CN113881570B - High-density Bojioocyst algae culture medium - Google Patents
High-density Bojioocyst algae culture medium Download PDFInfo
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- CN113881570B CN113881570B CN202111175296.7A CN202111175296A CN113881570B CN 113881570 B CN113881570 B CN 113881570B CN 202111175296 A CN202111175296 A CN 202111175296A CN 113881570 B CN113881570 B CN 113881570B
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 34
- 239000001963 growth medium Substances 0.000 title abstract description 28
- 210000003250 oocyst Anatomy 0.000 claims abstract description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 14
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims abstract description 11
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 7
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000004115 Sodium Silicate Substances 0.000 claims abstract description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000365 copper sulfate Inorganic materials 0.000 claims abstract description 6
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229960002413 ferric citrate Drugs 0.000 claims abstract description 6
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 claims abstract description 6
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000019799 monosodium phosphate Nutrition 0.000 claims abstract description 6
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 6
- 235000015393 sodium molybdate Nutrition 0.000 claims abstract description 6
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 6
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000010344 sodium nitrate Nutrition 0.000 claims abstract description 6
- 239000004317 sodium nitrate Substances 0.000 claims abstract description 6
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052911 sodium silicate Inorganic materials 0.000 claims abstract description 6
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 6
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 6
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims abstract description 5
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims abstract description 5
- 235000002867 manganese chloride Nutrition 0.000 claims abstract description 5
- 239000011565 manganese chloride Substances 0.000 claims abstract description 5
- 229940099607 manganese chloride Drugs 0.000 claims abstract description 5
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims abstract description 4
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims abstract description 4
- 229930003779 Vitamin B12 Natural products 0.000 claims abstract 2
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims abstract 2
- 235000019163 vitamin B12 Nutrition 0.000 claims abstract 2
- 239000011715 vitamin B12 Substances 0.000 claims abstract 2
- 235000013343 vitamin Nutrition 0.000 claims description 6
- 229930003231 vitamin Natural products 0.000 claims description 6
- 239000011782 vitamin Substances 0.000 claims description 6
- 229940088594 vitamin Drugs 0.000 claims description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 230000000050 nutritive effect Effects 0.000 claims 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims 1
- 229910052748 manganese Inorganic materials 0.000 claims 1
- 239000011572 manganese Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract description 2
- 229930003756 Vitamin B7 Natural products 0.000 abstract description 2
- 150000008040 ionic compounds Chemical class 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 235000011912 vitamin B7 Nutrition 0.000 abstract description 2
- 239000011735 vitamin B7 Substances 0.000 abstract description 2
- 235000019794 sodium silicate Nutrition 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 11
- 239000002609 medium Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196319 Chlorophyceae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000195650 Oocystaceae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a high-density Bojioocyst algae culture medium, which comprises the following components: sodium nitrate, sodium dihydrogen phosphate, sodium silicate, copper sulfate, zinc sulfate, magnesium sulfate, cobalt chloride, manganese chloride, sodium molybdate, disodium ethylenediamine tetraacetate, ferric citrate, and vitamin B 1 Vitamin B12, vitamin H; the culture medium realizes the rapid proliferation, high density and good quality cultivation of the oocyst algae by optimizing the proportion of each component of the nutrient salt and adding important ionic compounds.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a culture medium formula capable of improving the culture concentration of oocyst algae.
Technical Field
The Bojia oocyst algae is belonging to Chlorophyta, chlorophyceae, chlorococcus, oocystaceae, oocysts, and oocystsOocystis. Algae cells generally live in groups of 2, 4 and 8 algae cells, and a layer of obvious saccular membrane is arranged outside the groups.
The Bojioocyst algae has the characteristics of stable growth, metal ion absorption, ammonia nitrogen and nitrite reduction, oxygen supply, water ecological balance promotion and the like, is an excellent aquaculture activity regulator, and has an important function in ecological culture. In recent years, the bojioocyst algae has been applied to healthy culture of litopenaeus vannamei, and good culture effect is obtained.
However, bogericystis cells grew slowly with F/2 and ASP 2 The traditional culture medium is used for culturing Bojioocyst algae, and the phenomena of overlong growth period, low cell concentration and the like are easy to occur. The growth period is too long, and algae cells are easy to decay, so that the quality of microalgae is reduced; meanwhile, bacteria, protozoa and other harmful organisms are easy to breed. In addition, the cell concentration is low, the cultivation requirement can not be met, the production resource is wasted, and the application of the oocyst algae in aquaculture is restricted.
Disclosure of Invention
For the above reasons, the applicant has obtained a high-density oocyst algae culture medium through many years of scientific research. The culture medium realizes the rapid proliferation, high density and good quality cultivation of the oocyst algae by optimizing the proportion of each component of the nutrient salt and adding important ionic compounds.
The invention is realized by the following technical scheme.
A high density botechin culture medium comprising: 0.1 to 0.2 weight part of sodium nitrate, 0.008 to 0.01 weight part of sodium dihydrogen phosphate, 0.01 to 0.02 weight part of sodium silicate, 0.00003 to 0.00005 weight part of copper sulfate, 0.00009 to 0.000095 weight part of zinc sulfate, 0.0001 to 0.0003 weight part of magnesium sulfate, 0.000045 to 0.00005 weight part of cobalt chloride, 0.00071 to 0.00072 weight part of manganese chloride, 0.000029 to 0.0000295 weight part of sodium molybdate, 0.017 to 0.018 weight part of disodium edetate, 0.015 to 0.016 weight part of ferric citrate and vitamin B 1 0.0003 to 0.0005 weight parts, vitamin B12.000001 to 0.000003 weight parts, and vitamin H0.000001 to 0.000003 weight parts.
The culture medium is added into a microalgae culture container to culture the microalgae solution.
Preferred such media include: 0.1496 parts by weight of sodium nitrate, 0.0088 part by weight of sodium dihydrogen phosphate, 0.015 part by weight of sodium silicate, 0.00004 part by weight of copper sulfate, 0.000092 parts by weight of zinc sulfate, 0.0002 part by weight of magnesium sulfate, 0.000048 parts by weight of cobalt chloride, 0.000712 parts by weight of manganese chloride, 0.0000292 parts by weight of sodium molybdate, 0.0174 part by weight of disodium ethylenediamine tetraacetate, 0.0156 part by weight of ferric citrate, and vitamin B 1 0.0004 parts by weight of vitamin B 12 0.000002 weight portion and 0.000002 weight portion of vitamin H.
The high-density oocyst algae culture medium is used for large-scale expansion culture of oocyst algae.
The high-density Bojioocyst algae culture medium can be cultured for 5 days to obtain Bojioocyst algae liquid with high density and good quality.
Compared with the traditional nutrient salt formula, the formula has the following advantages:
(1) The cell concentration is high. Under the same cultivation condition, the concentration of the nutrient medium algae cells is obviously higher than that of the traditional culture medium formula F/2. If the initial concentration is 28 ten thousand/ml, the new culture medium can reach more than 200 ten thousand/ml after 5 days, and the production requirement can be met.
(2) The growth speed is high. The culture medium of the invention is used for cultivating the oocyst algae, the growth of algae cells is rapid, and compared with the traditional nutrient salt F/2, the growth speed is 1.3 times of the original growth speed.
(3) The culture medium of the invention is added with a proper amount of magnesium sulfate, which can promote photosynthesis of the oocyst algae and increase accumulation of organic matters.
(4) The cell plumpness is high. Because the nitrogen and phosphorus element content of the culture medium is high, the component ratio of each element is more suitable for the growth of the oocyst algae, and the culture medium is superior to the traditional culture medium such as F/2 under the same proper culture condition.
(5) The content of harmful organisms such as bacteria and fungi is low. Due to the high efficiency of the new culture medium, the cultivation of the Bojioocyst algae solution can be completed in a short time, and the content of bacteria can be effectively reduced.
(6) Can reduce the pollution of the original activity. The high-efficiency culture property of the novel nutrient salt shortens the culture time of the oocyst algae. In the large-scale production process, the reduction of the cultivation period can effectively reduce the pollution possibility of protozoa.
Drawings
1. FIG. 1: cultivation concentration comparison graph of LY01 culture medium and F/2 culture medium.
2. Fig. 2: the Bojioocyst algae cultivated by the LY01 cultivation medium of the invention.
3. Fig. 3: oocyst algae cultivated in F/2 medium.
Detailed Description
Example 1
Each liter of medium comprises: 0.1496g of sodium nitrate, 0.0088g of sodium dihydrogen phosphate, 0.015g of sodium silicate, 0.00004g of copper sulfate, 0.000092g of zinc sulfate, 0.0002g of magnesium sulfate, 0.000048g of cobalt chloride, 0.000712 g g of manganese chloride, 0.0000292 g g of sodium molybdate, 0.0174 g g of disodium ethylenediamine tetraacetate, 0.0156 g of ferric citrate, vitamin B 1 0.0004 g, vitamin B 12 0.000002 g, vitamin H0.000002 and g.
Example 2
In a 250L acrylic barrel (the cultivation conditions are that the water temperature is 26 ℃, the illumination intensity is 6000lx, the light-dark period is 14h to 10h, the initial cultivation density of algae liquid is 28 ten thousand/ml) according to the following ratio of 1: f/2 nutrient salt and the culture medium of the invention of the example 1 are respectively added in a proportion of 1000, and the growth characteristics of the two are observed, wherein the data are as follows:
TABLE 1 growth traits of oocyst algae cultivated in different media
Experimental conclusion by cultivation comparison, the growth speed of the culture medium is 1.31 times that of the traditional culture medium F/2; the highest density (286 ten thousand/ml) of culture medium culture is obviously higher than that of F/2 culture medium (218 ten thousand/ml); the fullness of the cultured algae cells is obviously better than that of the F/2 culture medium (shown in fig. 2 and 3).
Embodiments include, but are not limited to, the above.
Claims (3)
1. A high-density oocyst algae nutritive salt is characterized in that the nutritive salt comprises the following components: 0.1 to 0.2 weight part of sodium nitrate, 0.008 to 0.01 weight part of sodium dihydrogen phosphate, 0.01 to 0.02 weight part of sodium silicate, 0.00003 to 0.00005 weight part of copper sulfate, 0.00009 to 0.000095 weight part of zinc sulfate, 0.0001 to 0.0003 weight part of magnesium sulfate, 0.000045 to 0.00005 weight part of cobalt chloride, 0.00071 to 0.00072 weight part of manganese chloride, 0.000029 to 0.0000295 weight part of sodium molybdate, 0.017 to 0.018 weight part of disodium edetate, 0.015 to 0.016 weight part of ferric citrate and vitamin B 1 0.0003 to 0.0005 weight parts, vitamin B12.000001 to 0.000003 weight parts, and vitamin H0.000001 to 0.000003 weight parts.
2. A high density oocyst algae nutritive salt according to claim 1, for use in: and adding nutritive salt into the microalgae cultivation container to cultivate the microalgae liquid.
3. The high-density oocyst algae nutritive salt of claim 1, comprising: 0.1496 parts by weight of sodium nitrate, 0.0088 part by weight of sodium dihydrogen phosphate, 0.015 part by weight of sodium silicate, 0.00004 part by weight of copper sulfate, 0.000092 parts by weight of zinc sulfate, 0.0002 part by weight of magnesium sulfate, 0.000048 parts by weight of cobalt chloride and 0.000712 parts by weight of manganese chloride0.0000292 parts by weight of sodium molybdate, 0.0174 part by weight of disodium ethylenediamine tetraacetate, 0.0156 part by weight of ferric citrate and vitamin B 1 0.0004 weight parts, vitamin B12 0.000002 weight parts, and vitamin H0.000002 weight parts.
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| CN202111175296.7A CN113881570B (en) | 2021-12-02 | 2021-12-02 | High-density Bojioocyst algae culture medium |
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| CN202111175296.7A CN113881570B (en) | 2021-12-02 | 2021-12-02 | High-density Bojioocyst algae culture medium |
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| CN113881570B true CN113881570B (en) | 2024-02-23 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820325A (en) * | 2014-03-03 | 2014-05-28 | 临沂大学 | High-density culture technology for oocystis borgei and collection method for oocystis borgei cells |
| CN112273218A (en) * | 2020-10-27 | 2021-01-29 | 湛江腾飞实业有限公司 | Method for indoor culture of composite algae by using nutrient salts |
| CN112574888A (en) * | 2020-12-10 | 2021-03-30 | 武汉市中易天地物联科技有限公司 | Culture medium for expanding culture of concentrated oocyst algae |
-
2021
- 2021-12-02 CN CN202111175296.7A patent/CN113881570B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820325A (en) * | 2014-03-03 | 2014-05-28 | 临沂大学 | High-density culture technology for oocystis borgei and collection method for oocystis borgei cells |
| CN112273218A (en) * | 2020-10-27 | 2021-01-29 | 湛江腾飞实业有限公司 | Method for indoor culture of composite algae by using nutrient salts |
| CN112574888A (en) * | 2020-12-10 | 2021-03-30 | 武汉市中易天地物联科技有限公司 | Culture medium for expanding culture of concentrated oocyst algae |
Non-Patent Citations (2)
| Title |
|---|
| Mg2+、Mn2+对波吉卵囊藻生长的影响;张慧 等;广东海洋大学学报;第第41卷卷(第第2期期);第60-68页 * |
| 陈明耀 等.生物饵料培养.中国农业出版社,1999,(第1版),第65、70-71页. * |
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