CN113926000A - Preparation method of silk fibroin drug delivery tissue engineering scaffold - Google Patents
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- CN113926000A CN113926000A CN202111226462.1A CN202111226462A CN113926000A CN 113926000 A CN113926000 A CN 113926000A CN 202111226462 A CN202111226462 A CN 202111226462A CN 113926000 A CN113926000 A CN 113926000A
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/28—Formation of filaments, threads, or the like while mixing different spinning solutions or melts during the spinning operation; Spinnerette packs therefor
- D01D5/30—Conjugate filaments; Spinnerette packs therefor
- D01D5/34—Core-skin structure; Spinnerette packs therefor
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Abstract
The invention provides a preparation method of a silk fibroin drug delivery tissue engineering scaffold, belonging to the technical field of drug preparation, wherein a coaxial electrospinning method is adopted to prepare the drug delivery tissue engineering scaffold taking silk fibroin as a sheath and PEO containing a drug as a core material; wherein, the outer layer raw material of the coaxial electrospinning is silk fibroin aqueous solution, and the inner layer raw material is polyethylene oxide (PEO) aqueous solution dispersed with drugs. The tissue engineering scaffold prepared by the invention has high strength, good biocompatibility, biodegradability, high oxygen permeability and light transmittance; the amino acid branched chain on the surface of the silk fibroin can provide an ideal adhesive point position for cell active element, so that the silk material is easy to modify on the surface; the silk-based biomaterial can better adapt to complex in-vivo environments.
Description
Technical Field
The invention relates to the technical field of drug preparation, in particular to a preparation method of a silk fibroin drug delivery tissue engineering scaffold.
Background
In recent years, in the medical field, biomaterials are increasingly used in diagnosis and treatment of diseases and repair and replacement of damaged tissues or organs of the human body. Superior biomaterials can be combined with a dynamic biological environment after implantation to guide the organism to respond as desired through bioactive components.
Tissue engineering technology is an important area of biomaterial application. The damaged tissue may be replaced by or regenerated with the aid of tissue engineering structures. Tissue engineering structures are usually composed of two parts, cell culture and artificial scaffolds. Tissue engineering scaffolds need to mimic the natural extracellular matrix to the greatest extent, provide optimal environmental signals for cells, including chemical, mechanical, and spatial topological signals, and not only provide physical support for cells, but also influence cell phenotype through environmental signals during cell propagation. Therefore, excellent tissue engineering scaffolds need to have good biocompatibility, permeability to water, oxygen, and other nutrients, appropriate biodegradability and degradation rate (the degradation rate of the regeneration-inducing scaffold needs to be consistent with the tissue regeneration rate).
Disclosure of Invention
The invention aims to provide a preparation method of a silk fibroin drug delivery tissue engineering scaffold, which aims to solve at least one technical problem in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a preparation method of a silk fibroin drug delivery tissue engineering scaffold, which comprises the following steps:
preparing a drug delivery tissue engineering scaffold taking silk fibroin as a sheath and PEO (polyethylene oxide) containing a drug as a core material by adopting a coaxial electrospinning method; wherein, the outer layer raw material of the coaxial electrospinning is silk fibroin aqueous solution, and the inner layer raw material is polyethylene oxide (PEO) aqueous solution dispersed with drugs.
Preferably, the silk fibroin aqueous solution and the PEO aqueous solution are respectively driven by two mechanical injection pumps to enter a coaxial electrospinning nozzle; starting an electric field between the nozzle and the fiber collecting device; and (3) allowing the silk fibroin aqueous solution and the PEO aqueous solution to fall onto a collecting device through an electric field, and collecting by the collecting device to obtain the drug delivery tissue engineering scaffold.
Preferably, the collected fiber cloth is treated by adopting a method of high-pressure steam sterilization, water vapor annealing or organic solvent soaking, the crystallinity of the silk fibroin can be regulated and controlled, and the regulation and control of the degradation time of the drug delivery tissue engineering scaffold are realized.
Preferably, the organic solvent is methanol or ethanol.
Preferably, the preparation of the silk fibroin aqueous solution comprises: boiling and degumming silkworm cocoons, and dissolving the silkworm cocoons in a salt solution; desalting to obtain silk fibroin aqueous solution.
Preferably, the silkworm cocoons are boiled using an aqueous sodium bicarbonate solution.
Preferably, the boiled degummed silkworm cocoons are dissolved in a lithium bromide solution.
Preferably, the coaxial electrospinning nozzle comprises:
a nozzle body;
the nozzle body is provided with a first incident port, and an inner pipe is communicated with the first incident port;
the nozzle body is also provided with a second incident port, and an outer tube is communicated with the second incident port;
the inner pipe and the outer pipe are coaxially arranged, and the inner pipe penetrates through the outer pipe;
the first entrance port and the second entrance port are respectively connected with an injection pump, the inner tube is used for conveying inner layer raw materials, and the outer tube is used for conveying outer layer raw materials.
Preferably, a flat plate is used as the collecting means.
Preferably, the collecting device comprises a rotating shaft, and two ends of the rotating shaft are connected with a rotary driving motor.
The invention has the beneficial effects that: the prepared tissue engineering scaffold has high strength, good biocompatibility, biodegradability, high oxygen permeability and light transmittance; the amino acid branched chain on the surface of the silk fibroin can provide an ideal adhesive point position for cell active element, so that the silk material is easy to modify on the surface; the silk-based biomaterial can better adapt to complex in-vivo environments.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a perspective view of a coaxial electrospinning nozzle according to an embodiment of the present invention.
FIG. 2 is a cross-sectional structural view of a coaxial electrospinning nozzle according to an embodiment of the present invention.
Wherein: 1-a nozzle body; 2-a first entrance port; 3-inner tube; 4-a second entrance port; 5-an outer tube; 6-a transverse channel; 7-containing chamber.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below by way of the drawings are illustrative only and are not to be construed as limiting the invention.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
As used herein, the singular forms "a", "an", "the" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
For the purpose of facilitating an understanding of the present invention, the present invention will be further explained by way of specific embodiments with reference to the accompanying drawings, which are not intended to limit the present invention.
It should be understood by those skilled in the art that the drawings are merely schematic representations of embodiments and that the elements shown in the drawings are not necessarily required to practice the invention.
Example 1
Silk is silk fiber which constitutes silkworm cocoon, and the main components are silk fibroin and sericin. Wherein the silk fibroin is a natural biological macromolecule and is composed of highly repetitive amino acid sequences. These amino acid sequences can be combined into beta-sheet through hydrogen bonds in space, so that silk becomes one of the highest-strength materials in nature. The silk fibroin has the advantages of high strength, good biocompatibility and biodegradability, high oxygen permeability, light transmittance and the like, so that the silk fibroin becomes a popular new biological material. The amino acid branched chain on the surface of the silk fibroin can provide ideal adhesive point positions for cell active elements, so that the silk material is easy to modify on the surface.
The silk-based biomaterial can better adapt to complex in-vivo environments. Compared with biological materials such as collagen, polylactic acid and the like, the fibroin has better biocompatibility and controllable biodegradability, and the degradation time is as short as several seconds and as long as several years, so the fibroin is more suitable for being applied to degradable stent preparations and controllable release drug delivery devices.
The embodiment 1 of the invention provides a preparation method of a silk fibroin drug delivery tissue engineering scaffold, which comprises the following steps:
preparing a drug delivery tissue engineering scaffold taking silk fibroin as a sheath and PEO (polyethylene oxide) containing a drug as a core material by adopting a coaxial electrospinning method; wherein, the outer layer raw material of the coaxial electrospinning is silk fibroin aqueous solution, and the inner layer raw material is polyethylene oxide (PEO) aqueous solution dispersed with drugs.
In this example 1, a silk fibroin aqueous solution and a PEO aqueous solution were respectively driven by two mechanical injection pumps to enter a coaxial electrospinning nozzle; starting an electric field between the nozzle and the fiber collecting device; and (3) allowing the silk fibroin aqueous solution and the PEO aqueous solution to fall onto a collecting device through an electric field, and collecting by the collecting device to obtain the drug delivery tissue engineering scaffold.
In this example 1, the collected fiber cloth is treated by autoclaving, steam annealing or organic solvent soaking, so as to control the crystallinity of silk fibroin and achieve the control of the degradation time of the drug delivery tissue engineering scaffold.
In this example 1, the organic solvent is methanol or ethanol.
In this example 1, the preparation of an aqueous solution of silk fibroin comprises: boiling and degumming silkworm cocoons, and dissolving the silkworm cocoons in a salt solution; desalting to obtain silk fibroin aqueous solution.
The cocoons are boiled with an aqueous sodium bicarbonate solution.
And (3) dissolving the boiled and degummed silkworm cocoons in a lithium bromide solution.
The coaxial electrospinning nozzle described in this example 1 includes:
a nozzle body;
the nozzle body is provided with a first incident port, and an inner pipe is communicated with the first incident port;
the nozzle body is also provided with a second incident port, and an outer tube is communicated with the second incident port;
the inner pipe and the outer pipe are coaxially arranged, and the inner pipe 3 penetrates through the outer pipe;
the first incident port and the second incident port are respectively connected with an injection pump, the injection pump connected with the first incident port is used for injecting inner layer raw materials, the injection pump connected with the second incident port is used for injecting outer layer raw materials, the inner tube is used for conveying the inner layer raw materials, and the outer tube is used for conveying the outer layer raw materials.
In this example 1, a flat plate was used as the collecting means.
Example 2
The embodiment 2 of the invention provides a preparation method of a drug delivery tissue engineering scaffold, which comprises the following steps:
preparing a drug delivery tissue engineering scaffold taking silk fibroin as a sheath and PEO (polyethylene oxide) containing a drug as a core material by adopting a coaxial electrospinning method; wherein, the outer layer raw material of the coaxial electrospinning is silk fibroin aqueous solution, and the inner layer raw material is polyethylene oxide (PEO) aqueous solution dispersed with drugs.
In this example 2, a silk fibroin aqueous solution and a PEO aqueous solution were respectively driven by two mechanical injection pumps to enter a coaxial electrospinning nozzle; starting an electric field between the nozzle and the fiber collecting device; and (3) allowing the silk fibroin aqueous solution and the PEO aqueous solution to fall onto a collecting device through an electric field, and collecting by the collecting device to obtain the drug delivery tissue engineering scaffold.
In this embodiment 2, the collected fiber cloth is treated by high-pressure steam sterilization, steam annealing or organic solvent soaking, so that the crystallinity of the silk fibroin can be regulated and controlled, and the degradation time of the drug delivery tissue engineering scaffold can be regulated and controlled.
In the newly collected fiber cloth, the silk fibroin secondary structure is mainly random coil, contains a small amount of b folds and can be melted when meeting water. Therefore, the fiber cloth must be further treated to increase the crystallinity so that its b-fold content exceeds a threshold value to achieve insolubility. At the same time, an increase in b-fold content also means an increase in the degradation time of the scaffold in vivo. Therefore, after regulating the b-fold content to be higher than the threshold value, the regulation of the biodegradation time can be simultaneously realized.
In this example 2, the organic solvent is methanol or ethanol. When the fiber cloth is contacted with polar organic solvent such as methanol and ethanol, the secondary structure of the silk fibroin is instantly converted from random coil to b-fold. After the solvent is soaked, the silk fibroin is further crystallized in the process of airing the fiber cloth at room temperature. In this process, both solvent concentration and soaking time can affect the final crystallinity.
The high-pressure steam sterilization method is mainly used for integrating crystallization promotion and sterilization into the same step, so that the fiber cloth treatment process is simplified. The high-pressure steam equipment used at present is of a low exhaust type, is at the temperature of 121.3 ℃ for 30 minutes, and has not been measured for cleanliness.
The high-pressure steam sterilization is performed by high-temperature and high-pressure sterilization, not only can kill common microorganisms such as bacteria and fungi, but also has killing effect on spores and spores, and is the most reliable and most common physical sterilization method. The sterilization device is mainly used for sterilizing high-temperature resistant articles, such as culture media, metal instruments, glass, enamel, dressings, rubber and some medicines. Types and styles of autoclave are numerous, such as: firstly, a lower exhaust type pressure steam sterilizer is a commonly used sterilization device, the pressure is increased to 103.4kPa (1.05kg/cm2), the temperature reaches 121.3 ℃, and the sterilization can be maintained for 15-30 minutes, thereby achieving the purpose of sterilization. ② the pulse vacuum pressure steam sterilizer has become the most advanced sterilizing equipment. The sterilization condition requirements are as follows: the steam pressure is 205.8kPa (2.1kg/cm2), the temperature reaches above 132 ℃ and is maintained for 10 minutes, and all microorganisms including spores and spores with tenacious resistance can be killed.
In this example 2, extracting a regenerated silk fibroin aqueous solution from oven-dried cocoons to prepare a silk fibroin aqueous solution includes: boiling the silkworm cocoon with low concentration sodium bicarbonate water solution to degum the silkworm cocoon, dissolving the degummed silkworm cocoon in high concentration lithium bromide water solution, and removing lithium bromide salt through dialysis to obtain regenerated silk fibroin water solution with the highest concentration up to 35% mass fraction.
As shown in fig. 1 to 2, the coaxial electrospinning nozzle of this embodiment 2 includes:
a nozzle body 1;
the nozzle body 1 is provided with a first incident port 2, and an inner tube 3 is communicated with the first incident port 2;
the nozzle body 1 is also provided with a second incident port 4, and an outer tube 5 is communicated with the second incident port 4;
the inner pipe 3 and the outer pipe 5 are coaxially arranged, and the inner pipe 3 penetrates through the outer pipe 5;
In the present embodiment 2, the first incident port 2 is located at the top of the nozzle body 1, and the inner tube 3 is disposed longitudinally along the nozzle body 1. The second entrance port 4 is located the lateral part of nozzle body 1, and the second entrance port 4 holds chamber 7 through 6 intercommunications of transverse channel, holds chamber 7 intercommunication outer tube 5, and the inner tube passes and holds chamber 7 and stretch into outer tube 5, with the coaxial setting of outer tube 5. The syringe pump is injected the inlayer raw materials by first incident port 2 in inner tube 3, the inlayer raw materials flows down along inner tube 3, get into high-voltage electric field until flowing out inner tube 3, another syringe pump is injected the outer raw materials into transverse channel 6 at first by second incident port 4, the inlayer raw materials gets into along transverse channel 6 and holds chamber 7, get into outer tube 5 again by holding chamber 7, along the chamber way downward flow between outer tube 5 and the inner tube 3, until getting into high-voltage electric field, in high-voltage electric field, inlayer raw materials and outer raw materials form the fibre.
In this embodiment 2, the collecting device includes a rotating shaft, and two ends of the rotating shaft are connected to a rotation driving motor. The rotating speed of the driving motor is adjustable, and deposition from disordered fibers to ordered fibers (along the weft direction of the stainless steel shaft) can be realized by adjusting the rotating speed.
In this example 2, the silk fibroin sheath thickness and the diameter of the PEO drug core layer can be controlled by changing the diameter ratio of the inner tube and the outer tube.
The above description is only a preferred embodiment of the present disclosure and is not intended to limit the present disclosure, and various modifications and changes may be made to the present disclosure by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present disclosure should be included in the protection scope of the present disclosure.
Although the present disclosure has been described with reference to the specific embodiments shown in the drawings, it is not intended to limit the scope of the present disclosure, and it should be understood by those skilled in the art that various modifications and variations can be made without inventive faculty based on the technical solutions disclosed in the present disclosure.
Claims (10)
1. A preparation method of a silk fibroin drug delivery tissue engineering scaffold is characterized by comprising the following steps:
preparing a drug delivery tissue engineering scaffold taking silk fibroin as a sheath and PEO (polyethylene oxide) containing a drug as a core material by adopting a coaxial electrospinning method; wherein, the outer layer raw material of the coaxial electrospinning is silk fibroin aqueous solution, and the inner layer raw material is polyethylene oxide (PEO) aqueous solution dispersed with drugs.
2. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 1, wherein the silk fibroin aqueous solution and the PEO aqueous solution are respectively driven into the coaxial electrospinning nozzle by two mechanical injection pumps; starting an electric field between the nozzle and the fiber collecting device; and (3) allowing the silk fibroin aqueous solution and the PEO aqueous solution to fall onto a collecting device through an electric field, and collecting by the collecting device to obtain the drug delivery tissue engineering scaffold.
3. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 1, wherein the collected fiber cloth is treated by high-pressure steam sterilization, steam annealing or organic solvent soaking, so that the crystallinity of the silk fibroin can be regulated and controlled, and the degradation time of the drug delivery tissue engineering scaffold can be regulated and controlled.
4. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 3, wherein the organic solvent is methanol or ethanol.
5. The method of preparing the silk fibroin drug delivery tissue engineering scaffold of claim 1, wherein the preparing the aqueous silk fibroin solution comprises: boiling and degumming silkworm cocoons, and dissolving the silkworm cocoons in a salt solution; desalting to obtain silk fibroin aqueous solution.
6. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 5, wherein silkworm cocoons are boiled with an aqueous solution of sodium bicarbonate.
7. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 5, wherein the boiled degummed silkworm cocoons are dissolved in a lithium bromide solution.
8. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 2, wherein the coaxial electrospinning nozzle comprises:
a nozzle body;
the nozzle body is provided with a first incident port, and an inner pipe is communicated with the first incident port;
the nozzle body is also provided with a second incident port, and an outer tube is communicated with the second incident port;
the inner pipe and the outer pipe are coaxially arranged, and the inner pipe penetrates through the outer pipe;
the first entrance port and the second entrance port are respectively connected with an injection pump, the inner tube is used for conveying inner layer raw materials, and the outer tube is used for conveying outer layer raw materials.
9. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 8, wherein a flat plate is used as a collection device.
10. The method for preparing the silk fibroin drug delivery tissue engineering scaffold according to claim 8, wherein the collection device comprises a rotating shaft, and both ends of the rotating shaft are connected with a rotation driving motor.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488929A (en) * | 2011-12-16 | 2012-06-13 | 东华大学 | Regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor and preparation method thereof |
| CN103893815A (en) * | 2014-03-31 | 2014-07-02 | 浙江大学 | Scald and burn dressing prepared by coaxial electrostatic spinning method and preparation method thereof |
| WO2017137937A1 (en) * | 2016-02-10 | 2017-08-17 | Jawaharlal Nehru Centre For Advanced Scientific Research | A composite, scaffold and applications thereof |
| US20200109491A1 (en) * | 2017-04-04 | 2020-04-09 | Silk Biomaterials S.R.L. | Nanofibers comprising fibroin as well as system comprising hydrogel and said nanofibers |
| CN111676537A (en) * | 2019-03-11 | 2020-09-18 | 西安交通大学 | Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology |
-
2021
- 2021-10-21 CN CN202111226462.1A patent/CN113926000A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102488929A (en) * | 2011-12-16 | 2012-06-13 | 东华大学 | Regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor and preparation method thereof |
| CN103893815A (en) * | 2014-03-31 | 2014-07-02 | 浙江大学 | Scald and burn dressing prepared by coaxial electrostatic spinning method and preparation method thereof |
| WO2017137937A1 (en) * | 2016-02-10 | 2017-08-17 | Jawaharlal Nehru Centre For Advanced Scientific Research | A composite, scaffold and applications thereof |
| US20200109491A1 (en) * | 2017-04-04 | 2020-04-09 | Silk Biomaterials S.R.L. | Nanofibers comprising fibroin as well as system comprising hydrogel and said nanofibers |
| CN111676537A (en) * | 2019-03-11 | 2020-09-18 | 西安交通大学 | Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology |
Non-Patent Citations (1)
| Title |
|---|
| 段久芳: "《天然高分子材料》", 31 March 2016, pages: 291 - 292 * |
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