CN113908901A - Extracting solution liquid-transfering gun of exosome production usefulness - Google Patents

Extracting solution liquid-transfering gun of exosome production usefulness Download PDF

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Publication number
CN113908901A
CN113908901A CN202111411869.1A CN202111411869A CN113908901A CN 113908901 A CN113908901 A CN 113908901A CN 202111411869 A CN202111411869 A CN 202111411869A CN 113908901 A CN113908901 A CN 113908901A
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China
Prior art keywords
gun
transition box
box
fixedly connected
rope
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Granted
Application number
CN202111411869.1A
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Chinese (zh)
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CN113908901B (en
Inventor
徐晓
段莉
徐丽梅
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Shenzhen Second Peoples Hospital
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Shenzhen Second Peoples Hospital
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Priority to CN202111411869.1A priority Critical patent/CN113908901B/en
Publication of CN113908901A publication Critical patent/CN113908901A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0213Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227Details of motor drive means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237Details of electronic control, e.g. relating to user interface

Abstract

The invention discloses an extracting solution pipetting gun for exosome production, which comprises a gun body, wherein a piston rod is movably connected onto the gun body, an adjusting knob is rotatably arranged on the piston rod, a volume display is fixedly arranged on the gun body, a transition box is fixedly connected onto the gun body, a bent pipe is fixedly connected into the transition box, a connector is fixedly connected onto the bent pipe, a gun head pipe is fixedly connected onto the connector, a level sensor is fixedly arranged into the transition box, the level sensor is electrically connected with an alarm, a gun head locking structure with an electromagnetic unlocking function and an electromagnet are arranged in the transition box, and a gun head counterweight structure with an electromagnetic unlocking function is arranged in the transition box; the pipetting gun has the function of self-adjusting the verticality in a small range, and when the pipetting gun is used, an operator only needs to keep the approximate vertical state of the pipetting gun, so that the pipetting operation can be completed in a relatively perfect vertical state, and the difficulty of the pipetting operation of the extracting solution is reduced.

Description

Extracting solution liquid-transfering gun of exosome production usefulness
Technical Field
The invention relates to the technical field of biological medicine manufacturing, in particular to an extracting solution pipetting gun for exosome production.
Background
The manufacture of biological medicines has important significance for the development of medicine, and the manufacture of biological medicines can optimize the existing treatment method and break through some difficult and complicated diseases. The biological medicine material is mainly natural biological material, including microbe, human body, animal, plant, marine organism, etc. With the development of biotechnology, purposely artificially prepared biological raw materials become the main source of the current biopharmaceutical raw materials. Such as animal material prepared by immunization, microorganism or other cell material prepared by changing gene structure, etc. The biological medicine has the features of high pharmacological activity, less toxic side effect and high nutritive value.
The preparation method of the biological medicine is various, and mainly needs extraction and directional culture of biological raw materials, so that the biological raw materials become nontoxic and harmless medicines. The exosome is an important biological medicine, has a certain microscopic treatment effect, more importantly is present as a medicine carrier, and has important significance for the manufacture of the biological medicine.
In the process of exosome production, it is extracted first, and for exosome extract, a liquid-transfering gun is often used for quantitative suction transfer. Present liquid-transfering gun is higher to the requirement of standard operation, and especially liquid-transfering gun inclination when imbibing requires very high, and the liquid is got in the slope not only forms the bubble easily, leads to moving the liquid inaccurate, and liquid gets into pipettor sleeve handle easily simultaneously for piston spring suffers the corruption, and further influence the precision of liquid-transfering gun. Damage to the pipette not only delays the production of exosome drugs, but also directly causes economic loss because the current pipette is sold at thousands yuan. In view of this, we propose an extract pipetting gun for exosome production.
Disclosure of Invention
The invention aims to provide an extracting solution pipetting gun for exosome production, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an extracting solution pipetting gun for exosome production comprises a gun body, wherein a piston rod is movably connected onto the gun body, an adjusting knob is rotatably mounted on the piston rod, a volume display is fixedly mounted on the gun body, a transition box is fixedly connected onto the gun body, a bent pipe is fixedly connected into the transition box, a connector is fixedly connected onto the bent pipe, a gun head pipe is fixedly connected onto the connector, a level sensor is fixedly mounted into the transition box, the level sensor is electrically connected with an alarm, a side plate is fixedly mounted onto the connector, a positioning hole is formed in the side plate, a gun head locking structure with an electromagnetic unlocking function and an electromagnet are mounted into the transition box, a gun head counterweight structure with an electromagnetic unlocking function is mounted into the transition box, a bearing plate is fixedly mounted onto the gun head pipe, and a driving structure with unbalanced starting is mounted into the transition box, and the driving structure is connected with a rope structure with a displacement amplification function, and the rope structure is connected to a locking piece which is installed through a torsion spring shaft and a gun body.
Preferably, the gun head locking structure comprises a mounting box fixedly mounted in the transition box, a first spring is fixedly mounted in the mounting box, a positioning block is fixedly connected to the first spring, a first magnetic block is fixedly mounted on the positioning block, the mounting box is located on the side face of the side plate and is slidably mounted in the mounting box, the end portion of the positioning block penetrates through the side face of the mounting box, and the first magnetic block is fixedly mounted at the top of the positioning block.
Preferably, the gun head counterweight structure and the gun head locking structure are respectively located on two sides of the electromagnet, the gun head counterweight structure comprises an inner sliding seat which is slidably mounted in the transition box, a second spring and a second magnetic block are fixedly connected to the inner sliding seat, a connecting rope is fixedly connected to the inner sliding seat, and a counterweight block is fixedly connected to the connecting rope.
Preferably, second spring fixedly connected with is between transition box inner wall and interior sliding seat, and second magnetic path and first magnetic path adopt the opposite installation of magnetic pole orientation, the electro-magnet is located the middle part of first magnetic path and second magnetic path, the bottom of transition box is provided with the recess, and the balancing weight is located the recess, interior sliding seat and balancing weight are located the box of transition box respectively and outside the box, and connect the rope and connect between the two.
Preferably, the driving structure comprises a back plate fixedly installed in the transition box, a telescopic rod is fixedly installed on the back plate, a movable rod is fixedly connected onto the telescopic rod, a push rod is fixedly installed on the movable rod, and the push rod is slidably installed in the guide rail.
Preferably, the backplate is connected perpendicularly on the inner wall of transition box, and the telescopic link adopts electric telescopic link, and telescopic link and level sensor electric connection, movable rod fixed connection is at the tip of telescopic link, and catch bar and movable rod perpendicular connection, the catch bar is connected with the return bend contact, and guided way fixed mounting is on the inner wall of transition box.
Preferably, the rope structure is including the stay cord with drive structure fixed connection, and stay cord fixed connection is on hard lever, the last fixedly connected with of hard lever unlocks the rope, and unlocks rope swing joint on the steering column, and unlocks the rope through connection in the fag end.
Preferably, hard lever is installed in the transition box through the pivot, and the stay cord is connected with the short arm of force of hard lever, and the unlocking rope is connected with the long arm of force of hard lever, steering column fixed connection is on the inner wall of transition box, and fag end fixed mounting in the body of a gun, the locking piece passes through the torsional spring axle and installs the side at adjust knob, and the end connection of unlocking rope and locking piece.
Preferably, transition box fixed mounting is in the bottom of body of a gun, and the return bend communicates with the air cavity of body of a gun, the bottom at the return bend is connected to the connector, and rifle head pipe and connector joint, level sensor installs the central point at the transition box and puts, and the alarm is audible alarm.
Compared with the prior art, the invention has the beneficial effects that:
1. the liquid-transferring gun is improved in the production process of exosomes in the biological medicine, so that the preparation quality of the biological medicine can be effectively improved, the liquid-transferring gun has the function of self-adjusting the verticality in a small range, and an operator only needs to keep the approximate vertical state of the liquid-transferring gun when the liquid-transferring gun is used, so that the liquid-transferring operation can be completed in a relatively perfect vertical state, and the difficulty of the liquid-transferring operation of an extracting solution in the production of the exosomes is reduced;
2. the transition box is used as a transition connection structure of the gun body and the gun head pipe, the bent pipe is arranged in the transition box and is connected between the gun body and the gun head pipe, the length of the bent pipe can be discharged if necessary, the gun head pipe is connected in a suspension manner, the gun head pipe is automatically suspended and kept in a vertical state under the action of gravity, the operation error can be reduced to a certain extent, an operator only needs to roughly ensure that the pipette is approximately vertical, perfect operation can be carried out, the operation difficulty of the pipette is reduced, the pipette is not easy to damage, a level sensor arranged in the transition box can monitor the levelness, when the verticality of the pipette is within an acceptable range, an alarm stops giving an alarm, and further driving of a gun head locking structure and a gun head counterweight structure is carried out, so that the gun head pipe is suspended, and the self-modification effect is achieved, the use is very convenient;
3. according to the invention, the gun head locking structure is used for fixing the connector, so that the gun head pipe can be fixedly connected to the gun body under normal conditions, and when the horizontal sensor detects that the gun head pipe has a fine adjustment space, the electromagnet can be electrified to unlock the connector, and the connector has activity;
4. the driving structure works when the level sensor detects that self-modification can be carried out, on one hand, extrusion on the bent pipe is released, so that the connector has a movable space, on the other hand, the rope structure is driven, the driving structure drives the rope structure and can act on the locking piece, so that the locking piece rotates through the position of the bottom of the adjusting knob and does not block the pressing of the adjusting knob, the normal liquid taking work of the liquid-transferring gun can be indicated, and the damage to the liquid-transferring gun caused by misoperation is prevented.
Drawings
FIG. 1 is a schematic diagram of the front structure of the present invention;
FIG. 2 is a schematic view of a backside structure of the present invention;
FIG. 3 is a schematic view of the overall structure of the present invention;
FIG. 4 is a schematic illustration of the locked state of the present invention;
FIG. 5 is a schematic view of an internal module of the present invention;
FIG. 6 is an enlarged view of area A in FIG. 5;
FIG. 7 is a schematic view of the structure of a lance head according to the present invention;
fig. 8 is a sectional view of a coupling structure of the lance head according to the present invention;
FIG. 9 is a schematic view of a connection structure of the present invention;
FIG. 10 is a schematic view of the locking mechanism of the present invention;
fig. 11 is an enlarged view of the area B in fig. 9.
In the figure: the gun body 1, the piston rod 2, the adjusting knob 3, the volume display 4, the transition box 5, the elbow 6, the connector 7, the gun head pipe 8, the level sensor 9, the alarm 10, the side plate 11, the positioning hole 12, the mounting box 13, the first spring 14, the positioning block 15, the first magnet 16, the electromagnet 17, the back plate 18, the telescopic rod 19, the movable rod 20, the push rod 21, the guide rail 22, the bearing plate 23, the inner sliding seat 24, the second spring 25, the second magnet 26, the connecting rope 27, the balancing weight 28, the pull rope 29, the labor-consuming lever 30, the unlocking rope 31, the steering rod 32, the rope sleeve 33, the locking piece 34 and the torsion spring shaft 35.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 11, the present invention provides a technical solution: an extracting solution pipetting gun for exosome production comprises a gun body 1, wherein a piston rod 2 is movably connected to the gun body 1, an adjusting knob 3 is rotatably installed on the piston rod 2, a volume display 4 is fixedly installed on the gun body 1, a transition box 5 is fixedly connected to the gun body 1, a bent pipe 6 is fixedly connected to the transition box 5, a connector 7 is fixedly connected to the bent pipe 6, a gun head pipe 8 is fixedly connected to the connector 7, a level sensor 9 is fixedly installed in the transition box 5, the level sensor 9 is electrically connected with an alarm 10, a side plate 11 is fixedly installed on the connector 7, a positioning hole 12 is formed in the side plate 11, a gun head locking structure with an electromagnetic unlocking function and an electromagnet 17 are installed in the transition box 5, a gun head counterweight structure with an electromagnetic unlocking function is installed in the transition box 5, and a bearing plate 23 is fixedly installed on the gun head pipe 8, the transition box 5 is provided with a driving structure with unbalance starting, the driving structure is connected with a rope structure with displacement amplification function, and the rope structure is connected on a locking piece 34 which is arranged with the gun body 1 through a torsion spring shaft 35.
The gun head locking structure comprises a mounting box 13 fixedly mounted in the transition box 5, a first spring 14 is fixedly mounted in the mounting box 13, a positioning block 15 is fixedly connected to the first spring 14, a first magnetic block 16 is fixedly mounted on the positioning block 15, the mounting box 13 is located on the side face of the side plate 11, the positioning block 15 is slidably mounted in the mounting box 13, the end portion of the positioning block 15 penetrates through the side face of the mounting box 13, and the first magnetic block 16 is fixedly mounted at the top of the positioning block 15;
the invention utilizes the gun head locking structure to fix the connector 7, thereby ensuring that the gun head pipe 8 can be fixedly connected on the gun body 1 under the normal condition;
the positioning block 15 is pushed by the elastic force of the first spring 14, so that the positioning block 15 protrudes out of the mounting box 13 and is connected with the positioning hole 12 on the side plate 11, the gun head locking structure is provided with a left gun head locking structure and a right gun head locking structure, the connector 7 can be stably positioned in the transition box 5 and is connected with the gun head pipe 8, when the level sensor 9 detects that the gun head pipe 8 has a fine adjustment space, the electromagnet 17 can be electrified, the first magnetic block 16 is subjected to magnetic attraction, the positioning block 15 retracts, the connector 7 is unlocked, and the connector 7 has activity;
the gun head counterweight structure and the gun head locking structure are respectively positioned at two sides of the electromagnet 17, the gun head counterweight structure comprises an inner sliding seat 24 which is slidably arranged in the transition box 5, a second spring 25 and a second magnetic block 26 are fixedly connected on the inner sliding seat 24, a connecting rope 27 is fixedly connected on the inner sliding seat 24, and a balancing weight 28 is fixedly connected on the connecting rope 27;
considering that the weight of the gun head pipe 8 is light, although the gun head pipe can be suspended due to the activity, the gun head pipe is not easy to keep vertical, and therefore the gun head pipe is further provided with the gun head counterweight structure, pressure cannot be caused on the gun head pipe 8 under normal conditions, and the gun head pipe can be balanced and pressed on the gun head pipe 8 when self-modification is carried out;
the second spring 25 is fixedly connected between the inner wall of the transition box 5 and the inner sliding seat 24, the second magnetic block 26 and the first magnetic block 16 are installed in a mode that the magnetic poles face oppositely, the electromagnet 17 is located in the middle of the first magnetic block 16 and the second magnetic block 26, a groove is formed in the bottom of the transition box 5, the balancing weight 28 is located in the groove, the inner sliding seat 24 and the balancing weight 28 are located inside and outside the transition box 5 respectively, and the connecting rope 27 is connected between the inner sliding seat and the balancing weight;
generally, the elastic force of the second spring 25 acts on the inner sliding seat 24, the inner sliding seat 24 pulls the connecting rope 27 to pull the counterweight block 28 to lean against the transition box 5, no pressure is generated outwards, and when the electromagnet 17 is electrified, magnetic attraction is generated on the second magnetic block 26 to enable the inner sliding seat 24 to move outwards, the counterweight block 28 is loosened and falls on the bearing plate 23, so that the gravity borne by the gun head pipe 8 is increased, and the vertical state is easier to keep;
the driving structure comprises a back plate 18 fixedly arranged in the transition box 5, a telescopic rod 19 is fixedly arranged on the back plate 18, a movable rod 20 is fixedly connected onto the telescopic rod 19, a push rod 21 is fixedly arranged on the movable rod 20, and the push rod 21 is slidably arranged in a guide rail 22;
the driving structure can work when the level sensor 9 detects that self-modification can be carried out, on one hand, the extrusion on the bent pipe 6 is released, so that the connector 7 has a moving space, and on the other hand, the rope structure is driven;
the back plate 18 is vertically connected to the inner wall of the transition box 5, the telescopic rod 19 is an electric telescopic rod, the telescopic rod 19 is electrically connected with the horizontal sensor 9, the movable rod 20 is fixedly connected to the end of the telescopic rod 19, the push rod 21 is vertically connected with the movable rod 20, the push rod 21 is in contact connection with the bent pipe 6, and the guide rail 22 is fixedly mounted on the inner wall of the transition box 5;
in the driving structure, when the movable rod 20 is at the initial position, the pushing rod 21 can generate pressure to act on the bent pipe 6, so that the bent pipe 6 is kept in a bent state, the connector 7 retracts and is fixed, and when the telescopic rod 19 is electrified, the movable rod 20 can be pulled back, the extrusion on the bent pipe 6 is released, and the bent pipe 6 enters a suspension state;
the rope structure comprises a pull rope 29 fixedly connected with the driving structure, the pull rope 29 is fixedly connected to a labor-consuming lever 30, an unlocking rope 31 is fixedly connected to the labor-consuming lever 30, the unlocking rope 31 is movably connected to a steering rod 32, and the unlocking rope 31 is connected to a rope sleeve 33 in a penetrating mode;
the driving structure drives the rope structure to act on the locking piece 34, so that the locking piece 34 is rotated to be opened through the position at the bottom of the adjusting knob 3, the downward pressing of the adjusting knob 3 is not blocked, and the liquid-transferring gun can perform normal liquid taking work;
the labor-consuming lever 30 is installed in the transition box 5 through a rotating shaft, the pull rope 29 is connected with the short force arm of the labor-consuming lever 30, the unlocking rope 31 is connected with the long force arm of the labor-consuming lever 30, the steering rod 32 is fixedly connected to the inner wall of the transition box 5, the rope sleeve 33 is fixedly installed in the gun body 1, the locking piece 34 is installed on the side surface of the adjusting knob 3 through the torsion spring shaft 35, and the unlocking rope 31 is connected with the end part of the locking piece 34;
specifically, the pull back of the movable rod 20 acts on the pull rope 29, a pulling force is generated to act on the unlocking rope 31 through the displacement amplification effect of the labor-consuming lever 30, the unlocking rope 31 finally pulls the locking piece 34 through the guiding effect of the steering rod 32 and the rope sleeve 33, so that the pipette is unlocked, an operator can smoothly and safely complete pipette operation, the accuracy of pipette values is ensured, and the pipette is prevented from being damaged;
the transition box 5 is fixedly arranged at the bottom of the gun body 1, the bent pipe 6 is communicated with an air cavity of the gun body 1, the connector 7 is connected to the bottom end of the bent pipe 6, the gun head pipe 8 is clamped with the connector 7, the horizontal sensor 9 is arranged at the central position of the transition box 5, and the alarm 10 is an acoustic alarm;
the liquid transfer gun head pipe 8 of the invention is a detachable and replaceable structure, when in use, the gun head pipe 8 is connected with a connector 7, so that a gun body 1, a bent pipe 6 and the gun head pipe 8 are communicated into a complete piston type structure, positive and negative pressure can be generated by controlling a piston rod 2 through an adjusting knob 3, the absorption and transfer work of an exosome extracting solution is completed, the volume of the liquid transfer gun can be adjusted through the rotation of the adjusting knob 3, the volume can also be displayed through a volume display 4, the structure is similar to that of a conventional liquid transfer gun, but a transition box 5 is used as a transition connection structure of the gun body 1 and the gun head pipe 8, the bent pipe 6 is arranged in the transition box 5 and is connected between the gun body 1 and the gun head pipe 8, the length of the bent pipe 6 can be discharged when necessary, the suspension type connection of the gun head pipe 8 is carried out, so that the gun head pipe 8 is automatically suspended and kept in a vertical state under the action of gravity, the operation error can be reduced to a certain degree, an operator can perform perfect operation only by roughly ensuring that the pipette is roughly vertical, the operation difficulty of the pipette is reduced, the pipette is not easy to damage, the operator can confirm whether the operation can be performed or not by listening to the sound of the alarm 10, the levelness sensor 9 arranged in the transition box 5 can perform levelness monitoring, when the verticality of the pipette is within an acceptable range, the alarm 10 stops alarming, and when the levelness sensor 9 detects that the verticality of the gun body 1 is acceptable and modification is difficult to perform through adjustment, the gun head locking structure and the gun head counterweight structure can be further driven, so that the gun head pipe 8 is suspended, and the self-modification effect is achieved;
the working principle is as follows: firstly, the pipette head tube 8 of the pipette is a detachable structure, the pipette head tube 8 is connected with the connector 7 when in use, so that the pipette body 1, the bent tube 6 and the pipette head tube 8 are communicated to form a complete piston structure, positive and negative pressure can be generated by controlling the piston rod 2 through the adjusting knob 3 to finish the suction and transfer work of an exosome extracting solution, the volume of the pipette can be adjusted through the rotation of the adjusting knob 3, the volume can also be displayed through the volume display 4, the structure is similar to that of a conventional pipette, but the transitional box 5 is used as a transitional connection structure of the pipette body 1 and the pipette head tube 8, the bent tube 6 is arranged in the transitional box 5 and is connected between the pipette body 1 and the pipette head tube 8, the length of the bent tube 6 can be discharged when necessary, and the suspended connection of the pipette head tube 8 is carried out, so that the pipette head tube 8 is under the action of gravity, the automatic hanging down keeps the vertical state, can reduce the operation error to a certain extent, the operator only needs to roughly ensure the approximate vertical state of the pipette, the perfect operation can be carried out, the operation difficulty of the pipette is reduced, and the pipette is not easy to damage, the operator can confirm whether the operation can be carried out or not by listening to the sound of the alarm 10, the levelness sensor 9 arranged in the transition box 5 can carry out levelness monitoring, when the verticality of the pipette is in an acceptable range, the alarm 10 stops alarming, and the levelness sensor 9 detects that the verticality of the gun body 1 is acceptable, and when the modification is difficult to be carried out through adjustment, the further driving of a gun head locking structure and a gun head counterweight structure can be carried out, so that the gun head pipe 8 is hung, the self-modification effect is achieved, the invention utilizes the gun head locking structure to fix the connector 7, therefore, the gun head pipe 8 can be fixedly connected to the gun body 1 under normal conditions, the positioning block 15 is pushed by the elastic force of the first spring 14, the positioning block 15 protrudes out of the mounting box 13 and is connected with the positioning hole 12 on the side plate 11, the gun head locking structure is provided with a left part and a right part, the connector 7 can be stably positioned in the transition box 5 and is connected with the gun head pipe 8, when the level sensor 9 detects that the gun head pipe 8 has a fine adjustment space, the electromagnet 17 can be electrified, the first magnetic block 16 is subjected to magnetic attraction, the positioning block 15 retracts, the connector 7 is unlocked, the connector 7 has mobility, the weight of the gun head pipe 8 is light, although the mobility is obtained, the suspension can be carried out, the verticality is difficult to keep, therefore, a gun head counterweight structure is further arranged, and pressure cannot be caused on the gun head pipe 8 under normal conditions, when self-modification is carried out, the gun head pipe 8 is pressed down in a balanced manner, generally, the elastic force of the second spring 25 acts on the inner sliding seat 24, the inner sliding seat 24 pulls the connecting rope 27 to pull the balancing weight 28 to lean against the transition box 5, and no pressure is generated outwards, when the electromagnet 17 is electrified, magnetic attraction is generated on the second magnetic block 26, so that the inner sliding seat 24 moves outwards, the balancing weight 28 is loosened and falls on the bearing plate 23, the gravity borne by the gun head pipe 8 is increased, the vertical state is easier to keep, the driving structure can work when the horizontal sensor 9 detects that self-modification can be carried out, on one hand, the extrusion on the bent pipe 6 is released, so that the connector 7 has a movable space, on the other hand, the rope structure is driven, in the driving structure, when the movable rod 20 is in the initial position, the push rod 21 can generate pressure to act on the bent pipe 6, so that the bent pipe 6 is kept in a bending state, the connector 7 retracts and is fixed, when the telescopic rod 19 is electrified, the movable rod 20 can be pulled back, the extrusion on the bent pipe 6 is released, the bent pipe 6 enters a suspension state, the driving structure drives the rope structure, the rope structure can act on the locking piece 34, the locking piece 34 can be rotated to be opened through the position at the bottom of the adjusting knob 3, the pressing-down of the adjusting knob 3 is not blocked, the normal liquid taking work of the liquid transferring gun can be shown, specifically, the pulling-back of the movable rod 20 acts on the pull rope 29, the pulling force is generated to act on the unlocking rope 31 through the displacement amplification effect of the labor-consuming lever 30, the unlocking rope 31 finally pulls the locking piece 34 through the guiding effect of the steering rod 32 and the rope sleeve 33, the liquid transferring gun is unlocked, an operator can smoothly and safely complete the liquid transferring operation, and the accurate numerical value is ensured, and prevents damage to the pipette.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The utility model provides an extract pipette of exosome production usefulness, includes body of a gun (1), its characterized in that: the gun body (1) is movably connected with a piston rod (2), the piston rod (2) is rotatably provided with an adjusting knob (3), the gun body (1) is fixedly provided with a volume display (4), the gun body (1) is fixedly connected with a transition box (5), the transition box (5) is fixedly connected with a bent pipe (6), the bent pipe (6) is fixedly connected with a connector (7), the connector (7) is fixedly connected with a gun head pipe (8), the transition box (5) is fixedly provided with a level sensor (9), the level sensor (9) is electrically connected with an alarm (10), the connector (7) is fixedly provided with a side plate (11), the side plate (11) is provided with a positioning hole (12), and the transition box (5) is internally provided with a gun head locking structure with an electromagnetic unlocking function and an electromagnet (17), and install the rifle head counter weight structure that has the electromagnetism unblock function in transition box (5), and fixed mounting has loading board (23) on rifle head pipe (8), install the drive structure that has unbalance and start in transition box (5), and the structural rope structure that is connected with the displacement and enlargies the function of drive, and rope structural connection is on locking piece (34) of going on installing through torsional spring axle (35) and body of a gun (1).
2. An extract pipetting gun for exosome production according to claim 1, characterized in that: the gun head locking structure comprises a mounting box (13) fixedly mounted in a transition box (5), a first spring (14) is fixedly mounted in the mounting box (13), a positioning block (15) is fixedly connected to the first spring (14), a first magnetic block (16) is fixedly mounted on the positioning block (15), the mounting box (13) is located on the side face of a side plate (11), the positioning block (15) is slidably mounted in the mounting box (13), the end portion of the positioning block (15) penetrates through the side face of the mounting box (13), and the first magnetic block (16) is fixedly mounted at the top of the positioning block (15).
3. An extract pipetting gun for exosome production according to claim 2, characterized in that: the gun head balance weight structure and the gun head locking structure are respectively located on two sides of the electromagnet (17), the gun head balance weight structure comprises an inner sliding seat (24) which is slidably mounted in the transition box (5), a second spring (25) and a second magnetic block (26) are fixedly connected to the inner sliding seat (24), a connecting rope (27) is fixedly connected to the inner sliding seat (24), and a balancing weight (28) is fixedly connected to the connecting rope (27).
4. An extract pipette for exosome production according to claim 3, characterised in that: second spring (25) fixedly connected with is between transition box (5) inner wall and interior slide (24), and second magnetic path (26) and first magnetic path (16) adopt the opposite installation of magnetic pole orientation, electro-magnet (17) are located the middle part of first magnetic path (16) and second magnetic path (26), the bottom of transition box (5) is provided with the recess, and balancing weight (28) are located the recess, interior slide (24) and balancing weight (28) are located the box of transition box (5) respectively with the box outside, and connect rope (27) and connect between the two.
5. An extract pipetting gun for exosome production according to claim 1, characterized in that: the drive structure is including backplate (18) of fixed mounting in transition box (5), and fixed mounting has telescopic link (19) on backplate (18), fixedly connected with movable rod (20) on telescopic link (19), and fixed mounting has catch bar (21) on movable rod (20), catch bar (21) slidable mounting is in guided way (22).
6. An extract pipetting gun as recited in claim 5 for exosome production comprising: backplate (18) are connected perpendicularly on the inner wall of transition box (5), and telescopic link (19) adopt electric telescopic handle, and telescopic link (19) and level sensor (9) electric connection, movable rod (20) fixed connection is at the tip of telescopic link (19), and catch bar (21) and movable rod (20) are connected perpendicularly, catch bar (21) and return bend (6) contact are connected, and guided way (22) fixed mounting is on the inner wall of transition box (5).
7. An extract pipetting gun for exosome production according to claim 1, characterized in that: the rope structure is including stay cord (29) with drive structure fixed connection, and stay cord (29) fixed connection is on hard lever (30), fixed connection has unlocking rope (31) on hard lever (30), and unlocking rope (31) swing joint is on steering column (32), and unlocking rope (31) through connection is in fag end (33).
8. An extract pipetting gun as recited in claim 7 for exosome production comprising: hard lever (30) are installed in transition box (5) through the pivot, and stay cord (29) are connected with the short arm of force of hard lever (30), and unlock rope (31) and the long arm of force of hard lever (30) are connected, steering bar (32) fixed connection is on the inner wall of transition box (5), and noose (33) fixed mounting in body of a gun (1), the side at adjust knob (3) is installed through torsional spring axle (35) in locking piece (34), and the end connection of unlocking rope (31) and locking piece (34).
9. An extract pipetting gun for exosome production according to claim 1, characterized in that: transition box (5) fixed mounting is in the bottom of body of a gun (1), and return bend (6) and the air cavity intercommunication of body of a gun (1), the bottom at return bend (6) is connected in connector (7), and rifle head pipe (8) and connector (7) joint, the central point that level sensor (9) were installed in transition box (5) puts, and alarm (10) are audible alarm.
CN202111411869.1A 2021-11-24 2021-11-24 Extracting solution liquid-transfering gun of exosome production usefulness Active CN113908901B (en)

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