CN1139114A - Prepn process of adenosine triphosphate from hypoxanthine nucleotide - Google Patents
Prepn process of adenosine triphosphate from hypoxanthine nucleotide Download PDFInfo
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- CN1139114A CN1139114A CN 95106298 CN95106298A CN1139114A CN 1139114 A CN1139114 A CN 1139114A CN 95106298 CN95106298 CN 95106298 CN 95106298 A CN95106298 A CN 95106298A CN 1139114 A CN1139114 A CN 1139114A
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- reaction
- yeast
- triphosaden
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- glucose
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Abstract
The said prepn process is that in the presence of ammonium ion and magnesium ion, hypoxanthine nucleotide is converted into adenosine monophosphate, which is then converted into adenosine triphosphate via fermentation with yeast. It has low cost and high conversion.
Description
The present invention relates to biochemical field, particularly a kind of preparation method of Triphosaden.
Triphosaden (hereinafter to be referred as ATP) is a kind of Nucleotide medicine, and it contains high-energy phosphate bond, is energy converter, reservoir, plays an important role in human energy metabolism.Using it clinically always and do the first aid medication, also is important adjuvant drug.Develop its enteric coated tablet in recent years, be used as the medicine that cures mainly of gastroptosis.ATP also can do the agent that boils together and use in food.
What the preparation method of ATP adopted the earliest is to extract from rabbit flesh, or synthesizes ATP with the photosynthesis of spinach.Production method commonly used at present is to make raw material with adenylic acid (AMP) and adenosine (AR), produces ATP with yeast fermentation method, and this method exists the high problem of cost.
The purpose of this invention is to provide a kind of method of utilizing inosinic acid (IMP) to make the feedstock production Triphosaden.
Method of the present invention makes adenylic acid change into Triphosaden by yeast fermentation for to make inosinic acid change into adenylic acid in the presence of glucose, yeast, ammonium ion and magnesium ion then.
Ammonium ion in the described method especially preferably uses ammonium chloride or ammonium hydroxide as the donor of amino.Described magnesium ion plays katalysis, available magnesium chloride.Wherein the IMP temperature of reaction that changes into AMP is preferably 28～32 ℃, preferred 10～24 hours of reaction times, reaction system parts by weight formula optimization: IMP0.1～0.2 part, NH
4OH (NH
4Cl) 0.02～0.1 part, MgCl
20.05～0.3 part, 0.6～1.5 part of glucose, 3～4 parts in yeast.
The adenylic acid of the inventive method changes in the reaction system of Triphosaden, particularly contain glucose, yeast, potassium primary phosphate and magnesium chloride, also the available hydrogen sodium oxide is transferred PH, as transfer to PH7, preferred 28～32 ℃ of this temperature of reaction, preferred 6～8 hours of reaction times, the parts by weight proportion optimization of described reaction system is: 0.5～1 part of potassium primary phosphate, 0.05～0.1 part in magnesium chloride, 1～2 part of glucose, 4～6 parts in yeast, the amount of AMP is in 0.1～0.2 part of amount that IMP was changed into.
Key of the present invention is the step that IMP changes into AMP.In the prior art, IMP transforms and generates AMP and GMP (guanylic acid), and the transformation efficiency that IMP changes into AMP has only about 50%.And IMP only generates AMP basically in the method for the invention, does not almost have GMP to generate.Make low-cost, high conversion ground become possibility like this, opened up the new of ATP production, feasible way by IMP production ATP.
So the transformation efficiency that present method makes IMP generate AMP has improved nearly one times, greatly reduces the production cost of ATP.In addition, present method reaction conditions is simple, and speed of response is very fast.
Further specify the present invention below by embodiment.
Get IMP0.15 gram, NH
4OH60 μ M, MgCl
20.15 gram, yeast 3.5 grams, mix the back and reacted 15 hours about 30 ℃, add 0.75 gram potassium primary phosphate, 0.08 gram magnesium chloride, 1.5 gram glucose then after aftertreatment, transferring PH with sodium hydroxide is 7, cumulative volume is 10 milliliters in the aqueous solution, and reaction is 7 hours about 30 ℃.
Reaction finishes the logical steam heating (100 ℃) in back 5～10 minutes, and centrifugal (3000～5000 rev/mins) 5～10 minutes get supernatant liquor, and with 3 times of deionized water dilutions, last ion exchange resin column separates.For obtaining being fit to the ATP of injection medicine inspection standard, after separating with anionite-exchange resin (polystyrene-divinylbenzene quaternary amine type, degree of crosslinking 1 * 8, granularity 100～200 orders) post, also need with the diatomite source of reducing phlegm and internal heat.For obtaining being fit to the ATP of tablet medicine inspection standard, after the anion-exchange resin column separation, use cloudy post (or good activated carbon column) to concentrate again, elute and promptly can be used as compressing tablet.
Post treatment method can adopt any suitable method of prior art, can be with reference to " production of ucleotides material and application " data compilation, Science Press, 1971.
Claims (10)
1. the preparation method of a Triphosaden is characterized in that making in the presence of ammonium ion inosinic acid to change into adenylic acid, makes adenylic acid change into Triphosaden by yeast fermentation then.
2. method as claimed in claim 1 is characterized in that also having glucose, yeast, magnesium ion in the reaction system of inosinic acid.
3. as the method for claim 1 or 2, it is characterized in that described ammonium ion is with ammonium chloride or ammonium hydroxide.
4. method as claimed in claim 2 is characterized in that described magnesium ion magnesium chloride.
5. as the method for claim 1 or 2, it is characterized in that temperature of reaction is 28～32 ℃.
6. method as claimed in claim 2 is characterized in that the reaction system parts by weight prescription of inosinic acid is: IMP0.1～0.2 part, NH
4OH (or NH
4Cl) 0.02～0.1 part, MgCl
20.05～0.3 part, 0.6～1.5 part of glucose, 3～4 parts in yeast, temperature of reaction is 28～32 ℃, 10～24 hours reaction times.
7. as the method for claim 1 or 2, it is characterized in that adenylic acid changes in the reaction system of Triphosaden, contains glucose, yeast, potassium primary phosphate and magnesium chloride.
8. method as claimed in claim 7, it is characterized in that the parts by weight proportioning that adenylic acid changes into the reaction system of Triphosaden is 0.5～1 part of a potassium primary phosphate, 0.05～0.1 part in magnesium chloride, 1～2 part of glucose, 4～6 parts in yeast, the amount of AMP is in 0.1～0.2 part of amount that IMP was changed into, and temperature of reaction is 28～32 ℃, and the reaction times is 6～8 hours.
9. method as claimed in claim 7 is characterized in that the reaction system that AMP changes into ATP regulates pH value with sodium hydroxide.
10. as the method for claim 1 or 2, it is characterized in that transforming obtain ATP after, logical steam heating 5～10 minutes, centrifugal (3000～5000 rev/mins) 5～10 minutes get supernatant liquor, after the deionized water dilution, last ion exchange resin column separates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95106298 CN1139114A (en) | 1995-06-28 | 1995-06-28 | Prepn process of adenosine triphosphate from hypoxanthine nucleotide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95106298 CN1139114A (en) | 1995-06-28 | 1995-06-28 | Prepn process of adenosine triphosphate from hypoxanthine nucleotide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1139114A true CN1139114A (en) | 1997-01-01 |
Family
ID=5075861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95106298 Pending CN1139114A (en) | 1995-06-28 | 1995-06-28 | Prepn process of adenosine triphosphate from hypoxanthine nucleotide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1139114A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109851650A (en) * | 2018-12-19 | 2019-06-07 | 新疆阜丰生物科技有限公司 | A method of extracting adenosine from fermentation liquid |
| WO2019109560A1 (en) * | 2017-12-05 | 2019-06-13 | 中国科学院化学研究所 | Method for extracting adenosine triphosphate from adenosine triphosphate solution |
-
1995
- 1995-06-28 CN CN 95106298 patent/CN1139114A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019109560A1 (en) * | 2017-12-05 | 2019-06-13 | 中国科学院化学研究所 | Method for extracting adenosine triphosphate from adenosine triphosphate solution |
| CN109851650A (en) * | 2018-12-19 | 2019-06-07 | 新疆阜丰生物科技有限公司 | A method of extracting adenosine from fermentation liquid |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |