CN113943658A

CN113943658A - Cell culture bottle

Info

Publication number: CN113943658A
Application number: CN202111441740.5A
Authority: CN
Inventors: 丁贤明
Original assignee: Suning Suzhou Biotechnology Co Ltd
Current assignee: Suning Suzhou Biotechnology Co Ltd
Priority date: 2021-11-30
Filing date: 2021-11-30
Publication date: 2022-01-18
Anticipated expiration: 2041-11-30
Also published as: CN113943658B

Abstract

The invention discloses a cell culture bottle. The cell culture bottle comprises a bottle body and a bottle cap, a bottle opening is formed in the bottle body, a bottle edge is fixedly arranged outside the bottle body, an extending sealing part is arranged on the bottle cap, air holes penetrating through the extending sealing part are formed in the extending sealing part, the extending sealing part can generate recoverable deformation along a first direction and a second direction under external force compression, and when the bottle cap is closed at the bottle opening along the first direction, the extending sealing part can be abutted to the bottle edge, and the bottle cap is configured to be in a fully-opened state, a semi-sealed state and a fully-sealed state. According to the cell culture bottle provided by the invention, the ventilation adjusting structure and the inner sealing structure are arranged between the bottle body and the bottle cap, so that the required ventilation quantity is convenient to adjust, the requirements of various ventilation quantities can be met only by one cell culture bottle, the product utilization rate is improved, and the cell culture bottle is easy to popularize.

Description

Cell culture bottle

Technical Field

The invention particularly relates to a cell culture bottle, and belongs to the technical field of cell culture bottles.

Background

The cell culture bottle is an important device for drug tests and implantable medical device tests, and the tightness and safety of the cell culture bottle are quite high, so that the cell culture bottle is widely favored by researchers in the pharmaceutical industry and the medical device industry. Cell culture flasks can be divided into: the shapes of the bottoms of the glass cell culture bottles and the plastic cell culture bottles can be round, square, rectangle, triangle and the like. The volume of the cell culture bottle has various specifications such as 500ml to 3L.

At present, the cell culture bottle on the market comprises a bottle body and a bottle cap detachably connected with the bottle body. Traditional cell culture bottle switches the use through two kinds of bottle lids to adapt to different cultivation modes, but such switching mode, the operation is comparatively loaded down with trivial details, but has still increased the cost of production, and the practicality is relatively poor, is unfavorable for sustainable development.

Disclosure of Invention

The invention mainly aims to provide a cell culture bottle to overcome the defects in the prior art.

In order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps:

the embodiment of the invention provides a cell culture bottle, which comprises a bottle body and a bottle cap, wherein a bottle opening is formed on the bottle body, the bottle cap can be opened and closed at the bottle opening,

the bottle comprises a bottle body and a bottle cover, and is characterized in that a bottle edge is fixedly arranged outside the bottle body, an extension sealing part is arranged on the bottle cover, air holes penetrating through the extension sealing part are formed in the extension sealing part, the extension sealing part can generate recoverable deformation along a first direction and a second direction under the compression of external force, when the bottle cover is covered at a bottle opening along the first direction, the extension sealing part can be abutted against the bottle edge, and the bottle cover is configured into a fully-opened state, a semi-sealed state and a fully-sealed state;

when the bottle cap is in a fully opened state, the first area on the surface of the extension sealing part is in contact with the bottle edge, the cavity inside the bottle body can be communicated with the outside through a gap between the bottle body and the bottle cap and the air vent, and the air vent is provided with a first aperture;

when the bottle cap is in a semi-sealing state, the second area on the surface of the extension sealing part is in close contact with the bottle edge, the cavity inside the bottle body can be communicated with the outside through a gap between the bottle body and the bottle cap and the air vent, the air vent is provided with a second aperture, and the second aperture is smaller than the first aperture;

when the bottle lid is in full seal state, extend the third region on sealing member surface with bottle edge in close contact with, the inside cavity of body is kept apart with the external world, wherein, the bleeder vent is located between third region and first region/second region, perhaps, the bleeder vent is located the third region.

Compared with the prior art, the cell culture bottle provided by the embodiment of the invention has the advantages that the ventilation adjusting structure and the inner sealing structure are arranged between the bottle body and the bottle cap, the needed ventilation quantity is convenient to adjust, the structure is simple, the practicability is high, the requirements of various ventilation quantities can be met by only one cell culture bottle, the product utilization rate is improved, the popularization is easy, in addition, the cell culture bottle provided by the embodiment of the invention has good identification degree through simple marking, and the practicability is higher.

Drawings

FIG. 1 is a schematic structural view of a cell culture flask according to an embodiment of the present invention in a fully opened state;

FIG. 2 is an enlarged schematic view of FIG. 1 at configuration A;

FIG. 3 is a schematic diagram of a cell culture flask according to an embodiment of the present invention in a semi-sealed or semi-opened state;

FIG. 4 is an enlarged schematic view of FIG. 3 at configuration A;

FIG. 5 is a schematic structural view of a cell culture flask according to an embodiment of the present invention in a completely sealed state;

FIG. 6 is an enlarged schematic view of FIG. 5 at configuration A;

FIG. 7 is a schematic structural diagram of a sealing ring in a cell culture flask according to an embodiment of the present invention.

Detailed Description

In view of the deficiencies in the prior art, the inventors of the present invention have made extensive studies and extensive practices to provide technical solutions of the present invention. The technical solution, its implementation and principles, etc. will be further explained as follows.

In a more specific embodiment, at least the portion of the extended sealing member adjacent the rim of the bottle is inclined or curved in a direction away from the body; and/or one end of the extension sealing component close to the bottle edge is provided with an arc-shaped guide contact surface, and the first area is positioned on the guide contact surface.

In a more specific embodiment, the extension sealing means is an annular member provided at the peripheral edge of the closure, or the extension sealing means is formed by extending the peripheral edge of the closure.

In a more specific embodiment, at least the portion of the extended sealing member adjacent the rim of the bottle is a flexible member.

In a more specific embodiment, the rim is an annular member fixedly disposed at an outer peripheral edge portion of the body.

In a more specific embodiment, the body, cap, elongate sealing member and bottle rim are all coaxially disposed.

In a more specific embodiment, a sealing ring is further disposed inside the bottle cap, and when the bottle cap is closed at the bottle opening and configured in a fully sealed state, the sealing ring can be in sealing contact with the bottle opening.

In a more specific embodiment, the sealing ring has a first contact surface, the bottleneck of the bottle body has a second contact surface, and when the bottle cap is in a fully sealed state, the first contact surface can be in sealing fit with the second contact surface.

In a more specific embodiment, the first contact surface and the second contact surface are both inclined surfaces that are obliquely arranged.

In a more specific embodiment, the first contact surface includes a plurality of arc structures disposed at intervals, and a groove structure is formed between two adjacent arc structures, wherein the arc structures can be deformed to be restorable under external force, and when the arc structures are compressed, a deformed portion of the arc structures generated by compression can extend into the groove structure to completely close the groove structure.

In a more specific embodiment, the arc-shaped structure has an arc-shaped contact surface capable of being in sealing fit with the second contact surface.

In a more specific embodiment, the arc-like structure is a flexible member.

In a more specific embodiment, the bottle cap is connected with the bottle body in a threaded manner, an internal thread structure for connecting with the bottle body is arranged at a first position on the bottle cap, and a joint of the extension sealing part and the bottle cap is provided with a second position;

when the bottle cap is in a fully opened state, the vertical distance between the first position and the bottle edge is H, the vertical distance between the second position and the bottle edge is H1, a gap is formed between the first contact surface and the second contact surface, the vertical distance between the first contact surface and the second contact surface is K1, and H1 is 0.5H is 2K 1;

when the bottle cap is in a semi-sealing state, the extending distance B2 of the extending sealing part on the bottle edge is 0.5B, the vertical distance H2 between the second position and the bottle edge is 0.25H, the arc-shaped structure of the first contact surface is abutted against the second contact surface, and an airflow channel for airflow is formed between the groove structure and the second contact surface;

when the bottle cap is in a fully sealed state, the extending distance B3 of the extending sealing part on the bottle edge is 0.8B, the vertical distance H3 between the second position and the bottle edge is 0.2H, the arc-shaped structure of the first contact surface is in sealing contact with the second contact surface, and the groove structure is completely filled by the arc-shaped structure, wherein B is the length of the bottle edge in the second direction.

In a more specific embodiment, the first direction is an axial direction of the body and the cap, and the second direction is a radial direction of the body and the cap.

In a more specific embodiment, the bottle body is further provided with a plurality of adjusting marks, and the adjusting marks respectively correspond to a fully open state, a semi-sealed state and a fully sealed state.

In a more specific embodiment, the bottle cap is a transparent member.

In the following, the technical scheme, the implementation process and the principle thereof will be further explained with reference to the drawings, and unless otherwise specified, the material and the like in the cell culture flask in the embodiment of the present invention may be selected according to specific requirements, and is not specifically limited herein.

Spatially relative terms, such as "upper," "lower," "left," "right," and the like, may be used in the embodiments for ease of description to describe one element or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatial terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "below" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "lower" can encompass both an upper and a lower orientation. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

Moreover, relational terms such as "first" and "second," and the like, may be used solely to distinguish one element from another element having the same name, without necessarily requiring or implying any actual such relationship or order between such elements.

Referring to fig. 1, 2 and 7, a cell culture bottle includes a bottle body 1 and a bottle cap 2, a bottle opening 10 is formed on the bottle body 1, the bottle cap 2 is capable of being opened and closed at the bottle opening 10, a bottle rim 11 is further fixedly disposed outside the bottle body 1, an extending sealing member 20 is disposed on the bottle cap 2, an air hole 21 penetrating through the extending sealing member 20 is disposed on the extending sealing member 20, the extending sealing member 20 is capable of being restored in a first direction and a second direction under external force, and the air hole 21 is configured to be closed when the bottle cap 2 is screwed and opened when the bottle cap 2 is half screwed.

It can be understood that the bottle cap 2 and the bottle body 1 are configured to be opened and closed, and the bottle cap 2 and the bottle body 1 are coupled and matched by a threaded connection, and the bottle cap 2 can be opened and closed by screwing the bottle cap 2 in a clockwise or counterclockwise direction, for example, when the bottle cap 2 is screwed, the bottle cap 2 and the bottle body 1 have at least three states: screwing cover, half screwing (half screwing can also be called half opening and half sealing) and opening; when the bottle cap 2 is screwed and covered on the bottle body 1, the bottle cap 2 cannot be continuously screwed in the screwing direction, when the bottle cap 2 can be opened, the threaded connection relationship between the bottle cap 2 and the bottle body 1 is released, the thread structures of the bottle cap and the bottle body are mutually separated, the semi-screwing state is a state between two extreme states of screwing and covering and being openable, and in the state, the bottle cap 2 can be continuously screwed in the screwing or loosening direction.

In the present embodiment, the bottle cap 2 and the bottle body 1 are configured to: when the bottle cap 2 is closed at the bottle opening 10 along the first direction, the extension sealing part 20 can be abutted against the bottle rim 11, and the bottle cap 2 is configured to be in a full-open state, a semi-sealed state and a full-sealed state;

when the bottle cap 2 is in a fully opened state, a first area on the surface of the extension sealing part 20 is in contact with the bottle rim 11, a cavity inside the bottle body 1 can be communicated with the outside through a gap between the bottle body 1 and the bottle cap 2 and the air hole 21, and the air hole 21 has a first aperture;

when the bottle cap 2 is in a semi-sealed state, the second area on the surface of the extension sealing member 20 is in close contact with the bottle rim 11, the cavity inside the bottle body 1 can be communicated with the outside through the gap between the bottle body 1 and the bottle cap 2 and the air vent 21, and the air vent 21 has a second aperture which is smaller than the first aperture, that is, it can be understood that the air vent 21 is compressed at this time or the amount of compression of the air vent 21 is greater than that of the bottle cap 2 in a fully opened state at this time;

when the bottle cap 2 is in a fully sealed state, the third area on the surface of the extension sealing member 20 is in close contact with the bottle rim 11, and the cavity inside the bottle body 1 is isolated from the outside, wherein the vent 21 is located between the third area and the first area/the second area, or the vent 21 is located in the third area, it can be understood that the vent 21 may be pressed to make at least a partial aperture in the vent 0, or the end of the vent 21 is blocked, or the area where the vent 21 is located is pressed to be driven away from the area capable of communicating with the cavity inside the bottle body 1.

In this embodiment, at least the portion of the extension sealing member 20 close to the bottle rim 11 is inclined or curved in a direction away from the bottle body 1; and/or, one end of the extension sealing part 20 close to the bottle rim 11 is provided with an arc-shaped guide contact surface, and the first area is positioned on the guide contact surface; it is understood that, to achieve the above effect, the airing hole 21 is provided at a portion where the extension sealing member 20 can be inclined or bent in contact with the rim 11, or the rim 11 is provided on the arc-shaped guide contact surface.

In this embodiment, the extension sealing part 20 is an annular member provided at the peripheral edge of the bottle cap 2, or the extension sealing part 20 is formed by extending the peripheral edge of the bottle cap 2; at least a portion of the extension sealing member 20 close to the bottle rim 11 is a flexible member, and it should be noted that the extension sealing member 20 is not only elastically deformable but also recoverable, and for example, the extension sealing member 20 may be a flexible plastic member having high elasticity.

In the present embodiment, the bottle rim 11 is an annular member fixedly disposed at the outer peripheral edge of the body 1, but the body 1, the cap 2, the extension sealing member 20 and the bottle rim 11 may be coaxially disposed.

In the embodiment, a sealing ring 22 is further disposed inside the bottle cap 2, and when the bottle cap 2 is closed at the bottle mouth 10 and is configured in a fully sealed state, the sealing ring 22 can be in sealing contact with the bottle mouth 10; it can be understood that when the bottle cap 2 and the bottle body 1 are in the half-sealing state and the full-opening state, the sealing ring 22 and the bottle opening 10 are unsealed, i.e. conducted.

In this embodiment, the sealing ring 22 has a first contact surface 220, the bottle mouth 10 of the bottle body 1 has a second contact surface 24, and when the bottle cap 2 is in a fully sealed state, the first contact surface 220 can be in sealing contact with the second contact surface 24.

In this embodiment, the first contact surface 220 and the second contact surface 24 are both inclined surfaces that are obliquely arranged, and it is understood that the sealing ring 22 may be an annular member that is circumferentially arranged inside the bottle cap 2, and may also be a continuous planar member, but at least a part of the sealing ring 22 is distributed in an annular area where the bottle cap 2 contacts the bottle mouth 10, for example, a part of the sealing ring 22 extends along a top surface of the bottle cap, and another part extends along a side wall of the bottle cap, that is, the sealing ring 22 has a corner structure to adapt to the structure of the bottle mouth, that is, the first contact surface 220 may be a continuous plane, a curved surface, or a combination of a plurality of continuous surfaces with corners; the second contact surface 24 may also be a continuous arc surface (inclined arc surface), the second contact surface 24 may be inclined from the top end surface of the bottle mouth to the outer wall surface, and the second contact surface 24 may be continuous, smooth and flat, so that a good sealing contact can be formed between the first contact surface 220 and the second contact surface 24.

In this embodiment, the first contact surface 220 includes a plurality of arc-shaped structures 230 disposed at intervals, and a slot structure 231 is formed between two adjacent arc-shaped structures 230, wherein the arc-shaped structures 230 are elastic and can be deformed to be restored under external force, and after the arc-shaped structures 230 are pressed, the deformed portions of the arc-shaped structures 230 pressed can extend into the slot structure 231 to completely close the slot structure 231.

In the present embodiment, the arc-shaped structure 230 has an arc-shaped contact surface capable of sealing and fitting with the second contact surface 24, and it is understood that the arc-shaped structure 230 is a flexible member, which may be selected from materials known to those skilled in the art to have elasticity and flexibility per se, and the specific curvature radius and the surface structure of the arc-shaped contact surface involved are adapted to the second contact surface, and thus no specific surface structure is defined.

In this embodiment, the bottle cap 2 is connected to the bottle body 1 by a threaded connection, an internal thread structure for connecting to the bottle body 1 is provided at a first position on the bottle cap 2, a second position is provided at a joint of the extension sealing member 20 and the bottle cap 2, and an external thread structure is provided on the bottle body 1;

when the bottle cap 2 is in the fully open state, a vertical distance between the first position and the bottle rim 11 is H, a vertical distance between the second position and the bottle rim 11 is H1, a gap is formed between the first contact surface 220 and the second contact surface 24, a vertical distance between the first contact surface 220 and the second contact surface 24 is K1, and H1 is 0.5H and 2K 1;

when the bottle cap 2 is in a semi-sealed state, the extending distance B2 of the extending sealing member 20 on the bottle rim 11 is 0.5B, the vertical distance H2 between the second position and the bottle rim 11 is 0.25H, the arc-shaped structure 230 of the first contact surface 220 abuts against the second contact surface 24, and an air flow channel for air flow is formed between the groove structure 231 and the second contact surface 24;

when the bottle cap 2 is in the fully sealed state, the extending distance B3 of the extending sealing member 20 on the bottle rim 11 is 0.8B, the vertical distance H3 between the second position and the bottle rim 11 is 0.2H, the arc-shaped structure 230 of the first contact surface 220 is in sealing contact with the second contact surface 24, and the groove structure 231 is completely filled with the arc-shaped structure 230, where B is the length of the bottle rim 11 in the second direction.

In this embodiment, the first direction is an axial direction of the body 1 and the cap 2, and the second direction is a radial direction of the body 1 and the cap 2.

It should be noted that the first position is located on the inner wall of the bottle cap 2, when the bottle cap 2 is determined, the internal thread structure on the inner wall of the bottle cap 2 is also determined, the first position is the position where the internal thread structure is located, of course, the first position may be the position where the thread at the topmost end of the internal thread structure is located, or the position where the thread at the lowest end is located, or of course, the position where the thread at another specified position is located, specifically, the specified position of the internal thread structure at which position is used as the first position may be specifically selected as needed, and in order to position the first position, an identifier corresponding to the first position may be provided at a position corresponding to the first position outside the bottle cap 2.

In this embodiment, in order to facilitate the determination and adjustment of the fully open state, the semi-sealed state and the fully sealed state, the bottle body 1 is further provided with a plurality of adjustment marks, and the plurality of adjustment marks respectively correspond to the fully open state, the semi-sealed state and the fully sealed state.

In this embodiment, by labeling three states on the bottle body 1, precise adjustment and switching of multiple states can be achieved, when the bottle cap 2 is in the fully open state, the extending sealing member 20 abuts against the bottle rim 11, a vertical distance between a first position on the bottle cap 2 and the bottle rim 11 is H, a vertical distance between a second position on the bottle cap 2 and the bottle rim 11 is H1, a gap is formed between the first contact surface 220 and the second contact surface 24, a vertical distance between the first contact surface 220 and the second contact surface 24 is K1, and H1 is 0.5H 2K1, by adopting the above structure, a ventilation effect in the fully open state is ensured, and interaction of air flows inside and outside the bottle cap is facilitated, so that an air pressure difference is formed;

In this embodiment, three position lines are marked on the bottle body 1, and the three position lines correspond to the fully open state, the semi-sealed state and the fully sealed state respectively.

The invention is further arranged that when the bottle cap 2 is in a fully open state, the extension sealing part 20 is abutted against the bottle rim 11, the vertical distance between the first position on the bottle cap 2 and the bottle rim 11 is H, the vertical distance between the second position on the bottle cap 2 and the bottle rim 11 is H1, the distance between the first contact surface and the second contact surface 24 is K1, and H1 is 2K 1.

The invention is further arranged that when the bottle cap 2 is in a semi-sealing state, the distance between the first contact surface 220 and the second contact surface 24 is K2, and by adopting the structure arrangement, when the arc-shaped structure 230 is abutted against the second contact surface 24, an airflow channel is formed between the groove structure 231 and the second contact surface 24, and when the semi-sealing state is ensured, the airflow circulation quantity is controlled, so that a good practical effect is realized, a good semi-sealing effect is convenient to form, the height of the thread structure of the bottle cap 2 is H, and the vertical distance between the second position on the bottle cap 2 and the bottle edge 11 is H2; h2 is 1/4H, can extend the height of sealing member 20 when being in half sealed state through control, has improved the inseparable degree of cooperation between bottle lid 2 and the body 1, and the wholeness is strong, also makes when extending sealing member 20 atress, forms stronger elastic support effect, and stability is strong, simple structure.

The invention further provides that the arc-shaped structure 230 is configured such that when the closure 2 is locked, the arc-shaped structure 230 is forced to deform and completely fit with the second contact surface 24, and the distance between the first contact surface 220 and the second contact surface 24 is K3; the height of 2 threaded portions of bottle lid is H, the vertical distance between second position on the bottle lid 2 and bottle edge 11 is H3, and H3 is 1/5H, adopts above-mentioned structural arrangement, and the elasticity that extends sealing member 20 is stronger great, and the reaction force of its formation for form stronger interlock effect between bottle lid 2 and the body 1, stability obtains promoting.

The invention is further arranged to ensure the integrity between the bottle cap 2 and the bottle body 1 and improve the integration degree between the bottle cap 2 and the bottle body 1 by controlling the bending extension length of the extension sealing part 20 when stressed, has strong practicability and simple structure, ensures that the stress points of the bottle cap 2 are concentrated, improves the stress stability and forms stronger stability when in use.

In view of the working environment of the cell culture bottle according to the embodiment of the present invention, the bottle cap, the bottle body, the seal ring, the extension seal member, and the like according to the embodiment of the present invention are made of corrosion-resistant materials.

The specific use method and principle of the cell culture bottle will be described as follows by taking the example that the bottle cap and the bottle body of the cell culture bottle are adjusted from the fully open state to the fully sealed state in combination with the attached drawings:

referring to fig. 1 and 2, the bottle cap 2 is screwed until it is completely loosened, and the bottle cap 2 and the bottle body 1 are in a completely opened state, a first region of the surface of the extended sealing member 20 (which may be, for example, an end surface of the extended sealing member 20) is in contact with the rim 11, while the extended sealing member 20 is in contact with the rim 11, but the extended sealing member 20 is not stressed or has only a small pressure in the first direction, the pressure is not sufficient to resist the elastic force of the extension sealing member 20 itself, the airing hole 21 is in an opened state, and the airing hole 21 may have a first aperture, at the bottle opening 10, the edge of the bottle opening 10 is not contacted with the bottle cap 2 and the sealing ring 22 on the inner side of the bottle cap 2, a gap is formed between the bottle cap 2 and the bottle opening 10 and between the bottle body 1 and the bottle cap 2, and a cavity in the bottle body 1 can be communicated with the outside through the gap between the bottle body 1 and the bottle cap 2 and the air holes 21;

referring to fig. 3 and 4, the bottle cap 2 is screwed in a predetermined direction, during screwing, the bottle cap 2 moves downward (i.e. in a direction close to the bottle body 1) relative to the bottle body 1, the extension sealing member 20 is pressed in the first direction to deform, so as to bend in the second direction, and the end surface of the extension sealing member 20 gradually gets away from the bottle body 1, and the extension sealing member 20 is always kept in close contact with the bottle rim 11 during the pressing and bending process, the contact area between the extension sealing member 20 and the bottle rim 11 is gradually changed from the first area to the second area, at this time, as the extension sealing member 20 is pressed in the first direction, the vent 21 in the extension sealing member 20 is also pressed, so that the aperture is reduced from the first aperture to the second aperture, but the vent 21 is still in a conductive state;

at this time, the sealing ring 22 located inside the bottle cap 2 also moves along the first direction to start to contact with the outer edge of the bottle mouth 10, but only the arc-shaped structure 230 on the first contact surface 220 of the sealing ring 22 contacts with the second contact surface 24 on the outer edge of the bottle mouth 10 at this time, the arc-shaped structure 230 is not deformed, or the arc-shaped structure 230 is deformed by the compression in the first direction, a deformed part of the arc-shaped structure 230, which is partially compressed, extends into the groove structure 231 located between adjacent arc-shaped structures 230, but the groove structure 231 is not completely blocked, so the groove structure 231 is still in a conducting state, and at this time, the bottle cap 2 and the bottle body 1 are configured to be in a semi-sealing or semi-opening state;

referring to fig. 5 and 6, the bottle cap 2 is continuously screwed in the predetermined direction until the bottle cap is completely screwed, during the screwing process, the bottle cap 2 moves downwards (i.e. in a direction close to the bottle body 1) relative to the bottle body 1, the extension sealing member 20 is pressed in the first direction to deform, so as to bend in the second direction, and the end surface of the extension sealing member 20 gradually gets away from the bottle body 1, and keeps close contact with the bottle rim 11 all the time during the pressing and bending process of the extension sealing member 20, the contact area between the extension sealing member 20 and the bottle rim 11 gradually changes from the second area to a third area, at this time, as the extension sealing member 20 is pressed in the first direction, one end of the vent 21 in the extension sealing member 20 is sealed and closed with the bottle rim 11, or the portion provided with the vent 21 is located on the side of the extension sealing member 20, which is opposite to the contact position of the bottle rim 11, away from the bottle body, at the moment, the bottle cap 2 is completely matched with the bottle edge 11 in a sealing way, and the bottle cap 2 blocks the communication between the external environment and the inside of the bottle cap 2;

at this time, the sealing ring 22 located inside the bottle cap 2 also continues to move in the first direction, the arc-shaped structures 230 located on the first contact surface 220 of the sealing ring 22 continue to be pressed and deformed, the volumes of the deformed parts of the arc-shaped structures 230 are increased, and the deformed parts of two adjacent arc-shaped structures 230 extend into the groove structures 231 located between the adjacent arc-shaped structures 230 and completely close the groove structures 231, at this time, the bottle cap 2 and the bottle body 1 are configured to be in a completely sealed state.

It should be noted that the thread structures on the bottle cap 2 and the bottle body 1 are engaged with each other, but the thread structures of the bottle cap 2 and the bottle body 1 are not sealed after being engaged with each other, so that the external environment can be communicated with the cavity inside the bottle body 1 through the gap between the bottle cap 2 and the bottle body 1.

According to the cell culture bottle provided by the embodiment of the invention, the extension sealing component, the air vent, the bottle edge, the sealing ring, the arc-shaped structure, the groove structure and the like are jointly configured into the air vent adjusting structure and the inner sealing structure which are positioned between the bottle body and the bottle cap, so that the sealing and the opening between the bottle body and the bottle cap are realized.

According to the cell culture bottle provided by the embodiment of the invention, the bottle body comprises the bottle opening and the bottle edge, the height of the bottle edge is lower than that of the bottle opening, the ventilation adjusting structure is arranged between the bottle cover and the bottle edge, the full-sealing state and the half-sealing state between the bottle body and the bottle cover can be adjusted through the arranged ventilation adjusting structure, the adjustment of various different airflow circulation states of a single structure is realized, the practicability is higher, meanwhile, an inner sealing structure (such as a sealing ring and the outer edge of the bottle opening) is arranged between the bottle cover and the bottle opening, a double sealing effect is formed, different airflow circulation change effects are formed, and different use requirements are met.

According to the cell culture bottle provided by the embodiment of the invention, the ventilation adjusting structure is arranged as the extending sealing part arranged along the periphery of the bottle cap, the ventilation holes are formed in the extending sealing part, so that the adjustment of the ventilation between the inner cavity of the bottle body and the outside is increased, and the ventilation holes are configured to be closed when the bottle cap is screwed; the bottle cap is opened when being half screwed, two different air capacities are convenient to form, a single structure is realized, and different air ventilation modes are provided.

According to the cell culture bottle provided by the embodiment of the invention, the ventilation adjusting structure is arranged to comprise the ventilation holes arranged on the extending sealing part, and the ventilation holes are communicated with the bottle mouth when not stressed or stressed slightly; when the extension sealing part is stressed by a specified force, the air holes are stressed to be closed, and the air holes are formed in the extension sealing part which is made of flexible materials, so that when the extension sealing part is stressed to be bent, the air holes are closed or semi-closed.

In order to further form different ventilation effects and realize different ventilation requirements, the cell culture bottle provided by the embodiment of the invention further arranges the inner sealing structure to comprise an inner sealing ring arranged in the bottle cap, a first contact surface arranged on the inner sealing ring and inclined, a second contact surface arranged on the bottle mouth, an arc-shaped structure arranged on the first contact surface and a groove structure, and due to the arrangement of the groove structure, three different states are formed between the arc-shaped structure on the first contact surface and the second contact surface, wherein the arc-shaped structure and the second contact surface have larger intervals; the arc-shaped structure is just abutted against the second contact surface, and the groove structure and the second contact surface form a gap airflow channel; the arc-shaped structure is deformed under stress, so that the arc-shaped structure and the second contact surface are completely attached, the three structures have different air capacities and are gradually reduced, and different requirements can be met.

According to the cell culture bottle provided by the embodiment of the invention, when the ventilation adjusting structure and the inner sealing structure are matched, the sealing effect is better, the change of various ventilation amounts is realized, the cell culture bottle is suitable for different requirements, the practicability is high, and the popularization is easy.

According to the cell culture bottle provided by the embodiment of the invention, the ventilation adjusting structure and the inner sealing structure are arranged between the bottle body and the bottle cap, so that the required ventilation quantity is convenient to adjust, the structure is simple, the practicability is high, the requirements of various different ventilation quantities can be met by only one cell culture bottle, the product utilization rate is improved, the popularization is easy, in addition, the cell culture bottle provided by the embodiment of the invention has good identification degree through simple marking, and the practicability is higher.

It should be understood that the above-mentioned embodiments are merely illustrative of the technical concepts and features of the present invention, which are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and therefore, the protection scope of the present invention is not limited thereby. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

1. A cell culture bottle comprises a bottle body (1) and a bottle cap (2), wherein a bottle opening (10) is formed in the bottle body (1), the bottle cap (2) can be opened and closed at the bottle opening (10), and the cell culture bottle is characterized in that:

the bottle comprises a bottle body (1) and is characterized in that a bottle edge (11) is fixedly arranged outside the bottle body (1), an extension sealing part (20) is arranged on the bottle cap (2), an air hole (21) penetrating through the extension sealing part (20) is formed in the extension sealing part (20), the extension sealing part (20) can generate recoverable deformation along a first direction and a second direction under the action of external force, and when the bottle cap (2) is covered on a bottle opening (10) along the first direction, the extension sealing part (20) can be abutted against the bottle edge (11) and enables the bottle cap (2) to be configured into a fully-opened state, a semi-sealed state and a fully-sealed state;

when the bottle cap (2) is in a fully opened state, a first area on the surface of the extension sealing part (20) is in contact with the bottle edge (11), a cavity inside the bottle body (1) can be communicated with the outside through a gap between the bottle body (1) and the bottle cap (2) and the air vent (21), and the air vent (21) has a first aperture;

when the bottle cap (2) is in a semi-sealing state, a second area on the surface of the extension sealing component (20) is in close contact with the bottle edge (11), a cavity inside the bottle body (1) can be communicated with the outside through a gap between the bottle body (1) and the bottle cap (2) and the air vent (21), and the air vent (21) has a second aperture which is smaller than the first aperture;

when the bottle cap (2) is in a fully sealed state, a third area on the surface of the extension sealing component (20) is in close contact with the bottle edge (11), a cavity inside the bottle body (1) is isolated from the outside, wherein the air hole (21) is positioned between the third area and the first area/the second area, or the air hole (21) is positioned in the third area.

2. The cell culture flask according to claim 1, wherein: at least the part of the extension sealing part (20) close to the bottle edge (11) is inclined or bent in the direction far away from the bottle body (1); and/or one end of the extension sealing component (20) close to the bottle edge (11) is provided with an arc-shaped guide contact surface, and the first area is positioned on the guide contact surface.

3. A cell culture flask according to claim 1 or 2, wherein: the extension sealing part (20) is an annular component arranged at the peripheral edge of the bottle cap (2), or the extension sealing part (20) is formed by extending the peripheral edge of the bottle cap (2);

and/or at least the part of the extension sealing part (20) close to the bottle edge (11) is a flexible component.

4. A cell culture flask according to claim 1 or 2, wherein: the bottle rim (11) is an annular component fixedly arranged at the outer peripheral edge part of the bottle body (1);

and/or the bottle body (1), the bottle cap (2), the extension sealing part (20) and the bottle edge (11) are coaxially arranged.

5. The cell culture flask according to claim 1, wherein: the bottle cap (2) is further internally provided with a sealing ring (22), and when the bottle cap (2) is covered on the bottle mouth (10) and is configured to be in a fully-sealed state, the sealing ring (22) can be in sealing contact with the bottle mouth (10).

6. The cell culture flask according to claim 5, wherein: the sealing ring (22) is provided with a first contact surface (220), the bottle opening (10) of the bottle body (1) is provided with a second contact surface (24), and when the bottle cap (2) is in a full-sealing state, the first contact surface (220) can be in sealing fit with the second contact surface (24);

preferably, the first contact surface (220) and the second contact surface (24) are inclined surfaces which are obliquely arranged.

7. The cell culture flask of claim 6, wherein: the first contact surface (220) comprises a plurality of arc-shaped structures (230) arranged at intervals, and a groove structure (231) is formed between every two adjacent arc-shaped structures (230), wherein the arc-shaped structures (230) can be deformed in a restorable manner under the external force, and after the arc-shaped structures (230) are pressed, the deformed parts of the arc-shaped structures (230) generated by pressing can extend into the groove structures (231) to completely close the groove structures (231);

preferably, the arc-shaped structure (230) is provided with an arc-shaped contact surface which can be in sealing fit with the second contact surface (24);

preferably, the arc-like structure (230) is a flexible member.

8. The cell culture flask of claim 7, wherein: the bottle cap (2) is connected with the bottle body (1) in a threaded connection mode, an internal thread structure used for being connected with the bottle body (1) is arranged at a first position on the bottle cap (2), and a joint of the extension sealing part (20) and the bottle cap (2) is provided with a second position;

when the bottle cap (2) is in a fully opened state, the vertical distance between the first position and the bottle edge (11) is H, the vertical distance between the second position and the bottle edge (11) is H1, a gap is formed between the first contact surface (220) and the second contact surface (24), the vertical distance between the first contact surface (220) and the second contact surface (24) is K1, and H1 is 0.5H and 2K 1;

when the bottle cap (2) is in a semi-sealing state, the extension distance B2 of the extension sealing part (20) on the bottle edge (11) is 0.5B, the vertical distance H2 between the second position and the bottle edge (11) is 0.25H, the arc-shaped structure (230) of the first contact surface (220) is abutted against the second contact surface (24), and an air flow channel for air flow is formed between the groove structure (231) and the second contact surface (24);

when the bottle cap (2) is in a fully sealed state, the extension distance B3 of the extension sealing part (20) on the bottle edge (11) is 0.8B, the vertical distance H3 between the second position and the bottle edge (11) is 0.2H, the arc-shaped structure (230) of the first contact surface (220) is in sealing contact with the second contact surface (24), and the groove structure (231) is completely filled by the arc-shaped structure (230), wherein B is the length of the bottle edge (11) in the second direction.

9. The cell culture flask according to claim 1, wherein: the first direction is the axial direction of body (1) and bottle lid (2), the second direction is the radial direction of body (1) and bottle lid (2).

10. The cell culture flask according to claim 1, wherein: the bottle body (1) is also provided with a plurality of adjusting scribed lines which respectively correspond to a full-open state, a half-seal state and a full-seal state;

and/or the bottle cap (2) is a transparent component.