CN1138502C - Outdoor multi-unit record system for recording electric activities of cerebral neures of rhesus monkey - Google Patents
Outdoor multi-unit record system for recording electric activities of cerebral neures of rhesus monkey Download PDFInfo
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- CN1138502C CN1138502C CNB001050397A CN00105039A CN1138502C CN 1138502 C CN1138502 C CN 1138502C CN B001050397 A CNB001050397 A CN B001050397A CN 00105039 A CN00105039 A CN 00105039A CN 1138502 C CN1138502 C CN 1138502C
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Abstract
The present invention relates to an outdoor multi-unit system for recording electric activities of the cerebral neure of a rhesus monkey, which belongs to the field of nerve electrophysiology. The system is composed of a miniature recording platform (6) and a hand held portable instrument box (11), wherein the miniature recording platform (6) is fixed at the skull surface (17) of the rhesus monkey by screw nails; the hand held portable instrument box (11) is connected with a device on the miniature recording platform (6) by a cable (5). The device on the miniature record platform (6) comprises a microelectrode miniature propeller (3), an animal behavior recording video camera (1), a miniature video eye movement recording instrument (2) and a microelectrode amplifier (4), wherein the animal behavior recording video camera, the miniature video eye movement recording instrument and the microelectrode amplifier are respectively connected with the cable (5). The hand held portable instrument box (11) connected with the cable (5) comprises a four-frequent division viewing signal synthesis instrument (7), a main amplifier (8), a processing system (9) of an eight-in-one nerve signal single chip computer, and a magnetic tape recording instrument (10) of 8mm. The system has the advantages of efficient, outdoor and field multi-unit electric discharge record of the cerebral neure of the rhesus monkey, small volume, light weight, strong interference resistance, etc.
Description
Technical field:
The present invention relates to the outdoor recording system of the many unit activities of a kind of macaque cerebral neuron, belong to the neural electrophysiology field.
Background technology:
Neuron is neural ultimate unit, is one of topmost means in the brain research to the discussion of electrical activity of neurons.The microelectrode recording of cental system neuronal cell electrical activity starts from nineteen thirty-seven, but before the seventies, this record can only carry out on anesthetized animal, has brought great restriction with regard to the research of giving brain and behavior like this.To the seventies, people have set up the technology of carrying out the unit discharge record on the clear-headed animal central nervous system who is expert at for restriction, half restriction, the foundation of this technology makes people can study under non-narcotization, the electrical activity of animal central nervous system neurons and and the relation of some behavior thereof.But because the animal health is confined, as is placed in the fixture, head, body all can not move, and make this technology in the research of brain and behavior, still have bigger restricted.After the eighties, along with the hyundai electronics science and technology development, people begin to attempt being based upon on the rodent under the free movement state, carry out the technology of neuron unit activity record.After a while, set up multiple tracks microelectrode recording technology again.
The applicant has set up the technology of carrying out neuron multiple tracks microelectrode (can reach 54 roads) record on the active primate brain of indoor freedom in 1997 first in the U.S., makes us can carry out the record of electrical activity of neurons on the active monkey of indoor freedom.But this technology still has many fatal weakness.At first, because heaviness and more technical restrictions of instrument, experiment can only be carried out indoor.The scope of animal movement can only have several square metres.Like this, when some behavioral pattern of zoologizeing, during as social behavior, this technology just seems and can not satisfy experimental requirements.
Summary of the invention:
The objective of the invention is to overcome the deficiency of prior art, a kind of volume is little, in light weight, the outdoor recording system of the many unit activities of macaque cerebral neuron of strong interference immunity and provide.
The present invention is achieved in that this system is by being screwed at miniature record platform (6) on the macaque skull surface (17) and the device on the miniature record platform (6), forming by the Portable instrument container (11) of cable (5) connection; Animal behavior record video camera (1), miniature video eye movement recording instrument (2) and microelectrode amplifier (4) that device on the miniature record platform (6) comprises microelectrode microdriver (3), is connected with cable (5) respectively; The Portable instrument container (11) that is connected with cable (5) comprises that four frequency divisions look signal synthesizer (7), main amplifier (8), eight-in-one nerve signal scm managing system (9), 8mm tape recorder (10); Connected mode in this system between each device is: animal behavior record video camera (1), miniature video eye movement recording instrument (2) are connected with four frequency division video signal synthesizers (7) respectively, and signal enters 8mm tape recorder (10) through the video input port; Signal by microelectrode output enters microelectrode amplifier (4), enters main amplifier (8) through cable (5), enters eight-in-one nerve signal scm managing system (9) again through the input port, with after audio input port enters 8mm tape recorder (10).Wherein:
The microelectrode microdriver (3) that places plastics protections boxes (12) is made up of the screw rod (11) that is connected with deposited copperplate (13) and spring (14), the pedestal (16) that is connected spring (14) and electrode insertion tube (15), the pedestal holder (20) that is fixed on the macaque skull surface (17) with rustless steel fixed mount (19) and dens supporter cement (18) that is connected with pedestal (16); The microelectrode that places electrode insertion tube (15) is by several nichrome wires stranded form of single electrode impedance at 200K-1000K ohm, the one end disperse and with the input welding of microelectrode amplifier (4), the other end inserts the macaque brain;
Microelectrode amplifier (4) and main amplifier (8) are welded by delivery circuit that places box and operational amplifier respectively, and its main electricity index is: input impedance: 10
12Ohm, static noise level:＜0.1 microvolt; Microelectrode gain amplifier: 100: main amplifier gain: 30; Working band: 900-3000HZ; Power supply: two 9V;
Scenery record video camera (1), miniature video eye movement recording instrument (2), four frequency division video signal synthesizers (7), eight-in-one nerve signal scm managing system (9), 8mm tape recorder (10) are known equipment in the animal visual field in this device.
System work process is: after the neuron signal of telecommunication is picked up by microelectrode, send into microelectrode amplifier (4) and amplify, send into then in the eight-in-one nerve signal scm managing system (9) and handle, the signal after the processing exists on the sound channel of 8mm tape recorder (10).
The signal of animal behavior on the miniature record platform (6) record video camera (1) and miniature video eye movement recording instrument (2) is sent into four frequency divisions in the Portable instrument container (11) through cable (5) and is looked the signal that signal synthesizer (7), this instrument handled and exist on the video channel of 8mm tape recorder (10).
The present invention has following advantage:
1. the tradition that electric physiology can only carry out in electric Physiological Experiment chamber has been broken in the use of this covering device, makes the electrophysiological research of neuron no longer be confined to the laboratory behavior.And realized first that in the world primate carries out many unit discharges of neuron record in outdoor, field;
2. adopt this covering device, make us can write down the electrical activity of neurons in a plurality of brains district simultaneously, this is very important to the mutual function of studying each brain district.Simultaneously, can extract signal efficiently, make us can gather lot of data at short notice, this is to studying a lot of nerves, mental sickness beyond doubt of great use;
3. little, in light weight, the strong interference immunity of this covering device system bulk.
Description of drawings:
Accompanying drawing 1 is native system structure assembling sketch map.
Accompanying drawing 2 is the connected mode sketch map between each device in the native system.
Accompanying drawing 3 is the structural representation of microelectrode microdriver (3).
Accompanying drawing 4 is the circuit diagram of microelectrode amplifier (4) and main amplifier (8).
Accompanying drawing 5 is four unification microelectrode sketch maps.
The specific embodiment:
The invention will be further described below in conjunction with accompanying drawing:
This system is by being screwed at miniature record platform (6) on the macaque skull surface (17) and the device on the miniature record platform (6), forming by the Portable instrument container (11) of cable (5) connection; Animal behavior record video camera (1), miniature video eye movement recording instrument (2) and microelectrode amplifier (4) that device on the miniature record platform (6) comprises microelectrode microdriver (3), is connected with cable (5) respectively; The Portable instrument container (11) that is connected with cable (5) comprises that four frequency divisions look signal synthesizer (7), main amplifier (8), eight-in-one nerve signal scm managing system (9), 8mm tape recorder (10); Connected mode in this system between each device is: animal behavior record video camera (1), miniature video eye movement recording instrument (2) are connected with four frequency division video signal synthesizers (7) respectively, and signal enters 8mm tape recorder (10) through the video input port; Signal by microelectrode microdriver (3) output enters microelectrode amplifier (4), enter main amplifier (8) through cable (5), enter eight-in-one nerve signal scm managing system (9) again through the input port, with after audio input port enters 8mm tape recorder (10).Wherein:
The microelectrode microdriver (3) that places plastics protections boxes (12) is made up of the screw rod (11) that is connected with deposited copperplate (13) and spring (14), the pedestal (16) that is connected spring (14) and electrode insertion tube (15), the pedestal holder (20) that is fixed on the macaque skull surface (17) with rustless steel fixed mount (19) and dens supporter cement (18) that is connected with pedestal (16); Its parameter is as follows: minimum advance distance: 40 microns; Per 360 degree advance distances: 330 microns; Maximum advance distance: 8 millimeters; Weight: less than 1 gram.The microelectrode that places electrode insertion tube (15) by single electrode impedance at 200K-1000K ohm, test frequency is that the 2-4 root diameter of 1000HZ is 0.015 millimeter, stranded the forming of the insulating nichrome wire of Te Fulong (U.S. California Fine wire Comp provides), the length of microelectrode is decided on the brain structure of record, the one end disperse and with the input welding of microelectrode amplifier (4), the other end inserts the macaque brain.
Microelectrode amplifier (4) and main amplifier (8) are welded by delivery circuit that places box and operational amplifier respectively, and its main electricity index is: input impedance: 10
12Ohm, static noise level:＜0.1 microvolt; Microelectrode gain amplifier: 100: main amplifier gain: 30; Working band: 900-3000HZ; Power supply: two 9V; Volume: 50 millimeters * 40 millimeters * 10 millimeters; Weight: 10 grams.
Scenery record video camera (1), miniature video eye movement recording instrument (2), four frequency division video signal synthesizers (7), eight-in-one nerve signal scm managing system (9), 8mm tape recorder (10) are known equipment in the animal visual field in this device.
The function of this system is divided into electricity physiological signal collection, processing, recording section and animal eye movement, behavioral data collection, processing, recording section.Electricity physiological signal collection, processing, recording section are made up of microelectrode microdriver (3), microelectrode, microelectrode amplifier (4), main amplifier (8), eight-in-one nerve signal scm managing system (9) and 8mm tape recorder (10).Animal eye movement, behavioral data collection, processing, recording section write down video camera (1), miniature video eye movement recording instrument (2), four frequency division video signal synthesizers (7) and 8mm tape recorder (10) by animal behavior and form.
System work process is: after the neuron signal of telecommunication is picked up by microelectrode, send into microelectrode amplifier (4) and amplify, send into then in the eight-in-one nerve signal scm managing system (9) and handle, the signal after the processing exists on the sound channel of 8mm tape recorder (10).
The signal of animal behavior on the miniature record platform (6) record video camera (1) and miniature video eye movement recording instrument (2) is sent into four frequency divisions in the Portable instrument container (11) through cable (5) and is looked the signal that signal synthesizer (7), this instrument handled and exist on the video channel of 8mm tape recorder (10).
Miniature video eye movement recording instrument (2) is made up of minisize pick-up head (U.S. MARSHALL COMP, model V-X0071) and corresponding conventional mechanical fixture.Major parameter is as follows: working environment illumination:＜10LUX; Sample frequency: 25 times/second; Eye-movement measurement precision:＜1 degree.Before the eye movement instrument is installed in animal eye, adjust instrument angle and with the distance of eyes, make eyeball just in time be positioned at the central authorities in the instrument visual field.The eye movement signal is delivered in the four split screen video signal synthesizers.
Animal behavior record video camera (1) is selected (U.S. MARSHALL COMP, model V-X0071), and the major function of this instrument is the behavior of record animal, and the behavior video of animal is preserved.
Four frequency divisions are looked signal synthesizer (7) and are selected (U.S. GOLDBEAM COMP, model QB-BNC) for use, utilize this equipment, can be in the open air with under the outdoor conditions the four road video signals and real-time the synthesizing of behavioral data of separate sources being stored.
Eight-in-one nerve signal scm managing system (9) is selected (Qidong computer technology factory production for use, model is MC-5160) computer, the signal handler that adopts the inventor to provide, to form standard TTL signal, again through Integral Processing from the signal process shaping of 8 microelectrodes, the voice modulation coding, every 100MS deposits the neuron discharge signal in the magnetic tape controller in as a time memory cell, for processed offline.
8mm tape recorder (10) select video tape recording instrument (model is the E-660 of Sharp) for use, the signal of record is the N standard signal, all behaviors, electric physiological data all are recorded in this instrument, for computer analysis.
Before the record, elder generation under deep anaesthesia, is fixed in animal on the brain solid positioner, cuts skin, behind the exposure skull, carefully cleans skull surface with hydrogen peroxide.Carefully proofread and correct the position of miniature instrument platform and on skull, carry out labelling.In mark that the set bolt of platform is bonding with skull with dental base acrylic resin powder.Boring a diameter at the record position of brain is 0.5 millimeter aperture, and the angle of rake pedestal of microelectrode is fixed on the aperture top.At all the other position screw-in lengths of brain 3 millimeters rustless steel set bolt 8-10.Expose portion with skull covers with dental base acrylic resin powder then.The microelectrode propeller is fixed on the pedestal.In one week of postoperative, begin formal experiment.Earlier animal is placed fixedly chair of monkey, miniature record platform is fixed on the head, the input of preamplifier is gone between with microelectrode to link to each other.Power-on, initialization whole system, slowly turn microelectrode propeller.Separate the neuron that records.After finding neuron, carefully adjust eight-in-one nerve signal scm managing system again, make the TTL signal of a specific customizing messages passage of neuron pulse-triggered.All data all adjust finish after, animal is discharged from fixing chair, take the experiment place to and experimentize.
With amplifier, deliver in the portable instrument container of experimenter by the output signal of microcam and eye movement monitor with 1.5 meters long cables for collection of experiment data.
Claims (2)
1. the outdoor recording system of the many unit activities of macaque cerebral neuron is characterized in that this system is by being screwed at miniature record platform (6) on the macaque skull surface (17) and the device on the miniature record platform (6), forming by the Portable instrument container (11) of cable (5) connection; Animal behavior record video camera (1), miniature video eye movement recording instrument (2) and microelectrode amplifier (4) that device on the miniature record platform (6) comprises microelectrode microdriver (3), is connected with cable (5) respectively; The Portable instrument container (11) that is connected with cable (5) comprises that four frequency divisions look signal synthesizer (7), main amplifier (8), eight-in-one nerve signal scm managing system (9), 8mm tape recorder (10); Connected mode in this system between each device is: animal behavior record video camera (1), miniature video eye movement recording instrument (2) are connected with four frequency division video signal synthesizers (7) respectively, and signal enters 8mm tape recorder (10) through the video input port; Signal by microelectrode microdriver (3) output enters microelectrode amplifier (4), enter main amplifier (8) through cable (5), enter eight-in-one nerve signal scm managing system (9) again through the input port, with after audio input port enters 8mm tape recorder (10).
2. by the outdoor recording system of the claim 1 many unit activities of described macaque cerebral neuron, it is characterized in that:
2.1 the microelectrode microdriver (3) that places plastics protections boxes (12) is made up of the screw rod (11) that is connected with copper clad plate (13) and spring (14), the pedestal (16) that is connected spring (14) and electrode insertion tube (15), the pedestal holder (20) that is fixed on the macaque skull surface (17) with rustless steel fixed mount (19) and dens supporter cement (18) that is connected with pedestal (16); The microelectrode that places electrode insertion tube (15) is by several nichrome wires stranded form of single electrode impedance at 200K-1000K ohm, the one end disperse and with the input welding of microelectrode amplifier (4), the other end inserts the macaque brain;
2.2 microelectrode amplifier (4) and main amplifier (8) are welded by delivery circuit that places box and operational amplifier respectively, its main electricity index is: input impedance: 10
12Ohm, static noise level:＜0.1 microvolt; Microelectrode gain amplifier: 100: main amplifier gain: 30; Working band: 900-3000HZ; Power supply: two 9V;
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| Application Number | Priority Date | Filing Date | Title |
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| CNB001050397A CN1138502C (en) | 2000-04-08 | 2000-04-08 | Outdoor multi-unit record system for recording electric activities of cerebral neures of rhesus monkey |
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| CNB001050397A CN1138502C (en) | 2000-04-08 | 2000-04-08 | Outdoor multi-unit record system for recording electric activities of cerebral neures of rhesus monkey |
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| CN1138502C true CN1138502C (en) | 2004-02-18 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100455015C (en) * | 2006-03-09 | 2009-01-21 | 西安交通大学 | Integrated vision monitoring multi-mode wireless computer interactive apparatus |
| CN101803913B (en) * | 2010-04-08 | 2011-11-23 | 山东科技大学 | Neuroelectric activity recording device and method for animal behaviors in natural environment |
| EP2720611B1 (en) * | 2011-06-20 | 2016-04-20 | The Regents of The University of California | Neuron recording system |
| US8706662B2 (en) * | 2011-07-21 | 2014-04-22 | Qualcomm Incorporated | Method and apparatus of controlling noise associated with synaptic inputs based on neuronal firing rate |
| CN107149472A (en) * | 2017-06-26 | 2017-09-12 | 北京大学 | Suitable for many brain area information gathering electrode propellers of rat |
| CN110037679A (en) * | 2018-01-15 | 2019-07-23 | 王�琦 | Multi-channel adjustable electrode suitable for rat |
| CN112345616B (en) * | 2020-09-14 | 2022-03-11 | 中国科学院生物物理研究所 | High-precision electrochemical detection method suitable for detecting biological active substances at single cell and sub-cell level |
| CN112595842A (en) * | 2020-11-26 | 2021-04-02 | 中国科学院深圳先进技术研究院 | Detection device and detection method for in-situ cell single vesicle inclusion |
| CN114224358A (en) * | 2021-12-15 | 2022-03-25 | 中国科学院深圳先进技术研究院 | Recording device |
| CN116369951A (en) * | 2023-04-18 | 2023-07-04 | 中国科学院昆明动物研究所 | Method for in-vivo wireless recording of cells at sea horse positions of tree shrew |
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