CN113874514A - 超高精度病毒载体测定法 - Google Patents

超高精度病毒载体测定法 Download PDF

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Publication number
CN113874514A
CN113874514A CN202080038925.9A CN202080038925A CN113874514A CN 113874514 A CN113874514 A CN 113874514A CN 202080038925 A CN202080038925 A CN 202080038925A CN 113874514 A CN113874514 A CN 113874514A
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cells
viral
normal human
human cells
assay
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Chinese (zh)
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J·迈克南
H·佩尔托宁
M·哈西宁
S·伊拉－赫图拉
N·帕克尔
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Trizell Ltd
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Trizell Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
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  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202080038925.9A 2019-05-30 2020-05-22 超高精度病毒载体测定法 Pending CN113874514A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/426,124 2019-05-30
US16/426,124 US12152281B2 (en) 2019-05-30 2019-05-30 Gene therapy vector contamination assay
PCT/US2020/034146 WO2020242913A1 (en) 2019-05-30 2020-05-22 Ultra-high-precision viral vector assay

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CN113874514A true CN113874514A (zh) 2021-12-31

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US (2) US12152281B2 (enExample)
EP (1) EP3976803A4 (enExample)
JP (2) JP7755490B2 (enExample)
KR (1) KR20220016071A (enExample)
CN (1) CN113874514A (enExample)
AU (1) AU2020283526A1 (enExample)
BR (1) BR112021024020A2 (enExample)
CA (1) CA3140145A1 (enExample)
IL (1) IL288404B2 (enExample)
WO (1) WO2020242913A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12152281B2 (en) 2019-05-30 2024-11-26 Trizell Ltd. Gene therapy vector contamination assay
CN111041131B (zh) * 2020-03-16 2020-07-10 广东永诺医疗科技有限公司 基于微滴式数字pcr的eb病毒检测试剂盒

Citations (7)

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WO2003104476A2 (en) * 2002-06-07 2003-12-18 Novartis Ag Assay to detect replication competent viruses
CN102134613A (zh) * 2010-12-31 2011-07-27 中国人民解放军第三军医大学 重组慢病毒滴度的检测方法
EP2659002A1 (en) * 2010-12-27 2013-11-06 Ibis Biosciences, Inc. Quantitating high titer samples by digital pcr
GB201607596D0 (en) * 2016-04-29 2016-06-15 Nat Univ Ireland A multiplex assay for the sensitive and specific detection and differentiation of Clostridium difficile
CN107794291A (zh) * 2017-04-10 2018-03-13 中山大学 一种抗新加坡石斑鱼虹彩病毒的药物筛选方法
CN108570449A (zh) * 2018-05-17 2018-09-25 石河子大学 一种带有自发性kshv裂解复制的神经元细胞的构建方法
CN109072252A (zh) * 2016-04-14 2018-12-21 崔泽尔有限公司 大规模pei介导的质粒转染

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US6020191A (en) 1997-04-14 2000-02-01 Genzyme Corporation Adenoviral vectors capable of facilitating increased persistence of transgene expression
AU1817401A (en) * 1999-12-14 2001-06-25 Genovo, Incorporated Methods and compositions for the manufacture of replication incompetent adenovirus
US20080193484A1 (en) 2004-10-25 2008-08-14 Biogen Idec Ma Inc. Novel Methods for Producing Adenoviral Vector Preparations with Reduced Replication-Competent Adenovirus Contamination and Novel Adenoviral Vectors and Preparations
WO2008079354A2 (en) 2006-12-22 2008-07-03 Schering Corporation Methods of detecting replication competent adenovirus
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WO2003104476A2 (en) * 2002-06-07 2003-12-18 Novartis Ag Assay to detect replication competent viruses
EP2659002A1 (en) * 2010-12-27 2013-11-06 Ibis Biosciences, Inc. Quantitating high titer samples by digital pcr
CN102134613A (zh) * 2010-12-31 2011-07-27 中国人民解放军第三军医大学 重组慢病毒滴度的检测方法
CN109072252A (zh) * 2016-04-14 2018-12-21 崔泽尔有限公司 大规模pei介导的质粒转染
GB201607596D0 (en) * 2016-04-29 2016-06-15 Nat Univ Ireland A multiplex assay for the sensitive and specific detection and differentiation of Clostridium difficile
CN107794291A (zh) * 2017-04-10 2018-03-13 中山大学 一种抗新加坡石斑鱼虹彩病毒的药物筛选方法
CN108570449A (zh) * 2018-05-17 2018-09-25 石河子大学 一种带有自发性kshv裂解复制的神经元细胞的构建方法

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BR112021024020A2 (pt) 2022-04-12
WO2020242913A1 (en) 2020-12-03
CA3140145A1 (en) 2020-12-03
US20190292609A1 (en) 2019-09-26
JP2022534483A (ja) 2022-08-01
IL288404B1 (en) 2024-11-01
EP3976803A4 (en) 2023-11-22
US20250163519A1 (en) 2025-05-22
JP7755490B2 (ja) 2025-10-16
KR20220016071A (ko) 2022-02-08
US12152281B2 (en) 2024-11-26
EP3976803A1 (en) 2022-04-06
IL288404A (en) 2022-01-01
JP2025123368A (ja) 2025-08-22
AU2020283526A1 (en) 2021-12-23
IL288404B2 (en) 2025-03-01

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