CN113897290A - One-step preparation method and production system of embryo culture solution - Google Patents

One-step preparation method and production system of embryo culture solution Download PDF

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Publication number
CN113897290A
CN113897290A CN202111326231.8A CN202111326231A CN113897290A CN 113897290 A CN113897290 A CN 113897290A CN 202111326231 A CN202111326231 A CN 202111326231A CN 113897290 A CN113897290 A CN 113897290A
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reaction barrel
solution
production system
embryo culture
nutrient solution
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杨五宁
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Nanjing Youer Biotechnology Development Co ltd
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Nanjing Youer Biotechnology Development Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/18—Stationary reactors having moving elements inside
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

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  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of medicines, and particularly discloses a one-step preparation method and a production system of an embryo culture solution. The setting of above structure can be so that carbon dioxide gas is efficient to be contacted with the nutrient solution to the pH value of effectual regulation nutrient solution.

Description

One-step preparation method and production system of embryo culture solution
Technical Field
The invention relates to the technical field of medicines, in particular to a one-step preparation method and a production system of an embryo culture solution.
Background
Currently, the tube infant refers to taking out the ovum and the sperm, putting the ovum and the sperm in a test tube to make the ovum fertilized, then transplanting the embryo precursor, namely the fertilized ovum, back to the mother uterus to develop into a fetus, wherein carbon dioxide is needed to adjust the pH value of the nutrient solution during the preparation of the embryo nutrient solution so as to make the pH value of the nutrient solution reach a proper pH value.
However, the carbon dioxide is introduced into the raw material of the nutrient solution while stirring the raw material, and the reaction efficiency between the nutrient solution and the carbon dioxide is low.
Disclosure of Invention
The invention aims to provide a one-step preparation method and a production system of an embryo culture solution, and aims to solve the technical problem that in the prior art, carbon dioxide is filled in a process of stirring a raw material of a nutrient solution, so that the reaction efficiency of the nutrient solution and the carbon dioxide is low.
In order to achieve the purpose, the one-step production system for the embryo culture solution comprises a reaction barrel, a barrel cover, a support, a motor, a stirring assembly and an air inlet pipe, wherein the barrel cover is fixedly connected with the reaction barrel and covers the reaction barrel, the stirring assembly is rotatably connected with the barrel cover and is positioned in the reaction barrel, the support is fixedly connected with the barrel cover and is positioned above the barrel cover, the motor is arranged above the support, the output end of the motor penetrates through the support and is fixedly connected with the stirring assembly, one end of the air inlet pipe is fixedly connected with the reaction barrel and is positioned below the reaction barrel, the reaction barrel is communicated with the air inlet pipe, and the other end of the air inlet pipe is higher than the reaction barrel.
Through pouring into the nutrient solution raw materials extremely among the reaction barrel, use the bung lid closes the reaction barrel, and then starts the motor rotates to the drive the stirring subassembly rotates, will the inside nutrient solution raw materials of reaction barrel mix, through the intake pipe fills the carbon dioxide into the nutrient solution raw materials, because of the other end of intake pipe is higher than the reaction barrel, and then can utilize the linker principle, make the inside nutrient solution of reaction barrel can not flow, can be better through filling into of carbon dioxide simultaneously with the nutrient solution misce bene, the setting of above structure can make the carbon dioxide gas efficient contact with the nutrient solution to the pH valve of effectual regulation nutrient solution.
The stirring assembly comprises a stirring shaft and a plurality of blades, the stirring shaft is rotatably connected with the barrel cover and is positioned in the reaction barrel, and the blades are fixedly connected with the stirring shaft respectively and are positioned on the outer surface wall of the stirring shaft respectively.
The motor is started to rotate, so that the stirring shaft is driven to rotate, and the blades are used for stirring and mixing the nutrient solution.
Wherein, the support includes pillar and mounting panel, the quantity of pillar is many, many the pillar respectively with bung fixed connection, and be located respectively the top of bung, mounting panel and many pillar fixed connection, and be located many the top of pillar, the motor set up in the top of mounting panel.
Through the pillar is right the mounting panel supports, the mounting panel is right the motor is fixed, simultaneously the support of pillar is convenient for the output of motor with the (mixing) shaft is connected.
The production system of the one-step embryo culture solution further comprises a plurality of supporting columns and supporting plates, the number of the supporting columns is multiple, each supporting column is fixedly connected with the reaction barrel and is respectively located below the reaction barrel, the number of the supporting plates is multiple, and each supporting plate is respectively fixedly connected with the corresponding supporting column and is respectively located below the corresponding supporting column.
Through the support column is right the reaction barrel supports fixedly, simultaneously the setting of backup pad can increase the area of contact with ground, and then can make the reaction barrel is more stable.
Each supporting plate is provided with a plurality of mounting holes, and the mounting holes are uniformly distributed on the surface wall of the supporting plate.
Through the setting of mounting hole, can be right through the double-screw bolt the backup pad is fixed, and then makes the reaction barrel is more stable.
The production system of the one-step embryo culture solution further comprises a sealing plug, the sealing plug is in threaded connection with the reaction barrel, and the sealing plug penetrates through the reaction barrel.
Through the arrangement of the sealing plug, the reaction barrel can be sealed when carbon dioxide is not filled, foreign matters are prevented from entering the reaction barrel, and the reaction barrel can be decompressed when the sealing plug is taken down.
The invention also provides a preparation method of the one-step type embryo culture solution production system, which comprises the following steps:
adding raw materials of a nutrient solution into the reaction barrel;
slowly stirring the nutrient solution raw materials at a constant speed to uniformly mix the nutrient solution raw materials so as to obtain a solution I;
then, filling carbon dioxide gas into the solution I, and adjusting the pH value of the solution I;
then adding human serum albumin, insulin, transferrin, selenium, Fetuin-A and platelet derived growth factor PDGF into the solution I, stirring and mixing to obtain a solution II;
thus, the prepared solution II is balanced in a carbon dioxide incubator for 5 to 10 hours after being filtered and sterilized by a filter membrane of 0.2 um;
and finally, subpackaging the balanced solution II under the aseptic condition to complete the preparation of the embryo nutrient solution.
According to the one-step preparation method and production system of the embryo culture solution, nutrient solution raw materials are poured into the reaction barrel, the reaction barrel is covered by the barrel cover, the motor is started to rotate, the stirring assembly is driven to rotate, the nutrient solution raw materials in the reaction barrel are mixed, carbon dioxide is filled into the nutrient solution raw materials through the air inlet pipe, the other end of the air inlet pipe is higher than the reaction barrel, the nutrient solution in the reaction barrel cannot flow out due to the fact that the other end of the air inlet pipe is higher than the reaction barrel, meanwhile, the nutrient solution can be well and uniformly mixed due to the filling of the carbon dioxide, and due to the arrangement of the structure, the carbon dioxide can be in contact with the nutrient solution efficiently, and the pH value of the nutrient solution can be effectively adjusted.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic diagram of a one-step system for producing an embryo culture according to the present invention.
FIG. 2 is a top view of a one-step embryo culture fluid production system of the present invention.
Fig. 3 is a cross-sectional view of the a-a line structure of fig. 2 of the present invention.
Fig. 4 is an enlarged view of a portion of the structure of fig. 3B according to the present invention.
FIG. 5 is a flow chart of the steps of a one-step method of preparing an embryo culture of the present invention.
1-reaction barrel, 2-barrel cover, 3-bracket, 4-motor, 5-stirring component, 6-air inlet pipe, 7-stirring shaft, 8-blade, 9-pillar, 10-mounting plate, 11-supporting column, 12-supporting plate, 13-mounting hole, 14-sealing plug, 15-first connecting pipe, 16-first filter, 17-second filter, 18-screen plate, 19-filter core, 20-placing pipe, 21-second connecting pipe, 22-fixing column, 23-fixing block, 24-placing block and 25-reinforcing rod.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 4, the present invention provides a one-step embryo culture solution production system, which comprises a reaction barrel 1, a barrel cover 2, a bracket 3, a motor 4, a stirring component 5 and an air inlet pipe 6, wherein the barrel cover 2 is fixedly connected with the reaction barrel 1, the reaction barrel 1 is covered, the stirring component 5 is rotationally connected with the barrel cover 2 and is positioned in the reaction barrel 1, the bracket 3 is fixedly connected with the barrel cover 2, and is positioned above the barrel cover 2, the motor 4 is arranged above the bracket 3, the output end of the motor 4 penetrates through the bracket 3 and is fixedly connected with the stirring component 5, one end of the air inlet pipe 6 is fixedly connected with the reaction barrel 1, and is located the below of retort 1, and retort 1 with intake pipe 6 intercommunication, the other end of intake pipe 6 is higher than retort 1.
In this embodiment, through pour into the nutrient solution raw materials extremely among the reaction barrel 1, use 2 lids of bung close reaction barrel 1, and then start motor 4 rotates, thereby drives stirring subassembly 5 rotates, will 1 inside nutrient solution raw materials of reaction barrel mix, through intake pipe 6 fills carbon dioxide into the nutrient solution raw materials, because of the other end of intake pipe 6 is higher than reaction barrel 1, and then can utilize the linker principle, makes 1 inside nutrient solution of reaction barrel can not flow, simultaneously through filling into of carbon dioxide can be better with the nutrient solution misce bene, support 3 is right motor 4 supports fixedly, and the setting of above structure can make carbon dioxide gas efficient and nutrient solution contact to the pH valve of effectual regulation nutrient solution.
Further, the stirring assembly 5 comprises a stirring shaft 7 and a plurality of blades 8, the stirring shaft 7 is rotatably connected with the barrel cover 2 and is positioned inside the reaction barrel 1, the blades 8 are multiple in number, and the blades 8 are respectively fixedly connected with the stirring shaft 7 and are respectively positioned on the outer surface wall of the stirring shaft 7.
In this embodiment, the motor 4 is started to rotate, and then the stirring shaft 7 is driven to rotate, so that the blades 8 stir and mix the nutrient solution.
Further, support 3 includes pillar 9 and mounting panel 10, the quantity of pillar 9 is many, many pillar 9 respectively with bung 2 fixed connection, and be located respectively bung 2's top, mounting panel 10 and many pillar 9 fixed connection, and be located many pillar 9's top, motor 4 set up in mounting panel 10's top.
In this embodiment, the mounting plate 10 is supported by the support column 9, the mounting plate 10 fixes the motor 4, and the support column 9 facilitates the connection between the output end of the motor 4 and the stirring shaft 7.
Further, production system of one-step embryo culture liquid still includes support column 11 and backup pad 12, the quantity of support column 11 is many, every support column 11 respectively with reaction barrel 1 fixed connection, and be located respectively the below of reaction barrel 1, the quantity of backup pad 12 is a plurality of, every backup pad 12 respectively with correspond support column 11 fixed connection, and be located respectively the corresponding below of support column 11.
In this embodiment, the support column 11 is used to support and fix the reaction barrel 1, and the support plate 12 is arranged to increase the contact area with the ground, so that the reaction barrel 1 is more stable.
Further, each support plate 12 has a plurality of mounting holes 13, and the number of the mounting holes 13 is multiple, and the mounting holes 13 are uniformly distributed on the surface wall of the support plate 12.
In this embodiment, the mounting holes 13 may be formed to fix the support plate 12 by a stud, so that the reaction barrel 1 is more stable.
Further, the one-step embryo culture solution production system further comprises a sealing plug 14, wherein the sealing plug 14 is in threaded connection with the reaction barrel 1, and the sealing plug 14 penetrates through the reaction barrel 1.
In this embodiment, the reaction vessel 1 can be sealed to prevent the entry of foreign substances when the carbon dioxide is not introduced by providing the sealing plug 14, and the reaction vessel 1 can be depressurized by removing the sealing plug 14 when the carbon dioxide is introduced.
Further, the one-step embryo culture solution production system further comprises a first connecting pipe 15, a first filter 16 and a second filter 17, wherein the first connecting pipe 15 is communicated with the reaction barrel 1 and is positioned on the outer wall of the reaction barrel 1, the first filter 16 is communicated with the first connecting pipe 15 and is positioned at one end of the first connecting pipe 15, the second filter 17 is communicated with the air inlet pipe 6 and is positioned at one end of the air inlet pipe 6 far away from the reaction barrel 1, the structures of the first filter 16 and the second filter 17 are consistent, the first filter 16 comprises a screen plate 18, a filter element 19, a placing pipe 20 and a second connecting pipe 21, the placing pipe 20 is communicated with the first connecting pipe 15 and is positioned at one end of the first connecting pipe 15, the number of the screen plates 18 is two, the two screen plates 18 are respectively arranged inside the placing pipe 20, the filter element 19 is arranged between the two mesh plates 18, and the second connecting pipe 21 is communicated with the placing pipe 20 and is positioned at one end of the placing pipe 20.
In this embodiment, carbon dioxide is introduced from the first filter 16, so that the air pressure in the reaction barrel 1 is increased, and then the nutrient solution is discharged from the air inlet pipe 6, the filter element 19 can prevent foreign matters from entering, the filter element 19 can be limited by the mesh plate 18, the mesh plate 18 and the filter element 19 are placed by the placing pipe 20, the second connecting pipe 21 is convenient for connecting an air pipe, and meanwhile, the second filter 17 can filter residues in the nutrient solution, and then the quality of the nutrient solution is improved.
Further, the production system of the one-step embryo culture solution further comprises two fixing columns 22, two fixing blocks 23 and two placing blocks 24, the placing blocks 24 are arranged on the outer wall of the air inlet pipe 6, the two fixing columns 22 are respectively and fixedly connected with the placing blocks 24 and respectively located below the placing blocks 24, the two fixing blocks 23 are respectively and fixedly connected with the corresponding fixing columns 22 and respectively located below the corresponding fixing columns 22, and the two fixing blocks 23 are respectively and fixedly connected with the corresponding fixing columns 22 and respectively located below the corresponding fixing columns 22.
In this embodiment, the fixing column 22 and the placing block 24 support and fix the air inlet pipe 6, so as to prevent the air inlet pipe 6 from collapsing, and the fixing block 23 facilitates installation and fixation of the fixing column 22, so that the fixing column 22 is more stable.
Further, the production system of the one-step embryo culture solution further comprises a plurality of reinforcing rods 25, the number of the reinforcing rods 25 is multiple, and two ends of each reinforcing rod 25 are respectively fixedly connected with the two fixing columns 22 and are respectively located between the two fixing columns 22.
In the present embodiment, the reinforcing bar 25 is provided to enhance the stability of the fixing post 22, so that the fixing post 22 is more stable.
Referring to fig. 5, the present invention further provides a method for preparing a one-step embryo culture fluid production system, comprising the following steps:
s1: adding raw materials of nutrient solution into the reaction barrel 1;
s2: slowly stirring the nutrient solution raw materials at a constant speed to uniformly mix the nutrient solution raw materials so as to obtain a solution I;
s3: then, filling carbon dioxide gas into the solution I, and adjusting the pH value of the solution I;
s4: then adding human serum albumin, insulin, transferrin, selenium, Fetuin-A and platelet derived growth factor PDGF into the solution I, stirring and mixing to obtain a solution II;
s5: thus, the prepared solution II is balanced in a carbon dioxide incubator for 5 to 10 hours after being filtered and sterilized by a filter membrane of 0.2 um;
s6: and finally, subpackaging the balanced solution II under the aseptic condition to complete the preparation of the embryo nutrient solution.
The embryo nutrient solution is prepared by adding raw materials of the nutrient solution into the reaction barrel 1, stirring and mixing the raw materials, filling carbon dioxide into the raw materials to adjust the pH value of the nutrient solution, adding human serum albumin, insulin, transferrin, selenium, Fetuin-A and platelet-derived growth factor PDGF into the nutrient solution, stirring and mixing the raw materials, filtering and sterilizing the nutrient solution by using a 0.2um filter membrane, balancing the nutrient solution in a carbon dioxide incubator for 5 to 10 hours, and finally subpackaging the nutrient solution under the aseptic condition.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (7)

1. A one-step embryo culture solution production system is characterized in that,
including reaction barrel, bung, support, motor, stirring subassembly and intake pipe, the bung with reaction barrel fixed connection to the lid closes reaction barrel, the stirring subassembly with the bung rotates to be connected, and is located reaction barrel's inside, the support with bung fixed connection, and be located the top of bung, the motor set up in the top of support, just the output of motor runs through the support and with stirring subassembly fixed connection, the one end of intake pipe with reaction barrel fixed connection, and be located reaction barrel's below, just reaction barrel with the intake pipe intercommunication, the other end of intake pipe is higher than reaction barrel.
2. The one-step embryo culture fluid production system of claim 1,
the stirring assembly comprises a stirring shaft and a plurality of blades, the stirring shaft is rotatably connected with the barrel cover and is positioned in the reaction barrel, and the blades are fixedly connected with the stirring shaft respectively and are positioned on the outer surface wall of the stirring shaft respectively.
3. The one-step embryo culture fluid production system of claim 2,
the support includes pillar and mounting panel, the quantity of pillar is many, many the pillar respectively with bung fixed connection, and be located respectively the top of bung, mounting panel and many pillar fixed connection, and be located many the top of pillar, the motor set up in the top of mounting panel.
4. The one-step embryo culture fluid production system of claim 3,
the production system of one-step embryo culture liquid still includes support column and backup pad, the quantity of support column is many, every the support column respectively with retort fixed connection to be located respectively the below of retort, the quantity of backup pad is a plurality of, every the backup pad respectively with correspond support column fixed connection, and be located respectively the correspondence the below of support column.
5. The one-step embryo culture fluid production system of claim 4,
each backup pad has the mounting hole, the quantity of mounting hole is a plurality of, and is a plurality of mounting hole evenly distributed in the table wall of backup pad.
6. The one-step embryo culture fluid production system of claim 5,
the production system of the one-step embryo culture solution further comprises a sealing plug, the sealing plug is in threaded connection with the reaction barrel, and the sealing plug penetrates through the reaction barrel.
7. The method of claim 6, comprising the steps of:
adding raw materials of a nutrient solution into the reaction barrel;
slowly stirring the nutrient solution raw materials at a constant speed to uniformly mix the nutrient solution raw materials so as to obtain a solution I;
then, filling carbon dioxide gas into the solution I, and adjusting the pH value of the solution I;
then adding human serum albumin, insulin, transferrin, selenium, Fetuin-A and platelet derived growth factor PDGF into the solution I, stirring and mixing to obtain a solution II;
thus, the prepared solution II is balanced in a carbon dioxide incubator for 5 to 10 hours after being filtered and sterilized by a filter membrane of 0.2 um;
and finally, subpackaging the balanced solution II under the aseptic condition to complete the preparation of the embryo nutrient solution.
CN202111326231.8A 2021-11-10 2021-11-10 One-step preparation method and production system of embryo culture solution Pending CN113897290A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
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