CN113875773B - Nanometer preparation for preventing and controlling wheat powdery mildew and preparation method thereof - Google Patents
Nanometer preparation for preventing and controlling wheat powdery mildew and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a nano preparation for preventing and controlling wheat powdery mildew, which relates to the technical field of prevention and control of wheat powdery mildew, and is mainly prepared from the following raw materials in percentage by weight: 1 per mill-1% (m/v) of loss control regulator, 1 per mill-30% (v/v) of simethicone, 1 per mill-1% (m/v) of gamma-irradiated nano montmorillonite, and the balance of water. The invention also provides a preparation method of the nano preparation. The invention has the beneficial effects that: compared with the prior art, the invention has the advantages that the wheat powdery mildew prevention and control effect is enhanced, the incidence rate of wheat powdery mildew can be reduced by more than 99% after wheat is treated, the normal field growth and propagation of the wheat are not influenced, and the safety and stability of the yield and the quality of the wheat are ensured. The invention has the advantages that: the powdery mildew inhibitor is natural, green, environment-friendly and efficient, and can be used for large-area field prevention and control of powdery mildew.
Description
Technical Field
The invention relates to the technical field of prevention and control of wheat powdery mildew, and particularly relates to a nano preparation for preventing and controlling wheat powdery mildew and a preparation method thereof.
Background
Wheat is the most widely grown food crop in the world, second only to maize, providing over 20% of the total energy and protein in the human diet. Wheat powdery mildew is a leaf disease caused by wheat powdery mildew (Blumeria graminis f.sp.tritici) and is prevalent in warm and high-humidity areas of the world. Wheat powdery mildew is a worldwide disease that seriously affects wheat yield and quality. Powdery mildew damages all organs of overground wheat plants, but mainly damages wheat leaves and She Shu, when powdery mildew infects wheat, powdery mildew spores cover the surfaces of the wheat leaves, so that chlorophyll of the wheat is degraded, the photosynthetic area is reduced, the photosynthetic efficiency is reduced, and the yield loss is 5% -34% during disease attack.
At present, the occurrence of wheat powdery mildew is mainly controlled by breeding disease-resistant varieties and using chemical bactericides. Expression of pathogenicity-related (PR) proteins is closely associated with increased disease resistance in plants. Induction of PR gene expression in arabidopsis, rice and soybean confers resistance to different pathogens. This means that PR gene can be used as a candidate gene for improving wheat resistance. However, due to the change of physiological species of pathogenic bacteria, the resistance of the variety is easy to lose, and the resistance of the production variety can be lost after 3-5 years, so that the spraying of the bactericide is still an effective measure for preventing and controlling powdery mildew. The prevention and treatment of powdery mildew in China mainly takes triazolone as a main material. With the increase of the amount of the bactericide, the quality of the wheat is deteriorated, the protein content is reduced, the amount is reduced, and the bread volume is reduced. The use of fungicides has an influence on environmental safety with an increase in pesticide residues. Therefore, people urgently want to develop a green, efficient and harmless product for preventing and treating powdery mildew in wheat production.
The patent with publication number CN107371913A discloses a wheat powdery mildew inhibitor and a preparation method thereof, wherein the inhibitor comprises a regulation and control agent, modified silicone oil, nano-selenium and water; the loss control regulating agent is formed by mixing aminated attapulgite and micro-nano biological silicon/carbon according to the mass ratio of (3-5) to (1-2), but the inhibition effect of wheat powdery mildew is still to be improved after the inhibitor is treated.
Disclosure of Invention
The invention aims to solve the technical problem that the inhibition effect of wheat powdery mildew in the prior art still needs to be improved, and provides a nano isolating membrane for preventing and controlling wheat powdery mildew and a preparation method thereof.
The invention solves the technical problems through the following technical means:
a nanometer preparation for preventing and controlling wheat powdery mildew is mainly prepared from the following raw materials in percentage by weight: 1 per mill-1% (m/v) of loss control regulating agent, 1 per mill-30% (v/v) of simethicone, 1 per mill-1% (m/v) of gamma-irradiated nano montmorillonite, and the balance of water;
the preparation method of the loss control regulating agent comprises the following steps:
(1) Taking oxygen and ammonia gas as gas sources, performing intermittent irradiation treatment on 100-1500 mesh adsorption grade attapulgite powder under a vacuum condition by using plasma with the temperature of 500-1000 ℃ and the power of 1-3KW for 1-20 minutes, stopping for 1-20 minutes until the total effective irradiation time reaches 60-120 minutes, and preparing the aminated attapulgite;
(2) Under the anaerobic condition of nitrogen protection, performing pyrolysis on rice straws at 500-800 ℃ for 50-100 minutes by adopting a muffle furnace to obtain micro-nano biological silicon/carbon;
(3) Uniformly mixing the aminated attapulgite and the micro-nano biological silicon/carbon according to the mass ratio of 3-5:1-2 to obtain the disorder control agent.
Has the advantages that: compared with the prior art, the invention has the advantages that the wheat powdery mildew prevention and control effect is enhanced, the incidence of powdery mildew can be reduced by more than 99 percent after spraying, the cost is reduced to half, and the environment is more friendly.
According to the invention, the dimethyl silicone oil is matched with the material of the nano-montmorillonite, and after the nano-montmorillonite is sprayed on the wheat leaves, the isolation film is formed on the surfaces of the wheat leaves, and after the nano-montmorillonite after gamma irradiation is added, the uniformity and the durability of the isolation film can be improved, the action effect of the nano-isolation film is improved, the identification of wheat powdery mildew fungal spores and a wax layer on the surfaces of wheat can be efficiently blocked, the spore germination process of wheat powdery mildew is inhibited, wheat powdery mildew can be effectively prevented, and the grain safety is ensured.
When the percentage of each raw material is lower than the range, no obvious prevention and control effect is achieved, when the percentage of each raw material exceeds the range, the prevention and control effect cannot be increased, the prevention and control cost is increased, and the wheat growth is affected due to yellowing of leaves when the raw materials are used at an excessively high concentration.
The quality of montmorillonite can not be obviously changed after gamma irradiation, but the space lamellar gap of montmorillonite is changed after gamma ray irradiation, so that the sterilization effect of montmorillonite is enhanced, and the simethicone and the nano montmorillonite after gamma ray irradiation have synergistic effect, so that the combined infection of powdery mildew on leaves can be reduced. The disorder control agent has the function of controlling loss, improves the action time of the nanometer isolating membrane and enhances the prevention and control effect on powdery mildew.
Preferably, the volume ratio of the oxygen gas to the ammonia gas is 1:1-5.
The preparation method of the nano preparation for preventing and controlling wheat powdery mildew comprises the following steps:
(1) Irradiating the nano montmorillonite with a high-energy electron beam accelerator with the dose of 10-400kGy for 1-24h to obtain gamma-irradiated nano montmorillonite;
(2) And (3) oscillating and mixing the misadjustment control agent, the simethicone, the gamma-irradiated nano montmorillonite and water to obtain the nano preparation for preventing and controlling the wheat powdery mildew.
Has the advantages that: the preparation method of the nano preparation is simple, convenient, green and safe, can be operated on a large scale, and has low cost, compared with the prior art, the prepared nano preparation has the advantages of enhanced wheat powdery mildew prevention and control effect, half of cost reduction and environmental friendliness.
Preferably, in the step (2), the loss control regulating agent is mixed with water to prepare a nano suspension, and then the nano suspension is mixed with simethicone and gamma-irradiated nano montmorillonite.
Preferably, the step (2) is performed for 5-60min by shaking at the rotating speed of 400-6000 rpm.
Preferably, the obtained nano preparation for preventing and controlling wheat powdery mildew is sprayed on wheat leaves to form a nano isolating membrane.
The invention has the advantages that: compared with the prior art, the invention has the advantages that the wheat powdery mildew prevention and control effect is enhanced, the incidence of powdery mildew can be reduced by more than 99 percent after spraying, the cost is reduced to half, and the environment is more friendly.
According to the invention, the dimethyl silicone oil is matched with the material of the nano-montmorillonite, and after the nano-montmorillonite is sprayed on the wheat leaves, the isolation film is formed on the surfaces of the wheat leaves, and after the nano-montmorillonite after gamma irradiation is added, the uniformity and the durability of the isolation film can be improved, the action effect of the nano-isolation film is improved, the identification of wheat powdery mildew fungal spores and a wax layer on the surfaces of wheat can be efficiently blocked, the spore germination process of wheat powdery mildew is inhibited, wheat powdery mildew can be effectively prevented, and the grain safety is ensured.
When the percentage of each raw material is lower than the range, no obvious prevention and control effect is achieved, when the percentage of each raw material exceeds the range, the prevention and control effect cannot be increased, the prevention and control cost is increased, and the wheat growth is affected due to yellowing of leaves when the raw materials are used at an excessively high concentration.
The quality of montmorillonite can not be obviously changed after gamma irradiation, but the space lamellar gap of montmorillonite is changed after gamma ray irradiation, so that the sterilization effect of montmorillonite is enhanced, and the simethicone and the nano montmorillonite after gamma ray irradiation have synergistic effect, so that the combined infection of powdery mildew on leaves can be reduced. The disorder control agent has the function of controlling loss, improves the action time of the nanometer isolating membrane and enhances the prevention and control effect on powdery mildew.
The preparation method of the nano preparation is simple, convenient, green and safe, can be operated on a large scale, and has low cost, compared with the prior art, the prepared nano preparation has the advantages of enhanced wheat powdery mildew prevention and control effect, half of cost reduction and environmental friendliness.
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FIG. 1 is a graph showing the results of treating wheat seedling stage leaves with the nano-formulations of comparative example 1 and example 1 according to the present invention;
FIG. 2 is a germination electron microscope image of Blumeria graminis spores on leaf surfaces after treatment of wheat seedling leaves by the nano-preparation in comparative example 1 and example 1 of the invention;
FIG. 3 is a graph showing the results of treating the leaf blades of wheat in the seedling stage with the nano-formulations of comparative example 1 to comparative example 3 and example 1;
FIG. 4 is a graph showing the results of the disease index measurement of wheat treated with the nano-formulations of comparative examples 1 to 3 and example 1 according to the present invention;
in the figure: a is comparative example 1,b, comparative example 2,c, comparative example 3,d, example 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The test materials and reagents used in the following examples, etc., are commercially available unless otherwise specified.
Those skilled in the art who do not specify any particular technique or condition in the examples can follow the techniques or conditions described in the literature in this field or follow the product specification.
Example 1
The nanometer preparation for preventing and controlling wheat powdery mildew is prepared from the following raw materials in percentage by weight: 1 per mill (m/v) of loss control regulating agent, 2 per mill (v/v) of simethicone, 1 per mill (m/v) of nano montmorillonite after gamma irradiation, and the balance of water.
The preparation method of the nanometer preparation for preventing and controlling wheat powdery mildew specifically comprises the following steps:
(1) Taking oxygen and ammonia (oxygen: ammonia (v/v) = 1:5) as gas sources, carrying out intermittent irradiation treatment (treatment for 10 minutes and stopping for 10 minutes) on attapulgite powder (800 meshes, adsorption grade) by using plasma (500 ℃, 2 kw) under a vacuum condition, wherein the total effective irradiation time reaches 60 minutes, and then, amino groups can be grafted on the surface of the attapulgite crystal to prepare aminated attapulgite;
(2) Under the anaerobic condition of nitrogen protection, performing pyrolysis (800 ℃) on rice straws for 50 minutes by adopting a muffle furnace to obtain micro-nano biological silicon/carbon;
(3) Uniformly mixing the aminated attapulgite and the micro-nano biological silicon/carbon according to the mass ratio of 3:1 to obtain a control disorder control agent;
(4) Carrying out irradiation treatment on the nano montmorillonite for 24 hours by using a high-energy electron beam accelerator with the dose of 300kGy to obtain gamma-irradiated nano montmorillonite;
(5) And (3) fully shaking the loss control regulating agent in percentage with water (processing for 10 minutes at the rotating speed of 3000 rpm) to prepare nano suspension, mixing the nano suspension with the simethicone and the gamma-irradiated nano montmorillonite, and fully shaking (processing for 20 minutes at the rotating speed of 3000 rpm) to prepare the nano preparation.
Treatment of potted wheat:
(1) The leaves of potted wheat are sprayed evenly in fine mist for 1 time, and the dosage is based on the fact that the sprayed parts are all wet.
(2) A large number of powdery mildew spores (Blumeria graminis f.sp.Tritici, original species provided by professor Wang Xiue, nanjing university of agriculture, university of Nanjing, well known as the common materials Two members of Tarlk family friction specific labor resistance in common where BMC Plant Biology (2016) (27) 16) were cultured in laboratory incubators, diluted to 1000U/mL of powdery mildew spore suspension for inoculation of powdery mildew spores in the field.
(3) The wheat after spraying treatment was sprayed with powdery mildew spores (50L per acre).
(4) Counting the incidence of the potted wheat 7-15 days after the treatment. (fifty leaves per treatment, disease index DI,
wherein, the disease level of I (0-100%), xi is the number of leaves with the disease level of I, si is the severity value of the disease level of I, and I is the disease incidence.
Statistics shows that the embodiment can reduce the incidence rate of wheat powdery mildew by over 99 percent and effectively reduce the harm of the powdery mildew to the yield and the quality of wheat.
Example 2
The nanometer preparation for preventing and controlling wheat powdery mildew is prepared from the following raw materials in percentage by weight: 1 per mill (m/v) of loss control regulating agent, 5 per mill (v/v) of simethicone, 1 per mill (m/v) of nano montmorillonite after gamma irradiation, and the balance of water.
The preparation method of the nanometer preparation for preventing and controlling wheat powdery mildew specifically comprises the following steps:
(1) Using oxygen and ammonia (oxygen: ammonia (v/v) = 1:5) as gas sources, carrying out intermittent irradiation treatment (treatment for 10 minutes and stopping for 10 minutes) on attapulgite powder (800 meshes, adsorption grade) by using plasma (500 ℃, 2 kw) under a vacuum condition, wherein the total effective irradiation time reaches 60 minutes, and then, grafting amino on the surface of the attapulgite rod crystal to prepare aminated attapulgite;
(2) Under the anaerobic condition of nitrogen protection, performing pyrolysis (800 ℃) on rice straws for 50 minutes by adopting a muffle furnace to obtain micro-nano biological silicon/carbon;
(3) Uniformly mixing the aminated attapulgite and the micro-nano biological silicon/carbon according to the mass ratio of 3:1 to obtain a control disorder control agent;
(4) Performing irradiation treatment on the nano montmorillonite for 20 hours by using a high-energy electron beam accelerator with the dose of 400kGy to obtain gamma-irradiated nano montmorillonite;
(5) And (3) fully shaking the loss control regulating agent and water (processing at the rotating speed of 3000rpm for 10 minutes) and uniformly mixing to prepare a nano suspension, mixing the nano suspension with simethicone and gamma-irradiated nano montmorillonite, and fully shaking (processing at the rotating speed of 3000rpm for 20 minutes) and uniformly mixing to prepare the nano preparation.
And (3) field wheat green leaf period treatment:
(1) And (3) uniformly spraying the wheat leaves in the green leaf period in a fine mist manner for 1 time, wherein the dosage is based on that the sprayed parts are completely wet.
(2) Culturing a large amount of powdery mildew spores in a laboratory incubator, and diluting into 1000U/mL powdery mildew spore suspension for inoculation of the field powdery mildew spores.
(3) The wheat after spraying was sprayed with the same amount of powdery mildew spores as in example 1.
(4) The statistical method for the field wheat disease condition 15-45 days after the treatment is the same as that of the example 1.
Statistics shows that the embodiment can reduce the incidence rate of wheat powdery mildew by over 99 percent and effectively reduce the harm of the powdery mildew to the yield and the quality of wheat.
Example 3
The nanometer preparation for preventing and controlling wheat powdery mildew is prepared from the following raw materials in percentage by weight: 5 per mill (m/v) of a disorder control agent, 8 percent (v/v) of simethicone, 5 per mill (m/v) of nano montmorillonite after gamma irradiation, and the balance of water.
The preparation method of the nanometer preparation for preventing and controlling wheat powdery mildew specifically comprises the following steps:
(1) Taking oxygen and ammonia (oxygen: ammonia (v/v) = 1:5) as gas sources, carrying out intermittent irradiation treatment (treatment for 15 minutes and stopping for 15 minutes) on attapulgite powder (800 meshes, adsorption grade) by using plasma (500 ℃, 2 kw) under a vacuum condition, wherein the total effective irradiation time reaches 70 minutes, and then, amino groups can be grafted on the surface of the attapulgite crystal to prepare aminated attapulgite;
(2) Under the anaerobic condition of nitrogen protection, performing pyrolysis (800 ℃) on rice straws for 50 minutes by adopting a muffle furnace to obtain micro-nano biological silicon/carbon;
(3) Uniformly mixing the aminated attapulgite and the micro-nano biological silicon/carbon according to the mass ratio of 3:1 to obtain a control disorder control agent;
(4) Carrying out irradiation treatment on the nano montmorillonite for 22h by using a high-energy electron beam accelerator with the dose of 350kGy to obtain gamma-irradiated nano montmorillonite;
(5) And (3) fully shaking the loss control regulating agent and water (processing for 15 minutes at the rotating speed of 3500 rpm) to prepare a nano suspension, mixing the nano suspension with simethicone and gamma-irradiated nano montmorillonite, and fully shaking (processing for 30 minutes at the rotating speed of 4000 rpm) to prepare a nano preparation.
And (3) field wheat green leaf period treatment:
(1) And (3) uniformly spraying the wheat leaves in the green leaf period in a fine mist manner for 1 time, wherein the dosage is based on that the sprayed parts are completely wet.
(2) Culturing a large amount of powdery mildew spores in a laboratory incubator, and diluting into 1000U/mL powdery mildew spore suspension for inoculation of the field powdery mildew spores.
(3) The wheat after spraying was sprayed with the same amount of powdery mildew spores as in example 1.
(4) The statistical method for the field wheat disease condition 15-45 days after the treatment is the same as that of the example 1.
Statistics shows that the embodiment can reduce the incidence rate of wheat powdery mildew by over 99 percent and effectively reduce the harm of the powdery mildew to the yield and the quality of wheat.
Example 4
This embodiment is different from embodiment 1 in that: the nanometer preparation for preventing and controlling wheat powdery mildew is mainly prepared from the following raw materials in percentage by weight: 1% (m/v) of loss control regulating agent, 15% (v/v) of simethicone, 1% (m/v) of gamma-irradiated nano montmorillonite and the balance of water.
Comparative example 1
The comparative example is different from example 1 in that the nano-formulation for preventing and controlling wheat powdery mildew was not sprayed.
Comparative example 2
The present comparative example differs from comparative example 1 in that: the nanometer preparation for preventing and controlling wheat powdery mildew only contains 1 per mill (m/v) of nanometer montmorillonite irradiated by gamma and water.
Comparative example 3
The present comparative example differs from comparative example 1 in that: the nanometer preparation for preventing and controlling wheat powdery mildew only contains 2% (v/v) of simethicone and water.
FIG. 1 is a graph showing the results of the treatment of seedling stage leaves of wheat with the nano-preparations of comparative example 1 and example 1, wherein the control is not the spraying control of the nano-isolation film, and the surface of the leaves is covered with powdery mildew colonies after seven days, so that the bacterial plaque is obvious and the powdery mildew grows vigorously. The wheat of the treatment group is prevented and controlled by spraying the nano isolating membrane, so that the signal contact germination of powdery mildew spores and a waxy layer of a wheat leaf is effectively prevented, the germination and differentiation of wheat powdery mildew are obviously inhibited, the growth state of the leaf is excellent, and no obvious powdery mildew colonies and bacterial plaques exist.
FIG. 2 is a germination electron microscope image of Blumeria necator spores on the surfaces of leaves after the leaves of wheat in seedling stage are treated by the nano-preparation in comparative example 1 and example 1, and it can be seen that the control group shows that the Blumeria necator spores on the surfaces of the leaves of wheat are rich and germinate vigorously. Scanning electron microscope images of the treatment groups show that powdery mildew spores on the surfaces of the wheat leaves are rare, and germination and growth of the powdery mildew spores are inhibited.
Fig. 3 and 4 are graphs of results of treating wheat seedling stage leaves with the nano-formulation in comparative example 1 to comparative example 3 and example 1, respectively, and a disease index measurement result. The disease index of the control group is 95.8; the disease index of the nanometer montmorillonite after gamma irradiation is 54.33; the disease index after the treatment of the methyl silicone oil is 2.3; the disease index after the superposition treatment is 0.54, and the disease index is reduced by 99.46 percent compared with the control group. It can be seen that the combination of the simethicone and the gamma-irradiated nano-montmorillonite can reduce the combined infection of powdery mildew on leaves, and the simethicone and the nano-montmorillonite generate a synergistic effect.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. An application of a nano preparation in preventing and controlling wheat powdery mildew is characterized in that: the nano preparation is mainly prepared from the following raw materials in percentage by weight: the mass volume ratio of the control disorder control agent is 1 per thousand-1%, the volume ratio of the dimethyl silicone oil is 1 per thousand-30%, the mass volume ratio of the nano montmorillonite after gamma irradiation is 1 per thousand-1%, and the balance is water;
the preparation method of the loss control regulating agent comprises the following steps:
(1) Taking oxygen and ammonia gas as gas sources, performing intermittent irradiation treatment on 100-1500 mesh adsorption grade attapulgite powder by using plasma with the temperature of 500-1000 ℃ and the power of 1-3KW under a vacuum condition for 1-20 minutes, stopping for 1-20 minutes until the total effective irradiation time reaches 60-120 minutes, and preparing the aminated attapulgite;
(2) Under the anaerobic condition of nitrogen protection, performing pyrolysis on rice straws at 500-800 ℃ for 50-100 minutes by adopting a muffle furnace to obtain micro-nano biological silicon/carbon;
(3) Uniformly mixing the aminated attapulgite and the micro-nano biological silicon/carbon according to the mass ratio of 3-5:1-2 to obtain the disorder control agent.
2. The use of the nano-formulation according to claim 1 for the prevention and control of wheat powdery mildew, characterized in that: the volume ratio of the oxygen to the ammonia is 1:1-5.
3. The use of the nano-formulation according to claim 1 for the prevention and control of wheat powdery mildew, wherein the nano-formulation comprises: the mass ratio of the aminated attapulgite to the micro-nano biological silicon/carbon is 3:1.
4. Use of a nano-formulation according to any of claims 1 to 3 for the prevention and control of wheat powdery mildew, characterized in that: the preparation method of the nano preparation comprises the following steps:
(1) Irradiating the nano montmorillonite with a high-energy electron beam accelerator with the dose of 10-400kGy for 1-24h to obtain gamma-irradiated nano montmorillonite;
(2) And (3) oscillating and mixing the misadjustment control agent, the simethicone, the gamma-irradiated nano montmorillonite and water to obtain the nano preparation for preventing and controlling the wheat powdery mildew.
5. The use of the nano-formulation according to claim 4 for the prevention and control of wheat powdery mildew, characterized in that: in the step (2), the loss control regulating agent is mixed with water to prepare a nano suspension, and then the nano suspension is mixed with the simethicone and the gamma-irradiated nano montmorillonite.
6. The use of the nano-formulation according to claim 4 for the prevention and control of wheat powdery mildew, characterized in that: in the step (2), the shaking treatment is carried out for 5-60min at the rotating speed of 400-6000 rpm.
7. The use of the nano-formulation according to claim 4 for the prevention and control of wheat powdery mildew, characterized in that: and spraying the obtained nano preparation for preventing and controlling the wheat powdery mildew on wheat leaves to form a nano isolating membrane.
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| WO2008031512A1 (en) * | 2006-09-15 | 2008-03-20 | Bayer Cropscience Aktiengesellschaft | Oil-based suspension concentrates |
| CN111793433A (en) * | 2020-07-23 | 2020-10-20 | 中国科学院合肥物质科学研究院 | Nano seed coating agent and preparation method thereof |
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| CN102578082B (en) * | 2012-01-12 | 2014-08-13 | 中国科学院合肥物质科学研究院 | Method for controlling loss of pesticide at high efficiency and low cost |
| CN103355291A (en) * | 2013-05-10 | 2013-10-23 | 中国科学院合肥物质科学研究院 | Novel efficient method for improving adhesive capability of pesticide |
| CN105454288A (en) * | 2016-01-11 | 2016-04-06 | 济南康众医药科技开发有限公司 | Pesticide prepared from montmorillonite and used for preventing and controlling crop diseases |
| CN107371913B (en) * | 2017-07-28 | 2021-02-19 | 中国科学院合肥物质科学研究院 | Wheat powdery mildew inhibitor and preparation method thereof |
| CN108850004A (en) * | 2018-07-31 | 2018-11-23 | 深圳绿欣农业环保科技有限公司 | Compound plant source nano pesticide and preparation method thereof |
| CN110839618B (en) * | 2019-11-18 | 2022-09-13 | 浙江中环农业开发有限公司 | Nano protective agent for improving stress resistance of crops and preparation method thereof |
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| WO2008031512A1 (en) * | 2006-09-15 | 2008-03-20 | Bayer Cropscience Aktiengesellschaft | Oil-based suspension concentrates |
| CN111793433A (en) * | 2020-07-23 | 2020-10-20 | 中国科学院合肥物质科学研究院 | Nano seed coating agent and preparation method thereof |
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