CN113893388A - Modular tissue engineering bone-ligament-bone graft and preparation method thereof - Google Patents

Modular tissue engineering bone-ligament-bone graft and preparation method thereof Download PDF

Info

Publication number
CN113893388A
CN113893388A CN202111117609.3A CN202111117609A CN113893388A CN 113893388 A CN113893388 A CN 113893388A CN 202111117609 A CN202111117609 A CN 202111117609A CN 113893388 A CN113893388 A CN 113893388A
Authority
CN
China
Prior art keywords
bone
ligament
tricalcium phosphate
tissue engineering
periosteum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111117609.3A
Other languages
Chinese (zh)
Inventor
赵金忠
李亚民
崔文国
蒋佳
赵松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Sixth Peoples Hospital
Original Assignee
Shanghai Sixth Peoples Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Sixth Peoples Hospital filed Critical Shanghai Sixth Peoples Hospital
Priority to CN202111117609.3A priority Critical patent/CN113893388A/en
Publication of CN113893388A publication Critical patent/CN113893388A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3616—Blood, e.g. platelet-rich plasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Public Health (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Inorganic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Materials Engineering (AREA)
  • Composite Materials (AREA)
  • Vascular Medicine (AREA)
  • Cell Biology (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention relates to a preparation method of a modular tissue engineering bone-ligament-bone graft, which comprises the following steps: doping beta-tricalcium phosphate into two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, so as to prepare a collagen-tricalcium phosphate membrane; coating platelet-rich plasma-fibrin gel on collagen-tricalcium phosphate membrane to obtain bone-ligament-periosteum; inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain tissue engineering bone-ligament-periosteum; overlapping and compounding a plurality of tissue engineering bones-ligaments-periosteum to obtain the tissue engineering bone-ligament-bone graft. The invention constructs an organoid structure simulating bone-ligament-bone, explores a molecular mechanism of ligament injury and tendon-bone interface healing in vitro, and further utilizes the organoids to prepare a tissue engineering bone-ligament-bone graft for repairing and regenerating ligament injury and tendon-bone interface tissues.

Description

Modular tissue engineering bone-ligament-bone graft and preparation method thereof
Technical Field
The invention relates to the field of medical treatment, in particular to a modular tissue engineering bone-ligament-bone graft and a preparation method thereof.
Background
Ligament (e.g., cruciate ligament, collateral ligament, etc.) soft tissue injuries are very common sports injuries, with 50% of injuries in juvenile sports being ligament sprains. At the same time, the incidence of Anterior Cruciate Ligament (ACL) injury increased by 1.3% over the past decade. Except for the sports population, the probability of tendon ligament injury of a diabetic patient is ten times that of a non-diabetic patient. With age, 80% of the elderly experience ligament rupture and tendon injury. Although tendon ligament injuries are very common and have a tremendous impact on individuals, families, and economics, advances in prevention and post-injury repair have been limited.
At present, the prevention and treatment of ligament injury mainly comprises brake fixation, medication and operation repair. The brake fixation is to fix the affected limb through plaster, so as to avoid the ligament tissue from being damaged again by the movement; the drug therapy is mainly to promote the repair of ligament tissues or relieve pain through the pharmaceutical action; surgical repair is the surgical restoration of the physical structure of the lesion to promote its healing. However, the current treatment means often causes the scar of the ligament tissue to heal, even not heal, mainly because the current mechanism for healing and regenerating the damaged ligament is not deeply understood, and the high-efficiency and high-quality tendon graft or tendon prosthesis for repairing the damaged ligament is lacking at present.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a modular tissue engineering bone-ligament-bone graft and a preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
the first aspect of the invention provides a preparation method of a modular tissue engineering bone-ligament-bone graft, which comprises the following steps:
s1, doping beta-tricalcium phosphate into the two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, and thus the collagen-tricalcium phosphate membrane is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding a plurality of the tissue engineering bones, ligaments and periosteum to obtain the tissue engineering bone, ligament and bone graft.
Preferably, the oriented collagen fiber membrane is prepared from collagen by an electrospinning technique.
Preferably, the oriented collagen fiber membrane has a thickness of 0.01 to 1 mm.
Further, the thickness of the oriented collagen fiber membrane is 0.02-0.2 mm.
Preferably, the beta-tricalcium phosphate is doped to a thickness of 0.01 to 1 mm.
Furthermore, the thickness of the doped beta-tricalcium phosphate is 0.02-0.2 mm.
Preferably, the doped beta-tricalcium phosphate has a density of 0-3g/cm3。
Further, the density of the doped beta-tricalcium phosphate is 0.01-1g/cm3。
Preferably, the platelet rich plasma-fibrin gel is prepared by mixing platelet rich plasma and fibrin in a ratio of 1:3-3: 1.
Preferably, the platelet rich plasma is prepared from peripheral blood by anticoagulation and in vitro centrifugation (without thrombin addition) in sequence.
Preferably, the in vitro culture takes 80% -90% of the consumption of the platelet rich plasma-fibrin as a culture end point.
Preferably, the number of the tissue engineering bone-ligament-periosteum overlap compound is 6-60 pieces.
The second aspect of the present invention is to provide a modular tissue engineered bone-ligament-bone graft prepared by the preparation method as described above.
By adopting the technical scheme, compared with the prior art, the invention has the following technical effects:
the invention constructs an organoid structure simulating bone-ligament-bone, explores a molecular mechanism of ligament injury and tendon-bone interface healing in vitro, further utilizes the organoids to prepare a tissue engineering bone-ligament-bone graft, repairs and regenerates ligament injury and tendon-bone interface tissues, and regenerates a bone-cartilage-tendon interface structure.
Drawings
FIG. 1 is a flow chart of the preparation of the tissue engineered bone-ligament-periosteum of the present invention;
FIG. 2 is a schematic structural diagram of the tissue engineered bone-ligament-bone graft of the present invention;
FIG. 3 is a graph showing the results of detection examples 1 to 3 according to the present invention;
FIG. 4 is a graph showing HE staining results of detection example 4 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
Examples
As shown in fig. 1-2, the present embodiment provides a modular tissue engineered bone-ligament-bone graft, which is prepared by the following steps:
s1, taking 50mL of peripheral blood, performing anticoagulation and then performing in-vitro centrifugation for 15-20min (without adding thrombin), and preparing platelet-rich plasma; mixing the platelet-rich plasma and fibrin according to the proportion of 1:1 to prepare platelet-rich plasma-fibrin gel; preparing collagen into an oriented collagen fiber membrane by adopting an electrostatic spinning technology, wherein the thickness of the oriented collagen fiber membrane is 0.02-0.2 mm; doping the two ends of the oriented collagen fiber membrane by gradient chemical precipitation method to obtain a mixture with a thickness of 0.02-0.2mm and a density of 0.01-1g/cm3The density of the doped beta-tricalcium phosphate is gradually reduced from the end part to the middle part, and a collagen-tricalcium phosphate film is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, culturing in vitro, and taking 80% -90% of the platelet-rich plasma-fibrin consumption as a culture end point to prepare the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding 6-60 pieces of the tissue engineering bone-ligament-periosteum to obtain the tissue engineering bone-ligament-bone graft.
Detection example 1
In order to verify that the collagen membrane has the function of inducing the differentiation of the tendon of the stem cell, mesenchymal stem cells are inoculated on the oriented collagen fiber membrane (COL group) and the collagen-tricalcium phosphate membrane (COL-TCP group), a blank pore plate is used as a control group (namely CON group), a common culture medium is adopted for culturing for 7d, and the expression of tendon-related genes Scx and Mkx in the stem cell is detected, as shown in figures 3a and 3b, the expression of the tendon-related genes in the COL group stem cell is obviously increased compared with the control group and the COL-TCP group, which indicates that the oriented collagen fiber membrane has a better function of inducing the differentiation of the tendon of the stem cell.
Detection example 2
In order to verify that the collagen-tricalcium phosphate membrane has the function of inducing the osteogenic differentiation of the stem cells, mesenchymal stem cells are inoculated on the oriented collagen fiber membrane (COL group) and the collagen-tricalcium phosphate membrane (COL-TCP group), a blank pore plate is used as a control group (namely CON group), and the common culture medium is adopted for culture for 7d, so that the expression of the osteogenic related genes ALP and OCN in the stem cells is detected, as shown in figures 3c and 3d, the expression of the osteogenic related genes in the COL-TCP group stem cells is obviously increased compared with the control group and the COL group, and the collagen-tricalcium phosphate membrane has a better function of inducing the osteogenic differentiation of the stem cells.
Detection example 3
In order to verify that the collagen-tricalcium phosphate density gradient structure has the function of inducing the stem cell to be chondrogenic differentiated, the mesenchymal stem cells are inoculated on collagen fiber membranes-tricalcium phosphate membranes (the densities are 1, 2, 3, 4, 5 and 6 respectively) containing different TCP densities, a blank orifice plate is used as a control group (namely a CON group), a common culture medium is adopted for culture for 7d, and the expression of the cartilage related gene Sox9 in the stem cells is detected, as shown in figure 3e, the collagen fiber membranes-tricalcium phosphate membranes with different TCP densities have different functions of inducing the stem cells to be chondrogenic differentiated, wherein the cartilage related gene expression in the stem cells in the group with the density of 3 is obviously increased compared with the control group and other groups, and the regulation of the TCP density can effectively regulate the chondrogenic function of the collagen fiber-tricalcium phosphate membranes.
Detection example 4
In animal experiments, a Xinzealand rabbit knee joint anterior fork reconstruction model is adopted, a control group is a collagen ligament, an experimental group is a bone-collagen-bone ligament, materials are taken 3 months after operation, HE staining is respectively carried out on a ligament part in a joint cavity and an intra-osseous tendon and bone interface part, and the result is shown in figure 4.
In conclusion, the invention constructs an organoid structure simulating bone-ligament-bone in vitro, which comprises bone tissue phases at two ends and a middle ligament tissue phase, wherein the bone tissue phase induces mesenchymal stem cells to differentiate into osteoblasts, the ligament tissue phase induces the mesenchymal stem cells to differentiate into tenoblasts, and the bone-ligament junction gradient gradually induces the mesenchymal stem cells to differentiate into chondroblasts; by virtue of this bone-ligament-bone organoid structure, it is possible to study the specific molecular mechanisms of ligament tissue damage and tendon-bone interface damage and to explore effective drugs or methods (including but not limited to material structures) that promote ligament healing and tendon-bone interface regeneration;
the invention is based on the bone-ligament-bone organoid structure, and is inoculated with seed cells (such as mesenchymal stem cells, adipose-derived stem cells and the like), so that a bionic tissue engineering bone-ligament-periosteum can be prepared, and then a tissue engineering bone-ligament-bone graft is prepared, so that the repair and regeneration of ligament injury are realized, and a bone-cartilage-tendon interface structure is regenerated.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.

Claims (9)

1. A preparation method of a modular tissue engineering bone-ligament-bone graft is characterized by comprising the following steps:
s1, doping beta-tricalcium phosphate into the two ends of the oriented collagen fiber membrane by adopting a gradient chemical precipitation method, wherein the density of the doped beta-tricalcium phosphate is gradually reduced from the end parts to the middle part, and thus the collagen-tricalcium phosphate membrane is prepared; coating platelet-rich plasma-fibrin gel on the collagen-tricalcium phosphate membrane to prepare bone-ligament-periosteum;
s2, inoculating seed cells on the bone-ligament-periosteum, and culturing in vitro to obtain the tissue engineering bone-ligament-periosteum;
s3, overlapping and compounding a plurality of the tissue engineering bones, ligaments and periosteum to obtain the tissue engineering bone, ligament and bone graft.
2. The method of claim 1, wherein the oriented collagen fiber membrane is prepared from collagen by an electrospinning technique.
3. The method for preparing the collagen fiber membrane according to claim 1, wherein the oriented collagen fiber membrane has a thickness of 0.01 to 1 mm.
4. The method for preparing the collagen fiber membrane according to claim 3, wherein the oriented collagen fiber membrane has a thickness of 0.02 to 0.2 mm.
5. The method of claim 1, wherein the β -tricalcium phosphate is doped to a thickness of 0.01 to 1mm and the β -tricalcium phosphate is doped to a density of 0 to 3g/cm3。
6. The method of claim 5, wherein the β -tricalcium phosphate is doped to a thickness of 0.02 to 0.2mm and the β -tricalcium phosphate is doped to a density of 0.01 to 1g/cm3。
7. The method according to claim 1, wherein the platelet-rich plasma-fibrin gel is prepared by mixing platelet-rich plasma and fibrin at a ratio of 1:3 to 3: 1.
8. The method of claim 1, wherein the in vitro culture has 80% -90% of the platelet rich plasma-fibrin consumed as a culture endpoint.
9. A modular tissue engineered bone-ligament-bone graft made by the method of manufacture of any one of claims 1-8.
CN202111117609.3A 2021-09-23 2021-09-23 Modular tissue engineering bone-ligament-bone graft and preparation method thereof Pending CN113893388A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111117609.3A CN113893388A (en) 2021-09-23 2021-09-23 Modular tissue engineering bone-ligament-bone graft and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111117609.3A CN113893388A (en) 2021-09-23 2021-09-23 Modular tissue engineering bone-ligament-bone graft and preparation method thereof

Publications (1)

Publication Number Publication Date
CN113893388A true CN113893388A (en) 2022-01-07

Family

ID=79029128

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111117609.3A Pending CN113893388A (en) 2021-09-23 2021-09-23 Modular tissue engineering bone-ligament-bone graft and preparation method thereof

Country Status (1)

Country Link
CN (1) CN113893388A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949353A (en) * 2022-01-24 2022-08-30 东华大学 Biological function differentiated adaptation musculoskeletal system prosthesis and preparation method thereof
CN115068687A (en) * 2022-07-08 2022-09-20 重庆科技学院 Gradient nano/microfiber scaffold and preparation method and application thereof

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2824875Y (en) * 2005-04-14 2006-10-11 南方医院 An injection type tissue engineering bone engraft
CN1846790A (en) * 2005-04-14 2006-10-18 南方医院 Injected tissue engineering bone and its constrction and application
US20080112998A1 (en) * 2006-11-14 2008-05-15 Hongjun Wang Innovative bottom-up cell assembly approach to three-dimensional tissue formation using nano-or micro-fibers
US20090062870A1 (en) * 2007-08-27 2009-03-05 Giuseppe Milano Method of arthroscopic osteochondral resurfacing using prp strengthened with fibrin glue
CN101829361A (en) * 2009-03-10 2010-09-15 广州迈普再生医学科技有限公司 Nano-bionic material for tissue repair and preparation method thereof
CA2826771A1 (en) * 2012-09-13 2014-03-13 The Uab Research Foundation Biomimetic tissue graft for ligament replacement
WO2015162559A1 (en) * 2014-04-21 2015-10-29 Ineb - Instituto Nacional De Engenharia Biomédica Mesh composition for repairing or the regeneration of tissues and methods thereof
CN107349475A (en) * 2017-07-14 2017-11-17 中国人民解放军第四军医大学 Artificial organ engineering skin that nano fibrous membrane is layering with stem cell and preparation method thereof
CN108653328A (en) * 2018-03-26 2018-10-16 杭州市萧山区中医院 Application of the mesenchyma stem cell combined platelet rich plasma in preparing the drug for promoting the healing of rotator cuff injury tendon bone complex
CN110693629A (en) * 2019-11-19 2020-01-17 上海市第六人民医院 Sectional and gradient functional tendon sleeve implantation instrument and preparation method thereof
CN110898253A (en) * 2019-12-17 2020-03-24 浙江大学医学院附属第一医院 Method for preparing intrafiber biomimetic mineralized collagen membrane capable of promoting cell osteogenic differentiation
CN111346262A (en) * 2020-03-17 2020-06-30 四川大学 Injectable calcium-phosphorus ceramic for promoting healing of tendon and bone and preparation method and application thereof
CN112999424A (en) * 2021-02-08 2021-06-22 四川大学 Composite electrospun membrane for promoting healing of aponeurosis as well as preparation method and application thereof
CN113368308A (en) * 2021-06-07 2021-09-10 北京市创伤骨科研究所 Bionic sandwich-structured artificial periosteum and preparation method thereof

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2824875Y (en) * 2005-04-14 2006-10-11 南方医院 An injection type tissue engineering bone engraft
CN1846790A (en) * 2005-04-14 2006-10-18 南方医院 Injected tissue engineering bone and its constrction and application
US20080112998A1 (en) * 2006-11-14 2008-05-15 Hongjun Wang Innovative bottom-up cell assembly approach to three-dimensional tissue formation using nano-or micro-fibers
US20090062870A1 (en) * 2007-08-27 2009-03-05 Giuseppe Milano Method of arthroscopic osteochondral resurfacing using prp strengthened with fibrin glue
CN101829361A (en) * 2009-03-10 2010-09-15 广州迈普再生医学科技有限公司 Nano-bionic material for tissue repair and preparation method thereof
CA2826771A1 (en) * 2012-09-13 2014-03-13 The Uab Research Foundation Biomimetic tissue graft for ligament replacement
WO2015162559A1 (en) * 2014-04-21 2015-10-29 Ineb - Instituto Nacional De Engenharia Biomédica Mesh composition for repairing or the regeneration of tissues and methods thereof
CN107349475A (en) * 2017-07-14 2017-11-17 中国人民解放军第四军医大学 Artificial organ engineering skin that nano fibrous membrane is layering with stem cell and preparation method thereof
CN108653328A (en) * 2018-03-26 2018-10-16 杭州市萧山区中医院 Application of the mesenchyma stem cell combined platelet rich plasma in preparing the drug for promoting the healing of rotator cuff injury tendon bone complex
CN110693629A (en) * 2019-11-19 2020-01-17 上海市第六人民医院 Sectional and gradient functional tendon sleeve implantation instrument and preparation method thereof
CN110898253A (en) * 2019-12-17 2020-03-24 浙江大学医学院附属第一医院 Method for preparing intrafiber biomimetic mineralized collagen membrane capable of promoting cell osteogenic differentiation
CN111346262A (en) * 2020-03-17 2020-06-30 四川大学 Injectable calcium-phosphorus ceramic for promoting healing of tendon and bone and preparation method and application thereof
CN112999424A (en) * 2021-02-08 2021-06-22 四川大学 Composite electrospun membrane for promoting healing of aponeurosis as well as preparation method and application thereof
CN113368308A (en) * 2021-06-07 2021-09-10 北京市创伤骨科研究所 Bionic sandwich-structured artificial periosteum and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王迎军: "新型材料科学与技术 无机材料卷 下", 广州：华南理工大学出版社, pages: 1335 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949353A (en) * 2022-01-24 2022-08-30 东华大学 Biological function differentiated adaptation musculoskeletal system prosthesis and preparation method thereof
CN115068687A (en) * 2022-07-08 2022-09-20 重庆科技学院 Gradient nano/microfiber scaffold and preparation method and application thereof
CN115068687B (en) * 2022-07-08 2023-12-12 重庆科技学院 Gradient nano/micro fiber support and preparation method and application thereof

Similar Documents

Publication Publication Date Title
Guerado et al. Challenges of bone tissue engineering in orthopaedic patients
Baldino et al. Regeneration techniques for bone-to-tendon and muscle-to-tendon interfaces reconstruction
US8579947B2 (en) Polyporous hollow bone screw
US9592125B2 (en) In situ system for intra-articular chondral and osseous tissue repair
US20070102010A1 (en) Naso-pharyngeal tissue engineering
KR20050085079A (en) Treatment of tissue with undifferentiated mesenchymal cells
CN113893388A (en) Modular tissue engineering bone-ligament-bone graft and preparation method thereof
Emara et al. Recent update on craniofacial tissue engineering
Mao et al. A systematic review of tissue engineering scaffold in tendon bone healing in vivo
CN110743040B (en) Bionic skeletal muscle composite tissue prepared by multi-channel extrusion 3D biological printing
Yang et al. Strategies for promoting tendon-bone healing: Current status and prospects
Tian et al. Repair of urethral defects by an adipose mesenchymal stem cell‑porous silk fibroin material
Hao et al. Local injection of bone mesenchymal stem cells and fibrin glue promotes the repair of bone atrophic nonunion in vivo
Özdemir et al. Biological augmentation strategies in rotator cuff repair
Zhang et al. Three-dimensional bioprinting of a structure-, composition-, and mechanics-graded biomimetic scaffold coated with specific decellularized extracellular matrix to improve the tendon-to-bone healing
Sun et al. Application of 3D printing & 3D bioprinting for promoting cutaneous wound regeneration
CN104906633A (en) Injectable bone repair material and preparation method therefor
AU2018315622B2 (en) Multiphasic tissue scaffold constructs
Beri et al. Tissue engineering in maxillofacial region from past to present
Koçyiğit et al. Epidermal growth factor stimulates rabbit achilles tendon histologically and biomechanically healing
CN113995888B (en) Tissue engineering tendon and preparation method thereof
Kouroupis et al. Mesenchymal stem cell applications for ligament repair after joint trauma
Sheykhhasan Mesenchymal stem cells and platelet derived concentrates in regenerative medicine
Smith et al. Biologically active blood plasma-based biomaterials as a new paradigm for tissue repair therapies
US20220347347A1 (en) Ligament Repair Scaffold

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination