GSDME (DFNA 5), DNMT1, DNMT3A, EYA4, FOXO3, GRHL2, ITGA8, POU4F3, GRM7, HOTAIR, ATP2B2, XYLT1, and gaba (NRF 2);
The probe set for capturing the CASP9 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown in SEQ ID No. 1-9;
the probe set for capturing the GJB4 gene comprises 35 oligonucleotide probes, and the base sequences of the 35 oligonucleotide probes are shown as SEQ ID No. 10-44;
the probe set for capturing KCNQ4 genes comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 45-48;
the probe set for capturing the ITGA8 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 49-50;
the probe set for capturing the PCDH15 gene comprises 48 oligonucleotide probes, and the base sequences of the 48 oligonucleotide probes are shown as SEQ ID No. 51-98;
the probe set for capturing CDH23 gene comprises 2 oligonucleotide probes, wherein the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 99-100;
the probe set for capturing KCNQ1 gene comprises 50 oligonucleotide probes, and the base sequences of the 50 oligonucleotide probes are shown as SEQ ID No. 101-150;
the probe set for capturing CAT genes comprises 42 oligonucleotide probes, and the base sequences of the 42 oligonucleotide probes are shown as SEQ ID Nos. 151-192;
The probe set for capturing CASP4 gene comprises 14 oligonucleotide probes, and the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 193-206;
the probe set for capturing the CASP1 gene comprises 5 oligonucleotide probes, and the base sequence of the 5 oligonucleotide probes is shown as SEQ ID No. 207-211;
the probe set for capturing the HOTAIR gene comprises 6 oligonucleotide probes, and the base sequences of the 6 oligonucleotide probes are shown as SEQ ID Nos. 212-217;
the probe set for capturing the GJB2 gene comprises 34 oligonucleotide probes, and the base sequences of the 34 oligonucleotide probes are shown as SEQ ID No. 218-251;
the probe set for capturing the APEX1 gene comprises 2 oligonucleotide probes, and the base sequences of the 2 oligonucleotide probes are shown as SEQ ID Nos. 252-253;
the probe set for capturing the XYLT1 gene comprises 39 oligonucleotide probes, and the base sequences of the 39 oligonucleotide probes are shown as SEQ ID No. 254-292;
the probe set for capturing DNMT1 gene comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 293-300;
the probe set for capturing the CASP14 gene comprises 10 oligonucleotide probes, wherein the base sequences of the 10 oligonucleotide probes are shown as SEQ ID Nos. 301-310;
The probe set for capturing MYH14 genes comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 311-318;
the probe set for capturing DNMT3A gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 319-325;
the probe set for capturing GABPA (NRF 2) gene comprises 13 oligonucleotide probes, and the base sequences of the 13 oligonucleotide probes are shown as SEQ ID Nos. 326-338;
the probe set for capturing SOD1 gene comprises 20 oligonucleotide probes, and the base sequences of the 20 oligonucleotide probes are shown in SEQ ID No. 339-358;
the probe set for capturing GRM7 gene comprises 28 oligonucleotide probes, and the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 359-386;
the probe set for capturing the ATP2B2 gene comprises 16 oligonucleotide probes, and the base sequence of the 16 oligonucleotide probes is shown as SEQ ID No. 387-402;
the probe set for capturing the CASP3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 403-406;
the probe set for capturing the POU4F3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID Nos. 407-410;
The probe set for capturing the HSPA1A gene comprises 79 oligonucleotide probes, and the base sequences of the 79 oligonucleotide probes are shown as SEQ ID No. 411-489;
the probe set for capturing the HSPA1B gene comprises 2 oligonucleotide probes, wherein the base sequences of the 2 oligonucleotide probes are shown as SEQ ID No. 490-491;
the probe group for capturing GSTT1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 492-493;
the probe set for capturing the FOXO3 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown as SEQ ID Nos. 494-502;
the probe set for capturing EYA4 genes comprises 27 oligonucleotide probes, and the base sequences of the 27 oligonucleotide probes are shown as SEQ ID Nos. 503-529;
the probe set for capturing SOD2 gene comprises 20 oligonucleotide probes, and the base sequence of the 20 oligonucleotide probes is shown as SEQ ID No. 530-549;
the probe set for capturing GSDME (DFNA 5) gene comprises 2 oligonucleotide probes, wherein the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 550-551;
the probe set for capturing the PON2 gene comprises 28 oligonucleotide probes, wherein the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 552-579;
The probe set for capturing GRHL2 genes comprises 40 oligonucleotide probes, and the base sequence of the 40 oligonucleotide probes is shown as SEQ ID No. 580-619;
the probe set for capturing the Notch1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 620-621;
the probe set for capturing the GJB1 gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 622-628;
the probe set for capturing KCNE1 gene comprises 14 oligonucleotide probes, wherein the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 629-642;
the probe set for capturing GSTP1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 643;
the probe set for capturing the HSPA1L gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 644;
the probe set for capturing the CASP5 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 645;
the probe set for capturing CASP6 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 646;
The probe set for capturing CASP8 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 647;
the probe set for capturing the CASP10 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 648;
the probe set for capturing KCNMA1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 649;
the probe set for capturing MYH14 genes comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 650;
the probe set for capturing the OGG1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 651;
the probe set for capturing IL6 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 652;
the probe set for capturing the WFS1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 653;
the probe set for capturing the CLCNKB gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 654;
The probe set for capturing the IL1B gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 655;
the probe set for capturing IL4R gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 656;
the probe set for capturing XPO5 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 657;
the probe set for capturing GSTM1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 658;
the probe set for capturing KCNJ10 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 659.
The preparation method of the probe set for detecting the noise-related deafness gene in the embodiment is as follows:
PCR amplification system: 5. Mu.L of the probe set solution for detecting a noise-related deafness gene of the present example; 2. Mu.L of forward primer (25 uM) (the nucleotide sequence of the forward primer is AATGATACGGCGACCACCGA. Times.G, which represents that the intermediate has thio modification); 2. Mu.L of reverse primer (25. Mu.M) (the nucleotide sequence of the reverse primer is CAAGCAGAAGACGGCATACG. Times.A, & lt/EN & gt is thio-modified in the middle), mgCl 2 (50 mM), 4. Mu.L; 10x Platinum Taq polymerase buffer (from Life Technologies) 5. Mu.L; dNTPs (10 mM each) 4. Mu.L; platinum Taq polymerase (5U/. Mu.L, available from Life Technologies) 1. Mu.L; dH (dH) 2 O is added to 50 mu L;
amplification conditions: 98 ℃ for 30s; (98 ℃, 30s,60 ℃, 25s,72 ℃, 45 s) 35 cycles; 72 ℃ for 5min;
after purification of the PCR product using MinElute PCR purification kit (purchased from Life Technologies), 500ng was taken and the PCR product was bound using MyOne streptavidin magnetic beads (purchased from Invitrogen). Then alkaline NaOH is added to denature and elute the complementary strand without biotin; and washing the whole magnetic bead with formamide liquid at 100 ℃ to separate the probe from the magnetic bead, and precipitating with ethanol to obtain the biotin-labeled probe set.
Example 2
This example is a kit comprising the probe set for detecting a noise-related deafness gene of example 1, comprising:
(1) A probe set for detecting a noise-related deafness gene;
(2) An enrichment buffer, wherein the enrichment buffer contains a primer, human cot-1DNA and salmon DNA;
(3) Hybridization buffer containing SSPE buffer, denhardt solution, EDTA and SDS;
The hybridization buffer solution is composed of the following raw materials in parts by weight: 5-fold concentration of SSPE buffer, 5-fold concentration of Denhardt solution, 5mM EDTA, 0.1% SDS;
(4) A binding buffer solution, wherein the binding buffer solution contains NaCl, tris-HCl and EDTA;
the 1-fold concentration of binding buffer consisted of the following raw materials: 1M NaCl, 10mM Tris-HCl (pH 7.5), 1mM EDTA;
(5) A rinse solution 1, wherein the rinse solution 1 contains SSC and SDS;
the rinse liquid 1 is composed of the following raw materials in parts by weight: SSS, 0.1% sds at 1-fold concentration;
(6) A rinse solution 2, wherein the rinse solution 2 contains SSC and SDS;
the rinse liquid 1 is composed of the following raw materials in parts by weight: SSS, 0.1% sds at 1-fold concentration;
the SSS preparation method with 1-time concentration comprises the following steps: 175g of NaCl, 88g of trisodium citrate, adjusting the pH to 7.4 and dH 2 O constant volume to 1 liter;
(7) NaOH solution; the concentration of the NaOH solution is 0.1M;
(8) Tris-HCl buffer, pH 7.5;
(9) A PCR reaction solution containing dNTPs, a primer (the nucleotide sequence of the forward primer P5 is AATGATACGGCGACCACCGA G, the nucleotide sequence of the reverse primer P7 is CAAGCAGAAGACGGCATACG A, and the middle is thio-modified), a Phusion buffer solution, a hotspot Phusion enzyme, DMSO and dH 2 O；
The PCR reaction solution consists of the following raw materials in percentage by weight: mu.L dNTPs (1 each0 mM), 0.5. Mu.L of primer, 20. Mu.L of 5-fold concentration of Phusion buffer, 1. Mu. L Hotstart Phusion enzyme, 5. Mu.L of DMSO, 51. Mu.L of dH 2 O；
(10) TE buffer;
the TE buffer solution is prepared from the following raw materials in parts by weight: 10mM Tris-HCl,1mM EDTA, pH8.0, water to 500ml.
Conventional reagents used: SSPE buffer at 20-fold concentration, available from ameresco company; denhardt's solution at 50-fold concentration, available from USB company; EDTA solution: 0.5M, pH8.0, available from Meditech; phusion buffer, 5-fold strength, available from New England Biolab; the hotspot Phusion enzyme was purchased from New England Biolabs.
The using method of the kit for detecting the probe group of the noise-related deafness gene comprises the following steps:
s1, DNA extraction and whole genome library preparation:
DNA in peripheral blood was extracted using a Qiagen DNA mini kit (250) (from Qiagen) kit, quantified using a spectrophotometer, sample quality was checked by agarose gel electrophoresis, the whole genomic DNA electrophoresis band should generally be no less than 20kb, the concentration of DNA qualified for quality inspection was adjusted to 75 ng/. Mu.L, and the total amount of 3-5. Mu.g of DNA was randomly broken and amplified, thereby creating a whole genomic library.
S2, specific capture and sequencing of target pathogenic/susceptibility gene fragments
S201, preparing the following mixed system: taking 1 mu g S1 of a constructed whole genome library, 13 mu L of enrichment buffer and 5 mu L of the probe solution group for detecting the noise-related deafness gene of example 1; placing on a PCR instrument: 95 ℃ for 7min, and then 65 ℃ for 2min to obtain a mixed system;
s202, adding 23 mu L of hybridization buffer preheated at 65 ℃ into the mixed system obtained in the S201, and then hybridizing for 22 hours at 65 ℃ on a PCR instrument to obtain an enriched system mixture;
s203, vortex shaking the MyOne C1 streptavidin magnetic beads (purchased from Invitrogen) to fully suspend the magnetic beads, centrifuging briefly, centrifuging the magnetic beads to the bottom of a tube, and taking 50 mu L of MyOne C1 streptavidin magnetic beads to a new centrifuge tube with the volume of 1.5 mL;
s204, vortex oscillating a 1.5ml centrifuge tube filled with 50 mu LMyOne C1 streptavidin magnetic beads for at least 5S to enable the magnetic beads to be fully suspended, placing the magnetic beads on a magnetic rack after short centrifugation to keep still for one minute (without rotating the centrifuge tube), and carefully sucking and discarding the supernatant;
s205, taking down the centrifuge tube, adding 50 mu L of 1-time concentration binding buffer solution, shaking the rotary nest for 5S, standing on a magnetic rack for one minute after short centrifugation, carefully sucking and discarding the supernatant, and repeating for three times;
S206, taking down the centrifuge tube, adding 100 mu L of 2-time concentration binding buffer solution, vibrating the rotary nest for 5S, and standing on a magnetic rack for one minute after short centrifugation;
s207, adding the enrichment system mixture obtained in the S202 into a centrifuge tube in the S206, vibrating a rotary nest for at least 5S (without centrifugation), and placing the mixture on a rotator to rotate at a speed of 60 revolutions per minute for 1 hour at room temperature;
s208, washing the MyOne C1 streptavidin magnetic beads in the step S207 at room temperature by using a rinse solution 1 for 15 minutes, and then washing the beads 3 times by using a rinse solution 2 at 65 ℃ for 15 minutes each time.
S209, eluting MyOne C1 streptavidin magnetic beads in S208 with 0.1M NaOH solution at room temperature for 10 minutes, then vortex-shaking the eluent for at least 5 seconds, standing on a magnetic rack for one minute after short centrifugation, and transferring the supernatant into a clean centrifuge tube containing 70 mu L Tris-HCl buffer (1M, pH 7.5) to obtain a DNA solution;
s210, purifying the DNA solution obtained in S209 by using Qiagen MinElute Column (purchased from Qiagen), wherein the purification steps are described in a Qiagen MinElute Kit experimental operation manual;
s211, adding the DNA purified in the S210 into a PCR reaction solution for PCR amplification to obtain a PCR product;
The PCR reaction solution consists of the following raw materials in percentage by weight: 2. Mu.L dNTPs (10 mM each), 0.5. Mu.L primer 1 (AATGATACGGCGACCACCGA. Mu.G, 50 pmol), 0.5. Mu.L primer 2 (CAAGCAGAAGACGGCATACG. Mu.A, 50 pmol), 20. Mu.L Phusion buffer at 5-fold concentration, 1. Mu. L Hotstart Phusion enzyme, 5. Mu.L DMSO, 51. Mu.L dH 2 O; * Representing a thio modification in the middle;
the PCR amplification procedure was 98℃for 30s (1 cycle); 98 ℃, 25s,65 ℃, 30s,72 ℃, 30s (15 cycles); 72 ℃ for 5min (1 cycle);
s212, purifying the PCR product obtained in S211 according to a manual by using a Agencourt AMPure XP nucleic acid purification kit (purchased from Beckman Coulter) to obtain a DNA product;
s213, sequencing the DNA product obtained in the step S212 on an Illumina Nexseq 500 sequencer.
Example 3
The embodiment is the verification of the use effect of the probe set and the kit:
the detection of 10 samples by using the kit provided by the invention proves that the capture rate of the susceptibility gene is satisfactory, more than 97.7% of original short sequences can be compared with the reference sequence of the target region, the average effective sequencing data volume of the target region reaches 520.98Mb, and the average sequencing depth of the target region is 271X (see Table 1) which is far higher than the general genetic disease diagnosis requirement (generally 20X).
TABLE 1 quality of susceptibility gene sequencing data
Example 4
This example is a clinical diagnostic example of a probe set and kit of the invention:
10 patients with sensorineural hearing loss with a history of noise exposure were collected and screened for genes using the kit of the invention. The results are summarized in Table 2.
TABLE 2 Gene screening for sensorineural deafness patients with a history of noise exposure leading to hearing loss
Note that: "-" indicates that no significant mutation site was detected using the present invention.
In summary, the probe set and the kit for detecting the susceptibility genes of noise deafness provided by the invention have the characteristics of simple operation, low cost, good specificity, high sensitivity and the like, and can detect and definitely detect various types of mutations of 52 susceptibility genes at the same time, so that the probe set and the kit provided by the invention can be applied to screening of practitioners in related industries, and can provide occupational protection advice for high-risk groups. The invention can discover noise deafness sensitive individuals as soon as possible, provides basis for patients to take correct and reasonable intervention and treatment measures, and accords with the development trend of accurate medical treatment.
The above description is only of the preferred embodiments of the present invention, and is not intended to limit the present invention. Any simple modification, variation and equivalent variation of the above embodiments according to the technical substance of the invention still fall within the scope of the technical solution of the invention.
Sequence listing
<110> Chinese people's air force medical university
<120> A probe set for detecting noise-related deafness gene and kit therefor
<130> 2021.05.15
<160> 659
<170> SIPOSequenceListing 1.0
<210> 1
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 1
cctcctccgg ggcatggctt ccccttagcc ccctcctcca ccccaagtaa aaccataagc 60
tcccctcacc gcccttgtgc ccagatcgca gttgactgtt ccaaactcga gtgtctgatc 120
<210> 2
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 2
cttagccccc tcctccaccc caagtaaaac cataagctcc cctcaccgcc cttgtgccca 60
gatcgcagtt gactgttcca aactcgagtg tctgatccaa ccaagcccac cttacacagt 120
<210> 3
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 3
gtaaaaccat aagctcccct caccgccctt gtgcccagat cgcagttgac tgttccaaac 60
tcgagtgtct gatccaacca agcccacctt acacagtctt ccattccctc ttccctccct 120
<210> 4
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 4
cgcccttgtg cccagatcgc agttgactgt tccaaactcg agtgtctgat ccaaccaagc 60
ccaccttaca cagtcttcca ttccctcttc cctccctccc accctaacac ctcccacaca 120
<210> 5
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 5
tgactgttcc aaactcgagt gtctgatcca accaagccca ccttacacag tcttccattc 60
cctcttccct ccctcccacc ctaacacctc ccacacagaa aaaaaaaaaa acggcagaaa 120
<210> 6
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 6
tgatccaacc aagcccacct tacacagtct tccattccct cttccctccc tcccacccta 60
acacctccca cacagaaaaa aaaaaaaacg gcagaaatct agtcctgtaa actcccatcg 120
<210> 7
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 7
acagtcttcc attccctctt ccctccctcc caccctaaca cctcccacac agaaaaaaaa 60
aaaaacggca gaaatctagt cctgtaaact cccatcgttc tgaacggttc attttcacat 120
<210> 8
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 8
tccctcccac cctaacacct cccacacaga aaaaaaaaaa aacggcagaa atctagtcct 60
gtaaactccc atcgttctga acggttcatt ttcacatttc aaaaataatc gtttctgttt 120
<210> 9
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 9
acacagaaaa aaaaaaaaac ggcagaaatc tagtcctgta aactcccatc gttctgaacg 60
gttcattttc acatttcaaa aataatcgtt tctgtttact gagcatttgc taagtcccag 120
<210> 10
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 10
atgaactggg catttctgca gggcctgctg agtggcgtga acaagtactc cacagtgctg 60
agccgcatct ggctgtctgt ggtgttcatc tttcgtgtgc tggtgtacgt ggtggcagcg 120
<210> 11
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 11
cctgctgagt ggcgtgaaca agtactccac agtgctgagc cgcatctggc tgtctgtggt 60
gttcatcttt cgtgtgctgg tgtacgtggt ggcagcggag gaggtgtggg acgatgagca 120
<210> 12
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 12
actccacagt gctgagccgc atctggctgt ctgtggtgtt catctttcgt gtgctggtgt 60
acgtggtggc agcggaggag gtgtgggacg atgagcagaa ggactttgtc tgcaacacca 120
<210> 13
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 13
tggctgtctg tggtgttcat ctttcgtgtg ctggtgtacg tggtggcagc ggaggaggtg 60
tgggacgatg agcagaagga ctttgtctgc aacaccaagc agcccggctg ccccaacgtc 120
<210> 14
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 14
tcgtgtgctg gtgtacgtgg tggcagcgga ggaggtgtgg gacgatgagc agaaggactt 60
tgtctgcaac accaagcagc ccggctgccc caacgtctgc tatgacgagt tcttccccgt 120
<210> 15
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 15
cagcggagga ggtgtgggac gatgagcaga aggactttgt ctgcaacacc aagcagcccg 60
gctgccccaa cgtctgctat gacgagttct tccccgtgtc ccacgtgcgc ctctgggccc 120
<210> 16
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 16
gagcagaagg actttgtctg caacaccaag cagcccggct gccccaacgt ctgctatgac 60
gagttcttcc ccgtgtccca cgtgcgcctc tgggccctac agctcatcct ggtcacgtgc 120
<210> 17
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 17
caccaagcag cccggctgcc ccaacgtctg ctatgacgag ttcttccccg tgtcccacgt 60
gcgcctctgg gccctacagc tcatcctggt cacgtgcccc tcactgctcg tggtcatgca 120
<210> 18
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 18
acgtctgcta tgacgagttc ttccccgtgt cccacgtgcg cctctgggcc ctacagctca 60
tcctggtcac gtgcccctca ctgctcgtgg tcatgcacgt ggcctaccgc gaggaacgcg 120
<210> 19
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 19
cccgtgtccc acgtgcgcct ctgggcccta cagctcatcc tggtcacgtg cccctcactg 60
ctcgtggtca tgcacgtggc ctaccgcgag gaacgcgagc gcaagcacca cctgaaacac 120
<210> 20
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 20
ggccctacag ctcatcctgg tcacgtgccc ctcactgctc gtggtcatgc acgtggccta 60
ccgcgaggaa cgcgagcgca agcaccacct gaaacacggg cccaatgccc cgtccctgta 120
<210> 21
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 21
cgtgcccctc actgctcgtg gtcatgcacg tggcctaccg cgaggaacgc gagcgcaagc 60
accacctgaa acacgggccc aatgccccgt ccctgtacga caacctgagc aagaagcggg 120
<210> 22
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 22
atgcacgtgg cctaccgcga ggaacgcgag cgcaagcacc acctgaaaca cgggcccaat 60
gccccgtccc tgtacgacaa cctgagcaag aagcggggcg gactgtggtg gacgtacttg 120
<210> 23
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 23
acgcgagcgc aagcaccacc tgaaacacgg gcccaatgcc ccgtccctgt acgacaacct 60
gagcaagaag cggggcggac tgtggtggac gtacttgctg agcctcatct tcaaggccgc 120
<210> 24
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 24
aacacgggcc caatgccccg tccctgtacg acaacctgag caagaagcgg ggcggactgt 60
ggtggacgta cttgctgagc ctcatcttca aggccgccgt ggatgctggc ttcctctata 120
<210> 25
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 25
ctgtacgaca acctgagcaa gaagcggggc ggactgtggt ggacgtactt gctgagcctc 60
atcttcaagg ccgccgtgga tgctggcttc ctctatatct tccaccgcct ctacaaggat 120
<210> 26
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 26
gcggggcgga ctgtggtgga cgtacttgct gagcctcatc ttcaaggccg ccgtggatgc 60
tggcttcctc tatatcttcc accgcctcta caaggattat gacatgcccc gcgtggtggc 120
<210> 27
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 27
acttgctgag cctcatcttc aaggccgccg tggatgctgg cttcctctat atcttccacc 60
gcctctacaa ggattatgac atgccccgcg tggtggcctg ctccgtggag ccttgccccc 120
<210> 28
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 28
gccgccgtgg atgctggctt cctctatatc ttccaccgcc tctacaagga ttatgacatg 60
ccccgcgtgg tggcctgctc cgtggagcct tgcccccaca ctgtggactg ttacatctcc 120
<210> 29
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 29
ctatatcttc caccgcctct acaaggatta tgacatgccc cgcgtggtgg cctgctccgt 60
ggagccttgc ccccacactg tggactgtta catctcccgg cccacggaga agaaggtctt 120
<210> 30
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 30
aggattatga catgccccgc gtggtggcct gctccgtgga gccttgcccc cacactgtgg 60
actgttacat ctcccggccc acggagaaga aggtcttcac ctacttcatg gtgaccacag 120
<210> 31
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 31
gtggcctgct ccgtggagcc ttgcccccac actgtggact gttacatctc ccggcccacg 60
gagaagaagg tcttcaccta cttcatggtg accacagctg ccatctgcat cctgctcaac 120
<210> 32
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 32
cccccacact gtggactgtt acatctcccg gcccacggag aagaaggtct tcacctactt 60
catggtgacc acagctgcca tctgcatcct gctcaacctc agtgaagtct tctacctggt 120
<210> 33
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 33
tctcccggcc cacggagaag aaggtcttca cctacttcat ggtgaccaca gctgccatct 60
gcatcctgct caacctcagt gaagtcttct acctggtggg caagaggtgc atggagatct 120
<210> 34
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 34
gtcttcacct acttcatggt gaccacagct gccatctgca tcctgctcaa cctcagtgaa 60
gtcttctacc tggtgggcaa gaggtgcatg gagatcttcg gccccaggca ccggcggcct 120
<210> 35
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 35
cacagctgcc atctgcatcc tgctcaacct cagtgaagtc ttctacctgg tgggcaagag 60
gtgcatggag atcttcggcc ccaggcaccg gcggcctcgg tgccgggaat gcctacccga 120
<210> 36
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 36
tcaacctcag tgaagtcttc tacctggtgg gcaagaggtg catggagatc ttcggcccca 60
ggcaccggcg gcctcggtgc cgggaatgcc tacccgatac gtgcccacca tatgtcctct 120
<210> 37
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 37
ctggtgggca agaggtgcat ggagatcttc ggccccaggc accggcggcc tcggtgccgg 60
gaatgcctac ccgatacgtg cccaccatat gtcctctccc agggagggca ccctgaggat 120
<210> 38
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 38
gatcttcggc cccaggcacc ggcggcctcg gtgccgggaa tgcctacccg atacgtgccc 60
accatatgtc ctctcccagg gagggcaccc tgaggatggg aactctgtcc taatgaaggc 120
<210> 39
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 39
ggcctcggtg ccgggaatgc ctacccgata cgtgcccacc atatgtcctc tcccagggag 60
ggcaccctga ggatgggaac tctgtcctaa tgaaggctgg gtcggcccca gtggatgcag 120
<210> 40
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 40
cccgatacgt gcccaccata tgtcctctcc cagggagggc accctgagga tgggaactct 60
gtcctaatga aggctgggtc ggccccagtg gatgcaggtg ggtatccata acctgcgaga 120
<210> 41
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 41
cctctcccag ggagggcacc ctgaggatgg gaactctgtc ctaatgaagg ctgggtcggc 60
cccagtggat gcaggtgggt atccataacc tgcgagatca gcagataaga tcaacaggtc 120
<210> 42
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 42
aggatgggaa ctctgtccta atgaaggctg ggtcggcccc agtggatgca ggtgggtatc 60
cataacctgc gagatcagca gataagatca acaggtcccc cccacatgag gccacccagg 120
<210> 43
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 43
aaggctgggt cggccccagt ggatgcaggt gggtatccat aacctgcgag atcagcagat 60
aagatcaaca ggtccccccc acatgaggcc acccaggaaa aaaggcaggg gcagtggcat 120
<210> 44
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 44
tgcaggtggg tatccataac ctgcgagatc agcagataag atcaacaggt cccccccaca 60
tgaggccacc caggaaaaaa ggcaggggca gtggcatcct tgccgtagca gggtggtgag 120
<210> 45
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 45
caaccctgtg cagggcccag gagggctaaa ggagagggac tgccccctac agccagagag 60
atggaggtcg gggactaaga aaaactttcc agctggagag ggaatgccct gggacaaagt 120
<210> 46
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 46
ggctaaagga gagggactgc cccctacagc cagagagatg gaggtcgggg actaagaaaa 60
actttccagc tggagaggga atgccctggg acaaagtatc cccttttctg gctgggattc 120
<210> 47
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 47
gcccgtcccc tctgagcccc acacagaacc tgcatctttc tggctccaaa aggtgggaga 60
ttgcctagag tacaagccca gatttggcct gtcctgcggc agctgcaagc tctgggaaga 120
<210> 48
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 48
cagaacctgc atctttctgg ctccaaaagg tgggagattg cctagagtac aagcccagat 60
ttggcctgtc ctgcggcagc tgcaagctct gggaagagag gactgctgac tcctgtcttc 120
<210> 49
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 49
attctagaaa gtaatataaa gtctccatca caactagatt gtcattatac gtgagaattt 60
gagggacacc ctcccaacgg taatgagaca tgggcttttc ctttaggaac tttaatttct 120
<210> 50
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 50
tccatcacaa ctagattgtc attatacgtg agaatttgag ggacaccctc ccaacggtaa 60
tgagacatgg gcttttcctt taggaacttt aatttctgtg ccttcgggtg ggaatttttt 120
<210> 51
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 51
tttaaaacta aacacatagt gtttttaatt gatcttccaa taaaatatat ttttaatctg 60
ggaagatctc atttatctct ttttgaaaac aacaatagct caaagatttt aaactttttt 120
<210> 52
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 52
tttaattgat cttccaataa aatatatttt taatctggga agatctcatt tatctctttt 60
tgaaaacaac aatagctcaa agattttaaa cttttttcac ctaaaagcaa aatgaaaatg 120
<210> 53
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 53
atatttttaa tctgggaaga tctcatttat ctctttttga aaacaacaat agctcaaaga 60
ttttaaactt ttttcaccta aaagcaaaat gaaaatgtcc tcaatgttca tttatttgtt 120
<210> 54
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 54
catttatctc tttttgaaaa caacaatagc tcaaagattt taaacttttt tcacctaaaa 60
gcaaaatgaa aatgtcctca atgttcattt atttgtttgt ttgttttggc tttccattgt 120
<210> 55
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 55
caatagctca aagattttaa acttttttca cctaaaagca aaatgaaaat gtcctcaatg 60
ttcatttatt tgtttgtttg ttttggcttt ccattgtctc caaaagtttg aatactaaaa 120
<210> 56
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 56
tttttcacct aaaagcaaaa tgaaaatgtc ctcaatgttc atttatttgt ttgtttgttt 60
tggctttcca ttgtctccaa aagtttgaat actaaaaaag taaaaaagat aaaaagtaga 120
<210> 57
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 57
aaatgtcctc aatgttcatt tatttgtttg tttgttttgg ctttccattg tctccaaaag 60
tttgaatact aaaaaagtaa aaaagataaa aagtagaaag aatattgatt ctgacaaaat 120
<210> 58
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 58
ttgtttgttt gttttggctt tccattgtct ccaaaagttt gaatactaaa aaagtaaaaa 60
agataaaaag tagaaagaat attgattctg acaaaataca ccacctaaat gtagcagccc 120
<210> 59
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 59
attgtctcca aaagtttgaa tactaaaaaa gtaaaaaaga taaaaagtag aaagaatatt 60
gattctgaca aaatacacca cctaaatgta gcagccctgc ctcattcttt agtttttaat 120
<210> 60
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 60
atattgtgaa ccatttaaaa cgtttccaga taatcatatc tacttgttag aaaaggtaat 60
ttaaactact caacacagat aatttcactt caggatcagc tgtttcatga attttctagg 120
<210> 61
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 61
ttccagataa tcatatctac ttgttagaaa aggtaattta aactactcaa cacagataat 60
ttcacttcag gatcagctgt ttcatgaatt ttctagggag ttaatattct cttttcttta 120
<210> 62
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 62
ttagaaaagg taatttaaac tactcaacac agataatttc acttcaggat cagctgtttc 60
atgaattttc tagggagtta atattctctt ttctttattt ttttctctgt cacccaggct 120
<210> 63
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 63
tcaacacaga taatttcact tcaggatcag ctgtttcatg aattttctag ggagttaata 60
ttctcttttc tttatttttt tctctgtcac ccaggctgga gtgccatggc ataaactcgg 120
<210> 64
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 64
gatttattag actgcaagga taaactaagt ccaggaaact cagtcagagc tctgttctgt 60
atatgcacat tcttcctcag tatcataaag ggccttcctt tgtacttcct atgactggga 120
<210> 65
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 65
actaagtcca ggaaactcag tcagagctct gttctgtata tgcacattct tcctcagtat 60
cataaagggc cttcctttgt acttcctatg actgggagcc taactttaat atactgaagg 120
<210> 66
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 66
agctccaaat caactaattc ttggtagtta agtatagaaa caccataggg actttgcagg 60
atcagttgag tctcccattg ttctcactag acaactgata cttattagca gtttattgag 120
<210> 67
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 67
gtagttaagt atagaaacac catagggact ttgcaggatc agttgagtct cccattgttc 60
tcactagaca actgatactt attagcagtt tattgagtat acttcactaa catggttttt 120
<210> 68
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 68
aagcaaatgg aagttttcac tttctactat agtcataaac ttttgctgat aggtcattta 60
taagaaggaa tgaataaaag taggtctcta ccatttccat aaagatagac taacagagaa 120
<210> 69
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 69
ctactatagt cataaacttt tgctgatagg tcatttataa gaaggaatga ataaaagtag 60
gtctctacca tttccataaa gatagactaa cagagaacat gcagtatcag ttgattatca 120
<210> 70
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 70
ttgattgcaa aagagcaaat tgcaaatatc caaagtaatc cttgtaatac acgtgacatt 60
ataaatttta cgatgcagtg cctatgattc acgcacactg tacctgaacc agctgtgaaa 120
<210> 71
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 71
aaatatccaa agtaatcctt gtaatacacg tgacattata aattttacga tgcagtgcct 60
atgattcacg cacactgtac ctgaaccagc tgtgaaacat tcatgaatca taaactcctg 120
<210> 72
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 72
attctggtta gagatggact ctggacgcta gataaaacac ttgggattaa aacacttatc 60
ctagaactta tcattatagg cacttcacat aatgggggaa actgaatatt tacaataacc 120
<210> 73
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 73
gacgctagat aaaacacttg ggattaaaac acttatccta gaacttatca ttataggcac 60
ttcacataat gggggaaact gaatatttac aataaccttt tggaatagtt gatgatgatg 120
<210> 74
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 74
ttaaaacact tatcctagaa cttatcatta taggcacttc acataatggg ggaaactgaa 60
tatttacaat aaccttttgg aatagttgat gatgatgatg atgggaaaga gagagagaaa 120
<210> 75
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 75
atcattatag gcacttcaca taatggggga aactgaatat ttacaataac cttttggaat 60
agttgatgat gatgatgatg ggaaagagag agagaaaggc caggtgtggt ggctcatgcc 120
<210> 76
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 76
tgggggaaac tgaatattta caataacctt ttggaatagt tgatgatgat gatgatggga 60
aagagagaga gaaaggccag gtgtggtggc tcatgcctgt aatctcagca ctttgggagg 120
<210> 77
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 77
atgcattagc atgtccttca ttatttgttt taatcaagaa gaactgaaat aaggaatatg 60
taacataaat ttagttttgc taacgaatac atcagagaga aatatatctc cttagagagg 120
<210> 78
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 78
tttgttttaa tcaagaagaa ctgaaataag gaatatgtaa cataaattta gttttgctaa 60
cgaatacatc agagagaaat atatctcctt agagaggaat atattttatc taggtatttt 120
<210> 79
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 79
aaataaggaa tatgtaacat aaatttagtt ttgctaacga atacatcaga gagaaatata 60
tctccttaga gaggaatata ttttatctag gtattttttt tgaacagtga agcacttttt 120
<210> 80
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 80
ctgttacaag agtcataaaa tatgcaggtc atttatcata catggaaaag ttgtggggct 60
tttttctttc tattttaaaa tttattatgt gtcttcattg aaccttgtga ggcaaaaatt 120
<210> 81
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 81
gcaggtcatt tatcatacat ggaaaagttg tggggctttt ttctttctat tttaaaattt 60
attatgtgtc ttcattgaac cttgtgaggc aaaaattcta atactaccat tttcgttttc 120
<210> 82
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 82
aggctggtat gctaagttaa ataagccact catgaaaaaa caaatatggc atgattatac 60
ttgaaactga cccaatactt ccatatattt tttaataaac atagatgtgt actcttctga 120
<210> 83
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 83
agccactcat gaaaaaacaa atatggcatg attatacttg aaactgaccc aatacttcca 60
tatatttttt aataaacata gatgtgtact cttctgatct gaaagtttga aatttaccaa 120
<210> 84
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 84
tggcatgatt atacttgaaa ctgacccaat acttccatat attttttaat aaacatagat 60
gtgtactctt ctgatctgaa agtttgaaat ttaccaaatg atcttcaggc ctctcaaagt 120
<210> 85
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 85
acccaatact tccatatatt ttttaataaa catagatgtg tactcttctg atctgaaagt 60
ttgaaattta ccaaatgatc ttcaggcctc tcaaagtatc aaaaaactca ccatatcacc 120
<210> 86
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 86
taataaacat agatgtgtac tcttctgatc tgaaagtttg aaatttacca aatgatcttc 60
aggcctctca aagtatcaaa aaactcacca tatcaccaca tccagacaat gagatgcagg 120
<210> 87
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 87
tctgatctga aagtttgaaa tttaccaaat gatcttcagg cctctcaaag tatcaaaaaa 60
ctcaccatat caccacatcc agacaatgag atgcaggatc cctcattcat tatgcttgct 120
<210> 88
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 88
tgtagatatt tctgagtgat ttcacagaat gtgtgggaat atacatctgc gtcaattctt 60
taaatgagta aaaggagcat ctgtaatgca gcaaaatcaa tcatgaagaa gtaactgatg 120
<210> 89
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 89
acagaatgtg tgggaatata catctgcgtc aattctttaa atgagtaaaa ggagcatctg 60
taatgcagca aaatcaatca tgaagaagta actgatgcag gaattgccta tacaggtttc 120
<210> 90
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 90
ctgcgtcaat tctttaaatg agtaaaagga gcatctgtaa tgcagcaaaa tcaatcatga 60
agaagtaact gatgcaggaa ttgcctatac aggtttccaa agtacatgta cgcgtgtaga 120
<210> 91
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 91
aaaaggagca tctgtaatgc agcaaaatca atcatgaaga agtaactgat gcaggaattg 60
cctatacagg tttccaaagt acatgtacgc gtgtagattg agttacaaga aaaaggtcac 120
<210> 92
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 92
aaaatcaatc atgaagaagt aactgatgca ggaattgcct atacaggttt ccaaagtaca 60
tgtacgcgtg tagattgagt tacaagaaaa aggtcacttt ctaataaggc aactgacaat 120
<210> 93
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 93
tgatgcagga attgcctata caggtttcca aagtacatgt acgcgtgtag attgagttac 60
aagaaaaagg tcactttcta ataaggcaac tgacaattat tttgtaaaaa atatgaaagt 120
<210> 94
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 94
gtttccaaag tacatgtacg cgtgtagatt gagttacaag aaaaaggtca ctttctaata 60
aggcaactga caattatttt gtaaaaaata tgaaagtgca agcacatact ttagaacaaa 120
<210> 95
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 95
taactttctc tgtggttcac tgctgcctta aatttgaaaa aaataagaga agggcggggg 60
aagaacttac ttgcggttta aagtttcatc tttagtccat gtaagattat gcagcaagtt 120
<210> 96
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 96
tgccttaaat ttgaaaaaaa taagagaagg gcgggggaag aacttacttg cggtttaaag 60
tttcatcttt agtccatgta agattatgca gcaagttttc ttgccaaggc tgcctcttgc 120
<210> 97
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 97
atgtgaaaat gcaatattta cttcgggatg aataaaggtt aagttttaac cagtttgaaa 60
acacagtaag caaaatggag tcactgtaat atttactact agagatgata ttttctttga 120
<210> 98
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 98
cgggatgaat aaaggttaag ttttaaccag tttgaaaaca cagtaagcaa aatggagtca 60
ctgtaatatt tactactaga gatgatattt tctttgaaaa aagtgttgca taatattctg 120
<210> 99
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 99
tcctctccat acctctccgg ctccttctgt ctgtctccat atcctttgac tgaccgactc 60
ccattgacag aatttggtaa gtgagcctct gaagggcagg ggttgggctt ggggagggct 120
<210> 100
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 100
cttctgtctg tctccatatc ctttgactga ccgactccca ttgacagaat ttggtaagtg 60
agcctctgaa gggcaggggt tgggcttggg gagggcttgt ggtgggcaca gtggggaggg 120
<210> 101
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 101
gcagcgcggt ctcgacggtg gggaggacaa ggcgcccatt ctccaagtta ctagggtggc 60
gcaagcgcct ctccctttaa ctgcccaggc agccccgcct gctatgcccg gcagtcaaac 120
<210> 102
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 102
aggacaaggc gcccattctc caagttacta gggtggcgca agcgcctctc cctttaactg 60
cccaggcagc cccgcctgct atgcccggca gtcaaaccag gtccagaccc cggctgtgcc 120
<210> 103
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 103
ctgaacctat taaaaaagtt gaataaaatg gaaacttttg gcatgggaga aaaaaaaaaa 60
aaacagatgt aagttagaag aggttacgca aaaatcctcc atccctgcat ttgaatgagg 120
<210> 104
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 104
taaaatggaa acttttggca tgggagaaaa aaaaaaaaaa cagatgtaag ttagaagagg 60
ttacgcaaaa atcctccatc cctgcatttg aatgaggact atcagtatgc atccaggatg 120
<210> 105
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 105
gagaaaaaaa aaaaaaacag atgtaagtta gaagaggtta cgcaaaaatc ctccatccct 60
gcatttgaat gaggactatc agtatgcatc caggatgacg tttacttaaa tatctctgtt 120
<210> 106
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 106
taagttagaa gaggttacgc aaaaatcctc catccctgca tttgaatgag gactatcagt 60
atgcatccag gatgacgttt acttaaatat ctctgtttcc tggaaaggcc caggaccaag 120
<210> 107
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 107
aatcctccat ccctgcattt gaatgaggac tatcagtatg catccaggat gacgtttact 60
taaatatctc tgtttcctgg aaaggcccag gaccaaggac cactcagcag ctaagagcat 120
<210> 108
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 108
tgaggactat cagtatgcat ccaggatgac gtttacttaa atatctctgt ttcctggaaa 60
ggcccaggac caaggaccac tcagcagcta agagcatctc tagaccccag actgtggcct 120
<210> 109
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 109
ggatgacgtt tacttaaata tctctgtttc ctggaaaggc ccaggaccaa ggaccactca 60
gcagctaaga gcatctctag accccagact gtggcctcta ggtaccattt ccccctgaaa 120
<210> 110
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 110
ctgtttcctg gaaaggccca ggaccaagga ccactcagca gctaagagca tctctagacc 60
ccagactgtg gcctctaggt accatttccc cctgaaagaa accagggccc ctggcggatt 120
<210> 111
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 111
ccaaggacca ctcagcagct aagagcatct ctagacccca gactgtggcc tctaggtacc 60
atttccccct gaaagaaacc agggcccctg gcggattcca ggtctgtggc agaagatgtg 120
<210> 112
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 112
agcatctcta gaccccagac tgtggcctct aggtaccatt tccccctgaa agaaaccagg 60
gcccctggcg gattccaggt ctgtggcaga agatgtgcaa gctaagcctg gagtattcat 120
<210> 113
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 113
ggcctctagg taccatttcc ccctgaaaga aaccagggcc cctggcggat tccaggtctg 60
tggcagaaga tgtgcaagct aagcctggag tattcatcac agcagaaggc agggaagtta 120
<210> 114
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 114
tgaaagaaac cagggcccct ggcggattcc aggtctgtgg cagaagatgt gcaagctaag 60
cctggagtat tcatcacagc agaaggcagg gaagttatca gaggccccct agggccatgt 120
<210> 115
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 115
ggattccagg tctgtggcag aagatgtgca agctaagcct ggagtattca tcacagcaga 60
aggcagggaa gttatcagag gccccctagg gccatgtcac tggaacttga aggggtcctt 120
<210> 116
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 116
atgtgcaagc taagcctgga gtattcatca cagcagaagg cagggaagtt atcagaggcc 60
ccctagggcc atgtcactgg aacttgaagg ggtccttgtc ttcccccttc tccccaagct 120
<210> 117
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 117
ttcatcacag cagaaggcag ggaagttatc agaggccccc tagggccatg tcactggaac 60
ttgaaggggt ccttgtcttc ccccttctcc ccaagctgaa gatgcagtgg gtaagcctca 120
<210> 118
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 118
tcaagacatt ctaaaattaa gaacctatgc tggatatctc tgatttcaac gtatgtttaa 60
aatatatgtt attaattaca agtttaatta atgaaagtct aaagaagaaa agtctactta 120
<210> 119
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 119
cctatgctgg atatctctga tttcaacgta tgtttaaaat atatgttatt aattacaagt 60
ttaattaatg aaagtctaaa gaagaaaagt ctacttagaa gtaaaaacat cagcaatatt 120
<210> 120
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 120
caacgtatgt ttaaaatata tgttattaat tacaagttta attaatgaaa gtctaaagaa 60
gaaaagtcta cttagaagta aaaacatcag caatattact gtgtaataga tctgaacatt 120
<210> 121
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 121
tattaattac aagtttaatt aatgaaagtc taaagaagaa aagtctactt agaagtaaaa 60
acatcagcaa tattactgtg taatagatct gaacattaaa ggggcatata tgcatatctg 120
<210> 122
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 122
gaaagtctaa agaagaaaag tctacttaga agtaaaaaca tcagcaatat tactgtgtaa 60
tagatctgaa cattaaaggg gcatatatgc atatctgtac accgaggcaa atttatagct 120
<210> 123
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 123
acttagaagt aaaaacatca gcaatattac tgtgtaatag atctgaacat taaaggggca 60
tatatgcata tctgtacacc gaggcaaatt tatagctaca ggcattttcc ttttttgaaa 120
<210> 124
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 124
ttcaaatatt tagtccattt ggagtctact tttcctaggg gctcagttag gaatccatat 60
tcctattcct ccattttagg gctggctttc caacatcatc ttctgaacag tccccactga 120
<210> 125
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 125
tctacttttc ctaggggctc agttaggaat ccatattcct attcctccat tttagggctg 60
gctttccaac atcatcttct gaacagtccc cactgatatg tggtgccacc tttatcacat 120
<210> 126
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 126
aggaatccat attcctattc ctccatttta gggctggctt tccaacatca tcttctgaac 60
agtccccact gatatgtggt gccaccttta tcacatgtcc ccttcctcta gacttggagt 120
<210> 127
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 127
attttagggc tggctttcca acatcatctt ctgaacagtc cccactgata tgtggtgcca 60
cctttatcac atgtcccctt cctctagact tggagttacc tctgagctct cttctgggcc 120
<210> 128
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 128
catcttctga acagtcccca ctgatatgtg gtgccacctt tatcacatgt ccccttcctc 60
tagacttgga gttacctctg agctctcttc tgggcctctg tcaccacacc gctctgattg 120
<210> 129
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 129
tatgtggtgc cacctttatc acatgtcccc ttcctctaga cttggagtta cctctgagct 60
ctcttctggg cctctgtcac cacaccgctc tgattgctga agttggcgtg tgccctggta 120
<210> 130
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 130
gtccccttcc tctagacttg gagttacctc tgagctctct tctgggcctc tgtcaccaca 60
ccgctctgat tgctgaagtt ggcgtgtgcc ctggtatctg ccagcagaag tcctctgctc 120
<210> 131
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 131
tacctctgag ctctcttctg ggcctctgtc accacaccgc tctgattgct gaagttggcg 60
tgtgccctgg tatctgccag cagaagtcct ctgctctcct tttcaaaagt tcagttggca 120
<210> 132
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 132
tctgtcacca caccgctctg attgctgaag ttggcgtgtg ccctggtatc tgccagcaga 60
agtcctctgc tctccttttc aaaagttcag ttggcattta caaatatttt tcttccacat 120
<210> 133
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 133
ctgaagttgg cgtgtgccct ggtatctgcc agcagaagtc ctctgctctc cttttcaaaa 60
gttcagttgg catttacaaa tatttttctt ccacatacat tttagaatat gtttgttaaa 120
<210> 134
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 134
tctgccagca gaagtcctct gctctccttt tcaaaagttc agttggcatt tacaaatatt 60
tttcttccac atacatttta gaatatgttt gttaaattac tcaagccctc caactggaag 120
<210> 135
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 135
tccttttcaa aagttcagtt ggcatttaca aatatttttc ttccacatac attttagaat 60
atgtttgtta aattactcaa gccctccaac tggaagtttg agtggagtcc tgttgaactt 120
<210> 136
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 136
tttacaaata tttttcttcc acatacattt tagaatatgt ttgttaaatt actcaagccc 60
tccaactgga agtttgagtg gagtcctgtt gaacttaaca gatttggaaa atcctgacgt 120
<210> 137
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 137
acattttaga atatgtttgt taaattactc aagccctcca actggaagtt tgagtggagt 60
cctgttgaac ttaacagatt tggaaaatcc tgacgtcatt ctaatgccaa gttgccctgt 120
<210> 138
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 138
aagtccctta ctgtccgtgt ccctccatgc cagctcactg gaggggctcc tgctgaaaga 60
ggtggggttc agttcccaaa cccactggag ggttgcaagg agacgcttct agtatgttag 120
<210> 139
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 139
ccatgccagc tcactggagg ggctcctgct gaaagaggtg gggttcagtt cccaaaccca 60
ctggagggtt gcaaggagac gcttctagta tgttagacag cacagtcttc caaatggact 120
<210> 140
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 140
gtgtggctcg tcatgggcga tgctgtctct gtctcctggc tctgaggccg tggctgtcaa 60
tctcttcaac tgtgatttgt tcttttcccc ctttgcagac tgtccttgga cccgccagca 120
<210> 141
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 141
gtctctgtct cctggctctg aggccgtggc tgtcaatctc ttcaactgtg atttgttctt 60
ttcccccttt gcagactgtc cttggacccg ccagcatttg gctctggggc cctagagaga 120
<210> 142
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 142
cgtggctgtc aatctcttca actgtgattt gttcttttcc ccctttgcag actgtccttg 60
gacccgccag catttggctc tggggcccta gagagaccct gggatgtgga tcacactggt 120
<210> 143
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 143
tgatttgttc ttttccccct ttgcagactg tccttggacc cgccagcatt tggctctggg 60
gccctagaga gaccctggga tgtggatcac actggtgacc cctgtgagcc ccactgggga 120
<210> 144
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 144
agactgtcct tggacccgcc agcatttggc tctggggccc tagagagacc ctgggatgtg 60
gatcacactg gtgacccctg tgagccccac tggggacaga tccaggaagg gacctcctgg 120
<210> 145
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 145
tttggctctg gggccctaga gagaccctgg gatgtggatc acactggtga cccctgtgag 60
ccccactggg gacagatcca ggaagggacc tcctgggaat gaggtctggc ctctgcctgg 120
<210> 146
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 146
agtgttactg agttgaggtg actgggttga ttttagcttc acaggtgatc tgaacccagc 60
tgagtggctt ccatggagct gatctgaacc cagctgagtg gcttccatgg agctgatctg 120
<210> 147
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 147
ggttgatttt agcttcacag gtgatctgaa cccagctgag tggcttccat ggagctgatc 60
tgaacccagc tgagtggctt ccatggagct gatctgaacc cagttgagtg gcttccatga 120
<210> 148
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 148
tctgaaccca gctgagtggc ttccatggag ctgatctgaa cccagctgag tggcttccat 60
ggagctgatc tgaacccagt tgagtggctt ccatgaagct gatccggact aagctaaact 120
<210> 149
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 149
gagcttctca gtagagttgt acagagtgga aatgagtcgg caggctgcat ggggggttcc 60
agtcacagca cagagggtgt gtgttttcgg tgcctgccct cctgagctcc aagtgtccca 120
<210> 150
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 150
agtggaaatg agtcggcagg ctgcatgggg ggttccagtc acagcacaga gggtgtgtgt 60
tttcggtgcc tgccctcctg agctccaagt gtcccacgcc tgcccgtcag tcctcatgtc 120
<210> 151
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 151
tgtagaacac tcactcaaga tcttccttta ttcctgccca ctttgtcaca ggcagaacaa 60
cgtaggagtg gagatgtgat ctccagagcc acactgtctc gttcgaatcc tggccccatt 120
<210> 152
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 152
cctttattcc tgcccacttt gtcacaggca gaacaacgta ggagtggaga tgtgatctcc 60
agagccacac tgtctcgttc gaatcctggc cccattgcaa actatctggg taacttcggg 120
<210> 153
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 153
caggcagaac aacgtaggag tggagatgtg atctccagag ccacactgtc tcgttcgaat 60
cctggcccca ttgcaaacta tctgggtaac ttcgggaggt tacttaacct ttctgtacct 120
<210> 154
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 154
gatgtgatct ccagagccac actgtctcgt tcgaatcctg gccccattgc aaactatctg 60
ggtaacttcg ggaggttact taacctttct gtaccttagt gccctcagat gtaaagtgaa 120
<210> 155
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 155
tctcgttcga atcctggccc cattgcaaac tatctgggta acttcgggag gttacttaac 60
ctttctgtac cttagtgccc tcagatgtaa agtgaaggta gtaagagtcc attccctctt 120
<210> 156
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 156
gcaaactatc tgggtaactt cgggaggtta cttaaccttt ctgtacctta gtgccctcag 60
atgtaaagtg aaggtagtaa gagtccattc cctcttatag gattgttata aggattagat 120
<210> 157
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 157
aggttactta acctttctgt accttagtgc cctcagatgt aaagtgaagg tagtaagagt 60
ccattccctc ttataggatt gttataagga ttagatgggt taatattgga aaatccctca 120
<210> 158
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 158
tagtgccctc agatgtaaag tgaaggtagt aagagtccat tccctcttat aggattgtta 60
taaggattag atgggttaat attggaaaat ccctcagtgt agagcctaga catagtaaga 120
<210> 159
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 159
ggtagtaaga gtccattccc tcttatagga ttgttataag gattagatgg gttaatattg 60
gaaaatccct cagtgtagag cctagacata gtaagagcaa tattaagtgt ttgtcaatta 120
<210> 160
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 160
ataggattgt tataaggatt agatgggtta atattggaaa atccctcagt gtagagccta 60
gacatagtaa gagcaatatt aagtgtttgt caattaaaca aaatatgttt tgaaaggatg 120
<210> 161
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 161
gggttaatat tggaaaatcc ctcagtgtag agcctagaca tagtaagagc aatattaagt 60
gtttgtcaat taaacaaaat atgttttgaa aggatggttt tgtgggatag gaaggtttgg 120
<210> 162
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 162
gtgtagagcc tagacatagt aagagcaata ttaagtgttt gtcaattaaa caaaatatgt 60
tttgaaagga tggttttgtg ggataggaag gtttggttta gggcagtggt tggcctttgt 120
<210> 163
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 163
gcaatattaa gtgtttgtca attaaacaaa atatgttttg aaaggatggt tttgtgggat 60
aggaaggttt ggtttagggc agtggttggc ctttgttcat cataatgtga ccttggagcc 120
<210> 164
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 164
aacaaaatat gttttgaaag gatggttttg tgggatagga aggtttggtt tagggcagtg 60
gttggccttt gttcatcata atgtgacctt ggagccaaac ttcagtgtcc agaggcagtc 120
<210> 165
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 165
aaagcgggaa agatatggtg cagccaaata ggcagagcag aaggtatgtg ctgctactgc 60
atataacacc actgctactt tttaaagcaa atcccacctt cttctttggc ttgctcaagc 120
<210> 166
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 166
caaataggca gagcagaagg tatgtgctgc tactgcatat aacaccactg ctacttttta 60
aagcaaatcc caccttcttc tttggcttgc tcaagcctgt tccacagtga acaggagtca 120
<210> 167
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 167
agtaacaaaa acaaaggaaa aaaaaacatg ctttttttta tataatgaat aaaggttaag 60
aaatgactcc aggtctctta tttattttgt tgatatttgt gttggttagt taagaagatg 120
<210> 168
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 168
aacatgcttt tttttatata atgaataaag gttaagaaat gactccaggt ctcttattta 60
ttttgttgat atttgtgttg gttagttaag aagatgattt gctcgattat ttcaacattt 120
<210> 169
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 169
catagctttt taaagacaca aagcttttca aaattcctgc ttacctgggg gtaaaatttg 60
gggaagcaga tttctccagt gtttaaagaa gccaatttgg cagtgtacca gagttgaata 120
<210> 170
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 170
ttttcaaaat tcctgcttac ctgggggtaa aatttgggga agcagatttc tccagtgttt 60
aaagaagcca atttggcagt gtaccagagt tgaatacatt tttcccatca caagggaata 120
<210> 171
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 171
cggcctgaag gatgctgata accgggagcc ccgccctggg ttcggctatc ccgggcaccc 60
cgggccggcg gggcgaggct ctccaattgc tgggccagag cgggaccctt cctttccgca 120
<210> 172
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 172
ggagccccgc cctgggttcg gctatcccgg gcaccccggg ccggcggggc gaggctctcc 60
aattgctggg ccagagcggg acccttcctt tccgcaccct cctgggtatc tccggtcttc 120
<210> 173
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 173
ggcgggacga gggggtggtg ctgattggct gagcctgaag tcgccacgga ctcggggcaa 60
caggcagatt tgcctgctga gggtggagac ccacgagccg aggcctcctg cagtgttctg 120
<210> 174
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 174
ttggctgagc ctgaagtcgc cacggactcg gggcaacagg cagatttgcc tgctgagggt 60
ggagacccac gagccgaggc ctcctgcagt gttctgcaca gcaaaccgca cgctatggct 120
<210> 175
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 175
gactcggggc aacaggcaga tttgcctgct gagggtggag acccacgagc cgaggcctcc 60
tgcagtgttc tgcacagcaa accgcacgct atggctgaca gccgggatcc cgccagcgac 120
<210> 176
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 176
cctgctgagg gtggagaccc acgagccgag gcctcctgca gtgttctgca cagcaaaccg 60
cacgctatgg ctgacagccg ggatcccgcc agcgaccaga tgcagcactg gaaggagcag 120
<210> 177
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 177
ttccacttcc caattagttg tgatgcttta ctgatctagg agagctttat ggtttgggac 60
tactataggc gaacactctg ttgcctcctc tctcaagaat cagagtgtcc tattttggaa 120
<210> 178
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 178
gctttactga tctaggagag ctttatggtt tgggactact ataggcgaac actctgttgc 60
ctcctctctc aagaatcaga gtgtcctatt ttggaaacac agtcaaccat gtgaaagcaa 120
<210> 179
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 179
ttgtatatac atgagcttgt ggggaaagct gttatataaa tgaggacaat tgatctaact 60
gttagagtgg ggaaatggaa gagattctga agcctccatt tgtaacttaa aaactcaggt 120
<210> 180
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 180
aaagctgtta tataaatgag gacaattgat ctaactgtta gagtggggaa atggaagaga 60
ttctgaagcc tccatttgta acttaaaaac tcaggtttca tttcttaaca ggattgatgt 120
<210> 181
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 181
cacgaagttt catgaacgag gtagcatttg agattgtttt gaagaataga taggattttg 60
atttttagta ggtaaaatgc ggcaggagaa ggacatgagt ataggcacaa aggtgggaca 120
<210> 182
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 182
catttgagat tgttttgaag aatagatagg attttgattt ttagtaggta aaatgcggca 60
ggagaaggac atgagtatag gcacaaaggt gggacatgtt aggtgtgttg aaactataaa 120
<210> 183
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 183
gataggattt tgatttttag taggtaaaat gcggcaggag aaggacatga gtataggcac 60
aaaggtggga catgttaggt gtgttgaaac tataaacagt cactctctcc tgaatgagaa 120
<210> 184
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 184
taaaatgcgg caggagaagg acatgagtat aggcacaaag gtgggacatg ttaggtgtgt 60
tgaaactata aacagtcact ctctcctgaa tgagaagaat agagtttggg taggttcatt 120
<210> 185
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 185
gagtataggc acaaaggtgg gacatgttag gtgtgttgaa actataaaca gtcactctct 60
cctgaatgag aagaatagag tttgggtagg ttcattgagt atatgtcaga ggggacctta 120
<210> 186
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 186
tgttaggtgt gttgaaacta taaacagtca ctctctcctg aatgagaaga atagagtttg 60
ggtaggttca ttgagtatat gtcagagggg accttaaatg cttgagtagg agtgaaaagc 120
<210> 187
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 187
taatctgtac gtaagaacag agatggtatt ttctttcttt cgactccata tgtaactgta 60
aactgctacc agactcttaa tttgaacatc atcattttca gatgtttacc cttaaaaatg 120
<210> 188
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 188
ggtattttct ttctttcgac tccatatgta actgtaaact gctaccagac tcttaatttg 60
aacatcatca ttttcagatg tttaccctta aaaatggaaa tgccagtatc tcgaggactc 120
<210> 189
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 189
tatagccttt ggacagcctt ctgagacact gataaaatag caaatgcccc aactttgaaa 60
attacttaaa tctcttgtcc atttgctcac ttacctgtga ttgacttttt aaaacaaggg 120
<210> 190
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 190
gacactgata aaatagcaaa tgccccaact ttgaaaatta cttaaatctc ttgtccattt 60
gctcacttac ctgtgattga ctttttaaaa caagggctca gaaccactgg tcactttcct 120
<210> 191
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 191
tgttatcaac ttacacttaa acctttccca gacatcttga agactgaacc tcaccattct 60
cagctcaaaa cgtgtttttc tttgtgtgtg tgggcgtgtg agatggcctg gattcagagt 120
<210> 192
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 192
ttcccagaca tcttgaagac tgaacctcac cattctcagc tcaaaacgtg tttttctttg 60
tgtgtgtggg cgtgtgagat ggcctggatt cagagtgatc cctggggaat gctgccaaga 120
<210> 193
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 193
aagctgagat ggatgaactg acagaagtag gtatcagaaa gtgtgtaatg acaaactttg 60
ctgagctaaa ggattatatt ctaacccaag gcaaagaagc taagaaccgt gataaaagat 120
<210> 194
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 194
aagtaggtat cagaaagtgt gtaatgacaa actttgctga gctaaaggat tatattctaa 60
cccaaggcaa agaagctaag aaccgtgata aaagattaca ggaactgtta attagaataa 120
<210> 195
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 195
tgacaaactt tgctgagcta aaggattata ttctaaccca aggcaaagaa gctaagaacc 60
gtgataaaag attacaggaa ctgttaatta gaataaccag tttagagagg aatacaaatg 120
<210> 196
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 196
attatattct aacccaaggc aaagaagcta agaaccgtga taaaagatta caggaactgt 60
taattagaat aaccagttta gagaggaata caaatgacat gatggagctg agaaacacag 120
<210> 197
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 197
aagctaagaa ccgtgataaa agattacagg aactgttaat tagaataacc agtttagaga 60
ggaatacaaa tgacatgatg gagctgagaa acacagcgtg agagcttcat gattcaaaca 120
<210> 198
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 198
tacaggaact gttaattaga ataaccagtt tagagaggaa tacaaatgac atgatggagc 60
tgagaaacac agcgtgagag cttcatgatt caaacacaag tatcaatagc caaatcaacc 120
<210> 199
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 199
ccagtttaga gaggaataca aatgacatga tggagctgag aaacacagcg tgagagcttc 60
atgattcaaa cacaagtatc aatagccaaa tcaaccaggc agaagaaaga gtatcagagc 120
<210> 200
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 200
acatgatgga gctgagaaac acagcgtgag agcttcatga ttcaaacaca agtatcaata 60
gccaaatcaa ccaggcagaa gaaagagtat cagagcttga agactatctt gctaaaataa 120
<210> 201
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 201
cgtgagagct tcatgattca aacacaagta tcaatagcca aatcaaccag gcagaagaaa 60
gagtatcaga gcttgaagac tatcttgcta aaataaggca ggtagaaaag attacagaaa 120
<210> 202
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 202
caagtatcaa tagccaaatc aaccaggcag aagaaagagt atcagagctt gaagactatc 60
ttgctaaaat aaggcaggta gaaaagatta cagaaaaaaa taaaaagcaa tgaacaatac 120
<210> 203
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 203
aggcagaaga aagagtatca gagcttgaag actatcttgc taaaataagg caggtagaaa 60
agattacaga aaaaaataaa aagcaatgaa caataccttc cagaactatg ggactatgta 120
<210> 204
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 204
ttgaagacta tcttgctaaa ataaggcagg tagaaaagat tacagaaaaa aataaaaagc 60
aatgaacaat accttccaga actatgggac tatgtaaaaa gaccaaacct atgactgatt 120
<210> 205
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 205
ggcaggtaga aaagattaca gaaaaaaata aaaagcaatg aacaatacct tccagaacta 60
tgggactatg taaaaagacc aaacctatga ctgattgaag tacctgaaag agatgtggag 120
<210> 206
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 206
aaaataaaaa gcaatgaaca ataccttcca gaactatggg actatgtaaa aagaccaaac 60
ctatgactga ttgaagtacc tgaaagagat gtggagaatg gagccaagtc aaaaaacaca 120
<210> 207
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 207
atcatgtggg cgctcacaca gtgttggatt ttagagcatt tcaaaattca aatttttgga 60
ttaaggattc tcagcctgta aacctagagt tcttgactca aatggacttt cagtaccctt 120
<210> 208
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 208
tggattttag agcatttcaa aattcaaatt tttggattaa ggattctcag cctgtaaacc 60
tagagttctt gactcaaatg gactttcagt accctttcct caaccttccc acactcccga 120
<210> 209
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 209
caaatttttg gattaaggat tctcagcctg taaacctaga gttcttgact caaatggact 60
ttcagtaccc tttcctcaac cttcccacac tcccgaccat acacatgtac ctgcccacag 120
<210> 210
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 210
agcctgtaaa cctagagttc ttgactcaaa tggactttca gtaccctttc ctcaaccttc 60
ccacactccc gaccatacac atgtacctgc ccacagacat tcatacagtt tccttatttt 120
<210> 211
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 211
ctcaaatgga ctttcagtac cctttcctca accttcccac actcccgacc atacacatgt 60
acctgcccac agacattcat acagtttcct tattttaatg tcctgggaag aggtagaaac 120
<210> 212
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 212
tccctgagca ccctaggcgt tggggggacg ccctgtgagt gtgagagacc tccaagagcg 60
gctggagtct gaggcgctgg ccgggcttcg tgaattagac ctttatccta agctccttcc 120
<210> 213
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 213
gggacgccct gtgagtgtga gagacctcca agagcggctg gagtctgagg cgctggccgg 60
gcttcgtgaa ttagaccttt atcctaagct ccttccctct gccgggcact cggtgtcccc 120
<210> 214
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 214
atattgaaaa acaccagatg atctgcttgg aagggatata aacaggccag gcggatgcaa 60
gttaataaaa cctgaaaaag cccaggcagg aacacttaaa atgccccaga aacaaagcat 120
<210> 215
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 215
gcttggaagg gatataaaca ggccaggcgg atgcaagtta ataaaacctg aaaaagccca 60
ggcaggaaca cttaaaatgc cccagaaaca aagcatacca tgacaaattc tcacccaaca 120
<210> 216
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 216
agcctcaatg acgacctaag ctgcactttt ccccctagtt gtgtcttgcc atgctaaagg 60
acgtcacatt gcacaatctt aataaggttt ccaatcagcc ccacccgctc tggccccacc 120
<210> 217
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 217
acttttcccc ctagttgtgt cttgccatgc taaaggacgt cacattgcac aatcttaata 60
aggtttccaa tcagccccac ccgctctggc cccaccctca ccctccaaca aagatttatc 120
<210> 218
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 218
gtttcaaatg gttgcattta aggtcagaat ctttgtgttg ggaaatgcta gcgactgagc 60
cttgacagct gagcacgggt tgcctcatcc ctctcatgct gtctatttct taatctaaca 120
<210> 219
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 219
cagaatcttt gtgttgggaa atgctagcga ctgagccttg acagctgagc acgggttgcc 60
tcatccctct catgctgtct atttcttaat ctaacaactg ggcaatgcgt taaactggct 120
<210> 220
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 220
tagcgactga gccttgacag ctgagcacgg gttgcctcat ccctctcatg ctgtctattt 60
cttaatctaa caactgggca atgcgttaaa ctggcttttt tgacttccca gaacaatatc 120
<210> 221
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 221
gcacgggttg cctcatccct ctcatgctgt ctatttctta atctaacaac tgggcaatgc 60
gttaaactgg cttttttgac ttcccagaac aatatctaat tagcaaataa cacaattcag 120
<210> 222
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 222
tgctgtctat ttcttaatct aacaactggg caatgcgtta aactggcttt tttgacttcc 60
cagaacaata tctaattagc aaataacaca attcagtgac attcagcagg atgcaaattc 120
<210> 223
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 223
actgggcaat gcgttaaact ggcttttttg acttcccaga acaatatcta attagcaaat 60
aacacaattc agtgacattc agcaggatgc aaattccaga cactgcaatc atgaacactg 120
<210> 224
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 224
tttttgactt cccagaacaa tatctaatta gcaaataaca caattcagtg acattcagca 60
ggatgcaaat tccagacact gcaatcatga acactgtgaa gacagtcttc tccgtgggcc 120
<210> 225
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 225
taattagcaa ataacacaat tcagtgacat tcagcaggat gcaaattcca gacactgcaa 60
tcatgaacac tgtgaagaca gtcttctccg tgggccggga cacaaagcag tccacagtgt 120
<210> 226
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 226
tgacattcag caggatgcaa attccagaca ctgcaatcat gaacactgtg aagacagtct 60
tctccgtggg ccgggacaca aagcagtcca cagtgttggg acaaggccag gcgttgcact 120
<210> 227
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 227
cagacactgc aatcatgaac actgtgaaga cagtcttctc cgtgggccgg gacacaaagc 60
agtccacagt gttgggacaa ggccaggcgt tgcacttcac cagccgctgc atggagaagc 120
<210> 228
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 228
tgaagacagt cttctccgtg ggccgggaca caaagcagtc cacagtgttg ggacaaggcc 60
aggcgttgca cttcaccagc cgctgcatgg agaagccgtc gtacatgaca tagaagacgt 120
<210> 229
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 229
gggacacaaa gcagtccaca gtgttgggac aaggccaggc gttgcacttc accagccgct 60
gcatggagaa gccgtcgtac atgacataga agacgtacat gaaggcggct tcgaagatga 120
<210> 230
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 230
tgggacaagg ccaggcgttg cacttcacca gccgctgcat ggagaagccg tcgtacatga 60
catagaagac gtacatgaag gcggcttcga agatgacccg gaagaagatg ctgcttgtgt 120
<210> 231
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 231
tcaccagccg ctgcatggag aagccgtcgt acatgacata gaagacgtac atgaaggcgg 60
cttcgaagat gacccggaag aagatgctgc ttgtgtaggt ccaccacagg gagccttcga 120
<210> 232
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 232
cgtcgtacat gacatagaag acgtacatga aggcggcttc gaagatgacc cggaagaaga 60
tgctgcttgt gtaggtccac cacagggagc cttcgatgcg gaccttctgg gttttgatct 120
<210> 233
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 233
acatgaaggc ggcttcgaag atgacccgga agaagatgct gcttgtgtag gtccaccaca 60
gggagccttc gatgcggacc ttctgggttt tgatctcctc gatgtcctta aattcactct 120
<210> 234
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 234
cccggaagaa gatgctgctt gtgtaggtcc accacaggga gccttcgatg cggaccttct 60
gggttttgat ctcctcgatg tccttaaatt cactctttat ctcccccttg atgaacttcc 120
<210> 235
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 235
aggtccacca cagggagcct tcgatgcgga ccttctgggt tttgatctcc tcgatgtcct 60
taaattcact ctttatctcc cccttgatga acttcctctt cttctcatgt ctccggtagg 120
<210> 236
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 236
tgcggacctt ctgggttttg atctcctcga tgtccttaaa ttcactcttt atctccccct 60
tgatgaactt cctcttcttc tcatgtctcc ggtaggccac gtgcatggcc actaggagcg 120
<210> 237
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 237
cctcgatgtc cttaaattca ctctttatct cccccttgat gaacttcctc ttcttctcat 60
gtctccggta ggccacgtgc atggccacta ggagcgctgg cgtggacacg aagatcagct 120
<210> 238
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 238
ttatctcccc cttgatgaac ttcctcttct tctcatgtct ccggtaggcc acgtgcatgg 60
ccactaggag cgctggcgtg gacacgaaga tcagctgcag ggcccatagc cggatgtggg 120
<210> 239
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 239
tcttcttctc atgtctccgg taggccacgt gcatggccac taggagcgct ggcgtggaca 60
cgaagatcag ctgcagggcc catagccgga tgtgggagat ggggaagtag tgatcgtagc 120
<210> 240
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 240
ccacgtgcat ggccactagg agcgctggcg tggacacgaa gatcagctgc agggcccata 60
gccggatgtg ggagatgggg aagtagtgat cgtagcacac gttcttgcag cctggctgca 120
<210> 241
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 241
ctggcgtgga cacgaagatc agctgcaggg cccatagccg gatgtgggag atggggaagt 60
agtgatcgta gcacacgttc ttgcagcctg gctgcagggt gttgcagaca aagtcggcct 120
<210> 242
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 242
gcagggccca tagccggatg tgggagatgg ggaagtagtg atcgtagcac acgttcttgc 60
agcctggctg cagggtgttg cagacaaagt cggcctgctc atctccccac acctcctttg 120
<210> 243
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 243
agatggggaa gtagtgatcg tagcacacgt tcttgcagcc tggctgcagg gtgttgcaga 60
caaagtcggc ctgctcatct ccccacacct cctttgcagc cacaacgagg atcataatgc 120
<210> 244
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 244
acacgttctt gcagcctggc tgcagggtgt tgcagacaaa gtcggcctgc tcatctcccc 60
acacctcctt tgcagccaca acgaggatca taatgcgaaa aatgaagagg acggtgagcc 120
<210> 245
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 245
gggtgttgca gacaaagtcg gcctgctcat ctccccacac ctcctttgca gccacaacga 60
ggatcataat gcgaaaaatg aagaggacgg tgagccagat ctttccaatg ctggtggagt 120
<210> 246
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 246
gctcatctcc ccacacctcc tttgcagcca caacgaggat cataatgcga aaaatgaaga 60
ggacggtgag ccagatcttt ccaatgctgg tggagtgttt gttcacaccc cccaggatcg 120
<210> 247
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 247
cagccacaac gaggatcata atgcgaaaaa tgaagaggac ggtgagccag atctttccaa 60
tgctggtgga gtgtttgttc acacccccca ggatcgtctg cagcgtgccc caatccatct 120
<210> 248
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 248
gaaaaatgaa gaggacggtg agccagatct ttccaatgct ggtggagtgt ttgttcacac 60
cccccaggat cgtctgcagc gtgccccaat ccatcttcta ctctgggcgg tttgctctgg 120
<210> 249
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 249
agatctttcc aatgctggtg gagtgtttgt tcacaccccc caggatcgtc tgcagcgtgc 60
cccaatccat cttctactct gggcggtttg ctctggaaaa gacgaatgca cacaacacag 120
<210> 250
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 250
ctcccctcca tttagagccg ggcgccaatc tcagccaagc ttgttgcaaa tatgctatct 60
gtagcaggtg cagatcatat tatcaccatg gacctacatg cttctcaaat tcaggtatca 120
<210> 251
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 251
ccaatctcag ccaagcttgt tgcaaatatg ctatctgtag caggtgcaga tcatattatc 60
accatggacc tacatgcttc tcaaattcag gtatcagtgg aagctaaata ttggtgtttg 120
<210> 252
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 252
tcccgtggtc cagcccagcc ttctccactg tttttttccc tcttgcacat agttagaatc 60
ttaagtcagt gtcacacaat gtgctgtgca tctggcacaa cgataaacag cccgagggag 120
<210> 253
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 253
ccactgtttt tttccctctt gcacatagtt agaatcttaa gtcagtgtca cacaatgtgc 60
tgtgcatctg gcacaacgat aaacagcccg agggagggtt ggggacctaa gtgtcctaga 120
<210> 254
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 254
atgccatact tcaggcccca cccaatacct attgagcaac agagctcacc caatatctat 60
tgagcaaaaa tgtgttttca aacaagattc cagatgatgc atgtgctctt taaaatttca 120
<210> 255
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 255
atacctattg agcaacagag ctcacccaat atctattgag caaaaatgtg ttttcaaaca 60
agattccaga tgatgcatgt gctctttaaa atttcagaag cattgctgta agtcatgtat 120
<210> 256
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 256
cccaatatct attgagcaaa aatgtgtttt caaacaagat tccagatgat gcatgtgctc 60
tttaaaattt cagaagcatt gctgtaagtc atgtatgact atgagtagtt aattctctgc 120
<210> 257
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 257
tgttttcaaa caagattcca gatgatgcat gtgctcttta aaatttcaga agcattgctg 60
taagtcatgt atgactatga gtagttaatt ctctgctttc catctttatc tgctcctcat 120
<210> 258
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 258
atgcatgtgc tctttaaaat ttcagaagca ttgctgtaag tcatgtatga ctatgagtag 60
ttaattctct gctttccatc tttatctgct cctcatggtt tcttctggag aacccggact 120
<210> 259
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 259
gaagcattgc tgtaagtcat gtatgactat gagtagttaa ttctctgctt tccatcttta 60
tctgctcctc atggtttctt ctggagaacc cggactatga atgaattact ttcatttcaa 120
<210> 260
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 260
gactatgagt agttaattct ctgctttcca tctttatctg ctcctcatgg tttcttctgg 60
agaacccgga ctatgaatga attactttca tttcaataag ccttaggtct tgagagtatt 120
<210> 261
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 261
tgacctagct gtcatcaaac agcttgaaat aaagcagtac ctggagtccc acctggctat 60
tcttcctttt accgctccct gatgggtagc atggtcatgc cctctcacag gtgagaaaaa 120
<210> 262
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 262
tgaaataaag cagtacctgg agtcccacct ggctattctt ccttttaccg ctccctgatg 60
ggtagcatgg tcatgccctc tcacaggtga gaaaaaggag actcacaaag gttggatttg 120
<210> 263
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 263
ccacctggct attcttcctt ttaccgctcc ctgatgggta gcatggtcat gccctctcac 60
aggtgagaaa aaggagactc acaaaggttg gatttgttgc ttgagagcat atagctctca 120
<210> 264
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 264
cgctccctga tgggtagcat ggtcatgccc tctcacaggt gagaaaaagg agactcacaa 60
aggttggatt tgttgcttga gagcatatag ctctcataca ggggatgagg acaggtacct 120
<210> 265
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 265
atgccctctc acaggtgaga aaaaggagac tcacaaaggt tggatttgtt gcttgagagc 60
atatagctct catacagggg atgaggacag gtaccttggt ctgtacaggc tgctataaca 120
<210> 266
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 266
ggagactcac aaaggttgga tttgttgctt gagagcatat agctctcata caggggatga 60
ggacaggtac cttggtctgt acaggctgct ataacaagac accataactg ggtgggttaa 120
<210> 267
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 267
ttgcttgaga gcatatagct ctcatacagg ggatgaggac aggtaccttg gtctgtacag 60
gctgctataa caagacacca taactgggtg ggttaaacaa tggagattta tttactcaca 120
<210> 268
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 268
tacaggggat gaggacaggt accttggtct gtacaggctg ctataacaag acaccataac 60
tgggtgggtt aaacaatgga gatttattta ctcacagttc tggagactgg aaagtccaaa 120
<210> 269
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 269
tggtctgtac aggctgctat aacaagacac cataactggg tgggttaaac aatggagatt 60
tatttactca cagttctgga gactggaaag tccaaagtgc ctgtcaatta gatgctggtg 120
<210> 270
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 270
agacaccata actgggtggg ttaaacaatg gagatttatt tactcacagt tctggagact 60
ggaaagtcca aagtgcctgt caattagatg ctggtgaggc ctctcttcct ggcttgcaga 120
<210> 271
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 271
acaatggaga tttatttact cacagttctg gagactggaa agtccaaagt gcctgtcaat 60
tagatgctgg tgaggcctct cttcctggct tgcagaaggc caccttcttg ctctgtcctt 120
<210> 272
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 272
gttctggaga ctggaaagtc caaagtgcct gtcaattaga tgctggtgag gcctctcttc 60
ctggcttgca gaaggccacc ttcttgctct gtccttacat ggcagagaaa gagagagcga 120
<210> 273
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 273
gtgcctgtca attagatgct ggtgaggcct ctcttcctgg cttgcagaag gccaccttct 60
tgctctgtcc ttacatggca gagaaagaga gagcgagctc tctggtgtct cttataaaga 120
<210> 274
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 274
aggcctctct tcctggcttg cagaaggcca ccttcttgct ctgtccttac atggcagaga 60
aagagagagc gagctctctg gtgtctctta taaagaagga cactccttct ataggatcag 120
<210> 275
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 275
cttttcccac cctttttttt tttttttttt tttttagaca gcgtctcagt ctgtcaccca 60
ggctggagcg cggtggtacg atctcggctc attgcagcct ctgcctcccg agttcaagtg 120
<210> 276
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 276
tttttttttt tagacagcgt ctcagtctgt cacccaggct ggagcgcggt ggtacgatct 60
cggctcattg cagcctctgc ctcccgagtt caagtgagtc tcatgcctca gcctcatgag 120
<210> 277
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 277
gtctgtcacc caggctggag cgcggtggta cgatctcggc tcattgcagc ctctgcctcc 60
cgagttcaag tgagtctcat gcctcagcct catgagtagc tggggttaca ggtatgcaca 120
<210> 278
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 278
gtggtacgat ctcggctcat tgcagcctct gcctcccgag ttcaagtgag tctcatgcct 60
cagcctcatg agtagctggg gttacaggta tgcacaacca tgcctggcta atttttgtat 120
<210> 279
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 279
gcctctgcct cccgagttca agtgagtctc atgcctcagc ctcatgagta gctggggtta 60
caggtatgca caaccatgcc tggctaattt ttgtattttt agttgagtcg gggtttcatc 120
<210> 280
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 280
agtctcatgc ctcagcctca tgagtagctg gggttacagg tatgcacaac catgcctggc 60
taatttttgt atttttagtt gagtcggggt ttcatcatgt tggccaggct ggtctcgaac 120
<210> 281
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 281
tagctggggt tacaggtatg cacaaccatg cctggctaat ttttgtattt ttagttgagt 60
cggggtttca tcatgttggc caggctggtc tcgaacccct aacctcaagt gatccacctg 120
<210> 282
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 282
accatgcctg gctaattttt gtatttttag ttgagtcggg gtttcatcat gttggccagg 60
ctggtctcga acccctaacc tcaagtgatc cacctgcctt ggcctcccaa agtcctggga 120
<210> 283
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 283
ttttagttga gtcggggttt catcatgttg gccaggctgg tctcgaaccc ctaacctcaa 60
gtgatccacc tgccttggcc tcccaaagtc ctgggattac agttgtgtgc caccgtgctt 120
<210> 284
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 284
atgttggcca ggctggtctc gaacccctaa cctcaagtga tccacctgcc ttggcctccc 60
aaagtcctgg gattacagtt gtgtgccacc gtgcttggcc cacttttccc tcttttgaca 120
<210> 285
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 285
ccctaacctc aagtgatcca cctgccttgg cctcccaaag tcctgggatt acagttgtgt 60
gccaccgtgc ttggcccact tttccctctt ttgacagaga tgtgagttca agaaatactt 120
<210> 286
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 286
ccttggcctc ccaaagtcct gggattacag ttgtgtgcca ccgtgcttgg cccacttttc 60
cctcttttga cagagatgtg agttcaagaa atactttgct actcttttaa ctctatcaaa 120
<210> 287
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 287
ttacagttgt gtgccaccgt gcttggccca cttttccctc ttttgacaga gatgtgagtt 60
caagaaatac tttgctactc ttttaactct atcaaagaga aaagggaaag taactcaagt 120
<210> 288
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 288
ggcccacttt tccctctttt gacagagatg tgagttcaag aaatactttg ctactctttt 60
aactctatca aagagaaaag ggaaagtaac tcaagtccca cagttactta tagccatgga 120
<210> 289
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 289
ggacatgata ggcgaatctc taaataaatg tgaaatgaat gaacgctaca tcttgtcttt 60
taccattaca gcatcattga ggggataacc attatgctgt cttggtgctt gtattaatcg 120
<210> 290
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 290
taaatgtgaa atgaatgaac gctacatctt gtcttttacc attacagcat cattgagggg 60
ataaccatta tgctgtcttg gtgcttgtat taatcgagtc aagttcccta acattggttt 120
<210> 291
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 291
catctctcat tatgagaaaa tgggaaaact tcctgaaaaa aaaaaaagta gagtcatctt 60
gctctatttt attcctcgct ttgttttagt gtgaaaagta gcttgttcca gttcagtttg 120
<210> 292
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 292
aaaacttcct gaaaaaaaaa aaagtagagt catcttgctc tattttattc ctcgctttgt 60
tttagtgtga aaagtagctt gttccagttc agtttgggaa tggaggttac tgatgcctga 120
<210> 293
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 293
aagatttgct gggcatggtg acacacacct gtaatcctag ctacttggga ggctgaggtg 60
ggaggatggc ttgaaccctg gcagtgtagg ctgcaatgag ccatgattgg gccactggac 120
<210> 294
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 294
acacctgtaa tcctagctac ttgggaggct gaggtgggag gatggcttga accctggcag 60
tgtaggctgc aatgagccat gattgggcca ctggactcct gcctgggcga cagggtaaga 120
<210> 295
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 295
gaggctgagg tgggaggatg gcttgaaccc tggcagtgta ggctgcaatg agccatgatt 60
gggccactgg actcctgcct gggcgacagg gtaagaccct gcctcactct gtcaccccgg 120
<210> 296
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 296
gaaccctggc agtgtaggct gcaatgagcc atgattgggc cactggactc ctgcctgggc 60
gacagggtaa gaccctgcct cactctgtca ccccggctgg cgtgcagtgg tgagatcttg 120
<210> 297
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 297
tgagccatga ttgggccact ggactcctgc ctgggcgaca gggtaagacc ctgcctcact 60
ctgtcacccc ggctggcgtg cagtggtgag atcttggctc actgcaacct ctgcctccca 120
<210> 298
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 298
tcctgcctgg gcgacagggt aagaccctgc ctcactctgt caccccggct ggcgtgcagt 60
ggtgagatct tggctcactg caacctctgc ctcccaggtt caagcgattc tcatgcctca 120
<210> 299
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 299
ccctgcctca ctctgtcacc ccggctggcg tgcagtggtg agatcttggc tcactgcaac 60
ctctgcctcc caggttcaag cgattctcat gcctcagcct ccctagtagg ctgggattac 120
<210> 300
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 300
ctggcgtgca gtggtgagat cttggctcac tgcaacctct gcctcccagg ttcaagcgat 60
tctcatgcct cagcctccct agtaggctgg gattacaggc gtactccacc acgcctggct 120
<210> 301
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 301
gacaaggttt gacttccagc ccagcctctt ttccacaacc tgctaaatcc tgatccatct 60
gaaaactttt ctaattagtg aagatgacta ataaaaattt tccctatctc caaggtagga 120
<210> 302
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 302
cctcttttcc acaacctgct aaatcctgat ccatctgaaa acttttctaa ttagtgaaga 60
tgactaataa aaattttccc tatctccaag gtaggagctt tctggaagtt tctagaaatt 120
<210> 303
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 303
cctgatccat ctgaaaactt ttctaattag tgaagatgac taataaaaat tttccctatc 60
tccaaggtag gagctttctg gaagtttcta gaaattttca ataaccacca gccaaggtta 120
<210> 304
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 304
atctgcatct ttgcaaccaa cttcctccct tgcctctctg cttttccatc ccacttttca 60
tgtgtcctcc ataccatcta taacagtgat ctccctggaa cactcaagaa gacacaacat 120
<210> 305
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 305
ctcccttgcc tctctgcttt tccatcccac ttttcatgtg tcctccatac catctataac 60
agtgatctcc ctggaacact caagaagaca caacatacca tattatttaa agaccagggt 120
<210> 306
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 306
tcccactttt catgtgtcct ccataccatc tataacagtg atctccctgg aacactcaag 60
aagacacaac ataccatatt atttaaagac cagggtactg gacagtggct cacacctgta 120
<210> 307
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 307
accatctata acagtgatct ccctggaaca ctcaagaaga cacaacatac catattattt 60
aaagaccagg gtactggaca gtggctcaca cctgtattcc cgactttgag agtctgaagc 120
<210> 308
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 308
ggaacactca agaagacaca acataccata ttatttaaag accagggtac tggacagtgg 60
ctcacacctg tattcccgac tttgagagtc tgaagcggga ggatcacttg aggccaggag 120
<210> 309
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 309
accatattat ttaaagacca gggtactgga cagtggctca cacctgtatt cccgactttg 60
agagtctgaa gcgggaggat cacttgaggc caggagttaa gagaccagcc tgggcaacac 120
<210> 310
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 310
actggacagt ggctcacacc tgtattcccg actttgagag tctgaagcgg gaggatcact 60
tgaggccagg agttaagaga ccagcctggg caacacagca agaccctgtc tctaaaaaaa 120
<210> 311
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 311
ccctctgtcc tgattcatgc ctcttgccac agcataattc ctcgtagcac ccacttttac 60
tttcagagct gccctggttt ttggatgaca aaatatacga ataccttcag taccccagag 120
<210> 312
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 312
tgccacagca taattcctcg tagcacccac ttttactttc agagctgccc tggtttttgg 60
atgacaaaat atacgaatac cttcagtacc ccagagattg cttatgagaa ttaaatcaaa 120
<210> 313
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 313
acccactttt actttcagag ctgccctggt ttttggatga caaaatatac gaataccttc 60
agtaccccag agattgctta tgagaattaa atcaaataac tcatctcact tctgctgatt 120
<210> 314
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 314
cctggttttt ggatgacaaa atatacgaat accttcagta ccccagagat tgcttatgag 60
aattaaatca aataactcat ctcacttctg ctgattcacg gggtacctca ggcaagttgc 120
<210> 315
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 315
acgaatacct tcagtacccc agagattgct tatgagaatt aaatcaaata actcatctca 60
cttctgctga ttcacggggt acctcaggca agttgctttt tccatctcag cttcagttcg 120
<210> 316
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 316
acctgcctgc cactcagtat ttctgccagt ctctcgtgcc agccaaacca tcttcctccc 60
acaaaaccat cttcctattc cccaacccat taacctgcca tgacccatta acccacacac 120
<210> 317
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 317
gccagtctct cgtgccagcc aaaccatctt cctcccacaa aaccatcttc ctattcccca 60
acccattaac ctgccatgac ccattaaccc acacacatat cctgttgacc tgttgctgta 120
<210> 318
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 318
catcttcctc ccacaaaacc atcttcctat tccccaaccc attaacctgc catgacccat 60
taacccacac acatatcctg ttgacctgtt gctgtaccca atcatacatc ctccatccac 120
<210> 319
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 319
gtaaagtagg tgctcagtga agttcaacac cctttccctg gttcaggctt tgctgtggcc 60
aggagttggc cctgggccag caactagtca cctaccccac cctgcctctt tttttctcac 120
<210> 320
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 320
caacaccctt tccctggttc aggctttgct gtggccagga gttggccctg ggccagcaac 60
tagtcaccta ccccaccctg cctctttttt tctcaccact aaaaagtagg ggttgttcta 120
<210> 321
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 321
tttgctgtgg ccaggagttg gccctgggcc agcaactagt cacctacccc accctgcctc 60
tttttttctc accactaaaa agtaggggtt gttctaagag ctgttctctc tgtgccccag 120
<210> 322
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 322
gtccacctct gaggtctcta tgtcacaagg ccagacacag gggtgcacta aaaaaatcta 60
ttgagcacct actgtgtact ggatacaaag ataaacaaat aggaaactcg gttcagaggg 120
<210> 323
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 323
acaaggccag acacaggggt gcactaaaaa aatctattga gcacctactg tgtactggat 60
acaaagataa acaaatagga aactcggttc agagggagac aacaacatgt aaacaagtac 120
<210> 324
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 324
tcctgacttg gggcacctct gtctaattcc accagcacag ccactcacta tgtgctcatc 60
tcactcctcc agcagccgct gtaggacttg gggctgggca cccccccacc cccgcctcca 120
<210> 325
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 325
aattccacca gcacagccac tcactatgtg ctcatctcac tcctccagca gccgctgtag 60
gacttggggc tgggcacccc cccacccccg cctccagcct ccttgcgcgc cccagcaccc 120
<210> 326
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 326
atattgaatg gaatttataa ttgtcagtgt caatcatgcc aagaattgaa ataactattt 60
aatttccttt cccccccttt tccttcccat cagatgataa tatttgaggg gtttggatgg 120
<210> 327
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 327
cagtgtcaat catgccaaga attgaaataa ctatttaatt tcctttcccc cccttttcct 60
tcccatcaga tgataatatt tgaggggttt ggatggcttt agctatacat tgtttaatta 120
<210> 328
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 328
aaataactat ttaatttcct ttccccccct tttccttccc atcagatgat aatatttgag 60
gggtttggat ggctttagct atacattgtt taattattcc atcctaccca agcatacaga 120
<210> 329
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 329
cccccttttc cttcccatca gatgataata tttgaggggt ttggatggct ttagctatac 60
attgtttaat tattccatcc tacccaagca tacagatgtt agtggatcaa atgaggcacg 120
<210> 330
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 330
ataatatttg aggggtttgg atggctttag ctatacattg tttaattatt ccatcctacc 60
caagcataca gatgttagtg gatcaaatga ggcacgtgaa aagtatctct ctataaatgt 120
<210> 331
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 331
ctttagctat acattgttta attattccat cctacccaag catacagatg ttagtggatc 60
aaatgaggca cgtgaaaagt atctctctat aaatgtaaaa tatgcaaatg tttacatgtt 120
<210> 332
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 332
ttccatccta cccaagcata cagatgttag tggatcaaat gaggcacgtg aaaagtatct 60
ctctataaat gtaaaatatg caaatgttta catgttgaca gtgataggct ctactacttg 120
<210> 333
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 333
tgttagtgga tcaaatgagg cacgtgaaaa gtatctctct ataaatgtaa aatatgcaaa 60
tgtttacatg ttgacagtga taggctctac tacttgctgt ataagcgaga ccaaagtcca 120
<210> 334
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 334
tgaaaagtat ctctctataa atgtaaaata tgcaaatgtt tacatgttga cagtgatagg 60
ctctactact tgctgtataa gcgagaccaa agtccaggcc aatggataat aatgaggttg 120
<210> 335
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 335
aaaatatgca aatgtttaca tgttgacagt gataggctct actacttgct gtataagcga 60
gaccaaagtc caggccaatg gataataatg aggttgtgga cctaactagg gggagcctaa 120
<210> 336
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 336
gacagtgata ggctctacta cttgctgtat aagcgagacc aaagtccagg ccaatggata 60
ataatgaggt tgtggaccta actaggggga gcctaaaata atgttgggac tacctagatg 120
<210> 337
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 337
ataaaaacgt gcctagaaaa aaattgaagg ttaccaatat attaaatgct gtggaatcaa 60
cgatttttat gtttctcctt gttacttcca agtatgcttt aaatctgcag tgcgtatgtt 120
<210> 338
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 338
tgaaggttac caatatatta aatgctgtgg aatcaacgat ttttatgttt ctccttgtta 60
cttccaagta tgctttaaat ctgcagtgcg tatgttttat gtcttacatt aaaaacaatt 120
<210> 339
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 339
ccacggagca ccattacctg tcaccattac ctgaatggct atactgcttg ctttcatttt 60
ggtagagtgg aaaggttacc taggtttcag tgcttgaaaa gatttcagaa agcagtagta 120
<210> 340
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 340
cattacctga atggctatac tgcttgcttt cattttggta gagtggaaag gttacctagg 60
tttcagtgct tgaaaagatt tcagaaagca gtagtacgtc tggttagact agaatcagtc 120
<210> 341
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 341
tacagcaggt gggtgttgtg ctgtgctggt gacccatact tgttcaccct agttagataa 60
acagtagagt agcccctaaa cgttaaaacc cctcaacttg tttttgtttt tgagaaaggg 120
<210> 342
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 342
gctggtgacc catacttgtt caccctagtt agataaacag tagagtagcc cctaaacgtt 60
aaaacccctc aacttgtttt tgtttttgag aaagggtctt gctctgtcgc tcaggctgga 120
<210> 343
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 343
ctagttagat aaacagtaga gtagccccta aacgttaaaa cccctcaact tgtttttgtt 60
tttgagaaag ggtcttgctc tgtcgctcag gctggagtgc agtggcgctg tgcgatcatg 120
<210> 344
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 344
cccctaaacg ttaaaacccc tcaacttgtt tttgtttttg agaaagggtc ttgctctgtc 60
gctcaggctg gagtgcagtg gcgctgtgcg atcatggctg accttagcct tgacctccca 120
<210> 345
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 345
cttgtttttg tttttgagaa agggtcttgc tctgtcgctc aggctggagt gcagtggcgc 60
tgtgcgatca tggctgacct tagccttgac ctcccaggct ccattgatcc tcatgccttg 120
<210> 346
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 346
tcttgctctg tcgctcaggc tggagtgcag tggcgctgtg cgatcatggc tgaccttagc 60
cttgacctcc caggctccat tgatcctcat gccttggccc gtagctggga ctacaggtac 120
<210> 347
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 347
gtgcagtggc gctgtgcgat catggctgac cttagccttg acctcccagg ctccattgat 60
cctcatgcct tggcccgtag ctgggactac aggtacacac caccacgcct ggctaatttt 120
<210> 348
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 348
gctgacctta gccttgacct cccaggctcc attgatcctc atgccttggc ccgtagctgg 60
gactacaggt acacaccacc acgcctggct aatttttgta tttttttcta gaggtggggt 120
<210> 349
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 349
ggctccattg atcctcatgc cttggcccgt agctgggact acaggtacac accaccacgc 60
ctggctaatt tttgtatttt tttctagagg tggggtttca tcatgttgcc caggctggtc 120
<210> 350
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 350
gcccgtagct gggactacag gtacacacca ccacgcctgg ctaatttttg tatttttttc 60
tagaggtggg gtttcatcat gttgcccagg ctggtcttga actgctgggc tcaagtggtc 120
<210> 351
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 351
acaccaccac gcctggctaa tttttgtatt tttttctaga ggtggggttt catcatgttg 60
cccaggctgg tcttgaactg ctgggctcaa gtggtctatc ctcctcgacc tcccaaagtg 120
<210> 352
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 352
tgtatttttt tctagaggtg gggtttcatc atgttgccca ggctggtctt gaactgctgg 60
gctcaagtgg tctatcctcc tcgacctccc aaagtgctgg gattacatgt gtgagccact 120
<210> 353
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 353
ttcatcatgt tgcccaggct ggtcttgaac tgctgggctc aagtggtcta tcctcctcga 60
cctcccaaag tgctgggatt acatgtgtga gccactgtgc ctgggaaaac cctcaacttt 120
<210> 354
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 354
ttgaactgct gggctcaagt ggtctatcct cctcgacctc ccaaagtgct gggattacat 60
gtgtgagcca ctgtgcctgg gaaaaccctc aacttttctt ttaaaaaaga ggtcaacttt 120
<210> 355
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 355
caagattttt atatcagagg ccttgggaca tagctttgtt agctatgcca gtaattaaca 60
ggcataactc agtaactgag agtttaccct ttggtacttc tgaaatcagg tgcagcccca 120
<210> 356
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 356
gggacatagc tttgttagct atgccagtaa ttaacaggca taactcagta actgagagtt 60
taccctttgg tacttctgaa atcaggtgca gccccatctt tcttcccaga gcattagtgt 120
<210> 357
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 357
agctgttgtc ccaagttatc ctggggaact gcatctggtt cttgcaaaac accaagtaga 60
caggctctct tttacctccc cttgagggca ttaacattca gtagtcactt ccattcagtt 120
<210> 358
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 358
ggaactgcat ctggttcttg caaaacacca agtagacagg ctctctttta cctccccttg 60
agggcattaa cattcagtag tcacttccat tcagttaacc ctttattttt atggtttttc 120
<210> 359
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 359
atcaagtggt ctaagctatc ccctgactct ggggccctcc tgggtatcag aagacttgca 60
cttgagtcca atgcctacca tcagttatcc tcatgctctc ccagtcagtt attcagttat 120
<210> 360
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 360
gactctgggg ccctcctggg tatcagaaga cttgcacttg agtccaatgc ctaccatcag 60
ttatcctcat gctctcccag tcagttattc agttatctca gagtgcaaag acggaagaac 120
<210> 361
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 361
agaagacttg cacttgagtc caatgcctac catcagttat cctcatgctc tcccagtcag 60
ttattcagtt atctcagagt gcaaagacgg aagaacacga cagttcagat aaaaagcctc 120
<210> 362
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 362
gcctaccatc agttatcctc atgctctccc agtcagttat tcagttatct cagagtgcaa 60
agacggaaga acacgacagt tcagataaaa agcctcattt aaaatctgga atgctaccct 120
<210> 363
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 363
tctcccagtc agttattcag ttatctcaga gtgcaaagac ggaagaacac gacagttcag 60
ataaaaagcc tcatttaaaa tctggaatgc tacccttatg ggccatgtga agttgagcaa 120
<210> 364
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 364
ctcagagtgc aaagacggaa gaacacgaca gttcagataa aaagcctcat ttaaaatctg 60
gaatgctacc cttatgggcc atgtgaagtt gagcaaatcc ttagtcaatc gaggcctccg 120
<210> 365
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 365
acgacagttc agataaaaag cctcatttaa aatctggaat gctaccctta tgggccatgt 60
gaagttgagc aaatccttag tcaatcgagg cctccgtgtc atcattgtaa gatgtggcag 120
<210> 366
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 366
atttaaaatc tggaatgcta cccttatggg ccatgtgaag ttgagcaaat ccttagtcaa 60
tcgaggcctc cgtgtcatca ttgtaagatg tggcagtagt aactgtttac acatttgtgg 120
<210> 367
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 367
tatgggccat gtgaagttga gcaaatcctt agtcaatcga ggcctccgtg tcatcattgt 60
aagatgtggc agtagtaact gtttacacat ttgtggtaaa gattaaatga tgtttctcaa 120
<210> 368
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 368
atccttagtc aatcgaggcc tccgtgtcat cattgtaaga tgtggcagta gtaactgttt 60
acacatttgt ggtaaagatt aaatgatgtt tctcaaaatt ccaggctcat agaagttcct 120
<210> 369
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 369
tgtcatcatt gtaagatgtg gcagtagtaa ctgtttacac atttgtggta aagattaaat 60
gatgtttctc aaaattccag gctcatagaa gttcctcagc aatgaatgct tcccttgttt 120
<210> 370
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 370
tagtaactgt ttacacattt gtggtaaaga ttaaatgatg tttctcaaaa ttccaggctc 60
atagaagttc ctcagcaatg aatgcttccc ttgttttctt cataaaatga gaatcccagt 120
<210> 371
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 371
taaagattaa atgatgtttc tcaaaattcc aggctcatag aagttcctca gcaatgaatg 60
cttcccttgt tttcttcata aaatgagaat cccagtttct tgccatctga agaactatag 120
<210> 372
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 372
aattccaggc tcatagaagt tcctcagcaa tgaatgcttc ccttgttttc ttcataaaat 60
gagaatccca gtttcttgcc atctgaagaa ctataggacc attttggttt atcttttatc 120
<210> 373
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 373
atcatccact tgttaaaagg agatactctt tctgagatat caaactcacg tgaaccctcc 60
cttacccttc cccagccact gtctccaccc caactgggag attattttgg tgtcatatag 120
<210> 374
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 374
actctttctg agatatcaaa ctcacgtgaa ccctccctta cccttcccca gccactgtct 60
ccaccccaac tgggagatta ttttggtgtc atatagactg aacttcattc caagacagcc 120
<210> 375
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 375
cgtgaaccct cccttaccct tccccagcca ctgtctccac cccaactggg agattatttt 60
ggtgtcatat agactgaact tcattccaag acagcctgaa gtgtcagtga cagacctggg 120
<210> 376
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 376
cagccactgt ctccacccca actgggagat tattttggtg tcatatagac tgaacttcat 60
tccaagacag cctgaagtgt cagtgacaga cctgggactc tagcccagaa atgtggatgc 120
<210> 377
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 377
acaggccacc tttcaaagca tgatacatgc agacacatgt cttgtgtaca tgctgctcat 60
cacatctgga gctcttatcc tccttttttt ctaggttaat tctcagctca gttttcacct 120
<210> 378
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 378
acatgcagac acatgtcttg tgtacatgct gctcatcaca tctggagctc ttatcctcct 60
ttttttctag gttaattctc agctcagttt tcacctcctc ctccagacct tcccttacct 120
<210> 379
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 379
catgctgctc atcacatctg gagctcttat cctccttttt ttctaggtta attctcagct 60
cagttttcac ctcctcctcc agaccttccc ttacctacct cactgctatc acaaagtgct 120
<210> 380
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 380
tcttatcctc ctttttttct aggttaattc tcagctcagt tttcacctcc tcctccagac 60
cttcccttac ctacctcact gctatcacaa agtgctctag actgcaaggc ttagaaacag 120
<210> 381
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 381
taattctcag ctcagttttc acctcctcct ccagaccttc ccttacctac ctcactgcta 60
tcacaaagtg ctctagactg caaggcttag aaacagaaat ttatggactc acattcctga 120
<210> 382
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 382
cctcctccag accttccctt acctacctca ctgctatcac aaagtgctct agactgcaag 60
gcttagaaac agaaatttat ggactcacat tcctgaagtc tggaagtctg agatcaagat 120
<210> 383
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 383
acctcactgc tatcacaaag tgctctagac tgcaaggctt agaaacagaa atttatggac 60
tcacattcct gaagtctgga agtctgagat caagatgtct gcagaattga ttccttctga 120
<210> 384
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 384
ctagactgca aggcttagaa acagaaattt atggactcac attcctgaag tctggaagtc 60
tgagatcaag atgtctgcag aattgattcc ttctgaggac caagacagga ggatctgctc 120
<210> 385
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 385
attgtggacc ttccctacca aagggagtgt tgaaactcaa gtcccgccct ggctctttag 60
aatggaccac tgagagccac aggaccgttt tggggctgac ctgtcttatt acgtatgtac 120
<210> 386
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 386
gagtgttgaa actcaagtcc cgccctggct ctttagaatg gaccactgag agccacagga 60
ccgttttggg gctgacctgt cttattacgt atgtacttct aggttgcaag gttttgaaat 120
<210> 387
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 387
ttgtcttggg gaaaaaataa ccaccttttc tcacctgtga tactgtaggg tttcagaatg 60
gctttctgtt tgggaaagca agacttgctg ttaaaaggct acagtatata ttgtgactac 120
<210> 388
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 388
cttttctcac ctgtgatact gtagggtttc agaatggctt tctgtttggg aaagcaagac 60
ttgctgttaa aaggctacag tatatattgt gactacagga agacaatttg gtttaaaatc 120
<210> 389
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 389
aataaaagca gcaagccata ctgatcctca tccaatatgc caacagctca gatgctgcca 60
ttaataagga aacaaacctg taagttacta aataaaaaaa aaaatcaaca caataccatt 120
<210> 390
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 390
tcctcatcca atatgccaac agctcagatg ctgccattaa taaggaaaca aacctgtaag 60
ttactaaata aaaaaaaaaa tcaacacaat accatttata atatttcaac actacctcaa 120
<210> 391
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 391
cccggaaagc gggtggcagc ggggtccatg agggcgggcg ggcaggcgag agggtcctca 60
gctaaagcga cgtctccagg ctgtggatgg ggctccctgg acttgaagag gtagctgatt 120
<210> 392
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 392
tccatgaggg cgggcgggca ggcgagaggg tcctcagcta aagcgacgtc tccaggctgt 60
ggatggggct ccctggactt gaagaggtag ctgatttgct tgtgtcggtc gtcaggttga 120
<210> 393
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 393
agagggtcct cagctaaagc gacgtctcca ggctgtggat ggggctccct ggacttgaag 60
aggtagctga tttgcttgtg tcggtcgtca ggttgatccc actgtcgatg gcgctgttgt 120
<210> 394
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 394
tctccaggct gtggatgggg ctccctggac ttgaagaggt agctgatttg cttgtgtcgg 60
tcgtcaggtt gatcccactg tcgatggcgc tgttgttctt gttgagggat gacggcgggc 120
<210> 395
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 395
ctggacttga agaggtagct gatttgcttg tgtcggtcgt caggttgatc ccactgtcga 60
tggcgctgtt gttcttgttg agggatgacg gcgggctcga gttctgcttg agcgcggcat 120
<210> 396
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 396
tgcttgtgtc ggtcgtcagg ttgatcccac tgtcgatggc gctgttgttc ttgttgaggg 60
atgacggcgg gctcgagttc tgcttgagcg cggcatcttc ttccaggtcg gtgtcatcaa 120
<210> 397
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 397
tcccactgtc gatggcgctg ttgttcttgt tgagggatga cggcgggctc gagttctgct 60
tgagcgcggc atcttcttcc aggtcggtgt catcaatgag ggggatgtgg ggctgggaat 120
<210> 398
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 398
tcttgttgag ggatgacggc gggctcgagt tctgcttgag cgcggcatct tcttccaggt 60
cggtgtcatc aatgaggggg atgtggggct gggaatcttc gatccggaat tcaggatgag 120
<210> 399
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 399
tcgagttctg cttgagcgcg gcatcttctt ccaggtcggt gtcatcaatg agggggatgt 60
ggggctggga atcttcgatc cggaattcag gatgagccat gaagttatgg atggaggttc 120
<210> 400
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 400
cttcttccag gtcggtgtca tcaatgaggg ggatgtgggg ctgggaatct tcgatccgga 60
attcaggatg agccatgaag ttatggatgg aggttcgaga ttcaggcttt tctaaacctt 120
<210> 401
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 401
tgagggggat gtggggctgg gaatcttcga tccggaattc aggatgagcc atgaagttat 60
ggatggaggt tcgagattca ggcttttcta aaccttcata gagagagcta cggaacgcct 120
<210> 402
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 402
cttcgatccg gaattcagga tgagccatga agttatggat ggaggttcga gattcaggct 60
tttctaaacc ttcatagaga gagctacgga acgccttcac gacgcggatc tgcaagggaa 120
<210> 403
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 403
gtgacggctc cgcacctgct gaggcctgga gcctgcctcc cgggctgagg gctcagctct 60
ggcctccggc tggatgccgt ctagagtcct atgtgctcat aggtcttttt ctttcagtaa 120
<210> 404
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 404
cctggagcct gcctcccggg ctgagggctc agctctggcc tccggctgga tgccgtctag 60
agtcctatgt gctcataggt ctttttcttt cagtaaacag tataaaacat tactattttc 120
<210> 405
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 405
acaaaactag aatacacagt cttaagtggg gggaatatca taaaaattca ttacttcaat 60
gtttaacttt ttcacaatta cttgaaaatt catagcacag catcactgta acttgctaat 120
<210> 406
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 406
agtgggggga atatcataaa aattcattac ttcaatgttt aactttttca caattacttg 60
aaaattcata gcacagcatc actgtaactt gctaatcatg aaagctgtaa attaacaatc 120
<210> 407
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 407
acagctgcac gcgcggggtt tctctccgaa ccggagtgca gcgtagtcga ggtccaggat 60
tccccatcca ttattcacga tgtttactag agcggggcaa gggagaggaa agaagaggga 120
<210> 408
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 408
tccgaaccgg agtgcagcgt agtcgaggtc caggattccc catccattat tcacgatgtt 60
tactagagcg gggcaaggga gaggaaagaa gagggaagta gagagaaaaa gaggctaggg 120
<210> 409
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 409
ctattcccac caaaaaaatt ttggcgctgg gaaaatattg cagaagggcg ggcctgagtg 60
tacttgtgct gtccgtggtg ctgaaatttg ccctcgcgca ggggccgcac gacttcagag 120
<210> 410
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 410
cgctgggaaa atattgcaga agggcgggcc tgagtgtact tgtgctgtcc gtggtgctga 60
aatttgccct cgcgcagggg ccgcacgact tcagagcagg agccccaaag cagaggacag 120
<210> 411
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 411
gctcttctcg cggatccagt gttccgtttc cagcccccaa tctcagagcg gagccgacag 60
agagcaggga accggcatgg ccaaagccgc ggcgatcggc atcgacctgg gcaccaccta 120
<210> 412
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 412
cgtttccagc ccccaatctc agagcggagc cgacagagag cagggaaccg gcatggccaa 60
agccgcggcg atcggcatcg acctgggcac cacctactcc tgcgtggggg tgttccaaca 120
<210> 413
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 413
cggagccgac agagagcagg gaaccggcat ggccaaagcc gcggcgatcg gcatcgacct 60
gggcaccacc tactcctgcg tgggggtgtt ccaacacggc aaggtggaga tcatcgccaa 120
<210> 414
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 414
cggcatggcc aaagccgcgg cgatcggcat cgacctgggc accacctact cctgcgtggg 60
ggtgttccaa cacggcaagg tggagatcat cgccaacgac cagggcaacc gcaccacccc 120
<210> 415
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 415
cggcatcgac ctgggcacca cctactcctg cgtgggggtg ttccaacacg gcaaggtgga 60
gatcatcgcc aacgaccagg gcaaccgcac cacccccagc tacgtggcct tcacggacac 120
<210> 416
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 416
ctcctgcgtg ggggtgttcc aacacggcaa ggtggagatc atcgccaacg accagggcaa 60
ccgcaccacc cccagctacg tggccttcac ggacaccgag cggctcatcg gggatgcggc 120
<210> 417
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 417
cggcaaggtg gagatcatcg ccaacgacca gggcaaccgc accaccccca gctacgtggc 60
cttcacggac accgagcggc tcatcgggga tgcggccaag aaccaggtgg cgctgaaccc 120
<210> 418
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 418
cgaccagggc aaccgcacca cccccagcta cgtggccttc acggacaccg agcggctcat 60
cggggatgcg gccaagaacc aggtggcgct gaacccgcag aacaccgtgt ttgacgcgaa 120
<210> 419
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 419
cagctacgtg gccttcacgg acaccgagcg gctcatcggg gatgcggcca agaaccaggt 60
ggcgctgaac ccgcagaaca ccgtgtttga cgcgaagcgg ctgattggcc gcaagttcgg 120
<210> 420
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 420
cgagcggctc atcggggatg cggccaagaa ccaggtggcg ctgaacccgc agaacaccgt 60
gtttgacgcg aagcggctga ttggccgcaa gttcggcgac ccggtggtgc agtcggacat 120
<210> 421
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 421
caagaaccag gtggcgctga acccgcagaa caccgtgttt gacgcgaagc ggctgattgg 60
ccgcaagttc ggcgacccgg tggtgcagtc ggacatgaag cactggcctt tccaggtgat 120
<210> 422
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 422
gcagaacacc gtgtttgacg cgaagcggct gattggccgc aagttcggcg acccggtggt 60
gcagtcggac atgaagcact ggcctttcca ggtgatcaac gacggagaca agcccaaggt 120
<210> 423
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 423
gcggctgatt ggccgcaagt tcggcgaccc ggtggtgcag tcggacatga agcactggcc 60
tttccaggtg atcaacgacg gagacaagcc caaggtgcag gtgagctaca agggggagac 120
<210> 424
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 424
cgacccggtg gtgcagtcgg acatgaagca ctggcctttc caggtgatca acgacggaga 60
caagcccaag gtgcaggtga gctacaaggg ggagaccaag gcattctacc ccgaggagat 120
<210> 425
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 425
gaagcactgg cctttccagg tgatcaacga cggagacaag cccaaggtgc aggtgagcta 60
caagggggag accaaggcat tctaccccga ggagatctcg tccatggtgc tgaccaagat 120
<210> 426
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 426
caacgacgga gacaagccca aggtgcaggt gagctacaag ggggagacca aggcattcta 60
ccccgaggag atctcgtcca tggtgctgac caagatgaag gagatcgccg aggcgtacct 120
<210> 427
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 427
gcaggtgagc tacaaggggg agaccaaggc attctacccc gaggagatct cgtccatggt 60
gctgaccaag atgaaggaga tcgccgaggc gtacctgggc tacccggtga ccaacgcggt 120
<210> 428
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 428
caaggcattc taccccgagg agatctcgtc catggtgctg accaagatga aggagatcgc 60
cgaggcgtac ctgggctacc cggtgaccaa cgcggtgatc accgtgccgg cctacttcaa 120
<210> 429
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 429
ctcgtccatg gtgctgacca agatgaagga gatcgccgag gcgtacctgg gctacccggt 60
gaccaacgcg gtgatcaccg tgccggccta cttcaacgac tcgcagcgcc aggccaccaa 120
<210> 430
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 430
gaaggagatc gccgaggcgt acctgggcta cccggtgacc aacgcggtga tcaccgtgcc 60
ggcctacttc aacgactcgc agcgccaggc caccaaggat gcgggtgtga tcgcggggct 120
<210> 431
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 431
gggctacccg gtgaccaacg cggtgatcac cgtgccggcc tacttcaacg actcgcagcg 60
ccaggccacc aaggatgcgg gtgtgatcgc ggggctcaac gtgctgcgga tcatcaacga 120
<210> 432
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 432
gatcaccgtg ccggcctact tcaacgactc gcagcgccag gccaccaagg atgcgggtgt 60
gatcgcgggg ctcaacgtgc tgcggatcat caacgagccc acggccgccg ccatcgccta 120
<210> 433
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 433
cgactcgcag cgccaggcca ccaaggatgc gggtgtgatc gcggggctca acgtgctgcg 60
gatcatcaac gagcccacgg ccgccgccat cgcctacggc ctggacagaa cgggcaaggg 120
<210> 434
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 434
ggatgcgggt gtgatcgcgg ggctcaacgt gctgcggatc atcaacgagc ccacggccgc 60
cgccatcgcc tacggcctgg acagaacggg caagggggag cgcaacgtgc tcatctttga 120
<210> 435
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 435
caacgtgctg cggatcatca acgagcccac ggccgccgcc atcgcctacg gcctggacag 60
aacgggcaag ggggagcgca acgtgctcat ctttgacctg ggcgggggca ccttcgacgt 120
<210> 436
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 436
gcccacggcc gccgccatcg cctacggcct ggacagaacg ggcaaggggg agcgcaacgt 60
gctcatcttt gacctgggcg ggggcacctt cgacgtgtcc atcctgacga tcgacgacgg 120
<210> 437
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 437
cggcctggac agaacgggca agggggagcg caacgtgctc atctttgacc tgggcggggg 60
caccttcgac gtgtccatcc tgacgatcga cgacggcatc ttcgaggtga aggccacggc 120
<210> 438
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 438
ggagcgcaac gtgctcatct ttgacctggg cgggggcacc ttcgacgtgt ccatcctgac 60
gatcgacgac ggcatcttcg aggtgaaggc cacggccggg gacacccacc tgggtgggga 120
<210> 439
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 439
cctgggcggg ggcaccttcg acgtgtccat cctgacgatc gacgacggca tcttcgaggt 60
gaaggccacg gccggggaca cccacctggg tggggaggac tttgacaaca ggctggtgaa 120
<210> 440
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 440
gtccatcctg acgatcgacg acggcatctt cgaggtgaag gccacggccg gggacaccca 60
cctgggtggg gaggactttg acaacaggct ggtgaaccac ttcgtggagg agttcaagag 120
<210> 441
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 441
catcttcgag gtgaaggcca cggccgggga cacccacctg ggtggggagg actttgacaa 60
caggctggtg aaccacttcg tggaggagtt caagagaaaa cacaagaagg acatcagcca 120
<210> 442
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 442
cggggacacc cacctgggtg gggaggactt tgacaacagg ctggtgaacc acttcgtgga 60
ggagttcaag agaaaacaca agaaggacat cagccagaac aagcgagccg tgaggcggct 120
<210> 443
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 443
ggactttgac aacaggctgg tgaaccactt cgtggaggag ttcaagagaa aacacaagaa 60
ggacatcagc cagaacaagc gagccgtgag gcggctgcgc accgcctgcg agagggccaa 120
<210> 444
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 444
ccacttcgtg gaggagttca agagaaaaca caagaaggac atcagccaga acaagcgagc 60
cgtgaggcgg ctgcgcaccg cctgcgagag ggccaagagg accctgtcgt ccagcaccca 120
<210> 445
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 445
aaaacacaag aaggacatca gccagaacaa gcgagccgtg aggcggctgc gcaccgcctg 60
cgagagggcc aagaggaccc tgtcgtccag cacccaggcc agcctggaga tcgactccct 120
<210> 446
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 446
gaacaagcga gccgtgaggc ggctgcgcac cgcctgcgag agggccaaga ggaccctgtc 60
gtccagcacc caggccagcc tggagatcga ctccctgttt gagggcatcg acttctacac 120
<210> 447
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 447
gcgcaccgcc tgcgagaggg ccaagaggac cctgtcgtcc agcacccagg ccagcctgga 60
gatcgactcc ctgtttgagg gcatcgactt ctacacgtcc atcaccaggg cgaggttcga 120
<210> 448
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 448
gaggaccctg tcgtccagca cccaggccag cctggagatc gactccctgt ttgagggcat 60
cgacttctac acgtccatca ccagggcgag gttcgaggag ctgtgctccg acctgttccg 120
<210> 449
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 449
ggccagcctg gagatcgact ccctgtttga gggcatcgac ttctacacgt ccatcaccag 60
ggcgaggttc gaggagctgt gctccgacct gttccgaagc accctggagc ccgtggagaa 120
<210> 450
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 450
gtttgagggc atcgacttct acacgtccat caccagggcg aggttcgagg agctgtgctc 60
cgacctgttc cgaagcaccc tggagcccgt ggagaaggct ctgcgcgacg ccaagctgga 120
<210> 451
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 451
gtccatcacc agggcgaggt tcgaggagct gtgctccgac ctgttccgaa gcaccctgga 60
gcccgtggag aaggctctgc gcgacgccaa gctggacaag gcccagattc acgacctggt 120
<210> 452
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 452
ggagctgtgc tccgacctgt tccgaagcac cctggagccc gtggagaagg ctctgcgcga 60
cgccaagctg gacaaggccc agattcacga cctggtcctg gtcgggggct ccacccgcat 120
<210> 453
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 453
aagcaccctg gagcccgtgg agaaggctct gcgcgacgcc aagctggaca aggcccagat 60
tcacgacctg gtcctggtcg ggggctccac ccgcatcccc aaggtgcaga agctgctgca 120
<210> 454
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 454
ggctctgcgc gacgccaagc tggacaaggc ccagattcac gacctggtcc tggtcggggg 60
ctccacccgc atccccaagg tgcagaagct gctgcaggac ttcttcaacg ggcgcgacct 120
<210> 455
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 455
caaggcccag attcacgacc tggtcctggt cgggggctcc acccgcatcc ccaaggtgca 60
gaagctgctg caggacttct tcaacgggcg cgacctgaac aagagcatca accccgacga 120
<210> 456
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 456
cctggtcggg ggctccaccc gcatccccaa ggtgcagaag ctgctgcagg acttcttcaa 60
cgggcgcgac ctgaacaaga gcatcaaccc cgacgaggct gtggcctacg gggcggcggt 120
<210> 457
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 457
ccccaaggtg cagaagctgc tgcaggactt cttcaacggg cgcgacctga acaagagcat 60
caaccccgac gaggctgtgg cctacggggc ggcggtgcag gcggccatcc tgatggggga 120
<210> 458
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 458
ggacttcttc aacgggcgcg acctgaacaa gagcatcaac cccgacgagg ctgtggccta 60
cggggcggcg gtgcaggcgg ccatcctgat gggggacaag tccgagaacg tgcaggacct 120
<210> 459
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 459
gaacaagagc atcaaccccg acgaggctgt ggcctacggg gcggcggtgc aggcggccat 60
cctgatgggg gacaagtccg agaacgtgca ggacctgctg ctgctggacg tggctcccct 120
<210> 460
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 460
ggctgtggcc tacggggcgg cggtgcaggc ggccatcctg atgggggaca agtccgagaa 60
cgtgcaggac ctgctgctgc tggacgtggc tcccctgtcg ctggggctgg agacggccgg 120
<210> 461
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 461
gcaggcggcc atcctgatgg gggacaagtc cgagaacgtg caggacctgc tgctgctgga 60
cgtggctccc ctgtcgctgg ggctggagac ggccggaggc gtgatgactg ccctgatcaa 120
<210> 462
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 462
caagtccgag aacgtgcagg acctgctgct gctggacgtg gctcccctgt cgctggggct 60
ggagacggcc ggaggcgtga tgactgccct gatcaagcgc aactccacca tccccaccaa 120
<210> 463
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 463
gctgctgctg gacgtggctc ccctgtcgct ggggctggag acggccggag gcgtgatgac 60
tgccctgatc aagcgcaact ccaccatccc caccaagcag acgcagatct tcaccaccta 120
<210> 464
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 464
gtcgctgggg ctggagacgg ccggaggcgt gatgactgcc ctgatcaagc gcaactccac 60
catccccacc aagcagacgc agatcttcac cacctactcc gacaaccaac ccggggtgct 120
<210> 465
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 465
aggcgtgatg actgccctga tcaagcgcaa ctccaccatc cccaccaagc agacgcagat 60
cttcaccacc tactccgaca accaacccgg ggtgctgatc caggtgtacg agggcgagag 120
<210> 466
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 466
gcgcaactcc accatcccca ccaagcagac gcagatcttc accacctact ccgacaacca 60
acccggggtg ctgatccagg tgtacgaggg cgagagggcc atgacgaaag acaacaatct 120
<210> 467
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 467
gcagacgcag atcttcacca cctactccga caaccaaccc ggggtgctga tccaggtgta 60
cgagggcgag agggccatga cgaaagacaa caatctgttg gggcgcttcg agctgagcgg 120
<210> 468
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 468
ctccgacaac caacccgggg tgctgatcca ggtgtacgag ggcgagaggg ccatgacgaa 60
agacaacaat ctgttggggc gcttcgagct gagcggcatc cctccggccc ccaggggcgt 120
<210> 469
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 469
gatccaggtg tacgagggcg agagggccat gacgaaagac aacaatctgt tggggcgctt 60
cgagctgagc ggcatccctc cggcccccag gggcgtgccc cagatcgagg tgaccttcga 120
<210> 470
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 470
ggccatgacg aaagacaaca atctgttggg gcgcttcgag ctgagcggca tccctccggc 60
ccccaggggc gtgccccaga tcgaggtgac cttcgacatc gatgccaacg gcatcctgaa 120
<210> 471
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 471
gttggggcgc ttcgagctga gcggcatccc tccggccccc aggggcgtgc cccagatcga 60
ggtgaccttc gacatcgatg ccaacggcat cctgaacgtc acggccacgg acaagagcac 120
<210> 472
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 472
catccctccg gcccccaggg gcgtgcccca gatcgaggtg accttcgaca tcgatgccaa 60
cggcatcctg aacgtcacgg ccacggacaa gagcaccggc aaggccaaca agatcaccat 120
<210> 473
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 473
gccccagatc gaggtgacct tcgacatcga tgccaacggc atcctgaacg tcacggccac 60
ggacaagagc accggcaagg ccaacaagat caccatcacc aacgacaagg gccgcctgag 120
<210> 474
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 474
catcgatgcc aacggcatcc tgaacgtcac ggccacggac aagagcaccg gcaaggccaa 60
caagatcacc atcaccaacg acaagggccg cctgagcaag gaggagatcg agcgcatggt 120
<210> 475
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 475
cgtcacggcc acggacaaga gcaccggcaa ggccaacaag atcaccatca ccaacgacaa 60
gggccgcctg agcaaggagg agatcgagcg catggtgcag gaggcggaga agtacaaagc 120
<210> 476
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 476
cggcaaggcc aacaagatca ccatcaccaa cgacaagggc cgcctgagca aggaggagat 60
cgagcgcatg gtgcaggagg cggagaagta caaagcggag gacgaggtgc agcgcgagag 120
<210> 477
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 477
caccaacgac aagggccgcc tgagcaagga ggagatcgag cgcatggtgc aggaggcgga 60
gaagtacaaa gcggaggacg aggtgcagcg cgagagggtg tcagccaaga acgccctgga 120
<210> 478
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 478
caaggaggag atcgagcgca tggtgcagga ggcggagaag tacaaagcgg aggacgaggt 60
gcagcgcgag agggtgtcag ccaagaacgc cctggagtcc tacgccttca acatgaagag 120
<210> 479
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 479
gcaggaggcg gagaagtaca aagcggagga cgaggtgcag cgcgagaggg tgtcagccaa 60
gaacgccctg gagtcctacg ccttcaacat gaagagcgcc gtggaggatg aggggctcaa 120
<210> 480
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 480
ggaggacgag gtgcagcgcg agagggtgtc agccaagaac gccctggagt cctacgcctt 60
caacatgaag agcgccgtgg aggatgaggg gctcaagggc aagatcagcg aggcggacaa 120
<210> 481
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 481
ggtgtcagcc aagaacgccc tggagtccta cgccttcaac atgaagagcg ccgtggagga 60
tgaggggctc aagggcaaga tcagcgaggc ggacaagaag aaggtgctgg acaagtgtca 120
<210> 482
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 482
gtcctacgcc ttcaacatga agagcgccgt ggaggatgag gggctcaagg gcaagatcag 60
cgaggcggac aagaagaagg tgctggacaa gtgtcaagag gtcatctcgt ggctggacgc 120
<210> 483
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 483
cgccgtggag gatgaggggc tcaagggcaa gatcagcgag gcggacaaga agaaggtgct 60
ggacaagtgt caagaggtca tctcgtggct ggacgccaac accttggccg agaaggacga 120
<210> 484
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 484
gggcaagatc agcgaggcgg acaagaagaa ggtgctggac aagtgtcaag aggtcatctc 60
gtggctggac gccaacacct tggccgagaa ggacgagttt gagcacaaga ggaaggagct 120
<210> 485
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 485
gaagaaggtg ctggacaagt gtcaagaggt catctcgtgg ctggacgcca acaccttggc 60
cgagaaggac gagtttgagc acaagaggaa ggagctggag caggtgtgta accccatcat 120
<210> 486
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 486
agaggtcatc tcgtggctgg acgccaacac cttggccgag aaggacgagt ttgagcacaa 60
gaggaaggag ctggagcagg tgtgtaaccc catcatcagc ggactgtacc agggtgccgg 120
<210> 487
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 487
caacaccttg gccgagaagg acgagtttga gcacaagagg aaggagctgg agcaggtgtg 60
taaccccatc atcagcggac tgtaccaggg tgccggtggt cccgggcctg ggggcttcgg 120
<210> 488
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 488
gtttgagcac aagaggaagg agctggagca ggtgtgtaac cccatcatca gcggactgta 60
ccagggtgcc ggtggtcccg ggcctggggg cttcggggct cagggtccca agggagggtc 120
<210> 489
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 489
ggagcaggtg tgtaacccca tcatcagcgg actgtaccag ggtgccggtg gtcccgggcc 60
tgggggcttc ggggctcagg gtcccaaggg agggtctggg tcaggcccca ccattgagga 120
<210> 490
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 490
gacagacaga aaatcttact cgggactgtg aggtcctact tctacacacc gtccaggagt 60
gaaccaggaa ttgagaaagt aggaaggagg tgtcccagac cccaagctag gaatggggag 120
<210> 491
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 491
actgtgaggt cctacttcta cacaccgtcc aggagtgaac caggaattga gaaagtagga 60
aggaggtgtc ccagacccca agctaggaat ggggagggaa atggaggaat cccaaatgcc 120
<210> 492
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 492
gctacccgaa ggtctccggg gatccgcaaa gccttcttcc aagacataac tgagacgaag 60
ggaaaagcag tggtagtcaa cacagctcgg acctgatgga tctgatgtac ctggcagaaa 120
<210> 493
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 493
cgcaaagcct tcttccaaga cataactgag acgaagggaa aagcagtggt agtcaacaca 60
gctcggacct gatggatctg atgtacctgg cagaaagaat aaaaacctgt ggatgtttcc 120
<210> 494
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 494
aagaatctta ccttatttca tagtgatgca gttgaaacat ggtagcctgc acagggtggt 60
agagctggcc cgttttgaaa tgagaagttg aattcctgat ctatggcttt ggcacttgct 120
<210> 495
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 495
gatgcagttg aaacatggta gcctgcacag ggtggtagag ctggcccgtt ttgaaatgag 60
aagttgaatt cctgatctat ggctttggca cttgctttgc caccaagaat tgctatctcc 120
<210> 496
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 496
gcacagggtg gtagagctgg cccgttttga aatgagaagt tgaattcctg atctatggct 60
ttggcacttg ctttgccacc aagaattgct atctccaaaa tctttttttt tttttttttt 120
<210> 497
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 497
ttttgaaatg agaagttgaa ttcctgatct atggctttgg cacttgcttt gccaccaaga 60
attgctatct ccaaaatctt tttttttttt tttttttttt ttttttttga gacagagtct 120
<210> 498
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 498
tgatctatgg ctttggcact tgctttgcca ccaagaattg ctatctccaa aatctttttt 60
tttttttttt tttttttttt ttttgagaca gagtctcgct ctgttgccca ggccggactg 120
<210> 499
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 499
tctcacctct accagggtct ctgttgctca caagagctca gggctgggag aagcctctgt 60
gtgacagatg aaggggtcct gctgctctct agggaagaat cggtcccaaa ttgctcaagg 120
<210> 500
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 500
tgctcacaag agctcagggc tgggagaagc ctctgtgtga cagatgaagg ggtcctgctg 60
ctctctaggg aagaatcggt cccaaattgc tcaagggagt aaggtggttt cgttgaggag 120
<210> 501
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 501
ggagtgaaga tctgacctta gtgcagagtt cagctgtgtt tgcccctcag atcatcttta 60
ggatggaatg attttcatgg ttcattaatg aatagtggag aagcaagtgg gaacttatca 120
<210> 502
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 502
agagttcagc tgtgtttgcc cctcagatca tctttaggat ggaatgattt tcatggttca 60
ttaatgaata gtggagaagc aagtgggaac ttatcaaaat ctgtattcta gaatgtacca 120
<210> 503
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 503
tggttgtggc atacacacgt gtgggtataa aatcgtaaat cctatgtagt cgcgtagtgc 60
atgcgcagaa ggcttagaca cgaaatgtca tttcagcaat gtgcctagag aagctctgac 120
<210> 504
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 504
gtataaaatc gtaaatccta tgtagtcgcg tagtgcatgc gcagaaggct tagacacgaa 60
atgtcatttc agcaatgtgc ctagagaagc tctgacgccg ccttggaagt aagtcgttgc 120
<210> 505
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 505
gctgcccata ttttggaaat acattcaatt tatctggtta tcgcatgtgt aaggttttct 60
gtaggtaaat acttttgctg ttaataacct gttttacaat tttatagtat ttttccactg 120
<210> 506
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 506
tcaatttatc tggttatcgc atgtgtaagg ttttctgtag gtaaatactt ttgctgttaa 60
taacctgttt tacaatttta tagtattttt ccactgaagc agtggttttc atttttttat 120
<210> 507
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 507
agtacctgcc actgtattat gtgaatttaa atgactgctc ttattacaag taggcatagt 60
gatagtattg acaagtgaca gtctaatgac aaaaagacaa aaatagcgaa gcataaatgt 120
<210> 508
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 508
atttaaatga ctgctcttat tacaagtagg catagtgata gtattgacaa gtgacagtct 60
aatgacaaaa agacaaaaat agcgaagcat aaatgtaaga atgcaaaatg cttagtcaca 120
<210> 509
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 509
ctttcccctc tctaattcag cctccacatg gtcatcacac acttttctga agtgtaattt 60
tgaccatgct gctttgttgc ttctacctct ccatttgctc ctcattgcct gtaggctaaa 120
<210> 510
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 510
cacatggtca tcacacactt ttctgaagtg taattttgac catgctgctt tgttgcttct 60
acctctccat ttgctcctca ttgcctgtag gctaaaattc agacttcgtg ggatagcact 120
<210> 511
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 511
gaagtgtaat tttgaccatg ctgctttgtt gcttctacct ctccatttgc tcctcattgc 60
ctgtaggcta aaattcagac ttcgtgggat agcactgaag atgctctatg cttttcacgc 120
<210> 512
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 512
tttgttgctt ctacctctcc atttgctcct cattgcctgt aggctaaaat tcagacttcg 60
tgggatagca ctgaagatgc tctatgcttt tcacgctttc tccctgacct ctatcatgca 120
<210> 513
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 513
gctcctcatt gcctgtaggc taaaattcag acttcgtggg atagcactga agatgctcta 60
tgcttttcac gctttctccc tgacctctat catgcagaaa tatttaactg cttgcaggaa 120
<210> 514
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 514
cactcatgct ggcctcaggg acagggatga ggtcactggc acatttcaag aacagcttcc 60
tttctttatg catacaccac tttcctggtc cttgttaagg gtctccttcc tcttgcggtt 120
<210> 515
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 515
ggatgaggtc actggcacat ttcaagaaca gcttcctttc tttatgcata caccactttc 60
ctggtccttg ttaagggtct ccttcctctt gcggttccca tctctgcagc tgaccccagt 120
<210> 516
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 516
aaatgtaaat tatgtgggtt acctttcgag gatgaagcct gaattgcaac atatgaatat 60
gaactttgag ttatgataat aaaacagact ttctctaaaa gtaattaatc gacattaatg 120
<210> 517
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 517
ttcgaggatg aagcctgaat tgcaacatat gaatatgaac tttgagttat gataataaaa 60
cagactttct ctaaaagtaa ttaatcgaca ttaatgttat acttaacttc taaagacaat 120
<210> 518
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 518
agagactgca taagcccttc acctttgtaa gttgtcagta gctctagccc gtgaacattt 60
cctacattcc tgggatttgt agaatcagtg ataggtagtt taatatcttt gtgtgatcag 120
<210> 519
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 519
ttgtaagttg tcagtagctc tagcccgtga acatttccta cattcctggg atttgtagaa 60
tcagtgatag gtagtttaat atctttgtgt gatcagtgtg tatttaaaat ccattgtgtg 120
<210> 520
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 520
cagcagtaaa tgagctcttt gcccaccttt ggctttcatt tgacaagcag gacaaatatc 60
aagcaagtaa ttacaatagt gagaaaggga aaatggtagg tcatatgggg gatattaaca 120
<210> 521
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 521
acctttggct ttcatttgac aagcaggaca aatatcaagc aagtaattac aatagtgaga 60
aagggaaaat ggtaggtcat atgggggata ttaacaagta aactaatttg gacataaagt 120
<210> 522
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 522
aggacaaata tcaagcaagt aattacaata gtgagaaagg gaaaatggta ggtcatatgg 60
gggatattaa caagtaaact aatttggaca taaagtaaaa atgaaactga tattttaagc 120
<210> 523
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 523
acaatagtga gaaagggaaa atggtaggtc atatggggga tattaacaag taaactaatt 60
tggacataaa gtaaaaatga aactgatatt ttaagcaaca gccaagatat gtttatatca 120
<210> 524
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 524
taggtcatat gggggatatt aacaagtaaa ctaatttgga cataaagtaa aaatgaaact 60
gatattttaa gcaacagcca agatatgttt atatcatgaa ttgatgaaat gataacattc 120
<210> 525
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 525
agtaaactaa tttggacata aagtaaaaat gaaactgata ttttaagcaa cagccaagat 60
atgtttatat catgaattga tgaaatgata acattcatga tttttagttc cctgttcaga 120
<210> 526
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 526
cacaacatgc ccttctggag gatatccagt cactcagacc tcctggctct ccaccaagca 60
ctggaattag agtatttgta actgtgttct ttagccggag atccattttt tatatttcaa 120
<210> 527
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 527
tccagtcact cagacctcct ggctctccac caagcactgg aattagagta tttgtaactg 60
tgttctttag ccggagatcc attttttata tttcaagtac actgaatttt tatgtgtgat 120
<210> 528
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 528
ctccaccaag cactggaatt agagtatttg taactgtgtt ctttagccgg agatccattt 60
tttatatttc aagtacactg aatttttatg tgtgattcaa tgcctctggc tctacacata 120
<210> 529
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 529
tatttgtaac tgtgttcttt agccggagat ccatttttta tatttcaagt acactgaatt 60
tttatgtgtg attcaatgcc tctggctcta cacatataaa ttgtcttaat ggatgaaatc 120
<210> 530
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 530
tttcccatgg aaactcagtg aaaatgacag aagattatag gatttttaaa gtttttgaca 60
tgcaagcata atgcagacct ctttgatggt tgacagattc ttttattaac agtcaaaaac 120
<210> 531
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 531
tgacagaaga ttataggatt tttaaagttt ttgacatgca agcataatgc agacctcttt 60
gatggttgac agattctttt attaacagtc aaaaacttca cacaattgga aaataaatgt 120
<210> 532
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 532
aaaaaccact gggtgacatc taccagaagt atcactgcag aggattactc gacaaaaagt 60
gcaattttct atagaaagcc gagtgtttcc cttgggaaag tgttaatatt ttatttttaa 120
<210> 533
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 533
agaagtatca ctgcagagga ttactcgaca aaaagtgcaa ttttctatag aaagccgagt 60
gtttcccttg ggaaagtgtt aatattttat ttttaagtaa ctggggcaaa agactagatt 120
<210> 534
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 534
tggctctatt tcccatactc ttcagtgaca ttttttaaat ccaattaggc tcaacagcaa 60
agtatttggt gagcccagcc tggcacttcc aagccgaata ttttcatata ctctaccaaa 120
<210> 535
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 535
gtgacatttt ttaaatccaa ttaggctcaa cagcaaagta tttggtgagc ccagcctggc 60
acttccaagc cgaatatttt catatactct accaaagagc cccagaaagg cagaaggtct 120
<210> 536
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 536
aaaaatccaa aaccacccac aaatgaacag atttcaataa ttacagtaaa atgttatatt 60
acatgtatta aaatattttt gtcatagggc ccaagaaatt agacaacatc agaatgcgac 120
<210> 537
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 537
gaacagattt caataattac agtaaaatgt tatattacat gtattaaaat atttttgtca 60
tagggcccaa gaaattagac aacatcagaa tgcgacacct ggatctaagt ttcacaattt 120
<210> 538
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 538
aaaaaacatg actatcgatt atcttccttg tgcaaaccac taacgaataa attaaaaaga 60
caatactatt ttgtaaaaaa cgttaaaaca taacattccc atacacaccc ccaaaaggca 120
<210> 539
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 539
tccttgtgca aaccactaac gaataaatta aaaagacaat actattttgt aaaaaacgtt 60
aaaacataac attcccatac acacccccaa aaggcacaga ctcaaaaagt ccacataccc 120
<210> 540
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 540
gctagtgctg gtgctaccgc tgatgccgcc gatctgctga agccgctgcc gaagccacca 60
cagccacgag tgccgctcct gcgccgcccg cgggccttaa gaaagcgcgg ggagcagggc 120
<210> 541
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 541
gccgccgatc tgctgaagcc gctgccgaag ccaccacagc cacgagtgcc gctcctgcgc 60
cgcccgcggg ccttaagaaa gcgcggggag cagggccgcg accccagctg cgccgcaagg 120
<210> 542
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 542
ccgaagccac cacagccacg agtgccgctc ctgcgccgcc cgcgggcctt aagaaagcgc 60
ggggagcagg gccgcgaccc cagctgcgcc gcaagggcac cgccgccccg cccccccccc 120
<210> 543
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 543
ccgctcctgc gccgcccgcg ggccttaaga aagcgcgggg agcagggccg cgaccccagc 60
tgcgccgcaa gggcaccgcc gccccgcccc ccccccccgc gggccccgcc ccgcgcccgc 120
<210> 544
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 544
ttaagaaagc gcggggagca gggccgcgac cccagctgcg ccgcaagggc accgccgccc 60
cgcccccccc ccccgcgggc cccgccccgc gcccgccgcg cccaaccccg ggggtgccct 120
<210> 545
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 545
cgcgacccca gctgcgccgc aagggcaccg ccgccccgcc cccccccccc gcgggccccg 60
ccccgcgccc gccgcgccca accccggggg tgccctgcgt gcctgtcccg cccgcccgcg 120
<210> 546
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 546
gcaccgccgc cccgcccccc ccccccgcgg gccccgcccc gcgcccgccg cgcccaaccc 60
cgggggtgcc ctgcgtgcct gtcccgcccg cccgcgcttg ccgtacaccc cgcgcccagg 120
<210> 547
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 547
ccgcgggccc cgccccgcgc ccgccgcgcc caaccccggg ggtgccctgc gtgcctgtcc 60
cgcccgcccg cgcttgccgt acaccccgcg cccagggcac gggagaaagg aggctgcagg 120
<210> 548
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 548
cgcgcccaac cccgggggtg ccctgcgtgc ctgtcccgcc cgcccgcgct tgccgtacac 60
cccgcgccca gggcacggga gaaaggaggc tgcaggctag cctcggcctc ggcaacctcg 120
<210> 549
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 549
gcgtgcctgt cccgcccgcc cgcgcttgcc gtacaccccg cgcccagggc acgggagaaa 60
ggaggctgca ggctagcctc ggcctcggca acctcgctgc ctgtctgccg tacttgagtg 120
<210> 550
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 550
tggaaatcac agccctccta cccgccctaa acagaatcca ctgaacactg gacactgctc 60
ctagccagtg tgaaattgtt tgaaaagaaa aagctcagcc tagactactc aagactccac 120
<210> 551
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 551
ccctaaacag aatccactga acactggaca ctgctcctag ccagtgtgaa attgtttgaa 60
aagaaaaagc tcagcctaga ctactcaaga ctccactgct taaaatgccc acctccctcc 120
<210> 552
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 552
gatgataggg aaaaagtatg agaagtgaag taaagtggtt tgataaatta caatggaaag 60
aaacacatga aagtctctct ctagggctta tgatgatttt ataggaggta cagtgagcag 120
<210> 553
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 553
gtgaagtaaa gtggtttgat aaattacaat ggaaagaaac acatgaaagt ctctctctag 60
ggcttatgat gattttatag gaggtacagt gagcagaaaa actctccctc tgtgagctgg 120
<210> 554
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 554
tacaatggaa agaaacacat gaaagtctct ctctagggct tatgatgatt ttataggagg 60
tacagtgagc agaaaaactc tccctctgtg agctgggcac gcggccctga tcctagcatt 120
<210> 555
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 555
gtctctctct agggcttatg atgattttat aggaggtaca gtgagcagaa aaactctccc 60
tctgtgagct gggcacgcgg ccctgatcct agcattgctg ctgcttaacc atgtggtcac 120
<210> 556
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 556
ttttatagga ggtacagtga gcagaaaaac tctccctctg tgagctgggc acgcggccct 60
gatcctagca ttgctgctgc ttaaccatgt ggtcacggac gactgacttc attctcctgg 120
<210> 557
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 557
aaaaactctc cctctgtgag ctgggcacgc ggccctgatc ctagcattgc tgctgcttaa 60
ccatgtggtc acggacgact gacttcattc tcctggcttt agttcacagt tctgttcaat 120
<210> 558
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 558
gcacgcggcc ctgatcctag cattgctgct gcttaaccat gtggtcacgg acgactgact 60
tcattctcct ggctttagtt cacagttctg ttcaatgagg aggtggcaag accttcctgt 120
<210> 559
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 559
gctgctgctt aaccatgtgg tcacggacga ctgacttcat tctcctggct ttagttcaca 60
gttctgttca atgaggaggt ggcaagacct tcctgtccca acccttattt cctctttgtc 120
<210> 560
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 560
ggacgactga cttcattctc ctggctttag ttcacagttc tgttcaatga ggaggtggca 60
agaccttcct gtcccaaccc ttatttcctc tttgtctcta aagtggactc ccattaagga 120
<210> 561
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 561
ctttagttca cagttctgtt caatgaggag gtggcaagac cttcctgtcc caacccttat 60
ttcctctttg tctctaaagt ggactcccat taaggaagag agctgatgga aacctcctta 120
<210> 562
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 562
gaggaggtgg caagaccttc ctgtcccaac ccttatttcc tctttgtctc taaagtggac 60
tcccattaag gaagagagct gatggaaacc tccttaggaa ctgggaattt gagttgcaat 120
<210> 563
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 563
cccaaccctt atttcctctt tgtctctaaa gtggactccc attaaggaag agagctgatg 60
gaaacctcct taggaactgg gaatttgagt tgcaatattt cattgatttt tgttgttgtt 120
<210> 564
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 564
taaggaagag agctgatgga aacctcctta ggaactggga atttgagttg caatatttca 60
ttgatttttg ttgttgttgt ttcaaattaa gagtccattc actttcagct ttccacaaga 120
<210> 565
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 565
tccttaggaa ctgggaattt gagttgcaat atttcattga tttttgttgt tgttgtttca 60
aattaagagt ccattcactt tcagctttcc acaagagaaa ttaatatggc catgcttgcc 120
<210> 566
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 566
taatgtttat aaatgcttac aataaaatgc ataaattttc tctgtgcggt tcatgaaaaa 60
aagaaagaag aaaaaaaaat tttaaataaa tgaataaaat gtatagattg caaagaaaat 120
<210> 567
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 567
aaatgcataa attttctctg tgcggttcat gaaaaaaaga aagaagaaaa aaaaatttta 60
aataaatgaa taaaatgtat agattgcaaa gaaaatcaat tacattgtaa ggaaaaaaaa 120
<210> 568
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 568
gttcatgaaa aaaagaaaga agaaaaaaaa attttaaata aatgaataaa atgtatagat 60
tgcaaagaaa atcaattaca ttgtaaggaa aaaaaatagc tgagagcagt ctgagctttg 120
<210> 569
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 569
aaaaaaattt taaataaatg aataaaatgt atagattgca aagaaaatca attacattgt 60
aaggaaaaaa aatagctgag agcagtctga gctttgtgag gtatgcaaaa tttatcaggc 120
<210> 570
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 570
aaatgtatag attgcaaaga aaatcaatta cattgtaagg aaaaaaaata gctgagagca 60
gtctgagctt tgtgaggtat gcaaaattta tcaggcccag agcgacatga gtatgggact 120
<210> 571
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 571
caattacatt gtaaggaaaa aaaatagctg agagcagtct gagctttgtg aggtatgcaa 60
aatttatcag gcccagagcg acatgagtat gggacttcag tcacgcccca atacccatgc 120
<210> 572
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 572
tagctgagag cagtctgagc tttgtgaggt atgcaaaatt tatcaggccc agagcgacat 60
gagtatggga cttcagtcac gccccaatac ccatgcctgg ggacagttgt ttaaagacat 120
<210> 573
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 573
tgaggtatgc aaaatttatc aggcccagag cgacatgagt atgggacttc agtcacgccc 60
caatacccat gcctggggac agttgtttaa agacattttg ttcccgagta gctgtcccac 120
<210> 574
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 574
ccagagcgac atgagtatgg gacttcagtc acgccccaat acccatgcct ggggacagtt 60
gtttaaagac attttgttcc cgagtagctg tcccacccat tatcttcata ttcctggaat 120
<210> 575
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 575
tcagtcacgc cccaataccc atgcctgggg acagttgttt aaagacattt tgttcccgag 60
tagctgtccc acccattatc ttcatattcc tggaatttat gatacaaaga acaaagtata 120
<210> 576
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 576
ctggggacag ttgtttaaag acattttgtt cccgagtagc tgtcccaccc attatcttca 60
tattcctgga atttatgata caaagaacaa agtataacca atccacagct tatgttattt 120
<210> 577
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 577
tttgttcccg agtagctgtc ccacccatta tcttcatatt cctggaattt atgatacaaa 60
gaacaaagta taaccaatcc acagcttatg ttattttaac gcaaggaatt taggaactgc 120
<210> 578
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 578
ccattatctt catattcctg gaatttatga tacaaagaac aaagtataac caatccacag 60
cttatgttat tttaacgcaa ggaatttagg aactgcctct tccttcccct taaaaatcta 120
<210> 579
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 579
ttatgataca aagaacaaag tataaccaat ccacagctta tgttatttta acgcaaggaa 60
tttaggaact gcctcttcct tccccttaaa aatctatgtg taactgctgc taatcagagt 120
<210> 580
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 580
actctttggt agcagcggtt ataaattacg cctttgctaa tttgcgttgt tcccaaccag 60
gagaaacatt accacaaaaa aagtcagttt catcctgcag tgttcccgca gcaaccatat 120
<210> 581
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 581
attacgcctt tgctaatttg cgttgttccc aaccaggaga aacattacca caaaaaaagt 60
cagtttcatc ctgcagtgtt cccgcagcaa ccatattaag ctggaagaat aaagctcctt 120
<210> 582
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 582
gtttcagtga gaggcagctt aagtgttttc acatggaagc ctttctcccg tgaggcatgc 60
ccgcagtgca gaggttggat ggggggtgat ttccaggatc aaggagatag ggtgtccagg 120
<210> 583
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 583
gttttcacat ggaagccttt ctcccgtgag gcatgcccgc agtgcagagg ttggatgggg 60
ggtgatttcc aggatcaagg agatagggtg tccaggaaag cctggggatt gcacctaaga 120
<210> 584
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 584
aatcactgtt tttctctttc caaataattg aatcactttg gattgtttat atttttccag 60
aaatgttccc attttgtctg ttttcaaata ttagcataaa gtgattcaca aaattgtctt 120
<210> 585
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 585
taattgaatc actttggatt gtttatattt ttccagaaat gttcccattt tgtctgtttt 60
caaatattag cataaagtga ttcacaaaat tgtctttttt cagaagagaa ttgtcacatc 120
<210> 586
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 586
atatttttcc agaaatgttc ccattttgtc tgttttcaaa tattagcata aagtgattca 60
caaaattgtc ttttttcaga agagaattgt cacatctaaa attgtcttcc ttttctaata 120
<210> 587
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 587
tttgtctgtt ttcaaatatt agcataaagt gattcacaaa attgtctttt ttcagaagag 60
aattgtcaca tctaaaattg tcttcctttt ctaatatttg attatctttg ccagtttgtt 120
<210> 588
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 588
taaagtgatt cacaaaattg tcttttttca gaagagaatt gtcacatcta aaattgtctt 60
ccttttctaa tatttgatta tctttgccag tttgttagtt ttctttgcca tttcaaagaa 120
<210> 589
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 589
ttttcagaag agaattgtca catctaaaat tgtcttcctt ttctaatatt tgattatctt 60
tgccagtttg ttagttttct ttgccatttc aaagaaccaa ttttcatctt aaattgtcct 120
<210> 590
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 590
taaaattgtc ttccttttct aatatttgat tatctttgcc agtttgttag ttttctttgc 60
catttcaaag aaccaatttt catcttaaat tgtcctttct gtcaagtctt tacttcgtta 120
<210> 591
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 591
gtttcaccaa accactggct caaagagcct gactcagagg cctagcgacc ggaattccac 60
tctgctaaac gtgcatttcc catctgcccc accccaaaca tatcttacca atgtcagtgt 120
<210> 592
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 592
gagcctgact cagaggccta gcgaccggaa ttccactctg ctaaacgtgc atttcccatc 60
tgccccaccc caaacatatc ttaccaatgt cagtgtcttt ttttctgacc tgacacaaaa 120
<210> 593
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 593
taataaaaga ctagtggcct tagtgcccat gcccagtgac cctccattca atacccgaag 60
agcctacacc agtgaggatg aagcctggaa gtcatacttg gagaatcccc tgacagcagc 120
<210> 594
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 594
gcccatgccc agtgaccctc cattcaatac ccgaagagcc tacaccagtg aggatgaagc 60
ctggaagtca tacttggaga atcccctgac agcagccacc aaggccatga tgagcattaa 120
<210> 595
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 595
caatacccga agagcctaca ccagtgagga tgaagcctgg aagtcatact tggagaatcc 60
cctgacagca gccaccaagg ccatgatgag cattaatggt gatgaggaca gtgctgctgc 120
<210> 596
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 596
tgaggatgaa gcctggaagt catacttgga gaatcccctg acagcagcca ccaaggccat 60
gatgagcatt aatggtgatg aggacagtgc tgctgccctc ggcctgctct atgactacta 120
<210> 597
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 597
acacctattc ttctgccaga gttttctaaa attacacatt cctttcccac cttctttccc 60
attaaaacac tcaggaggga ttccttcttg gggagatgat tgaaaacaaa gcgaggcttt 120
<210> 598
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 598
tctaaaatta cacattcctt tcccaccttc tttcccatta aaacactcag gagggattcc 60
ttcttgggga gatgattgaa aacaaagcga ggctttatct aagatgaaga gcaaggtatg 120
<210> 599
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 599
agtgatcatt tgctttgctg acatacaact gcattttata cactctggcc aagatgtttt 60
tcaggtgttc cctgaaaatt atactttttt tttaacctaa agtttagtgt ttgatgaagc 120
<210> 600
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 600
acaactgcat tttatacact ctggccaaga tgtttttcag gtgttccctg aaaattatac 60
ttttttttta acctaaagtt tagtgtttga tgaagctgtt gttatcttta gagcaccatc 120
<210> 601
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 601
ccaagatgtt tttcaggtgt tccctgaaaa ttatactttt tttttaacct aaagtttagt 60
gtttgatgaa gctgttgtta tctttagagc accatcgtat attctctctc aatcttccct 120
<210> 602
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 602
tgaaaattat actttttttt taacctaaag tttagtgttt gatgaagctg ttgttatctt 60
tagagcacca tcgtatattc tctctcaatc ttccctctga cttctgtgat tgcaggaacc 120
<210> 603
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 603
ctaaagttta gtgtttgatg aagctgttgt tatctttaga gcaccatcgt atattctctc 60
tcaatcttcc ctctgacttc tgtgattgca ggaaccataa ccagtgcatc tatgctagtg 120
<210> 604
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 604
catcttggtg aacatggatg acaacatcat cgagcactac tcgaacgagg acaccttcat 60
cctcaacatg gagagcatgg tggagggctt caaggtcacg ctcatggaaa tctagccctg 120
<210> 605
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 605
catcatcgag cactactcga acgaggacac cttcatcctc aacatggaga gcatggtgga 60
gggcttcaag gtcacgctca tggaaatcta gccctgggtt tggcatccgc tttggctgga 120
<210> 606
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 606
ggacaccttc atcctcaaca tggagagcat ggtggagggc ttcaaggtca cgctcatgga 60
aatctagccc tgggtttggc atccgctttg gctggagctc tcagtgcgtt cctccctgag 120
<210> 607
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 607
gagcatggtg gagggcttca aggtcacgct catggaaatc tagccctggg tttggcatcc 60
gctttggctg gagctctcag tgcgttcctc cctgagagag acagaagccc cagccccaga 120
<210> 608
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 608
cacgctcatg gaaatctagc cctgggtttg gcatccgctt tggctggagc tctcagtgcg 60
ttcctccctg agagagacag aagccccagc cccagaacct ggagacccat ctcccccatc 120
<210> 609
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 609
ggtttggcat ccgctttggc tggagctctc agtgcgttcc tccctgagag agacagaagc 60
cccagcccca gaacctggag acccatctcc cccatctcac aactgctgtt acaagaccgt 120
<210> 610
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 610
ttggattcct atttattgcc caccttttcc tggagcccag gtccaggccc gccaggactc 60
tgcaggtcac tgctagctcc agatgagacc gtccagcgtt cccccttcaa gagaaacact 120
<210> 611
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 611
ttttcctgga gcccaggtcc aggcccgcca ggactctgca ggtcactgct agctccagat 60
gagaccgtcc agcgttcccc cttcaagaga aacactcatc ccgaacagcc taaaaaattc 120
<210> 612
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 612
ccgccaggac tctgcaggtc actgctagct ccagatgaga ccgtccagcg ttcccccttc 60
aagagaaaca ctcatcccga acagcctaaa aaattcccat cccttctctc tcacccctcc 120
<210> 613
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 613
ctagctccag atgagaccgt ccagcgttcc cccttcaaga gaaacactca tcccgaacag 60
cctaaaaaat tcccatccct tctctctcac ccctccatat ctatctcccg agtggctgga 120
<210> 614
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 614
cgttccccct tcaagagaaa cactcatccc gaacagccta aaaaattccc atcccttctc 60
tctcacccct ccatatctat ctcccgagtg gctggacaaa atgagctacg tctgggtgca 120
<210> 615
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 615
catcccgaac agcctaaaaa attcccatcc cttctctctc acccctccat atctatctcc 60
cgagtggctg gacaaaatga gctacgtctg ggtgcagtag ttataggtgg ggcaagaggt 120
<210> 616
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 616
tttatctcac aatgatgtgt tttgcttgac tttccctttg cgctgtctcg tgggaaaggt 60
cattctgtct gagaccccag ctccttctcc agctttggct gcgggcatgg cctgagcttt 120
<210> 617
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 617
cttgactttc cctttgcgct gtctcgtggg aaaggtcatt ctgtctgaga ccccagctcc 60
ttctccagct ttggctgcgg gcatggcctg agctttctgg agagcctctg cagggggttt 120
<210> 618
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 618
ggagcacccc agtttgcctt tgatgtgtcc gctgtgtatg ttagctgaac tttgatgagc 60
aaaatttcct gagcgaaaca ctccaaagag ataggaaaac ttgccgcctc ttcttttttg 120
<210> 619
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 619
gtgtccgctg tgtatgttag ctgaactttg atgagcaaaa tttcctgagc gaaacactcc 60
aaagagatag gaaaacttgc cgcctcttct tttttgtccc ttaatcaaac tcaaataagc 120
<210> 620
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 620
ctcccctgct ttctttataa gtcaggtcga gagtggtttt accaaacgcg tgggcatagg 60
gtggttaaag taaatgaggg agaaaccaga aaccgtcctg gtaacacggg aggtaattac 120
<210> 621
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 621
ggtcgagagt ggttttacca aacgcgtggg catagggtgg ttaaagtaaa tgagggagaa 60
accagaaacc gtcctggtaa cacgggaggt aattacgtca gaaaccaggg acgagactga 120
<210> 622
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 622
aagctgcgct ggagtcctga tgctgtcact tagtagcggg gcattccctt ccccgaacct 60
cagcttcctt atgagcaaac gagaactggc ccagcacatc tctcagctcc cttcaacact 120
<210> 623
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 623
gtcacttagt agcggggcat tcccttcccc gaacctcagc ttccttatga gcaaacgaga 60
actggcccag cacatctctc agctcccttc aacactctgt gcttctggga ttcctgaaag 120
<210> 624
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 624
ttccccgaac ctcagcttcc ttatgagcaa acgagaactg gcccagcaca tctctcagct 60
cccttcaaca ctctgtgctt ctgggattcc tgaaagattt ccccaagtaa cagtgcatta 120
<210> 625
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 625
gagcaaacga gaactggccc agcacatctc tcagctccct tcaacactct gtgcttctgg 60
gattcctgaa agatttcccc aagtaacagt gcattatctt cccccagcct cacaggggtt 120
<210> 626
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 626
catctctcag ctcccttcaa cactctgtgc ttctgggatt cctgaaagat ttccccaagt 60
aacagtgcat tatcttcccc cagcctcaca ggggtttcct tagagtcact gcccccacaa 120
<210> 627
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 627
ctgtgcttct gggattcctg aaagatttcc ccaagtaaca gtgcattatc ttcccccagc 60
ctcacagggg tttccttaga gtcactgccc ccacaaatag cctctcagag taaaagtttc 120
<210> 628
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 628
atttccccaa gtaacagtgc attatcttcc cccagcctca caggggtttc cttagagtca 60
ctgcccccac aaatagcctc tcagagtaaa agtttccccc atggcaagtt tgcctcctca 120
<210> 629
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 629
tcaggtgaga ggggtgagct gacactccac aggccatggc atgtgccatc ttgggtctgt 60
gcaatgcctt ctcttgatag atgaatggat acattgattc ccttctattt ccatccacca 120
<210> 630
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 630
ctccacaggc catggcatgt gccatcttgg gtctgtgcaa tgccttctct tgatagatga 60
atggatacat tgattccctt ctatttccat ccaccactcc aatctccacc cctataggtg 120
<210> 631
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 631
tcttgggtct gtgcaatgcc ttctcttgat agatgaatgg atacattgat tcccttctat 60
ttccatccac cactccaatc tccaccccta taggtgacca tcagaatgag cttctttaat 120
<210> 632
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 632
cttgatagat gaatggatac attgattccc ttctatttcc atccaccact ccaatctcca 60
cccctatagg tgaccatcag aatgagcttc tttaatatgc attgctgcat attgtggcgt 120
<210> 633
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 633
attcccttct atttccatcc accactccaa tctccacccc tataggtgac catcagaatg 60
agcttcttta atatgcattg ctgcatattg tggcgtatgg ggttgcatgt gtgcattttg 120
<210> 634
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 634
ctccaatctc cacccctata ggtgaccatc agaatgagct tctttaatat gcattgctgc 60
atattgtggc gtatggggtt gcatgtgtgc attttggttt acctaaatgg tatcaggtca 120
<210> 635
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 635
accatcagaa tgagcttctt taatatgcat tgctgcatat tgtggcgtat ggggttgcat 60
gtgtgcattt tggtttacct aaatggtatc aggtcatcca actcattcag tctccttctt 120
<210> 636
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 636
atgcattgct gcatattgtg gcgtatgggg ttgcatgtgt gcattttggt ttacctaaat 60
ggtatcaggt catccaactc attcagtctc cttctttttc ctcatgactg tgcttttgtg 120
<210> 637
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 637
atggggttgc atgtgtgcat tttggtttac ctaaatggta tcaggtcatc caactcattc 60
agtctccttc tttttcctca tgactgtgct tttgtggctc acttgcggtg ctgggtgtgt 120
<210> 638
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 638
gtttacctaa atggtatcag gtcatccaac tcattcagtc tccttctttt tcctcatgac 60
tgtgcttttg tggctcactt gcggtgctgg gtgtgtgcat tcccttgctt ccaatgtttg 120
<210> 639
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 639
tccaactcat tcagtctcct tctttttcct catgactgtg cttttgtggc tcacttgcgg 60
tgctgggtgt gtgcattccc ttgcttccaa tgtttgtata caacccatgg tgaacaccca 120
<210> 640
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 640
tttcctcatg actgtgcttt tgtggctcac ttgcggtgct gggtgtgtgc attcccttgc 60
ttccaatgtt tgtatacaac ccatggtgaa cacccacttc ttttctctgc cttctccccc 120
<210> 641
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 641
gctcacttgc ggtgctgggt gtgtgcattc ccttgcttcc aatgtttgta tacaacccat 60
ggtgaacacc cacttctttt ctctgccttc tcccccagag atggacactg ctgtggctgc 120
<210> 642
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 642
gcattccctt gcttccaatg tttgtataca acccatggtg aacacccact tcttttctct 60
gccttctccc ccagagatgg acactgctgt ggctgctgac tccataaaca agggggagac 120
<210> 643
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 643
gctcctccag cacagtcagt ggcttcctgg agccctcagc ctggctgtgt ttactgaacc 60
tcacaaacta gaagaggaag aaaaaaaaag agagagagaa acaaagagaa ataaccatgg 120
<210> 644
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 644
gcctgtggca ggccttccag gcacataccc tgttccgcag gcaggcccag tgcatcctcc 60
ttggtagagt tttgtgatga tagggttaca catctgcttc aattcctttc tcttatgatc 120
<210> 645
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 645
tctttagcat gtggtttatc acgaagttat ccaatccact ctgaaggata cctttgaaca 60
tagcttcaaa attcttacgc cttttttttt tgccactgtc ttctatcaga aatataaaga 120
<210> 646
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 646
gaatttcgaa tattttatta agggatgatt taattcctgg atttcaaaaa cctttaaaaa 60
catcaaacaa taaactttta taaaaaagtg acaaaataca agttaaatca gctagatttt 120
<210> 647
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 647
ttgcatttgc tctttcatct ctgcttggat tattttaaat cattaggaat taagttatct 60
ttaaaattta agtatctttt ttcaaaaaca ttttttaata gaataaaata taatttgatc 120
<210> 648
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 648
aatggaaatc aggggcagga agagaacagt gtggggtgct aaacagatct cagcaacctc 60
cctgcccacg gccatctctg cgcagacacc tcgacccccc atgcgcaggt ggagcagcgt 120
<210> 649
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 649
gaggaggagg acgcgtctag gctgagatgg ttcgcgtgga tattgctact cattctaaga 60
ctgctgcctc cgccgccgcc gccgccgccg ctgctgccgc cgccgccgcc gccaccattt 120
<210> 650
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 650
atggcagccg tgaccatgtc ggtgcccggg cggaaggcgc cccccaggcc gggcccagtg 60
cccgaggcgg cccagccgtt cctgttcacg ccccgcgggc ccagcgcggg tggcgggcct 120
<210> 651
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 651
ctacttccgg tggtgctgtg gtctgcccct ggagaaccca gaagaacaca gctgtgcgcg 60
cccacaggct ctgggggcgg gagaagataa gtcgcaagga gggggcggga cctacacctc 120
<210> 652
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 652
tcattctgcc ctcgagccca ccgggaacga aagagaagct ctatctcccc tccaggagcc 60
cagctatgaa ctccttctcc acaagtaagt gcaggaaatc cttagccctg gaactgccag 120
<210> 653
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 653
atcagctgcc tcaactgcat ggcccagctc tcacccacca ggcggcacgt gaagatcgag 60
cacgactggc gcagcaccgt gcatggcgcc gtgaagttcg ccttcgactt ctttttcttc 120
<210> 654
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 654
agccccaaag tgagccggcc cagcaagatg aaacagggca ccccagctga cctggtactg 60
aggttgggct gagaccctgc ttctcttccc ccatcaccac ctgcccctcc ctccagccca 120
<210> 655
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 655
gaagtcagtt atatcctggc cgcctttggt ccctcccagg aagacgggca tgttttctgc 60
ttgagaggtg ctgatgtacc agttggggaa ctgggcagac tcaaattcca gcttgttatt 120
<210> 656
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 656
tggagacagg tcctcgcccc ctacaacccc cctgagggcc ccagacccct ctccaggtgg 60
ggttccactg gaggccagtc tgtgtccggc ctccctggca ccctcgggca tctcagagaa 120
<210> 657
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 657
ttttactgac atgcagatgt gctttagagt taatgtttct acaaaaagtt tctataaaca 60
atagaaaatt tctagcatga agtcacagga tgttaaaaat attacaatgc aataaataca 120
<210> 658
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 658
ggccgagccc cgccttgggc ttctgggcgc cctgacttcg ctcccggaac cctcgggcct 60
gggaggcggg aggaagtctt actgagtgca gccccaggcg ccctctcccg ggcctccaga 120
<210> 659
<211> 120
<212> DNA
<213> Synthesis (Artificial synthesis)
<400> 659
taagggcact gccctccttc tcagcttgct cccttaatga ctcctccaac ttgagctttt 60
cagggtctcc gtagcgtaca gtgctgtcac ggaggccact aggagaggcc actttcacaa 120
Claims (2)
1. A probe set for detecting noise-related deafness genes, comprising probes capable of simultaneously and specifically capturing the following 52 noise-related deafness genes: CAT, GSTT1, PON2, SOD1, GSTM1, GSTP1, SOD2, GJB1, GJB2, GJB4, KCNE1, KCNQ4, KCNJ10, HSPA1A, HSPA1B, HSPA1L, CDH23, PCDH15, CASP1, CASP3, CASP4, CASP9, CASP14, CASP5, CASP6, CASP8, CASP10, notch1, KCNMA1, MYH14, OGG1, IL6, WFS1, CLCNKB, IL1B, IL4R, XPO5, APEX1, GSDMEDNMT1, DNMT3A, EYA4, FOXO3, GRHL2, ITGA8, POU4F3, GRM7, HOTABPA, ATP2B2, XYLT1 and GAGAGAYLT 1;
The probe set for capturing the CASP9 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown in SEQ ID No. 1-9;
the probe set for capturing the GJB4 gene comprises 35 oligonucleotide probes, and the base sequences of the 35 oligonucleotide probes are shown as SEQ ID No. 10-44;
the probe set for capturing KCNQ4 genes comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 45-48;
the probe set for capturing the ITGA8 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 49-50;
the probe set for capturing the PCDH15 gene comprises 48 oligonucleotide probes, and the base sequences of the 48 oligonucleotide probes are shown as SEQ ID No. 51-98;
the probe set for capturing CDH23 gene comprises 2 oligonucleotide probes, wherein the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 99-100;
the probe set for capturing KCNQ1 gene comprises 50 oligonucleotide probes, and the base sequences of the 50 oligonucleotide probes are shown as SEQ ID No. 101-150;
the probe set for capturing CAT genes comprises 42 oligonucleotide probes, and the base sequences of the 42 oligonucleotide probes are shown as SEQ ID Nos. 151-192;
The probe set for capturing CASP4 gene comprises 14 oligonucleotide probes, and the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 193-206;
the probe set for capturing the CASP1 gene comprises 5 oligonucleotide probes, and the base sequence of the 5 oligonucleotide probes is shown as SEQ ID No. 207-211;
the probe set for capturing the HOTAIR gene comprises 6 oligonucleotide probes, and the base sequences of the 6 oligonucleotide probes are shown as SEQ ID Nos. 212-217;
the probe set for capturing the GJB2 gene comprises 34 oligonucleotide probes, and the base sequences of the 34 oligonucleotide probes are shown as SEQ ID No. 218-251;
the probe set for capturing the APEX1 gene comprises 2 oligonucleotide probes, and the base sequences of the 2 oligonucleotide probes are shown as SEQ ID Nos. 252-253;
the probe set for capturing the XYLT1 gene comprises 39 oligonucleotide probes, and the base sequences of the 39 oligonucleotide probes are shown as SEQ ID No. 254-292;
the probe set for capturing DNMT1 gene comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 293-300;
the probe set for capturing the CASP14 gene comprises 10 oligonucleotide probes, wherein the base sequences of the 10 oligonucleotide probes are shown as SEQ ID Nos. 301-310;
The probe set for capturing MYH14 genes comprises 8 oligonucleotide probes, and the base sequence of the 8 oligonucleotide probes is shown as SEQ ID No. 311-318;
the probe set for capturing DNMT3A gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 319-325;
the probe set for capturing GABPA genes comprises 13 oligonucleotide probes, and the base sequences of the 13 oligonucleotide probes are shown as SEQ ID No. 326-338;
the probe set for capturing SOD1 gene comprises 20 oligonucleotide probes, and the base sequences of the 20 oligonucleotide probes are shown in SEQ ID No. 339-358;
the probe set for capturing GRM7 gene comprises 28 oligonucleotide probes, and the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 359-386;
the probe set for capturing the ATP2B2 gene comprises 16 oligonucleotide probes, and the base sequence of the 16 oligonucleotide probes is shown as SEQ ID No. 387-402;
the probe set for capturing the CASP3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID No. 403-406;
the probe set for capturing the POU4F3 gene comprises 4 oligonucleotide probes, and the base sequences of the 4 oligonucleotide probes are shown as SEQ ID Nos. 407-410;
The probe set for capturing the HSPA1A gene comprises 79 oligonucleotide probes, and the base sequences of the 79 oligonucleotide probes are shown as SEQ ID No. 411-489;
the probe set for capturing the HSPA1B gene comprises 2 oligonucleotide probes, wherein the base sequences of the 2 oligonucleotide probes are shown as SEQ ID No. 490-491;
the probe group for capturing GSTT1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 492-493;
the probe set for capturing the FOXO3 gene comprises 9 oligonucleotide probes, and the base sequences of the 9 oligonucleotide probes are shown as SEQ ID Nos. 494-502;
the probe set for capturing EYA4 genes comprises 27 oligonucleotide probes, and the base sequences of the 27 oligonucleotide probes are shown as SEQ ID Nos. 503-529;
the probe set for capturing SOD2 gene comprises 20 oligonucleotide probes, and the base sequence of the 20 oligonucleotide probes is shown as SEQ ID No. 530-549;
the probe set for capturing GSDME genes comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No 550-551;
the probe set for capturing the PON2 gene comprises 28 oligonucleotide probes, wherein the base sequence of the 28 oligonucleotide probes is shown as SEQ ID No. 552-579;
The probe set for capturing GRHL2 genes comprises 40 oligonucleotide probes, and the base sequence of the 40 oligonucleotide probes is shown as SEQ ID No. 580-619;
the probe set for capturing the Notch1 gene comprises 2 oligonucleotide probes, and the base sequence of the 2 oligonucleotide probes is shown as SEQ ID No. 620-621;
the probe set for capturing the GJB1 gene comprises 7 oligonucleotide probes, and the base sequence of the 7 oligonucleotide probes is shown as SEQ ID No. 622-628;
the probe set for capturing KCNE1 gene comprises 14 oligonucleotide probes, wherein the base sequence of the 14 oligonucleotide probes is shown as SEQ ID No. 629-642;
the probe set for capturing GSTP1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 643;
the probe set for capturing the HSPA1L gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 644;
the probe set for capturing the CASP5 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 645;
the probe set for capturing CASP6 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 646;
The probe set for capturing CASP8 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 647;
the probe set for capturing the CASP10 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 648;
the probe set for capturing KCNMA1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 649;
the probe set for capturing MYH14 genes comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 650;
the probe set for capturing the OGG1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 651;
the probe set for capturing IL6 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 652;
the probe set for capturing the WFS1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 653;
the probe set for capturing the CLCNKB gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 654;
The probe set for capturing the IL1B gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 655;
the probe set for capturing IL4R gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 656;
the probe set for capturing XPO5 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 657;
the probe set for capturing GSTM1 gene comprises 1 oligonucleotide probe, wherein the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 658;
the probe set for capturing KCNJ10 gene comprises 1 oligonucleotide probe, and the base sequence of the 1 oligonucleotide probe is shown as SEQ ID No. 659.
2. A kit comprising the probe set for detecting a noise-related deafness gene as set forth in claim 1, comprising:
a probe set for detecting a noise-related deafness gene;
an enrichment buffer, wherein the enrichment buffer contains a primer, human cot-1DNA and salmon sperm DNA;
hybridization buffer containing SSPE buffer, denhardt solution, EDTA and SDS;
A binding buffer solution, wherein the binding buffer solution contains NaCl, tris-HCl and EDTA;
a rinse solution 1, wherein the rinse solution 1 contains SSC and SDS;
a rinse solution 2, wherein the rinse solution 2 contains SSC and SDS;
NaOH solution;
Tris-HCl buffer, pH 7.5;
a PCR reaction solution containing dNTPs, a primer, a Phusion buffer solution, a hotspot Phusion enzyme, DMSO and dH 2 O；
TE buffer.
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