CN113925021B - Animal sleep rhythm regulation and control test method - Google Patents

Abstract

The application provides an animal sleep rhythm control test method. The animal sleep rhythm regulation test method includes: obtaining basic physiological information of animals; obtaining a scoring model; inputting basic physiological information of animals into the scoring model to obtain animal scores; obtaining an adjustment database, the adjustment database includes at least one sleep rhythm adjustment scheme and at least one A preset score, a sleep rhythm adjustment scheme is associated with at least one preset score; determine whether the acquired animal score is the same as one of the preset scores in the adjustment database, and if so, obtain the same preset score as the animal Score the sleep rhythm regulation scheme associated with the same preset score, and control the sleep rhythm regulation test system to work according to the sleep rhythm regulation scheme. The animal sleep rhythm regulation test method of the present application selects a suitable sleep rhythm regulation scheme according to the basic physiological information of the animal, which can match the animal's own physical condition to the greatest extent.

Description

A kind of animal sleep rhythm control test method

technical field

The application belongs to the field of animal experiment auxiliary technology, and in particular relates to an animal sleep rhythm control test method.

Background technique

In the study of animal nerve function and mechanism, it is sometimes necessary to artificially regulate the sleep of animals, for example, to wake up animals when they go to sleep, or to wake animals up at regular intervals, and to monitor animal sleep under different environmental conditions.

In the prior art, the user usually needs human control or equipment interference to wake up the animal. This method is time-consuming and laborious, and there is a big discrepancy between the modeling itself and the actual situation of sleep rhythm disorder. Moreover, the user usually does not consider the actual situation of the animal, such as the animal's heart condition, blood oxygen condition and other conditions. In this way, on the one hand, it is impossible to perform reasonable sleep regulation according to the actual situation of the animal, and on the other hand, it is easy to damage the For animals, for example, when the animal has a heart problem, if the sleep rhythm regulation test is also performed, the animal may be harmed.

Therefore, it is desirable to have a technical solution to overcome or at least alleviate at least one of the above-mentioned drawbacks of the prior art.

Contents of the invention

The purpose of the present application is to provide an animal sleep rhythm regulation test method to solve at least one of the above-mentioned problems.

In the first aspect of the present application, an animal sleep rhythm regulation test method is used to control the sleep rhythm regulation test system to provide the animal with a sleep rhythm regulation control environment, and the animal is located in the sleep rhythm regulation test device; the animal sleep rhythm Regulatory testing methods include:

Obtain basic physiological information of animals;

Get scoring model;

inputting the basic physiological information of the animal into the scoring model to obtain the animal score;

Acquire an adjustment database, the adjustment database includes at least one sleep rhythm adjustment scheme and at least one preset score, and one sleep rhythm adjustment scheme is associated with at least one preset score;

judging whether the acquired animal score is the same as one of the preset scores in the adjustment database, and if so, then

A sleep rhythm regulation scheme associated with the same preset score as the animal score is obtained, and the sleep rhythm regulation test system is controlled according to the sleep rhythm regulation scheme.

Optionally, the basic physiological information of the animal includes animal body temperature information, animal oxygen content information, animal heart rate information, animal sleep duration information, and animal excitement information.

Optionally, the sleep rhythm regulation scheme includes at least one or more of the following schemes:

The first plan: within 48 hours, light intensity stimulation of 40w was carried out every 30 minutes, and each time lasted 30 minutes; accompanied by noise of 80 to 90 decibels, every 1 hour, each time was 5 minutes;

The second plan: within 36 hours, light intensity stimulation of 60w was carried out every 20 minutes, each time lasted 30 minutes, accompanied by noise of 90 to 110 decibels every 1 hour, each time 2 minutes;

The third scheme: within 72 hours, light intensity stimulation of 50w was carried out every 10 minutes, each time lasted 50 minutes, accompanied by noise of 50 to 70 decibels every 1 hour, each time 10 minutes.

Optionally, during the working process of controlling the sleep rhythm regulation test device according to the sleep rhythm regulation scheme, the animal sleep rhythm regulation test method further includes:

Obtain animal image information;

Identify the image information of the animal to determine whether the animal is sleeping; if so, then

An electrical stimulation signal is generated and delivered to the electrical stimulation system.

Optionally, the identifying the image information of the animal and judging whether the animal falls asleep includes:

Obtain animal image information multiple times at preset time intervals;

Identify whether the animals in the animal image information obtained each time have their eyes closed, and if so, then

Determine if the animal is going to sleep.

Optionally, during the working process of controlling the sleep rhythm regulation test device according to the sleep rhythm regulation scheme, the animal sleep rhythm regulation test method further includes:

Obtain the oxygen content in the sleep rhythm regulation test device;

When the oxygen content is lower than the preset threshold, an oxygen supply signal is generated and transmitted to the sleep rhythm regulation test system to increase the oxygen content in the sleep rhythm regulation test device.

The present application also provides an animal sleep rhythm regulation test device, the animal sleep rhythm regulation test device comprising:

A physiological information acquisition module, the physiological information acquisition module is used to acquire basic physiological information of animals;

A scoring model acquisition module, the scoring model acquisition module is used to acquire a scoring model;

A scoring module, the scoring module is used to input the basic physiological information of the animal into the scoring model, so as to obtain the animal scoring;

An adjustment database acquisition module, the adjustment database acquisition module is used to acquire an adjustment database, the adjustment database includes at least one sleep rhythm adjustment scheme and at least one preset score, and a sleep rhythm adjustment scheme is associated with at least one preset score;

A judging module, the judging module is used to judge whether the obtained animal score is the same as one of the preset scores in the adjustment database;

An execution module, configured to obtain the sleep rhythm adjustment scheme if the judgment module determines that it exists, and control the sleep rhythm adjustment test device to work according to the sleep rhythm adjustment scheme.

The application also provides a sleep rhythm control test system, the sleep rhythm control test system comprising:

A closed box body, the closed box body is provided with an accommodating space;

A conductive plate, the conductive plate is arranged in the accommodating space, and the conductive plate is suitable for placing experimental animals;

A power supply system, the power supply system is connected to the conductive plate, and is used to provide stimulating current for the conductive plate;

An animal basic information detection device, the animal basic information detection device is used to detect the basic physiological information of the animal;

Animal sleep rhythm control test device, the animal sleep rhythm control test device is the above-mentioned animal sleep rhythm control test device, the animal sleep rhythm control test device is respectively connected with the power supply system, oxygen supply device, sound source, oxygen The detector, temperature and humidity detection device, lighting device and camera device are connected to provide working signals for each device connected to it;

an oxygen supply tube, one end of which extends into the containing space;

An oxygen supply device, the oxygen supply device is connected to the other end of the oxygen supply pipe, and the oxygen supply device is used to provide oxygen to the accommodation space through the oxygen supply pipe.

a loudspeaker, the loudspeaker is arranged in the accommodating space;

a sound source connected to the loudspeaker;

an oxygen detector, the oxygen detector is set in the accommodation space, and is used to detect the oxygen content in the accommodation space;

A temperature and humidity detection device, the temperature and humidity detection device is arranged in the accommodation space, and is used to detect the temperature and humidity in the accommodation space;

an illuminating device, the illuminating device is arranged in the accommodating space;

An imaging device, the number of the imaging devices is multiple, and each imaging device is distributed in the accommodating space.

Optionally, the basic animal information detection device includes:

A heart rate monitoring device, the heart rate monitoring device is used to detect animal heart rate information of animals;

A blood oxygen saturation monitor is used for detecting animal oxygen content information of an animal.

The advantages of the animal sleep rhythm regulation test method of the present application are: 1) It can conveniently and effectively construct sleep rhythm disorder models of different strains of animals, saving experimental researchers a lot of energy and labor; The sleep rhythm adjustment scheme can match the animal's own physical condition to the greatest extent, prevent the animal from being harmed, and make the test model more accurate; 3) According to the needs of the research, you can choose to adopt multiple or one way to construct the sleep rhythm disorder The model, flexible formulation and adjustment scheme, can more comprehensively meet the needs of various aspects of the experiment; 4) Using a variety of factors and patterns that affect sleep in daily life to regulate the sleep process is more in line with the existing regulation methods In the actual situation, the interference of other factors is avoided, and the modeling and research results are more convincing.

Description of drawings

Fig. 1 is a schematic flow chart of an animal sleep rhythm regulation test method provided by an embodiment of the present application;

2 is a schematic structural diagram of a sleep rhythm regulation test device provided by an embodiment of the present application;

Fig. 3 is another structural schematic diagram of the sleep rhythm regulation test device provided by one embodiment of the present application;

Fig. 4 is another structural schematic diagram of the sleep rhythm regulation test device provided by one embodiment of the present application;

Fig. 5 is an exemplary structural diagram of an electronic device capable of implementing the test method for regulation and control of animal sleep rhythm according to an embodiment of the present application.

Reference signs:

1. Closed box body; 2. Conductive plate; 3. Oxygen supply tube; 4. Oxygen supply device; 5. Loudspeaker; 6. Sound source; 7. Base plate; 8. Glass cover; 9. Vacuumizing device; 10 1. Oxygen detector; 11. Temperature and humidity detection device; 12. Lighting device; 13. Camera device; 14. Heating device.

Detailed ways

In order to make the purpose, technical solution and advantages of the application more clear, the technical solution in the embodiment of the application will be described in more detail below in conjunction with the drawings in the embodiment of the application. In the drawings, the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The described embodiments are some, but not all, embodiments of the application. The embodiments described below by referring to the figures are exemplary, and are intended to explain the present application, and should not be construed as limiting the present application. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of this application. Embodiments of the present application will be described in detail below in conjunction with the accompanying drawings.

It should be noted that, in the description of the present invention, the terms "first" and "second" are only used for description purposes, and cannot be understood as indicating or implying relative importance.

Fig. 1 is a schematic flowchart of an animal sleep rhythm regulation test method provided by an embodiment of the present application.

The animal sleep rhythm regulation test method shown in FIG. 1 is used to control the sleep rhythm regulation test system to provide animals with a sleep rhythm regulation control environment, and the animals are located in the sleep rhythm regulation test device.

In this embodiment, the animal sleep rhythm regulation test method includes:

Step 1: Obtain basic physiological information of animals;

Step 2: Obtain the scoring model;

Step 3: Input the basic physiological information of the animal into the scoring model to obtain the animal score;

Step 4: Obtain an adjustment database, the adjustment database includes at least one sleep rhythm adjustment scheme and at least one preset score, and one sleep rhythm adjustment scheme is associated with at least one preset score;

Step 5: Determine whether the obtained animal score is the same as the preset score in the adjustment database, and if so, then

Step 6: Obtain a sleep rhythm regulation plan associated with the same preset score as the animal score, and control the sleep rhythm regulation test system to work according to the sleep rhythm regulation plan.

The animal sleep rhythm regulation test method of the present application selects a suitable sleep rhythm regulation scheme based on the basic physiological information of the animal, which can match the animal's own physical condition to the greatest extent, prevent the animal from being harmed, and make the test more accurate.

In this embodiment, the animal's basic physiological information includes animal body temperature information, animal oxygen content information, animal heart rate information, animal sleep duration information, and animal excitement information.

In this example, the scoring model is:

The effect of sleep rhythm regulation is carried out by scoring and accumulating. The regulation indicators include 6 parts: animal body temperature information (T), animal blood oxygen saturation information (SaO ₂ ), animal heart rate information (H), and animal non-sleeping time Information (W), animal movement path distance information (D), animal chest and abdomen movement (B). The formula for calculating the total score is: Σ _Score = (W/36) + [log _1.3 T ₀ - log _1.3 (T _average )] * γ ₁ + [log _0.65 (S _average ) - log _0.65 S ₀ ] * γ ₂ + [log _4.5 H ₀ - log _4.5 (H _average )] * γ ₃ + (log ₂₀₀₀ D ₀ - log ₂₀₀₀ D) * γ ₄ + [lgB ₀ - lg (B _average )] * γ _5. Total score 6 The sum of the parts, each part is scored as -1 to 2 points, and the total score of each part is -5 to 12 points. The higher the total score, the more significant the regulation effect of animal sleep rhythm. Before the test, the value of the scoring coefficient (γ _1-5 ) should be determined according to different types of test animals (such as different breeds of rats and mice, etc.) (Table 1). According to the experimental plan and animal species, each The index has a reference value (T ₀ , S ₀ , H ₀ , W ₀ , D ₀ and B ₀ , Table 2), and each index is measured multiple times and averaged (for example, body temperature can be measured three times, namely T1, T2, T3, add and divide by 3 to obtain T _average ). In this embodiment, the reference value of animal body temperature information is T ₀ , the reference value of animal blood oxygen saturation information is S ₀ , the reference value of animal heart rate information is H ₀ , the reference value of animal non-sleeping time information is W ₀ , and the animal movement path The reference value of the distance information is D ₀ , the reference value of the animal's chest and abdomen movement is B ₀ ; the _average T is the average temperature, the _average S is the average blood oxygen saturation information of the animal, the average H is the average heart rate information of the animal, and _{the average} B is The average value of the animal's chest and abdomen movement, D is the distance of the animal's movement path, and W is the information of the animal's non-sleeping time; the total score (Σ _Score ) is defined as a normal unregulated animal when it is 0 to 2 points; 3 to 5 points are defined as regulated animals ; Greater than or equal to (≥) 6 points were defined as animals with successful regulation of sleep rhythm.

Table 1. Values of scoring coefficients (γ _1-5 ) for different strains of animals

Table 2. Index reference values of different strains of animals

For example, when the animal's body temperature is normal, the score is 0-1; when the animal's body temperature is low, the score is 1-2; when the animal's body temperature is high, the score is -1-0. For example, normal rat animal body temperature is 38.5°C (normal range 37.8 - 39.0°C), 0 to 2 points for body temperature below 38.5°C, and -1 to 0 when the animal is in a high temperature or febrile state, for example, over 38.5°C point.

For example, an animal is considered normoxic and scored 0 to 1 when its oxygen saturation is between 99% and 100%, and is awarded a score of 0-1 when it is below 98%, eg, 80% to 98%. 1 to 2 points, and -1 to 0 points if it is higher than 99.5%.

For example, when the heart rate information of the animal is in a normal state, the score is 0-1, if the heart rate is slow, it is 1-2 points, if the heart rate is fast, it is -1-0 points, for example, the heart rate of a normal rat is 360 For beats/min, if the heart rate is within the normal range, 0 to 1 points will be scored, and if the heart rate is below 260, 1 to 2 points will be scored, and if it is higher than 450, -1 to 0 points will be scored.

For example, when the animal's movement path and distance information are the same as those in the normal state, the score is 0-1. In the case of a day, it belongs to the normal state, with 0 to 1 points, and if it is lower than 8000 meters, it is 1 to 2 points, and if it is greater than 10000 meters per day, it is -1 to 0 points.

For example, when the sleep deprivation duration of animals designed in the experimental scheme is 36 h, 1 point is awarded; when the sleep deprivation duration of animals designed in the experimental scheme is 48 h, 1.5 points are scored; when the sleep deprivation duration of animals designed in the experimental scheme is more than 72 h , then score 2 points.

For example, if the abdominal movement information of the animal is the same as that in the conventional state, it will be scored as 0-1 points, if it is lower than the conventional value, it will be scored as 1-2 points. For example, if the abdominal movement frequency of the rat is 130 times per minute, Normal, 0 to 1 point, if lower than 100 beats per minute, 1 to 2 points, if higher than 150 beats per minute, -1 to 0 points.

Through the acquired data, use the formula to calculate the score, and the final score can be obtained. A total score of 0 to 2 is defined as a normal unregulated animal; a total score of 3 to 5 is defined as a regulated animal; greater than or equal to 6 points is defined as Sleep rhythm regulation in successful animals.

In this embodiment, the sleep rhythm adjustment scheme includes at least one or more of the following schemes:

The first plan: within 48 hours, light intensity stimulation of 40w is carried out every 30 minutes, each time lasts 30 minutes, and the noise is 80 to 90 decibels once every hour, each time is 5 minutes;

The second scheme: within 36 hours, light intensity stimulation of 60w was carried out every 20 minutes, and each time lasted for 30 minutes, and the noise was 90 to 110 decibels once every hour, and each time was 2 minutes;

The third plan: within 72 hours, light intensity stimulation of 50w was carried out every 10 minutes, and each time lasted 50 minutes; noise of 50 to 70 decibels was once every 1 hour, and each time was 10 minutes.

It can be understood that users can also set other sleep rhythm adjustment schemes according to their own needs. It can be understood that a sleep rhythm adjustment scheme can be associated with one preset score or multiple preset scores. For example, in In one embodiment, the preset score is 0 to 100 points, wherein the first scheme is associated with 0 to 40 points, when the animal score is 30 points, the first scheme is used, and the second scheme is associated with 41 to 70 points, The second protocol was used when the animal scored 56 points, the third protocol was associated with 71 to 100 points, and the third protocol was used when the animal scored 80 points.

In this embodiment, during the working process of controlling the sleep rhythm regulation test device according to the sleep rhythm regulation scheme, the animal sleep rhythm regulation test method further includes:

Obtain animal image information;

Identify the image information of the animal to determine whether the animal is sleeping; if so, then

An electrical stimulation signal is generated and delivered to the electrical stimulation system.

In the above-mentioned sleep rhythm adjustment scheme, the animal may perform activities according to the wishes of the user. For example, the animal cannot sleep due to the above-mentioned various disturbances. However, it is also possible that the above-mentioned various disturbances cannot affect the sleep of the animal. At this time, if it is judged that the animal has fallen asleep, an electrical stimulation signal is generated and transmitted to the electrical stimulation system, and the power supply system in the electrical stimulation system supplies power to the conductive plate, thereby stimulating the animal on the conductive plate, thereby causing the animal to wake up.

In this embodiment, identifying the image information of the animal and judging whether the animal is asleep includes:

Obtain animal image information multiple times at preset time intervals;

Identify whether the animals in the animal image information obtained each time have their eyes closed, and if so, then

Determine if the animal is going to sleep.

For example, within 5 minutes, the image information of the animal is obtained every 30 seconds. If it is recognized that the animal is closing its eyes through the image information (whether the animal is closing its eyes can be obtained through image recognition, or the image can be manually observed). method), if so, it is judged that the animal is in sleep.

In this embodiment, during the working process of controlling the sleep rhythm regulation test device according to the sleep rhythm regulation scheme, the animal sleep rhythm regulation test method further includes:

Obtain the oxygen content in the sleep rhythm regulation test device;

When the oxygen content is lower than the preset threshold, an oxygen supply signal is generated and transmitted to the sleep rhythm regulation test system to increase the oxygen content in the sleep rhythm regulation test device.

The present application also provides an animal sleep rhythm control test device, which includes a physiological information acquisition module, a scoring model acquisition module, a scoring module, an adjustment database acquisition module, a judgment module, and an execution module; wherein,

The physiological information acquisition module is used to obtain basic physiological information of animals;

The scoring model obtaining module is used to obtain the scoring model;

The scoring module is used to input the basic physiological information of the animal into the scoring model, so as to obtain the animal scoring;

The adjustment database acquisition module is used to acquire the adjustment database, the adjustment database includes at least one sleep rhythm adjustment scheme and at least one preset score, and one sleep rhythm adjustment scheme is associated with at least one preset score;

The judging module is used to judge whether the obtained animal score is the same as one of the preset scores in the adjustment database;

The execution module is used to acquire the sleep rhythm regulation plan when the judging module judges that there is one, and control the sleep rhythm regulation test device to work according to the sleep rhythm regulation plan.

The present application also provides an electronic device, including a memory, a processor, and a computer program stored in the memory and capable of running on the processor. When the processor executes the computer program, the above-mentioned Animal sleep rhythm regulation test method.

The present application also provides a computer-readable storage medium, the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, it can realize the above-mentioned experimental method for regulating and controlling the sleep rhythm of animals.

Referring to Fig. 2 to Fig. 4, the present application also provides a sleep rhythm control test system, the sleep rhythm control test system includes a closed box body 1, a conductive plate 2, a power supply system, an animal basic information detection device, and an animal sleep rhythm control test system. Test device, oxygen supply pipe 3, oxygen supply device 4, loudspeaker 5, sound source 6, oxygen detector 10, temperature and humidity detection device 11, lighting device 12, camera device 13. in,

An accommodating space is arranged in the closed box body;

A conductive plate is arranged in the accommodating space, and the conductive plate is suitable for placing experimental animals;

A power supply system is connected to the conductive plate for providing stimulating current to the conductive plate;

The animal basic information detection device is used to detect the basic physiological information of the animal;

The animal sleep rhythm regulation and control test device is the above-mentioned animal sleep rhythm control test device, and the animal sleep rhythm control test device is respectively connected with a power supply system, an oxygen supply device, a sound source, an oxygen detector, a temperature and humidity detection device, a lighting device and a camera device. connection, used to provide working signals for each device connected to it;

One end of the oxygen supply pipe extends into the containing space;

The oxygen supply device is connected to the other end of the oxygen supply pipe, and the oxygen supply device is used for providing oxygen to the accommodation space through the oxygen supply pipe.

The loudspeaker is arranged in the containing space;

a sound source is connected to the loudspeaker;

The oxygen detector is set in the accommodation space and is used to detect the oxygen content in the accommodation space;

The temperature and humidity detection device is arranged in the accommodation space, and is used to detect the temperature and humidity in the accommodation space;

The lighting device is arranged in the accommodation space;

There are multiple camera devices, and each camera device is distributed in the accommodation space.

In this embodiment, the animal basic information detection device includes:

A heart rate monitoring device, the heart rate monitoring device is used to detect animal heart rate information of animals; in this embodiment, the heart rate monitoring device adopts the method of subcutaneously implanting electrodes, which is a prior art and will not be repeated here.

A blood oxygen saturation monitor, which is used to detect animal oxygen content information of animals;

Thermometers, thermometers are used to detect the body temperature of animals.

In this embodiment, the animal's basic physiological information includes animal body temperature information, animal oxygen content information, animal heart rate information, animal sleep duration information, animal excitement information, wherein,

The temperature information of the animal is obtained through a thermometer, and it can be understood that the thermometer can be a non-contact thermometer or a contact thermometer.

In this embodiment, the oxygen content information of the animal is obtained through a blood oxygen saturation monitor;

In this embodiment, the heart rate information of the animal is obtained through a heart rate monitoring device;

In this embodiment, the animal's non-sleep duration information is acquired by a camera;

In this embodiment, the animal excitement information is obtained through the camera device. Specifically, the camera device monitors the animal excitement information in real time. The camera device has a timing function. When the controller in the camera device recognizes the If the animal is moving, it is considered that the animal is not sleeping, or the above-mentioned eye-closed information can be used to judge whether the animal is not sleeping, and the moving speed of the animal can also be identified through the image information, for example, whether it is running or walking or staying still.

The sleep rhythm control test system shown in Figures 2 to 4 comprises a closed box body 1, a conductive plate 2, and a power supply system, wherein the closed box body 1 is provided with an accommodating space; the conductive plate 2 is arranged in the accommodating space, The conductive plate is suitable for placing experimental animals; the power supply system is connected with the conductive plate, and is used for providing stimulating current to the conductive plate.

The power supply system in the sleep rhythm regulation test device of the present application provides stimulating current for the conductive plate (the current that will not harm the animal and can provide the animal with a certain stimulation), so as to wake up the animal, thereby controlling the sleep rhythm of the animal, and observing the animal response, and then study the nervous system of animals.

Referring to Fig. 2 to Fig. 4, in the present embodiment, the sleep rhythm control test device further includes an oxygen supply tube 3 and an oxygen supply device 4, and one end of the oxygen supply tube 3 extends into the accommodation space; the oxygen supply device 4 and the oxygen supply tube 3 The other end of the oxygen supply device 4 is used to provide oxygen for the accommodation space through the oxygen supply pipe 3 .

In this way, the oxygen concentration can be adjusted through the oxygen supply device, and the oxygen amount in the accommodation space of the entire closed box body can be increased or decreased according to the test requirements.

In this embodiment, the accommodating space in the closed box body is isolated from the external space, that is, it is in a closed state.

Referring to FIG. 2 to FIG. 4 , in this embodiment, the sleep rhythm control test device further includes a loudspeaker 5 and a sound source 6 , the loudspeaker 5 is arranged in the accommodation space; the sound source 6 is connected to the loudspeaker.

In this way, the animal can be awakened by sound, so as to regulate the sleep of the animal. In addition, this sound can be some high-frequency sound or low-frequency sound, so as to understand the experiment of animals to various sounds during sleep .

Referring to Fig. 3, in this embodiment, the closed box body includes a base plate 7 and a glass cover 8, the glass cover 8 is placed on the base plate 7, and the glass cover 8 and the base plate 7 are enclosed to form an accommodation space; the conductive plate 2 is arranged on the base plate 7 .

Through this arrangement, the user can see the situation in the accommodation space inside the glass cover.

Referring to Fig. 2 to Fig. 4, in this embodiment, the closed box body further includes a base, and the bottom plate is installed on the base.

In this embodiment, the oxygen supply device is installed on the base.

In this embodiment, the base includes a base body and a base baffle set, and the base body is hollow inside for placing various circuits.

In this embodiment, the base baffle set is arranged on the base, and the oxygen supply device and the sound source are installed on the base baffle set.

Referring to FIG. 4 , in this embodiment, the sleep rhythm control test device further includes a vacuum device 9 installed on the bottom plate 7 for vacuuming the accommodation space.

By setting a vacuum device, the air pressure in the holding space can be adjusted, so as to understand the sleeping conditions of animals under different pressure states.

In this embodiment, the sleep rhythm control test device further includes an oxygen detector 10, which is set in the accommodation space and used to detect the oxygen content in the accommodation space.

By setting an oxygen detector, the oxygen content in the accommodation space can be known in real time.

Referring to FIG. 4 , in this embodiment, the sleep rhythm control test device further includes a temperature and humidity detection device 11 , which is set in the storage space and used to detect the temperature and humidity in the storage space.

The temperature and humidity in the accommodation space can be monitored in real time by setting the temperature and humidity detection device.

Referring to FIG. 2 , in this embodiment, the sleep rhythm control test device further includes an illuminating device 12 , and the illuminating device 12 is arranged in the accommodation space.

By setting up the lighting device, it is possible to observe the sleeping state of animals under different light intensities.

Referring to FIG. 2 , in this embodiment, the sleep rhythm control test device further includes a camera device 13 , the number of the camera devices 13 is multiple, and each camera device is distributed in the accommodation space.

Through the camera device, the user can also observe the situation in the accommodating space from a long distance.

Referring to FIG. 2 , in this embodiment, the sleep rhythm control test device further includes a heating device 14 , which is arranged in the glass cover and used for heating the accommodation space in the glass cover.

The temperature and humidity in the accommodation space can be changed by the heating device.

The application also includes a general controller, which is connected with the power supply system, oxygen supply device, sound source 6, vacuum device 9, oxygen detector 10, temperature and humidity detection device 11, lighting device 12, camera device 13 and heating device respectively. 14 connections.

In actual use, the general controller can receive the information transmitted by the above-mentioned various devices connected to it, and control the above-mentioned various devices according to its own control logic.

For example, the master controller obtains the oxygen content detected by the oxygen detector, and sends an operating signal to the oxygen supply device when the oxygen content is lower than the preset oxygen threshold in the master controller, so as to make the oxygen supply device work.

For another example, the general controller acquires temperature information and/or humidity information provided by the temperature and humidity detection device, and provides an operating signal for the heating device when the temperature is lower than the preset temperature in the general controller, thereby heating the heating device.

In this way, on the one hand, various environmental factors can be adjusted according to one's own situation, and on the other hand, various devices can be turned on only when needed, preventing unnecessary waste of various devices and saving resources.

FIG. 5 is an exemplary structural diagram of an electronic device capable of implementing a load balancing method based on a graph convolutional neural network and deep reinforcement learning according to an embodiment of the present application.

As shown in FIG. 5 , the electronic device includes an input device 501 , an input interface 502 , a central processing unit 503 , a memory 504 , an output interface 505 and an output device 506 . Wherein, the input interface 502, the central processing unit 503, the memory 504 and the output interface 505 are connected to each other through the bus 507, and the input device 501 and the output device 506 are respectively connected to the bus 507 through the input interface 502 and the output interface 505, and then connected to other components of the electronic device. Component connections. Specifically, the input device 501 receives input information from the outside, and transmits the input information to the central processing unit 503 through the input interface 502; the central processing unit 503 processes the input information based on computer-executable instructions stored in the memory 504 to generate output information, temporarily or permanently store the output information in the memory 504, and then transmit the output information to the output device 506 through the output interface 505; the output device 506 outputs the output information to the outside of the electronic device for the user to use.

That is to say, the electronic device shown in FIG. 5 can also be implemented as including: a memory storing computer-executable instructions; and one or more processors, which can Realize the experimental method for regulation and control of animal sleep rhythm described in conjunction with FIG. 1 .

In one embodiment, the electronic device shown in FIG. 5 may be implemented to include: a memory 504 configured to store executable program code; one or more processors 503 configured to run the executable code stored in the memory 504. The program code is used to implement the experimental method for regulating the sleep rhythm of animals in the above-mentioned embodiments.

In a typical configuration, a computing device includes one or more processors (CPUs), input/output interfaces, network interfaces, and memory.

Memory may include non-permanent storage in computer-readable media, in the form of random access memory (RAM) and/or nonvolatile memory such as read-only memory (ROM) or flash RAM. Memory is an example of computer readable media.

Computer-readable media include both permanent and non-permanent, removable and non-removable media, which may be implemented by any method or technology for storage of information. Information may be computer readable instructions, data structures, modules of a program, or other data. Examples of computer storage media include, but are not limited to, phase change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), Electrically Erasable Programmable Read Only Memory (EEPROM), Flash memory or other memory technology, Compact Disc Read Only Memory (CD-ROM), Data Versatile Disc (DVD) or other optical storage, A magnetic tape cartridge, tape disk storage or other magnetic storage device, or any other non-transmission medium, that may be used to store information that can be accessed by a computing device.

Those skilled in the art should understand that the embodiments of the present application may be provided as methods, systems or computer program products. Accordingly, the present application can take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present application may take the form of a computer program product embodied on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) having computer-usable program code embodied therein.

Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps. A plurality of units, modules or means stated in the device claims can also be realized by software or hardware by one unit or overall device. The terms first, second, etc. are used to identify names without identifying any particular order.

The flowchart and block diagrams in the Figures illustrate the architecture, functionality, and operation of possible implementations of systems, methods and computer program products according to various embodiments of the present application. In this regard, each block in the flowchart or block diagram may represent a module, program segment, or part of code that includes one or more Executable instructions. It should also be noted that, in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks identified in succession may, in fact, be executed substantially concurrently, or they may sometimes be executed in the reverse order, depending upon the functionality involved. It should also be noted that each block in the block diagrams and/or flowchart illustrations, and combinations of blocks in the block diagrams and/or overall flowchart illustrations, can be implemented by a dedicated hardware-based system that performs the specified functions or operations. implemented, or may be implemented by a combination of special purpose hardware and computer instructions.

The processor referred to in this embodiment may be a central processing unit (Central Processing Unit, CPU), and may also be other general-purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuits) , ASIC), off-the-shelf programmable gate array (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor, or the processor may be any conventional processor, or the like.

The memory can be used to store computer programs and/or modules, and the processor realizes various functions of the device/terminal device by running or executing the computer programs and/or modules stored in the memory and calling the data stored in the memory. The memory can mainly include a program storage area and a data storage area, wherein the program storage area can store an operating system, at least one application program required by a function (such as a sound playback function, an image playback function, etc.); The data created by the use (such as audio data, phone book, etc.) and so on. In addition, the memory can include high-speed random access memory, and can also include non-volatile memory, such as hard disk, internal memory, plug-in hard disk, smart memory card (Smart Media Card, SMC), secure digital (Secure Digital, SD) card , a flash memory card (Flash Card), at least one magnetic disk storage device, flash memory device, or other volatile solid-state storage devices.

In this embodiment, if the integrated module/unit of the device/terminal device is implemented in the form of a software function unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the present invention realizes all or part of the processes in the methods of the above embodiments, and can also be completed by hardware related to computer program instructions. The computer program can be stored in a computer-readable storage medium, and the computer program can be processed. When executed by the controller, the steps in the above-mentioned various method embodiments can be realized. Wherein, the computer program includes computer program code, and the computer program code may be in the form of source code, object code, executable file or some intermediate form. Computer-readable media may include: any entity or device capable of carrying computer program code, recording media, U disk, removable hard disk, magnetic disk, optical disk, computer memory, read-only memory (ROM, Read-Only Memory), random access Memory (RAM, Random Access Memory), electrical carrier signal, telecommunication signal and software distribution medium, etc.

It should be noted that the content contained in the computer readable medium can be appropriately increased or decreased according to the requirements of legislation and patent practice in the jurisdiction. Although the present application is disclosed as above with preferred embodiments, it is not used to limit the present application. Any person skilled in the art can make possible changes and modifications without departing from the spirit and scope of the present application. Therefore, the present application The protection scope of the application shall be defined by the claims of this application.

Finally, it should be pointed out that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: they can still modify the technical solutions described in the aforementioned embodiments, or perform equivalent replacements for some of the technical features; and these The modification or replacement does not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims (7)

1. An animal sleep rhythm control test method is used for controlling a sleep rhythm control test system to provide a sleep rhythm control environment for animals, and the animals are positioned in a sleep rhythm control test device; the animal sleep rhythm regulation test method is characterized by comprising the following steps:

acquiring basic physiological information of animals;

obtaining a grading model;

inputting the basic physiological information of the animal into the scoring model, thereby obtaining animal scoring;

acquiring a regulation database, wherein the regulation database comprises at least one sleep rhythm regulation scheme and at least one preset score, and the sleep rhythm regulation scheme is at least associated with the preset score;

judging whether the obtained animal score is the same as one preset score in all preset scores in the regulation database, if so, judging whether the obtained animal score is the same as one preset score in all preset scores in the regulation database, and if so, judging whether the obtained animal score is the same as one preset score in all preset scores in the regulation database

Acquiring a sleep rhythm regulation scheme associated with a preset score which is the same as the animal score, and controlling a sleep rhythm regulation test system to work according to the sleep rhythm regulation scheme; wherein,

the basic physiological information of the animal comprises animal body temperature information, animal blood oxygen saturation information, animal heart rate information, animal sleep time length information, animal movement path and distance information and animal chest and abdomen movement information;

the sleep rhythm regulation scheme includes at least one or more of the following:

the first scheme is as follows: within 48 hours, the stimulation is carried out every 30 minutes by illumination with 40w intensity, each time lasts for 30 minutes, and the stimulation is carried out every 1 hour by noise of 80-90 dB, each time lasts for 5 minutes;

the second scheme is as follows: within 36 hours, the light 60w intensity stimulation is performed every 20 minutes, each time lasts for 30 minutes, and the noise is 90-110 decibels, and every 1 hour is performed every 2 minutes;

the third scheme is as follows: within 72 hours, the stimulation with the intensity of 50w of light is carried out every 10 minutes, each time lasts for 50 minutes, and the stimulation with the noise of 50-70 decibels is carried out every 1 hour, each time lasts for 10 minutes;

in the process of controlling the work of the sleep rhythm control test device according to the sleep rhythm control scheme, the animal sleep rhythm control test method further comprises the following steps:

acquiring animal image information;

identifying the animal image information and judging whether the animal goes to sleep or not; if so, then

Generating an electrical stimulation signal and transmitting the electrical stimulation signal to an electrical stimulation system;

the scoring model is as follows:

Σ _Score =（W/36）+〔log _1.3 T ₀ - log _1.3 （T _average ）〕* γ ₁ +〔log _0.65 （S _Average ）- log _0.65 S ₀ 〕* γ ₂ + 〔log _4.5 H ₀ - log _4.5 （H _Average ）〕* γ ₃ +（log ₂₀₀₀ D ₀ - log ₂₀₀₀ D）* γ ₄ +〔lgB ₀ -lg（B _Average ）〕* γ ₅ (ii) a Wherein,

the reference value of the animal body temperature information is T ₀ The reference value of the blood oxygen saturation information of the animal is S ₀ And the animal heart rate information reference value is H ₀ The reference value of the information of the non-sleeping time length of the animal is W ₀ The reference value of the animal movement path distance information is D ₀ The reference value of the chest and abdomen movement condition of the animal is B ₀ ；T _Average Is the mean value of body temperature, S _{Average out} The average value of the animal blood oxygen saturation information is H average value of the animal heart rate information, B _Average The average value of the motion conditions of the chest and the abdomen of the animal, D is the motion path distance of the animal, and W is the information of the duration of the animal not sleeping; wherein,

when the animal is a mouse, γ ₁ Is 4, gamma ₂ Is 4, gamma ₃ Is 10, gamma ₄ Is 8, gamma ₅ Is 10, T ₀ At 38.0 ℃ and S ₀ 99.5% of H ₀ Is 650 times/min, W ₀ Is 36 smallHour and D ₀ 10000 m, B ₀ 175 times/min;

when the animal is a rat, γ ₁ Is 3, gamma ₂ Is 4, gamma ₃ Is 8, gamma ₄ Is 9, gamma ₅ Is 14, T ₀ At 38.5 ℃ and S ₀ 99.5% of H ₀ Is 360 times/min, W ₀ 36 hours, D ₀ 8000 m, B ₀ 130 times/min;

when the animal is guinea pig, γ ₁ Is 5, gamma ₂ Is 4, gamma ₃ Is 5, gamma ₄ Is 10, gamma ₅ Is 16, T ₀ At 38.2 ℃ and S ₀ 99.5% of H ₀ 220 times/min, W ₀ 36 hours, D ₀ Is 5000 m, B ₀ Is 105 times/min.

2. The animal sleep rhythm control test method as set forth in claim 1, wherein the animal sleep rhythm control test method further comprises, during controlling the operation of the sleep rhythm control test device according to the sleep rhythm control program:

acquiring animal image information;

identifying the animal image information and judging whether the animal goes to sleep or not; if so, then

Generating an electrical stimulation signal and transmitting the electrical stimulation signal to an electrical stimulation system.

3. The animal sleep rhythm control test method as set forth in claim 2, wherein the recognizing the animal image information and determining whether the animal is asleep comprises:

acquiring animal image information for multiple times at preset time intervals;

identifying whether the animals in the animal image information obtained at each time are all closed eyes, if yes, then

And judging that the animal enters the sleep.

4. The animal sleep rhythm control test method as set forth in claim 3, wherein the animal sleep rhythm control test method further comprises, during controlling the operation of the sleep rhythm control test device according to the sleep rhythm control program:

acquiring the oxygen content in the sleep rhythm regulation test device;

and when the oxygen content is lower than a preset threshold value, generating an oxygen supply signal and transmitting the oxygen supply signal to the sleep rhythm regulation and control test system to improve the oxygen content in the sleep rhythm regulation and control test device.

5. An animal sleep rhythm control test device, characterized in that, the animal sleep rhythm control test device includes:

the physiological information acquisition module is used for acquiring basic physiological information of the animal;

the scoring model acquisition module is used for acquiring a scoring model;

the scoring module is used for inputting the basic physiological information of the animal into the scoring model so as to obtain animal scoring;

a conditioning database acquisition module for acquiring a conditioning database, the conditioning database comprising at least one sleep rhythm conditioning regimen and at least one preset score, one sleep rhythm conditioning regimen being associated with at least one preset score;

the judgment module is used for judging whether the obtained animal score is the same as one preset score in all preset scores in the regulation database;

the execution module is used for acquiring the sleep rhythm regulation scheme under the condition that the judgment module judges that the sleep rhythm regulation scheme exists, and controlling the sleep rhythm regulation test device to work according to the sleep rhythm regulation scheme; wherein,

the basic physiological information of the animal comprises animal body temperature information, animal blood oxygen saturation information, animal heart rate information, animal sleep-free time length information, animal movement path and distance information and animal chest and abdomen movement information;

the sleep rhythm regulation scheme includes at least one or more of the following:

the first scheme is as follows: within 48 hours, the stimulation is carried out every 30 minutes at the intensity of 40w of illumination, each time lasts for 30 minutes, and the stimulation is carried out every 1 hour at the noise of 80-90 decibels, each time lasts for 5 minutes;

the second scheme is as follows: within 36 hours, the light 60w intensity stimulation is carried out every 20 minutes, each time lasts for 30 minutes, and the noise is 90-110 decibels every 1 hour and each time lasts for 2 minutes;

the third scheme is as follows: within 72 hours, the stimulation with the intensity of 50w of light is carried out every 10 minutes, each time lasts for 50 minutes, and the stimulation with the noise of 50-70 decibels is carried out every 1 hour, each time lasts for 10 minutes;

in the process of controlling the work of the sleep rhythm control test device according to the sleep rhythm control scheme, the animal sleep rhythm control test method further comprises the following steps:

acquiring animal image information;

identifying the animal image information and judging whether the animal goes to sleep or not; if so, then

Generating an electrical stimulation signal and transmitting the electrical stimulation signal to an electrical stimulation system;

the scoring model is as follows:

Σ _Score =（W/36）+〔log _1.3 T ₀ - log _1.3 （T _average ）〕* γ ₁ +〔log _0.65 （S _{Average out} ）- log _0.65 S ₀ 〕* γ ₂ + 〔log _4.5 H ₀ - log _4.5 （H _{Average out} ）〕* γ ₃ +（log ₂₀₀₀ D ₀ - log ₂₀₀₀ D）* γ ₄ +〔lgB ₀ -lg（B _Average ）〕* γ ₅ (ii) a Wherein,

the reference value of the animal body temperature information is T ₀ The reference value of the blood oxygen saturation information of the animal is S ₀ The animal heart rate information reference value is H ₀ The reference value of the information of the length of time when the animal does not sleep is W ₀ The reference value of the animal movement path distance information is D ₀ The reference value of the chest and abdomen movement condition of the animal is B ₀ ；T _Average Is the mean value of body temperature、S _Average The average value of the blood oxygen saturation information of the animal, the average value of the H average information of the heart rate of the animal, B _{Average out} The average value of the chest and abdomen movement conditions of the animal, D the movement path distance of the animal and W the information of the non-sleeping time length of the animal are obtained; wherein,

when the animal is a mouse, γ ₁ Is 4, gamma ₂ Is 4, gamma ₃ Is 10, gamma ₄ Is 8, gamma ₅ Is 10, T ₀ At 38.0 ℃ and S ₀ 99.5% of H ₀ Is 650 times/min, W ₀ 36 hours, D ₀ 10000 m, B ₀ 175 times/min;

when the animal is a rat, γ ₁ Is 3, gamma ₂ Is 4, gamma ₃ Is 8, gamma ₄ Is 9, gamma ₅ Is 14, T ₀ At 38.5 ℃ and S ₀ 99.5% of H ₀ Is 360 times/min, W ₀ 36 hours, D ₀ 8000 m, B ₀ 130 times/min;

when the animal is a guinea pig, γ ₁ Is 5, gamma ₂ Is 4, gamma ₃ Is 5, gamma ₄ Is 10, gamma ₅ Is 16, T ₀ At 38.2 ℃ and S ₀ 99.5% of H ₀ Is 220 times/min, W ₀ 36 hours, D ₀ Is 5000 m, B ₀ Is 105 times/min.

6. A sleep rhythm control test system, characterized in that the sleep rhythm control test system comprises:

the box comprises a closed box body, wherein an accommodating space is arranged in the closed box body;

the conductive plate is arranged in the accommodating space and is suitable for placing a test animal;

the power supply system is connected with the conductive plate and used for providing stimulation current for the conductive plate;

the animal basic information detection device is used for detecting basic physiological information of the animal;

the animal sleep rhythm control test device according to claim 5, wherein the animal sleep rhythm control test device is respectively connected with the power supply system, the oxygen supply device, the sound source, the oxygen detector, the temperature and humidity detection device, the illumination device and the camera device and is used for respectively providing working signals for each device connected with the animal sleep rhythm control test device;

one end of the oxygen supply pipe extends into the accommodating space;

the oxygen supply device is connected with the other end of the oxygen supply pipe and is used for supplying oxygen to the accommodating space through the oxygen supply pipe;

a microphone disposed within the accommodating space;

the sound source is connected with the loudspeaker;

the oxygen detector is arranged in the accommodating space and used for detecting the oxygen content in the accommodating space;

the temperature and humidity detection device is arranged in the accommodating space and is used for detecting the temperature and the humidity in the accommodating space;

an illumination device disposed within the accommodation space;

the number of the camera devices is multiple, and the camera devices are distributed in the accommodating space.

7. The sleep rhythm control test system as set forth in claim 6, wherein the animal basic information detecting means includes:

a heart rate monitoring device for detecting animal heart rate information of an animal;

the system comprises a blood oxygen saturation monitor, a data processing unit and a data processing unit, wherein the blood oxygen saturation monitor is used for detecting animal oxygen content information of animals.

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