CN113866096A - Pretreatment pipe and method for generating tubercle bacillus liquid-based detection sample - Google Patents
Pretreatment pipe and method for generating tubercle bacillus liquid-based detection sample Download PDFInfo
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- CN113866096A CN113866096A CN202111302965.2A CN202111302965A CN113866096A CN 113866096 A CN113866096 A CN 113866096A CN 202111302965 A CN202111302965 A CN 202111302965A CN 113866096 A CN113866096 A CN 113866096A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention relates to a pretreatment pipe for generating a tubercle bacillus liquid-based detection sample and a pretreatment method thereof, wherein the pretreatment pipe comprises an inner layer pipe and an outer layer pipe, the inner layer pipe is sleeved in the outer layer pipe, and an interlayer for accommodating the tubercle bacillus liquid-based detection sample is formed between the outer wall of the inner layer pipe and the inner wall of the outer layer pipe; the bottom of the inner-layer pipe is provided with a filter layer. The liquid enriched with the tubercle bacillus obtained by the method can be used for directly observing the tubercle bacillus by taking pictures by high-definition imaging equipment, and solves the problems of poor observation effect and complex process of the existing tubercle bacillus.
Description
Technical Field
The invention relates to the technical field of medical treatment, in particular to a pretreatment tube for generating a tubercle bacillus liquid-based detection sample and a pretreatment method thereof.
Background
Tuberculosis is a chronic infectious disease caused by tubercle bacillus infection, is also called tuberculosis and 'white plague', is an ancient infectious disease, is one of infectious diseases which are most threatening to human beings in the world at present, is the disease with the highest death rate among the infectious diseases caused by single cause, is the first infectious killer in developing countries, and becomes a great public health problem and a social problem.
The rapid development of scientific technology brings many new methods for tuberculosis experimental diagnosis, but bacteriological examination still remains an important means for tuberculosis experiment and diagnosis due to the advantages of objectivity, intuition, simplicity, low price and the like. At present, the main methods for diagnosing tuberculosis, discovering sources of infection, observing curative effects, determining whether to cure or not and performing epidemiological investigation and statistics in various countries in the world are smear dyeing of sputum of a patient, microscopic examination is performed, the number of tubercle bacillus in a sputum smear microscopic image is searched to be used as a main judgment basis for disease conditions, but the sputum is sticky and uneven in distribution of the tubercle bacillus, the sputum on a slide is possibly free of the tubercle bacillus, a plurality of pieces of sputum are required to be repeatedly observed to obtain results, and the sputum is rich in impurities, so that the sputum is only suitable for microscopic observation and cannot be used for high-definition imaging equipment to perform image shooting and image recognition, and the observation effect of the tubercle bacillus is poor.
The design of a tubercle bacillus liquid-based pretreatment method which can be used for high-definition imaging equipment and can directly shoot and identify tubercle bacillus is a problem which needs to be solved urgently by researchers.
Disclosure of Invention
The invention aims to provide a pretreatment pipe for generating a tubercle bacillus liquid-based detection sample and a pretreatment method thereof.
The invention provides a pretreatment pipe for generating a tubercle bacillus liquid-based detection sample, which comprises an inner layer pipe and an outer layer pipe, wherein the inner layer pipe is sleeved in the outer layer pipe, and an interlayer for accommodating the tubercle bacillus liquid-based detection sample is formed between the outer wall of the inner layer pipe and the inner wall of the outer layer pipe; the bottom of the inner-layer pipe is provided with a filter layer.
Further, the filter layer is a hydrophobic layer with filter holes.
Further, the diameter of the filter hole on the filter layer is 10
-20
。
Further, the filtering layer protrudes downwards, and the pipe diameter of the protruding part is gradually reduced from top to bottom.
Further, the filtering layer is in an inverted conical shape.
Further, the filtering layer is in an inverted hemispherical shape.
Further, the inner bottom surface of the outer layer pipe protrudes downward, and the inner diameter of the protruding portion is gradually reduced from top to bottom.
Further, the pretreatment method is realized by using the pretreatment tube according to any one of claims 1 to 7, and comprises the following steps:
step S1, placing the inner tube into the outer tube, adding the sputum and the sputum digestive juice into the inner tube, and fully mixing;
step S2, enabling the sputum and the sputum digestive juice to be fully mixed in the inner-layer tube (1), promoting the sputum to be rapidly and fully digested, separating the tubercle bacillus liquid-based detection sample from the digested sputum to the interlayer, and then taking away the inner-layer tube (1);
and step S3, adding a staining agent into the outer layer tube to stain the liquid-based detection sample.
Further, the S2 further includes:
and step S22, promoting sputum digestion and tubercle bacillus separation by ultrasonic oscillation.
And step S23, taking out the inner tube, and separating the tubercle bacillus liquid-based detection sample from the digested sputum to the interlayer through the filter layer of the inner tube.
Has the advantages that: the tubercle bacillus that this scheme obtained uses the liquid base as the carrier, and the tubercle bacillus is through enrichment and evenly distributed in the liquid base, can directly be used for high definition imaging device water conservancy diversion to image sensor chip on, carries out image capture and image recognition, not only images clearly be convenient for observe, and it is convenient to wash, only need simply wash can, can not cause the harm to the chip.
Drawings
FIG. 1 is a schematic view of the structure of the centrifugal tube of the present invention.
FIG. 2 is a schematic view of the structure of the outer tube.
Fig. 3 is a schematic structural view of the inner tube.
FIG. 4 is a diagram of the steps of the pretreatment method of the present invention.
Fig. 5 is a detailed step diagram of S2 in the present invention.
Reference numerals: inner layer pipe 1, outer layer pipe 2, filtering layer 11.
Detailed Description
Referring to fig. 1-3, the pretreatment tube for generating a tubercle bacillus liquid-based detection sample comprises an inner tube 1 and an outer tube 2, wherein the inner tube 1 is sleeved in the outer tube 2, and an interlayer for accommodating the tubercle bacillus liquid-based detection sample is formed between the outer wall of the inner tube 1 and the inner wall of the outer tube 2; the bottom of the inner layer pipe 1 is provided with a filter layer 11. When inlayer pipe 11 sets up inside outer pipe 12, outer pipe 12 is stretched out on the upper portion of inlayer pipe 11, conveniently takes out inlayer pipe like this, and the pipe diameter of the extension of inlayer pipe is greater than outer pipe, when making it add the sample that awaits measuring like this, can directly not spill and influence the testing result in outer pipe.
Further, the filter layer 11 is a hydrophobic layer having filter pores. Used for removing impurities in sputum and facilitating later observation.
Further, the diameter of the filter holes on the filter layer 11 is 10
-20
. So that the tubercle bacillus can fully enter the interlayer.
Further, the filtering layer 11 protrudes downward, and the pipe diameter of the protruding portion is gradually reduced from top to bottom. Therefore, the bottom area of the filter screen is small, the liquid attachment surface is small, the liquid can fall off more easily, and the filter screen can be filtered more fully.
Further, the filter layer 11 is in an inverted cone shape. During filtering, the liquid flows to the lowest part of the filtering layer for gathering and naturally falls under the action of gravity, so that the filtering is more sufficient.
Further, the filter layer 11 has an inverted hemispherical shape.
Furthermore, the inner bottom surface of the outer layer pipe 2 protrudes downwards, and the inner diameter of the protruding part is gradually reduced from top to bottom, so that tubercle bacillus in a liquid-based detection sample obtained after filtration is easy to deposit at the lowest part to form enrichment. The bottom of the outer layer pipe 2 is matched with the shape of the filter screen, and the bottoms of the inner layer pipes 1 are close to each other after being placed in the outer layer pipe 12, so that liquid in a to-be-detected sample added into the inner layer pipe cannot directly pass through the filter layer to cause insufficient mixing of the to-be-detected sample, and only when the inner layer pipe is separated from the outer layer pipe, substances in the outer layer pipe and the inner layer pipe can be separated to finish filtering; and when the outer bottom of the outer layer tube 2 is arranged, the diameter of the outer bottom is the same as that of the opening at the upper part, and the outer bottom surface is horizontal, so that the outer layer tube 2 can be conveniently placed.
Further, the pretreatment method is realized by adopting the pretreatment pipe, and comprises the following steps:
step S1, placing the inner tube 1 into the outer tube 2, and adding the sputum and the sputum digestive juice into the inner tube 1 for fully mixing; the sputum digestive juice separates tubercle bacillus in the sputum from the sputum.
Step S2, stirring the sputum and the sputum digestive juice in the inner tube 1 to fully mix the sputum and the sputum digestive juice, promoting rapid and full digestion of the sputum, and then taking away the inner tube 1; taking away the inner tube 1, and separating the tubercle bacillus liquid-based detection sample from the digested sputum to the interlayer through the filter layer 11 of the inner tube 1; obtaining the liquid enriched with tubercle bacillus. When the inner layer pipe is taken out, the inner layer pipe needs to be shaken or stirred, so that the inner layer pipe is fully filtered;
step S3, adding a staining agent into the outer tube 2 to stain the liquid-based detection sample. The staining is convenient for observing tubercle bacillus.
Further, the S2 further includes:
step S21, placing the pretreatment tube filled with sputum and sputum digestive juice under an ultraviolet lamp to irradiate and inactivate tubercle bacillus; the inactivated tubercle bacillus is safer and is easy to stain.
Further, the S2 further includes:
and step S22, promoting the digestion of sputum and the separation of tubercle bacillus by adopting ultrasonic oscillation. So that the tubercle bacillus in the sputum enters the liquid-based detection sample as much as possible.
And step S23, taking out the inner tube 1, and separating the tubercle bacillus liquid-based detection sample from the digested sputum to the interlayer through the filter layer 11 of the inner tube 1.
And after dyeing is finished, finishing the pretreatment process before the tubercle bacillus liquid base is used for imaging of high-definition imaging equipment, directly dripping the dyed liquid base detection sample on the high-definition imaging equipment and guiding the liquid base detection sample to an image sensor chip, and carrying out image shooting and image identification to obtain a picture with a blue background and a red tubercle bacillus. By adopting the method, the imaging is clear, the observation is convenient, the cleaning is convenient, only simple washing is needed, and the chip cannot be damaged. The liquid-based detection sample obtained by the pretreatment method disclosed by the invention is evenly distributed by carrying out centrifugal enrichment on tubercle bacillus, and each adopted liquid-based detection sample contains the tubercle bacillus without multiple collection and observation. And high-definition imaging equipment is adopted, the formed picture directly and clearly knows whether tubercle bacillus exists, and the problems that the observation effect of the existing tubercle bacillus observation method is poor and the process is complex are solved.
The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present application should be covered within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. The pretreatment tube for generating the tubercle bacillus liquid-based detection sample is characterized by comprising an inner-layer tube (1) and an outer-layer tube (2), wherein the inner-layer tube (1) is sleeved in the outer-layer tube (2), and an interlayer for accommodating the tubercle bacillus liquid-based detection sample is formed between the outer wall of the inner-layer tube (1) and the inner wall of the outer-layer tube (2); the bottom of the inner layer pipe (1) is provided with a filtering layer (11).
2. The pre-processing tube for generating a tubercle bacillus liquid based assay sample according to claim 1, wherein the filter layer (11) is a hydrophobic layer with filter pores.
3. The pre-processing tube for generating tubercle bacillus liquid based assay samples according to claim 2, wherein the filter layer (11) has filter holes with a diameter of 10 a
-20
。
4. The pre-processing tube for generating tubercle bacillus liquid based assay sample according to any of claims 1 to 3, wherein the filter layer (11) is protruded downward, and the diameter of the protruded portion is gradually reduced from top to bottom.
5. The pre-processing tube for generating tubercle bacillus liquid based assay samples according to claim 4, characterized in that the filter layer (11) is in the shape of an inverted cone.
6. The pre-processing tube for generating a tubercle bacillus liquid based assay sample according to claim 4, wherein the filter layer (11) has an inverted hemispherical shape.
7. The pre-processing tube for generating tubercle bacillus liquid based assay sample according to any of claims 1 to 3, wherein the inner bottom surface of the outer tube (2) is downwardly convex and the inner diameter of the convex portion is gradually reduced from top to bottom.
8. A pretreatment method for producing a tubercle bacillus liquid based assay sample, which is carried out using the pretreatment tube according to any one of claims 1 to 7, the pretreatment method comprising:
step S1, placing the inner tube (1) into the outer tube (2), and adding sputum and sputum digestive juice into the inner tube (1);
step S2, enabling the sputum and the sputum digestive juice to be fully mixed in the inner-layer tube (1), promoting the sputum to be rapidly and fully digested, separating the tubercle bacillus liquid-based detection sample from the digested sputum to the interlayer, and then taking away the inner-layer tube (1);
and step S3, adding a staining agent into the outer layer tube (2) to stain the liquid-based detection sample.
9. The pretreatment method for generating a tubercle bacillus liquid based assay sample according to claim 8, wherein said S2 further comprises:
and step S21, placing the pretreatment tube filled with the sputum and the sputum digestive juice under an ultraviolet lamp to irradiate and inactivate tubercle bacillus.
10. The pretreatment method for generating a tubercle bacillus liquid based assay sample according to claim 9, wherein said S2 further comprises:
step S22, promoting sputum digestion and tubercle bacillus separation by ultrasonic oscillation;
step S23, the inner tube (1) is removed, and the tubercle bacillus liquid-based detection sample is separated from the digested sputum into the sandwich layer by passing through the filter layer (11) of the inner tube (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111302965.2A CN113866096A (en) | 2021-11-05 | 2021-11-05 | Pretreatment pipe and method for generating tubercle bacillus liquid-based detection sample |
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| CN202111302965.2A CN113866096A (en) | 2021-11-05 | 2021-11-05 | Pretreatment pipe and method for generating tubercle bacillus liquid-based detection sample |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102359939A (en) * | 2011-06-08 | 2012-02-22 | 武汉兰丁医学高科技有限公司 | Method for detecting tubercle bacillus in sputamentum |
| EP3255583A1 (en) * | 2016-06-08 | 2017-12-13 | Inspace Co., Ltd. | Method of automated tuberculosis examination of a sputum smear image |
| CN108251276A (en) * | 2018-03-23 | 2018-07-06 | 成都吱吖科技有限公司 | A kind of tuberculosis infection T cell detection kit |
| CN110029142A (en) * | 2018-12-29 | 2019-07-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology |
| US20190376996A1 (en) * | 2017-02-23 | 2019-12-12 | Autobio Diagnostics Co., Ltd. | Sample adding system for automatically adding sputum digestion liquid |
| CN112574938A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Sputum treatment fluid for membrane filtration enriched bacteria and application thereof |
| CN112574885A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Mycobacterium tuberculosis detection method based on magnetic bead enrichment sputum sample and special kit thereof |
-
2021
- 2021-11-05 CN CN202111302965.2A patent/CN113866096A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102359939A (en) * | 2011-06-08 | 2012-02-22 | 武汉兰丁医学高科技有限公司 | Method for detecting tubercle bacillus in sputamentum |
| EP3255583A1 (en) * | 2016-06-08 | 2017-12-13 | Inspace Co., Ltd. | Method of automated tuberculosis examination of a sputum smear image |
| US20190376996A1 (en) * | 2017-02-23 | 2019-12-12 | Autobio Diagnostics Co., Ltd. | Sample adding system for automatically adding sputum digestion liquid |
| CN108251276A (en) * | 2018-03-23 | 2018-07-06 | 成都吱吖科技有限公司 | A kind of tuberculosis infection T cell detection kit |
| CN110029142A (en) * | 2018-12-29 | 2019-07-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology |
| CN112574938A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Sputum treatment fluid for membrane filtration enriched bacteria and application thereof |
| CN112574885A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Mycobacterium tuberculosis detection method based on magnetic bead enrichment sputum sample and special kit thereof |
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