CN113832191A - Lentiviral vectors, systems and uses thereof - Google Patents

Abstract

The invention discloses a lentiviral vector which has a truncated cis-acting element, wherein the sequence of the truncated cis-acting element is shown as SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6 or SEQ ID NO. 8. The invention also discloses a lentiviral vector system based on the lentiviral vector, a host cell, an application and a transfection method.

Description

Lentiviral vectors, systems and uses thereof

Technical Field

The invention relates to the technical field of biological medical treatment, in particular to a lentiviral vector, a lentiviral system and application of the lentiviral vector.

Background

Lentiviral vectors are viral vectors developed based on HIV-1 (human immunodeficiency virus type I). Lentivirus vectors can efficiently infect various types of cells such as neuronal cells, liver cells, cardiac muscle cells, tumor cells, endothelial cells, stem cells, and the like, and in addition, have an infection ability to both split cells and non-split cells, unlike retrovirus vectors derived from mouse leukemia virus. The vector can effectively integrate the exogenous gene onto the host chromosome, thereby achieving persistent expression; meanwhile, the lentivirus vector is different from retrovirus which may cause tumors due to integration, and the safety of the lentivirus vector is proved by various clinical researches at present, so that the lentivirus vector has a wide development prospect.

Lentiviruses belong to the family of retroviruses, but have a complex genome structure, comprising 4 accessory genes, vif, vpr, nef, vpu and 2 regulatory genes tat and rev, in addition to 3 structural genes, gag, pol and env, which are similar to those of a simple retrovirus. HIV-1 is the most characteristic virus of lentiviruses, and the first lentivirus vector system is constructed based on this virus. Lentiviral vectors are constructed by separating cis-acting elements (e.g., packaging signals, long terminal repeats) from sequences encoding trans-acting proteins in the HIV-1 genome. The carrier system comprises a packaging component and a carrier component: the packaging component is constructed from the HIV-1 genome with the cis-acting sequences required for packaging, reverse transcription and integration removed, and is capable of supplying in trans the proteins required for the production of viral particles; the vector component is complementary to the packaging component, contains HIV-1 cis-acting sequences required for packaging, reverse transcription and integration, and has a multiple cloning site under the control of a heterologous promoter and a target gene inserted at the site. In order to reduce the possibility of producing Replication Competent Virus (RCV) by homologous recombination of the two components, the third generation vector obtained RSV promoter by replacing 5 'LTR of the packaging component with Cytomegalovirus (CMV) immediate early promoter, deletion mutation of 3' LTR, addition of SV40 polyA site, and the like. The packaging components were constructed on two plasmids, one expressing gag and pol and the other expressing env, respectively. Based on this principle, a three-plasmid expression system was constructed. The three-plasmid expression system comprises a packaging plasmid, an envelope protein plasmid and a shuttle plasmid. Wherein the packaging plasmid expresses all trans-activation proteins required for HIV-1 replication under the control of a CMV promoter, but does not produce viral envelope proteins and accessory proteins vpu; envelope protein plasmid codes vesicular stomatitis virus G protein (VSV-G), and the pseudoconfigurational lentiviral vector enveloped by the VSV-G expands the tropism range of target cells of the vector, increases the stability of the vector, allows the vector to be concentrated by high-speed centrifugation, and improves the titer; the shuttle plasmid contains the sequences of RRE, cPPT/CTS and the like (see FIG. 1) in addition to cis-sequences required for packaging, reverse transcription and integration, and a target gene (GOI, gene of interest) is inserted therein.

In any lentiviral vector, cis-acting elements such as the 5' R, U5, Packaging Signal (PSI), RRE and cPPT/CTS, which are required for packaging the lentiviral genome into viral particles, must be retained. At present, the length of the partial sequence is about 1720bp on a general lentivirus vector, wherein the partial sequence comprises 199bp of a 5' end LTR (R + U5 region) sequence, 491bp of a PSI sequence, 858bp of an RRE sequence, 147bp of a cPPT/CTS sequence and 25bp of other sequences (mainly enzyme cutting sites) for connection.

The efficiency of lentiviral vector packaging for virus is inversely related to the length of the foreign gene inserted, with longer sequences resulting in lower lentiviral titers. For example, spCas9 most commonly used in scientific research belongs to a large gene, the length of the gene is more than 4.2kb, the use of a lentiviral vector for the gene is limited by the capacity of the lentiviral vector, the length of the gene is further lengthened after fusion with EGFP fluorescence and other labels, and the virus-releasing efficiency is seriously influenced. For this reason, it is important to increase the capacity of lentiviral vectors.

Disclosure of Invention

In view of the above, it is necessary to provide a lentiviral vector, a system and applications thereof, aiming at the problem of small capacity of the lentiviral vector.

The first purpose of the invention is to provide a lentivirus vector which has a truncated cis-acting element, and the sequence of the truncated cis-acting element is shown as SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6 or SEQ ID NO. 8.

Optionally, the truncated cis-acting element is linked at its 3' end to a gene segment of interest.

In some embodiments, the target gene fragment has a length ranging from 4500bp to 7700 bp.

In some embodiments, the gene fragment of interest is selected from at least one of spCas9, dspsca 9, ascipf 1, and LbCpf 1.

It is a second object of the present invention to provide a lentiviral vector system comprising a packaging component and the lentiviral vector.

Optionally, the lentiviral vector system is a two-plasmid, three-plasmid or four-plasmid system.

The third purpose of the invention is to provide a slow virus particle obtained by packaging the slow virus vector system.

The fourth purpose of the invention is to provide the application of the lentiviral vector system in the preparation of drugs for gene therapy.

It is a fifth object of the invention to provide a host cell which is infected and transduced with said lentiviral vector system.

The sixth purpose of the invention is to provide a lentivirus transfection method, and the system containing the lentivirus vector is transferred into a host cell.

According to the invention, the specific cis-element sequence of the lentiviral vector is truncated, a novel lentiviral vector capable of effectively carrying a long sequence is screened, compared with a wild lentiviral vector, the truncated lentiviral vector has small influence on the virus-out efficiency, but the expansion capacity is increased, and the infection capacity is increased after the virus is packaged.

Drawings

FIG. 1 is a schematic diagram of a shuttle plasmid structure of a conventional lentiviral vector;

FIG. 2 shows the expression cassette pSLenti-CMV-EGFP-P2A-Luciferase-PGK-Puro-WPRE according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of the construction of a lentiviral vector truncation in accordance with one embodiment of the present invention;

FIG. 4 is a fluorescent photograph of a 293T cell infected with a truncated viral packaging supernatant according to an embodiment of the present invention;

FIG. 5 shows the Luciferase ratio results for truncates versus WT according to one embodiment of the present invention;

FIG. 6 is a fluorescent photograph of D12 truncation infecting 293T at the same MOI (MOI =10) as WT virus according to an embodiment of the present invention;

FIG. 7 shows the ratio of Luciferase in 293T infection with the D12 truncation at the same MOI (MOI =10) as the WT virus according to an embodiment of the present invention;

FIG. 8A is a map of a WT-spCas9-P2A-EGFP lentiviral vector, in accordance with one embodiment of the invention;

FIG. 8B is a D12-spCas9-P2A-EGFP lentiviral vector map of one embodiment of the invention;

FIG. 9 is a fluorescent photograph of infection of 293T with WT-spCas9-P2A-EGFP and D12-spCas9-P2A-EGFP at the same MOI (MOI =10) according to an embodiment of the invention;

FIG. 10 is a gray scale analysis of WT-spCas9-P2A-EGFP and D12-spCas9-P2A-EGFP expression levels according to an embodiment of the present invention;

FIG. 11A is a pSLenti-CMV-SDK 2-3xFlag-WPRE expression vector according to an embodiment of the present invention;

FIG. 11B shows the pSLenti-D12-CMV-SDK 2-3xFlag-WPRE expression vector according to an embodiment of the present invention;

FIG. 12 is a graph showing the overexpression detection of pSLenti-CMV-SDK 2-3xFlag-WPRE and pSLenti-D12-CMV-SDK 2-3xFlag-WPRE in accordance with one embodiment of the present invention;

FIG. 13 is a fluorescent photograph of D08 truncation infecting 293T at the same MOI (MOI =10) as WT virus according to an embodiment of the present invention;

FIG. 14 shows the ratio of Luciferase in 293T infection with the D08 truncation at the same MOI (MOI =10) as the WT virus according to one embodiment of the present invention;

FIG. 15A is a map of a WT-spCas9-P2A-EGFP lentiviral vector, in accordance with one embodiment of the invention;

FIG. 15B is a D08-spCas9-P2A-EGFP lentiviral vector map of one embodiment of the invention;

FIG. 16 is a fluorescent photograph of infection of 293T with WT-spCas9-P2A-EGFP and D08-spCas9-P2A-EGFP at the same MOI (MOI =10) according to an embodiment of the invention;

FIG. 17 is a gray scale analysis of WT-spCas9-P2A-EGFP and D08-spCas9-P2A-EGFP expression levels according to an embodiment of the present invention;

FIG. 18A is a pSLenti-CMV-SDK 2-3xFlag-WPRE expression vector according to an embodiment of the present invention;

FIG. 18B shows a pSLenti-D08-CMV-SDK 2-3xFlag-WPRE expression vector according to an embodiment of the present invention;

FIG. 19 is a graph showing the overexpression detection of pSLenti-CMV-SDK 2-3xFlag-WPRE and pSLenti-D08-CMV-SDK 2-3xFlag-WPRE in accordance with one embodiment of the present invention.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In the first aspect, the invention truncates the cis-acting element sequence consisting of a promoter region, R region, U5, PSI, RRE and cPPT/CTS which are sequentially connected from 5 'end to 3' end, and screens to obtain a truncation body which does not influence the virus-removing efficiency and infection expression capability of the original lentiviral vector, so that the insertable target gene segment length of the lentiviral vector is expanded.

A "promoter" is a DNA sequence that directs the binding of RNA polymerase and thereby initiates RNA synthesis. The promoters used in the present invention allow expression in a wide variety of cell and tissue types; or may be a cell-free specific promoter, or may be a "cell-specific", "cell type-specific", "cell lineage-specific" or "tissue-specific" promoter, which allows expression in a limited variety of cell and tissue types, respectively. In particular embodiments, it may be desirable to use cell, cell type, cell lineage, or tissue specific expression control sequences to achieve cell type specific, cell lineage specific, or tissue specific expression of a desired polynucleotide sequence (e.g., to express a nucleic acid encoding a polypeptide only in a subpopulation of cell types, cell lineages, or tissues, or at a particular developmental stage).

Illustrative examples of tissue-specific promoters include, but are not limited to: b29 promoter (B cell expression), run transcription factor (CBFa2) promoter (stem cell specific expression), CD14 promoter (monocyte expression), CD43 promoter (leukocyte and platelet expression), CD45 promoter (hematopoietic cell expression), CD68 promoter (macrophage expression), CYP4503a4 or ALB promoter (hepatocyte expression), myodesmin promoter (muscle cell expression), elastase I promoter (pancreatic acinar cell expression), endoglin promoter (endothelial cell expression), fibroblast specific protein I promoter (FSPl) promoter (fibroblast expression), fibronectin promoter (fibroblast expression), fms-related tyrosine kinase I fltl promoter (endothelial cell expression), Glial Fibrillary Acidic Protein (GFAP) promoter (astrocyte expression), insulin promoter (pancreatic cell expression), Integrin-alpha-2 b (ITGA2B) promoter (megakaryocytes), intracellular adhesion molecule 2(ICAM-2) promoter (endothelial cells), interferon-beta (IFN-beta) promoter (hematopoietic cells), keratin 5 promoter (keratinocyte expression), Myoglobin (MB) promoter (muscle cell expression), myogenic differentiation I (MYOD1) promoter (muscle cell expression), nephroprotein promoter (podocyte expression), bone gamma-carboxyglutamic acid protein 2(OG-2) promoter (osteoblast expression), 3-keto acid CoA transferase 2B (Oxct2B) promoter (haploid sperm cell expression), surface-activated protein B (SP-B) promoter (lung cell expression), synaptoprotein promoter (nerve cell expression), Wiskott-Aldrich syndrome protein (WASP) promoter (hematopoietic cell expression).

In some embodiments, the promoter is a cell-free specific promoter. Exemplary cell-free specific promoters include, but are not limited to, Cytomegalovirus (CMV) very early promoter, viral simian virus 40 (SV40) (e.g., early or late), moloney murine leukemia virus (MoMLV) LTR promoter, Rous Sarcoma Virus (RSV) LTR, Herpes Simplex Virus (HSV) (thymidine kinase) promoter, H5, P7.5, and Pll promoter of vaccinia virus, elongation factor l- α (EFla) promoter, early growth response i (egrl), ferritin H (ferh), ferritin l (ferl), 3_ glyceraldehyde phosphate dehydrogenase (GAPDH), eukaryotic translation initiation factor 4A1 (EIF4A1), heat shock 70kDa protein 5 (HSPA5), heat shock protein 90- β member 1(HSP90B1), heat shock protein 70kDa (HSP70), β -kinesin (β -KIN), human R0SA26 locus (irs et al, ion et al, seq. (2007) Nature Biotechnology25, 1477-1482), ubiquitin C promoter (UBC), phosphoglycerate kinase-1 (PGK) promoter, cytomegalovirus enhancer/chicken β -actin (CAG) promoter, and β -actin promoter.

In some preferred embodiments, the promoter is a strong promoter.

In some preferred embodiments, the promoter is CMV, EF1a, SFH, CAG, CBh, UBC, SFFV, SV40, RSV, mCMV, GAPDH, PGK, CASI, SMVP, GUSB (hGBp), or UCOE.

In the examples of the present invention, the truncation is performed based on the cis-acting element shown in SEQ ID NO. 1, i.e., the truncated cis-acting element is a nucleic acid fragment having the deletion at the position indicated in the cis-acting element shown in SEQ ID NO. 1. PSI and RRE were used as truncation subjects, and 14 truncations were selected for study and detection. The composition of the cis-acting element shown in SEQ ID NO. 1 is as follows: r region (1-96) -U5 (97-181) -PSI (182-.

The embodiment of the invention provides a lentivirus vector, which has a truncated cis-acting element, wherein the truncated cis-acting element is based on the cis-acting element shown in SEQ ID NO. 1 and has deletions at one or more of the following positions:

1) any one or more bases from position 699 to position 745;

2) any one or more bases from position 1144 to position 1331;

3) any one or more bases from position 1486 to position 1559.

The A-th to B-th bits of the present invention include the endpoints A and B. For example, deletion of any one or more bases from position 699 to position 745 refers to deletion of all bases at either position 699 or 745 or any one, two, more or both endpoints thereof. The X-th site refers to the X-th site from the 5 '-end R region of the reference fragment gene (R-PSI-RRE-cPPT/CTS) to the 3' -end of the cPPT/CTS. For example, position 699 refers to position 699 from the 5 'end to the 3' end of the reference fragment gene (R-PSI-RRE-cPPT/CTS).

Alternatively, the missing bases are bases that are arranged consecutively or bases that are not arranged consecutively.

Alternatively, 1) all sequences from position 699 to 745 th are deleted.

Alternatively, 2) the entire sequence from position 1144 to position 1331 is deleted.

Alternatively, 3) all sequences from position 1486 to position 1559 are deleted.

In some embodiments, the truncated cis-acting element sequence is as set forth in SEQ ID NO 2 (D01), SEQ ID NO 3 (D03), SEQ ID NO 4 (D04), SEQ ID NO 5 (D05), SEQ ID NO 6 (D06), or SEQ ID NO 8 (D08).

Optionally, the 3 'end of the cPPT/CTS, i.e., the 3' end of the truncated cis-acting element, is ligated to a gene segment of interest.

In some embodiments, the length of the target gene fragment ranges from 4500bp to 7700 bp.

In some embodiments, the gene fragment of interest is selected from at least one of spCas9, dspsca 9, ascipf 1, and LbCpf 1.

In a second aspect, embodiments of the present invention provide a lentiviral vector system comprising a packaging component and the lentiviral vector.

Optionally, the lentiviral vector system is a two-plasmid, three-plasmid or four-plasmid system.

In a third aspect, the embodiments of the present invention provide a lentiviral particle obtained by packaging the lentiviral vector system.

In a fourth aspect, the embodiments of the present invention provide a use of the lentiviral vector system in the preparation of a medicament for gene therapy.

In a fifth aspect, embodiments of the invention provide a host cell infected and transduced with the lentiviral vector system.

In some embodiments, the host cell is a mammalian cell or an avian animal cell.

In some embodiments, the host cell may also be a fish cell or an amphibian cell.

In some embodiments, the host cell is a rodent cell, e.g., rat, mouse, hamster.

In some embodiments, the host cell is a primate cell, preferably a human.

In some embodiments, the host cell is a primary cell, e.g., a tumor cell, a liver cell, a cardiac muscle cell, a neuron, an endothelial cell, a stem cell, and the like.

In some embodiments, the host cell is a cell line;

common cell lines are for example:

human-derived cell lines:

293T、IMR-90、W1-38、A549、A431、BHL-100、BeWo、Caco-2、Chang、HCT-15、HeLa、HEp-G2、HEp-2、HT-1080、HT-29、JEG-2、MCF7、KB、Saos-2、WI-38、WISH、WS1、HUVEC、EB-3、Raji、IM-9、Daudi、H9、HL-60、Jurkat、K-562、U937、KG-1；

mouse-derived cell lines:

McCoy、BALB/3T3、3T6、A9、AtT-20、Clone M-3、I-10、Y-1、WEHI-3b、ES-D3、F9；

hamster-derived cell lines:

BHK-21、HaK、CHO-K1；

rat-derived cell lines:

AR42J、BRL3A、Clone 9、H4--Ⅱ-E-C3、GH1、GH3、IEC-6、L2、XC、LLC-WRC 256、Jensen、Rat2(TK-)、PC12、L6；

cell lines from other animals:

D-17、BT、MARC-145、CV-1、COS-1、COS-3、COS-7、Vero、B95-8、CRFK。

in a sixth aspect, the embodiments of the present invention provide a method for lentivirus transfection, wherein a system comprising the lentivirus vector is transferred into a host cell.

Embodiments of the present invention will be described in detail with reference to examples.

The following are specific examples.

Example 1 truncation construction and comparison of virulence

The truncation body modification is carried out on the basis of a pSLenti-CMV-EGFP-P2A-Luciferase-PGK-Puro-WPRE virus vector. The expression cassette of the pSLenti-CMV-EGFP-P2A-Luciferase-PGK-Puro-WPRE wild-type viral vector is shown in FIG. 2. The wild-type cis-acting element sequence segment of the wild-type viral vector SEQ ID NO. 1 is taken as a reference to carry out the truncation, and 14 cis-acting element sequence truncations are constructed and named as D01-D14, wherein the construction schematic diagram of the truncations of D01 and D03-D14 is shown in figure 3. By testing the ability of these truncates to detoxify and infect, capacity-expanded truncates were obtained. The nucleotide sequences of D01 and D03-D14 are respectively shown as SEQ ID NO 2-14 in sequence.

Viral infection and luciferase detection:

after virus packaging under the same conditions, 100ul of the supernatant was used to infect 293T, and a fluorescent photograph was taken at 72 hr. The results are shown in FIG. 4.

And simultaneously detecting the luciferase in the cell sample. The results are shown in FIG. 5.

The results of FIGS. 4-5 show that the truncated forms D01, D03-D06, D08, and D10-D12 still have high toxin-removing efficiency. Although the length of the truncated D01 and D03-D06 is not large, the length of the vector itself is shortened compared with that of the wild type. The detoxification efficiency of D08 was hardly affected significantly (>91%) when the length of the vector itself was reduced by 309bp compared to the wild type. D12 still maintained a relatively high detoxification efficiency (>72%) within the maximum range that could be truncated (459 bp). The truncation of the truncation body D07 on the RRE exceeds the range from 1144 th bit to 1331 th bit, the truncation of the truncation body D09 on the RRE exceeds the range from 1486 th bit to 1559 th bit, and the truncations of the truncation bodies D13 (491 th bit to 690 th bit) and D14 (200 th bit to 249 th bit) on the PSI exceed the range of D12, so that the toxic effect is seriously influenced.

Example 2.

To verify the infectivity and expression ability of the D12 virus particles, infection experiments were performed at the same multiplicity of infection (MOI). As shown in FIG. 6, the D12 truncation was more infectious than the Wild Type (WT) 72 hours after 293T infection at the same MOI.

As shown in FIG. 7, the gene expression ability of the truncated form D12 was increased by about 2-fold compared with that of the wild type under the same multiplicity of infection.

Example 3 expression of spCas9-P2A-EGFP Gene

To verify the carrying capacity of the D12 truncation on the oversized gene, wild-type and truncation spCas9 overexpression vectors were constructed. pSLenti-U6-gRNA v2.0-CMV-spCas9-P2A-EGFP-WPRE (WT-spCas 9-P2A-EGFP) and pSLenti-D12-U6-gRNA v2.0-CMV-spCas9-P2A-EGFP-WPRE (D12-spCas 9-P2A-EGFP). The total length of U6-gRNA-CMV-spCas9-P2A-EGFP-WPRE in the two vectors is 6.6 kb. The lentivirus vector maps are shown in FIG. 8A and FIG. 8B, respectively.

Lentiviral packaging was performed under the same conditions and resuspended in the same volume of PBS to a D12-spCas-P2A-EGFP (2.3 x 10 TU/ml) titer that was more than 3-fold that of WT-spCas9-P2A-EGFP (7.2 x 10 TU 7 ml).

Fluorescence photographs of infection at the same MOI with 293T are shown in FIG. 9.

According to gray scale analysis, the expression level of D12-spCas9-P2A-EGFP is about 5 times or more of that of WT-spCas9-P2A-EGFP, as shown in FIG. 10.

Example 4.6.5 kb protein encoding Gene overexpression

In order to further verify the carrying capacity of the truncated D12 to other large genes, a human SDK2 gene (NM-001144952) is selected, the length is 6519bp, the length is 6594bp after a 3xFlag label is fused at the C end, a WT lentiviral vector (pSLenti-CMV-SDK 2-3xFlag-WPRE) is packaged, and the virus titer after purification cannot reach the power of 7 due to serious overload. The SDK2 gene was constructed into a D12 truncation to obtain pSLenti-D12-CMV-SDK 2-3xFlag-WPRE (FIGS. 11A and 11B), the lentivirus packaging gave a titer of 1.2 x 10^8 TU/ml, and expression of SDK2-3xFlag was detected by Western Blot using Flag antibody (FIG. 12). From the data, the D12 truncated body can be judged to have certain bearing capacity on the large gene, the virus output is increased under the same condition, and the target gene expression can be detected.

Example 5.

To verify the infectivity and expression ability of the D08 virus particles, infection experiments were performed at the same multiplicity of infection (MOI). As shown in FIG. 13, the D08 truncation was more infectious than WT 72 hours after 293T infection at the same MOI.

As shown in FIG. 14, the gene expression ability of the truncated form D08 was increased by about 1.3 times compared with that of the wild type under the same multiplicity of infection.

Example 6 expression of spCas9-P2A-EGFP Gene

To verify the carrying capacity of the D08 truncation on the oversized gene, wild-type and truncation spCas9 overexpression vectors were constructed. pSLenti-U6-gRNA v2.0-CMV-spCas9-P2A-EGFP-WPRE (WT-spCas 9-P2A-EGFP) and pSLenti-D08-U6-gRNA v2.0-CMV-spCas9-P2A-EGFP-WPRE (D08-spCas 9-P2A-EGFP). The total length of U6-gRNA-CMV-spCas9-P2A-EGFP-WPRE in the two vectors is 6.6 kb. The lentivirus vector maps are shown in FIG. 15A and FIG. 15B, respectively.

Lentiviral packaging was performed under the same conditions and resuspended in the same volume of PBS to a D08-spCas-P2A-EGFP (1.2 x 10^8 TU/ml) titer that was more than 3 times that of the WT-spCas9-P2A-EGFP (7.2 x 10^7 TU/ml).

Fluorescence photographs of 293T infected at the same MOI are shown in FIG. 16.

According to gray scale analysis, the expression level of D08-spCas9-P2A-EGFP is about 2.5 times or more than that of WT-spCas9-P2A-EGFP, as shown in FIG. 17.

Example 7.6.5 kb encoding protein Gene overexpression

In order to further verify the carrying capacity of the truncated D08 to other large genes, a human SDK2 gene (NM-001144952) is selected, the length is 6519bp, the length is 6594bp after a 3xFlag label is fused at the C end, a WT lentiviral vector (pSLenti-CMV-SDK 2-3xFlag-WPRE) is packaged, and the virus titer after purification cannot reach the power of 7 due to serious overload. The SDK2 gene was constructed into a D08 truncation to yield pSLenti-D08-CMV-SDK 2-3xFlag-WPRE (FIGS. 18A and 18B). Lentiviral packaging gave titers of 1.5X 10^8 TU/ml, and expression of SDK2-3xFlag was detected by Western Blot using Flag antibody (FIG. 19). From the data, the D08 truncated body can be judged to have certain bearing capacity on the large gene, the virus output is increased under the same condition, and the target gene expression can be detected.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, so as to understand the technical solutions of the present invention specifically and in detail, but not to be understood as the limitation of the patent protection scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the patent of the invention is subject to the appended claims, and the description can be used for explaining the contents of the claims.

Sequence listing

<110> and Yuan Biotechnology (Shanghai) Ltd

<120> lentiviral vectors, systems and uses thereof

<160> 14

<170> SIPOSequenceListing 1.0

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ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1200

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1260

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1320

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1380

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 1440

tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 1500

atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 1560

cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat 1620

agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat 1680

tcaaaatttt cgggtttatt acagggacag cagagatcca 1720

<210> 2

<211> 1646

<212> DNA

<213> Artificial Sequence

<400> 2

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1200

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1260

caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1320

tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1380

gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 1440

tcaccattat cgtttcagac ccacctccca accccgaggg gaccctcgac ggtatcgcct 1500

ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 1560

caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg 1620

tttattacag ggacagcaga gatcca 1646

<210> 3

<211> 1608

<212> DNA

<213> Artificial Sequence

<400> 3

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1200

agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1260

caagaaaaga atgaacaaga attattggaa ttaaaattgg ctgtggtata taaaattatt 1320

cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt 1380

gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag 1440

gggaccctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg 1500

gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 1560

acaaaaattc aaaattttcg ggtttattac agggacagca gagatcca 1608

<210> 4

<211> 1558

<212> DNA

<213> Artificial Sequence

<400> 4

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1200

agagaaatta acaattacac aagcttaata cactccttaa ttgaaattgg ctgtggtata 1260

taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac 1320

tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc 1380

caaccccgag gggaccctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta 1440

cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca 1500

aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatcca 1558

<210> 5

<211> 1508

<212> DNA

<213> Artificial Sequence

<400> 5

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggaaaattgg 1200

ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1260

tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1320

acccacctcc caaccccgag gggaccctcg acggtatcgc ctttaaaaga aaagggggga 1380

ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 1440

aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 1500

gagatcca 1508

<210> 6

<211> 1458

<212> DNA

<213> Artificial Sequence

<400> 6

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1140

agtaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1200

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1260

atcgtttcag acccacctcc caaccccgag gggaccctcg acggtatcgc ctttaaaaga 1320

aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac 1380

atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac 1440

agggacagca gagatcca 1458

<210> 7

<211> 1408

<212> DNA

<213> Artificial Sequence

<400> 7

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga 720

ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 780

ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 840

gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 900

gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 960

aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1020

atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1080

ctggggattt gggaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct 1140

tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat 1200

attcaccatt atcgtttcag acccacctcc caaccccgag gggaccctcg acggtatcgc 1260

ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat 1320

agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg 1380

ggtttattac agggacagca gagatcca 1408

<210> 8

<211> 1411

<212> DNA

<213> Artificial Sequence

<400> 8

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggccgt gaattatata aatataaagt 720

agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag 780

agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag 840

cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat 900

agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact 960

cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa 1020

ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt 1080

gccttggaat gctagtaaat tggctgtggt atataaaatt attcataatg atagtaggag 1140

gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg 1200

gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc tcgacggtat 1260

cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1320

aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1380

tcgggtttat tacagggaca gcagagatcc a 1411

<210> 9

<211> 1361

<212> DNA

<213> Artificial Sequence

<400> 9

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 660

aaacaaaagt aagaccaccg cacagcaagc ggccggcccc caccaaggca aagagaagag 720

tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 780

cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 840

tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 900

tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 960

gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 1020

ccactgctgt gccttggaat gctagtaaat tggctgtggt atataaaatt attcataatg 1080

atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga 1140

gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc 1200

tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 1260

tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa 1320

ttcaaaattt tcgggtttat tacagggaca gcagagatcc a 1361

<210> 10

<211> 1361

<212> DNA

<213> Artificial Sequence

<400> 10

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 600

gcatcaaagg atagagataa aagacaccaa ggaagcttta ggccggccgt gaattatata 660

aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 720

tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 780

cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 840

tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 900

tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 960

gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 1020

ccactgctgt gccttggaat gctagtaaat tggctgtggt atataaaatt attcataatg 1080

atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga 1140

gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc 1200

tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa 1260

tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa 1320

ttcaaaattt tcgggtttat tacagggaca gcagagatcc a 1361

<210> 11

<211> 1311

<212> DNA

<213> Artificial Sequence

<400> 11

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc ggccggccgt 600

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 660

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 720

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 780

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 840

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 900

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 960

ctcatttgca ccactgctgt gccttggaat gctagtaaat tggctgtggt atataaaatt 1020

attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat 1080

agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc 1140

gaggggaccc tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca 1200

ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 1260

attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc a 1311

<210> 12

<211> 1261

<212> DNA

<213> Artificial Sequence

<400> 12

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 540

ggccggccgt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc 600

caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt 660

gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac 720

ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc 780

tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc 840

aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg 900

ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagtaaat tggctgtggt 960

atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg 1020

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc 1080

tcccaacccc gaggggaccc tcgacggtat cgcctttaaa agaaaagggg ggattggggg 1140

gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 1200

acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 1260

a 1261

<210> 13

<211> 1211

<212> DNA

<213> Artificial Sequence

<400> 13

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 240

gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 300

aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 360

gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 420

atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 480

tggcctgtta ggccggccgt gaattatata aatataaagt agtaaaaatt gaaccattag 540

gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa 600

taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa 660

tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt 720

tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc 780

agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga 840

tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagtaaat 900

tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 960

gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1020

cagacccacc tcccaacccc gaggggaccc tcgacggtat cgcctttaaa agaaaagggg 1080

ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 1140

ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 1200

gcagagatcc a 1211

<210> 14

<211> 1211

<212> DNA

<213> Artificial Sequence

<400> 14

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 60

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 120

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 180

gtggcgcccg aacagggact gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 240

tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg 300

tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg 360

gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg 420

cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac 480

aaccatccct ggccggccgt gaattatata aatataaagt agtaaaaatt gaaccattag 540

gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa 600

taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa 660

tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt 720

tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc 780

agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga 840

tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagtaaat 900

tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 960

gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1020

cagacccacc tcccaacccc gaggggaccc tcgacggtat cgcctttaaa agaaaagggg 1080

ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 1140

ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 1200

gcagagatcc a 1211

Claims (10)

1. The lentivirus vector is characterized by having a truncated cis-acting element, and the sequence of the truncated cis-acting element is shown as SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6 or SEQ ID NO. 8.

2. The lentiviral vector of claim 1, wherein the truncated cis-acting element is linked at its 3' end to a gene segment of interest.

3. The lentiviral vector of claim 2, wherein the target gene fragment has a length ranging from 4500bp to 7700 bp.

4. The lentiviral vector of claim 3, wherein the gene fragment of interest is at least one selected from spCas9, dSpCas9, AsCpf1 and LbCpf 1.

5. A lentiviral vector system comprising a packaging component and the lentiviral vector of any one of claims 1 to 4.

6. The lentiviral vector system of claim 5, wherein the lentiviral vector system is a two-, three-, or four-plasmid system.

7. The lentiviral vector system of claim 5 or 6, wherein the resulting lentiviral particle is packaged.

8. Use of a lentiviral vector system according to claim 5 or 6 in the preparation of a medicament for gene therapy.

9. A host cell infected and transduced with the lentiviral vector system of claim 5 or 6.

10. A method of lentivirus transfection, characterized in that a system comprising the lentivirus vector of claim 5 or 6 is transferred into a host cell.

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