CN113930325B - Automatic microorganism coating device and method - Google Patents
Automatic microorganism coating device and method Download PDFInfo
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- CN113930325B CN113930325B CN202111289704.1A CN202111289704A CN113930325B CN 113930325 B CN113930325 B CN 113930325B CN 202111289704 A CN202111289704 A CN 202111289704A CN 113930325 B CN113930325 B CN 113930325B
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- 238000000576 coating method Methods 0.000 title claims abstract description 152
- 239000011248 coating agent Substances 0.000 title claims abstract description 149
- 238000000034 method Methods 0.000 title claims abstract description 16
- 244000005700 microbiome Species 0.000 title description 12
- 230000000813 microbial effect Effects 0.000 claims abstract description 19
- 230000007246 mechanism Effects 0.000 claims description 42
- 239000007788 liquid Substances 0.000 claims description 17
- 230000001680 brushing effect Effects 0.000 claims description 16
- 238000004140 cleaning Methods 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 8
- 238000005452 bending Methods 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 229910052736 halogen Inorganic materials 0.000 claims description 4
- 150000002367 halogens Chemical class 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000005002 finish coating Substances 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 238000005201 scrubbing Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 230000004907 flux Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 125000003158 alcohol group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000013515 script Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B1/00—Cleaning by methods involving the use of tools
- B08B1/10—Cleaning by methods involving the use of tools characterised by the type of cleaning tool
- B08B1/14—Wipes; Absorbent members, e.g. swabs or sponges
- B08B1/143—Wipes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B1/00—Cleaning by methods involving the use of tools
- B08B1/30—Cleaning by methods involving the use of tools by movement of cleaning members over a surface
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
The invention belongs to the technical field of microbial coating, and discloses a device and a method for coating mobile microbes; the apparatus, comprising: coating rods; the coating rod comprises a coating rod and a sleeve which are matched with each other; the sleeve comprises a sleeve main body and a clamping part which are connected with each other; the clamping part is arranged at the upper end of the sleeve main body; the diameter of the clamping part is larger than that of the sleeve main body; a first through hole and a second through hole which are communicated are formed in the sleeve; the diameter of the first through hole is smaller than that of the second through hole; the coating rod comprises a cylindrical coating rod main body, a coating head is mounted at the lower end of the coating rod main body, a guide rod is arranged at the upper end of the coating rod main body, and the upper end of the guide rod penetrates through the first through hole and extends into the second through hole; the upper end of the guide rod is a threaded section; the threaded section is in threaded connection with a nut, and the nut is located in the second through hole. The invention can well replace the traditional manual coating and solve the problems of small quantity of monoclonals and time consumption in the microbial coating process in the prior art.
Description
Technical Field
The invention belongs to the technical field of microbial coating, and particularly relates to an automatic microbial coating device and method.
Background
The method for obtaining the monoclonal colony by the plating operation of the microorganism sample through the solid agar culture medium is a key process in the microorganism research. The traditional automatic plate coating needs to be realized through steps of liquid transfer, coating and the like, and at present, technicians mainly hold a coating rod to move back and forth for coating, so that the standardization level is low; if a large number of samples need to be applied, it is time consuming and the technician is fatigued, often with mistakes.
At present, the cloning and coating steps in a laboratory are mainly manually operated by an experimental technician; some automatic selection cloner for colony coating of square single-hole plate is also appeared on the market, but the equipment is very expensive, and only 200 samples can be coated in 1 hour, and the coating efficiency is to be improved.
Because the microbial coating often becomes the bottleneck of the experimental flux in the relevant molecular biology experimental process, a device and a process for realizing the automatic coating of the microbes are urgently needed, the experimental flux and the standardization level are greatly improved, and the error rate is reduced.
Disclosure of Invention
The invention aims to provide a device and a method for coating dynamic microorganisms, which aim to solve the technical problems of low efficiency and high error rate of the existing microorganism coating technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an apparatus for automated microbial coating, comprising: coating rods; the coating rod comprises a coating rod and a sleeve which are matched with each other;
the sleeve comprises a sleeve main body and a clamping part which are connected with each other; the clamping part is arranged at the upper end of the sleeve main body; the diameter of the clamping part is larger than that of the sleeve main body; a first through hole and a second through hole which are communicated are formed in the sleeve; the second through hole is positioned at the upper part of the sleeve, the first through hole is positioned at the lower part of the sleeve, and the diameter of the first through hole is smaller than that of the second through hole;
the coating rod comprises a cylindrical coating rod main body, a coating head is mounted at the lower end of the coating rod main body, a guide rod is arranged at the upper end of the coating rod main body, and the upper end of the guide rod penetrates through the first through hole and extends into the second through hole; the upper end of the guide rod is a threaded section; the threaded section is in threaded connection with a nut, and the nut is positioned in the second through hole; the diameter of the nut is larger than that of the first through hole and smaller than that of the second through hole.
The invention further improves the following steps: the length of guide bar is greater than the length of first through-hole.
The invention further improves the following steps: the sleeve main body and the clamping part are both cylindrical, and the clamping part is concentrically arranged at the upper end of the sleeve main body; the diameter of the clamping part is larger than that of the sleeve main body.
The invention further improves the following steps: also comprises a carrier; the carrier comprises a carrier main body, wherein the carrier main body comprises a flat plate positioned at the top and vertical plates supported at two sides of the flat plate; the flat plate is provided with a plurality of third through holes for placing coating rods; the diameter of the third through hole is larger than that of the sleeve and smaller than that of the clamping part.
The invention further improves the following steps: also includes a coating rod cleaning system; the coating rod cleaning system comprises a transmission mechanism and a brushing mechanism;
the transmission mechanism comprises a fixed frame and a direct current motor arranged on the fixed frame; a rotating shaft of the direct current motor extends out of one side of the fixed frame and is fixedly connected with a rotating disc;
the brushing mechanism comprises a brush, an alcohol box, a linear slide rail and a slide block; the hairbrush is arranged in the alcohol box, a sliding block is arranged on a bottom plate of the alcohol box, and the sliding block is arranged on the corresponding linear sliding rail; one side of the bottom plate is provided with a connecting rod linking shaft; one end of the connecting rod is eccentrically hinged with the rotary table, and the other end of the connecting rod is hinged with the connecting rod connecting shaft.
The invention further improves the following steps: the turntable and the connecting rod form a crank-connecting rod mechanism; the alcohol box can reciprocate along the direction of the linear slide rail under the driving of the rotary disc and the connecting rod.
The invention further improves the following steps: the fixed frame comprises two vertical fixed plates which are arranged in parallel; the top of the fixed plate is fixedly connected with a motor base; the motor base is flat; a stepping motor is arranged on the lower surface of the motor base; a rotating shaft of the stepping motor extends out of the motor base and is fixedly connected with a baffle plate; the baffle is provided with a bending induction part;
a first sensor and a second sensor are respectively fixed on the upper surface of the motor base at two sides of the rotating shaft; the first sensor and the second sensor are used for sensing the position of the baffle plate by matching with the bending sensing part.
The invention further improves the following steps: the coating rods are placed on the carrier, and the carrier is fixed above the station of the brushing mechanism through a limiting block; the baffle is positioned above the station of the brushing mechanism and used for pressing the coating rod; coating heads of a plurality of coating rods extend into the alcohol box; the brush can clean the coating head when reciprocating.
The invention further improves the following steps: the coating rod cleaning system also comprises a sterilization mechanism; and a halogen lamp for drying and sterilizing the cleaned coating head is arranged in the sterilization mechanism.
In a second aspect, the present invention provides a method of operating an apparatus for automated microbial coating, comprising the steps of:
s1, a mechanical arm of an automatic pipetting workstation sucks bacterial liquid from a source plate to a target plate;
s2, after the suction head is knocked off by the mechanical arm, moving the carrier to load a plurality of clean coating rods, and then moving the carrier to a target plate to perform moving action to finish coating;
s3, after coating is finished, the mechanical arm carries a coating rod and places the coating rod on a carrier; the mechanical arm clamping carrier moves to the brushing mechanism to clean the coating rods, and then the mechanical arm clamping carrier moves to the sterilization mechanism to dry and sterilize the coating rods.
The invention establishes a process from the operation of liquid samples to the automation of microorganism coating by developing, designing and customizing a coating device. Firstly, engineering technicians compile software driving and process scripts, then an 8-channel liquid-transferring mechanical arm loading suction head of an automatic liquid-transferring workstation absorbs a certain amount of bacterial liquid, the liquid is discharged to a square orifice plate or a 6-orifice plate or other culture plates, then a coating rod is still loaded by the 8-channel liquid-transferring mechanical arm to coat a corresponding orifice plate, the coated orifice plate returns to a cleaning mechanism in a coating device after coating is finished, the coating rod is cleaned, and the coated orifice plate enters a sterilization mechanism to be dried at high temperature and sterilized after cleaning is finished, so that preparation is made for next experiments. The automation degree of the whole coating process is high, and errors caused by human factors are reduced.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides an automatic microbial coating device, which is provided with a new coating rod structure; the coating rod comprises a coating rod and a sleeve which are matched with each other; the sleeve comprises a sleeve main body and a clamping part which are connected with each other; the clamping part is arranged at the upper end of the sleeve main body; the diameter of the clamping part is larger than that of the sleeve main body; a first through hole and a second through hole which are communicated are formed in the sleeve; the second through hole is positioned at the upper part of the sleeve, the first through hole is positioned at the lower part of the sleeve, and the diameter of the first through hole is smaller than that of the second through hole; the coating rod comprises a cylindrical coating rod main body, a coating head is mounted at the lower end of the coating rod main body, a guide rod is arranged at the upper end of the coating rod main body, and the upper end of the guide rod penetrates through the first through hole and extends into the second through hole; the upper end of the guide rod is a threaded section; the threaded section is in threaded connection with a nut, and the nut is positioned in the second through hole; the diameter of the nut is larger than that of the first through hole and smaller than that of the second through hole; the coating rods can be clamped in batches through a mechanical arm, and bacterial liquid is coated in batches; compared with the prior art, the invention can well replace the traditional manual coating, solves the problems of small quantity of monoclonals and time-consuming microorganism coating process in the prior art, and has simple integral process flow, convenient actual operation and wide applicability.
Furthermore, the optimal cleaning and sterilizing time can be determined through experiments, the automatic microbial coating can be realized, the flux is high, the operation is convenient, the application range is wide, and the overall cost is low, so that the process related to the invention can completely replace the traditional manual coating, and the construction of a high-flux automatic platform is promoted.
Furthermore, the coating device is designed and customized based on the automatic liquid transfer workstation, firstly a certain amount of bacteria liquid is absorbed by the automatic liquid transfer workstation and transferred to an agar plate needing coating, then the microorganism sample is uniformly dispersed on the surface of the culture medium plate by using the coating device, and finally the coating rod is cleaned and sterilized by using the cleaning device to realize recycling, so that cross contamination is prevented. The device improves the speed and the precision of the microorganism flat plate coating operation, and releases technical personnel from a large amount of repetitive work. The single clone coating flux of the invention is 1 hour, and the treatment of not less than 300 samples can be completed. Compared with the existing commercial instruments, the monoclonal coating flux of the invention is improved by more than 1.5 times. Meanwhile, the device can be integrated with any major brand of automatic liquid workstation for use, has wide application range and does not influence the original functions of the automatic liquid workstation.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are included to illustrate an exemplary embodiment of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the structure of a coating rod according to the present invention;
FIG. 2 is a schematic structural view of a coating rod and carrier of the present invention;
FIG. 3 is a schematic diagram of an apparatus for automated microbial coating according to the present invention;
FIG. 4 is a schematic view of the transmission mechanism;
FIG. 5 is a schematic view of the brushing mechanism;
FIG. 6 is a bottom view of the scrubbing mechanism shown in FIG. 5;
Detailed Description
The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings. It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The following detailed description is exemplary in nature and is intended to provide further details of the invention. Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention.
Example 1
Referring to fig. 1 to 6, the present embodiment discloses an apparatus for automatically coating microorganisms, comprising: a number of coating rods 20, and a coating rod cleaning system.
Referring to FIG. 1, the coating rod 20 includes a coating rod 201 and a sleeve 202 that cooperate with each other. The sleeve 202 includes a sleeve main body 2021 and a gripping part 2022 connected to each other; the sleeve main body 2021 is cylindrical, and the clamp portion 2022 is also cylindrical and concentrically disposed at the upper end of the sleeve main body 2021; the gripper 2022 has a diameter larger than that of the sleeve main body 2021. A first through hole 2024 and a second through hole 2023 which are communicated are formed in the sleeve 202; the second through hole 2023 is located at the upper portion of the sleeve 202, the first through hole 2024 is located at the lower portion of the sleeve 202, and the diameter of the first through hole 2024 is smaller than that of the second through hole 2023.
The coating rod 201 comprises a cylindrical coating rod main body 2010, a coating head 2011 is mounted at the lower end of the coating rod main body 2010, a guide rod 2012 is arranged at the upper end of the coating rod main body 2010, and the upper end of the guide rod 2012 penetrates through the first through hole 2024 and extends into the second through hole 2023; the upper end of the guide rod 2012 is provided with a threaded section 2013; a nut 2014 is threadedly attached to the threaded section 2013 and is located in the second through hole 2023. The diameter of the nut 2014 is larger than the diameter of the first through hole 2024 and smaller than the diameter of the second through hole 2023. The guide bar 2012 is clearance fit with the first through hole 2024. The coating head 2011 shown in fig. 1 is spherical in shape; the shape may be selected depending on the specific medium and plate, and may be, for example, a rod-like shape, a flat-bottom plate, or the like.
Referring to FIG. 2, a plurality of coating rods 20 may be placed on a carrier 21; the carrier 21 includes a carrier body 210, the carrier body 210 including a slab 2100 at the top and risers 2101 supported on either side of the slab 2100; a plurality of third through holes 2102 are formed in the flat plate 2100; the diameter of the third through-hole 2102 is larger than the diameter of the sleeve 202 and smaller than the diameter of the gripper 2022. Several coating rods 20 can be inserted into corresponding third through holes 2102 with the gripper 2022 exposed at the top of the plate 2100 for gripping by the robotic arm of the automated pipetting station. The bottom of the vertical plate 2101 is provided with a large bottom plate 23. A plurality of limiting blocks 13 are arranged on a workbench of the automatic liquid transferring workstation; the large floor 23 at the bottom of the riser 2101 can catch in the stopper 13 to hold the carrier 21 at a certain station.
Referring to FIG. 3, the coating rod cleaning system includes a drive mechanism 101, a brushing mechanism 102, and a sterilization mechanism 103. The transmission mechanism 101, the brush mechanism 102, and the sterilization mechanism 103 are provided on the work plate 100 of the automated pipetting station.
Referring to fig. 4, the transmission mechanism 101 includes a fixed frame, a baffle 1, a stepping motor 6, and a dc motor 7. The fixed frame comprises two vertical fixed plates 1011 which are arranged in parallel; two vertical fixing plates 1011 arranged in parallel are fixed on the working plate 100 in parallel, and the top of the vertical fixing plates is fixedly connected with a motor base 5. The motor base 5 is flat. The lower surface of the motor base 5 is provided with a stepping motor 6; a rotating shaft 2 of the stepping motor 6 extends out of the motor base 5 and is fixedly connected with the baffle plate 1; the baffle 1 is provided with a bending induction part 1012. The upper surface of the motor base 5 is positioned at two sides of the rotating shaft 2 and is respectively fixed with a first sensor 3 and a second sensor 4. The stepping motor 6 can drive the baffle 1 to rotate through the rotating shaft 2, and the position of the baffle 1 can be sensed through the first sensor 3, the second sensor 4 and the bending sensing part 1012. The direct current motor 7 is arranged between the two vertical fixing plates 1011, and a rotating shaft of the direct current motor 7 extends out of one vertical fixing plate 1011 and is fixedly connected with the turntable 8; a connecting rod 9 is eccentrically arranged on the rotary disc 8. The rotation of the direct current motor 7 can drive the connecting rod 9 to reciprocate. The fixed frame is covered with a motor cover 22.
Referring to fig. 5 and 6, the brushing mechanism 102 includes a brush 10, an alcohol box 11, a linear slide rail 16 and a slider 17. The brush 10 is arranged in the alcohol box 11, two sliding blocks 17 are arranged on the lower part of a bottom plate 12 of the alcohol box 11 in parallel, and the two sliding blocks 17 are arranged on two linear sliding rails 16; the linear slide 16 is fixed to the work plate 100. A link shaft 14 is installed at one side of the base plate 12. One end of the connecting rod 9 is hinged with the rotary table 8, and the other end is hinged with the connecting rod connecting shaft 14. A plurality of coating rods 20 are placed on a carrier 21, and the carrier 21 is fixed above a station of the brushing mechanism 102 through a limiting block 13; when the direct current motor 7 rotates, the rotating disc 8 is driven to rotate, and then the connecting rod 9 can drive the alcohol box 11 to perform reciprocating linear motion under the cooperation of the sliding block 17 and the linear sliding rail 16, and further the coating head 2011 of the coating rod 20 is cleaned through the hairbrush 10. Before cleaning, the stepping motor 6 in the transmission mechanism 101 rotates to drive the baffle 1 to rotate through the rotating shaft 2, the position of the baffle 1 is sensed through the matching of the first sensor 3 and the second sensor 4 with the bending sensing part 1012, and the baffle 1 stops rotating when being positioned above a station of the brushing mechanism 102 and pressing the carrier 21; thus, when the brush 10 cleans the coating head 2011 of the coating rod 20, the position of the coating rod 20 can be kept stable, and the cleaning effect is ensured. A sensor seat 18 is also arranged below the station of the brushing mechanism 102, and a third sensor 15 is arranged in the sensor seat 18; the bottom edge of the alcohol box 11 is provided with a trigger piece 19; the trigger piece 19 and the third sensor 15 cooperate with each other to sense the working limit position of the alcohol cartridge 11.
The sterilizing unit 103 is provided with a halogen lamp 24. A halogen lamp 24 fixed to the work plate 100 and capable of heating the cleaned coating rod 20 located above the sterilizing means 103; the drying temperature in the sterilization mechanism 103 is more than 500 ℃ to dry and sterilize the coating rod 20; the dried and sterilized coating rod 20 can be integrally grabbed and moved to a position to be worked by a mechanical arm for standby.
Example 2
The embodiment discloses a working method of an automatic microorganism coating device, which comprises the following steps:
s1, an 8-channel mechanical arm of an automatic pipetting workstation sucks a certain amount of bacteria liquid from a source plate to a target plate;
s2, after the suction head is knocked off by the pipetting mechanical arm with the channels 8, moving the pipette mechanical arm to a carrier 21 to load a clean coating rod 20, and then moving the pipette mechanical arm to a target plate to perform a moving action to finish coating; the coating rod 201 in the coating rod 20 is generally at the lowest working position due to gravity; some culture mediums have fluctuant heights, the coating rod 201 can be extruded and stretched upwards according to the fluctuant heights of the culture mediums, the bacterium liquid can not be damaged, and the accuracy of the experiment is ensured; the coating rod 20 is designed for flexible profiling of different high and low culture media, the coating rod and the sleeve can move relatively, the condition that the solid culture medium in the pore plate is uneven can be adapted, and the solid culture medium moves up and down relatively according to the height of the culture medium, so that the bacterial liquid is uniformly distributed in the pore plate;
s3, after coating is completed, the 8-channel pipetting mechanical arm carries the coating rod to move to the brushing mechanism 102 for cleaning, then the coating rod is dried and sterilized at a high temperature of the sterilization mechanism 103, finally the 8-channel pipetting mechanical arm moves to the carrier frame to unload the coating rod, and then the coating rod is stood and dried.
It will be appreciated by those skilled in the art that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or are equivalent to the scope of the invention are intended to be embraced therein.
Claims (10)
1. An apparatus for automated microbial coating, comprising: a coating rod (20); the coating rod (20) comprises a coating rod (201) and a sleeve (202) which are matched with each other;
the sleeve (202) comprises a sleeve main body (2021) and a gripping part (2022) which are connected with each other; the clamping part is arranged at the upper end of the sleeve main body (2021); the diameter of the clamping part (2022) is larger than that of the sleeve main body (2021); a first through hole (2024) and a second through hole (2023) which are communicated are arranged in the sleeve (202); the second through hole (2023) is positioned at the upper part of the sleeve (202), the first through hole (2024) is positioned at the lower part of the sleeve (202), and the diameter of the first through hole (2024) is smaller than that of the second through hole (2023);
the coating rod (201) comprises a cylindrical coating rod main body (2010), a coating head (2011) is installed at the lower end of the coating rod main body (2010), a guide rod (2012) is arranged at the upper end of the coating rod main body (2010), and the upper end of the guide rod (2012) penetrates through the first through hole (2024) and extends into the second through hole (2023); the upper end of the guide rod (2012) is provided with a threaded section (2013); a nut (2014) is connected to the threaded section (2013) in a threaded mode and is located in the second through hole (2023); the diameter of the nut 2014 is larger than that of the first through hole 2024 and smaller than that of the second through hole 2023.
2. An automated microbial coating apparatus as claimed in claim 1, wherein the length of the guide bar (2012) is greater than the length of the first through hole (2024).
3. The device for automatic microbial coating according to claim 1, wherein the sleeve body (2021) and the clamping unit (2022) are cylindrical, and the clamping unit (2022) is concentrically disposed at the upper end of the sleeve body (2021); the diameter of the gripping part (2022) is larger than that of the sleeve main body (2021).
4. An automated microbial coating apparatus as claimed in claim 1, further comprising a carriage (21); the carrier (21) comprises a carrier body (210), the carrier body (210) comprising a slab (2100) at the top and risers (2101) supported on either side of the slab (2100); the flat plate (2100) is provided with a plurality of third through holes (2102) for placing the coating rods (20); the diameter of the third through hole (2102) is larger than that of the sleeve (202) and smaller than that of the clamping part (2022).
5. The apparatus of claim 1, further comprising a coating rod cleaning system; the coating rod cleaning system comprises a transmission mechanism (101) and a brushing mechanism (102);
the transmission mechanism (101) comprises a fixed frame and a direct current motor (7) arranged on the fixed frame; a rotating shaft of the direct current motor (7) extends out of one side of the fixed frame and is fixedly connected with a rotating disc (8);
the brushing mechanism (102) comprises a brush (10), an alcohol box (11), a linear slide rail (16) and a slide block (17); the brush (10) is arranged in the alcohol box (11), a sliding block (17) is installed on a bottom plate (12) of the alcohol box (11), and the sliding block (17) is installed on the corresponding linear sliding rail (16); one side of the bottom plate (12) is provided with a connecting rod link shaft (14); one end of the connecting rod (9) is eccentrically hinged with the turntable (8), and the other end is hinged with the connecting rod connecting shaft (14).
6. An automated microbial coating apparatus according to claim 5, wherein the turntable (8) and the connecting rod (9) constitute a crank-link mechanism; the alcohol box (11) can reciprocate along the direction of the linear slide rail (16) under the driving of the turntable (8) and the connecting rod (9).
7. An apparatus for automated microbial coating according to claim 5, wherein the fixed frame comprises two parallel vertical fixed plates (1011); the top of the fixing plate (1011) is fixedly connected with a motor base (5); the motor base (5) is flat; a stepping motor (6) is arranged on the lower surface of the motor base (5); a rotating shaft (2) of the stepping motor (6) extends out of the motor base (5) and is fixedly connected with a baffle plate (1); a bending induction part (1012) is arranged on the baffle (1);
a first sensor (3) and a second sensor (4) are respectively fixed on the upper surface of the motor base (5) at two sides of the rotating shaft (2); the first sensor (3) and the second sensor (4) are used for sensing the position of the baffle (1) by matching with the bending sensing part (1012).
8. The device for the automatic microbial coating according to claim 7, wherein a plurality of coating rods (20) are placed on a carrier (21), and the carrier (21) is fixed above a station of the brushing mechanism (102) through a limiting block (13); the baffle (1) is positioned above the station of the brushing mechanism (102) and presses the coating rod (20); coating heads (2011) of a plurality of coating rods (20) extend into the alcohol box (11); the brush (10) is capable of cleaning the coating head (2011) when reciprocating.
9. An apparatus for automated microbial coating according to claim 5, wherein the coating rod cleaning system further comprises a sterilization mechanism (103); the sterilization mechanism (103) is provided with a halogen lamp (24) for drying and sterilizing the cleaned coating head (2011).
10. A method of operating an automated microbial coating apparatus, according to claim 9, comprising the steps of:
s1, a mechanical arm of an automatic liquid transfer workstation sucks bacterial liquid from a source plate to a target plate;
s2, after the suction head is knocked off by the mechanical arm, moving the carrier to load a plurality of clean coating rods (20), and then moving the carrier to a target plate to perform a moving action to finish coating;
s3, after coating is finished, the mechanical arm carries a coating rod and places the coating rod on a carrier; the robot arm holding carrier moves to a scrubbing mechanism (102) to clean the coating rod (20), and then the robot arm holding carrier moves to a sterilizing mechanism (103) to dry and sterilize the coating rod (20).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111289704.1A CN113930325B (en) | 2021-11-02 | 2021-11-02 | Automatic microorganism coating device and method |
| PCT/CN2021/135534 WO2023077598A1 (en) | 2021-11-02 | 2021-12-03 | Apparatus and method for automated microorganism application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111289704.1A CN113930325B (en) | 2021-11-02 | 2021-11-02 | Automatic microorganism coating device and method |
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| CN113930325A CN113930325A (en) | 2022-01-14 |
| CN113930325B true CN113930325B (en) | 2023-01-24 |
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| CN202111289704.1A Active CN113930325B (en) | 2021-11-02 | 2021-11-02 | Automatic microorganism coating device and method |
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| WO (1) | WO2023077598A1 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10309199A (en) * | 1997-05-13 | 1998-11-24 | Yoshikawa Kogyo Co Ltd | Automatic coating of specimen and device |
| CN111073808A (en) * | 2020-03-03 | 2020-04-28 | 上海汇像信息技术有限公司 | Microbial detection culture dish coating device and coating control method |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2668495B1 (en) * | 1990-10-26 | 1993-10-08 | Bernard Lange | CONCEAL OF SAMPLING FOR BACTERIOLOGICAL USE. |
| JP2013000051A (en) * | 2011-06-16 | 2013-01-07 | Medica Tekku Kk | Specimen coater |
| CN203866315U (en) * | 2014-05-12 | 2014-10-08 | 淄博职业学院 | Automatic fungus liquid coating device |
| CN105586254B (en) * | 2016-03-22 | 2017-09-12 | 陈志强 | A kind of microbionation device and its method for carrying out microbionation |
| US10737288B2 (en) * | 2018-02-22 | 2020-08-11 | Bin Hong | Automatic spin coating system |
| CN110499243A (en) * | 2019-08-29 | 2019-11-26 | 贵州大学 | A kind of microorganism spreading rod |
| CN211645238U (en) * | 2020-01-16 | 2020-10-09 | 重庆医科大学附属永川医院 | Microbial inoculating loop |
| CN112195097A (en) * | 2020-10-13 | 2021-01-08 | 上海莫杜生物科技有限公司 | Rotary inoculation mechanism |
-
2021
- 2021-11-02 CN CN202111289704.1A patent/CN113930325B/en active Active
- 2021-12-03 WO PCT/CN2021/135534 patent/WO2023077598A1/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10309199A (en) * | 1997-05-13 | 1998-11-24 | Yoshikawa Kogyo Co Ltd | Automatic coating of specimen and device |
| CN111073808A (en) * | 2020-03-03 | 2020-04-28 | 上海汇像信息技术有限公司 | Microbial detection culture dish coating device and coating control method |
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| CN113930325A (en) | 2022-01-14 |
| WO2023077598A1 (en) | 2023-05-11 |
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