CN113897314B - Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method - Google Patents
Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method Download PDFInfo
- Publication number
- CN113897314B CN113897314B CN202111206808.1A CN202111206808A CN113897314B CN 113897314 B CN113897314 B CN 113897314B CN 202111206808 A CN202111206808 A CN 202111206808A CN 113897314 B CN113897314 B CN 113897314B
- Authority
- CN
- China
- Prior art keywords
- amycolatopsis
- chloropropyl
- phosphate
- tri
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/22—Organic substances containing halogen
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
Abstract
The invention discloses a method for degrading tri (2-chloropropyl) phosphate by using Amycolatopsis sp.FT-1 and application thereof, belonging to the technical field of biological treatment of environmental organic pollutants. The method comprises the following specific steps: the degradation effect of Amycolatopsis (Amycolatopsis sp. FT-1) on the tri (2-chloropropyl) phosphate is analyzed by inoculating Amycolatopsis (Amycolatopsis sp. FT-1) into a degradation medium containing tri (2-chloropropyl) phosphate at 30 ℃ and measuring the residual concentration of tri (2-chloropropyl) phosphate in the medium by using a gas chromatography mass spectrometry (GC-MS) after 5d degradation by a constant temperature shaking table. The method has strong environmental adaptability and lower cost, the degradation rate of the tri (2-chloropropyl) phosphate with the initial concentration of 5mg/L can reach 85.84 percent after 5d degradation, and the practical application value is high, thereby providing a reference for solving the problem of the pollution treatment of the tri (2-chloropropyl) phosphate in the environment.
Description
Technical Field
The invention belongs to the technical field of biological treatment of environmental organic pollutants, and particularly relates to a method for degrading tri (2-chloropropyl) phosphate by using Amycolatopsis sp.FT-1 and application thereof.
Background
In recent years, along with the gradual withdrawal of the traditional brominated flame retardant from the flame retardant market due to the defects of large smoke generation amount, strong corrosiveness, easy release of toxic gas and the like, the novel organophosphorus flame retardant is widely applied to the production of various commercial products such as textiles, electronic products, plastics, furniture, coatings and the like as an ideal substitute of the brominated flame retardant. The tri (2-chloropropyl) phosphate is the most common organic phosphorus flame retardant at present, and is added into various products in a physical mixing mode such as doping, adsorption and the like due to the addition of chemical auxiliary agents, and is not organically combined with the products in a chemical bonding mode, so that the tri (2-chloropropyl) phosphate is easily released from the products in a way of volatilization, abrasion, corrosion, leakage and the like to enter natural environment, and environmental pollution is caused along with the migration of the atmosphere and water body. At present, the presence of tri (2-chloropropyl) phosphate can be detected in various environmental media such as airborne dust, water bodies, soil, river sediment, sediments and the like. The tri (2-chloropropyl) phosphate remained in the organism can produce various toxic effects on endocrine disruptors, reproductive toxicity, neurotoxicity, immune toxicity and the like. As a new class of organic contaminants, the ecological risks and health safety effects of this organophosphorus flame retardant in the environment have attracted considerable attention from environmental researchers.
At present, the degradation and removal of the organophosphorus flame retardant mainly adopts a physicochemical method and a microbial degradation method. The physicochemical method cannot be the best choice for pollution control because of the problems of high operation cost, easy secondary pollution and the like. The microbial degradation method has the advantages of environmental friendliness and low cost, so that the microbial degradation method becomes one of the safest, economical and effective organic pollution restoration methods, but the microbial degradation of the tri (2-chloropropyl) phosphate is limited due to the lack of effective degradation strains, so that the screening and application of the efficient degradation bacteria of the tri (2-chloropropyl) phosphate have a certain research significance. There is no patent related to the degradation of tri (2-chloropropyl) phosphate by bacterial species in the present state of the art.
Disclosure of Invention
The primary aim of the invention is to provide a method for degrading the tri (2-chloropropyl) phosphate by adopting Amycolatopsis sp.FT-1 and application thereof, and a technical method is provided for pollution control and bioremediation of the Amycolatopsis sp.FT-1 by adopting the rapid and efficient degradation of the tri (2-chloropropyl) phosphate by the Amycolatopsis sp.FT-1.
The aim of the invention is achieved by the following technical scheme.
A method for degrading tri (2-chloropropyl) phosphate by using Amycolatopsis sp.FT-1 and application thereof, comprising the following steps:
(1) Amycolatopsis sp.FT-1 is inoculated into sterilized enrichment medium and shake cultured in a shaker.
(2) The strain is collected and then inoculated into sterilized tri (2-chloropropyl) phosphate degradation medium for shaking culture.
(3) The degradation effect of Amycolatopsis (Amycolatopsis sp.FT-1) on the tri (2-chloropropyl) phosphate in the degradation medium is analyzed by a gas chromatography mass spectrometer.
Preferably, the enrichment medium of step (1) comprises the following components: 20g/L glucose, 3g/L KH 2 PO 4 ，1.5g/L MgSO 4 Thiamine hydrochloride 0.008g/L, 1000mL of potato extract, and pH 6.0.
Preferably, the potato extract liquid in the step (1) is prepared by the following method: 200g of peeled potatoes are taken, cut into small pieces, 500mL of ultrapure water is added, boiling is carried out for 30min, the potato pieces are crushed, then double-layer gauze is used for filtering to remove potato residues, and the obtained filtrate is complemented to 1000mL.
The culture conditions in the step (1) are as follows: the temperature is 20-40 ℃, the rotating speed is 150-170rpm, and the constant-temperature shaking culture time is 84-108 h; preferred culture conditions are: the temperature is 30 ℃, the rotating speed is 160rpm, and the culture time is 96 hours.
The composition of the tri (2-chloropropyl) phosphate degradation medium in the step (2) is as follows: 2g/L (NH) 4 ) 2 SO 4 ，1.5g/L Na 2 HPO 4 ·12H 2 O，1.5g/L K 2 HPO 4 ，0.2g/L MgSO 4 ·7H 2 O，0.01g/L CaCl 2 ，3-10g/L glucose, 5-20 mg/L tri (2-chloropropyl) phosphate, 0.1mL/L microelement solution, 1000mL ultrapure water, and the pH is 4.0-9.0. Wherein the microelement solution comprises the following components: 50g/L FeSO 4 ·7H 2 O，2.2g/L ZnSO 4 ·7H 2 O，0.3g/L CuSO 4 ·5H 2 O,14.3g/L MnSO 4 ·H 2 O,1000mL of ultrapure water. The preferred glucose concentration is 5g/L and the preferred pH is 7.0.
The culture conditions in the step (2) are as follows: the inoculation amount is 2-4g/L, the temperature is 20-40 ℃, the rotating speed is 150-170rpm, and the culture time is 3-5d. Preferred culture conditions are: the inoculation amount is 3g/L, the temperature is 30 ℃, the rotating speed is 160rpm, and the culture time is 5d.
The Amycolatopsis (Amycolatopsis sp.FT-1) is screened from activated sludge of a Hund sewage treatment plant in Guangzhou, the strain is domesticated by adopting a method for increasing pollutants in a gradient manner, tri (2-chloropropyl) phosphate is added into an inorganic salt culture medium, a domestication culture medium with the concentration gradient of 5, 10, 20, 30, 50, 70 and 100mg/L of tri (2-chloropropyl) phosphate is prepared, and the domestication is performed by adopting a domestication method for increasing the concentration of the pollutants in a gradient manner. The bacteria are amycolatopsis, the external form is observed, the bacteria are dry and wrinkled, the surface is a white circular colony with raised edges and concave middle parts and is irregularly and non-transparent.
The Amycolatopsis (Amycolatopsis sp.FT-1) is deposited in the Guangdong province microorganism strain collection with the deposit number of GDMCC No:61664, date of preservation: 2021, 5-12, deposit address: guangzhou city first middle road No. 100 college No. 59 building 5.
Under the condition, the degradation culture is carried out for 5 days, and the degradation rate of the tri (2-chloropropyl) phosphate with the initial concentration of 5mg/L reaches 85.84 percent.
Compared with the prior art, the invention has the following advantages and technical effects:
1. the invention provides a method for degrading tri (2-chloropropyl) phosphate by adopting Amycolatopsis sp.FT-1 and application thereof, and the method can provide a technical method for pollution control and bioremediation of the tri (2-chloropropyl) phosphate by adopting the Amycolatopsis sp.FT-1.
2. The amycolatopsis in the invention has strong environmental adaptability, good degradation effect on the tri (2-chloropropyl) phosphate, and low cost for degrading the tri (2-chloropropyl) phosphate by using the amycolatopsis.
Drawings
FIG. 1 is a plot of tris (2-chloropropyl) phosphate versus peak area standard;
FIG. 2 is a graph of degradation of tris (2-chloropropyl) phosphate by Amycolatopsis sp. FT-1 at various times.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but embodiments of the present invention are not limited thereto.
Example 1
1. Screening of degradation strains
(1) Activated sludge of a Hund sewage treatment plant in Guangzhou city is collected, 100mL of activated sludge is added into 250mL of inorganic salt culture medium containing 5mg/L tris (2-chloropropyl) phosphate, and the mixture is cultured in a constant temperature shaking table at 30 ℃ and 160 rpm. The main components of the inorganic salt culture medium are as follows: 2g/L (NH) 4 ) 2 SO 4 ，1.5g/L Na 2 HPO 4 ·12H 2 O,1.5g/L K 2 HPO 4 ，0.2g/L MgSO 4 ·7H 2 O，0.01g/L CaCl 2 0.1mL/L trace element solution, 1000mL of ultra pure water, pH 7.0. Wherein the microelement solution comprises the following components: 50g/L FeSO 4 ·7H 2 O，2.2g/L ZnSO 4 ·7H 2 O，0.3g/L CuSO 4 ·5H 2 O，14.3g/L MnSO 4 ·H 2 O,1000mL of ultrapure water.
(2) Transferring 1 time every 10 days, increasing the concentration of tri (2-chloropropyl) phosphate (according to concentration gradient of 5, 10, 20, 30, 50, 70 and 100 mg/L) while transferring until the concentration of tri (2-chloropropyl) phosphate reaches 100mg/L, separating and purifying strain by dilution gradient method and plate coating method, selecting colony with rapid growth and different forms, ensuring pure single strain, inoculating onto inorganic salt solid medium containing 0.5mg of tri (2-chloropropyl) phosphate, and preserving at 4deg.CIn the refrigerator, degradation experiments are to be carried out later. The inorganic salt solid culture medium comprises the following components: 2g/L (NH) 4 ) 2 SO 4 ，1.5g/L Na 2 HPO 4 ·12H 2 O， 1.5g/L K 2 HPO 4 ，0.2g/L MgSO 4 ·7H 2 O，0.01g/L CaCl 2 18g/L agar, 0.1mL/L trace element solution, 1000mL ultra pure water, and pH 7.0. Wherein the microelement solution comprises the following components: 50g/L FeSO 4 ·7H 2 O，2.2g/L ZnSO 4 ·7H 2 O，0.3 g/L CuSO 4 ·5H 2 O，14.3g/L MnSO 4 ·H 2 O,1000mL of ultrapure water.
2. Identification of degradation strains
(1) Thallus and colony morphological characteristics
The bacteria are amycolatopsis, the external form is observed, the bacteria are dry and wrinkled, the surface is a white circular colony with raised edges and concave middle parts and is irregularly and non-transparent.
(2) ITS rDNA sequences
The bacterial sequence has 99.38% homology with the ITS rDNA sequence of Amycolatopsis by comparison of ITS rDNA sequence determination and analysis, the strain is determined to be Amycolatopsis (Amycolatopsis sp.) and the ITS rDNA sequence is named Amycolatopsis sp.FT-1 as shown in a sequence table 1, wherein the ITS rDNA sequence (1458 bp in total) is submitted to gene bank with the accession number MZ040131. The strain can be obtained from the collection of microorganism strains in Guangdong province, and the collection number is GDMCC No:61664.
EXAMPLE 2 analysis of degradation of tris (2-chloropropyl) phosphate by Amycolatopsis
Amycolatopsis sp.FT-1 was inoculated into sterilized enrichment medium and cultured at 30℃with shaking at 160rpm for 96h. The main components of the enrichment medium are as follows: 20g/L glucose, 3g/L KH 2 PO 4 ，1.5g/L MgSO 4 Thiamine hydrochloride 0.008g/L, 1000mL of potato extract, and pH 6.0.
The potato extract is prepared by the following steps: 200g of peeled potatoes are taken, cut into small pieces, 500mL of ultrapure water is added, boiling is carried out for 30min, the potato pieces are crushed, then double-layer gauze is used for filtering to remove potato residues, and the obtained filtrate is complemented to 1000mL.
Centrifuging the enriched bacterial liquid at 3000rpm for 1min, collecting bacterial cells, repeatedly washing with a degradation culture medium for 3 times, adding the degradation culture medium to prepare bacterial suspension with the concentration of 100g/L, adding 600 mu L of the bacterial suspension into 19.4mL of sterilized degradation culture medium (namely, the inoculation amount is 3 g/L), performing degradation experiments, and after shaking at a constant temperature of 30 ℃ and 160rpm for 5d, determining the degradation effect of the bacterial cells on the tri (2-chloropropyl) phosphate by GC-MS (gas chromatography-mass spectrometry). The degradation culture medium comprises the following components: 2g/L (NH) 4 ) 2 SO 4 ，1.5g/L Na 2 HPO 4 ·12H 2 O，1.5g/L K 2 HPO 4 ，0.2g/L MgSO 4 ·7H 2 O，0.01g/L CaCl 2 5. 5g/L glucose, 5mg/L tris (2-chloropropyl) phosphate, 0.1mL/L trace element solution, 1000mL ultrapure water, and pH 7.0. Wherein the microelement solution comprises the following components: 50g/L FeSO 4 ·7H 2 O，2.2g/L ZnSO 4 ·7H 2 O，0.3g/L CuSO 4 ·5H 2 O，14.3 g/L MnSO 4 ·H 2 O,1000mL of ultrapure water.
The concentration of tris (2-chloropropyl) phosphate was determined by GC-MS under the following analytical conditions:
(1) Gas chromatography conditions
Chromatographic column: SH-Rxi-5Sil MS (30 m×0.25mm×0.25 μm) capillary column, carrier gas is ultra-high purity helium, constant flow mode, column flow is 1.0mL/min, sample injection is not split, sample injection amount is 1uL, and sample inlet temperature is 280 ℃; the temperature program was 50℃for 1min at initial temperature, 15℃/min to 200℃for 1min, and 4℃/min to 220℃for 1min.
(2) Mass spectrometry conditions: ion source temperature 230 ℃, injector temperature 280 ℃; the ion source is EI source, and the solvent delay time is 3min.
Standard solutions with the concentrations of the tri (2-chloropropyl) phosphate of 0.1, 0.3, 0.5, 1.0, 3.0 and 5.0mg/L are prepared, and a tri (2-chloropropyl) phosphate-peak area standard curve is drawn, wherein the standard curve is shown in figure 1. The final residual concentration of tris (2-chloropropyl) phosphate in the degradation medium of the control group was determined to be 4.980 mg/L after 5d degradation by inoculating inactivated Amycolatopsis sp.FT-1 as the control group (the control group was the same as the experimental group except that the inoculated seed was inactivated), and the final residual concentration of triphenyl phosphate in the degradation medium of the experimental group was 0.705mg/L, which was obtained by comparing the control group and the experimental group (the method provided in example 2), the degradation efficiency of the experimental group was 85.84%, and the results are shown in FIG. 2.
Example 3
Example 3 As in example 2, except that the glucose concentration in the degradation medium was 3g/L and 10g/L, respectively, the degradation rate of the final Amycolatopsis (Amycolatopsis sp. FT-1) on tris (2-chloropropyl) phosphate was 28.30% and 88.76%, respectively.
Example 4
Example 4 As in example 2, except that the initial concentration of tris (2-chloropropyl) phosphate in the degradation medium was 10mg/L and 20mg/L, respectively, the degradation rate of tris (2-chloropropyl) phosphate by the final Amycolatopsis sp. FT-1 was 85.22% and 84.05%, respectively.
Example 5
Example 5 As in example 2, except that during the degradation, the pH of the degradation medium was 4, 5, 6, 8 and 9, respectively, the degradation rate of the final Amycolatopsis sp. FT-1 to tris (2-chloropropyl) phosphate was 67.71%, respectively,
65.85%, 77.06%, 54.22% and 52.04%.
Example 6
Example 6 was conducted in the same manner as in example 2 except that the inoculum size was 2g/L and 4g/L, respectively, and the degradation rates of the final Amycolatopsis sp. FT-1 on tris (2-chloropropyl) phosphate were 69.37% and 88.72%, respectively.
Example 7
Example 7 As in example 2, except that the temperature was 20℃and the rotational speed was 150rpm during the enrichment and degradation, the degradation rate of tris (2-chloropropyl) phosphate by the final Amycolatopsis sp. FT-1 was 53.62%.
Example 8
Example 8 As in example 2, except that during the enrichment and degradation process, the temperature was 40℃and the rotational speed was 170rpm, the final degradation rate of tris (2-chloropropyl) phosphate by Amycolatopsis sp. FT-1 was 77.93%.
Example 9
Example 9 was conducted in the same manner as in example 2 except that the enrichment culture time was 84h and 108h, respectively, and the degradation rate of tris (2-chloropropyl) phosphate by Amycolatopsis sp. FT-1 was 80.45% and 73.29% respectively.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Sequence listing
<110> university of Guilin theory
<120> a method for degrading tris (2-chloropropyl) phosphate using amycolatopsis and use thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1458
<212> DNA
<213> Amycolatopsis halotolerans
<400> 1
aggctcagga cgaacgctgg cggcgtgctt aacacatgca agtcgaacgc tgaaccggtt 60
tcggccgggg atgagtggcg aacgggtgag taacacgtgg gtaatctgcc ctgcactctg 120
ggataagcct gggaaactgg gtctaatacc ggatatgacc actggaggca tctttggtgg 180
tggaaagttc cggcggtgca ggatgaaccc gcggcctatc agcttgttgg tggggtaatg 240
gcctaccaag gcgacgacgg gtagccggcc tgagagggtg accggccaca ctgggactga 300
gacacggccc agactcctac gggaggcagc agtggggaat attgcacaat gggcgcaagc 360
ctgatgcagc gacgccgcgt gagggatgac ggccttcggg ttgtaaacct ctttcgccag 420
ggacgaagcg caagtgacgg tacctggata agaagcaccg gctaactacg tgccagcagc 480
cgcggtaata cgtagggtgc gagcgttgtc cggaattatt gggcgtaaag agctcgtagg 540
cggtttgtcg cgtcggccgt gaaatctcca cgcttaacgt ggagcgtgcg gtcgatacgg 600
gcagacttga gttcggcagg ggagactgga attcctggtg tagcggtgaa atgcgcagat 660
atcaggagga acaccggtgg cgaaggcggg tctctgggcc gatactgacg ctgaggagcg 720
aaagcgtggg gagcgaacag gattagatac cctggtagtc cacgctgtaa acgttgggcg 780
ctaggtgtgg gcgacatcca cgttgtccgt gccgtagcta acgcattaag cgccccgcct 840
ggggagtacg gccgcaaggc taaaactcaa aggaattgac gggggcccgc acaagcggcg 900
gagcatgtgg attaattcga tgcaacgcga agaaccttac ctgggcttga catgcgccag 960
acatccccag agatggggct tcccttgtgg ttggtgtaca ggtggtgcat ggctgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctgcgtt 1080
gccagcgcgt catggcgggg actcgtggga gactgccggg gtcaactcgg aggaaggtgg 1140
ggatgacgtc aagtcatcat gccccttatg tccagggctt cacacatgct acaatggctg 1200
gtacagaggg ctgcgatacc gcgaggtgga gcgaatccct taaagccggt ctcagttcgg 1260
atcgcagtct gcaactcgac tgcgtgaagt cggagtcgct agtaatcgca gatcagcaac 1320
gctgcggtga atacgttccc gggccttgta cacaccgccc gtcacgtcat gaaagtcggt 1380
aacacccgaa gcccatggcc caacccgcaa gggagggagt ggtcgaaggt gggactggcg 1440
attgggacga agtcgtat 1458
Claims (6)
1. An amycolatopsis is adoptedAmycolatopsis sp. FT-1) a process for degrading tris (2-chloropropyl) phosphate, characterized in that it comprises the following steps:
(1) The amycolatopsis is treatedAmycolatopsissp, FT-1) inoculating into sterilized enrichment medium, and shake culturing in a shaker;
(2) Collecting strains, and then inoculating the strains into sterilized tris (2-chloropropyl) phosphate degradation culture medium for shake culture;
(3) The tri (2-chloropropyl) phosphate in the degradation culture medium is measured by a gas chromatography mass spectrometer and analyzed to obtain the amycolatopsis @ of the amycolatopsis @Amycolatopsissp, FT-1) degradation effect on tris (2-chloropropyl) phosphate;
the amycolatopsis isAmycolatopsis sp.FT-1) deposited with the microorganism strain collection, guangdong province under the accession number GDMCC No:61664.
2. a method according to claim 1, wherein the method comprises the step ofAmycolatopsissp, FT-1) method for degrading tri (2-chloropropyl) phosphate, which is characterized in that: the enrichment medium in the step (1) comprises the following components: 20g/L glucose, 3g/L KH 2 PO 4 ，1.5 g/L MgSO 4 Thiamine hydrochloride 0.008g/L, potato extract 1000mL, pH 6.0.
3. A method according to claim 1, wherein the method comprises the step ofAmycolatopsissp, FT-1) method for degrading tri (2-chloropropyl) phosphate, which is characterized in that: the culture conditions in the step (1) are as follows: the temperature was 30℃and the rotational speed was 160rpm, and the incubation time was 96h.
4. A method according to claim 1, wherein the method comprises the step ofAmycolatopsissp, FT-1) method for degrading tri (2-chloropropyl) phosphate, which is characterized in that: the composition of the tri (2-chloropropyl) phosphate degradation medium in the step (2) is as follows: 2g/L (NH) 4 ) 2 SO 4 ，1.5 g/L Na 2 HPO 4 ·12H 2 O，1.5 g/L K 2 HPO 4 ，0.2 g/L MgSO 4 ·7H 2 O，0.01 g/L CaCl 2 5. 5g/L glucose, 5. 5mg/L tris (2-chloropropyl) phosphate, 0.1. 0.1mL/L trace element solution, 1000. 1000mL ultrapure water, and a pH of 7.0; wherein the microelement solution comprises the following components: 50g/L FeSO 4 ·7H 2 O，2.2 g/L ZnSO 4 ·7H 2 O，0.3 g/L CuSO 4 ·5H 2 O，14.3 g/L MnSO 4 ·H 2 O,1000mL ultrapure water.
5. A method according to claim 1, wherein the method comprises the step ofAmycolatopsissp, FT-1) method for degrading tri (2-chloropropyl) phosphate, which is characterized in that: the culture conditions in the step (2) are as follows: the inoculation amount is 3g/L, the temperature is 30 ℃, the rotating speed is 160rpm, and the culture time is 5d.
6. A method according to any one of claims 1 to 5Amycolatopsissp, FT-1) the application of the method for degrading the tri (2-chloropropyl) phosphate in the aspect of bioremediation of the organic phosphorus flame retardant pollution;
the organophosphorus flame retardant is tri (2-chloropropyl) phosphate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111206808.1A CN113897314B (en) | 2021-10-15 | 2021-10-15 | Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111206808.1A CN113897314B (en) | 2021-10-15 | 2021-10-15 | Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113897314A CN113897314A (en) | 2022-01-07 |
| CN113897314B true CN113897314B (en) | 2023-08-11 |
Family
ID=79192557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111206808.1A Active CN113897314B (en) | 2021-10-15 | 2021-10-15 | Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113897314B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851515A (en) * | 2022-10-26 | 2023-03-28 | 广东工业大学 | Zavarroa capable of degrading organophosphorus flame retardant and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261568A (en) * | 2014-08-29 | 2015-01-07 | 东华大学 | Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis |
| CN105420163A (en) * | 2015-12-24 | 2016-03-23 | 太原科技大学 | Dibenzanthracene degrading bacterium and application thereof |
| CN106006993A (en) * | 2016-04-30 | 2016-10-12 | 华南理工大学 | Method for degrading triphenyl phosphate through brevibacillus brevis and application thereof |
| CN106085894A (en) * | 2016-04-14 | 2016-11-09 | 浙江工业大学 | The application in degraded quinestrol of a kind of amycolatosis |
| CN111983069A (en) * | 2020-08-11 | 2020-11-24 | 深圳市环境科学研究院 | Detection method for determining phosphorus flame retardant and degradation product thereof in surface water |
| CN112251362A (en) * | 2020-12-11 | 2021-01-22 | 桂林理工大学 | Aspergillus for degrading triphenyl phosphate and tricresyl phosphate and screening method and application thereof |
| CN113265347A (en) * | 2021-04-02 | 2021-08-17 | 中国科学院广州地球化学研究所 | Yellow bacillus T21 and application thereof |
-
2021
- 2021-10-15 CN CN202111206808.1A patent/CN113897314B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261568A (en) * | 2014-08-29 | 2015-01-07 | 东华大学 | Method of degrading phosphate tri(2-chloroethyl) ester by adopting bacillus thuringiensis |
| CN105420163A (en) * | 2015-12-24 | 2016-03-23 | 太原科技大学 | Dibenzanthracene degrading bacterium and application thereof |
| CN106085894A (en) * | 2016-04-14 | 2016-11-09 | 浙江工业大学 | The application in degraded quinestrol of a kind of amycolatosis |
| CN106006993A (en) * | 2016-04-30 | 2016-10-12 | 华南理工大学 | Method for degrading triphenyl phosphate through brevibacillus brevis and application thereof |
| CN111983069A (en) * | 2020-08-11 | 2020-11-24 | 深圳市环境科学研究院 | Detection method for determining phosphorus flame retardant and degradation product thereof in surface water |
| CN112251362A (en) * | 2020-12-11 | 2021-01-22 | 桂林理工大学 | Aspergillus for degrading triphenyl phosphate and tricresyl phosphate and screening method and application thereof |
| CN113265347A (en) * | 2021-04-02 | 2021-08-17 | 中国科学院广州地球化学研究所 | Yellow bacillus T21 and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《Biodegradation of tricresyl phosphate isomers by Brevibacillus brevis: Degradation pathway and metabolic mechanism》;Ying Liu等;《Chemosphere》;第232卷;第195-203页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113897314A (en) | 2022-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dan et al. | Biodegradation of benzo [a] pyrene in soil by Mucor sp. SF06 and Bacillus sp. SB02 co-immobilized on vermiculite | |
| CN111748483B (en) | Bacillus for degrading petroleum hydrocarbon and application thereof | |
| CN108102979B (en) | Degradation strain JN5 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN106434470B (en) | A kind of polycyclic aromatic hydrocarbon-degrading bacteria and its application | |
| CN110643534B (en) | Phellinus deltoidea capable of degrading triphenyl phosphate | |
| CN111733098B (en) | Application of bacillus in low-temperature degradation of petroleum hydrocarbon | |
| CN102676424B (en) | Obligate halophile for processing high-salt oily waste liquor in petroleum industry | |
| CN108048374B (en) | Degradation strain JN4 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN108034626B (en) | Degradation strain JN1 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN111117993B (en) | Biomass immobilized fungus-bacterium mixed particle for degrading PAHs in soil as well as preparation method and application thereof | |
| CN113897314B (en) | Method for degrading tri (2-chloropropyl) phosphate by adopting amycolatopsis and application of method | |
| CN108102978B (en) | Degradation strain JN8 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN112251362B (en) | Aspergillus for degrading triphenyl phosphate and tricresyl phosphate and screening method and application thereof | |
| CN101972774A (en) | Microbial repair method of oil-polluted wetland | |
| CN108034625B (en) | Degradation strain JN7 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN114045238B (en) | Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application thereof | |
| CN115786179A (en) | Bacterial strain for degrading o-dichlorobenzene and application thereof | |
| CN112899185B (en) | Screening and application of benzo [ a ] anthracene degrading bacteria in black and odorous bottom mud | |
| CN108048376B (en) | Degradation strain JN3 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN108048375B (en) | Degradation strain JN6 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN108102977B (en) | Degradation strain JN2 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN111944719A (en) | Dioxane degrading bacterium IS20 as well as preparation method and application thereof | |
| CN115125158B (en) | Bacterial strain and microbial inoculum for degrading petroleum hydrocarbon and application thereof | |
| CN114107068B (en) | Fungus capable of degrading petroleum hydrocarbon and application of fungus in petroleum degradation | |
| CN116254188B (en) | Strain SRB-6 and application thereof in degrading herbicide acetochlor under anaerobic condition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |