CN113943774B - Evaluation method for anti-corrosion efficiency of solid strip-shaped detergent product - Google Patents
Evaluation method for anti-corrosion efficiency of solid strip-shaped detergent product Download PDFInfo
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Abstract
The invention discloses an evaluation method of anti-corrosion efficacy of a solid strip-shaped detergent product, and relates to the technical field of detergent product detection. The evaluation method of the anti-corrosion efficacy of the solid strip-shaped detergent product comprises the following steps: blind holes are formed in the surface of the solid strip-shaped detergent product; preparing a bacterial suspension from freshly cultured bacteria by using sterile pure water and a color-changing synergistic agent, and adding the bacterial suspension into the blind hole; the bacteria at least contain one specific halomonas; and evaluating the antiseptic efficacy of the inoculating blind hole by adopting a color difference value test method. The evaluation method of the anti-corrosion efficiency of the solid strip-shaped detergent product can well evaluate the anti-corrosion efficiency of the solid strip-shaped detergent, provides reliable data support for the efficiency evaluation of the product, and has the advantages of simple operation, strong differentiation and reproducibility, good stability, easy satisfaction of bacterial culture conditions and lower cost.
Description
Technical Field
The invention relates to the technical field of detection of detergent products, in particular to an evaluation method of anti-corrosion performance of a solid strip-shaped detergent product.
Background
The solid detergent products such as the laundry soap are the washing products with the longest use history for human beings, can be used for washing clothes, removing stains on the clothes, keeping the clothes clean and bright, and can deeply remove dirt on human and animal bodies and hair. With the progress of technology and the development of society, people are not only required to simply clean and decontaminate clothes, clean hair and skin, but also hope that the washing product not only meets the conditions of greenness, naturalness, mildness, easy degradation and the like, but also can add more functions, so that higher requirements are put forward for product developers, the product system is milder, and the product quality is more resistant to multiple risks.
The use environment and the use mode of solid bar detergents such as soaps are very different from those of liquid detergents and solid particle detergents (powder), the solid bar detergents are exposed to the air after the package is removed and the package is started to be used, even the wet corners of a toilet with very high microorganism content are exposed, and the storage environment greatly increases the risk of microorganism infection of the solid detergents. In the use process, the solid strip-shaped detergent is in long-term direct contact with a large amount of microorganisms on clothes, so that the microorganisms on the products are greatly proliferated, and the risk of mass-proliferation infection of the microorganisms is increased by opening a moist storage environment. The problem of color change in the use process of the laundry soap is more common, and the deterioration and color change phenomena caused by environmental microorganism infection are more common.
The shape of the solid bar-shaped detergent such as soap cannot realize uniform stirring and mixing after adding bacteria and subsequent monitoring of the number of living bacteria, so that the microbial challenge performance evaluation is difficult. The related research in the industry is currently blank.
In addition, most of soaps are infected with microorganisms locally, and the appearance of the infected area is usually that spots with darker colors appear, so that the situation that the whole area of the soap is discolored due to microbial infection is less likely to appear.
In summary, an evaluation method with simple operation, strong distinguishing property, strong reproducibility and reliable result is required to be researched aiming at the microorganism challenge efficacy of the solid strip-shaped detergent, so that the loss caused by microorganism pollution is avoided, and reliable guarantee is provided for guaranteeing the health of consumers.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide an evaluation method for the anti-corrosion efficiency of a solid strip-shaped detergent, which can evaluate the efficiency of related products truly, accurately and reliably.
Meanwhile, another object of the present invention is to provide a method for local microorganism infection, which fills the gap in the prior art.
A method for evaluating the preservative efficacy of a solid bar detergent product, comprising the steps of:
step 1: blind holes are formed in the surface of the solid strip-shaped detergent product;
step 2: preparing a bacterial suspension from freshly cultured bacteria by using sterile pure water and a color-changing synergistic agent by adopting a local microorganism infection method, and adding the bacterial suspension into the blind hole in the step 1; the bacteria at least contain one specific halomonas;
step 3: evaluating the corrosion resistance; and adopting a color difference value test method to detect the color difference value of the inoculating blind hole, wherein the method specifically comprises the following steps:
on the 14 th day after bacteria inoculation, the color difference value of the inoculated blind holes is more than 10, and the sample anticorrosion efficiency is unqualified;
the color difference value of the inoculating blind hole is more than 10 within 15 days to 28 days after bacteria are inoculated, so that the sample anticorrosion efficacy is qualified;
and on the 28 th day after bacteria inoculation, the color difference value of the inoculated blind holes is less than or equal to 10, so that the sample has good anti-corrosion effect.
Step 4: repeating the steps 1 to 3 twice, namely carrying out three parallel tests on the same sample, and when the results of the three tests have no grade difference, evaluating that the test results are effective, discarding the results with the grade difference, and reserving the results without the grade difference; and when the results of any two of the three tests have grade differences, the test results are judged to be invalid.
Further, in the step 3, the anticorrosion efficiency is evaluated, and the method further comprises the step of adopting a visual method to judge the color of the inoculated blind hole after the treatment in the step 2, specifically:
on day 14 after bacteria inoculation, obvious color change occurs in the inoculation blind hole, so that the sample anticorrosion efficiency is unqualified;
the method comprises the steps that after bacteria are inoculated, no obvious color change exists in an inoculation blind hole, and the sample anticorrosion efficacy is qualified when the obvious color change exists in the inoculation blind hole within 15 days to 28 days after bacteria are inoculated;
on day 28 after bacterial inoculation, no obvious color change appears in the blind inoculation holes, so that the sample has good antiseptic efficacy.
Preferably, in step 1, the solid detergent bar is a block with a specific side length obtained by cutting the solid detergent bar; the block is cuboid, the sides S1, S2 and S3 respectively represent the length, width and height of the cuboid, a plurality of blind holes are dug on one side plane surface formed by the sides S1 and S2, the blind holes do not penetrate through two opposite side surfaces of the block, and the depth of the blind holes is smaller than the side length of the side S3. Preferably, the block has a side S1 longer than a side S2, the side S2 having a length of 2.5cm or more and the side S3 having a length of 0.7 to 0.9cm;
preferably, the blind holes are triangular blind holes, round blind holes, semi-elliptic blind holes, square blind holes, rectangular blind holes, trapezoid blind holes or any polygonal blind holes with sides number larger than four.
Further, the plane formed by the side S1 and the side S2 of the blind hole in the step 1 is selected from triangle, circle, semi-ellipse, square, rectangle, trapezoid and any polygon with the number of sides greater than 4, and the projection shape of the plane formed by the side S1 and the side S3 of the blind hole is triangle, semi-circle, semi-ellipse, square, rectangle, trapezoid and any polygon with the number of sides greater than 4. The pattern circumference of the blind hole on one side plane formed by the side S1 and the side S2 is more than or equal to 3cm. The number of the blind holes is more than or equal to three, the distance between the center and the side S1 and the distance between the center and the side S2 are more than or equal to 1cm, and the distance between the centers of two adjacent blind holes is more than or equal to 1.5cm.
Further, the circumference of the mouth of the blind hole is larger than 3cm, and the depth is 0.5-0.7cm.
Preferably, in the step 2, the bacteria are mixed bacteria; the mixed bacteria comprise specific halomonas and one or more of unspecified bacteria; the specific halomonas is halomonas still; the nonspecific bacteria include at least one of Staphylococcus aureus, escherichia coli, enterobacter cloacae, pseudomonas aeruginosa, salmonella other than Salmonella still, and Lactobacillus niveus.
Further, in the step 2, fresh cultivation means that each kind of bacteria is respectively coated on a tryptone soybean agar medium plate, and the lawn after 1 day of cultivation in an incubator at 35 ℃ to 37 ℃ is the fresh cultivated microorganism, and the lawn is washed by 10ml of color-changing synergist to prepare a bacterial suspension, and the bacterial suspension is the fresh cultivated bacterial suspension.
Further, in the step 2, the color-changing synergist is an aqueous solution of one or a mixture of two of n-butanol and propylene glycol, and the mass concentration of the n-butanol and/or the propylene glycol is 1-3%.
Further, the bacterial suspension in the blind hole has the bacterial adding amount of 200 to 300 mu L/hole, and the viable count in the blind hole is 1 multiplied by 10 9 CFU/well to 9X 10 9 CFU/well.
Preferably, the color of the inoculating blind hole treated in the step 2 is judged by adopting the visual method to evaluate the corrosion resistance, and the method specifically comprises the following steps:
on the 14 th day after bacteria inoculation, the inoculation blind hole has obvious brown color, and the sample anticorrosion efficacy is unqualified;
the day 14 after bacteria inoculation, the blind holes are not obviously brown, and the blind holes are obviously brown within 15 to 28 days after bacteria inoculation, so that the sample anticorrosion efficacy is qualified;
on day 28 after bacterial inoculation, no obvious brown color appears in the blind inoculation holes, so that the sample has good antiseptic efficacy.
According to the evaluation method of the anti-corrosion effect of the solid strip-shaped detergent product, blind holes are formed in the surface of the solid strip-shaped detergent product; preparing a bacterial suspension from freshly cultured bacteria by using sterile pure water and a color-changing synergistic agent, and adding the bacterial suspension into the blind hole; the bacteria at least contain one specific halomonas; evaluating the corrosion resistance; evaluating the antiseptic efficacy of the inoculating blind hole by adopting a color difference value test method; repeating the steps, carrying out three parallel tests on the same sample, and retaining effective data.
Compared with the prior art, the invention has the following technical effects:
1. the evaluation method provided by the invention adopts specific microorganisms, can well evaluate the anti-corrosion efficacy of the solid strip-shaped detergent, and provides reliable data support for evaluating the efficacy of the product.
2. The evaluation method provided by the invention is simple and convenient to operate, strong in distinguishing property and strong in reproducibility.
3. The evaluation method provided by the invention has good stability, easy satisfaction of bacterial culture conditions and low cost.
Biological material preservation information
The biological material name of halomonas stevensis; latin name halomonas stevensii; the collection of microorganism strains (GDMCC) of Guangdong province, the collection address of building 5, no. 59, no. 5, no. 11, no. 2021, and the collection number of GDMCC 61659.
Drawings
In order to more clearly illustrate the method of evaluating the preservative efficacy of a solid bar detergent product of the present invention, the drawings required for the step descriptions of the evaluation method will be briefly described, and it will be apparent to those skilled in the art that the drawings in the following description are some embodiments of the present invention and that other drawings can be obtained according to these drawings without the need for inventive effort.
Fig. 1 is a schematic view of a solid bar detergent product (bar) of the present invention in examples, comparative examples.
Fig. 2 is a schematic view of the solid detergent bar (block) of fig. 1 after blind holes are cut in the plane consisting of sides S1 and S2.
Fig. 3 is a schematic cross-sectional view of the solid bar detergent product (bar) of fig. 1.
FIG. 4 is a schematic representation of sample soap 1 of example 1 with a significant discoloration after blind inoculation.
Fig. 5 is a schematic representation of sample soap 1 in comparative example 9 without significant discoloration after blind inoculation.
Fig. 6 is a schematic diagram showing the difference in discoloration of sample soaps 1 to 4 after inoculation in examples 5 to 8.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples, but the embodiments of the present invention are not limited thereto.
The features, advantages and advantages of the present invention will become apparent to those skilled in the art from a reading of the present disclosure.
All percentages, fractions and ratios are by weight of the total composition of the present invention, unless otherwise specified. All weights as they pertain to listed ingredients are to the active level and, therefore, do not include solvents or by-products that may be included in commercially available materials, unless otherwise specified. The term "weight content" is used herein to denote the symbol "%".
The terms "comprising," "including," "containing," "having," or other variations thereof herein are intended to cover a non-closed inclusion, without distinguishing between them. The term "comprising" means that other steps and ingredients may be added that do not affect the end result. The term "comprising" also includes the terms "consisting of …" and "consisting essentially of …". The methods/processes of the present invention can comprise, consist of, and consist essentially of the essential elements and limitations described herein, as well as additional or optional ingredients, components, steps, or limitations of any of the embodiments described herein.
The terms "efficacy," "performance," "effect," "efficacy" are not differentiated herein.
The term "not comprising" herein means not being added by man.
Solid bar washAgent
The terms "solid bar detergent", "soap product" according to the present invention do not distinguish between them, solid bar detergents include, but are not limited to, the following products: laundry soaps, underwear soaps, and the like.
Preservative efficacy
The terms "preservative efficacy", "microbial challenge resistance efficacy", "anti-discoloration efficacy", "discoloration challenge resistance efficacy" in the present invention do not distinguish between. Preservative performance refers to the ability of a product to resist microbial infection.
Corrosion resistance evaluation method
The terms "evaluation method of preservative efficacy", "preservative challenge", "microbial challenge test" in the present invention do not distinguish between. The method for evaluating the antiseptic efficacy specifically refers to that the sample is subjected to microbial infection, and the capability of the sample for resisting the microbial infection is evaluated by means of appearance change, smell change, microbial propagation, survival condition and the like of the sample.
The microorganism involved in the method for evaluating the preservative efficacy in the invention is bacteria. Bacteria include specific halomonas bacteria, standard bacterial species and other bacterial strains, among other unspecific bacteria.
According to the treatment form of the sample in the method for evaluating the anti-corrosion performance, the method for evaluating the anti-corrosion performance is a local infection method.
Local infection method
The term "local infection method" according to the present invention is one of methods for evaluating antiseptic efficacy, and specifically refers to a method in which, when a sample is subjected to microbial infection, microbial inoculation infection is performed only on some specific areas of the sample. I.e., the sample has both a microorganism-infected area and a microorganism-infected area. Subsequently, the ability of the samples to resist microbial infection was evaluated by means of appearance changes, odor changes, microbial proliferation and survival.
The inventor adopts a local infection method to evaluate the anti-corrosion efficacy according to the shape characteristics, actual use and actual bacterial contamination conditions of the solid strip-shaped detergent.
Specific halomonas
The term "specific halomonas" according to the present invention is halomonas still (halomonas stevensii) which has been deposited at the cantonese microbiological strain collection center under the accession number GDMCC 61659 on month 11 of 2021. The mixed bacteria for local infection of solid bar detergents of the invention comprise at least specific halomonas.
Unspecific bacteria
The term "non-specific bacteria" of the present invention includes, but is not limited to, staphylococcus aureus, escherichia coli, enterobacter cloacae, pseudomonas aeruginosa, other Salmonella, and Zygosaccharomyces niveus; no specific halomonas was included. The term "other halomonas" refers to a bacterium of the genus halomonas, but does not include the specific halomonas described above.
Mixed bacteria
The term "mixed bacteria" according to the invention comprises at least specific halomonas bacteria and may also comprise one or more of the non-specific bacteria. The microorganism used for infecting the solid strip-shaped detergent is mixed bacteria. The mixed bacteria infect the solid strip-shaped detergent in the form of bacterial suspension, and the antiseptic effect of the solid strip-shaped detergent is qualitatively evaluated through the color change of the inoculated holes after culture.
Block material
The term "cake" according to instant invention shall mean in particular an object having a specific side length, obtained by cutting a solid detergent bar product. The block shown in fig. 1-3 is specifically a cuboid, the sides S1, S2 and S3 respectively represent the length, width and height of the cuboid, the length of the side S1 is greater than that of the side S2, the length of the side S2 is greater than or equal to 2.5cm, and the length of the side S3 is 0.7-0.9 cm.
Hole(s)
The terms "blind", "well", "inoculation well", "local infection area" in the present invention do not distinguish between them. Kong Juti refers to a localized area at the surface of the mass with a depth for inoculating microorganisms. A plurality of holes are dug on one side plane surface formed by the sides S1 and S2 of the block, and the holes do not penetrate through two opposite side surfaces of the block.
The depth of the hole is smaller than the side length of the side S3. The shape of the plane formed by the side S1 and the side S2 of the hole is selected from triangle, circle, semi-ellipse, square, rectangle, trapezoid and any polygon with the number of sides being more than 4, and the projection shape of the plane formed by the side S1 and the side S3 of the hole is selected from triangle, semicircle, semi-ellipse, square, rectangle, trapezoid and any polygon with the number of sides being more than 4; the pattern circumference of the hole on one side plane formed by the side S1 and the side S2 is more than or equal to 3cm; the number of holes is more than or equal to 3; the distance between the center of the hole and the side S1 and the distance between the side S2 are greater than or equal to 1cm, and the distance between the centers of the two holes is greater than or equal to 1.5cm.
Bacterial suspension and color-changing synergist
The term "bacterial suspension" in the present invention refers to a mixture of mixed bacteria, a color-changing synergist and sterile pure water.
The term "colour change potentiator" according to the invention is selected from n-butanol and propylene glycol at a concentration of 1% to 3% in the bacterial suspension. The color-changing synergist can reduce the color-changing time of the inoculated holes after bacteria infection and improve the color-changing speed.
The bacterial suspension is added with 200 to 300 mu L/hole, and the viable count is 1 multiplied by 10 9 CFU/well to 9X 10 9 CFU/well.
Color change
The term "discoloration" according to the present invention refers to the phenomenon of change in apparent color caused by contact of a solid bar detergent with a microorganism. The term "contacting" encompasses both the artificially inoculated form and the non-inoculated form.
Visual method
The term "visual method" according to the present invention refers to evaluating the preservative efficacy of a sample by visually inspecting the discoloration of an inoculated well on the sample at a specific time. In order to ensure the effectiveness of the data, the method for evaluating the anti-corrosion efficiency provided by the invention needs to carry out three parallel tests on the same sample, and when the results of the three tests have no grade difference, the test data are evaluated to be effective, and the results with grade difference are discarded at the moment, and the results without grade difference are reserved. When there is a grade difference between any two results of the three tests, the test data is judged to be invalid.
Color difference value testing method
The term "color difference value test method" refers to a method for testing the color difference value (dE x ab (D65)) of an inoculated hole on a sample at a specific time by using a color difference meter, and evaluating the corrosion resistance of the sample according to the size of the color difference value. The specific test method comprises the following steps: placing the sample blocks with the inoculation holes with color change or without color change for 28 days into transparent self-sealing bags on an ultra-clean workbench, sealing the transparent self-sealing bags to prevent living bacteria of the inoculation holes from polluting the environment, and using a color difference meter with the diameter of a measured caliber of about 8mm to align the inoculation holes for color difference value test, wherein the color difference value test standard sample of each sample block inoculation hole is the same sample and the same size of the non-inoculated inoculation hole.
In order to ensure the effectiveness of data, the method for evaluating the anti-corrosion efficiency provided by the invention needs to perform three parallel tests on the same sample, and takes the average value of the color difference values of the three parallel tests as the test result of the color difference value.
Corrosion prevention efficiency evaluation method
The evaluation method of the anti-corrosion efficiency in the invention is to evaluate the anti-corrosion efficiency of the solid detergent by using the results of a visual method and a color difference value test method at a specific time. Specifically, specific time and preservative efficacy evaluation grades are shown in table 1 below.
Table 1 evaluation of preservative efficacy of solid bar detergents
Culture, medium and fresh culture
The term "culture" according to the present invention is intended to provide the external conditions required for the growth, maintenance and propagation of bacteria, including specific temperature, humidity, nutritional conditions, etc.
The term "medium" according to the present invention refers to a nutrient condition that provides the bacteria with the need for growth, maintenance and reproduction, and which generally contains carbohydrates, nitrogen-containing substances, inorganic salts, moisture, etc., including liquid media and solid agar media.
The term "fresh culture" of the present invention means that each kind of bacteria is respectively coated on a tryptone soy agar medium (TSA) plate, and the lawn after 1 day of culture in a 35-37 ℃ incubator is a fresh cultured microorganism, and the lawn is washed with 10ml of a color-changing synergist aqueous solution to prepare a bacterial suspension, and the bacterial suspension is a fresh cultured bacterial suspension, which can be inoculated in an inoculation hole for testing.
Without further elaboration, it is believed that one skilled in the art can, using the preceding description, utilize the present invention to its fullest extent. The following examples are intended to further describe and demonstrate embodiments within the scope of the present invention. Accordingly, the examples should be construed as merely illustrative of the invention in greater detail and not limiting the invention in any way.
In the following examples and comparative examples, all contents are by weight unless otherwise indicated, and the contents of the ingredients listed are the contents of the active substances in terms of conversion.
The information of the solid bar detergent samples used in the examples and comparative examples of the present invention are shown in table 2 below.
TABLE 2 sample information
| Sample numbering | Sample information |
| Sample soap 1 | Solid bar detergent 1, laundry soap, yellow, preservative free |
| Sample soap 2 | Solid bar detergent 2, laundry soap, yellow, containing 20ppm benzisothiazolinone |
| Sample soap 3 | Solid bar detergent 3, commercially available laundry soap, yellow, containing 80ppm benzisothiazolinone |
| Sample soap 4 | Solid bar detergent 4, commercially available laundry soap, white, containing 120ppm benzisothiazolinone |
Preparation and perforation of sample blocks in examples 1 to 16 and comparative examples 1 to 16
Sample soaps 1 to 4 were cut into pieces, which were rectangular parallelepiped, side S1 was 5.5cm, side S2 was 2.8cm, side S3 was 0.8cm, blind holes were dug in the surface of one side plane constituted by side S1 and side S2, the shape of the holes in the plane constituted by side S1 and side S2 was circular, and the circumference was about 3cm. The projected shape of the hole in the plane formed by the sides S1 and S3 is semicircular. The number of holes in each block is 3; the distance between the center of the hole and the side S1 is 1.2 to 1.5cm, the distance between the center of the hole and the side S2 is 1 to 1.4cm, and the center distance between the two holes is 1.8 to 2 cm. The depth of the blind holes of the block is 0.6cm to 0.7cm.
Preparation and perforation of sample soap bars in examples 17 to 20 and comparative examples 17 to 20
Sample soaps 1 to 4 were cut into pieces, which were rectangular parallelepiped, side S1 was 5.5cm, side S2 was 2.8cm, side S3 was 0.8cm, blind holes were dug in the surface of one side plane constituted by side S1 and side S2, and the shape of the hole in the plane constituted by side S1 and side S2 was a square with a side length of 10 mm. The projected shape of the hole on the plane formed by the sides S1 and S3 is rectangular. The number of holes in each block is 3; the distance between the center of the hole and the side S1 is 1.2 to 1.5cm, the distance between the center of the hole and the side S2 is 1 to 1.4cm, and the center distance between the two holes is 1.8 to 2 cm. The depth of the blind holes of the block is 0.6cm to 0.7cm.
Evaluation results after the sample was infected with a specific halomonas strain containing different color-changing synergists
Examples 1 to 8 and comparative examples 1 to 8 used a microbial local infection method using freshly cultured specific halomonas (guarantor)Monomonas still with the Tibetan number of GDMCC 61659 (the added amount of the bacterial suspension is 250 mu L/hole, and the number of viable bacteria in a blind hole is 4 multiplied by 10) 9 CFU/well to 5X 10 9 CFU/well) the sample soaps 1 to 4 were subjected to preservative efficacy evaluation. The results are shown in Table 3.
The results of the apparent discoloration of sample soap 1 in example 1 after blind inoculation are shown in FIG. 4, and the differences in discoloration of sample soaps 1 to 4 in examples 5 to 8 after inoculation are shown in FIG. 6. The test results show that when the sample is subjected to local infection by using the single bacterial suspension of the specific halomonas, the color change time of the sample soaps 1 to 4 is obviously different, which indicates that obvious preservative efficacy difference exists between the sample soaps indeed.
Examples 1 to 8 employed a color change synergist, greatly shortening the test time. Comparative examples 1 to 4 do not employ a discoloration enhancer, and the discoloration time is significantly longer than in examples. Comparative examples 5 to 8 the discoloration synergists of the present invention were replaced with PEG400, which on the one hand did not shorten the discoloration time and on the other hand resulted in a test result that resulted in false negatives of sample soap 2, indicating that PEG400 could not be used as a suitable discoloration synergist.
TABLE 3 influence of color-changing synergists on the results of evaluation of preservative efficacy of samples
Evaluation results after infection of samples with a Mixed bacterial solution containing a specific Salmonella
Examples 9 to 16 and comparative examples 9 to 16 used a microbial topical infection method using freshly cultured specific halomonas (Steve accession number GDMCC 61659) with color change potentiatorHalomonas) and a mixed bacterial suspension of non-specific bacteria (the bacterial suspension added bacterial amount is 250 mu L/hole, and the number of viable bacteria in a blind hole is 4 multiplied by 10) 9 CFU/well to 5X 10 9 CFU/well) the sample soaps 1 to 4 were subjected to preservative efficacy evaluation. The results are shown in Table 4.
The results of comparative example 9, in which sample soap 1 was not significantly discolored after blind inoculation, are shown in fig. 5. As can be seen from the test results, the samples of examples 9 to 16 were locally infected with different mixed bacterial suspensions containing specific halomonas respectively, and the color change times of the sample soaps 1 to 4 were all obvious, which indicates that there was indeed a significant difference in antiseptic efficacy between the sample soaps. However, when the mixed liquor does not contain specific halomonas, the preservative efficacy of the soap of comparative examples 9 to 16,4 cannot be clearly distinguished. The technical scheme of the invention can effectively distinguish the antiseptic efficacy of soap samples only by adopting specific halomonas.
TABLE 4 influence of specific Salmonella on the results of evaluation of preservative efficacy of samples
Influence of the shape of the perforations in the sample block on the evaluation results
The shape of the perforations in the sample blocks of examples 17 to 20 and comparative examples 17 to 20 was square, and the evaluation results are shown in table 5.
As can be seen from a comparison of Table 4 (examples 9 to 12) and Table 5 (examples 17 to 20), the difference in color change time between sample soaps 1 to 4 is evident regardless of whether the punched hole shape is circular or square. Similarly, tables 4 (comparative examples 9 to 12) and 5 (comparative examples 17 to 20) show that when the mixed bacterial liquid does not contain specific halomonas, the preservative efficacy of 4 sample soaps cannot be clearly distinguished regardless of whether the sample cake has a round or square perforated shape.
TABLE 5 influence of sample block perforation shape on evaluation results
The dimensions and values disclosed herein are not to be understood as being strictly limited to the exact numerical values recited. Unless otherwise indicated, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40mm" is intended to mean "about 40mm".
All documents cited in the summary are incorporated by reference in the relevant section. Citation of any document is not to be construed as an admission that it is prior art with respect to the present invention.
The present invention is not limited to the preferred embodiments, but can be modified, equivalent, and modified in any way without departing from the technical scope of the present invention.
Claims (4)
1. The method for evaluating the anti-corrosion efficiency of the solid strip-shaped detergent product is characterized by comprising the following steps of:
step 1: blind holes are formed in the surface of the solid strip-shaped detergent product; the circumference of the mouth part of the blind hole is more than 3cm, and the depth is 0.5-0.7cm;
step 2: preparing a bacterial suspension from freshly cultured bacteria by using sterile pure water and a color-changing synergistic agent, and adding the bacterial suspension into the blind hole in the step 1; the bacteria are Salmonella stillhalomonas stevensii) Or halomonas stillhalomonas stevensii) Mixed bacteria of staphylococcus aureus, escherichia coli, enterobacter cloacae and pseudomonas aeruginosa; the halomonas still is describedhalomonas stevensii) The deposit number is GDMCC 61659; the bacterial suspension in the blind hole has the bacterial adding amount of 200 to 300 mu L/hole, and the viable count in the blind hole is 1 multiplied by 10 9 CFU/well to 9X 10 9 CFU/well; the color-changing synergist is n-butanol or propylene glycol, and the mass concentration of the n-butanol or the propylene glycol is 1-3%;
step 3: and (3) detecting the color difference value of the inoculating blind hole by adopting a color difference value test method, and evaluating the anti-corrosion efficiency according to the detection result.
2. The method for evaluating the preservative efficacy of a solid bar detergent product according to claim 1, wherein in the step 2, freshly cultured bacteria are prepared by respectively coating each of the bacteria on a tryptone soybean agar medium plate, and culturing the bacteria in an incubator at 35 ℃ to 37 ℃ for 1 day; the lawn was washed with 10ml of color-changing synergist to prepare a bacterial suspension.
3. The method for evaluating the anti-corrosion performance of the solid detergent bar product according to claim 2, wherein the blind holes are triangular blind holes, round blind holes, semi-elliptical blind holes, square blind holes, rectangular blind holes, trapezoidal blind holes or any polygonal blind holes with sides more than four.
4. A method of evaluating the preservative efficacy of a solid bar detergent product as claimed in any one of claims 1 to 3, further comprising the step of 4: repeating the steps 1 to 3 twice, namely carrying out three parallel tests on the same sample, and when the results of the three tests have no grade difference, evaluating that the test results are effective, discarding the results with the grade difference, and reserving the results without the grade difference; and when any two results in the three tests have grade differences, the test result is judged to be invalid.
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| CN111304281A (en) * | 2020-03-31 | 2020-06-19 | 广州立白企业集团有限公司 | Evaluation method of corrosion resistance |
| CN111398260A (en) * | 2020-03-31 | 2020-07-10 | 广州立白企业集团有限公司 | Method for rapidly evaluating antibacterial efficacy and method for displaying antibacterial efficacy on surface of carrier |
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| CN111304281A (en) * | 2020-03-31 | 2020-06-19 | 广州立白企业集团有限公司 | Evaluation method of corrosion resistance |
| CN111398260A (en) * | 2020-03-31 | 2020-07-10 | 广州立白企业集团有限公司 | Method for rapidly evaluating antibacterial efficacy and method for displaying antibacterial efficacy on surface of carrier |
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