CN113893388A - Modular tissue engineering bone-ligament-bone graft and preparation method thereof - Google Patents
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Abstract
The invention relates to a preparation method of a modular tissue engineering bone-ligament-bone graft, which comprises the following steps: doping beta-tricalcium phosphate into two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, so as to prepare a collagen-tricalcium phosphate membrane; coating platelet-rich plasma-fibrin gel on collagen-tricalcium phosphate membrane to obtain bone-ligament-periosteum; inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain tissue engineering bone-ligament-periosteum; overlapping and compounding a plurality of tissue engineering bones-ligaments-periosteum to obtain the tissue engineering bone-ligament-bone graft. The invention constructs an organoid structure simulating bone-ligament-bone, explores a molecular mechanism of ligament injury and tendon-bone interface healing in vitro, and further utilizes the organoids to prepare a tissue engineering bone-ligament-bone graft for repairing and regenerating ligament injury and tendon-bone interface tissues.
Description
Technical Field
The invention relates to the field of medical treatment, in particular to a modular tissue engineering bone-ligament-bone graft and a preparation method thereof.
Background
Ligament (e.g., cruciate ligament, collateral ligament, etc.) soft tissue injuries are very common sports injuries, with 50% of injuries in juvenile sports being ligament sprains. At the same time, the incidence of Anterior Cruciate Ligament (ACL) injury increased by 1.3% over the past decade. Except for the sports population, the probability of tendon ligament injury of a diabetic patient is ten times that of a non-diabetic patient. With age, 80% of the elderly experience ligament rupture and tendon injury. Although tendon ligament injuries are very common and have a tremendous impact on individuals, families, and economics, advances in prevention and post-injury repair have been limited.
At present, the prevention and treatment of ligament injury mainly comprises brake fixation, medication and operation repair. The brake fixation is to fix the affected limb through plaster, so as to avoid the ligament tissue from being damaged again by the movement; the drug therapy is mainly to promote the repair of ligament tissues or relieve pain through the pharmaceutical action; surgical repair is the surgical restoration of the physical structure of the lesion to promote its healing. However, the current treatment means often causes the scar of the ligament tissue to heal, even not heal, mainly because the current mechanism for healing and regenerating the damaged ligament is not deeply understood, and the high-efficiency and high-quality tendon graft or tendon prosthesis for repairing the damaged ligament is lacking at present.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a modular tissue engineering bone-ligament-bone graft and a preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
the first aspect of the invention provides a preparation method of a modular tissue engineering bone-ligament-bone graft, which comprises the following steps:
s1, doping beta-tricalcium phosphate into the two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, and thus the collagen-tricalcium phosphate membrane is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding a plurality of the tissue engineering bones, ligaments and periosteum to obtain the tissue engineering bone, ligament and bone graft.
Preferably, the oriented collagen fiber membrane is prepared from collagen by an electrospinning technique.
Preferably, the oriented collagen fiber membrane has a thickness of 0.01 to 1 mm.
Further, the thickness of the oriented collagen fiber membrane is 0.02-0.2 mm.
Preferably, the beta-tricalcium phosphate is doped to a thickness of 0.01 to 1 mm.
Furthermore, the thickness of the doped beta-tricalcium phosphate is 0.02-0.2 mm.
Preferably, the doped beta-tricalcium phosphate has a density of 0-3g/cm3。
Further, the density of the doped beta-tricalcium phosphate is 0.01-1g/cm3。
Preferably, the platelet rich plasma-fibrin gel is prepared by mixing platelet rich plasma and fibrin in a ratio of 1:3-3: 1.
Preferably, the platelet rich plasma is prepared from peripheral blood by anticoagulation and in vitro centrifugation (without thrombin addition) in sequence.
Preferably, the in vitro culture takes 80% -90% of the consumption of the platelet rich plasma-fibrin as a culture end point.
Preferably, the number of the tissue engineering bone-ligament-periosteum overlap compound is 6-60 pieces.
The second aspect of the present invention is to provide a modular tissue engineered bone-ligament-bone graft prepared by the preparation method as described above.
By adopting the technical scheme, compared with the prior art, the invention has the following technical effects:
the invention constructs an organoid structure simulating bone-ligament-bone, explores a molecular mechanism of ligament injury and tendon-bone interface healing in vitro, further utilizes the organoids to prepare a tissue engineering bone-ligament-bone graft, repairs and regenerates ligament injury and tendon-bone interface tissues, and regenerates a bone-cartilage-tendon interface structure.
Drawings
FIG. 1 is a flow chart of the preparation of the tissue engineered bone-ligament-periosteum of the present invention;
FIG. 2 is a schematic structural diagram of the tissue engineered bone-ligament-bone graft of the present invention;
FIG. 3 is a graph showing the results of detection examples 1 to 3 according to the present invention;
FIG. 4 is a graph showing HE staining results of detection example 4 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
Examples
As shown in fig. 1-2, the present embodiment provides a modular tissue engineered bone-ligament-bone graft, which is prepared by the following steps:
s1, taking 50mL of peripheral blood, performing anticoagulation and then performing in-vitro centrifugation for 15-20min (without adding thrombin), and preparing platelet-rich plasma; mixing the platelet-rich plasma and fibrin according to the proportion of 1:1 to prepare platelet-rich plasma-fibrin gel; preparing collagen into an oriented collagen fiber membrane by adopting an electrostatic spinning technology, wherein the thickness of the oriented collagen fiber membrane is 0.02-0.2 mm; doping the two ends of the oriented collagen fiber membrane by gradient chemical precipitation method to obtain a mixture with a thickness of 0.02-0.2mm and a density of 0.01-1g/cm3The density of the doped beta-tricalcium phosphate is gradually reduced from the end part to the middle part, and a collagen-tricalcium phosphate film is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, culturing in vitro, and taking 80% -90% of the platelet-rich plasma-fibrin consumption as a culture end point to prepare the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding 6-60 pieces of the tissue engineering bone-ligament-periosteum to obtain the tissue engineering bone-ligament-bone graft.
Detection example 1
In order to verify that the collagen membrane has the function of inducing the differentiation of the tendon of the stem cell, mesenchymal stem cells are inoculated on the oriented collagen fiber membrane (COL group) and the collagen-tricalcium phosphate membrane (COL-TCP group), a blank pore plate is used as a control group (namely CON group), a common culture medium is adopted for culturing for 7d, and the expression of tendon-related genes Scx and Mkx in the stem cell is detected, as shown in figures 3a and 3b, the expression of the tendon-related genes in the COL group stem cell is obviously increased compared with the control group and the COL-TCP group, which indicates that the oriented collagen fiber membrane has a better function of inducing the differentiation of the tendon of the stem cell.
Detection example 2
In order to verify that the collagen-tricalcium phosphate membrane has the function of inducing the osteogenic differentiation of the stem cells, mesenchymal stem cells are inoculated on the oriented collagen fiber membrane (COL group) and the collagen-tricalcium phosphate membrane (COL-TCP group), a blank pore plate is used as a control group (namely CON group), and the common culture medium is adopted for culture for 7d, so that the expression of the osteogenic related genes ALP and OCN in the stem cells is detected, as shown in figures 3c and 3d, the expression of the osteogenic related genes in the COL-TCP group stem cells is obviously increased compared with the control group and the COL group, and the collagen-tricalcium phosphate membrane has a better function of inducing the osteogenic differentiation of the stem cells.
Detection example 3
In order to verify that the collagen-tricalcium phosphate density gradient structure has the function of inducing the stem cell to be chondrogenic differentiated, the mesenchymal stem cells are inoculated on collagen fiber membranes-tricalcium phosphate membranes (the densities are 1, 2, 3, 4, 5 and 6 respectively) containing different TCP densities, a blank orifice plate is used as a control group (namely a CON group), a common culture medium is adopted for culture for 7d, and the expression of the cartilage related gene Sox9 in the stem cells is detected, as shown in figure 3e, the collagen fiber membranes-tricalcium phosphate membranes with different TCP densities have different functions of inducing the stem cells to be chondrogenic differentiated, wherein the cartilage related gene expression in the stem cells in the group with the density of 3 is obviously increased compared with the control group and other groups, and the regulation of the TCP density can effectively regulate the chondrogenic function of the collagen fiber-tricalcium phosphate membranes.
Detection example 4
In animal experiments, a Xinzealand rabbit knee joint anterior fork reconstruction model is adopted, a control group is a collagen ligament, an experimental group is a bone-collagen-bone ligament, materials are taken 3 months after operation, HE staining is respectively carried out on a ligament part in a joint cavity and an intra-osseous tendon and bone interface part, and the result is shown in figure 4.
In conclusion, the invention constructs an organoid structure simulating bone-ligament-bone in vitro, which comprises bone tissue phases at two ends and a middle ligament tissue phase, wherein the bone tissue phase induces mesenchymal stem cells to differentiate into osteoblasts, the ligament tissue phase induces the mesenchymal stem cells to differentiate into tenoblasts, and the bone-ligament junction gradient gradually induces the mesenchymal stem cells to differentiate into chondroblasts; by virtue of this bone-ligament-bone organoid structure, it is possible to study the specific molecular mechanisms of ligament tissue damage and tendon-bone interface damage and to explore effective drugs or methods (including but not limited to material structures) that promote ligament healing and tendon-bone interface regeneration;
the invention is based on the bone-ligament-bone organoid structure, and is inoculated with seed cells (such as mesenchymal stem cells, adipose-derived stem cells and the like), so that a bionic tissue engineering bone-ligament-periosteum can be prepared, and then a tissue engineering bone-ligament-bone graft is prepared, so that the repair and regeneration of ligament injury are realized, and a bone-cartilage-tendon interface structure is regenerated.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.
Claims (9)
1. A preparation method of a modular tissue engineering bone-ligament-bone graft is characterized by comprising the following steps:
s1, doping beta-tricalcium phosphate into the two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, and thus the collagen-tricalcium phosphate membrane is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding a plurality of the tissue engineering bones, ligaments and periosteum to obtain the tissue engineering bone, ligament and bone graft.
2. The method of claim 1, wherein the oriented collagen fiber membrane is prepared from collagen by an electrospinning technique.
3. The method for preparing the collagen fiber membrane according to claim 1, wherein the oriented collagen fiber membrane has a thickness of 0.01 to 1 mm.
4. The method for preparing the collagen fiber membrane according to claim 3, wherein the oriented collagen fiber membrane has a thickness of 0.02 to 0.2 mm.
5. The method of claim 1, wherein the β -tricalcium phosphate is doped to a thickness of 0.01 to 1mm and the β -tricalcium phosphate is doped to a density of 0 to 3g/cm3。
6. The method of claim 5, wherein the β -tricalcium phosphate is doped to a thickness of 0.02 to 0.2mm and the β -tricalcium phosphate is doped to a density of 0.01 to 1g/cm3。
7. The method according to claim 1, wherein the platelet-rich plasma-fibrin gel is prepared by mixing platelet-rich plasma and fibrin at a ratio of 1:3 to 3: 1.
8. The method of claim 1, wherein the in vitro culture has 80% -90% of the platelet rich plasma-fibrin consumed as a culture endpoint.
9. A modular tissue engineered bone-ligament-bone graft made by the method of manufacture of any one of claims 1-8.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114949353A (en) * | 2022-01-24 | 2022-08-30 | 东华大学 | Biological function differentiated adaptation musculoskeletal system prosthesis and preparation method thereof |
CN115068687A (en) * | 2022-07-08 | 2022-09-20 | 重庆科技学院 | Gradient nano/microfiber scaffold and preparation method and application thereof |
Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2824875Y (en) * | 2005-04-14 | 2006-10-11 | 南方医院 | An injection type tissue engineering bone engraft |
CN1846790A (en) * | 2005-04-14 | 2006-10-18 | 南方医院 | Injected tissue engineering bone and its constrction and application |
US20080112998A1 (en) * | 2006-11-14 | 2008-05-15 | Hongjun Wang | Innovative bottom-up cell assembly approach to three-dimensional tissue formation using nano-or micro-fibers |
US20090062870A1 (en) * | 2007-08-27 | 2009-03-05 | Giuseppe Milano | Method of arthroscopic osteochondral resurfacing using prp strengthened with fibrin glue |
CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
CA2826771A1 (en) * | 2012-09-13 | 2014-03-13 | The Uab Research Foundation | Biomimetic tissue graft for ligament replacement |
WO2015162559A1 (en) * | 2014-04-21 | 2015-10-29 | Ineb - Instituto Nacional De Engenharia Biomédica | Mesh composition for repairing or the regeneration of tissues and methods thereof |
CN107349475A (en) * | 2017-07-14 | 2017-11-17 | 中国人民解放军第四军医大学 | Artificial organ engineering skin that nano fibrous membrane is layering with stem cell and preparation method thereof |
CN108653328A (en) * | 2018-03-26 | 2018-10-16 | 杭州市萧山区中医院 | Application of the mesenchyma stem cell combined platelet rich plasma in preparing the drug for promoting the healing of rotator cuff injury tendon bone complex |
CN110693629A (en) * | 2019-11-19 | 2020-01-17 | 上海市第六人民医院 | Sectional and gradient functional tendon sleeve implantation instrument and preparation method thereof |
CN110898253A (en) * | 2019-12-17 | 2020-03-24 | 浙江大学医学院附属第一医院 | Method for preparing intrafiber biomimetic mineralized collagen membrane capable of promoting cell osteogenic differentiation |
CN111346262A (en) * | 2020-03-17 | 2020-06-30 | 四川大学 | Injectable calcium-phosphorus ceramic for promoting healing of tendon and bone and preparation method and application thereof |
CN112999424A (en) * | 2021-02-08 | 2021-06-22 | 四川大学 | Composite electrospun membrane for promoting healing of aponeurosis as well as preparation method and application thereof |
CN113368308A (en) * | 2021-06-07 | 2021-09-10 | 北京市创伤骨科研究所 | Bionic sandwich-structured artificial periosteum and preparation method thereof |
-
2021
- 2021-09-23 CN CN202111117609.3A patent/CN113893388A/en active Pending
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2824875Y (en) * | 2005-04-14 | 2006-10-11 | 南方医院 | An injection type tissue engineering bone engraft |
CN1846790A (en) * | 2005-04-14 | 2006-10-18 | 南方医院 | Injected tissue engineering bone and its constrction and application |
US20080112998A1 (en) * | 2006-11-14 | 2008-05-15 | Hongjun Wang | Innovative bottom-up cell assembly approach to three-dimensional tissue formation using nano-or micro-fibers |
US20090062870A1 (en) * | 2007-08-27 | 2009-03-05 | Giuseppe Milano | Method of arthroscopic osteochondral resurfacing using prp strengthened with fibrin glue |
CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
CA2826771A1 (en) * | 2012-09-13 | 2014-03-13 | The Uab Research Foundation | Biomimetic tissue graft for ligament replacement |
WO2015162559A1 (en) * | 2014-04-21 | 2015-10-29 | Ineb - Instituto Nacional De Engenharia Biomédica | Mesh composition for repairing or the regeneration of tissues and methods thereof |
CN107349475A (en) * | 2017-07-14 | 2017-11-17 | 中国人民解放军第四军医大学 | Artificial organ engineering skin that nano fibrous membrane is layering with stem cell and preparation method thereof |
CN108653328A (en) * | 2018-03-26 | 2018-10-16 | 杭州市萧山区中医院 | Application of the mesenchyma stem cell combined platelet rich plasma in preparing the drug for promoting the healing of rotator cuff injury tendon bone complex |
CN110693629A (en) * | 2019-11-19 | 2020-01-17 | 上海市第六人民医院 | Sectional and gradient functional tendon sleeve implantation instrument and preparation method thereof |
CN110898253A (en) * | 2019-12-17 | 2020-03-24 | 浙江大学医学院附属第一医院 | Method for preparing intrafiber biomimetic mineralized collagen membrane capable of promoting cell osteogenic differentiation |
CN111346262A (en) * | 2020-03-17 | 2020-06-30 | 四川大学 | Injectable calcium-phosphorus ceramic for promoting healing of tendon and bone and preparation method and application thereof |
CN112999424A (en) * | 2021-02-08 | 2021-06-22 | 四川大学 | Composite electrospun membrane for promoting healing of aponeurosis as well as preparation method and application thereof |
CN113368308A (en) * | 2021-06-07 | 2021-09-10 | 北京市创伤骨科研究所 | Bionic sandwich-structured artificial periosteum and preparation method thereof |
Non-Patent Citations (1)
Title |
---|
王迎军: "新型材料科学与技术 无机材料卷 下", 广州:华南理工大学出版社, pages: 1335 * |
Cited By (3)
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CN114949353A (en) * | 2022-01-24 | 2022-08-30 | 东华大学 | Biological function differentiated adaptation musculoskeletal system prosthesis and preparation method thereof |
CN115068687A (en) * | 2022-07-08 | 2022-09-20 | 重庆科技学院 | Gradient nano/microfiber scaffold and preparation method and application thereof |
CN115068687B (en) * | 2022-07-08 | 2023-12-12 | 重庆科技学院 | Gradient nano/micro fiber support and preparation method and application thereof |
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