CN113832189A - gRNA for knocking out pig immunoglobulin heavy chain IGHG region and application thereof - Google Patents
gRNA for knocking out pig immunoglobulin heavy chain IGHG region and application thereof Download PDFInfo
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- CN113832189A CN113832189A CN202111037414.8A CN202111037414A CN113832189A CN 113832189 A CN113832189 A CN 113832189A CN 202111037414 A CN202111037414 A CN 202111037414A CN 113832189 A CN113832189 A CN 113832189A
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Abstract
The invention discloses a gRNA for knocking out a pig immunoglobulin heavy chain IGHG region and application thereof. One technical scheme to be protected by the invention is a method for knocking out a porcine immunoglobulin heavy chain IGHG region, which comprises introducing a substance for reducing or inhibiting the activity or content of a protein of the IGHG region of a porcine immunoglobulin heavy chain constant region or a substance for regulating the expression of a gene encoding the protein into a porcine cell. According to the invention, the characteristic of multiple repetitive sequences of the pig immunoglobulin heavy chain IGHG region is utilized, multiple cutting of multiple sites of the IGHG region is realized by using the CRISPR-Cas9 technology of 4 gRNAs combined with nucleic acid molecules shown in the sequence 2 in the sequence table, and the simultaneous knockout of the IGHG region with the length of 110kb sequence, which influences the antibody immunogenicity, of the immunoglobulin heavy chain constant region in the pig is finally realized, so that a foundation is laid for establishing a human immunoglobulin transgenic pig.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a gRNA for knocking out a pig immunoglobulin heavy chain IGHG area and application thereof.
Background
Therapeutic antibodies are of great significance in the treatment and prevention of various pathogen infections. However, the traditional antibody development requires antibody screening, affinity maturation, humanization, production and preparation, and other links, which are time-consuming and long-lasting, and particularly, it is difficult to achieve a rapid response effect for infection treatment of some new pathogens or mutant pathogens.
The antibody drug has great significance in the aspects of treating and preventing various pathogen infections, namely life, and the key of the research and development of the drug is to develop and produce the antibody drug in a sufficient amount in a shortest time. The traditional antibody research and development needs antibody screening, affinity maturation, humanization, production preparation and other links, the time consumption is long, and especially for infection treatment of some novel pathogens or mutant pathogens, the effect of rapid response is difficult to achieve. The use of horse serum for the treatment of infections caused by pathogens has long been used, with the main risk that antibodies produced by horse serum may be strongly immunogenic, limiting their widespread use to some extent. Introduction of human immunoglobulin genes into the genomes of other animals and expression of various human antibodies in these animals has been a hotspot for the recent study of humanized antibodies. The endogenous immunoglobulin production system of the transgenic animal remains intact after replacement with the human-derived production system, the system for recognizing antigen and mobilizing antibody production. Because the antibody is generated in vivo and undergoes normal assembly and maturation processes, the synthesized antibody is very similar to the antibody synthesized by the human body, thereby ensuring that the finished product has higher target affinity. By the end of 2017, the FDA (united states food and drug administration) approved 25 fully human antibody drugs on the market, 18 of which were derived from human antibody transgenic animals with annual output values exceeding billions of dollars, reflecting that fully human antibody drugs are the trend of future antibody development and the highest point of biopharmaceutical production, and humanized antibody animals become strategic animals for the production of fully human antibody drugs.
In order to obtain fully human antibodies, mouse IgG is replaced by human IgG, and a few humanized antibodies such as XenoMouse, VelocImmune, Harbour mouse, kymab and OmniRat, mice and rats have been successfully cultivated worldwide and used for the production of fully human antibody drugs. Although the production of fully humanized antibodies using transgenic mice has become the mainstream in the field of antibody medicine at present, the mice are too small to obtain antibodies directly from blood. Large animals such as pigs, cows and the like are not limited by volume, and the production of antibodies in a pasture manner can be directly realized. Robt and his colleagues have demonstrated that human Ig loci can also be correctly rearranged and expressed in cattle. The Kyowa Hakko Kirin company of Japan breeds the immunoglobulin fully human transgenic cattle, but the adopted technology has certain defects, is difficult to stably inherit, and needs further improvement. The research on humanized antibody transgenic animals using rabbits, camels, chickens and pigs is currently being carried out by companies such as Therapeutic Human polypeptides Inc (THP), Ablynx, origin Therapeutics, Revivcor, etc., but is still in progress.
Pigs are evolutionarily closer to humans than mice and cows, and will have functions closer to humans. The piglet model has advantages over the rodent model because the development of the fetal antibody repertoire is not influenced by the mother, and furthermore, the pig model has more advantages over the bovine model: (1) short pregnancy and oestrus times, (2) large litter size, and (3) established specific methods for raising specifically pathogen-free and sterile pigs. The transgenic pig has wide application in agriculture and biomedicine. Knocking foreign genes into the genome of pig somatic cells has been extremely difficult because of the extremely low efficiency of conventional Homologous Recombination (HR). The zinc finger nuclease technology, TALEN technology and CRISPR/Cas9 technology create a brand new genome editing era and overcome the defects of high difficulty, low editing efficiency, high cost and the like of the traditional gene targeting technology. The CRISPR/Cas9 technology is applied to gene editing research in 2013, is more convenient to construct and use, has higher efficiency, can realize multiple gene editing researches at one time due to higher gene targeting efficiency, and is widely applied due to lower cost.
Disclosure of Invention
The technical problem to be solved by the invention is how to knock out the genome sequence of the pig immunoglobulin heavy chain constant region IGHG region.
In order to solve the technical problems, the invention firstly provides a method for knocking out the heavy chain of the pig immunoglobulin. The method comprises introducing into a porcine cell a substance that reduces or inhibits the activity or content of a protein in the IGHG region of a porcine immunoglobulin heavy chain constant region or a substance that modulates the expression of a gene encoding said protein.
The substance which reduces or inhibits the activity or content of the protein of the IGHG region of the porcine immunoglobulin heavy chain constant region or the substance which regulates the expression of the gene encoding said protein as described above may be any of the following substances c1) -c 4):
c1) a substance that inhibits or reduces the activity or content of a protein in the IGHG region of the porcine immunoglobulin heavy chain constant region or a nucleic acid molecule that regulates the expression of a gene encoding said protein. The nucleic acid molecule may be a DNA molecule expressing a gRNA targeting a protein encoding gene of the IGHG region of the porcine immunoglobulin heavy chain constant region or a gRNA targeting a protein encoding gene of the IGHG region of the porcine immunoglobulin heavy chain constant region.
c2) An expression cassette comprising the nucleic acid molecule according to c 1).
c3) A recombinant vector comprising the nucleic acid molecule according to c1) or a recombinant vector comprising the expression cassette according to c 2).
c4) A recombinant microorganism containing c1) the nucleic acid molecule, or a recombinant microorganism containing c2) the expression cassette, or a recombinant microorganism containing c3) the recombinant vector.
The nucleic acid molecule described above under c1) may be a DNA molecule as shown under b1) or b 2):
b1) and the DNA molecule shown in the sequence 2 in the sequence table.
b2) And a DNA molecule having 90% or more than 90% identity with the nucleotide sequence defined in b 1).
In the above methods, the term "identity" refers to sequence similarity to a native nucleic acid sequence. Identity can be assessed visually or by computer software. Using computer software, the identity between two or more sequences can be expressed in percent (%), which can be used to assess the identity between related sequences. The 90% or greater identity can be at least 91%, 92%, 93%, 94%, 95%, 96%, 98%, or 99% identity.
The grnas described above may include 4 different grnas: gRNA1, gRNA2, gRNA3, and gRNA 4. The DNA sequence of the gRNA1 can be shown as 271-290 th nucleotide of the sequence 2 in the sequence table. The DNA sequence of the gRNA2 for coding can be shown as 629-648 nucleotides in the sequence 2 of the sequence table. The DNA sequence of the gRNA3 can be shown as 987-1006 th nucleotide of the sequence 2 in the sequence table. The encoding DNA sequence of the gRNA4 can be shown as the 1345-1364 th nucleotide of the sequence 2 in the sequence table.
The target site location targeted by the gRNA1 on the pig genome described above may be B1, the target site location targeted by the gRNA2 on the pig genome may be B2, and the target site locations targeted by the gRNA3 and the gRNA4 on the pig genome may be B3 and B4.
The B1 target site sequence may correspond to the nucleotide sequence at positions 2082-2101, 23121-23140, 44503-44522, 66711-66730, 91639-91658 and 110035-110054 of the sequence 1 in the sequence table. The target site sequence B2 may correspond to the nucleotides 2207-2226, 23246-23265, 44628-44647, 66836-66855, 91764-91783 and 110160-110179 of sequence 1 in the sequence table. The B3 target site sequence may correspond to nucleotides 3806-3825, 68447-68466 and 111735-111754 of sequence 1 in the sequence table. The B4 target site sequence may correspond to nucleotides 3805-3824, 68446-68465 and 111734-111753 of sequence 1 in the sequence table.
The above-mentioned inhibition or reduction of the activity or content of the protein in the IGHG region of the heavy chain constant region of porcine immunoglobulin or the regulation of the expression of the gene encoding the protein can be achieved by any means known in the art, such that the gene is subjected to deletion mutation, insertion mutation or base change mutation, thereby achieving the reduction or loss of the gene function, specifically, chemical mutagenesis, physical mutagenesis, RNAi, genome site-directed editing or homologous recombination, etc.
In the above-mentioned genome site-directed editing method, Zinc Finger Nuclease (ZFN) technology, Transcription activator-like effector nuclease (TALEN) technology, Clustered regularly spaced short palindromic repeats and their related systems (Clustered regularly interspaced short palindromic repeats/CRISPR associated, CRISPR/Cas9 system) technology, and other technologies capable of realizing genome site-directed editing can be used. In any case, the entire gene encoding the protein described above may be targeted, and each element regulating the expression of the gene encoding the protein described above may be targeted, as long as the loss or reduction of the function of the gene can be achieved. For example, the exon or 5' UTR of the gene encoding the protein described above may be targeted.
The nucleotide sequence of the IGHG region of the porcine immunoglobulin heavy chain constant region described above can be represented by sequence 1 in the sequence listing.
In the above method, the reducing or inhibiting the activity or content of the protein of the IGHG region of the porcine immunoglobulin heavy chain constant region or regulating the expression of the gene encoding the protein may be performed by subjecting the porcine genomic sequence shown in sequence 1 in the sequence table to the following mutations:
the nucleotide 1425 th to 111271 th in the sequence 1 in the sequence table in the pig genome is deleted.
The method can specifically be that the nucleotide sequence of the IGHG region of the porcine immunoglobulin heavy chain constant region is subjected to site-directed editing by the CRISPR/Cas9 method, so that the expression level of the protein coding gene of the IGHG region in the porcine cells is reduced or inhibited, for example, a recombinant plasmid vector expressing Cas9 protein and gRNA is infected by lentivirus to realize the transfer and integration of a foreign gene into the porcine cells. The porcine cells can be porcine kidney cells.
In order to solve the above technical problem, the present invention also provides the nucleic acid molecule as described above under c 1).
In order to solve the above technical problems, the present invention also provides an expression cassette comprising the above-described nucleic acid molecule.
In order to solve the above technical problem, the present invention also provides a recombinant vector comprising the above-described nucleic acid molecule and/or the above-described expression cassette.
In order to solve the above technical problem, the present invention also provides a recombinant microorganism containing the above-described nucleic acid molecule and/or the above-described expression cassette and/or the above-described recombinant vector.
Recombinant cell lines containing the above-described nucleic acid molecules and/or the above-described expression cassettes and/or the above-described recombinant vectors and/or the above-described recombinant microorganisms are also within the scope of the present invention.
In order to solve the above technical problem, the present invention also provides any one of the following uses of the nucleic acid molecule as described above and/or the expression cassette as described above and/or the recombinant vector as described above and/or the recombinant cell line of the recombinant microorganism as described above:
A1) use in the knock-out of a porcine immunoglobulin heavy chain and/or knock-out of a porcine immunoglobulin locus;
A2) application in preparation of pig model with immunoglobulin heavy chain knockout and/or immunoglobulin locus knockout
A3) The application in establishing human immunoglobulin transgenic pigs;
A4) the application in preparing human antibody.
Also within the scope of the present invention are products of recombinant cell lines comprising the above-described nucleic acid molecules and/or the above-described expression cassettes and/or the above-described recombinant vectors and/or the above-described recombinant microorganisms.
The invention uses CRISPR-Cas9 technology to knock out IGHG area which affects the antibody immunogenicity of immunoglobulin heavy chain constant region in pig, uses the characteristic of multiple repetitive sequences in the area to design a mode of using 4 gRNAs (gRNA1-gRNA4) to realize multiple cutting of multiple sites, and the sequencing result of the amplified fragment TA clone of the obtained 13 # clone of knock-out cell shows that the genome sequence of pig IGHG area is cut from 1425 site to 111271 site, which indicates that the gRNA in the clone plays a role in successfully knocking out gene fragment, deletes the 1425-111271 bit nucleotide of sequence 1 in the sequence table, namely IGHG3, IGHG5-1, IGHG5-2, IGHG1, IGHG6-1 and IGHG6-2 genes of IGHG area in pig immunoglobulin heavy chain area, thereby successfully knocking out IGHG area with 110kb length in pig genome sequence, realizing hundred kb knock-out of mammalian cell with 4 gRNAs acting together, lays a foundation for establishing the human immunoglobulin transgenic pig.
Drawings
Fig. 1 is a schematic representation of gRNA positions designed for the porcine immunoglobulin heavy chain constant region IGHG portion. Wherein B1, B2, and B3 represent the location of the designed gRNA targeting gene; since B3 differs from B4 by only one base and the knockout position is the same, the position of the B4 target site is not labeled in the legend.
FIG. 2 shows the result of electrophoretic verification of standard gRNA shearing efficiency by spCas9-gRNA target efficiency detection kit (VK007, Beijing Weishanglide Biotech Co., Ltd.). M is 5000bp DNA ladder (Beijing prilley gene technology, Inc.) 1,2 is a DNA fragment containing a gRNA target; 3: standard gRNA1 shearing effect: the shear efficiency was 70% 4: standard gRNA2 shearing effect: the shear efficiency was 20%.
FIG. 3 shows the results of electrophoretic verification of the cleavage efficiency of 4 gRNAs. M: marker III (Tiangen Biochemical technology (Beijing) Ltd.); 1: obtaining a dsDNA1 fragment containing B1 and B2 sites by amplification of a primer pair, wherein the size is 925 bp;
2: cutting by using a gRNA1dsDNA1 fragment, wherein the sizes of the cut fragments are 497bp and 428 bp;
3: cutting a dsDNA1 fragment by using gRNA2, wherein the sizes of the cut fragments are 622bp and 303 bp;
4: obtaining a dsDNA2 fragment containing B1 and B2 sites by amplification of a primer pair, wherein the size is 828 bp;
5: cutting a dsDNA2 fragment by using gRNA1, wherein the sizes of the cut fragments are 490bp and 338 bp;
6: cutting a dsDNA2 fragment by using gRNA2, wherein the sizes of the cut fragments are 615bp and 213 bp;
7: obtaining a dsDNA3 fragment containing B3 and B4 sites by amplification of a primer pair, wherein the size is 716 bp;
8: cutting a dsDNA3 fragment by using gRNA3, wherein the sizes of the cut fragments are 152bp and 564 bp;
9: the dsDNA3 fragment was cleaved with gRNA4 to a size of 152bp and 564 bp.
FIG. 4 shows the construction of GV504-4 gRNA.
FIG. 5 is the electrophoretogram of the digested vector and original plasmid. 1 is an electrophoresis band corresponding to the original plasmid; 2 the original plasmid vector is an electrophoretic band after enzyme digestion.
FIG. 6 is an electropherogram of PCR identifying positive clones. 1-8# are different transformants, respectively.
Figure 7 is a PCR identification of expression of Cas9, a cell positive clone. The 1# template is PK15 cell genome, and the 2-8# is different positive clone genome.
FIG. 8 shows Western Blot to detect the expression of Cas9 protein as a positive cell clone. PK15 cells # 1-6 were different positive monoclonals of PK15 cells stably transfected Cas 9.
FIG. 9 shows the fluorescence detection of positive clones of PK15-Cas9 transfected with GV504-4 gRNA. The top left is the bright field view of clone 1, the top right is the fluorescent field view of clone 1, the bottom left is the bright field view of clone 2, and the bottom right is the fluorescent field view.
FIG. 10 shows an electrophoretogram after amplification using LJ3FR, LJ4FR, and LJ5 FR. Size of PCR product without splicing: the fragment obtained by amplification with primer pair LJ3FR has a size of 2970 bp. The size of the fragment obtained by amplification of the primer pair LJ4FR is 2982 bp; the amplified fragment size of the primer pair LJ5FR is 2978 bp. The 1# template is PK15-CAS9 cell genome, and the 2-24# template is 23 cell monoclonal genomes of PK15-Cas9 transfected with GV504-4 gRNA.
FIG. 11 shows the alignment of the sequence of the clone No. 13 sequencing splice with the sequence of the porcine IGHG region.
Fig. 12 is a schematic of the 4gRNA knockout IGHG loci. Wherein B1, B2, and B3 represent different gRNA targets designed to target different positions of the IGHG locus; since B3 differs from B4 by only one base and the knockout position is the same, the position of B4 is not labeled in the legend.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.
The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The GV504 vector is from Shanghai Jikai gene science and technology limited;
PK15 cells (porcine kidney cells) were derived from shanghai gening biotechnology limited;
the lentivirus is from Shanghai Jikai gene science and technology limited;
Lenti-Cas9-puro is a product of Shanghai Jikai gene technology Co., Ltd, the product number is 7768-1;
BCA protein quantification kit (Thermo fisher 23225);
a murine anti-cas 9 monoclonal antibody (Cell signaling technology, # 14697);
rabbit anti-GAPDH monoclonal antibody (bosch biosome, a 00227-1);
HRP-labeled goat anti-mouse IgG (sequoia jessamine ZB 2305);
HRP-labeled goat anti-rabbit IgG (sequoia jessamine ZB 2301);
SuperECL Plus hypersensitive luminescent liquid (PE company 275-;
Bio-Rad ChemDoc chemiluminescent imaging System (Bole Life medicine products, Inc.);
example I construction of CRISPR/Cas9 vector and obtaining of porcine immunoglobulin locus knockout cell line
Because of the huge immunoglobulin locus gene, in order to successfully replace the pig immunoglobulin gene sequence with the human immunoglobulin locus, the pig immunoglobulin locus should be knocked out first. The pig immunoglobulin heavy chain locus is located on chromosome 7, the heavy chain variable region is composed of nearly 30 VH, 5 DH and JH gene segments, and each VH gene has extensive repetitive sequences. The heavy chain constant region is about 190kb in length, consistent with that of other mammals. The porcine IGHC gene cluster comprises 10 genes, IGHM, IGHD, 6 IGHG genes, IGHE and IGHA. The kappa chain is located on chromosome 3q 12-q 14, and the GK gene is distributed on a genome scaffold, wherein 26V region genes and 1C region gene have the total length of about 290 kb. The lamda strand is located on chromosome 14, chromosome 14q 16-q 21, approximately 250kb in length, with two distinct clusters, containing 22 IGLV genes, less than human (52) and bovine (25). In order to eliminate the immunogenicity of the transgenic pig, the invention uses CRISPR/Cas9 technology to knock out IGHG region of immunoglobulin heavy chain constant region of pig, wherein the region is as long as 110 kb.
Design of sgRNA and detection of in vitro cleavage Activity
1.1 design of sgRNA
The sequence analysis of the IGHG region of the immunoglobulin heavy chain constant region of the pig shows that the region has a large number of repetitive sequences, so the invention designs the following four sgRNA targets to realize the simultaneous cutting of multiple targets and multiple sites:
the nucleotide sequences of the two upstream targets are as follows:
gRNA1:5’-AGCTGCAATGGCAGCGAGCT-3’；
gRNA2:5’-AGGAGGCTGCGTGACCGCCA-3’；
the nucleotide sequences of the two downstream targets are as follows:
gRNA3:5’-AGCGCTGTGTACATACGTCC-3’；
gRNA4:5’-GACGTATGTACACAGCGCTG-3’
the gRNA coding DNA aiming at the four target points is constructed into the GV504 vector, and because the heavy chain constant region of the pig immunoglobulin has a large number of repetitive sequences, the four target points can effectively cut aiming at the (IGHG region) sequence so as to achieve the aim of completely knocking out the sequence. A schematic position diagram of four target targeting IGHG regions is shown in fig. 1, B1 represents that gRNA1 targets a target site position of a genome, B2 represents that gRNA2 targets a target site position of a genome, B3 and B4 represent that gRNA3 and gRNA4 target site positions of a genome, respectively, and B4 and B3 target site positions are the same (B4 is located on a complementary strand of B3, and is different from the complementary strand of B3 by only two nucleotides), and B4 is not shown.
The genomic sequence of the pig genome obtained by sequencing three clones (ACCESSION: AB699686 Sus scrofa genes for immunoglubulin heavyway variable and constant regions, clone: L264P 10; ACCESSION: AB699687 Sus scrofa genes for immunoglubulin heavyway constant regions, clone: L342C 21; ACCESSION: AB699688 Sus scrofa genes for immunoglubulin heavyway constant region, clone: L380J14) on NCBI was spliced to obtain the genomic sequence of the pig heavy chain constant region IGHG region (sequence 1 in the sequence listing).
The B1 target site sequence corresponds to the nucleotide at positions 2082-2101, 23121-23140, 44503-44522, 66711-66730, 91639-91658 and 110035-110054 of the sequence 1 in the sequence table; the target site sequence B2 corresponds to the nucleotides 2207-2226, 23246-23265, 44628-44647, 66836-66855, 91764-91783 and 110160-110179 of the sequence 1 in the sequence table; the B3 target site sequence corresponds to nucleotides 3806-3825, 68447-68466 and 111735-111754 of the sequence 1 in the sequence table; the B4 target site sequence corresponds to nucleotide 3805-3824, 68446-68465 and 111734-111753 of sequence 1 in the sequence table.
1.2 in vitro amplification to obtain DNA fragment containing gRNA target sequence
1.2.1 and taking genome DNA of PK15 cells as a template, respectively amplifying DNA fragments containing 4gRNA target sequences, wherein the DNA fragments are used for detecting the shearing efficiency of the gRNAs. Primers Primer-F and Primer-R for amplifying DNA fragments containing target sequences are designed as follows:
BA F1：5’-CTCTGGGGAAAGCTGCCTC-3’
BA R1：5’-CAACACGCTTGTCCACCTTG-3’
BA F2：5’-GAAAGCTGCCTCGGGTGAG-3’
BA R2：5’-CCATGCTGCTGAGGGAGTAG-3’
BA F3：5’-TATTCCAGTGTGCGGTGATG-3’
BA R3:5’GTGAGTGTTGCCACGAGTG-3’
the size of dsDNA1 of the BA F1 and BA R1 amplified fragments is 925bp, and the dsDNA contains nucleotide sequences of B1 and B2 targets; the size of dsDNA2 of the BA F2 and BA R2 amplified fragments is 828bp, and the dsDNA contains nucleotide sequences of B1 and B2 targets; the amplified fragment dsDNA3 of BA F3 and BA R3 is 716bp and contains the nucleotide sequences of B3 and B4 targets.
1.2.2 PCR amplification System
1.2.3 PCR amplification conditions
The PCR reaction conditions were as follows:
1.2.4 the PCR product is cut and recovered, and the obtained DNA fragment containing gRNA target sequence is purified.
1.3 in vitro transcription to obtain gRNAs
1.3.1 acquisition of gRNAPCR product
A. Primers T7-gRNA-FPg and gRNA-RP were synthesized for in vitro transcription:
gRNA-RP：5’-AGCACCGACTCGGTGCCACTT-3’
note: primers were designed with a gRNA target (underlined) (without PAM sequence) in the middle.
Wherein, the amplification products of the T7-gRNA1-FP and gRNA-RP primers are coding DNA of gRNA 1; the amplification products of the T7-gRNA2-FP and gRNA-RP primers are coding DNA containing gRNA 2; the amplification products of the T7-gRNA3-FP and gRNA-RP primers are coding DNA containing gRNA 3; the amplification products of the T7-gRNA4-FP and gRNA-RP primers are DNA encoding gRNA 4.
B, PCR amplification reaction: PCR (PCR product of about 120 bp) was performed using a T7-gRNA-FPg (diluted at 10. mu.M) and a gRNA-RP primer pair and a standard gRNA template (Beijing Vital bioscience Co., Ltd.) as a template, and 2 tubes were amplified in the following 50. mu.L system for each sample.
The PCR system is as follows:
1.3.2 transcription step of gRNA (note that the tip of RNasefree and EP tube were used):
the in vitro transcription system is as follows: all components come from a kit spCas9-gRNA target efficiency detection kit (VK007, Beijing Weishanglide Biotech limited)
The reaction system is as follows:
after mixing, the reaction was carried out at 37 ℃ overnight (treatment with a 37 ℃ incubator, with as little water bath as possible, is recommended).
1.4 recovery and extraction of gRNA
1) After the reaction, 2. mu.l of DNaseI (VK010-05) was added and the reaction was carried out at 37 ℃ for 40-60 min.
2) Adding 112 mu of LDEPC water (VK010-06) and 18 mu of LStop Solution (VK010-04), mixing, adding 2 times of volume (300 mu L) of absolute ethyl alcohol (self-prepared, no RNase pollution is noticed), fully mixing, and freezing at-20 ℃ for more than 3 hours (generally overnight treatment).
3) The mixture was centrifuged at 13000rpm at 4 ℃ for 20min, and the supernatant was removed to leave a precipitate.
4) Adding 450 mu L of 70% cold ethanol for cleaning (self-prepared, attention is paid to preparation by DEPC water), centrifuging at 13000rpm and 4 ℃ for 15min, removing supernatant, retaining precipitate, centrifuging at 13000rpm and 4 ℃ for 1min, carefully sucking residual liquid, airing at room temperature for 1-2 min, adding 40 mu L of DEPC water to dissolve RNA precipitate, and respectively obtaining four gRNAs: gRNA1, gRNA2, gRNA3, and gRNA 4.
5) And (3) measuring the concentration of the gRNA (generally 300-800 ng/mu L, if the concentration is too high, taking 1 mu L to perform agarose gel electrophoresis detection to judge whether the brightness of the strip is consistent with the measured concentration), and directly using the strip for a subsequent spCas9/gRNA enzyme digestion experiment after determining that the concentration is correct, or storing the strip at-80 ℃ for later use.
1.5 spCas9/gRNA in vitro enzyme digestion reaction
Measuring the shearing activity of 4 gRNAs through in vitro enzyme digestion reaction, loading samples according to the following reaction system sequence, and preparing enzyme digestion reaction:
note: the "sample gRNA recovered in step 1.4" is gRNA1, gRNA2, gRNA3 or gRNA 4. "dsDNA for enzyme digestion" is dsDNA1, dsDNA2 or dsDNA3 fragment obtained in step 1.2.1, the dsDNA cut by gRNA1 and gRNA2 is dsDNA1 and dsDNA2, and the dsDNA cut by gRNA3 and gRNA4 is dsDNA 3. "X" represents the amount of water required to replenish the 20. mu.L total system.
After fully mixing, reacting at 37 ℃ for 30min, adding 3 mu L of DNALLOADING Buffer, mixing, boiling at 65 ℃ for 5min, and detecting and analyzing the enzyme digestion result by running 2% agarose gel electrophoresis.
The results shown in fig. 2 are standard gRNA cleavage efficiencies provided by spCas9-gRNA target efficiency detection kits, as shown by the cleavage efficiency of standard gRNA1 being 20% (lane 3 in fig. 2) and the cleavage efficiency of standard gRNA2 being 70% (lane 4 in fig. 2).
The in vitro enzyme digestion reaction result is shown in fig. 3, the in vitro shearing activity of four gRNAs designed in the pig immunoglobulin constant region IGHG region is over 50%, which shows that the gRNA target points of four samples have good activity and can be used.
2. Construction of GV504-4gRNA expression vector
2.1 gRNA sequence design
For simultaneous expression of 4 gRNAs, each gRNA was preceded by U6 promoter and followed by RNA scaffold sequence, 4 gRNAs with respective expression frameworks were ligated in tandem to the same vector GV504 (FIG. 4), and the coding sequence U6-gRNA1-U6-gRNA2-U6-gRNA3-U6-gRNA4 with 4 gRNAs in tandem was synthesized as shown in sequence 2 in the sequence Listing. The 1 st to 6 th nucleotides of the sequence 2 in the sequence table are NheI restriction endonuclease recognition site sequences, and the 1468 th and 1475 th nucleotides are PacI restriction endonuclease recognition sites; the nucleotides 21-261, 379-619, 734-977 and 1095-1336 of the sequence 2 in the sequence table are all U6 promoter sequences; the 271-th and 290-th nucleotides of the sequence 2 in the sequence table are the coding DNA sequence of the gRNA1, the 629-th and 648-th nucleotides of the sequence 2 in the sequence table are the coding DNA sequence of the gRNA2, the 987-th and 1006-th nucleotides of the sequence 2 in the sequence table are the coding DNA sequence of the gRNA3, and the 1345-1364-th nucleotides of the sequence 2 in the sequence table are the coding DNA sequence of the gRNA 4; the 291-position 366, 649-position 724, 1007-position 1082, 1365-position 1440 nucleotides of the sequence 2 in the sequence table are RNA scaffold sequences.
2.2 construction of GV504-4gRNA expression vector
2.2.1 construction of GV504-4gRNA expression vector
Digesting the plasmid (sequence 2 in the sequence table) containing 4gRNA coding DNAs chemically synthesized in the step 2.1 by NheI and PacI to obtain a plasmid digestion product; meanwhile, NheI and PacI are used for enzyme digestion of the GV504 vector to obtain a linearized GV504 vector (figure 5), and the obtained plasmid enzyme digestion product and the linearized GV504 vector are connected overnight by using T4 ligase to obtain a connection product, namely the recombinant plasmid GV504-4 gRNA. The recombinant plasmid GV504-4gRNA is a recombinant vector obtained by replacing a small segment between NheI and PacI enzyme cutting recognition sites on the GV504 vector with nucleotides shown in a sequence 2 in a sequence table and keeping other nucleotide sequences of the GV504 vector unchanged.
The GV504-4gRNA contains U6-gRNA1-U6-gRNA2-U6-gRNA3-U6-gRNA4 shown in a sequence 2 in a sequence table, and can simultaneously express 4 gRNAs.
The restriction enzyme reaction system of the vector is restricted by PacI/NheI:
| purified DNA plasmid (1. mu.g/. mu.l) | 2μL | |
| NheI(20U/μl) | 1μL | |
| PacI(20U/μl) | 1μL | |
| 10× | 5μL | |
| 100×BSA | 0.5μL | |
| ddH2O | 40.5μL | |
| Total | 50μL |
The mixed reaction was left at 37 ℃ for 2 h.
2.2.2 design of PCR identification primers
Identifying primer P1: 5'-GACGTATGTACACAGCGCTG-3'
Identifying primer P2: 5'-GGAAAGAATAGTAGACATAATAGC-3'
2.2.3 PCR identification of recombinant clones
PCR amplification using P1 and P2 primer pairs identified recombinant plasmid GV504-4gRNA, positive transformant PCR product size (fig. 6): 246bp (shown as a sequence 4 in a sequence table).
2.2.4 sequencing results of positive clones:
the identified positive clone is sent to a sequencing company for sequencing verification, and the sequencing result shows that the recombinant plasmid GV504-4gRNA contains a coding sequence U6-gRNA1-U6-gRNA2-U6-gRNA3-U6-gRNA4 in which 4 gRNAs shown in a sequence 2 in a sequence table are connected in series, and the sequencing result is completely correct.
3. Construction of recombinant plasmid GV504-4gRNA Stable transfection cell Strain
3.1 Leti-Cas9-puro and GV504-4gRNA, lentiviral packaging CAS9 plasmid vectors
The process of lentivirus packaging CAS9 expression vector Leti-Cas9-puro and lentivirus packaging GV504-4gRNA is provided and completed by Shanghai Jikai Gene science and technology Co.
3.2 determination of the optimal concentration for puromycin screening
Lenti-Cas9-puro (expressing CAS9 protein) vector lentivirus packaging plasmid carries puromycin resistance gene, so the optimal concentration for puromycin screening needs to be determined first. When PK15 cells were grown to 80% confluence, complete media containing puromycin at concentrations of 0, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5. mu.g/mL was added. The death of PK15 cells was observed under a microscope, and the cells were all dead in complete medium at a concentration of 2. mu.g/mL after 72hr pressurization. Therefore, when selecting cells, the optimal concentration for puromycin selection was determined to be 2. mu.g/mL.
3.3 introduction of the Lenti-Cas9-Puro plasmid into PK15 cells Using lentiviruses
1) Inoculating cells: preparation of 3X 10-Density with complete Medium (MEM + 10% FBS + 1% double antibody: Medium obtained by adding FBS, penicillin and streptomycin to MEM, 10% FBS + 1% double antibody with FBS content of 10%, penicillin content of 100U/mL and streptomycin content of 10. mu.g/mL) was prepared4The suspension of PK15 cells in per mL is inoculated into a six-well plate and cultured for 16-24h at 37 ℃ until the cell confluency is 20-30%.
2) Preparing a virus transfection solution: 1mL of complete medium (MEM + 10% FBS: medium obtained by adding FBS to MEM, in MEM + 10% FBS, the content of FBS was 10%) was mixed with 40. mu.L of a transfection enhancing solution HiTrans G P solution (Kjekay Gene science Co., Ltd.) and 10. mu.L of lentivirus-packaged Lenti-Cas 9-Puro.
3) The culture medium in the six-well plate of 1) was aspirated, the virus transfection solution obtained in 2) above was added to PK15 cells, and the solution was changed in the middle of the procedure to maintain the viability of the cells.
4) When infection is carried out for 72hr, complete medium with puromycin concentration of 2 μ g/mL is added to carry out pressure screening on recombinant PK15-CAS9 cells introduced with Lenti-Cas9-Puro plasmid for 1-2 months.
3.4 identification of Positive clones in recombinant PK15-CAS9 cells
1) And (3) PCR identification: after the recombinant PK15-CAS9 cells obtained from the screening in 3.3 were filled in a cell bottle of T25, the cells were collected by centrifugation after trypsinization, washed once with PBS, and then resuspended in 200. mu.L of PBS, and 20. mu.L of proteinase K was added. Cell genomes were extracted using DNeasy Blood & Tissue kit (QIAGEN, 69504), and porcine endogenous reference gene actin was used as a control, and PCR was performed using the following primers to identify porcine endogenous reference gene actin and cas9 genes, respectively:
pig_actin_F：5’-TAACCGATCCTTTCAAGCATTT-3’
pig_actin_R：5’-TGGTTTCAAAGCTTGCATCATA-3’
Cas9-F：5’-TGGTGGAAGAGGATAAGAAGCA-3’
Cas9-R：5’-CAGGTCGTCGTCGTAGGTGTC-3’
wherein the pig _ actin _ F primer pair and the pig _ actin _ R primer pair are used for amplifying the pig reference gene actin; cas9-F and Cas9-R primer pairs were used to amplify Cas9 gene.
As shown in FIG. 7, the control PK15 cell (shown in lane 1 in FIG. 7) without the recombinant plasmid Lenti-Cas9-Puro and seven monoclonals (shown in lanes 2-8 in FIG. 7) of PK15-CAS9 cell with the recombinant plasmid Lenti-Cas9-Puro can detect the pig reference actin gene, and the size of the band is about 400 bp; however, the control PK15 cell does not detect the Cas9 gene, seven monoclonals of the PK15-CAS9 cell detect the Cas9 gene, and the size of the band is about 500 bp. The results demonstrate that Cas9 has been stably transfected into PK15 cells.
2) WB identified expression of CAS9 protein:
in order to prove the expression of the CAS9 protein, WB is used for detection, transfected PK15-CAS9 cells are cultured until the density reaches more than 90%, a culture solution is discarded, and PBS is used for washing for 3 times; adding Pierce IP lysate into a 6-well cell plate, and cracking the cells on ice for 30 minutes; collecting the cell lysate and centrifuging at 12000g for 15min at 4 ℃ to remove large cell debris; the BCA protein quantitative kit is used for determining the protein concentration in the cells; taking 1 mu g of protein released after cell lysis, adding 5 Xloading buffer solution, performing protein electrophoresis, performing membrane transfer, performing primary antibody labeling by using a mouse anti-CAS 9 monoclonal antibody and a rabbit anti-GAPDH monoclonal antibody, performing secondary antibody labeling by using HRP-labeled goat anti-mouse IgG and HRP-labeled goat anti-rabbit IgG, developing for 5 minutes by using SuperECL Plus hypersensitive luminescent solution, and taking a picture by using a Bio-Rad ChemDoc chemiluminescence imaging system.
As shown in FIG. 8, the control PK15 cells (shown in lane 1 in FIG. 8) without transfection of Cas9 recombinant plasmid Lenti-Cas9-Puro did not detect the expression of CAS9 protein, but only GAPDH, while PK15-CAS9 cells (shown in lanes 2-6 in FIG. 8) with transfection of recombinant plasmid Lenti-Cas9-Puro both detected the expression of CAS9 protein and rabbit anti-GAPDH-labeled GAPDH protein, indicating that the positive PK15-Cas9 cell clone stably expressed the CAS9 protein.
3.5 transfection of GV504-4gRNA into PK15-CAS9 cells Using lentiviruses
1) Inoculating cells: the preparation density was 3X 10 using the complete medium (MEM + 10% FBS + 1% double antibody: medium obtained by adding FBS, penicillin and streptomycin to MEM, 10% FBS + 1% double antibody, FBS content 10%, penicillin content 100U/mL, streptomycin content 10. mu.g/mL) to prepare a suspension having a density of 3X 104The suspension of PK15-CAS9 cells in each mL is inoculated into a six-well plate and cultured for 16-24h at 37 ℃ until the cell confluency is 20-30%.
2) Preparing a virus transfection solution: 1mL of complete medium (MEM + 10% FBS: medium obtained by adding FBS to MEM, in which MEM + 10% FBS was contained in an amount of 10%) was mixed with 40. mu.L of HiTrans G P solution (REVG005 Shanghai Jikai Gene science and technology Co., Ltd.) and 10. mu.L of lentivirus-packaged GV504-4gRNA to obtain a virus transfection solution.
3) Sucking the culture medium in the six-well plate in the step 1), adding the virus transfection solution in the step 2) into PK15-CAS9 cells, and changing the solution in the midway to keep the vitality of the cells.
4) Using a fluorescence inverted microscope to observe that only one clone in one hole of a 96-hole plate is marked with green fluorescence; after full growth, the cells are transferred to a 48-well plate, then to a 6-well plate, and then transferred to a cell bottle of T25, and a plurality of PK15-CAS9 cell clones PK15-CAS9-4gRNA (shown in figure 9) which stably transfer GV504-4gRNA are obtained.
Detection of cleavage of gRNA
4.1 design of identifying primers
Because the genome repetitive sequences of the pig IGHG regions are more, the invention compares the sequences by using multiple comparison software MEGX to find the following specific identification primers:
LJ3F：5'-GGTGAGCAGGGTCTAGGAGT-3'
LJ3R：5'-AAGAACCATGACAGCGTTGC-3'
LJ4F：5'-AGGTTGAACCTGGAGCTCCTG-3'
LJ4R：5'-GTGGTGTTGGGTGCTTTATTTCA-3'
LJ5F：5'-TGAGCAGGGTCTAGGAGTTCA-3'
LJ5R：5'-CTGCCTCGGAAAGAACCATGA-3'
wherein the primer pair LJ3F and LJ3R is used for detecting the shearing of the target sites of the gRNA pair B1\ B2\ B3\ B4, the amplified fragments have the sizes of 919-;
the primer pair LJ4F and LJ4R are used for detecting the shearing of the target sites of B1\ B2\ B3\ B4 by the gRNA, the amplified fragments have the sizes of 349-3858 nucleotides (3511bp), 349-24870 nucleotides (245252bp), 349-46252(45905bp), 349-68497 nucleotides (68149bp), 349-93415 nucleotides (93067bp), 349-785 nucleotides (111437bp), 877-3858 nucleotides (2982bp), 877-24870 nucleotides (23996bp), 111877-46252 nucleotides (45376bp), 877-68497 nucleotides (67621bp), 877-93415 nucleotides (92539bp), and 877-111785 nucleotides (110909bp) of the sequence 1 in the sequence table;
the primer pair LJ5F and LJ5R are used for detecting the shearing of the gRNA to the target sites of B1\ B2\ B3\ B4, and the amplification fragments have the sizes of six amplification products, namely, the 921-and 3898-th nucleotide (2978bp), the 921-and 24911-th nucleotide (23991bp), the 921-and 46292-th nucleotide (45372bp), the 921-and 68538-th nucleotide (67618bp), the 921-and 93455-th nucleotide (92535bp) and the 921-and 111826-th nucleotide (110906bp) in the sequence 1 in the sequence table;
the detection method comprises the following steps: if 4 gRNAs are not sheared, a large fragment in the amplification product of the primer pair cannot amplify the fragment by using DNA polymerase used in a PCR system, so that the result of PCR amplification product can only amplify to obtain the four small fragments (LJ3F and LJ3R primer pair: 2970bp, LJ4F and LJ4R primer pair: 3511bp, LJ4F and LJ4R primer pair: 2982bp, LJ5F and LJ5R primer pair: 2978bp), and the amplification product does not contain fragments with other sizes; if 4 gRNAs produce cleavage, the primer pair will produce other fragments in addition to the four small fragments in the amplification product.
4.2 PCR reaction System
4.3 PCR reaction conditions
4.4 TA cloning and sequencing identification
Performing nucleic acid electrophoresis on the fragments obtained by respectively using the three pairs of primers in the step 4.1 and PK15-CAS9-4gRNA monoclonal cell genomes as templates and the fragments obtained by using the control PK15-CAS9 cell genomes without the transformed recombinant plasmid GV504-4gRNA as templates; and constructing fragments of the two amplification products on a T carrier for sequencing, carrying out BLAST comparison on a sequence obtained by sequencing and a complete sequence of the IGHG region of the pig, and analyzing the shearing effect.
The amplification results of three pairs of primers LJ3F & LJ3R, LJ4F & LJ4R and LJ5F & LJ5R are shown in FIG. 10 (represented by LJ3, LJ4 and LJ5 in FIG. 10), wherein only four small fragments (2970 bp; 3511 bp; 2982 bp; 2978bp) are obtained but fragments of other sizes are not obtained (amplified bands using the control PK15-CAS9 cell as a template shown in lane 1 in FIG. 10), and the amplification results show that many amplified bands (2-24 shown in FIG. 10) using PK15-CAS9-4gRNA cell (23 clones) as a template amplify different fragments from the amplified bands (four small fragments) using the control PK15-CAS9 cell as a template, which indicates that all 23 clones of PK15-CAS9-4gRNA cells are cleaved to different degrees.
To confirm that the different degrees of cleavage of the 23 clones were due to the action of 4 gRNAs, the cleavage of each clone was recovered by gel cutting and sequenced after TA cloning. Cleavage sites were analyzed by performing a blast alignment of the sequencing results with the porcine IGHG region sequences using Bioedit software (https:// xiazai.zol.zol.com.cn/detail/48/470901. shtml).
As a result, it was found that the sequencing result (the splicing sequence of the amplified fragment) of the fragment obtained by PCR amplification of clone No. 13 of PK15-CAS9-4gRNA cell (lane 14 in FIG. 10) was compared with the genomic sequence of porcine IGHG region, which revealed: the 1199 th site and the 686 th site of the sequencing result correspond to the 912 nd site and the 1425 th site respectively shown in the pig IGHG region genome, namely sequence 1 in the sequence table, the 579 th site and the 101 th site of the sequencing result correspond to the 111749 th site and the 111271 th site respectively shown in the pig IGHG region genome, namely sequence 1 in the sequence table (in FIG. 11, Subject represents the pig IGHG region genome sequence, Query represents the spliced sequence of the sheared amplification fragment of the PK15-CAS9-4gRNA cell No. 13 clone), while the IGHG region genome sequence of the PK15-CAS9-4gRNA cell No. 13 clone is sheared from the 1425 th site to the 111271 th site in the middle region, which indicates that the clone is successfully subjected to gRNA knockout, namely the IGHG region genome sequence of the clone No. 13 is deleted of the 1115 th site 11178 th site nucleotide in the sequence 1 in comparison with the wild-type pig IGHG region genome sequence, namely IGHG3, IGHG 1-78-1 st site in the pig immunoglobulin heavy region, IGHG5-2, IGHG1, IGHG6-1 and IGHG6-2 genes, so that an IGHG region of 110kb is successfully knocked out, and the knock-out of a mammalian cell at the hundred kb level is realized by the combined action of 4 gRNAs (figure 12).
In conclusion, the IGHG region influencing the immunogenicity of the immunoglobulin heavy chain constant region antibody in the pig is knocked out by the CRISPR-Cas9 technology, the characteristic that the repetitive sequence of the region is multiple is utilized, the mode of combining 4 gRNAs is designed to realize multiple cutting at multiple sites, the simultaneous knocking-out of the pig genome sequence with the length of 110kb is realized, and the foundation is laid for establishing the humanized immunoglobulin transgenic pig.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
Sequence listing
<110> military medical research institute of military science institute of people's liberation force of China
<120> gRNA for knocking out pig immunoglobulin heavy chain IGHG region and application thereof
<130> GNCSQ211202
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 130062
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
gggggtggga gggcagggag cagtcctggc agctcctggg accaggtaga gtttgtgcag 60
gtgagcaggg gtgggtagga cagggggcag tcctggcagc tcctgggacc aggtagagtt 120
tgtgcaggtg agcaggggct gggaggacat ggggtagtcc tggtaccttc tgtgagcagg 180
tagagctagg gcaggtgagc agaagctgag aggaaacagt tgaacctgga gcttcttgaa 240
ccaggacgag cttgtacagg tgagcagggg ctgcaagggc aggaggaggt ccgagggatc 300
ctgggaccag gtagagcttg ggcaggtgaa caggggctgg caggaatagg ttgaacctgg 360
agctcctgga tgaggaggag cttgtgcaga tgagaaggat ctggagggca gggagcagac 420
ttggctgctc ctgggagcag gtagggcttg ggccgctgag caggggctgg gagggcaggg 480
agcattcctc tgacctctgg gagcaggtag agctttgcag gtgaggaggg gctgggagag 540
cagggggcag tcttgggaac tcctgggacc gggtagagct tgggcaggtc agcaggggct 600
gagagggcag gggaatgaac ctggacctct tgggagcagg tagagcttgt gcaggtgagc 660
aggggtgggt aggacagggg gcagtcctgg cagctcttgg gagcaggtag ggcttgggca 720
ggtaagcaga gtcaggaggg caggggatag acctggcagc tcactggagc aggtagagtt 780
tctgcaggtg agcagggcct aggagggcag ggagcactcc tgggagtgcc tgggagcagg 840
tagaaattgg gcaggtgagc atgggctggg atgcaggggt tgaacgtgga actcctggga 900
gcaggagggg cttgggcagg tgagcagggt ctaggagttc agggggcagt cctgggagct 960
tccgcgagtt ggtcaatctc gggcaggtaa gcagggactc agaggcctgg agcagccctg 1020
ggaccatctg ggagcagggc gagcttgggc aggtgagccg ggccaggggg gatgcaggac 1080
agggcagggg caacagagtg ggggtgagct caggggagcc ccaggctcga gggaggggcc 1140
gatatagggc tagccaggct ggaaagtggg ctcctggggg gaaggtctcc aggactgcgg 1200
gggcaggggc agggctgaac agacgccatg ggtcacaggg ctcgggccca cgggccccag 1260
ccctggtcga gcaaagtgag gggacatagg acagagcagc tggagtcctg agtgagaact 1320
gtggcacagg gcagtggacc tggtcggagg gtctaagggg cccttctggg actgagctgg 1380
agctgcaggc aatggctgag cagacggggt gtgggggggg taatggccca gccgggtagg 1440
ggcgcctggg gtgctggcgc cccgcgggag aggaaggcca ggggcagggc tcggctgcag 1500
cagtgctgcc aggcaggctg gcagcgggaa gggcaggagc agagggaggg ctctggcctc 1560
agcaggcagc tgggaggccc agagctgggc tgcaggggcc ggggctctgg ggaaagctgc 1620
ctcgggtgag ctcagggcca agccaggagc cccggtgcag gagggaccgg tgggcccaga 1680
ggcccagatt tggagcctgg gccagggccg gagggatggc aggggagggg cctcggagga 1740
gactacctgc cctcgcccag ggaaaggggc atgttggtgc cagggaccaa cgcgcacggg 1800
ccttgactgc agagctgacc cagaggaacg gggcctggag agcaaacagc aggccaggac 1860
caggggccag gagcagagca tgggcagtgg gtggcagtgg gtggcagtgt ccctgccagg 1920
ctggggaagc aggacggtgg cctcagggga ccagcagaag ctggccagga acacaggcca 1980
cccgctggtc gggccaggca cccacagggg ctctgggccc tggcgggctc tcatccaggc 2040
ggtcacccag ctctgaccag gcagctgcgc ccagaacctg cagctgcaat ggcagcgagc 2100
tgggcggctg gtctgccgac tttctggaag caagtgggtg ctgggcgcag cggccccgct 2160
cttctcgggg cccgattcgc tgcccgcccc acaaggaaca aggccctggc ggtcacgcag 2220
cctcctctct tccagcctac aacacagctc catcggtcta ccctctggcc ccctgtggca 2280
gggacgtgtc tgatcataac gtggccttgg gctgcctggt ctcaagctac ttccccgagc 2340
cagtgaccgt gacctggaac tcgggtgccc tgtccagagt cgtgcatacc ttcccatctg 2400
tcctgcagcc gtcagggctc tactccctca gcagcatggt gatcgtggcg gccagcagcc 2460
tgtccaccct gagctacacg tgcaacgtct accacccggc caccaacacc aaggtggaca 2520
agcgtgttgg tgagcgcctg cgctgggggc gggagtgcac gtcaagacag gccggggtga 2580
gccccctgcc cgcagggacc agttcccagg atggagagtc ccacccaggg tgtccccctc 2640
acctgcaggc ccaggctcag ggaggggtca actcggcact tcggagaggc cagggtgggc 2700
acaggcggga cccctccccc ggccctgggc ccccagcagc acgtgatgct ctggcatgta 2760
ccaagacctg accctgacct gagcccagcc ctgggccctc cccaccccca gtgaccctgt 2820
gtgttctctc tgcagacatc gaacccccca cacccatctg tcccgaaatt tgctcatgcc 2880
caggtgagtc agtcgggcct gaccctcctc cctgaagagg tggcccgagc tgggtctcaa 2940
cacgagggtg cacgggtgcc caggagcacc ccaggccagg tgctgacccc acactgtgtc 3000
tcctccacca gctgcagagg tcctgggagc accgtcggtc ttcctcttcc ctccaaaacc 3060
caaggacatc ctcatgatct cccggacacc caaggtcacg tgcgtggtgg tggacgtgag 3120
ccaggaggag gctgaagtcc agttctcctg gtacgtggac ggcgtacagt tgtacacggc 3180
ccagacgagg ccaatggagg agcagttcaa cagcacctac cgcgtggtca gcgtcctgcc 3240
catccagcac caggactggc tgaaggggaa ggagttcaag tgcaaggtca acaacaaaga 3300
cctcctttcc cccatcacga ggaccatctc caaggctaca ggtgcatggg gtgagaggca 3360
gggagggtcc cgtggggcca cctgggggga ccatcatgct aacagaggtg tctggcctca 3420
cagggccgag ccgggtgccg caggtgtaca ccctgccccc agcctgggaa gagctgtcca 3480
agagcaaagt cagcataacc tgcctggtca ctggcttcta cccacctgac atcgatgtcg 3540
agtggcagag caacggacaa caagagccag agggcaatta ccgcaccacc ccgccccagc 3600
aggacgtgga tgggacctac ttcctgtaca gcaagctcgc ggtggacaag gtcaggtggc 3660
agcgtggaga cctattccag tgtgcggtga tgcacgaggc tctgcacaac cactacaccc 3720
agaagtccgt ctcccagact ccgggtaaat gagccactcg ctgcacccca cgtgctctcg 3780
ggtcccaaga gctcacctga gccccagcgc tgtgtacata cgtcccgggc cagcatgaaa 3840
taaagcaccc aacaccaccc tggcacccgc aacgctgtca tggttctttc cgaggcagag 3900
ccccagtgca ggcgccaccc gcggggccag ggacaggaca ggctctgggc tagaggcgtc 3960
cccgtggcgt cggggggaga ggtcccacct gggccagacc cggtcggtgg gggtctgaac 4020
aggccccctg ggggctgccc gaggccgagg agggagcttt gcctgaggcg gcctctcctg 4080
cgggctgccc tcctggcaca cctgctccgg ctgctgtgtg tggacggacg ggtgaagccc 4140
tccaggcccc ctcggggaaa tgactgccca ggtcgtcccg cctctgtcca agcccaagct 4200
caggggcagc ggcccgtcct tgtccccagg ttgcctgtct cctgggtctc ctgtgcccac 4260
agggtgggga cggccctgct cctcccgcaa acacccgaca tcaggccccc accccgcctc 4320
aggccacggg gctctgccct gctggtcccc gcagggggcc aggcctgggc actcgtggca 4380
acactcaccc ctgtgtccca caggggcaga ggtaggcagc ccagagtgtc caggggaggg 4440
acagtcccag acacccccag gagtggagcc cagacccact gatatcaagc ccctcccacc 4500
gagctgccca gcccccccca ccccaccgcc ttcctccact cgcacacccg ggctctcgca 4560
cacgcatgca cgccgccccc acctcccagc acacccgccc accgcctgca tccacatgca 4620
cacacacccg ggcacacaca cacctaccca cgcacacatg tgtacacccc cgggccctca 4680
cagaccagag catggagctg ggtctcacac accctcaccg gggacaccag cccggcccca 4740
tcccccgagg ccccacgggc tgctcagcca cactggcaca ctagaccaca cggacccgct 4800
ccgacgcccg gccccgctct tcccctggca cctacgcccc ctccggaaca agcagaaatc 4860
cccagcccgc ttgtccccag cacagccgcc cccgcggccc ggggacgcac accccaaggc 4920
cgtctcggcc cttccctggg ccagagccca ccgggcccct gctcggctta acgcccaccc 4980
tccgggcctg gccccctcgg agagagggcg gggcctgagc cactggcctg cacggtcagg 5040
acaccccctc ccagacggtc cccatgctcg ggggccccca ccagaggaga gggggcagga 5100
catccctggc caggctgaga ctcagcccag gatgcaggac tggggggccg gggccggggc 5160
caggcactga gctcagccgg ggggtccccc acctggagcc accccaggcc ccgcgggctg 5220
gcacagcact gaccacccct ccccgtgcag aactgctcct ggaggagagc tgtgccgacg 5280
cccaggacgg ggagctggac gggctctgga ccaccatctc catcttcatc acgctcttcc 5340
tgctcagcgt gtgctacagc gccaccgtga cgctcttcaa ggtgagcggg ccagccccgc 5400
tgtcacccgt gccccggagc cccctgccgg cccctccctg gccctccctg tggccacgct 5460
gtcactgtga cggcccctcc ctgtacccgc cctggcctct ggtcctctcc gggacacggt 5520
ggctgtcggg gaccgcgggg ctggcaggcg gaccctgggg ggcggtccac gtgcggcccg 5580
ggccgccctc accccgtgcg ccctgcaggt gaagtggatc ttctcgccgg tggtggagct 5640
gaagcggacc attgtccccg actacagaaa catgatcggg cagggggcct agggcgccct 5700
ccagggcggc cagggccgcc ccagacccga caggaccgcg tccgtcggct cctgctgctc 5760
cgcctgcgcc aggcctgcct ccgccctctg acctcgccca cctctggctc tcggccacct 5820
cgcccgcacc tctgcccttg ggcatcgcgg ccccctcaga aggcgccccg cacccccacc 5880
gcccctcgga agcccgagtg ggcagcacct cgggccccga cctgacttga tggacaccca 5940
ccaccaccag cccccgccag agccacggga taccgccggg gccacgtgtc ctcaccagga 6000
acgcccggct cagccagcag gtccagccgg ccacgaggag accggcgtcc agcggctgac 6060
gggacagggc ccgggctccc tgtggacccc gtccagaccg ggctccagag gcagagtccc 6120
cgagaggagg ctcctgtgtc acagcctgga gtgggaacag ctgcccccaa cgccagcatc 6180
tcccagcagg gaagttccca gacgggccac caacaggcgc tcaggtcacc tccagcccag 6240
ggcactcgag ggaggaggac ctgggcccga ggccacggca ggagccccgg tggggacaga 6300
gcggccctgc ccagggaggc cgtgaggtgc cgccagaccg gcctcctgga gggggtcccg 6360
ctgactcgga cccccgcagg caggatgggc cctcactgga tgcaggcggg ggtggagggg 6420
ccacccaggg tacagggaag ccagcccacc ccgaggtccg cggcctgagt cacgtctatc 6480
ggaccatccg tccatccagc aggagatcct tctgcctgcc ccggggcctg ggtgagaggt 6540
gaagaacccc caggagcgag ccccaggccc cggaccccca aggccgcccc agaacggact 6600
ctcccgccct gtctctgcgg gaaccccaag tgggttcacg gcatccgggg agcagcctcg 6660
ggactgggag ggaaggctcg tctgccgagc tgtggtcgct ctttctcctt ggtgagtgtc 6720
tggagcccag gcactctcct cactgttgct ctcatttttt acagcaataa accactttta 6780
caacccctca gctcactggg acctctgcct gtgtcacccg cctccccccc gaatcccagc 6840
ctggcgtcct cccacgacct ccgcccctgg catcccccca tgaattcctc ccccaccccc 6900
aagcctcctg ggcacagcgg ccagcctggg gcctggcggg gccacgagcg actcgcatgt 6960
gttggctgtg tcatcagcct gtgctgctgt cgcaaacacc gcagccagtg gggtcgccgg 7020
acaaactgca ccaactcaga gctgagactt aggtcttcct ggggtgacat gaggccgcag 7080
ccggggaggc agcactgcag ggagctcgcc ggtaccgctc gctccaagca ggaggggact 7140
gaatgtcacc atgtgtgtga caggagggag ggcctgggaa ggccaggcca gggggtatcg 7200
ggggacagag gtcgggagaa gggctggccc agggagcaga gctggacccg gctcaagcgc 7260
tcaggacaga cgggtcaggg agaaggacgc ggggggcggg gaaggggacc gtgggcgcag 7320
acctccgcct gggctgccca gagggcctac ccatcccagg acaagccgag gggccgcacg 7380
gggctccggt gagggaccag ggaaggggcc tggtggttgc gaccaggccg ccgtgtccgc 7440
cggccggctg acagccgtcc agggtgcggc caggactcca acctcccaga ggtgatcccg 7500
ccccagagga acaggcctcg ggccggggct ggaggggaca ggactctccc aaaagggcag 7560
agcagggggc ccggctgccc gtgcttccta gtcacagctc caggaagggg gacagggcca 7620
gcccggggct gtcccaggga ggcatttgac gggagccggg tcacctgggg acatgacctg 7680
ggccactgaa tgctggcgct gaaagcctct cgaggcgagg ggaggagccc caggccgagg 7740
ccagcaccac gtttcccagg ccaggagctg tggtcctgct cagcctggcc cagagcccag 7800
ggcatcggca agggcccctc cagcgtcccg cagggaacag gatggagacg gagccagagc 7860
agcatgcagg gaacggactg cgtcgctcca ggggtccctg aaccagacac ggggaggccg 7920
ggacgtgcgg ccagagcatg gacgtggggt ggacagcaga ggccaggcat gcagggcacg 7980
gagcgccctc cgcctccagg ttctgggggc tcaggccacg gtcctggggc tcaccccaca 8040
ccctccgttt ccctcaccct aaacgtctag ccaaacctga acgtgcccat gggccacgca 8100
gcctgctcag ccccaggccc tggccctcac gtctgtcccc gcaccagcca tcagcccctg 8160
gccggcccac tcccgcggga ccgccagcat ggcgacagcg gggctggccc ccgcaccctc 8220
gagaactccc ccaccggacc caggcctccc accctgaccc ccagcaacgc ctcatcccac 8280
ggcaacccca cctctccacg tgccccgcgc accccgagcc cagggcccga cgccagcccc 8340
tgagcccctc gggctcccct gagtgagcct gaagccagac ccccgcggcc tcctgacccc 8400
tagaccccca gcattagctt tacacacacc ggtaccatct caccctgccc acgcacaccc 8460
acatctgcgt cgtacacaca cacacacaca cacccccacg ggcacatgga cgtccaggat 8520
ttccagctca gccccaaagc cgagggcact aaggggcccc tccgatcaca gcccccacgc 8580
ccccccaact tggggccact cccggaagag cgtggctccc ccgcagagag aaccccgctg 8640
gacgtgggtg ccgaacaccc attcgcactg aaccaccctc gaaacagagc gcgggcccac 8700
ggtccagccc acactcagag cccacagcca aagcgagggc tcagaggcca aggccggccc 8760
acggtccccg tgcaccctgc tccggagtcc gaagccagcc cagccccgcc aggcctccct 8820
ccccaggctc agcccgcccc tccctgcccc cagctcccag tgcccgtgcc ccccgtgccc 8880
agcccagtcc ctgtgcccac gcccacccct gcggtccacc ctccctgctg gccaccccag 8940
gccccaggcc tgcagaatga ggaggcagag aagccttctg gggagaggcc gtgggaccct 9000
ggggctgcgg gcaaggccct cggggtggga gggggccctc acggaaggag cttcccgggg 9060
ggtccacgcg gccaccgtct cggcccagct gccccacccc cactcaccgt ggcctcgccg 9120
gggcctcgct cctttcttag caaagctcct tctccctgag gaaactgtgc aagtggggga 9180
ggctgccctg ggtatcaggg cggctggggt gggggcactg gggatctgtg tgtgtgtcgg 9240
ggggtgcact gtggctctgc actgacggag gtgggatgaa caggggattt accggagtgg 9300
ggtgcactgg ggatctgtgc aggtgcgatg ggattcccag ggaatcggtt caggagcagg 9360
tggctttgcc ctggggatct gtttgcatgg ggtggggtgc actggtattc tgtgcagaag 9420
acatggcgtg cactagggat tcgtgccggt ggggttgggg tgcactgggg atctgcgcag 9480
gtgggggtgg ggtgagctgg ccggctctgc aggtggggtg gggtacactg gggatctggg 9540
cagttgcggc ggggtgcagt ggggatctgc gcaggtgttg gagtgcactg aggtctgtag 9600
ggttgggggg cactgagtag ctgtgcaagt ggggtgggag atgcaccggg accggaagtg 9660
agggggtggg gtgggctggg atgggagcag gtgggtgggg tgccctgggg atcaggtagg 9720
gggccttcgt gatcactggc attggcaggg cagggggtcc gggctctggc gagtgaatct 9780
gtacctagac gaaggccaaa gcacacgtaa atacatccac acctctgact ctcagctgca 9840
gccgcccgcc caggacacac gaccaccatc cgcaggaacc agcccaggcg gcggctgcgc 9900
cctctgcctc agacctgcct ctgtctacag ggacagcccg ccggacagga ggtgccctgg 9960
ccaccacacg gggccgagcc gcgctggccg acccgctgcc cctgccccac ggcctccatc 10020
accaccccga cacgccctgt gggaaggagc cctctgcacg cgggaatcct ttgaaaggtc 10080
ggcgtacgca tcacacaggg ggttcacctg tactgcctgt gcgcctaatg ggccctgcta 10140
tgcacacgcc accttcacag taagagtgaa cactcccagg cgtcggagac ccacggggcg 10200
gccccgccag cgggtggggg ccgctccctg ggggctcaga acacacggcc tcggctttgc 10260
ccctggaaac acgtccagtg gagcaggaga cacaggtgtc cggcaacggc tcggggccgt 10320
ggttgcaaac ggcgcccgat gcatcctggg acctggaccc aaggaggggg cgagggggaa 10380
gctggccaag ccgcctgggc agggagggga tggctcccgg aggcggaggt gagcagagaa 10440
gggggcggcg gggccggccc ggggcctgag cagcgccgcg ggcggggagc gtcctgctgg 10500
gaccaccgct ctgccctggg cccgccctcc tcgtcctcac aggactgacc tcaagccagg 10560
gctgcccgca cctgaagggc acgcagacca ccccaggagg agactccggg cctttctctt 10620
tttcgttttt taatttcatt tgactttatt ctatattatt ttatattata ccgttttagg 10680
gccacaccca cggcatttgg aaggtcccag cctaggtgct gaaccagagc ggcagcagcc 10740
gcctgcacca cagccacagc aacaccagag ccgagcagcc tctgtgacct acaccgcagc 10800
tcgcggcgac gcgtcctcat ggacgccagt cggattcatt cgcgccgcgc catgacgcaa 10860
actcctcttt cgcaatttct tccattgaag ggtagtttta caccgtcgta aatttgccat 10920
gttgtgttac tttcaggtcc ccggcacagc ggcccggcca cacacacaca tatgcatcca 10980
cacccacgtg cagacaggca gacacacgtc aggacacata cacagcggtg tgtggagagg 11040
ttgatcccaa cctcctggct catctcccca cctctcgccc tggggaagcg tgcggtggcc 11100
ccatgtctgc gggtccgtcg cggttttgct tcgagtccac tcgcacccct ccttttaatc 11160
ccacacgcag gcaatgccgc acgatgtcgg tctccgtccg cgtcgcttca gccagaggag 11220
caggacgacc tctaggtccg acctcgtggt ggcaaatggc atcgccgcat cctttgcaca 11280
gctgagcgat aacccgttgt gcctgtgccc ctcaccttct gcaaccgtcc gtccagacgg 11340
acgtttgggc cgcttcctgt cttggtcatt gcgaggagtg ctgcgacgga tgctggagag 11400
cccgtgcgtt ttcacgttgt ggttctctcc cactgtgtgc ccagcactgg ggttgccgca 11460
tcggacggca gttctgttcg cagtgctttc aggaagctca ggccgctttc cggagtggtc 11520
gtacctacgt ccccaccgac agcgtagggg gttccctttt ctccacaccc tctccagtat 11580
atacatcccg tttgcagact ttttgatgat ggccactctg gctggattga ggcgctacct 11640
catggcagtt ctgatttgcc tttctctaat aattagcaac agtgggcacc ttttcatgcg 11700
tttttggcca actgcacgtc tgtcttcttt ggagaaatgc ctgtgtaggt cttttgcctc 11760
tttcttgatg ggattcttca taatcaagct gcatgccttg tttgtatgtt ttggagatga 11820
atcccttggc ggtcccttca ttggcaaata atttctccct ttttgtaggt tgtctctttg 11880
ttttgcttgt ggtttccttt gcagtgccgc atatttttaa gtttaatcag ttcccgtttg 11940
tttaattttc tttgtatttc cattgatcta gaagacgtgt ccaaagagat attgcgatgg 12000
ttcatgtcaa agtgtatcct gcctattgtc ctccaggact tacacatatc tggtgctaca 12060
tttacatctt ttaatcattt tgaggggttt gtgcgtgtgt gtgtgtacac cgttggagaa 12120
tgttctactt ttgctatttt acaagtagct gtccggcttt cccagcacca ttgattgaag 12180
aggctgtctc tactctattg aatattcttg tttctttgtc atggacgaat tgactgtaag 12240
tgcttgtgtt tatttctggg ttttctatcc agttccgctg atgtgtgtct ctgtttctgt 12300
gacagtgcca tactgttttc atgactgcag ctttgtagta tagtcagaag tcagagatcc 12360
tgattccacc agttccattt ctcatactca acatgctttg gctatttgag gtattttgtg 12420
tttccataca aatttaaatt ttttctgttc cagttctgtg caaaacgcca ctggcaattt 12480
gatagggatg gcgttggatg tgtagattgc atggttactg cggtcatttg gacaatgctg 12540
agtcttccaa tccaggagca tggtctggct ttccatctgt ttgtgtcatc tttgattgct 12600
ttcatcagcg tcttctagtc ttcatagtac gggtcttttg cctctttagg taggtttgtt 12660
cataggtctt ttattctttt ggatgcattg ataagtttga ttatccccca cccccccgcc 12720
ctttctggcc ttttgttgcg agtgtgtaga catgcagtag atttctgggc attagtttgt 12780
atcctgccac ggtacctaat gcactgatga gctctagcag ttccctggtg gtatctttag 12840
gacttcctac gtgcagcgcc atgtcctctg caaacagtga gagtcttacg tttctcttcc 12900
aaaaggacgg cgttagtttc tttttcttct ctgactgctg gggctaggac ttccactacc 12960
atgttgagca cacggcggcc aactatgtgg tgagggtggc catcctagtc ttggtcctga 13020
tcttagtgga aatgagttca gcttttcacc ttggagtgta ttaacggcgg gctcctccta 13080
tggactgtgt tctgttgagg ggcattctct gagaccctga tgcccccacg cacaagtccg 13140
aggagccctc tgaggcagaa gaccctgggc ttcccggggg cccccgagcc tcgacgacct 13200
tctcccctgt gggcatgctg ccagcccgcg tgccgatttt atacaccttt gatttcctgg 13260
gtggcatcac ttctcacttg aaacccctag aggggcttat gtttcctgga ttcgccctgg 13320
actggcacgg caacaagtga aatgtcctag caatacaatc tgatctcatg aaaagagata 13380
aaactcaaaa taaacaatgt gaacttacac ctaaacatct ggaaaaggaa aaagagacaa 13440
cacccaatat tagtagagga aagaaatcac aaaaatcaga gcagaaatca atggcataga 13500
gatgaagaaa atgatacaga agatcaatta aatcaaaatg tggttccttg aagacgtcaa 13560
caaaatcgcc aaatctttag ccagcttcct caagaaaaaa agggagagag ttcacgtcta 13620
taaaattaga aatgaaaaag aagttacaac tgacaccaca gaaatacaaa ggaccaatat 13680
cagttcctcc aatccaggag catgggctgt atttccattt ctttgaatcc tctttaattt 13740
ccttgataat gttttatatt tctcagcaaa taggtatttc acctccttag tcaggtttat 13800
tctgaggtat gtaatttggg gcctgctatt ttaaaagatg tcatagtttt ctatttcttt 13860
ggtaacactt tattgttcct atacagaaac gcaaccaatt tctgaatgtt gctcttgtat 13920
cctgctactt tgctaaattc attgatcagt tcaagcagtt tatgagtgga gaccttaggg 13980
atttctctgt acagtgtcat gtcatctgca cagaatgaca atttcacctc ttcgcttcca 14040
tcttggatac cttttatttc ttctgtttgt ccctttgctg aggctaggac ctccaatact 14100
atgttgaata gaagtggtga gactgggcat cgttgccttg ttcgtaaaag acagcaatgg 14160
tggtaaggct ttcggctttt ctctgttagg attaaagtgg ctgtgggttt gtgacaaatg 14220
gctttaatct tcgacaaagg aggcaaggat ataaaatggg gaaaagacag tcttttcagc 14280
aagtgttgct agctaaaacg gacagccgca tgtacatcaa tgaaactaga acacaccctc 14340
acaacaggca cgcacacaca cacacacaca cacacacaca cacacactct atatggctta 14400
aagacttaaa caagacataa caaaactctt agaagagaac acaggcaaaa cattttctgg 14460
catcaaccca cacaaatgat ttcttaggtc actctcccaa ggcaacagaa acaaaaccaa 14520
aaataaaccg atgggatgta atcaaacgta caagcttttg cacagccaag gaagccgtta 14580
aagaaaggac aacctacgga ataagagaaa agagtttctg acaatgcaac cgacaagggc 14640
ttagtctctg aaatatacaa acagcctaca caactcaaca gcaagaaaca ccacccaact 14700
gaaagacggg cagaagacct gaatagacat gtcgccaaag aagacaaaga gatggccaac 14760
gggcacacta aaaagtgctc aacatcactg ttcattagag aaacgcgact caaaactgct 14820
atgaggtacc aactcacacc tctcagaatg gccatcatga aaaagcccac aaataacaaa 14880
tgatggaagg ggtgtggagg caagggaacc ctcctgcact gctggtggga atgtaaactg 14940
gtacaaccac tccggaaagc agtatggagg tacctcagga aactaaatgt agaactacca 15000
tatgacccag caatcccact cctggggata tatccacaca aaactttccc tgaaaaagac 15060
acatgcaccc atgtgtgttc actggagcac tagtcacgct cgccaagaca tggaaacaac 15120
ctaaatgtcc atcgacaggg gaatggatta agaagatgtg gtatatatac acactagaat 15180
accactcggc cgtaaaaacg aaccaaataa tgccattggc agcaacacgg atggaaccag 15240
agactctcat actaagtgaa gtaagtcaga aagaccaata ccatacgcta tcacgtacgt 15300
ctggaatcta atatatggca caaataaacc tttccacaga aaagaaaccc atggacttgg 15360
agaaaatact tgtggtcgcc aagggtgagg tagtggggta gactgggagt ttggggttag 15420
tagatgcaac atattgcatt tggcatagat aaactatgag atcctgctgg agagcacagg 15480
gagctgtatc tagtcccttg tgatggaaca tgttggagga taaagtgaga aaaagaacat 15540
ataaatatat acaccacacg cacacacaca cacatatatg aatgactggg tcactttgcc 15600
gtagagtaga aattgacaga acacagtaaa tcaactataa cggaaaaaaa taaaaaacat 15660
taaaaaaaaa aaaacagatg ctcgtctcca tggattctga acacattttt gacagaattc 15720
aacacccaac tttttcttga tgatttttat tttttccact gcaggtggtt taccctgctc 15780
tgttgattcc actgtacaga aaagtgacaa agtcacacac atatatatat gtagacattc 15840
tttttctcac attatcctcc atcatgcccc atcataggtg actagataga gggaaactac 15900
accaacatac taaaagccat atatgacaaa cccacaacta tcatcattct caatggtgaa 15960
aacctgaaag catttccgct aagatcagga acaagacaag gatgtctgct ctcaccactc 16020
tccttccacg gagttttgga agtcctagcc agggcaatta gagaagaaaa ggcatccaaa 16080
tcggaaacgg agaagtaaca ctaccgctct ttacaggtga tgtgctgcga tacctagaga 16140
atcctcaagg cactaccaga aacctcttag agctcgccaa tgaatttggt caagctgcag 16200
gatacaaaat taacacacag aaattggctg ccttgctcta cgttaacaat gaaagagcag 16260
cgagagaaac taaggaaacc atcccactga gcagtgcctc gaaatgaatg aaatgcccag 16320
gaataaacct acccaaagac acaaagacct ggactctgaa aaccataagg cactgctgga 16380
agcaaccaaa gacgacacaa accggtggaa agctagacca tgctcctgcc ctggaagaat 16440
taatactggc aaaagggcca taccacctaa ggccaccctc actctcagtg caatccctat 16500
caaatctcca atggcatttt ccacagaact aaaagaaaaa gggttttaat ttgcatggaa 16560
acacaaatac ctcactgacc tgggctatac tacaaagctg cagtcatcaa aacggtatgg 16620
cactgacact cccccccaaa aaagacatac atatcagtgg aacagaacag aaagcccagc 16680
aataaaccca agcacctatg ctcaatccat ccatgacata ggaggcaaga ctattcaaca 16740
gagaagggag agtcacttca ctaagtggtg ctggggagct ggacagctcc acgtaaagaa 16800
tgaaaccaga actctcccga acgccaaaca caccaaaaaa aataaataaa taaaaaaata 16860
aaaaaaccct caaaacaaat gaaactccta aacgtaagac tggataccta taactcacag 16920
aggaaaacag gcagaacgtt ctttgacata aatcacagca acatcttatt tgatccacct 16980
cctggaataa ctacaataaa gacaaaagtg gagttcccgt cacggctcag tggtaaccaa 17040
atctgactag gaaccatgag gttgcgggtt caatccctgg ccttgatcag tgggttaagg 17100
atccggcgtt gccatcagct gtggtgtagg ttgcagacgc ggctcggatc ccacgttgct 17160
gtggctctgg tgtaggccga cagctacagc tccaattcga cccctagcct gggaacctcc 17220
acatgccatg ggtgcggccc tggaaaagac aaaaagacgg ggaaaaaatg aaagagagaa 17280
agaaggaaag aaggaaagga ggaaagaagg aaaggaggaa gaaagaaaga aagaaagaaa 17340
gaaagaaaga cagacagaca gacagacaaa agtaaaccaa tgagatctaa ttaaactcaa 17400
aagcttttga aaagcaaaag aaaccatttt aaaagagaaa aacaggagtt cccgtcctgg 17460
ctcaggggtt aacgaatctg actagcatcc atgaggaggc agatttgatc cctggcctca 17520
ctcagcaggt taaggatccg gcattgccat gagctgtgag ctgtggtgta ggccagcagc 17580
cgtagctcca attcaacccc tagcccggga acctccatat gccacaggtg gggccctaaa 17640
aaccaaaaaa gacaaaggaa aacaacccac agaatgggac aaaatctttg caaacaatgc 17700
agcccaccaa ggctcaatcc ccaaaatata caaacactct acaactcaac aacaacaaaa 17760
aacaaaccaa caaacaaccc aatcgataaa tgggcagaag acctaaatag acatttctcc 17820
aaagaagaca tacagatggc cagaagacac atgaaaaaat cctcaacagc actaattttt 17880
agggaaatgc aaatcaaaac tgcaatgagg caccacctca cgctggtcgg aatggccatc 17940
attaagagtc agctaacagc aaatgctgga gagggtgagg agaagaggga ccccgccttc 18000
actgttggtg ggaatgcaac ttggtacaag cactgtggaa aacagtatgg aggaacctca 18060
gaaaactgaa cgtaaaacca cgattgaacc cagaaatctc acccctcggc ctgtatctcg 18120
atacgacttt cagtcaaaaa cttacatgca caactatgga ctttgcagaa gggttcaaaa 18180
tgggcaggac atgaaaacac tcaaacgccc atcaacagac gaacggatta agatgtggtt 18240
ctcacacaaa gtaagaacta cgtctcagcc gtaaaaaagg accaagtcac gccatttgca 18300
gcaacgtgga tggaactaga gactctcaca ctgagtgaag taagtcagaa agagaaagac 18360
agagaccaca tgacaccact tatatgtgga atctaaaata cggcgcgatt gatactgcgg 18420
acaaaacaga gacagagcat ggtcatggag agcagccttt gctttccagg gggagggaga 18480
gaagagattg acagggggtt tggagctggt agatgcaact ggtcacattg aaaacgagta 18540
agtgatgggt cctactgggc agcacacgga acagtgcccg gcctcttgcg ttagaacgtg 18600
agagaggaag gagagtgtga aaagaaaaag aaggtaaatg tatgtacagc tgtacaaggg 18660
taattgaagg aacgttgtaa atcaactctg gttttaaaaa aaaaaaaaaa aaatttaaag 18720
aaagaaaacg ttctccagag gcagaacgag aaggtggaac ggccgacagg tctctgtcct 18780
gcagcggccg ctgacacgac cccaggacag cggaaatgag acccaggctg cctgtcgggc 18840
acacggcggc cgccccggca ccctggtgat cctgtgcagc acgaggtgcc cagtagaggg 18900
gtgggcaggg cagggcaggg cagggcagga cctggggacg cggccgcagc aggtgggctc 18960
cagccaggaa ggagccacga gtggggtggg gtctgctcgc tgggctggag gcagggaggg 19020
cacctcggct ccaacatccc agccgggcac ccggccagag gaaggggctg cgaggatgtc 19080
ttccaagcat ctctttgctc ttggaaccac gtggcgaagc tttctgaaag cagaccagac 19140
tgcagcacca cggtttctat tgtgaaggga tttttccaga aggagtggga ccttgcgaac 19200
ggggatgtgg acacagggtg gcttctgatc gagcccaggg ccctgtgcag ccaagtctca 19260
tggtcacgcc gtcccggccg gaccagcctg ccccccacgc ggacccgggg aaggcagggg 19320
tcccatgacc agcacgagaa cgactgactc gccttgacct caagccacct ccaggaccag 19380
gggcagacgg cccggcacca cgaggaggag gcagagcccc gggcagggcc cacttgccac 19440
ttcacctcgc cagagagccg ggcagatggc ggcacgcgac tcttggggcc tgacggcccc 19500
gagcccagac gctctcgggg ctttggccag actcgcaggg ctgctgagcc aagcagaggc 19560
tacaaagctc cacgggcttc cagggcaacc gctagagaca cgtgggcaca gctcgcagag 19620
ccggtgaccc acgggccaga tgggaacctc cctcctgtgc cccctcaagg cgtctcccgg 19680
aacggtggcc tcccacggcg ctggacgggg ctcggggcag gcccgaggca gcacagcgcc 19740
cacaccgtcc actggccgct tggaaggatt cagccaacac caccccaggc ccgctgcaca 19800
gccggggtgg gggctgagac gtccctcagg ggtccccagt cccagcctga tgctgaagtt 19860
acaggacggc ccaggggcgc ccaggggaca gggggagctg cggccctcca cgaatgagga 19920
cagagggtcc ctgaccccca cgcacggccg gcaggtctcg gtccacgcag agcagtgagc 19980
ggctgcccgc cagcccgctc tcggccgggc accaccttca caacccctgg gaactctgtc 20040
cccacgtggg cagcacgggc ctgggtctgg gcagcaggct gggcccagcc acacacgcgg 20100
gggtcacaag aggtgcccgg tggggtcggg caaccaaggg ctcacactag gggcctggca 20160
cccgggctgc agtgggccag gcggcataat ccccgtggga catagagaac cacgacgtcc 20220
tgagttcccg caccctcccc accgctcacc caccccacac acccacatga gccgccaggg 20280
cactgtctgg actccccagg ccctctggga cagaaagcaa ccagaagaaa agggaacttc 20340
aggaagcagc cgggccaccc ggtttcaatc ccattcttag tgtgcagggc tctgggcagg 20400
ggggtcgtgc ctccagggac cagacaccca cctgaggaag cagggctgca ggcacagagc 20460
acagacacaa ggagccactt gcacctgggg ccagagcagg aggggcccag agcccggggc 20520
agcaggggag ccacctcctc ggacgctaca ctcggtgcgt ggggtccacg aggactgggg 20580
caggggcagc ggcgggggga cgaggagggg ggaccccaca gcagggctgc agccgaccca 20640
gcccagggat cgcagcctgc acgcgggtca aagcacctgc ccagcacagg gaccaggcag 20700
ccctggcagg ggcacaccca tgccaacaga gctggcgccc cagaggccag gggaggtcag 20760
tggggccgcc aggcctctgg ggaacatgat gggctggggt cggaggcctg agggatctgc 20820
tcggccaggt tgctggaggc acagggctgg gcaggggctg aggacgaggc caggatggac 20880
aggtgggtct gggcggagga cccagggaag agacggacac agcgcagggg acaaagaggt 20940
cacctggggt tgcagggtcc tgtcccggct gccggagccc gcttgagccc cagggcaggt 21000
atgaaagcag taactgtcgg gtcccgggga aggagccagg gccacgggca gcaggtgagc 21060
cgggagcaga gcaggcaggg cgggccgggc tcctgtgtgc ccagagctgg cagcacaggg 21120
actgagccta aagctccaca ggccgcagga gggctgacgg gagccagggg cctgagggca 21180
ggagcgggag ccaggggctg ggagctgggc gccagtgcag ctgagccagc aactagaggg 21240
agccagggac cagacaggct ccaagggtgg tcgcggcgag aagagggtga gctcagaagg 21300
gtcaggagcc cctccgggag gggaccaggt gggacagagg ctctgccagg gggacgctac 21360
ccagagaggg tgggcaccca gggctccggg aacaaaggcc agccctggga aagaaaggaa 21420
gccaggagtg gagctagaca aggctgaggg ttcgggggca ggaagcgggg tggagatgct 21480
gaggcccagg agccccaggg aaaggggcag gtaggctgca agtgagcagg gaaccgagaa 21540
ggcagaagaa ctgtccgtag agctgctggg aacacacaga gctggtgcag gtgagcaggg 21600
ggctgggagg gcaagggggc tgaacctggg acctcttgag agcagtagat cttgggcagg 21660
tgagcacgag ctggtaggac agggaattaa cctggagttc ctgggagcag ggggcagtct 21720
tgggagcccc cacgagatgg tacagctcag gcaggtgagc aggggctcgg agggcaggga 21780
gcagccctgg gacctcatgg gagaaggagg agcttgtgca ggtgagcagg ggctgggagg 21840
gtggggatca cctaggaact ccttggagca ggtagagttt gggctggtga gcagcagctt 21900
gcaaggcagt gggctaaacc tggagctatt gggaagaggt taggcttgtg caggtgagca 21960
gtgggtggga ctgcaggggg cagtcctgga agcccccgcg agctggaaga gctcgggcta 22020
gtaatcaggg gctcggaggg cagggagcag ccctggcact tcctgggggc aggtagagct 22080
tggacaggtg agcagggcca gggggaatgc aagacaaggc aagggcagca gcagagaggc 22140
tgagctcagg ggagtccagg ctggagggag gggccggcac agggttagcc atgctggaaa 22200
atgaacccct gggcgggagg tctccaggac tccagggtcg aggcagtcct gaacacacac 22260
gatgggccac agggctcggg gccacaggcc ccatcactgc tcaatcaaat tgagtggaca 22320
taggacagga cagctggagt cctaagtgag aacagcggaa agggcagtgc acatggtcgg 22380
ggggacgagg ggcccatctg ggcctgagct ggggatgcag gcaacggccc aacagaacag 22440
gggggagagg accggcccaa ccaggcaggg gtgcatgggg tgctggcgcc ccgcgggaaa 22500
agaaagccag gggcagggct cggctgcagc agtgctgcca ggcaggctgg cagcgggaag 22560
ggcaggagca gagggagggc tctggcctca gccggcagct gggaggccca gagctgggct 22620
gcaggggccg gggctctggg gaaagctgcc tcgggtgagc tcagggccaa gccaggagcc 22680
ccggtggagg agggaccggt gggcccagag gcccagattt ggagcctggg ccagggccgg 22740
agggatggca ggggaggggc ctcggaggag actacctgcc ctcgcccagg gaaaggggca 22800
tgttggtgcc agggaccaac gcgcacgggc cttgactgca gagctgaccc agaggaacgg 22860
ggcctggaga gcaaacagca ggccaggacc aggggccagg agcagagcat gggcagtggg 22920
tggcagtggg tggcagtggg tggcagtgtc cctgccaggc tggggaagca ggacggtggc 22980
ctcaggggac cagcagaagc tggccaggaa cacaggccac ccgctggtcg ggccaggcac 23040
ccacaggggc tctgggccct ggcaggctct catccaggcg gtcacccagc tctgaccagg 23100
cagctgcgcc cagaacctgc agctgcaatg gcagcgagct gggcggctgg tctgccgact 23160
ttctggaagc aagtgggtgc tgggcgcagc ggccccgctc ttctcggggc ccgattcgct 23220
gcccgcccca caaggaacaa ggccctggcg gtcacgcagc ctcctctctt ccagccccca 23280
agacggcccc atcggtctac cctctggccc cctgcggcag ggacgtgtct gatcataacg 23340
tggccttggg ctgcctggcc tcaagctact tccccgagcc agtgaccgtg acctggaact 23400
ggggcgccca gaccagtggc gtgcacacct tcccatccgt cctgcagccg tcagggctct 23460
actccctcag cagcacggtg accgtgccgg cccacagctt gtccagcaag tgcttcacgt 23520
gcaatgtcaa ccacccggcc accaccacca aggtggacct gtgtgttggt gagtgcctga 23580
ctgggggcgg gtgtgcagac gggctgaggt cagccttcct gccgagagac caccccccca 23640
gggtgtcagg gccgctcacc tctgtccccc tcacctgtac gcccaggctc agggaggggt 23700
caactcggca cttcggagag gccagggtgg gcacaggcgg gacccctccc ccggccctgg 23760
gcccccagca gcacgagctg ctctgggatg taccaagacc tgaccctgac ctgagcccag 23820
ccctgggccc tccccacccc cagtgactct gtgtgttctc tcatcaggaa aaaagaccaa 23880
gcctcgatgt cccatatgcc caggtaagaa actcgggcct caccctcctc cccaaggagg 23940
tagcctgagc ccaggttcgg cccgaggggg gacaggtgcc caggaggacc ccaggccaca 24000
agctgacccg cactctgtct cccccaccat gctgtgaagt ggccgggccc tcggtcttca 24060
tcttccctcc aaaacccaag gacatcctca tgatctcccg gacccccgag gtcacgtgcg 24120
tggtggtgga cgtcagcaag gagcacgccg aggtccagtt ctcctggtac gtggacggcg 24180
aagaggtgca cacggccgag acgagaccaa aggaggagca gttcaacagc acctaccgcg 24240
tggtcagcgt cctgcccatc cagcacgagg actggctgaa ggggaaggag ttcgagtgca 24300
aggtcaacaa cgaagacctc ccaggcccca tcacgaggac catctccaag gccaaaggtg 24360
ggcaaggtgg gcaggcggag agggtcccgt gcggccacct ggggggacca tcatgctaac 24420
tgaggtgcct ggcttcacag gggtggtacg gagcccggag gtgtacaccc tgcccccacc 24480
cgccgaggag ctgtccaaga gcatagtcac gctaacctgc ctggtcaaaa gcttcttccc 24540
gcctttcatc catgttgagt ggaaaatcaa cggaaaacca gagccagaga acgcataccg 24600
caccaccccg ccccaggagg acgaggacgg gacctacttc ctgtacagca agttctcggt 24660
ggaaaagttc aggtggcaca gtggaggcat ccactgtgcg gtgatgcacg aggctctgca 24720
caaccactac accgagaagt ccgtctccca gactccgggt aaatgagcca ctcgctgcac 24780
cccacgtgct ctcgggtccc acgagctcac ctgagcccca gccctgtgta catatctccc 24840
gggccagcat gaaataaagc acccaacgcc ctctggcacc cgcaatgctg tcatggttct 24900
ttccgaggca gagccccagt gcaggcgcca ccctcagtgt caggggtagg acaggctctg 24960
ggctagaggc gtccccgtgg catcgggggc agcggtccca cccaggccag acccgatcag 25020
tgggggtctg cacaggcacc ctgggggctg cccgaggccg aggagggagc tttgcctgag 25080
gcggcctctc ctgcgggctg ccctcctggc acacctgctc cggctgctgt gtgtggacgg 25140
acgggagaag ccctccaggc cccctcgggg aaatgactgc ccaggtcgtc ccgcctctgt 25200
ccaagcccaa gctcaggggc agcggcccgt ccttgtcccc aggttgcctg tctcctgggt 25260
ctcctgtgcc cacagggtgg ggacggccct gctcctcccg caaacacccg acatcaggcc 25320
cccaccccgc ctcaggccac ggggctctgc cctgctggtc cccgcagggg gccaggcctg 25380
ggcactcgtg gcaacactca cccctgtgtc ccacaggggc agaggtaggc agcccagagt 25440
gtccagggga gggacagtcc cagacacccc caggagtgga gcccagaccc actgatatca 25500
agcccctccc accgagctgc ccagcccccc ccaccccacc gccttcctcc actcgcacac 25560
ccgggctctc gcacacgcat gcacgccgcc cccacctccc agcacacccg cccaccgcct 25620
gcatccacat gcacacacac ccgggcacac acacacctac ccacgcacac atgtgtacac 25680
ccccgggccc tcacagacca gagcatggag ctgggtctca cacaccctca ccggggacac 25740
cagcccggcc ccatcccccg aggccccacg ggctgctcag ccacactggc acactagacc 25800
acacggaccc gctccgacgc ccggccccgc tcttcccctg gcacctacgc cccctccgga 25860
acaagcagaa atccccagcc cgcttgtccc cagcacagcc gcccccgcgg cccggggaca 25920
cacaccccaa ggccgtctcg gcccttccct gggccagagc ccaccgggcc cctgctcggc 25980
ttaacgccca ccctccgggc ctggccccct cggagagagg gcggggcctg agccactggc 26040
ctgcacggtc aggacacccc ctcccagacg gtccccatgc tcgggggccc ccaccagagg 26100
agagggggca ggacatccct ggccaggctg agactcagcc caggatgcag gactgggggg 26160
ccggggccgg ggccaggcac tgagctcagc cggggggtcc cccacctgga gccaccccag 26220
gccccgcggg ctggcacagc actgaccacc cctccccgtg cagaactgct cctggaggag 26280
agctgtgccg acgcccagga cggggagctg gacgggctct ggaccaccat ctccatcttc 26340
atcacgctct tcctgctcag cgtgtgctac agcgccaccg tgacgctctt caaggtgagc 26400
gggccagccc cgctgtcacc cgtgccccgg agccccctgc cggcccctcc ctggccctcc 26460
ctgtggccac gctgtcactg tgacggcccc tccctgtacc cgccctggcc tctggtcctc 26520
tccgggacac ggtggctgtc ggggaccgcg gggctggcag gcggaccctg gggggcggtc 26580
cacgtgcggc ccgggccgcc ctcaccccgt gcgccctgca ggtgaagtgg atcttctcgt 26640
cggtggtgga gctgaagcgg accattgtcc ccgactacag aaacatgatc gggcaggggg 26700
cctagggcgc cctccagggc ggccagggcc gccccagacc cgacaggacc gcgtccgtcg 26760
gctcctgctg ctccgcctgc gccaggcctg cctccgccct ctgacctcgc ccacctctgg 26820
ctctcggcca cctcgcccgc acctctgccc ttgggcatcg cggccccctc agaaggcgcc 26880
ccgcaccccc accgcccctc ggaagcccga gtgggcagca gctcgggccc cgacctgact 26940
tgatggacac ccaccaccac cagcccccgc cagagccacg ggacaccgcc agggccacgt 27000
gtcctcacca ggaacgcccg gctcagccag caggtccagc cgtccacgag gagaccggcg 27060
tccagcggct gacgggacag ggcccgggct ccctgtggac cccgtccaga ccgggctcca 27120
gaggcagggt ccccgagagg aggctcctgt gtcacagcct ggagtgggaa cagctgcccc 27180
caacgccagc atctcccagc agggaagttc ccagacgggc caccaacagg cgctcaggtc 27240
acctccagcc cagggcactc gagggaggag gacctgggcc caaggccacg gcaggagccc 27300
cggtggggac agagcggccc tgcccaggga ggcggtgagg tgccgccaga ccggcctcct 27360
ggagggggtc ccgctgactc ggacccccgc aggcaggatg ggccatcgct ggacacaggc 27420
tggggtggaa gggccacccg ggattcagag cagccagccc atcccgaggt ccacggcctg 27480
agtcacgtct atcggaccat ccgtccatcc agcaggagat ccttctgcct gccccggggc 27540
ctgggtgaga ggtgaagaac ccccaggagc gagccccagg ccccggaccc ccaaggccgc 27600
cccagaacag actctcccgc cctgtctctg cgggagcccc aagtgggttc atggcatcca 27660
gggagcagcc tcgggactgg gagggaaggc tcgtctgccg agctgtggtc gctctttctc 27720
cttggtgagt gtctggagcc caggcactct cctcactgtt gctctcattt ttcacagcaa 27780
taaaccactt ttacaacccc tcagctcact gggacctctg cctgtgtcac ccgccacccc 27840
cccgaatccc aacctggccg ccctcccccc acctcccccc cacagcgttt ccccccacca 27900
cccccaagcc tcctgggcac agcggccagc ctggggcctg gcggggccac gagcgactcg 27960
catgtgttgg ctgtgtcatc agcctgtgct gctgtcgcaa acaccgcagc cagtggggtc 28020
gccggacaaa ctgcaccaac tcagagctga gacttaggtc ttcctggggt gacatgaggc 28080
cgcagccggg gaggcagcac tgcagggagc tcgccggtac cgctcgctcc aagcaggagg 28140
ggactgaatg tcaccatgtg tgtgacagga gggagggcct gggaaggcca ggccaggggg 28200
tatcggggga cagaggtcgg gagaagggct ggcccaggga gcagagctgg acccggctca 28260
agcgctcagg acagacaggt cagggagaag gacgcggggg gtgcaaaggg gacctcgggc 28320
ccagacctcc gcctgggctg cccagagggc ctagcccgtc ccaggacaag ccgaggggcc 28380
gctcggggct ccggtgaggg accagggaag gggcctggtg gttgcgacca ggccgccgtg 28440
tccgccggcc ggctgacagc cgtccagggt gcggccagga ctccaacctc ccagaggtga 28500
tcccgcccca gaggaacagg cctcgggcca gggctggagg ggacaggact ctcccaaaag 28560
ggcagagcag ggggcccggc tgcccgtgct tcctagtcac agctccagga agggggacag 28620
ggccagcccg gggctgtccc agggaggcat ttgacgggag ccgggtcacc tggggacatg 28680
acctgggcca ctgaatgctg gcgctgaaag cctctcgagg cgaggggagg agccccaggc 28740
cgaggccagc accacggttt cccaggccag gagctgtggt cctgctcagc ctggcccaga 28800
gcccagggca tcggcaaggg cccctccagc gtcccgcagg gaacaggatg gagacggagc 28860
cagagcagca tgcagggaac ggactgcgtc gctccagggg tccctgaacc agacacgggg 28920
aggccgggac gtgcggccag agcatggacg tggggtggac agcagaggcc aggcatgcag 28980
ggcacggagc gccctccgcc tccaggttct gggggctcag gccacggtcc tggggctcac 29040
cccacaccct ccgtttccct caccctaaac gtctagccaa acctgaacga gcccatgggc 29100
cacgcagcct gctcagcccc aggccctggc cctcacgtct gtccccgcac cagccatcag 29160
cccctggccg gcccactccc gcgggaccgc cagcatggcg acagcggggc tggcccccgc 29220
accctcgaga actcccccac cggacccagg cctcccaccc tgacccccag caacgcctca 29280
tcccacggca accccacctc tccacgtgcc ccgcgcaccc cgagcccagg gcccgacgcc 29340
agcccctgag cccctcgggc tcccctgagt gagcctgaag ccagaccccc gcggcctcct 29400
gacccctaga cccccagcat tagctttaca cacaccggta ccatctcacc ctgcccacgc 29460
acacccacat ctgcgtcgta cacacacaca cacacacacc cccacgggca catggacgtc 29520
caggatttcc agctcagccc caaagccgag ggcactaagg ggcccctccg atcacagccc 29580
ccacgccccc ccaacttggg gccactcccg gaagagcgtg gctcccccgc agagagaacc 29640
ccgctggacg tgggtgccga acacccattc gcactgaacc accctcgaaa cagagcgcgg 29700
gcccacggtc cagcccacac tcagagccca cagccaaagc gagggctcag aggccaaggc 29760
cggcccacgg tccccgtgca ccctgctccg gagtccgaag ccagcccagc cccgccaggc 29820
ctccctcccc aggctcagcc cgcccctccc tgcccccagc tcccagtgcc cgtgcccccc 29880
gtgcccagcc cagtccctgt gcccacgccc acccctgcgg tccaccctcc ctgctggcca 29940
ccccaggccc caggcctgca gaatgaggag gcagagaagc cttctgggga gaggccgtgg 30000
gaccctgggg ctgcgggcaa ggccctcggg gtgggagggg gccctcacgg aaggagcttc 30060
ccggggggtc cacgcggcca ccgtctcggc ccagctgccc cacccccact caccgtggcc 30120
tcgccggggc ctcgctcctt tcttagcaaa gctccttctc cctgaggaaa ctgtgcaagt 30180
gggggaggct gccctgggta tcagggcggc tggggtgggg gcactgggga tctgtgtgtg 30240
tgtcgggggg tgcactgtgg ctctgcactg acggaggtgg gatgaacagg ggatttaccg 30300
gagtggggtg cactggggat ctgtgcaggt gcgatgggat tcccagggaa tcggttcagg 30360
agcaggtggc tttgccctgg ggatctgttt gcatggggtg gggtgcactg gtattctgtg 30420
cagaagacat ggcgtgcact agggattcgt gccggtgggg ttggggtgca ctggggatct 30480
gcgcaggtgg gggtggggtg agctggccgg ctctgcaggt ggggtggggt acactgggga 30540
tctgggcagt tgcggcgggg tgcagtgggg atctgcgcag gtgttggagt gcactgaggt 30600
ctgtagggtt ggggggcact gagtagctgt gcaagtgggg tgggagatgc accgggaccg 30660
gaagtgaggg ggtggggtgg gctgggatgg gagcaggtgg gtggggtgcc ctggggatca 30720
ggtagggggc cttcgtgatc actggcattg gcagggcagg gggtccgggc tctggcgagt 30780
gaatctgtac ctagacgaag gccaaagcac acgtaaatac atccacacct ctgactctca 30840
gctgcagccg cccgcccagg acacacgacc accatccgca ggaaccagcc caggcggcgg 30900
ctgcgccctc tgcctcagac ctgcctctgt ctacagggac agcccgccgg acaggaggtg 30960
ccctggccac cacacggggc cgagccgcgc tggccgaccc gctgcccctg ccccacggcc 31020
tccatcacca ccccgacacg ccctgtggga aggagccctc tgcacgcggg aatcctttga 31080
aaggtcggcg tacgcatcac acagggggtt cacctgtact gcctgtgcgc ctaatgggcc 31140
ctgctatgca cacgccacct tcacagtaag agtgaacact cccaggcgtc ggagacccac 31200
ggggcggccc cgccagcggg tgggggccgc tccctggggg ctcagaacac acggcctcgg 31260
ctttgccccg ggaaacacgt ccagtggagc aggagacaca ggtgtccggc aacggctcgg 31320
ggccgtggtt gcaaacggcg cccgatgcat cctgggacct ggacccaagg agggggcgag 31380
ggggaagctg gccaagccgc ctgggcaggg aggggatggc tcccggaggc ggaggtgagc 31440
agagaagggg gcggcggggc cggcccgggg cctgagcagc gccgcgggcg gggagcgtcc 31500
tgctgggacc accgctctgc cctgggcccg ccctcctcgt cctcacagga ctgacctcaa 31560
gccagggctg cccgcacctg aagggcacgc agaccacccc aggaggagac tccgggcctt 31620
tctctttttc gttttttaat ttcatttgac tttattctat attattttat attataccgt 31680
tttagggcca cacccacggc atttggaagg tcccagccta ggtgctgaac cagagcggca 31740
gcagccgcct gcaccacagc cacagcaaca ccagagccga gcagcctctg tgacctacac 31800
cgcagctcgc ggcgacgcgt cctcatggac gccagtcgga ttcattcgcg ccgcgccatg 31860
acgcaaactc ctctttcgca atttcttcca ttgaagggta gttttacacc gtcgtaaatt 31920
tgccatgttg tgttactttc aggtccccgg cacagcggcc cggccacaca cacacatatg 31980
catccacacc cacgtgcaga caggcagaca cacgtcagga cacatacaca gcggtgtgtg 32040
gagaggttga tcccaacctc ctggctcatc tccccacctc tcgccctggg gaagcgtgcg 32100
gtggccccat gtctgcgggt ccgtcgcggt tttgcttcga gtccactcgc acccctcctt 32160
ttaatcccac acgcaggcaa tgccgcacga tgtcggtctc cgtccgcgtc gcttcagcca 32220
gaggagcagg acgacctcta ggtccgacct cgtggtggca aatggcatcg ccgcatcctt 32280
tgcacggctg agcgataacc cgttgtgcct gtgcccctca ccttctgcaa ccgtccgtcc 32340
agacggacgt ttgggccgct tcctgtcttg gtcattgcga ggagtgctgc gacggatgct 32400
ggagagcccg tgcgttttca cgttgtggtt ctctcccact gtgtgcccag cactggggtt 32460
gccgcatcgg acggcagttc tgttcgcagt gctttcagga agctcaggcc gctttccgga 32520
gtggtcgtac ctacatcccc accgacagcg tagggggttc ccttttctcc acaccctctc 32580
cagtatatac atcccgtttg cagacttttt gatgatggcc actctggctg gattgaggcg 32640
ctacctcatg gcagttctga tttgcctttc tctaataatt agcaacagtg ggcacctttt 32700
catgcgtttt tggccaactg cacgtctgtc ttctttggag aaatgcctgt gtaggtcttt 32760
tgcctctttc ttgatgggat tcttcataat caagctgcat gccttgtttg tatgttttgg 32820
agatgaatcc cttggcggtc ccttcattgg caaataattt ctcccttttt gtaggttgtc 32880
tctttgtttt gcttgtggtt tcctttgcag tgccgcatat ttttaagttt aattagttcc 32940
cgtttgttta attttctttg tatttccatt gatctagaag acgtgtccaa agagatattg 33000
cgatggttca tgtcaaagtg tatcctgcct attgtcctcc aggacttaca catatctggt 33060
gctacattta catcttttaa tcattttgag gggtttgtgc gtgtgtgtgt gtacaccgtt 33120
ggagaatgtt ctacttttgc tattttacaa gtagctgtcc ggctttccca gcaccattga 33180
ttgaagaggc tgtctctact ctattgaata ttcttgtttc tttgtcatgg acgaattgac 33240
tgtaagtgct tgtgtttatt tctgggtttt ctatccagtt ccgctgatgt gtgtctctgt 33300
ttctgtgaca gtgccatact gttttcatga ctgcagcttt gtagtatagt cagaagtcag 33360
agatcctgat tccaccagtt ccatttctca tactcaacat gctttggcta tttgaggtat 33420
tttgtgtttc catacaaatt taaatttttt ctgttccagt tctgtgcaaa acgccactgg 33480
caatttgata gggatggcgt tggatgtgta gattgcatgg ttactgcggt catttggaca 33540
atgctgagtc ttccaatcca ggagcatggt ctggctttcc atctgtttgt gtcatctttg 33600
attgctttca tcagcgtctt ctagtcttca tagtacgggt cttttgcctc tttaggtagg 33660
tttgttcata ggtcttttat tcttttggat gcattgataa gtttgattat cccccacccc 33720
cccgcccttt ctggcctttt gttgcgagtg tgtagacatg cggtagattt ctgggcatta 33780
gtttgtatcc tgccacggta cctaatgcac tgatgagctc tagcagttcc ctggtggtat 33840
ctttaggact tcctacgtgc agcgccatgt cctctgcaaa cagtgagagt cttacgtttc 33900
tcttccaaaa ggacggcgtt agtttctttt tcttctctga ctgctggggc taggacttcc 33960
actaccatgt tgagcacacg gcggccaact atgtggtgag ggtggccatc ctagtcttgg 34020
tcctgatctt agtggaaatg agttcagctt ttcaccttgg agtgtattaa cggcgggctc 34080
ctcctatgga ctgtgttctg ttgaggggca ttctctgaga ccctgatgcc cccacgcaca 34140
agtccgagga gccctctgag gcagaagacc ctgggcttcc cgggggcccc cgagcctcga 34200
cgaccttctc ccctgtgggc atgctgccag cccgcgtgcc gattttatac acctttgatt 34260
tcctgggtgg catcacttct cacttgaaac ccctagaggg gcttatgttt cctggattcg 34320
ccctggactg gcacggcaac aagtgaaatg tcctagcaat acaatctgat ctcatgaaaa 34380
gagataaaac tcaaaataaa caatgtgaac ttacacctaa acatctagaa aaggaaaaag 34440
agacaacacc caatattagt agaggaaaga aatcacaaaa atcagagcag aaatcaatgg 34500
catagagatg aagaaaatga tacagaagat caattaaatc aaaatgtggt tccttgaaga 34560
cgtcaacaaa atcgccaaat ctttagccag cttcctcaag aaaaaaaggg agagagttca 34620
cgtctataaa attagaaatg aaaaagaagt tacaactgac accacagaaa tacaaaggac 34680
caatatcagt tcctccaatc caggagcatg ggctgtattt ccatttcttt gaatcctctt 34740
taatttcctt gataatgttt tatatttctc agcaaatagg tatttcacct ccttagtcag 34800
gtttattctg aggtatgtaa tttggggcct gctattttaa aagatgtcat agttttctat 34860
ttctttggta acactttatt gttcctatac agaaacgcaa ccaatttctg aatgttgctc 34920
ttgtatcctg ctactttgct aaattcattg atcagttcaa gcagtttatg agtggagacc 34980
ttagggattt ctctgtacag tgtcatgtca tctgcacaga atgacaattt cacctcttcg 35040
cttccatctt ggataccttt tatttcttct gtttgtccct ttgctgaggc taggacctcc 35100
aatactatgt tgaatagaag tggtgagact gggcatcgtt gccttgttcg taaaagacag 35160
caatggtggt aaggctttcg gcttttctct gttaggatta aagtggctgt gggtttgtga 35220
caaatggctt taatcttcga caaaggaggc aaggatataa aatggggaaa agacagtctt 35280
ttcagcaagt gttgctagct aaaacggaca gccgcatgta catcaatgaa actagaacac 35340
accctcacaa caggcacgca cacacacaca cacacacaca cacacacaca cacacacaca 35400
ctctctatat ggcttaaaga cttaaacaag acataacaaa actcttagaa gagaacacag 35460
gcaaaacatt ttctggcatc aaccacacaa atgatttctt aggtcactct cccaaggcaa 35520
cagaaacaaa accaaaaata aaccgatggg atgtaatcaa acgtacaagc ttttgcacag 35580
ccaaggaagc cgttaaagaa aagacaacct acggaataag agaaaagagt ttctaacaat 35640
gcaaccgaca agggcttagt ctctgaaata tacaaacagc ctacacaact caacagcaag 35700
aaacaccacc caactgaaag acgggcagaa gacctgaata gacatgtcgc caaagaagac 35760
aaagagatgg ccaacgagca cactaaaaag tgctcaacat cactgttcat tagagaaacg 35820
cgactcaaaa ctgctatgag gtaccaactc acacctctca gaatggccat catgaaaaag 35880
cccacaaata acaaatgatg gaaggggtgt ggaggcaagg gaaccctcct gcactgctgg 35940
tgggaatgta aactggtaca accactccgg aaagcagtat ggaggtacct caggaaacta 36000
aatgtagaac taccatatga cccagcaatc ccactcctgg ggatatatcc acacaaaact 36060
ttccctgaaa aagacacatg cacccatgtg tgttcactgg agcactagtc acgctcgcca 36120
agacatggaa acaacctaaa tgtccatcga caggggaatg gattaagaag atgtggtata 36180
tatacacact agaataccac tcggccgtaa aaacgaacca aataatgcca ttggcagcaa 36240
cacggatgga accagagact ctcatactaa gtgaagtaag tcagaaagac caataccata 36300
cgctatcaca tacgtctgga atctaatata tggcacaaat aatcctttcc acagaaaaga 36360
aacccatgga cttggagaaa atacttgtgg tcgccaaggg tgaggtagtg gggtagactg 36420
ggagtttggg gttagtagat gcaacatatt gcatttggca tagataaact atgagatcct 36480
ggtagagagc acagggagct gtatctagtc ccttgtgatg gaacatgttg gaggataaag 36540
tgagaaaaag aacgtataaa tatatacacc acacgcacac acacacacat atatgaatga 36600
ctgggtcact ttgccgtaga gtagaaattg acagaacaca gtaaatcaac tataacggaa 36660
aaaaataaaa accatttaaa aaaaaaaaaa aacagatgct cgtctccatg gattctgaac 36720
acatttttga cagaattcaa cacccaactt tttcttgatg gtttttattt tttccactgc 36780
aggtggttta ccctgctctg ttgattacta ctgtacagaa aagtgacaaa gtcacacata 36840
tatatatata tatatagaca ttctttttct cacattatcc tccatcatgc cccatcatag 36900
gtgactagat agagggaaac tacaccaaca tactaaaagc catatatgac aaacccacaa 36960
ctatcatcat tctcaatggt gaaaacctga aagcatttct gctaagatca ggaacaagac 37020
aaggatgtct gctctcacca ctctccttcc acggagtttt ggaagtccta gccagggcaa 37080
ttagagaaga aaaggaatcc aaatcggaaa cggagaagta acactaccgc tctttacagg 37140
tgacgtgctg cgatacctag agaatcctca aggcactacc agaaacctct tagagctcgc 37200
caatgaattt ggtcaagctg caggatacaa aattaacaca cagaaattgg ctgccttgct 37260
ctacgttaac aatgaaagag cagcgagaga aactagggaa accatcccac tgagcagtgc 37320
ctcgaaatga atgaaatgcc caggaataaa cctacccaaa gacacaaaga cctggactct 37380
gaaaaccata aggcactgct ggaagcaacc aaagacgaca caaaccggtg gaaagctaga 37440
ccatgctcct gccctggaag aattaatact ggcaaaaggg ccataccacc taaggccacc 37500
ctcactctca gtgcaatccc tctcaaatct ccaatggcat tttccacaga actaaaagaa 37560
aaagggtttt aatttgcatg gaaacacaaa tacctcactg acctgggcta tactacaaag 37620
ctgcagtcat caaaacggta tggcactgac actccccccc aaaaaagaca tacatatcag 37680
tggaacagaa cagaaagccc agcaataaac ccaagcacct atgctcaatc catccatgac 37740
ataggaggca agactattca acagagaagg gagagtcact tcactaagtg gtgctgggga 37800
gctggacagc tccacgtaaa gaatgaaacc agaactctcc cgaacgccaa acacaccaaa 37860
aaaaataaat aaataaaaaa ataaaaaaac cctcaaaaca aatgaaactc ctaaacgtaa 37920
gactggatac ctataactca cagaggaaaa caggcagaac gttctttgac ataaatcaca 37980
gcaacatctt atttgatcca cctcctggaa taactacaat aaagacaaaa gtggagttcc 38040
cgtcacggct cagtggtaac caaatctgac taggaaccat gaggttgcgg gttcaatccc 38100
tggccttgat cagtgggtta aggatccggc gttgccatca gctgtggtgt aggttgcaga 38160
cgcggctcgg atcccacgtt gctgtggctc tggtgtaggc cgacagctac agctccaatt 38220
cgacccctag cctgggaacc tccacatgcc atgggtgcgg ccctggaaaa gacaaaaaga 38280
cggggaaaaa atgaaagaga gaaagaagga aagaaggaaa ggaggaaaga aggaaaggag 38340
gaaagaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag 38400
aaagaaagaa agaaagacag acaaaagtaa accaatgaga tctaattaaa ctcaaaagct 38460
tttgaaaagc aaaagaaacc attttaaaag agaaaaacag gagttcccgt cctggctcag 38520
gggttaacga atctgactag catccatgag gaggcagatt tgatccctgg cctcactcag 38580
caggttaagg atccggcatt gccatgagct gtgagctgtg gtgtaggcca gcagccgtag 38640
ctccaattca acccctagcc cgggaacctc catatgccac aggtggggcc ctaaaaacca 38700
aaaaagacaa aggaaaacaa cccacagaat gggacaaaat ctttgcaaac aatgcagccc 38760
accaaggctc aatccccaaa atatacaaac actctacaac tcaacaacaa caaaaaacaa 38820
accaacaaac aacccaatcg ataaatgggc agaagaccta aatagacatt tctccaaaga 38880
agacatacag atggccagaa gacacatgaa aaaatcctca acagcactaa tttttaggga 38940
aatgcaaatc aaaactgcaa tgaggcacca cctcacactg gtcagaatgg ccatcattaa 39000
gagtcagcta acagcaaatg ctggagaggg tgaggagaag agggaccccg ccttcactgt 39060
tggtgggaat gcaacttggt acaagcactg tggaaaacag tatggaggaa cctcagaaaa 39120
ctgaacgtaa aaccacgatt gaacccagaa atctcacccc tcggcctgta tctcgatacg 39180
actttcagtc aaaaacttac atgcacaact atggactttg cagaagggtt caaaatgggc 39240
aggacatgaa aacactcaaa cgcccatcaa cagacgaacg gattaagatg tggttctcac 39300
acaaagtaag aactacgtct cagccgtaaa aaaggaccaa gtcacgccat ttgcagcaac 39360
gtggatggaa ctagagactc tcacactgag tgaagtaagt cagaaagaga aagacagaga 39420
ccacatgaca ccacttatat gtggaatcta aaatacggcg cgattgatac tgcggacaaa 39480
acagagacag agcatggtca tggagagcag cctttgcttt ccagggggag ggagagaaga 39540
gattgacagg gggtttggag ctggtagatg caactggtca cattgaaaac gagtaagtga 39600
tgggtcctac tgggcagcac acggaacagt gcccggcctc ttgcgttaga acgtgagaga 39660
ggaaggagag tgtgaaaaga aaaagaaggt aaatgtatgt acagctgtac aagggtaatt 39720
gaaggaacgt tgtaaatcaa ctctggtttt aaaaaaaaaa aaaaaattta aagaaagaaa 39780
acgttctcca gaggcagaac gagaaggtgg aacggccgac aggtctctgt cctgcagcgg 39840
ccgctgacac gaccccagga cagcggaaat gagacccagg ctgcctgtcg ggcacacggc 39900
ggccgccccg gcaccctggt gatcctgtgc agcacgaggt gcccagtaga ggggtgggca 39960
gggcagagca gggcagggca ggacctgggg acgcggccgc agcaggtggg ctccagccag 40020
gaaggagcca cgagtggggt ggggtctgct cgctgggctg gaggcaggga gggcacctcg 40080
gctccaacat cccagccggg cacccggcca gaggaagggg ctgcgaggat gtcttccaag 40140
catctctttg ctcttggaac cacgtggcga agctttctga aagcagacca gactgcagca 40200
ccacggtttc tattgtgaag ggatttttcc agaaggagtg ggaccttgcg aacggggatg 40260
tggacacagg gtggcttctg atcaagccca gggccctgtg cagccaagtc tcatggtcac 40320
gccgtcccgg ccggaccagc ctgcccccca cgcggacccg gggaaggcag gggtcccatg 40380
accagcacga gaacgactga ctcgccttga cctcaagcca cctccaggac caggggcaga 40440
cggcccggca ccacgaggag gaggcagagc cccgggcagg gcccacttgc cacttcacct 40500
cgccagagag ccgggcagat ggcggcacgc gactcttggg gcctgacggc cccgagccca 40560
gacgctctcg gggctttggc cagactcgca gggctgctga gccaagcaga ggctacaaag 40620
ctccacgggc ttccagggca accgctagag acacgtgggc acagctcgca gagccggtga 40680
cccacgggcc agatgggaac ctccctcctg tgccccctca aggcgtctcc cggaacggtg 40740
gcctcccacg gcgctggacg gggctcgggg caggcccgag gcagcacggc gcccacaccg 40800
tccactggcc gcttggaagg attcagccaa caccacccca ggcccgctgc acagccgggg 40860
tgggggctga gacgtccctc aggggtcccc agtcccagcc tgatgctgaa gttacaggac 40920
ggcccagggg cgcccagggg acagggggag ctgcggccct ccacgaatga ggacagaggg 40980
tccctgaccc ccacgcacgg ccggcaggtc tcggtccacg cagagcagtg agcggctgcc 41040
cgccagcccg ctctcggccg ggcaccacct tcacaacccc tgggaactct gtccccacgt 41100
gggcagcacg ggcctgggtc tgggcagcag gctgggccca gccacacacg cgggggtcac 41160
aagaggtgcc cggtggggtc gggcaaccaa gggctcacac taggggcctg gcacccgggc 41220
tgcagtgggc caggcggcat aatccccgtg ggacatagag aaccacgacg tcctgagttc 41280
ccgcaccctc cccaccgctc acccacccca cacacccaca tgagccgcca gggcactgtc 41340
tggactcccc aggccctctg ggacagaaag caaccagaag aaaagggaac ttcaggaagc 41400
agccgggcca cccggtttca atcccattct tagtgtgcag ggctctgggc aggaaggtcg 41460
tgcctccagg gaccagacac ccacctgagg aagcagggct gcaggcacag agcacagaca 41520
caaggagcca cttgcacctg gggccagagc aggaggggcc cggagcccgg ggcagcaggg 41580
gagccacctc ctcggacgct acactcggtg cgtggggtcc acgaggactg gggcaggggc 41640
agcggcgggg ggacgaggag gggggacccc acagcagggc tgcagccgac ccagcccagg 41700
gatcgcagcc tgcacgcggg tcaaagcacc tgcccagcac agggaccagg cagccctggc 41760
aggggcacac ccatgccaac agagctggcg ccccagaggc caggggaggt cagtggggcc 41820
gccaggcctc tggggaacat gatgggctgg ggtcggaggc ctgagggatc tgctcggcca 41880
ggttgctgga ggcacagggc tgggcagggg ctgaggacga ggccaggatg gacaggtggg 41940
tctgggcgga ggacccaggg aagagacgga cacagcgcag gggacaaaga ggtcacctgg 42000
ggttgcaggg tcctgtcccg gctgccggag cccgcttgag ccccagggca ggtatgaaag 42060
cagtaactgt cgggtcccgg ggaaggagcc agggccacgg gcagcaggtg agccgggagc 42120
agagcaggca gggcgggccg ggctcctgtg tgcccagagc tggcagcaca gggactgacc 42180
ctaaagctcc acaggccgca ggagggcagg agcgggagcc aggggctggg agctgggcgc 42240
cagtgcagct gagccagcaa ctagagggag ccagggacca gacaggctcc aagggtggtc 42300
gcggcgagaa gagggtgagc tcagaagggt caggagcccc tccgggaggg gaccaggtgg 42360
gacagaggct ctgccagggg gacgctaccc agagagggtg ggcacccagg gctccgggaa 42420
caaaggccag ccctgggaaa gaaaggaagc caggagtgga gctagacaag gctgagggtt 42480
cgggggcagg aagcggggtg gagatgctga ggcccaggag ccccagggaa aggggcaggt 42540
aggctgcaag tgagcaggga accgagaagg cagaagaact gtccgtagag ctgctgggaa 42600
cacacagagc tggtgcaggt gagcaggggg ctggcaggcc agaggggtat tacctggagc 42660
tcctgggagc aggtagagct tgtgcaggtg agcagggctg ggggggcagg ggaagccctg 42720
caacctcctg ggaacaggta caagcttgta caggtgagca gcagctgata ggtcaggggg 42780
caggcctggc agctcctggg agaagttaga gcttgtgcaa ttgagcatgg gctgggacga 42840
gggggctgca cctgtaggtt ctgggaccag gtagagctaa ggcaggtgag cagaacctgg 42900
agggcaaggg gctgaacctc gagctcctgg aggcggttga gcttgaggag ctgagcatgg 42960
gctcagaggg caggggcttc ccctggaggt cttgggagca ggagcagctt gtgcggttga 43020
gcagggggtg ggagtgcagg gagcagtcct gggaactcct gggaccaggt agagcttgtg 43080
caggtgagca ggggttggta ggacaggagc tgaagatgaa actctgggga gcaggtgtag 43140
cttttacagg tgaagaggcc ctgggagggc tgggggcagt cctcggatcc tgagagctgt 43200
agatcttcgg caggtgggca caagctcgga gggcagtgga agaacctgga gctcctgggt 43260
caggagcagt gtgtgcaggt gatcaggggc tggagggcag ggggctaaac ctggagctat 43320
tgggaacagt ttaggcttgt gctggcaaac aggggatggg agggcaagga tcagtcctgg 43380
gagcccctgc gagccggtag agccccggca ggtgagcagg ggctcggagg gcagggagca 43440
gccctggccc ctcctgggag aaggaggagc tgtgcaggtg agaagggcta gggggaatgc 43500
aggccagggc aggggcagca gcagaggggc tgagctcagg ggagccccag gctggaggga 43560
ggggccggta cagggctagc caggctggaa agtggacccc tgggcggaag gtcgccagga 43620
ctccaggggc aggggcaggc ctgaacagac accatgggcc acagggctcg ggccaaagga 43680
cccgaagcct cgtcgaacaa agtgagggaa catggggcag ggtagctgga gtccgaactg 43740
agaacagcgg cacagggcag tgcacatggt cggggggtcg aggggccctt ctgggactgg 43800
gctggagctg caggcaatgg ctgaacagaa caggggggag aggaacgccc cagccaggca 43860
gggggcctgg ggtgctggcg ctccgcgaga gaggaaggcc tggggcaggg ctcggctgca 43920
gcagtgctgc caggcaggct ggcagcggga agggcaggag cagagggagg gctctggcct 43980
cagcaggcag ctgggaggcc cagagctggg ctgcaggggc cggggctctg gggaaagctg 44040
cctcgggtga gctcagggcc aagccaggag ccccggtgga ggagggaccg gtgggcccag 44100
aggcccagat ttggagcctg ggccagggcc ggagggatgg caggggaggg gcctcggagg 44160
agactacctc ccctcgccca gggaaagggg catgttggtg ccagggacca acgcgcacgg 44220
gccttgactg cagagctgac ccagaggaac ggggcctgga gagcaaacag caggccagga 44280
ccaggggcca ggagcagagc atgggcagtg ggtggcagtg ggtggcagtg tccctgccag 44340
gctggggaag caggacggtg gcctcagggg accagcagaa gctggccagg aacacaggcc 44400
acccgctggt cgggccaggc acccacaggg gctctgggcc ctggcgggtt ctcatccagg 44460
cggtcaccca gctcttacca ggcagctgcg cccagaacct gcagctgcaa tggcagcgag 44520
ctgggcggct ggtctgccga ctttctggaa gcaagtgggt gctgggcgca gcggccccgc 44580
tgttctgagg gcccgattcg ctgcccgccc cacaaggaac aaggccctgg cggtcacgca 44640
gcctcctctc ttccagcccc caagacggcc ccatcggtct accctctggc cccctgcagc 44700
agggacacgt ctggccctaa cgtggccttg ggctgcctgg tctcaagcta cttccccgag 44760
ctagtgaccg tgacctggaa ctcgggcgcc ctgaccagtg gcgtgcacac cttcccatcc 44820
gtcctgcagc cgtcagggct ctactccctc agcagcatgg tgaccgtgcc ggcccacagc 44880
ttgtccagca agcgctatac gtgcaatgtc aaccacccag ccaccaaaac caaggtggac 44940
ctgtgtgttg gtgagtgcct gggctggggg cgggtgtgca gacgggctga ggtcagcctt 45000
cctgccgaga gaccacccca ccagggtgtc agggccgctc acctctgtcc ccttcacctg 45060
caggcccagg ctcagggagg ggtcaactcg gcacttcgga gaggccaggg tgggcacagg 45120
cgggacccct cccccggccc tgggccccca gcagcacgag ctgctcaggg atgtaccaag 45180
acctgaccct gacctgagcc cagccctgga ccctccccac ccccagtgac tctgtgtgtt 45240
ctctcctcag gacgaccatg tcccatatgc ccaggtaaga aactcgggcc tcgccctcct 45300
ccccaaggag gtagcctgag cccaggttcg gcccgagggg ggacaggtgc ccaggaggac 45360
cccaggccac gagctgaccc gcactctgtc tcccccacca ggctgtgaag tggccgggcc 45420
ctcggtcttc atcttccctc caaaacccaa ggacatcctc atgatctccc ggacccccga 45480
ggtcacgtgc gtggtggtgg acgtcagcaa ggagcacgcc gaggtccagt tctcctggta 45540
cgtggacggc gtagaggtgc acacggccga gacgagacca aaggaggagc agttcaacag 45600
cacctaccgt gtggtcagcg tcctgcccat ccagcacgag gactggctga aggggaagga 45660
gttcgagtgc aaggtcaaca acgaagacct cccaggcccc atcacgagga ccatctccaa 45720
ggccaaaggt gggcaaggtg ggcaaggtgg gcaggcggag agggtcccgt ggggccgcct 45780
ggggggacca tcatgctaac tgaggtgcct ggcttcacag gggtggtacg gagcccggag 45840
gtgtacaccc tgcccccacc cgccgaggag ctgtccaaga gcaaagtcac gctaacctgc 45900
ctggtcaaaa gcttcttccc gcctttcatc catgttgggt ggaaaatcaa cggacagctg 45960
gagccagagg gcaattacca caccaccccg ccccaggagg acgaggacgg gacctacttc 46020
ctgtacagca aactcgcggt ggacaaggcg aggttgcaga gtggaggcat ccactgtgcg 46080
gtgatgcacg aggctctgca caaccactac accgagaagt ccgtctccct gactccgggt 46140
aaatgagcca ctcgctgcac cccacgtgct ctcgggtccc acgagctcac ctgagcccca 46200
gccctgtgta catatctccc gggccagcat gaaataaagc acccaacacc accctggcac 46260
ccgcaatgct gtcatggttc tttccgaggc agagccccag tgcaggcgcc acccgcgggg 46320
ccagggacag gacaggctct gggccagagg cgtccccgtg gcgtcggggg gagcagtccc 46380
acctgggcca gacccggtca gtgggggtct gcacaggcac cctgggggct gcccgaggcc 46440
gaggagggag ctttgcctga ggcggcctct cctgcgggct gccctcctgg cacacctgct 46500
ccggctgctg tgtgtggacg gacgggagaa gccctccagg ccccctcggg gaaatgactg 46560
cccaggtcgt cccgcctctg tccaagccca agctcagggg cagcggcccg tccttgtccc 46620
caggttgcct gtctcctggg tctcctgtgc ccacagggtg gggacggccc tgctcctccc 46680
gcaaacaccc gacatcaggc ccccaccccg cctcaggcca cggggctctg ccctgctggt 46740
ccccgcaggg ggccaggcct gggcactcgt ggcaacactc acccctgtgt cccacagggg 46800
cagaggtagg cagcccagag tgtccagggg agggacagtc ccagacaccc ccaggagtgg 46860
agcccagacc cactgatatc aagcccctcc caccgagctg cccagcaccc cccaccccac 46920
cgccttcctc cactcgcaca cccgggctct cgcacacgca tgcacgccgc ccccacctcc 46980
cagcacaccc gcccaccgcc tgcatccaca tgcacacaca cccgggcaca cacacaccta 47040
cccacgcaca catgtgtaca cccccgggcc ctcacagacc agagcatgga gctgggtctc 47100
acacaccctc accggggaca ccagcccggc cccatccccc gaggccccac gggctgctca 47160
gccacactgg cacactagac cacacggacc cgctccgacg cccggccccg ctcttcccct 47220
ggcacctacg ccccctccgg aacaagcaga aatccccagc ccgcttgtcc ccagcacagc 47280
cgcccccgcg gcccggggac acacacccca aggccgtctc ggcccttccc tgggccagag 47340
cccaccgggc ccctgctcgg cttaacgccc accctccggg cctggccccc tcggagagag 47400
ggcggggcct gagccactgg cctgcacggt caggacaccc cctcccagac ggtccccatg 47460
ctcgggggcc cccaccagag gagagggggc aggacatccc tggccaggct gagactcagc 47520
ccaggatgca ggactggggg gccggggccg gggccaggca ctgagctcag ccggggggtc 47580
ccccacctgg agccacccca ggccccgcgg gctggcacag cactgaccac ccctccccgt 47640
gcagaactgc tcctggagga gagctgtgcc gacgcccagg acggggagct ggacgggctc 47700
tggaccacca tctccatctt catcacgctc ttcctgctca gcgtgtgcta cagcgccacc 47760
gtgacgctct tcaaggtgag cgggccagcc ccgctgtcac ccgtgccccg gagccccctg 47820
ccggcccctc cctggccctc cctgtggcca cgctgtcact gtgacggccc ctccctgtac 47880
ccgccctggc ctctggtcct ctccgggaca cggtggctgt cggggaccgc ggggctggca 47940
ggcggaccct ggggggcggt ccacgtgcgg cccgggccgc cctcaccccg tgcgccctgc 48000
aggtgaagtg gatcttctcg tcggtggtgg agctgaagcg gaccattgtc cccgactaca 48060
gaaacatgat cggacaggag gcctagggcg ccctccaggg cggccagggc cgccccagac 48120
ccgacaggac cgcgtccctc agctcaagct gctccgcctg caccaggcct gcctccgccc 48180
tctgacctcg cccacctctg gctctcggcc acctcgcccg cacctctgcc cttgggcatc 48240
gcggccccct cagaaggcgc cccgcacccc caccgcccct cggaagcccg agtgggcagc 48300
agctcgggcc ccgacctgac ttgatggaca cccaccacca ccagcccccg ccagagccac 48360
gggacaccgc cagggccacg tgtcctcacc aggaacgccc ggctcagcca gcaggtccag 48420
ccggccacga ggagaccggc gtccagcggc tgacgggaca gggcccgggc tccctgtgga 48480
ccccgtccag accgggctcc agaggcaggg tccccgagag gaggctcctg tgtcacagcc 48540
tggagtggga acagctgccc ccaacgccag catctcccag cagggaagtt cccagacggg 48600
ccaccaacag gcgctcaggt cacctccagc ccagggcact cgagggagga ggacctgggc 48660
ccaaggccac ggcaggagcc ccggtgggga cagagcggcc ctgcccaggg aggcggtgag 48720
gtgccgccag accggcctcc tggagggggt cccgctgact cggacccccg caggcaggat 48780
gggccatcgc tggacacagg ctggggtgga agggccaccc gggattcaga gcagccagcc 48840
catcccgagg tccacggcct gagtcacgtc tatcggacca tccgtccatc cagcaggaga 48900
tccttctgcc tgccccgggg cctgggtgag aggtgaagaa cccccaggag cgagccccag 48960
gccccggacc cccaaggccg ccccagaaca gactctcccg ccctgtctct gcgggagccc 49020
caagtgggtt catggcatcc agggagcagc ctcgggactg ggagggaagg ctcgtctgcc 49080
gagctgtggt cgctctttct ccttggtgag tgtctggagc ccaggcactc tcctcactgt 49140
tgctctcatt tttcacagca ataaaccact tttacaaccc ctcagctcac tgggacctct 49200
gcctgtgtca cccgccaccc ccccgaatcc caacctggcc gccctccccc cacctccccc 49260
ccacagcgtt tccccccacc acccccaagc ctcctgggca cagcggccag cctggggcct 49320
ggcggggcca cgagcgactc gcatgtgttg gctgtgtcat cagcctgtgc tgctgtcgca 49380
aacaccgcag ccagtggggt cgccggacaa actgcaccaa ctcagagctg agacttaggt 49440
cttcctgggg tgacatgagg ccgcagccgg ggaggcagca ctgcagggag ctcgccggta 49500
ccgctcgctc caagcaggag gggactgaat gtcaccatgt gtgtgacagg agggagggcc 49560
tgggaaggcc aggccagggg gtatcggggg acagaggtcg ggagaagggc tggcccaggg 49620
agcagagctg gacccggctc aagcgctcag gacagacggg tcagggagaa ggacgcgggg 49680
ggcggggaag gggaccgtgg gcgcagacct ccgcctgggc tgcccagagg gcctacccat 49740
cccaggacaa gccgaggggc cgcacggggc tccggtgagg gaccagggaa ggggcctggt 49800
ggttgcgacc aggccgccgt gtccgccggc cggctgacag ccgtccaggg tgcggccagg 49860
actccaacct cccagaggtg atcccgcccc agaggaacag gcctcgggcc ggggctggag 49920
gggacaggac tctcccaaaa gggcagagca gggggcccgg ctgcccgtgc ttcctagtca 49980
cagctccagg aagggggaca gggccagccc ggggctgtcc cagggaggca tttgacggga 50040
gccgggtcac ctggggacat gacctgggcc actgaatgct ggcgctgaaa gcctctcgag 50100
gcgaggggag gagccccagg ccgaggccag caccacggtt tcccaggcca ggagctgtgg 50160
tcctgctcag cctggcccag agcccagggc atcggcaagg gcccctccag cgtcccgcag 50220
ggaacaggat ggagacggag ccagagcagc atgcagggaa cggactgcgt cgctccaggg 50280
gtccctgaac cagacacggg gaggccggga cgtgcggcca gagcatggac gtggggtgga 50340
cagcagaggc caggcatgca gggcacggag cgccctccgc ctccaggttc tgggggctca 50400
ggccacggtc ctggggctca ccccacaccc tccgtttccc tcaccctaaa cgtctagcca 50460
aacctgaacg tgcccatggg ccacgcagcc tgctcagccc caggccctgg ccctcacgtc 50520
tgtccccgca ccagccatca gcccctggcc ggcccactcc cgcgggaccg ccagcatggc 50580
gacagcgggg ctggcccccg caccctcgag aactccccca ccggacccag gcctcccacc 50640
ctgaccccca gcaacgcctc atcccacggc aaccccacct ctccacgtgc cccgcgcacc 50700
ccgagcccag ggcccgacgc cagcccctga gcccctcggg ctcccctgag tgagcctgaa 50760
gccagacccc cgcggcctcc tgacccctag acccccagca ttagctttac acacaccggt 50820
accatctcac cctgcccacg cacacccaca tctgcgtcgt acacacacac acacacacac 50880
ccccacgggc acatggacgt ccaggatttc cagctcagcc ccaaagccga gggcactaag 50940
gggcccctcc gatcacagcc cccacgcccc cccaacttgg ggccactccc ggaagagcgt 51000
ggctcccccg cagagagaac cccgctggac gtgggtgccg accacccatt cgcactgaac 51060
caccctcgaa acagagcgcg ggcccacggt ccagcccaca ctcagagccc accgccaaac 51120
cgagggctca gaggccaagg ccggcccacg gtccccgtgc accctgctcc ggagtccgaa 51180
gccagcccag ccccgccagg cctccctccc caggctcagc ccgcccctcc ctgcccccag 51240
ctcccagtgc ccgtgccccc cgtgcccagc ccagtccctg tgcccacccc cacccctgcg 51300
gtccaccctc cctgctggcc accccaggcc ccaggcctgc agaatgagga ggcagagaag 51360
ccttctgggg agaggccgtg ggaccctggg gctgcgggca aggccctcgg ggtgggaggg 51420
ggccctcacg gaaggagctt cccggggggt ccacgcggcc accgtctcgg cccagctgcc 51480
ccacccccac tcaccgtggc ctcgccgggg cctcgctcct ttcttagcaa agctccttct 51540
ccctgaggaa actgtgcaag tgggggaggc tgccctgggt atcagggcgg ctggggtggg 51600
ggcactgggg atctgtgtgt gtgtcggggg gtgcactgtg gctctgcact gacggaggtg 51660
ggatgaacag gggatttacc ggagtggggt gcactgggga tctgtgcagg tgcgatggga 51720
ttcccaggga atcggttcag gagcaggtgg ctttgccctg gggatctgtt tgcatggggt 51780
ggggtgcact ggtattctgt gcaaaagaca tggcgtgcac tagggattcg tgccggtggg 51840
gttggggtgc actggggatc tgcgcaggtg ggggtggggt gagctggccg gctctgcagg 51900
tggggtgggg tacactgggg atctgggcag ttgcggcggg gtgcagtggg gatctgcgca 51960
ggtgttggag tgcactgagg tctgtagggt tggggggcac tgagtagctg tgcaagtggg 52020
gtgggagatg caccgggacc ggaagtgagg gggtggggtg ggctgggatg ggagcaggtg 52080
ggtggggtgc cctggggatc aggtaggggg ccttcgtgat cactggcatt ggcagggcag 52140
ggggtccggg ctctggcgag tgaatctgta cctagacgaa ggccaaagca cacgtaaata 52200
catccacacc tctgactctc agctgcagcc gcccgcccag gacacacgac caccatccgc 52260
aggaaccagc ccaggcggcg gctgcgccct ctgcctcaga cctgcctctg tctacaggga 52320
cagcccgccg gacaggaggt gccctggcca ccacacgggg ccgagccgcg ctggccgacc 52380
cgctgcccct gccccacggc ctccatcacc accccgacac gccctgtggg aaggagccct 52440
ctgcacgcgg gaatcctttg aaaggtcggc gtacgcatca cacagggggt tcacctgtac 52500
tgcctgtgcg cctaatgggc cctgctatgc acacgccacc ttcacagtaa gagtgaacac 52560
tcccaggcgt cggagaccca cggggcggcc ccgccagcgg gtgggggccg ctccctgggg 52620
gctcagaaca cacggcctcg gctttgcccc gggaaacacg tccagtggag caggagacac 52680
aggtgtccgg caacggctcg gggccgtggt tgcaaacggc gcccgatgca tcctgggacc 52740
tggacccaag gagggggcga gggggaagct ggccaagccg cctgggcagg gaggggatgg 52800
ctcccggagg cggaggtgag cagagaaggg ggcggcgggg ccggcccggg gcctgagcag 52860
cgccgcgggc ggggagcgtc ctgctgggac caccgctctg ccctgggccc gccctcctcg 52920
tcctcacagg actgacctca agccagggct gcccgcacct gaagggcacg cagaccaccc 52980
caggaggaga ctccgggcct ttctcttttt cgttttttaa tttcatttga ctttattcta 53040
tattatttta tattataccg ttttagggcc acacccacgg catttggaag gtcccagcct 53100
aggtgctgaa ccagagcggc agcagccgcc tgcaccacag ccacagcaac accagagccg 53160
agcagcctct gtgacctaca ccgcagctcg cggcgacgcg tcctcatgga cgccagtcgg 53220
attcattcgc gccgcgccat gacgcaaact cctctttcgc aatttcttcc attgaagggt 53280
agttttacac cgtcgtaaat ttgccatgtt gtgttacttt caggtccccg gcacagcggc 53340
ccggccacac acacacatat gcatccacac ccacgtgcag acaggcagac acacgtcagg 53400
acacatacac agcggtgtgt ggagaggttg atcccaacct cctggctcat ctccccacct 53460
ctcgccctgg ggaagcgtgc ggtggcccca tgtctgcggg tccgtcgcgg ttttgcttcg 53520
agtccactcg cacccctcct tttaatccca cacgcaggca atgccgcacg atgtcggtct 53580
ccgtccgcgt cgcttcagcc agaggagcag gacgacctct aggtccgacc tcgtggtggc 53640
aaatggcatc gccgcatcct ttgcacggct gagcgataac ccgttgtgcc tgtgcccctc 53700
accttctgca accgtccgtc cagacggacg tttgggccgc ttcctgtctt ggtcattgcg 53760
aggagtgctg cgacggatgc tggagagccc gtgcgttttc acgttgtggt tctctcccac 53820
tgtgtgccca gcactggggt tgccgcatcg gacggcagtt ctgttcgcag tgctttcagg 53880
aagctcaggc cgctttccgg agtggtcgta cctacatccc caccgacagc gtagggggtt 53940
cccttttctc cacaccctct ccagtatata catcccgttt gcagactttt tgatgatggc 54000
cactctggct ggattgaggc gctacctcat ggcagttctg atttgccttt ctctaataat 54060
tagcaacagt gggcaccttt tcatgcgttt ttggccaact gcacgtctgt cttctttgga 54120
gaaatgcctg tgtaggtctt ttgcctcttt cttgatggga ttcttcataa tcaagctgca 54180
tgccttgttt gtatgttttg gagatgaatc ccttggcggt cccttcattg gcaaataatt 54240
tctccctttt tgtaggttgt ctctttgttt tgcttgtggt ttcctttgca gtgccgcata 54300
tttttaagtt taattagttc ccgtttgttt aattttcttt gtatttccat tgatctagaa 54360
gacgtgtcca aagagatatt gcgatggttc atgtcaaagt gtatcctgcc tattgtcctc 54420
caggacttac acatatctgg tgctacattt acatctttta atcattttga ggggtttgtg 54480
cgtgtgtgtg tgtacaccgt tggagaatgt tctacttttg ctattttaca agtagctgtc 54540
cggctttccc agcaccattg attgaagagg ctgtctctac tctattgaat attcttgttt 54600
ctttgtcatg gacgaattga ctgtaagtgc ttgtgtttat ttctgggttt tctatccagt 54660
tccgctgatg tgtgtctctg tttctgtgac agtgccatac tgttttcatg actgcagctt 54720
tgtagtatag tcagaagtca gagatcctga ttccaccagt tccatttctc atactcaaca 54780
tgctttggct atttgaggta ttttgtgttt ccatacaaat ttaaattttt tctgttccag 54840
ttctgtgcaa aacgccactg gcaatttgat agggatggca ttggatgtgt agattgcatg 54900
gttactgcgg tcatttggaa aatgctgagt cttccaatcc aggagcatgg tctggctttc 54960
catctgtttg tgtcatcttt gattgctttc atcagcgtct tctagtcttc atagtacggg 55020
tcttttgcct ctttaggtag gtttgttcat aggtctttta ttcttttgga tgcattgata 55080
agtttgatta tcccccaccc ccccgccctt tctggccttt tgttgcgagt gtgtagacat 55140
gcggtagatt tctgggcatt agtttgtatc ctgccacggt acctaatgca ctgatgagct 55200
ctagcagttc cctggtggta tctttaggac ttcctacgtg cagcgccatg tcctctgcaa 55260
acagtgagag tcttacgttt ctcttccaaa aggacggcgt tagtttcttt ttcttctctg 55320
actgctgggg ctaggacttc cactaccatg ttgagcacac ggcggccaac tatgtggtga 55380
gggtggccat cctagtcttg gtcctgatct tagtggaaat gagttcagct tttcaccttg 55440
gagtgtatta acggcgggct cctcctatgg actgtgttct gttgaggggc attctctgag 55500
accctgatgc ccccacgcac aagtccgagg agccctctga ggcagaagac cctgggcttc 55560
ccgggggccc ccgagcctca acgaccttct cccctgtggg cattctgcca gcccgcgtgc 55620
cgattttata cacctttgat ttcctgggtg gcatcacttc tcacttgaaa cccctagagg 55680
ggcttatgtt tcctggattc gccctggact ggcacggcaa caagtgaaat gtcctagcaa 55740
tacaatctga tctcatgaaa agagataaaa ctcaaaataa acaatgtgaa cttacaccta 55800
aacatctgga aaaggaaaaa gagacaacac ccaatattag tagaggaaag aaatcacaaa 55860
aatcagagca gaaatcaatg gcatagagat gaagaaaatg atacagaaga tcaattaaat 55920
caaaatgtgg ttccttgaag acgtcaacaa aatcgacaaa tctttagcca gcttcctcaa 55980
gaaaaaaagg gagagagttc acgtctataa aattagaaat gaaaaagaag ttacaactga 56040
caccacagaa atacaaagga ccaatatcag ttcctccaat ccaggagcat gggctatatt 56100
tccatttctt tgaatcctct ttaatttcct tgataatgtt ttatatttct cagcaaataa 56160
gtatttcacc tccttagtca ggtttattct gaggtatgta atttggggcc tgctatttta 56220
aaagatgtca tagttttcta tttctttggt aacactttat tgttcctata cagaaacgca 56280
accaatttct gaatgttgct cttgtatcct gctactttgc taaattcatt gatcagttca 56340
agcagtttat gagtggagac cttagggatt tctctgtaca gtgtcatgtc atctgcacag 56400
aatgacaatt tcacctcttc gcttccatct tggatacctt ttatttcttc tgtttgtccc 56460
tttgctgagg ctaggacctc caatactatg ttgaatagaa gtggtgagac tgggcatcgt 56520
tgccttgttc gtaaaagaca gcaatggtgg taaggctttc ggcttttctc tgttaggatt 56580
aaagtggctg tgggtttgtg acaaatggct ttaatcttcg acaaaggagg caaggatata 56640
aaatggggaa aagacagtct tttcagcaag tgttgctagc taaaacggac agccgcatgt 56700
acatcaatga aactagaaca caccctcaca acaggcacgc acacacacac acacacacac 56760
acacacacac acacacacac tctatatggc ttaaagactt aaacaagaca taacaaaact 56820
cttagaagag aacacaggca aaacattttc tggcatcaac cacacaaatg atttcttagg 56880
tcactctccc aaggcaacag aaacaaaacc aaaaataaac cgatgggatg taatcaaacg 56940
tacaaccttt tgcacagcca aggaagccgt taaagaaaag acaacctacg gaataagaga 57000
aaagagtttc tgacaatgca accgacaagg gcttagtctc tgaaatatac aaacagccta 57060
cacaactcaa cagcaagaaa caccacccaa ctgaaagacg ggcagaagac ctgaatagac 57120
atgtcgccaa agaagacaaa gagatggcca acgggcacac taaaaagtgc tcaacatcac 57180
tgttcattag agaaacgcga ctcaaaactg ctatgaggta ccaactcaca cctctcagaa 57240
tggccatcat gaaaaagccc acaaataaca aatgatggaa ggggtgtgga ggcaagggaa 57300
ccctcctgca ctgctggtgg gaatgtaaac tggtacaacc actccggaaa gcagtatgga 57360
ggtacctcag gaaactaaat gtagaactac catatgaccc agcaatccca ctcctgggga 57420
tatatccaca caaaactttc cctgaaaaag acacatgcac ccatgtgtgt tcactggagc 57480
actagtcacg ctcgccaaga catggaaaca acctaaatgt ccatcgacag gggaatggat 57540
taagaagatg tggtatatat acacactaga ataccactcg gccgtaaaaa cgaaccaaat 57600
aatgccattg gcagcaacac ggatggaacc agagactctc atactaagtg aagtaagtca 57660
gaaagaccaa taccatacgc tatcacgtac atctggaatc taatatatgg cacaaataaa 57720
cctttccaca gaaaagaaac ccatggactt ggagaaaata cttgtggtcg ccaagggtga 57780
ggtagtgggg tagactggga gtttggggtt agtagatgca acatattgca tttggcatag 57840
ataaactatg agatcctgct ggagagcaca gggagctgta tctagtccct tgtgatggaa 57900
catgttggag gataaagtga gaaaaagaac atataaatat atacaccaca cgcacacaca 57960
cacacatata tgaatgactg ggtcactttg ccgtagagta gaaattgaca gaacacagta 58020
aatcaactat aacggaaaaa aataaaaaac attaaaaaaa aaaaaacaga tgctcatctc 58080
catggattct gaacacattt ttgacagaat tcaacaccca actttttctt gatgattttt 58140
attttttcca ctgcaggtgg tttaccctgc tctgttgatt ccactgtaca gaaaagtgac 58200
aaagtcacac acatatatat atgtagacat tctttttctc acattatcct ccatcatgcc 58260
ccatcatagg tgactagata gagggaaact acaccaacat actaaaagcc atatatgaca 58320
aacccacaac tatcatcatt ctcaatggtg aaaacctgaa agcatttccg ctaagatcag 58380
gaacaagaca aggatgtctg ctctcaccac tctccttcca cggagttttg gaagtcctag 58440
ccagggcaat tagagaagaa aaggaatcca aatcggaaac ggagaagtaa cactaccgct 58500
ctttacaggt gacgtgctgc gatacctaga gaatcctcaa ggcactacca gaaacctctt 58560
agagctcgcc aatgaatttg gtcaagctgc aggatacaaa attaacacac agaaattggc 58620
tgccttgctc tacgttaaca atgaaagagc agcgagagaa actagggaaa ccatcccact 58680
gagcagtgcc tcgaaatgaa tgaaatgccc aggaataaac ctacccaaag acacaaagac 58740
ctggactctg aaaaccataa ggcactgctg gaagcaacca aagacgacac aaaccggtgg 58800
aaagctagac catgctcctg ccctggaaga attaatactg gcaaaagggc cataccacct 58860
aaggccaccc tcactctcag tgcaatccct ctcaaatctc caatggcatt ttccacagaa 58920
ctagaacaaa aaacctcaaa aatttgcatg gaaacacaaa tacctcactg acctgggcta 58980
tactacaaag ctgcagtcat caaaacggta tggcactgac actccccccc aaaaaagaca 59040
tacatatcag tggaacagaa cagaaagccc agcaataaac ccaagcacct atgctcaatc 59100
catccatgac ataggaggca agactattca acagagaagg gagagtcact tcactaagtg 59160
gtgctgggga gctggacagc tccatgtaaa gaatgaaacc agaacactcc caaacgccaa 59220
acacaccaaa aaaaaacaaa aaacaaaaaa aaaaaaataa aaaaaccctc aaaacaaatg 59280
aaactcctaa acgtaagact ggatacctat aactcacaga ggaaaacagg cagaacgttc 59340
tttgacataa atcacagcaa catcttattt gatccacctc ctggaataac tacaataaag 59400
acaaaagtgg agttcccgtc acggctcagt ggtaaccaaa tctgactagg aaccatgagg 59460
ttgcgggttc aatccctggc cttgatcagt gggttaagga tccggcgttg ccgtcagctg 59520
tggtgtaggt tgcagacgcg gctcggatcc cacgttgctg tggctctggt gtaggccgac 59580
agctacagct ccaattcgac ccctagcctg ggaacctcca catgccatgg gtgcggccct 59640
ggaaaagaca aaaagacggg gaaaaaatga aagaaagaaa gaaggaaaga aggaaaggag 59700
gaaagaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagac agacagacaa 59760
aagtaaacca atgagatcta attaaactca aaagcttttg aaaagcaaaa gaaaccattt 59820
taaaagagaa aaacaggagt tcccgtcctg gctcaggggt taacgaatct gactagcatc 59880
catgaggagg cagatttgat ccctggcctc actcagcagg ttaaggatcc ggcattgcca 59940
tgagctgtga gctgtggtgt aggccagcag ccgtagctcc aattcaaccc ctagcccggg 60000
aacctccata tgccacaggt ggggccctaa aaaccaaaaa agacaaagga aaacaaccca 60060
cagaatggga caaaatcttt gcaaacaatg cagcccacca aggctcaatc cccaaaatat 60120
acaaacactc tacaactcaa caacaacaaa aaacaaacca acaaacaacc caatcgataa 60180
atgggcagaa gacctaaata gacatttctc caaagaagac atacagatgg ccagaagaca 60240
catgaaaaaa tcctcaacag cactaatttt tagggaaatg caaatcaaaa ctgcaatgag 60300
gcaccacctc acactggtca gaatggccat cattaagagt caactaacag caaatgctgg 60360
agagggtgag gagaagaggg aacccgcctt cactgttggt gggaatgcaa cttggtacaa 60420
gcactgtgga aaacagtatg gaggaacctc agaaaactga acgtaaaacc acgattgaac 60480
ccagaaatct cacccctagg cctgtatctc gatacgactt tcagtcaaaa acttacatgc 60540
acaactatgg actttgcaga agggttcaaa atgggcagga catgaaaaca ctcaaacgcc 60600
catcaacaga cgaacggatt aagatgtggt tctcacacaa agtaagaact acgtctcagc 60660
cgtaaaaaag gaccaagtca cgccatttgc agcaacgtgg atggaactag agactctcac 60720
actgagtgaa gtaagtcaga aagagaaaga cagagaccac atgataccac ttatatgtgg 60780
aatctaaaat acggcgcgat tgatcctgtg gacaaaacag agacagagca tggtcatgga 60840
gagcagactt tgctttccag ggggagggag agaagagatt gacagggggt ttggagctgg 60900
tagatgcaac tgatcacatt gaaaacgagt aagtgatggg tcctactggg cagcacacgg 60960
aacagtgccc ggcctcttgc gttagaacgt gagagaggaa ggagagtgtg aaaagaaaaa 61020
gaaggtaaat gtatgtacag ctgtacaagg gtaattgaag gaacgttgta aatcaactct 61080
ggttttaaaa aaaaaaaaaa aaatttaaag aaagaaaacg ttctccagag gcagaacgag 61140
aaggtggaac ggccgacagg tctctgtcct gcagcggccg ctgacacgac cccaggacag 61200
cggaaatgag acccaggctg cctgtcgggc acacggcggc cgccccggca ccctggtgat 61260
cctgtgcagc acgaggtgcc cagtagaggg gtgggcaggg cagggcaggg cagggcagga 61320
cctggggacg cggccgcagc aggtgggctc cagccaggaa ggagccacga gtggggtggg 61380
gtctgctcgc tgggctggag gcagggaggg cacctcggct ccaacatccc agccggggac 61440
ccggccagag gaaggggctg cgaggatgtc ttccaagcat ctctttgctc ttggaaccac 61500
gtggcgaagc tttctgaaag cagaccagac tgcagcacca cggtttctat tgtgaaggga 61560
tttttccaga aggagtggga ccttgcgaac ggggatgtgg acacagggtg gcttctgatc 61620
aagcccaggg ccctgtgcag ccaagtctca tggtcacgcc gtcccggccg gaccagcctg 61680
ccccccacgc ggacccgggg aaggcagggg tcccatgacc agcacgagaa cgactgactc 61740
gccttgacct caagccacct ccaggaccag gggcagacgg cccggcacca cgaggaggag 61800
gcagagcccc gggcagggcc cacttgccac ttcacctcgc cagagagccg ggcagatggc 61860
ggcacgcgac tcttggggcc tgacggcccc gagcccagac gctctcgggg ctttggccag 61920
actcgcaggg ctgctgagcc aagcagaggc tacaaagctc cacgggcttc caggcaaccg 61980
ctagagacac gtgggcacag ctcgcagagc cggtgaccca cgggccagat gggaacctcc 62040
ctcctgtgcc ccctcaaggc gtctcccgga acggtggcct cccacggcgc tggacggggc 62100
tcggggcagg cccgaggcag cacggcgccc acaccgtcca ctggccgctt ggaaggattc 62160
agccaacacc accccaggcc cgctgcacag ccggggtggg ggctgagacg tccctcaggg 62220
gtccccagtc ccagcctgat gctgaagtta caggacggcc caggggcgcc caggggacag 62280
ggggagctgc ggccctccac gaatgaggac agagggtccc tgacccccac gcacggccgg 62340
caggtctcgg tccacgcaga gcagtgagcg gctgcccgcc agcccgctct cggccgggca 62400
ccaccttcac aacccctggg aactctgtcc ccacgtgggc agcacgggcc tgggtctggg 62460
cagcaggctg ggcccagcca cacacgcggg ggtcacaaga ggtgcccggt ggggtcgggc 62520
aaccaagggc tcacactagg ggcctggcac ccgggctgca gtaggccagg cggcataatc 62580
cccgtgggac atagagaacc acgacgtcct gagttcccgc accctcccca ccgctcaccc 62640
accccacaca cccacatgag ccgccagggc actgtctgga ctcccccagg ccctctggga 62700
cagaaagcaa ccagaagaaa agggaacttc aggaagcagc cgggccaccc ggtttcaatc 62760
ccattcttag tgtgcagggc tgtgggcagg aaggtcgtgc ctccagggac cagacaccca 62820
cccgaggaag cagggctgca ggcacagagc acagacacaa agagccacat gcacctgggg 62880
ccagagcagg aggggcccgg agcccggggc agcaggggag ccacctcctc ggcccccaca 62940
ctcggtgcgt ggggttcacg aagaccaggg gcaggggcag cggagggggg acgaggaggg 63000
gagaccccac agcagggctg cagccgaccc agcccaggga acacagcctg cacgcgggtc 63060
atgtcgcccg cccagcacag ggaccaggga gccctggcag gggcacgccc acgccaacag 63120
aggtggtgcc ccagaggcca gaggaggtcg gtggggccgc caggcctctg cggaacatga 63180
tgggctgggg tcagaggcct gagggatctg ctcggccagg ttgctggagg cacagggctg 63240
ggcaggggct gaggacgagg ccaggatgga caggtgggtc tgggcggagg actcagggaa 63300
gagacggaca cagcgcaggg gacaaagagg tcacctgggg ttgcagggtc ctgtcccggc 63360
tgccggagcc cgcttgagcc ccagggcagg tatgaaagca gtaactgtcg ggtcccgggg 63420
aaggagccag ggccacgggc agcaggtgag ccgggagcag agcaggcagg gcgggccggg 63480
ctcctgtgtg cccagagctg gcagcacagg gactgagcct aaagctccac aggccgcagg 63540
agggctgacg ggagccaggg gcctgagggc aggagcggga gccaggggct gggagctggg 63600
cgccagtgca gctgagccag caactagagg gagccaggga ccagacaggc tccaagggtg 63660
gtcgcggcga gaagagggtg agctcagaag ggtcaggagc ccctccggga ggggaccagg 63720
tgggacagag gctctgccag ggggacgcta cccagagagg gtgggcaccc agggctccgg 63780
gaacaaaggc cagccctggg aaagaaagga agccaggagt ggagctagac aaggctgagg 63840
gttcgggggc aggaagaggg gtggtgacac tgaggcccag gagccccagg gaaaggggca 63900
ggtaggctgc aggtgagcag ggaaccggga gggcggaaaa gctgtcctta gagctgctgg 63960
gaacacatag agctggtgca ggtgaggagg ggttgggagg gcaagggggc tgaacctgaa 64020
gctcctcgga actggtagag cttgtgcaga tgagcagggg ctgggagggc agggggcagc 64080
cctgggacca cctgagagct gtagatcttg ggcaggtgag cacaaactgg tagggcaggg 64140
gaagaacctg cagttcctgg gagcaggagg agcttgtgca ggtgagcagg ggctggaggg 64200
cagggggcta aacctggagc tattgggaag aggttaggct tgtgcaggcg agcagggggt 64260
gggagggcga gggtcagtcc tgggagccct ttcgagccaa tagagcccag gcaggtgagc 64320
aggggcttgg cgggcaggga gcatccctgg gatgagtttg tgcaggtgag cagggggtgg 64380
gagtgtaggg agcagtccta gcaactcctg ggagctggta gagcttgggc aggtgaacag 64440
gggctggaag tgcagggggc acatctggga ggccctggga gcaattagcg ctctggcagg 64500
tgagtagggg ctcggagggc agggagcagc cctgggagct ccttggagca ggtagagctt 64560
gggcaggtga gcagggggtg ggagggcagg gggcagtcct agcaactcct gggagcttgt 64620
agagcttggg caggtgagaa cgagctggta gggcagggga atgaacctgg agttactggg 64680
tcaggaggag catgtgcagg tgagcagggg ctggagggca gggggctaaa cctggagcta 64740
ttgggaagag gttaggcttg tgcaggcaag cagggggtgg gagagcaagg gtcagtcctg 64800
ggagcccttg cgagccggta gagcccaggc aggtgagcag gggctgggcg ggcagggagc 64860
agtcctagca actcctggga gcaggtagag cttgtgcagg tgagcagggg gtgggagggt 64920
agggagcagt cctgggaact cctgcgagca ggtagagctt gtgcaggtgg gcaggggtgg 64980
gagggcaggg ggctgaacct ggagctcctc agaactggta gagcttgtgc agatgagcag 65040
tggctgggag ggcagggggc agccctggga gcaatagacc ttgggcaggt gagaacgagc 65100
tggtagggca gtggaatgaa cctggagtta ctgggtcaag aagagcttgt gcaggcaagc 65160
aggggctgaa gggcaggggg ctaaacctgg agctattggg aagaggttag gcttgtgcag 65220
gcgagcaggg ggtgggaggg caagggtcag tcctgggagc ccttgcgagc cggtagagcc 65280
caggcaggtg agcaggggct gggagggcag ggagcatccc tgggacctct tgggagcagg 65340
tagagcttgt gcaggtgagc agggggtggg agggtaggga gccgtcctag caactcctga 65400
atctggtaga gcttgcggag gtgaacaggg gctgggagtg cagggggcag tcctgggagc 65460
ccctgggagt aattagagct tgggcaggtg agcaggagct tggagtctag ggggctaaac 65520
tggagctatt gggaagagtt taggcttgtt caggtgagca aggggtggga gtagaggggg 65580
cattcctagg agctcccgcg agttggtcaa tctcgggcag gtaagcaggg gctcagaggc 65640
ctggagcagc cctgggacca tctgggagca gggcgagctt gggcaggtga gccgggccag 65700
gggggatgca ggacagggca ggggcaacag agtgggggtg agctcagggg agccccaggc 65760
tcgagggagg ggccgatata gggctagcca ggctggaaag tgggctcctg gggggaaggt 65820
ccccaggact gcgggggcag gggcagggct gaacagacgc catgggtcac agggatcggg 65880
ccaacgggcc ataccctgtt ccagcaaagt gagtggacat gggacagagc agctggagtc 65940
ctaagtgaga acagcggcac agggcagtgc acatggtcgg ggggtcgagg ggctcatctg 66000
ggcctgagct ggggaagcag gcaatggctg aacagaacag gggggagagg accggcccaa 66060
ccgggcaggg gcgcatgggg tgctggcgcc ccgtgggaga ggaaggccag gggcagggct 66120
cggctgcagc agtgctgcca ggcaggctgg cagcgggaag ggcaggagca gagggagggc 66180
tctggcctca gcaggcagct gggaggccca gagctgggct gcaggggccg gggctctggg 66240
gaaagctgcc tcgggtgagc tcagggccaa gccaggagcc ccggtggagg agggaccggt 66300
gggcccagag gcccagattt ggagcctggg ccagggccgg agggatggca ggggaggggc 66360
ctcggaggag actacctgcc ctcgcccagg gaaaggggca tgttggtgcc agggaccaac 66420
gcgcacgggc cttgactgca gagctgaccc agaggaacgg ggcctggaga gcaaacagca 66480
ggccaggacc aggggccagg agcagagcat gggcagtggg tggcagtggg tggcagtgtc 66540
cctgccaggc tggggaagca ggacggtggc ctcaggggac cagcagaagc tggccaggaa 66600
cacaggccac ccgctggtcg ggccaggcac ccacaggggc tctgggccct ggcgggctct 66660
catccaggcg gtcacccagc tctgaccagg cagctgcgcc cagaacctgc agctgcaatg 66720
gcagcgagct gggcggctgg tctgccgact ttctggaagc aagtgggtgc tgggcgcagc 66780
ggccccgctg ttctgagggc ccgattcgct gcccgcccca caaggaacaa ggccctggcg 66840
gtcacgcagc ctcctctctt ccagccccca agacggcccc atcggtctac cctctggccc 66900
cctgcggcag ggacacgtct ggccctaacg tggccttggg ctgcctggcc tcaagctact 66960
tccccgagcc agtgaccatg acctggaact cgggcgccct gaccagtggc gtgcatacct 67020
tcccatccgt cctgcagccg tcagggctct actccctcag cagcatggtg accgtgccgg 67080
ccagcagcct gtccagcaag agctacacct gcaatgtcaa ccacccggcc accaccacca 67140
aggtggacaa gcgtgttggt gagcgcctgc gctgggggcg ggagtgcacg tcgagacagg 67200
ccggggtgag ccccctgccc acagggacca gttcccagga tggagagtcc cgcccagggt 67260
gtcagggccg ctcacctctg tccccttcac ctgcaggccc aggctcaggg aggggtcaac 67320
tcggcacttc ggagaggcca gggtgggcac aggcgggacc cctcccccgg ccctgggccc 67380
ccagcagcac gagctgctct gggatgtacc aagacctgac cctgacctga gcccagccct 67440
gggccctccc cacccccagt gaccctgtgt gttctctcct caggaacaaa gaccaaacca 67500
ccatgtccca tatgcccagg taagaaactc gggcctcgcc ctcctcccca aggaggtagc 67560
ctgagcccag gttcggcccg aggggggaca ggtgcccagg aggaccccag gccacgagct 67620
gacccgcact ctgtctcccc caccaggctg tgaagtggcc gggccctcgg tcttcatctt 67680
ccctccaaaa cccaaggaca ccctcatgat ctcccagacc cccgaggtca cgtgcgtggt 67740
ggtggacgtc agcaaggagc acgccgaggt ccagttctcc tggtacgtgg acggcgtaga 67800
ggtgcacacg gccgagacga gaccaaagga ggagcagttc aacagcacct accgtgtggt 67860
cagcgtcctg cccatccagc accaggactg gctgaagggg aaggagttca agtgcaaggt 67920
caacaacgta gacctcccag cccccatcac gaggaccatc tccaaggcta taggtgggca 67980
aggggggcaa ggtgggcagg cggagagggt cccgtgggcc gcctgggggg accatcatgc 68040
taactgaggt gcctggcctc acagggcaga gccgggagcc gcaggtgtac accctgcccc 68100
cacccgccga ggagctgtcc aggagcaaag tcaccgtaac ctgcctggtc attggcttct 68160
acccacctga catccatgtt gagtggaaga gcaacggaca gccggagcca gagggcaatt 68220
accgcaccac cccgccccag caggacgtgg acgggacctt cttcctgtac agcaagctcg 68280
cggtggacaa ggcaagatgg gaccatggag aaacatttga gtgtgcggtg atgcacgagg 68340
ctctgcacaa ccactacacc cagaagtcca tctccaagac tcagggtaaa tgagccaccc 68400
gctgcacccc acgtgctctc gggtcccaag agctcgccta agccccagcg ctgtgtacat 68460
acgtcccggg ccagcatgaa ataaagcacc caacgccctc tagcacccgc aatgctgtca 68520
tggttctttc cgaggcagag ccccagtgca ggcaccaccc gcggggccag ggacaggaca 68580
ggctctgggc cagaggcgtc catgtggcat cggggggaga ggtcccacct gggccagacc 68640
cggtcggtgg aggtctgaac aggccccctg ggggctgccc gaggcaaagg agggagcttt 68700
gcctgaggcg gcctctcctg cgggctgccc tcctggcaca cctgctccgg ctgctgtgtg 68760
tggacggacg ggagaagccc tccaggcccc ctcggggaaa tgactgccca ggtcgtcccg 68820
cctctgtcca agcccaagct caggggcagc ggcccatcct tgtccccagg ttgcctgtct 68880
cctgggtctc ctgggtctcc tgtgcccaca gggtggggac ggccctgctc ctcccgcaaa 68940
cacccgacat caggccccca ccccgcctca ggccacgggg ctctgccctg ctggtccctg 69000
cagggggcca ggcctgggca ctcgtggcaa cactcacccc tgtgtcccac aggggcagag 69060
gtaggcagcc cagagtgtcc aggggaggga cagtcccaga cacccccagg agtggagccc 69120
agacccactg atatcaagcc cctcccaccg agatgcccag ctccccccca ccaccgcctt 69180
tctccactcg cacacccggg ctctcgcaca cgcatgcacg ccgcccccac ctcccagcac 69240
acccgcccac cgcctgcatc cacatgcaca cacacccggg cacacacaca cctacccacg 69300
cacacatgtg tacacccccg ggccctcaca gaccagagca tggagctggg tctcacacac 69360
cctcaccggg gacaccagcc cggccccatc ccccgaggcc ccacgggctg ctcagccaca 69420
ctggcacact agaccacacg gacccgctcc gacgcccggc cccgctcttc ccctggcacc 69480
tacgccccct ccggaacaag cagaaatccc cagcccgctt gtccccagca cagccgcccc 69540
cgcggcccgg ggacacacac cccaaggccg tctcggccct tccctgggcc agagcccacc 69600
gggcccctgc tcggctgacc ttccaccctc cgggcctggc cccctcggag agagggcggg 69660
gcctgagcca ctggcctgca cggtcaggac accccctccc agacggtccc catgctcggg 69720
ggcccccacc agaggagagg gggcaggaca tccctggcca ggctgagact cagcccagga 69780
tgcaggactg gggggccggg gccggggcca ggcactgagc tcagccgggg ggtcccccac 69840
ctggagccac cccaggcccc gcgggctggc acagcactga ccacccctcc ccgtgcagaa 69900
ctgctcctgg aggagagctg tgccgacgcc caggacgggg agctggacgg gctctggacc 69960
accatctcca tcttcatcac gctcttcctg ctcagcgtgt gctacagcgc caccgtgacg 70020
ctcttcaagg tgagcgggcc agccccgctg tcacccgtgc cccggagccc cctgccggcc 70080
cctccctggc cctccctgtg gccacgctgt cactgtgacg gcccctccct gtacccgccc 70140
tggcctctgg tcctctccgg gacacggtgg ctgtcgggga ccgcggggct ggcaggcgga 70200
ccctgggggg cggtccacgt gcggcccggg ccgccctcac cccgtgcgcc ctgcaggtga 70260
agtggatctt ctcgtcggtg gtggagctga agcggaccat tgtccccgac tacagaaaca 70320
tgatcgggca gggggcctag ggcgccctcc agggcggcca gggccgcccc agacccgaca 70380
ggaccgcgtc cgtcagctca agctgctccg cctgcgccag gcctgcctcc gccctctgac 70440
ctcgcccacc tctggctctc ggccacctcg cccgcacctc tgcccttggg catcgcggcc 70500
ccctcagaag gcgccccgca cccccaccgc ccctcggaag cccgagtggg cagcagctcg 70560
ggccccaacc tgacttgatg gacacccacc accaccagcc cccgccagag ccacgggaca 70620
ccgccagggc cacgtgtcct caccaggaac gcccggctca gccagcaggt ccagccggcc 70680
acgaggagac cggcgtccag cggctgacgg gacagggccc gggctccctg tggacaccgt 70740
ccagaccggg ctccagaggc agagtccccg agaggaggct cctgtgtcac agcctggagt 70800
gggaacagct gcccccaacg ccagcgtctc ccagcaggga agttcccaga cgggccacca 70860
acaggcgctc aggtcacctc cagcccaggg cactcgaggg aggaggacct gggcccgagg 70920
ccacggcagg agccccggtg gggacagagc ggccctgccc agggaggcgg tgaggtgccg 70980
ccagacaggc ctcctggagg gggtcccgct gactcggacc cccgcaggca ggacgggccc 71040
tcgctggaca caggcagggg tggaagggcc acccagggta cagggcagcc aacccatccc 71100
aaggtccgcg gcctgagtca cgtctatcgg accatccgtc catccagcag gagatccttc 71160
tgcctgcccc ggggcctggg tgagaggtga agaaccccca ggagcgagcc ccaggccccg 71220
gacccccaag gccgccccag aacggactct cccgccctgt ctctgcggga accccaagtg 71280
ggttcacggc atccggggag cagcctcggg actgggaggg aaggctcgtc tgccgagctg 71340
tggtcgctct ttctccttgg tgagtgtctg gagcccaggc actctcctca ctgttgctct 71400
catttttcac agcaataaac cacttttaca acccctcagc tcactgggac ctctgcctgt 71460
gtcacccgcc acccccccga atcccaacct ggccgccctc cccccacctc ccccccacag 71520
cgtttccccc caccaccccc aagcctcctg ggcacagcgg ccagcctggg gcctggcggg 71580
gccacgagcg actcgcatgt gttggctgtg tcatcagcct gtgctgctgt cgcaaacacc 71640
gcagccagtg gggtcgccgg acaaactgca ccaactcaga gctgagactt aggtcttcct 71700
ggggtgacat gaggccgcag ccggggaggc agcactgcag ggagctcgcc ggtaccgctc 71760
gctccaagca ggaggggact gaatgtcacc atgtgtgtga caggagggag ggcctgggaa 71820
ggccaggcca gggggtatcg ggggacagag gtcgggagaa gggctggccc agggagcaga 71880
gctggacccg gctcaagcgc tcaggacaga cgggtcaggg agaaggacgc ggggggtgca 71940
aaggggacct cgggcccaga cctccgcctg ggctacccag agggcctagc ccgtcccagg 72000
acaagccgag gggccgctcg gggctccggt gagggaccag ggagggggcc tggtggttgc 72060
gaccaggccg ccgtgtccgc cggccggctg acagccgtcc agggtgcggc caggactcca 72120
acctcccaga ggtgatcccg ccccagagaa acaggcctcg ggccagggct ggaggggaca 72180
ggactctccc aaaagggcag agcagggggc ccggctgccc gtgcttccta gtcacagctc 72240
caggaagggg gacagggcca gcccggggct gtcccaggga ggcatttgac gggagccggg 72300
tcacctgggg acatgacctg ggccactgaa tgctggcgct gaaagcctct cggggcgagg 72360
ggaggagccc caggccgagg ccagcaccac ggcttcccag gccaggagct gtggtcctgc 72420
tcagcctggc ccagagccca gggcatcggc aagggcccct ccagcgtccc gcagggaaca 72480
ggatggagac ggagccagag cagcatgcag ggaacggact gcgtcgctcc aggggtccct 72540
gaaccagaca cggggaggcc gggacgtgcg gccagagcat ggacgtgggg tggacagcag 72600
gggccaggca tgcagggcac ggagcgccct ccgcctccag gttctggggg ctcaggccac 72660
ggtcctgggg ctcaccccac accctccgtt tccctcaccc taaacgtcta gccaaacctg 72720
aacgtgccca tgggccacgc agcctgctca gccccaggcc ctggccctca cgtctgtccc 72780
cgcaccagcc atcagcccct ggccggccca ctcccgcggg accgccagca tggcgacagc 72840
ggggctggcc cccgcaccct cgagaactcc cccaccggac ccaggcctcc caccctgacc 72900
cccagcaacg cctcatccca cggcaacccc acctctccac gtgccccgcg caccccgagc 72960
ccagggcccg acgccagccc ctgagcccct cgggctcccc tgagtgagcc tgaagccaga 73020
cccccgcggc ctcctgaccc ctagaccccc agcattagct ttgcacactc cggtaccatc 73080
tcaccttgcc cacgcacacc cacatctgcg tcgtacacac acacacacac ccccacgggc 73140
acatggacgt ccaggatttc cagctcagcc ccaaagccga gggcactaag gggcccctcc 73200
gatcacagcc cccacgcccc cccaacttgg ggccactccc agaagagcgt ggctcccccg 73260
cagagagacc cccgctggac gtgggtgccg accacccatt cgcactgaac caccctcgaa 73320
acagagcgcg ggcccacggt ccagcccaca ctcagagccc accgccaaac cgagggctca 73380
gaggccaagg ccggcccacg gtccccgtgc accctgctcc ggagtccgaa gccagcccag 73440
ccccgccagg cctccctccc caggctcagc ccgcccctcc ctgcccccag ctcccagtgc 73500
ccgtgccccc cgtgcccagc ccagtccctg tgcccacccc cacccctgcg gtccaccctc 73560
cctgctggcc accccaggcc ccaggcctgc agaatgagga ggcagagaag ccttctgggg 73620
agaggccgtg ggaccctggg gctgtgggca aggccctcgg ggtgggaggg ggtcctcacg 73680
gaaggagctt cccggggggt ccacgcggcc gccgtctcgg cccagctgcc ccacccccac 73740
tcaccgtggc ctcgccgggg cctcgctcct ttcttttctt tggtttgttt ttgtttttgt 73800
tttgattttg gttgtttgtt tttatcccgc tacacagcat ggggctcaag ttcctcttac 73860
atgtatacac ttttcccccc accctttgtt ctgttgcaac gtgagtgtct agacatagtt 73920
ctcaatgcta ctcagcagga tctccctgta aatctcttct aagttgtgtc tgataacccc 73980
aagctcccca tccctcccac tccctccctc tcccgtcggg cggccacaag tctactctcc 74040
aagtccatga ttttcttctc tgtggaggtg ttcgtttgtg ctggatacta gattccagtt 74100
ataagtgaga tcatatggta tttgtctttg tctttctggc tcatgtcact caggatgaga 74160
gtctctagtt ccatccatgt tgctgcaaat ggcatgatgt ccttcttttt tatggctgag 74220
tagtattccg ttgtgtatgt acaccacatc ttccgaatcc aatcctctgt ggatggacat 74280
ttgggtggtt tccatgtcct ggctattgtg aatagtgctg caatgaacat gtgggtgcat 74340
gtgtctcttt taagtagagg tttgtccgga tacgtgccca agagtgggat tgcggggtca 74400
tatggaagtt ctatgtatag atttctaagg tatctccaaa ctgttctcca cggaggctgt 74460
accagcttac cttccgacca acagtgcagg agggttccct tttctccaca cgccctccgg 74520
cacgtgttat gtgtggactt cttaatgatg gccattctga ctggtgtgag gtggtatctc 74580
acggtagttt tgatttgcat ttctcttata atcagcgatg ttgagcattt ttgcctgtgt 74640
ttgctggccg tctgtatatc ttccttggag aacagtctat tcaggtcttt tgcccatttt 74700
tccattggtt gattggtttt tttgctgttg agttgtataa gttgcttgta tattctggag 74760
attaagccct tgtcagttgc atcgtttgaa gctatgttct cccattctgt aagctgtctt 74820
tttgttgtct ttggggtttc ctttgctgtg caaaagcttt tcagtttgat gaggtcccat 74880
gggtgtattt ttgctcttat gtctgttgct tggggagact gacctgagca aatgttcatg 74940
aggttgatgt cagagagtgt tttgcctctg ttctcttcca ggactctgat ggtgtctagt 75000
cttgtattta agcctctcag ccattttgag tttatttgtg tgcatggggt gacggtgtgt 75060
tctaatttca ttgctttgca tgccgctgtc caggtttccc agcaaggctt gctgaataga 75120
ctttcttttt cccagtttat gttcttgcct cccttgtcaa agattaattg accataggtg 75180
tcagggtttg tttccgggtt ctctattctg ttccctcggt cggtctgtct gttttggtac 75240
cagtaccaca ctgttttgat gacggtggct ttgtaatatt tcttgaagtc tgggagagtt 75300
atgcctcctg cttggttttt gtttctcagg attgccttgg cgcttccggg tcttttgtgg 75360
ctccatataa atgtttggat tgtttgttct agttgtgtgg aaaatgtcct gggtaatttg 75420
atagggattg cattgactct gtagattgct ttgggtagta tggccatttc tacactattg 75480
atttttccaa tccgtgaaca tggaatgtct ttccatttct ctacatcttc tttgatttct 75540
ttgattaaag ttttatagtt ctcggcatat aagtccttca cctcctcggt caggtgtatt 75600
ccgaggtatt tgattttctg aggtgcaatt ttaaaaggta ttgtattttg gtattccttt 75660
tctaatattt cattgctggc atacagaaac gcgactgact tccgaatgtt aatcttacat 75720
cctgctactt cgctgaattt attaatcagt tcaagcagtt ttggggttga gtccttcggg 75780
ttttctgtgg agagtatcct gtcgtctgca tacagtgaca gttttctctc ttctcttcct 75840
gtatggaggc cttgtatttc ttttgtttgt ctaattgctg tgcctgggac ttccaaaact 75900
atgttgaata gcagtgggga gagtgggcat ccctgtcttg ttccaaattt gagtgataag 75960
gcgttcagtt tttccccatt gagcattata tttgctgtgg gtttgtcaca aagggctttg 76020
attatgttcg ggaaggtccc ctctataccc actttggcga gggtcttgat catgaatgga 76080
tgttgtactc tgtcaaatgc cttttctgcg tctatggaga tgatcatatg atttttgact 76140
tttcctttgg taatgtggtg gatggcgttg attgattggc gtatgttgaa ccatccttgt 76200
gaacctggga tgaaccctac ctggtcatgg tgtctgattt tttggatatg ttgttggatt 76260
ccgttggcta agattcggtt gagaatcttt gcatctatat tcatcaaaga tattggccga 76320
tagttttctt ttttggtggt atctctggtt ttggaattag ggcaatggtg gcatcataga 76380
atgtcttcgg gagtattcct tcttcttcaa cctcttggaa aagtttaagg aggacgggca 76440
ccggttcctc tttatatgtt tgatagaatt cgcctgtgac gccatctggt cctggacttt 76500
tatttgtagg gagtgatttt atgacctctt caatttcatt tctagtgatc ggtctgttca 76560
gttggtctgt ttctgcttga ttcagttttg gcaggctgta agattctaga aaattggtca 76620
tttcttccag attgtcaaac ttgttgccat acagttgttc ataatattct cttatggttt 76680
tttatttttc tgctgtatcc gttgtgattt ctcctttttc atttataatt ttggttattt 76740
gggttctttc tctcctcttt ttagtgagtc tgaccagggg tttgtcaatt ttgttcacct 76800
tttcaaagaa ccagctcttg gttttattaa ttttctctat tgttttttga gtctctattt 76860
tattgatttc ttctttgatc tttataattt ccttccttct gctgacttta ggactttttt 76920
gttcttcttt ttctaattca tttaggtgga gggttaagtt gtcaatttgg gatctttctt 76980
ctttttggag aaaggcctgt attgctatga atttccctct gagcactgct ttcgcagcat 77040
cccatagatt ttgagaggtt gtgtcttcat tagcatttgt ttcaaggtag tttttaattt 77100
ccttcttgat ttcctcattg acctattggt tttttagtag catgttgttg agtctccatg 77160
cagtaggttt tttccctttt cttttcccat ggttgatttc tagtttcatg gcattgtggt 77220
cagagaagat acttgaaata atttctatgc tcctaaattt gtagaggtta gctttgtgtc 77280
ccaatatgtg gtcgattctt gagaatgttc cgtgtgcact tgagaagagt gtgtattctg 77340
atttttttgg atgtagtgtc ctgaagatgt caattaagtc taacttttct attgtttcct 77400
ttaggatctc tgttgcttta ttggttttct gtctatagga tctgtccatc ggtgtgaggg 77460
ggggtattaa cgtctcctac tatgattgta ttcccatcaa tatctccctt tatgtctgtt 77520
aatatgggtg ctcctatatt tggggcatac atgttgacga tagtaacatc ctctccttgg 77580
atagtaacat cctatccctt aatcattaag tagtgtcctt ctttgtcttt ctttatgtct 77640
tttgttttaa agtctatttt gtctgatatg agcattgcga ctcctgcttt tctgtcatgt 77700
ctgtcgacgt gaaatatttt ttcccacccc ttcactttca atctatacgt atcctttgtc 77760
ctaaggtgag tttcttgtag gcagcctatt gaaggttttt gcctttttgt ccactcagcc 77820
actctgtttc ttttgatcag agcattcggt ccattgacat ttaaggtgat aattgataga 77880
tgatcattta ttgccatttt gaacctcgtg ttccagttga tcctatggtt ctccattctt 77940
cctttctttt tttttttttt tttttttttt ttttcctccc ttggttggat ggtctctggc 78000
tattttctgc ttgagtgttt ttcttcattt tttgcgaatg caatgtttgg ttttgacttg 78060
tggttgccct gttttttaag tatgctaacc ccttcctata attgtgtgtt ttggcctgat 78120
ggtcctgtag gttcaaacac ttcattacta cactaaaatt aagaagagaa acaaataaac 78180
aaaccaaaaa gtgtctatta tttccttact tcccttgccc acattttatg attttgatga 78240
cttttttttt tctttttagc tttcttttgt ttgaaacttg ttcatgacta aatctgtatg 78300
ctggtttatt tgagtgactg ctctctgact gtggtttcct caatcctagt tctccctcct 78360
ttttttgttt ctgtttcctc ccttcctttc cttcctttct ttttggttta gagaagccct 78420
ttcagtattt cttttagcct ggattttgta ttgctgtatt cttttagctt ttgtttgttg 78480
gaaaaaaatt ttatttcccc tcctatttta aatgatgttc ttgctggata gagtattcta 78540
ggttgcattt tttttttctt ttagcacttt aaatatctct tgccattccc tcctggcctg 78600
tggagtttct gtatagaaat cagctgataa ccttatcggg gttcccttat aggcagcatt 78660
ctgcttttct cttgctgcct ttgggatcct ctctttatca ctaacttttg ccgttttcat 78720
tataatgtgt cttggtgtgg gtctctttgg gttcaacttg tttggggccc tctgtgcttc 78780
ctgtatctta aactcggtgt cctttagatt tgaagcgatc atttcttcaa atatattttc 78840
catcccctta tctttttcca ctccttctgg aacccctgtt atgcgtagat tggtgtgctt 78900
tatatcgtcc cataggtccc ttatattgcg ctcctgtttt tcctttggtt ttcagtctgc 78960
tgacctgatg gggtggtttc cgttattcta tcttccacat caccgattcg ttctcctgca 79020
ttattcgttc tggtttttac tgcccttaat tcagttcgta tctctgccaa tgaatgttct 79080
agtttttctt ggctcctcct tatattttcc agttcctttc tgagggtatc tgcattactg 79140
ttcctatctt ctcttcattc cttcagtatt ttcactattt ctcttttgaa ctccaggtct 79200
gtcagactgc agagatctgt ttcatcattg actgttttag gtgagttctc ctgttggttt 79260
gactgggggt ggtttctcag cttcttcatc ttgcttgttt tctttctcct ggtggagttg 79320
tactctccgt ggccagcagt gtttgccttg gcactgccgt ggaatgtttc ctgagggctg 79380
gcattgttag tcagtcttct ttgaggcagc gtggcatttc gagagggtgg gcggggctga 79440
cagggtctct ctgaagcaaa ggaggacttc ctgaggacag acaggactgg gaggtctgca 79500
ccgtgatgga agcagatgtc actcggccgg gcagagcttg ctctggtgtt tttgagctct 79560
ggggctcttg cagggggctg gcagtgctgg gcagctgttc catgggaatg cggcaccccc 79620
cgagggctgg agtggcgagc tgggcctctg agaacccaag aggctcttcc ccagggcagc 79680
ccaacttgga aggactgcct gagaacgtag gcagattttt cacggcccgg ccaagcttgt 79740
tctagctttt ctaggctgca ggctttttcc cgggggctgg cagtgtttgg tgctgctctg 79800
tgggggagta gcccctccag aggacaagaa ggccagggca ggggcagccc ctgcggtggt 79860
aggcttccgg caattattga ggcccaccct ccgggatggc aggcagcccc aggtccagcg 79920
cgtgcatgag gggtggcggt ggggggcggg gatgcactgg gaagcacagc tttctgatag 79980
ctggcaggcc agtgagctcg ctgtggtgtg gggagtattc catgggggca caccccttct 80040
ctcccctccc caacactgca cagacccagc tcttctccca gcttccctct gatttggctt 80100
tctgcttccc cacccttagc gtattgctcc ctccccccac aaagctctgc tttctagtct 80160
cccaggcagt ctctgcccca tcaaccttga cagaccggtt cctagacccc caagcagtct 80220
ccgcactgcc cacccatgcc cgttcccagg gactaacctc cggagctgag ttctcaatgc 80280
ccagccccca cccaagcgcc tcaatttttg gactgtgccc gtagttcaga tgatatgttt 80340
ggtactcgct ctgcttttca gaactctgac ttcctgctgc actctcctct gcatctggag 80400
agtcctccga ttcggctgat ctttccagcg agtaggtgac gttccagggt gtgggatccc 80460
tttctcctcc acagttccct ttcacgagcg cacatccagc tcggattccc cttctctcac 80520
cctcctcttt tctcttgttt tacccagtaa tgtcatgaga ttcttgtcat tattggaggt 80580
taggttcttc tgccagtgcc tgattgctgc ttaacctgta gatgggtgtg ttgttgttgt 80640
tgtgtttgtg ggaggggcga gcgtgtcccc ctactcttct gccatcttgc ctctgtctgt 80700
cttcctcact ccttgttagc gaagcccctt ttcccaagag cacggccccc gaaagacgtc 80760
caggcagggg cctgtgggcc tgtgttggtg ggaggtggcc ccccagcttg tggacaaggg 80820
gcaggggaag gggtccgagg acagtgctgg ggccctgagg gcgctcgtgt ccagacagag 80880
gctgcctcac gaggccctcc acaccctccc cccacccatc tgcaccccac ctgccccgct 80940
ggcctccagc gagagggcca gctggccaag gatgccccct cccctaaggg gaaggagctg 81000
gggggagccg cccgcagggt ctctgggaag tcctctggag gggggccggc gcttgggcgc 81060
ccgggacaga gggggcggct ccttcatcta ccaggagcac tgggccggca cggggaaggg 81120
agaagcctga gccctgggtc cttccccaaa gcacaagctg gggatctgtg caggtgggcc 81180
tggggcgccc tggggaccag tgcaggtggt ggtgccttgc accagggttc tctgtcaaag 81240
gggaggaatg ccttgggcat ctgtgtgggt gagggtggcg ttcactggga ctctttgctg 81300
gtggggtgct ggcaatgagg atcagtgcat gtgggctggg gtgcactggg tctcatctgt 81360
gcatttggtg gtggtatgca ctgcggagct atttcagcgg ggtggggcgc actgggtggg 81420
gatctctgct ggtggtgttt gtgtgcagtg cggatctgtg gtggtgaagg tgaggtacgt 81480
tgtttatatg tgaagttggc ggtggggtgc cttggggacc tgtgggggag gggcactagg 81540
atatgttcag gtgtcggtag catgcactgg gagtcttctc aactggaagt ggggtgccct 81600
ggaaatctgt gcaggttcag tggggtgccc tggggataag tgctggcaga ggtggggtgc 81660
cccgggaatc catgagggtg tggctggggt gccgagagga tcagtgcaag tggtggtaga 81720
gggcacagga tttctgtgca agtggaggtg gggtgtgctt gggatctaag caggggggtt 81780
gctccaatgg tcccgacggg gacctgtcca ggtgaatgta agctacactg gggaactgtg 81840
ggggcgaggc acactgggga tctgggaagg tgggggttgg gtgtactggg aatctaaatc 81900
tgtgggtggg gtgccctggg gacttgtgca ggaggatgag aggggcactc aggatctatg 81960
ggggtggggt gcactggcga tcgttgcagg tgggggtggg atggactagg aatcagtgca 82020
ggtagggtgg ggtgccctgg gaaaagatga aggtgggtgt gccgtgcatg gggatggcta 82080
catttgcagg ggtgcacagg ggatctgtgc aagtgggggt aaggtgcact agggatcttt 82140
gcaggtagag gcgtggagca ctggggatcc gggcaggggg aactggggtg cacaggagtt 82200
ctttccgggg tggggatagg atgaactgag cttctgtgcc aatggaggtg ggtgcctagg 82260
ggaccgttga agatgaggct gggatgccat gaggatctgt aagggtgtga gtaaggtaca 82320
ctggggatat ctagaggtgg gggtggggtg cactagggat gtaggcagac aggagcggtg 82380
gacctgaaga gctaaagggt ggcgtcacct gaggaactgt gcgagtgggg gaggctgccc 82440
tgggtatcag ggcggctggg gtgggggcac tggggatctg tgtgtgtgtt ggggggtgca 82500
ctgtggctct gcactgacgg aggtgggatg aacaggggat ttaccggagt ggggtgcact 82560
ggggatctgt gcaggtgcga tgggattccc agggaatcgg ttcaggagca ggtggctttg 82620
ccctggggat ctgtttgcat ggggtggggt gcactggtat tctgtgcaga agacatggcg 82680
tgcactaggg attcgtgccg gtggggttgg ggtgcactgg ggatctgcgc agttgcggcg 82740
gggtgcagtg gggatctgcg caggtgttgg agtgcactga ggtctgtagg gttggggggc 82800
actgagtagc tgtgcaagtg gggtgggaga tgcaccggga ccggaagtga gggggtgggg 82860
tgggctggga tgggagcagg tgggtggggt gccctgggga tcaggtaggg ggccttcgtg 82920
atcactggca ttggcagggc agggggtccg ggctctggcg agtgaatctg tacctagacg 82980
aaggccaaag cacacgtaaa tacatccaca cctctgactc tcagctgcag ccgcccgccc 83040
aggacacacg accaccatcc gcaggaacca gcccaggcgg cggctgcgcc ctctgcctca 83100
gacctgcctc tgtctacagg gacagcccgc cggacaggag gtgccctggc caccacacgg 83160
ggccgagccg cgctggccga cccgctgccc ctgccccacg gcctccatca ccaccccgac 83220
acgccctgtg ggaaggagcc ctctgcacgc gggaatcctt tgaaaggtcg gcgtacgcat 83280
cacacagggt gttcacctgt actgcctgtg cgcctaatgg gccctgctat gcacacgcca 83340
ccttcacagt aagagtgaac actcccaggc gtcggagacc cacggggcgg ccccgccagc 83400
gggtgggggc cgctccctgg gggctcagaa cacacggcct cggctttgcc ccgggaaaca 83460
cgtccagtgg agcaggagac acaggtgtcc ggcaacggct cggggccgtg gttgcaaacg 83520
gcgcccgatg catcctggga cctggaccca aggagggggc gagggggaag ctggccaagc 83580
cgcctgggca gggaggggat ggctcccgga ggcggaggtg agcagagaag ggggcggcgg 83640
ggccggcccg gggcctgagc agcgccgcgg gcggggagcg tcctgctggg accaccgctc 83700
tgccctgggc ccgccctcct cgtcctcaca ggactgacct caagccaggg ctgcccgcac 83760
ctgaagggca cgcagaccac cccaggagga gactccgggc ctttctcttt ttcgtttttt 83820
aatttcattt gactttattc tatattattt tatattatac cgttttaggg ccacacccac 83880
ggcatttgga aggtcccagc ctaggtgctg aaccagagcg gcagcagccg cctgcaccac 83940
agccacagca acaccagagc cgagcagcct ctgtgaccta caccgcagct cgcggcgacg 84000
cgtcctcatg gacgccagtc ggattcattc gcgccgcgcc atgacgcaaa ctcctctttc 84060
gcaatttctt ccattgaagg gtagttttac accgtcgtaa atttgccatg ttgtgttact 84120
ttcaggtccc cggcacagcg gcccggccac acacacacat atgcatccac acccacgtgc 84180
agacaggcag acacacgtca ggacacatac acagcggtgt gtggagaggt tgatcccaac 84240
ctcctggctc atctccccac ctctcgccct ggggaagcgt gcggtggccc catgtctgcg 84300
ggtccgtcgc ggttttgctt cgagtccact cgcacccctc cttttaatcc cacacgcagg 84360
caatgccgca cgatgtcggt ctccgtccgc gtcgcttcag ccagaggagc aggacgacct 84420
ctaggtccga cctcgtggtg gcaaatggca tcgccgcatc ctttgcacgg ctgagcgata 84480
acccgttgtg cctgtgcccc tcaccttctg caaccgtccg tccagacgga cgtttgggcc 84540
gcttcctgtc ttggtcattg cgaggagtgc tgcgacggat gctggagagc ccgtgcgttt 84600
tcacgttgtg gttctctccc actgtgtgcc cagcactggg gttgccgcat cggacggcag 84660
ttctgttcgc agtgctttca ggaagctcag gccgctttcc ggagtggtcg tacctacatc 84720
cccaccgaca gcgtaggggg ttcccttttc tccacaccct ctccagtata tacatcccgt 84780
ttgcagactt tttgatgatg gccactctgg ctggattgag gcgctacctc atggcagttc 84840
tgatttgcct ttctctaata attagcaaca gtgggcacct tttcatgcgt ttttggccaa 84900
ctgcacgtct gtcttctttg gagaaatgcc tgtgtaggtc ttttgcctct ttcttgatgg 84960
gattcttcat aatcaagctg catgccttgt ttgtatgttt tggagatgaa tcccttggcg 85020
gtcccttcat tggcaaataa tttctccctt tttgtaggtt gtctctttgt tttgcttgtg 85080
gtttcctttg cagtgccgca tatttttaag ttccgttaac tcctgtttgt tttattttct 85140
ttctatttcc attatgccag gagatggatc caaagagcta cagctgtggt ttctgtcaaa 85200
gagtgttctg cctattatcc tttgggattt ataggcatcc ggtcttatgt ttaggtcttc 85260
gattcctttt gagttgtgtg cgtgtgtgtc tggcgttaga gagtgttctg ctttcattct 85320
gttacgtgga gctgtccggt tttcctggca ccgatctttg aggagacttt ctcttctcta 85380
ttgactactc ttgccccttc gtcatggatg gattgacctg cctccgcttc cagcgatgta 85440
tatgtttggt tttaagccag tgccatactg tttgggtgac tgcagctttg tagtaaggtc 85500
tgaagtcagg gagcctgatt ccaccagctc catttttctc actttttcct cactcattgc 85560
gttggctatt tggggtattt tgtgtttcca tgcaaatttt tgaggttttt tgttctagtt 85620
ctgtggaaaa tgccattgga gatttgagag ggattgcact gagagtgagg gtggccttag 85680
gtggtatggc ccttttgcca gtattaattc ttccagggca ggagcatggt ctagctttcc 85740
accggtttgt gtcgtctttg gttgcttcca gcagtgcctt atggttttca gagtccaggt 85800
ctttgtgtct ttgggtaggt ttattcctgg gcatttcatt catttcgagg cactgctcag 85860
tgggatggtt tccctagttt ctctcgctgc tctttcattg ttaacgtaga gcaaggcagc 85920
caatttctgt gtgttaattt tgtatcctgc agcttgacca aattcattgg cgagctctaa 85980
gaggtttctg gtagtgcctt gaggattctc taggtatcgc agcacgtcac ctgtaaagag 86040
cggtagtgtt acttctccgt ttccgatttg gatgcctttt cttctctaat tgccctggct 86100
aggacttcca aaactccgtg gaaggagagt ggtgagagca gacatccttg tcttgctcct 86160
gatcctagcg gaaatgcttt cggctttgca ccgttgggaa tgctgttaac tgcaggtttg 86220
tcagacatgg cttttactct gttgatgtag tttcccacta gaggttttat caggaagggg 86280
tgttaatttt gtcaaaagct ttttctgtat ctgttgagat gatcagatgg attttgttgt 86340
tcggttggtc gattctcgga tattgaggga tctttgcatc cctgggataa agcccctcga 86400
tcatggcgag ggatctcttt attgtacatg tgaacgcagt ttgctaatat ttcgttgagg 86460
atttttccat ctatgttcat cggtgatgtt cacctgtcat tttctccttt tttttttttt 86520
ttttttttgg ttttttttgt ttgttttttt ttgttttggt atctttggtt tcggtatcgg 86580
ggcgatggtg ccttcacaga atgagtttga gagtgttcct ccccctgcaa cttttcggaa 86640
gcgcttcaga agaataggtg ttaactcttc tctggatgtt tgacagaagt catctgcgaa 86700
ggcgtcaggc ccttgaccta ggttttctgg aagtttttaa accacattct caatttcagt 86760
gcttgtcact cgtctgtttg tattttctag ttcttgctgg ttcaggcttg gtaggctgtt 86820
cccctgtaag aatcagtcca tttcctccgg gttgtcattt tatgggcaga cagtggctcc 86880
tactagtctc ttatgagatt tgtgtttcaa ttgcatctag ttgtcgtcac tgattttatt 86940
gaattgaatc ctgtcccttt tgtcccgatg agtctggcta aagtctcatg tgctgatgat 87000
acagctgcgg gtgcggtcac agaaaaggac aaaaagaaag gggaaaagaa agatttatcg 87060
atcttattag acttttccaa gaaccacctt ttggtttatt gatcttctct actgtttcct 87120
tggtcactac gtcattaact taagctctaa ggtctatgat ttccatcctt ctattgactt 87180
gcacctttga ctcttcttcc ttctataaat gttttagata caacattatg ctgcttattt 87240
gacttccatc tcctttccag agaggtgatt gtattgctag gacatttcac ttgttgccgt 87300
gccagtccag ggtgaatcca ggaaacataa gcccctctag gggtttcaag tgagaagtga 87360
tgccacccag gaaatcaaag gtgtataaaa tcggcacgcg ggctggcagc atgcccacag 87420
gggagaaggt cgtcgaggct cgggggcccc cgggaagccc agggtcttct gcctcagagg 87480
gctcctcgga cttgtgcgtg ggggcatcag ggtctcagag aatgcccctc aacagaacac 87540
agtccatagg aggagcccgc cgttaatatg ctccaaggtg aaaagctgaa ctcatttcca 87600
ctaagatcag gaccaagact aggatggcca ccctcaccac atagttggcc gccgtgtgct 87660
caacatggta gtggaagtcc tagccccagc agtcagagaa gaaaaagaaa ctaacgccgt 87720
ccttttggaa gagaaacgta agactctcac tgtttgcaga ggacatggcg ctgcacgtag 87780
gaagtcctaa agataccacc agggaactgc tagagctcat cagtgcatta ggtaccgtgg 87840
caggatacaa actaatgccc agaaatctac cgcatgtcta cacactcgca acaaaaggcc 87900
agaaagggcg ggggggtggg gggataatca aacttatcaa tgcatccaaa agaataaaac 87960
acctatgaac aaacctacct aaagaggcaa aagacccgta ctttgaagac tagaagacgc 88020
tgatgaaagc aatcaaagat gacacaaaca gatggaaagc cagaccatgc tcctggattg 88080
gaagactcag cattgtccaa atgaccgcag taaccatgca atctacacat ccaaccatgc 88140
catccctatc aaattgccag tggcattttg cacacaacta gaataaaaac ttcaaaattt 88200
atgtggaaca caatataccc ccaaagagcc aaagcaacct tgaaaaagaa aagtggagct 88260
ggtggaatca gcagttctag ttctgtgaaa gatgccgttg gaaacgtcat acgcggggcc 88320
cgaccacacg ctcatggggg cagaaggggt acccaccagg gccaagaggc caagggctca 88380
cacgaggggc ctggcacctg ggctgtggtg ggcaaggcag cataagccca gtgggacgta 88440
gagaaccgtg acggcctggg ttcccacgcc ctcccctggg tcccaggcac cactcaccca 88500
ccccacacac ccacatgagc cgccagggca ctgtctggac tccccccagg ccctctggga 88560
cagaaactac acagaaggaa agggaatttc aggaagcagc caggccaccc agtttcaatc 88620
ccattcttag tgtgcagggc tgtgggcagg gaggtcatgc ctccagggac cagacaccca 88680
cccgaggaag cagggctgca ggcacagagc acagacacaa ggagccactt gcacctgggg 88740
ccagagcagg aggggcccgg agcccagggc agcaggggag ccacctcctc ggcccccaca 88800
ctcggtgcga ggggttcacg aggactgggg caggggcagc tgtgggggga cgaggagcgg 88860
agaccccaca gcagggctgc agccgaccca gcccagggaa cacagcctgc acgcgggtca 88920
tgtcgcccgc ccagcacagg gaccagggag ccctggcagg ggcacgccca cgccaacaga 88980
ggtggtgcct cagaggccag aggaggtcgg tggggctgcc aggcctctgc ggaacatgat 89040
ggactggggt cagaggcctg agggatctgc tcggccaggt tgctggaggc acagggctgg 89100
gcaggggctg aggacgaggc caggatggac aggtgggtct gggcggagga cccagggaag 89160
agacggacac agcgcagggg acaaagaggt cacctggggt tgcagggtcc tgtcccggct 89220
gccagagccc gcttgagccc cagggcaggt atgaaagcag taactgtcgg gtcccgggga 89280
aggagccagg gccacgggca gcaggtgagc cgggagcaga gcaggcaggg cgggccgggc 89340
tcctgtgtgc ccagagctgg cagcacaggg actgagccta aagctccaca ggccgcagga 89400
gggcaggagc gggagccagg ggctgggagc tgggcgccag tgcagctgag ccagcaacta 89460
gagggaacca gggaccagac aggctccaag ggtggtcgcg gagagaagag ggtgagctca 89520
gaagggtcag gagcccctcc gggaggggac caggtgggac agaggctctg ccagggggac 89580
gctacccaga gagggtgggc acccagggct ccgggaacaa aggccagccc tgggaaagaa 89640
aggaagccag gagtggagct agacaaggct gagggttcgg gggccggaag cggggtggag 89700
atgctgaggc ctaggagccc cagggaaagg ggcaggaagg ctgcaggtga gcagggaact 89760
gggagggcag aagagctgtc cttagagctg ctgggaacac atagagctgg tgcaggtgag 89820
cagggggctg gcaggccagt ggggagatac ctggagctcc tgggaccacg gagagcctgt 89880
gcaggtgagc aggggttggt aggacagggg ctgaagctga aactctgggg agcaggtgta 89940
gcttgtgcag gtgatgaggg gctgggctga ggggtaggtt ctggaggtcc tgagagccga 90000
tagagcttgt gcaggtgaac agggcctggg agggcagggg ccacacctgg agcttctggg 90060
agcaggtaga gcttgggcag gtgagcatgg gctgagggca gggtgcagtc ctgggacctc 90120
ctgggagcag gcagagctcg ggcaggtgag caggggtggg taggacaggg ggcagtcctg 90180
gcagctcttg ggaacaggta gggcttgggc aggtaagcag ggtcaaggag ggcaggggac 90240
agacctggca gcgcatggga gcaggtagag tttctgcagg tgagcagggc ctgggagggc 90300
agggaacact cctgagagtg cctgggagcg ggtagagctt gggcaggtga ccacaggctg 90360
ggagtgcagg gggcagtcct gggacctcct gggaacaggt agagcttgtg caggtgagca 90420
ggggctggga gggtggggag cagacctagg accccctggg agcaggtaga gctcgggcag 90480
gtgagcaggg tctcggaggg cagcgagcag ccctgggacc tcctgggaac aggtagtgct 90540
tgtgcaggtg agcaggggct gggagggcgg ggaggagacc tgggaccccc tgggagcagg 90600
gcgagcttgg gcaggtgagc cgggccaggg gggatgcagg acagggcagg ggcaacagag 90660
tgggggtgag ctcaggggag ccccaggctc gagggagggg ccgatatagg gctagccagg 90720
ctggaaagtg ggctcctggg gggaaggtcc ccaggactgc gggggcaggg gcagggctga 90780
acagacgcca tgggtcacag ggatcgggcc aacgggccat accctgttcc agcaaagtga 90840
gtggacatgg gacagagcag ctggagtcct aagtgagaac agcggcacag ggcagtgcac 90900
atggtcgggg ggtcgagggg ctcatctggg cctgagctgg ggaagcaggc aatggctgaa 90960
cagaacaggg gggagaggac cggcccaacc gggcaggggc gcatggggtg ctggcgcccc 91020
gtgggagagg aaggccaggg gcagggctcg gctgcagcag tgctgccagg caggctggca 91080
gcgggaaggg caggagcaga gggagggctc tggcctcagc aggcagctgg gaggcccaga 91140
gctgggctgc aggggccggg gctctgggga aagctgcctc gggtgagctc agggccaagc 91200
caggagcccc ggtggaggag ggaccggtgg gcccagaggc ccagatttgg agcctgggcc 91260
agggccggag ggatggcagg ggaggggcct cggaggagac tacctgccct cgcccaggga 91320
aaggggcatg ttggtgccag ggaccaacgc gcacgggcct tgactgcaga gctgacccag 91380
aggaacgggg cctggagagc aaacagcagg ccaggaccag gggccaggag cagagcatgg 91440
gcagtgggtg gcagtgggtg gcagtgtccc tgccaggctg gggaagcagg acggtggcct 91500
caggggacca gcagaagctg gccaggaaca caggccaccc gctggtcggg ccaggcaccc 91560
acaggggctc tgggccctgg cgggctctca tccaggcggt cacccagctc tgaccaggca 91620
gctgcgccca gaacctgcag ctgcaatggc agcgagctgg gcggctggtc tgcggacttt 91680
ctggaagcaa gtgggtgctg ggcgcagcgg ccccgctgtt ctgagggccc gattcgctac 91740
ccgccccaca aggaacaagg ccctggcggt cacgcagcct cctctcttcc agcccccaag 91800
acggccccat cggtctaccc tctggccccc tgcggcaggg acacgtctgg ccctaacgtg 91860
gccttgggct gcctggcctc aagctacttc cccgagccag tgaccgtgac ctggaactcg 91920
ggcgccctga ccagtggcgt gcacaccttc ccatccgtcc tgcagccgtc agggctctac 91980
tccctcagca gcatggtgac cgtgccggcc agcagcctgt ccagcaagag ctacacctgc 92040
aatgtcaacc acccggccac caccaccaag gtggacaagc gtgttggtga gcgcctgcgc 92100
tgggggcggg agtgcacgtc gagacaggcc ggggtgagcc ccctgcccgc agggaccagt 92160
tcccaggatg gagagtcccg cccagggtgt cagggccgct cacctctgtc cccttcacct 92220
gcaggcccag gctcagggag gggtcaactc ggcacttcgg agaggccagg gtgggcacag 92280
gcgggacccc tcccccggcc ctgggccccc agcagcacga gctgctctgg gatgtaccaa 92340
gacctgaccc tgacctgagc ccaaccctgg accctcccca ccctcagtga ctctgtgtgt 92400
tctctcctca ggacgaccat gtcccatatg cccaggtaag aaactcgggc ctcgccctcc 92460
tccccaagga ggtagcctga gcccaagttc ggcccgaggg ggacaggtgc ccaggaggac 92520
cccaggccac gagctgaccc gcactctgtc tcccccacca gcctgtgaag ggcccgggcc 92580
ctcggccttc atcttccctc caaaacccaa ggacaccctc atgatctccc ggacccccaa 92640
ggtcacgtgc gtggtggttg atgtgagcca ggagaacccg gaggtccagt tctcctggta 92700
cgtggacggc gtagaggtgc acacggccca gacgaggcca aaggaggagc agttcaacag 92760
cacctaccgt gtggtcagcg tcctaccaat ccagcaccag gactggctga acgggaagga 92820
gttcaagtgc aaggtcaaca acaaagacct cccagccccc atcacaagga tcatctccaa 92880
ggccaaaggt gggcaagggg ggcaaggggg gcaggcggag agggacctgt ggggccgcct 92940
ggggggacca tcatgctaac agaggtgcct ggcctcacag ggcagacccg ggagccgcag 93000
gtgtacaccc tgcccccacc caccgaggag ctgtccagga gcaaactcag tgtaacctgc 93060
ctgatcactg gcttctaccc acctgacatc gatgtcgagt ggcaaagaaa cggacagccg 93120
gagccagagg gcaattaccg caccaccccg ccccagcagg acgtggacgg gacctacttc 93180
ctgtacagca agctcgcggt ggacaaggcc agctggcagc gtggagaccc attccagtgt 93240
gcggtgatgc acgaggctct gcacaaccac tacacccaga agtccatctt caagactccg 93300
ggtaattgag ccacttgctg cacccctcat gctctcgggt cccacgagct cacctgagcc 93360
ccagccctgt gtacatacat ccatggccag catgaaataa agcacccaac accaccctgg 93420
cacccgcaac gctgtcatgg ttctttccga ggcagagccc cagtgcaggc gccacccgcg 93480
gggccaggga caggacaggc tctgggccag aggcgtcccc gtgacatcgg gggcagcggt 93540
cccacctggg ccagacccgg tcggtggagg tctgaacagg ccccctgggg gctgcccgag 93600
gccgaggagg gagctttgcc tgaggcggcc tctcctgtgg gctgccctcc tggcacacct 93660
gctccggctg ctgtgtgtgg acggacggga gaagccctcc aggccccctc ggggaaatga 93720
ctgcccaggt cgtcccgcct ctgtctaagc ccaagctcag gggcagcggc ccgtccttgt 93780
ccccaggttg cctgtctcct gggtctcctg tgcccacagg gtggggacgg ccctgctcct 93840
cccgcaaaca cccgacatca ggcccccacc ccgcctcagg ccacggggct ctgccctgct 93900
ggtccccgca gggggccagg cctgggcact cgtggcaaca ctcacccctg tgtcccacag 93960
gggcagaggt aggcagccca gagtgtccag gggagggaca gtcccagaca cccccaggag 94020
tggagcccag acccactgat atcaagcccc tcccaccgag ctgcccagcc cccccccacc 94080
ccaccaactt cctccactcg cacacccggg ctctcgcaca cgcatgcacg ccgcccccac 94140
ctcccagcac acccgcccac cgcctgcatc cacatgcaca cacacccggg cacacacaca 94200
cctacccacg cacacatgtg tacacccccg ggccctcaca gaccagagca tggagctggg 94260
tctcacacac cctcaccggg gacaccagcc cggccccatc ccccgaggcc ccacgggctg 94320
ctcagccaca ctggcacact agaccacacg gacccgctcc gacgcccggc cccgctcttc 94380
ccctggcacc tacgccccct ccggaacaag cagaaatccc cagcccgctt gtccccagca 94440
cagccgcccc cgcggcccgg ggacacacac cccaaggccg tctcggccct tccctgggcc 94500
agagcccacc gggcccctgc tcggctgacc ttccaccctc cgggcctggc cccctcggag 94560
agagggcggg gcctgagcca ctggcctgca cggtcaggac accccctccc agacggtccc 94620
catgctcggg ggcccccacc agaggagagg gggcaggaca tccctggcca ggctgagact 94680
cagcccagga tgcaggactg gggggccggg gccggggccg ggcactgagc tcagccgggg 94740
ggtcccccac ctggagccac cccaggcccc gcgggctggc acagcactga ccacccctcc 94800
ccgtgcagaa ctgctcctgg aggagagctg tgccgacgcc caggacgggg agctggacgg 94860
gctctggacc accatctcca tcttcatcac gctcttcctg ctcagcgtgt gctacagcgc 94920
caccgtgacg ctcttcaagg tgagcgggcc agccccgctg tcacccgtgc cccggagccc 94980
cctgccggcc cctccctggc cctccctgtg gccacgctgt cactgtgacg gcccctccct 95040
gtacccgccc tggcctctgg tcctctccgg gacacggtgg ctgtcgggga ccgcggggct 95100
ggcaggcgga ccctgggggg tggtccacgt gcggcccggg ccgccctcac cccgtgcgcc 95160
ctgcaggtga agtggatctt ctcgtcggtg gtggagctga agcggaccat tgtccccgac 95220
tacagaaaca tgatcgggca gggggcctag ggcgccctcc agggcggcca gggccgccca 95280
gacccgacag gaccgcgtcc gtcagctcaa gctgctccgc ctgcgccagg cctgcctccg 95340
ccctctgacc tcgcccacct ctggctctcg gccacctcgc ccgcacctct gccctcgggc 95400
ctggcggcca cctcagaagg cgccccgcac ccccaccgcc cctcggaagc ccgagtgggc 95460
agcagctcgg gccccaacct gacttgatgg acacccacca ccaccagccc ccgccagagc 95520
cacgggacac cgccggggcc acgtgtcctc accaggaacg cccggctcag ccagcaggtc 95580
cagccggcca cgaggagacc ggcgtccagc ggctgacggg acagggcccg ggctccctgt 95640
ggaccccgtc cagaccgggc tccagaggca gggtccccga gaggaggctc ctgtgtcaca 95700
gcctggagtg ggaacagctg cccccaacgc cagcgtctcc cagcagggaa gttcccagac 95760
aggccaccaa caggcgctca ggtcacctcc agcccagggc actcgaggga ggaggacctg 95820
ggcccgaggt cacggcagga gccccggtgg ggacagagcg gccctgccca gggaggccgt 95880
gaggtgccgc cagaccggcc tcctggaggg ggtcccgctg tcttggactc ctgcggtcaa 95940
gatgggcccc actggacaca agcgggggta gacgggccac cccgggtaca gggcagccag 96000
accaccccga ggtcctcggc ctgagtcatg tccatctgtc catacgtcca tccagcagga 96060
gctccgacca caagccccag agctgggtga gaggtgagac ccccctgagc gagccacagg 96120
tcccggaacc cctaagcggg ccccccactg actctcccac cctgtctctg caggaacccc 96180
aagtgggttc acggcatccg gggagcagcc tcgggactgg gagggaaggc tcgtctgccg 96240
agctgtggtc gctctttctc cttggtgagt gtctggagcc caggcactct cctcactttt 96300
gctctcattt ttcacagcaa taaaccactt ttacaacccc tcagctcact gggacctctg 96360
cctgtgtcac ccgccacccc cccgaatccc agcctggcgt cctcccatga cctccgcccc 96420
tggcatcccc ccatgaattc ctccccccac ccccaagcct cctgggcaca gtggccagcc 96480
tggggcctgg cggggccacg agcgactcgc atgtgttggc tgtgtcatca gcctgtgctg 96540
ctgtcgcaaa ccctgcagcc ggcggggtcg ccgaaccaac tgcaccaact cagagctgag 96600
acttaggtct tcctggggtg acatgaggcc gcagcccggg aggcagcact gcaaggagct 96660
cgcaggtacc gctcgctcca aggaggaggg gactgaatgt gtgtgacagg agggagggcc 96720
tgggaaggcc aggccagggg gtatcggggg acagaggtcg ggagaagggc tggcccaggg 96780
agcagagctg gacccggctc aagcgctcag gacagacggg tcagggagaa ggacgcgggg 96840
ggttgcaaag gggacctcgg gtggagacct ctgcctgggt tgcccagagg gcctacccat 96900
cccaggacaa gccgaggggc cgctcggggc tccggtgagg gaccagggag ggggcctggt 96960
ggttgcgagc aggctgctat gtccgccgac cggctgacat ccgtccaggg tgcgaccagg 97020
actccaacct cccagaggtg atcccgcccc agaggaacag gcctcgggcc ggggctggag 97080
gggacaggac tctcccaaaa gggcagagca gggggcccgg ctgcccgtgc ttcctagtca 97140
cagctccagg aagggggaca gggccagccc ggggctgtcc cagggaggca tttgacggga 97200
gccgggtcac ctggggacgt gacctgggcc tctgaatgct ggcgctgaaa gcctctcggg 97260
gcgaggggag gagccccagg ccgaggccag caccacggct tcccaggcca ggagctgtgg 97320
tcctgctcag cctggcccag agcccagggc atcggcaagg gcccctccag cgtcccgcag 97380
ggaacaggat ggagacagag ccagagcagc atgcagggaa cggactgcgt cgctccaggg 97440
gtccctgaac cagacacggg gaggccggga cgtgcggcca gagcatggag gtggggtggt 97500
cagcaggggc caggcatgca gggcacggag cgccctccgc ctccaggttc tgggggctca 97560
ggccacggtc ctggggctca ccccacaccc tccgtttccc tcaccctaaa cgtctagcca 97620
aacctgaacg tgcccatggg ccacgcagcc tgctcagccc caggccctgg ccctcacgtc 97680
tgtccccgca ccagccatca gcccctggcc ggcccactcc cgcgggaccg ccagcatggc 97740
gacagcgggg ctggcccccg caccctcgag aactccccca ccggacccag gcctcccacc 97800
ctgaccccca gcaacgcctc atcccacggc aaccccacct ctccacgtgc cccgcgcacc 97860
ccgagcccag ggcccgacgc cagcccctga gcccctcggg ctcccctgag tgagcctgaa 97920
gccagacccc cgcggcctcc tgacccctag acccccagca ttagctttgc acactccggt 97980
accatctcac cttgcccacg cacacccaca tctgcgtcgt acacacacac acacaccccc 98040
acgggcacat ggacgtccag gatttccagc tcagccccaa agccgagggc actaaggggc 98100
ccctccgatc acagccccca cgccccccca acttggggcc actcccagaa gagcgtggct 98160
cccccgcaga gagacccccg ctggacgtgg gtgccgacca cccattcgca ctgaaccacc 98220
ctcgaaacag agcgcgggcc cacggtccag cccacactca gagcccaccg ccaaaccgag 98280
ggctcagagg ccaaggccgg cccacggtcc ccgtgcaccc tgctccggag tccgaagcca 98340
gcccagcccc gccaggcctc cctccccagg ctcagcccgc ccctccctgc ccccagctcc 98400
cagtgcccgt gccccccgtg cccagcccag tccctgtgcc cacccccacc cctgcggtcc 98460
accctccctg ctggccaccc caggccccag gcctgcagaa tgaggaggca gagaagcctt 98520
ctggggagag gccgtgggac cctggggctg tgggcaaggc cctcggggtg ggagggggtc 98580
ctcacggaag gagcttcccg gggggtccac gcggccgccg tctcggccca gctgccccac 98640
ccccactcac cgtggcctcg ccggggcctc gctcctttct tttctttggt ttgtttttgt 98700
ttttgttttg attttggttg tttgttttta tcccgctaca cagcatgggg ctcaagttcc 98760
tcttacatgt atacactttt ccccccaccc tttgttctgt tgcaacgtga gtgtctagac 98820
atagttctca atgctactca gcaggatctc cctgtaaatc tcttctaagt tgtgtctgat 98880
aaccccaagc tccccatccc tcccactccc tccctctccc gtcgggcggc cacaagtcta 98940
ctctccaagt ccatgatttt cttctctgtg gaggtgttcg tttgtgctgg atactagatt 99000
ccagttataa gtgagatcat atggtatttg tctttgtctt tctggctcat gtcactcagg 99060
atgagagtct ctagttccat ccatgttgct gcaaatggca tgatgtcctt cttttttatg 99120
gctgagtagt attccgttgt gtatgtacac cacatcttcc gaatccaatc ctctgtggat 99180
ggacatttgg gtggtttcca tgtcctggct attgtgaata gtgctgcaat gaacatgtgg 99240
gtgcatgtgt ctcttttaag tagaggtttg tccggatacg tgcccaagag tgggattgtg 99300
gggtcatatg gaagttctat gtatagattt ctaaggtatc tccaaactgt tctccacgga 99360
ggctgtacca gcttaccttc cgaccaacag tgcaggaggg ttcccttttc tccacacgcc 99420
ctccggcacg tgttatgtgt ggacttctta atgatggcca ttctgactgg tgtgaggtgg 99480
tatctcacgg tagttttgat ttgcatttct cttataatca gcgatgttga gcatttttgc 99540
ctgtgtttgc tggccatctg tatatcttcc ttggagaaca gtctattcag gtcttttgcc 99600
catttttcca ttggttgatt ggtttttttg ctgttgagtt gtataagttg cttgtatatt 99660
ctggagatta agcccttgtc agttgcatcg tttgaagcta tgttctccca ttctgtaagc 99720
tgtctttttg ttgtctttgg ggtttccttt gctgtgcaaa agcttttcag tttgatgagg 99780
tcccatgggt gtatttttgc tcttatgtct gttgcttggg gagactgacc tgagcaaatg 99840
ttcatgaggt tgatgtcaga gagtgttttg cctctgttct cttccaggac tctgatggtg 99900
tctagtcttg tatttaagcc tctcagccat tttgagttta tttgtgtgca tggggtgacg 99960
gtgtgttcta atttcattgc tttgcatgcc gctgtccagg tttcccagca aggcttgctg 100020
aatagacttt ctttttccca gtttatgttc ttgcctccct tgtcaaagat taattgacca 100080
taggtgtcag ggtttgtttc cgggttctct attctgttcc ctcggtcggt ctgtctgttt 100140
tggtaccagt accacactgt tttgatgacg gtggctttgt aatatttctt gaagtctggg 100200
agagttatgc ctcctgcttg gtttttgttt ctcaggattg ccttggcgct tccgggtctt 100260
ttgtggctcc atataaatgt ttggattgtt tgttctagtt gtgtggaaaa tgtcctgggt 100320
aatttgatag ggattgcatt gactctgtag attgctttgg gtagtatggc catttctaca 100380
ctattgattt ttccaatccg tgaacatgga atgtctttcc atttctctac atcttctttg 100440
atttctttga ttaaagtttt atagttctcg gcatataagt ccttcacctc ctcggtcagg 100500
tgtattccga ggtatttgat tttctgaggt gcaattttaa aaggtattgt attttggtat 100560
tccttttcta atatttcatt gctggcatac agaaacgcga ctgacttccg aatgttaatc 100620
ttacatcctg ctacttcgct gaatttatta atcagttcaa gcagttttgg ggttgagtcc 100680
ttcgggtttt ctgtggagag tatcctgtcg tctgcataca gtgacagttt tctctcttct 100740
cttcctgtat ggaggccttg tatttctttt gtttgtctaa ttgctgtgcc tgggacttcc 100800
aaaactatgt tgaatagcag tggggagagt gggcatcctt gtcttgttcc tgatctcagt 100860
gggaattctt tcagcttttc accattaaga atgatgtttg ctgtgggttt gtcatctatg 100920
gcctttttta tgttaaggta ggttccctct atgcccactt tctgaagggt ttttatcaga 100980
aatgggtgtt ggattttgtc aaaggctttt tctgcgtcta tggagaggat cacatggttt 101040
ttattcttca gtttgttaat gtggtgtatc acactgatgg atttgcggat attgaaaaac 101100
ccttgcatcc ctgggataaa tcccacttga tcatgatgta caatccttgt aatgtagtgt 101160
tggatgcagt ttgctagtgt tttgttgagg atttgtgcat caatgttcat cagtgaaatt 101220
ggcctgtagt tttctttttt ttgtggtagc ttttgttttg gtatcagggt gatgatggcc 101280
tcatagaatg agtttgggag tatcccttcc tctgcaattt tttgaaatag tttcagaagg 101340
agaggtgtta gttcttctct aaatgtttga taggattcgc ctgtgaagcc atctggtcct 101400
ggacttttgt ttgttggaag ttttttaatc acagtttcaa attcagttct tgaaattcag 101460
ttcagttcag ttcttcattc gtcttttcta tttcagcttg gtttagtctt ggaagattgt 101520
acttctctaa gaatttgtcc atttcttcta ggttttccat tttcttggca cgtagttgca 101580
tatagtagtc tctcatgatc cttcatattt ctgtgatgtc cgttgttact tccccttttt 101640
catttctaat tttgttgatt tgagtgctct ctctcttttt ctggataagt ctggctggag 101700
gtttatcaat tttgttgatc tcttcaaaga accagcttct catttcattg atcttttcta 101760
tggttttctt catttctatt tcattgattt ctgctctgat ctttgtgatt tctttccttc 101820
tactaacttt aggtcttgtc tgttcttctc tcttgagatg taaagttagc ttgtttattt 101880
gagctttctc tgtttatttt gaggtgggct tgtattgcta taaactttcc tcttagaacg 101940
acttttgctg catcccatag gttttggagt gttgtgtctt cgttgtcatt tgctgccagg 102000
tattttttaa tttcctcttt ggtttcttca gtgatccatt ggttgtttag tagcatgttg 102060
tttagtctcc atgtgtttgt gttttttgca gtttttttct tcttgttgat ttccagtcat 102120
atagcgtcgt ggtcggaaaa gatgcttgat atgatttcaa ttttcttaaa gttaaggagg 102180
ttggatttgt agcccaggat gtgatcaatg ttagagaatg ttccatgtgc acttgagaag 102240
aatgtgtatt ctgttgcttt tggatggaat gtcctataaa tatctattaa ctccatctgg 102300
cttaatgcgt cattcagggc ctgtgtttcc ttagtgattt tctgttctga tgatctgtcc 102360
attgctgtga gtggggtgtt aaaatccccc gctattattg ttattgtcaa tttctccttt 102420
taaggttgtt agtagttgcc ttatatattg tggtgaaact atgttgggtg aaaatggttg 102480
tatcttcttc ttggattgat cctttgatca ttatgtaatg accttctttg tcccttaaaa 102540
tattcctcat tttaaagtct attttgtctg atatgagtat tgctactcca gttttctttt 102600
gatccccgtt tgcatgaaat attttcttcc gtcctctcac tttcagtttg tatgtgtccc 102660
tagaagtgaa gtgggtctct tgaagacagc atatatatgg atcttgtttt tgtatccatt 102720
cagccagtct gtcttttggt tgggacgttt agtccattaa catttaaggt aattagtgat 102780
atgtatgttc ttattgccat tttattaatt gctttgaatt tgtttttgtt gctctttttc 102840
ttcccttctt ttcttgttct ctcctcttct ggtttgatga ctatctttag tgttgtattt 102900
gagttgattt ttgtatatca attgtagatt tttggtatgc tgttattctg aaattttgat 102960
ataagagtct atatgtatat gagattgttt taagctgttg gtctcttaat ttcaagttca 103020
tctctggtgt cctgcatttg tacccacttc ttctcatgat ttctgatttt ggtggcataa 103080
ttgtgcatgg aatgatttcg taccttttct gtatatatat ccctttactg gtaagccttg 103140
tctattgtgg tatttttgtt tcctgttgct ctcttttctt ttctgcctag agaagttcct 103200
ttagtatttg ttgtgagacc ggtttagtgt tgctgaattc tctcagcttt tgcttatctg 103260
tgaaggtttt gatttctcct tctaatctga atgagagcct tgctaggtaa agtaatcttg 103320
gttggagggt ttttcctttc atcatgttaa gtatatcatg ccactccctt ctggtctgca 103380
gagtttctgc tgaaaaatct gccaatgact ttatcggggt tcccttgtat gttatttgtt 103440
tcttttccct agcttctttc aacgttttct ctttgtgttt aattttggtc agtttgatta 103500
atgtgggttt tggtgtattc ctccttgggt ttattttgta tggtagtcgt tgagcttcct 103560
ggatttgagt gggtggttcc tttctcatgt tagggaagtc gtcagctatt acctcttgga 103620
atactttttc tgtccccttc tctctctctt ctccttctgg caccccaata ttacagacgt 103680
tggtgcattt aacgttgtcc caaagttctc ttgagactct cttcatttgt tttccatctt 103740
ttttctcttt tctgttccgc attcataatt tctactaatc tgtcctccac ctcgcttatt 103800
cattcttctg cctcctgtat tctgctgtta gctgcttcta gtgaattttt ttttcagtta 103860
ttgtactttg catctcttct tgtttaagtt ttatatcttg tatctctttg cttagtgttt 103920
cctataagtt atccaccttt gcatccagtt tatttccaat gttttgcatc atcttcagca 103980
tcaacaatct aaagtctttt tccaggaagc tgagaatatc ctcatcactt agctgatttt 104040
ctggggggtt ttctttctcc ctcatctgag ttatagttct ctgtcttttc atttttatag 104100
gtttttggtg tggtgacctt tttacagata atagagttgt agcatctctt acttctggtg 104160
gctgcccccc ttgtggttga ggttggtatg ggtgacttgc tgtagacttc ctgatgggag 104220
ggactgatgc ctgccccctg gtagatggag ctgattctaa tccctctggt gggtggggcc 104280
ttatctctgg atgggattag aggtggctgt atgcctgagg ggtctttagg cagtctgttt 104340
actcaggggc tgggctgtga tcccacctgg attgttgttt gccctggggt ttctcagcac 104400
tgatgggtag ggccaggttt ttccaaaagg gtcacctcca gagaaaggca cgctgatgaa 104460
gattcctgag agctttgctt ccaatgactt ccctcacaac aagccacatt cacccctgtt 104520
ttcccaggat gtcctacaag aactgcagtc aggtttgacc cagattccta tggagactta 104580
gctttgcccc gggacccagt gcaagtgaaa gtctgtgtgc accatttaag aatggggtct 104640
ccgtttcacc cagtcctgtg gagctcctgc gcacaagccc caatggcctt caatgccaga 104700
tgctccaggg gcgctttctc ccagtgccag agccccacac atgggagttt gatgtgggtc 104760
tcagaagtct cactcctggt aggtgagtct ctgtgaacca gttagtttcc agtctgtggg 104820
gcttcccaca tgggaggtat ggagttgttt atatcaccta ctcacccctc ctacctcttg 104880
atgtggcctc ctctttgtct tctggagtag gatatctttt tgaaggttta tggtccactt 104940
ggttgaagat tgctcagcca ttacttgtga attctgttgt ttttaggaga gaaattgaac 105000
tcaactgctt ttattctgca atcttactcc gatctccagg gaaccattgc gttacctaca 105060
ttactacctc tattccattt agtgtcagct gaaatggctc aaaagctggg agtggaatcc 105120
tctgagggct tgatctctca catgtctggc agctgatgct ggttgttaac tgagacatta 105180
gctggaagtg tcggctggaa caattacatg tgtaacgtgc ttcctctttt taggggttcc 105240
ctagaagcca ctaaatgttt taatacaagg ttatgttgtt tatttgagtt ccatctcctt 105300
tccagagagg tgattgtatt gctaggacat ttcacttgtt gctgtgcaag tccagggcga 105360
atccaggaaa cataagcccc tctaggggtt tcaagtgaga agtgatgcca cccaggaaat 105420
caaaggtgta taaaatcggc acgcgggctg gcagcatgcc cacaggggag aaggtcgtcg 105480
aggctcgggg gcccccggga agcccagggt cttctgcctc agagggctcc tcggacttgt 105540
gcgtgggggc atcagggtct cagagaatgc ccctcaacag aacacagtcc ataggaggag 105600
cccgccgtta atacgctcca aggtgaaaag ctgaactcat ttccactaag atcaggacca 105660
agactaggat ggccaccctc accacatagt tggccgccgt gtgctcaaca tggtagtgga 105720
agtcctagcc ccagcagtca gagaagaaaa agaaactaac gccgtccttt tggaagagaa 105780
acgtaagact ctcactgttt gcagaggaca tggcgctgca cgtaggaagt cctaaagata 105840
ccaccaggga actgctagag ctcatcagtg cattaggtac cgtggcagga tacaaactaa 105900
tgcccagaaa tctaccgcat gtctacacac tcgcaacaaa aggccagaaa gggcgggggg 105960
gggggggata atcaaactta tcaatgcatc caaaagaata aaacacctat gaacaaacct 106020
acctaaagag gcaaaagacc cgtactatga agactagaag acgctgatga aagcaatcaa 106080
agatgacaca aacagatgga aagccagacc atgctcctgg attggaagac tcagcattgt 106140
ccaaatgacc gcagtaacca tgcaatctac acatccaacc atgccatccc tatcaaattg 106200
ccagtggcat tttgcacaca actagaataa aaacttcaaa atttatgtgg aacacaatat 106260
acccccaaag agccaaagca accttgaaaa agaaaagtgg agctggtgga atcagcagtt 106320
ctagttctgt gaaagatgcc gttggaaatg tcatacgcgg ggcccgacca catgctcatg 106380
ggggcagaag gggtgcccac cagggccaag aggccaaggg ctcacacgag gggcctggca 106440
cctgggctgt ggtgggcaag gcagcataag cccagtggga cgtagagaac cataacggcc 106500
tgggttccca cgccctcccc tgggtcccag gcaccactca cccaccccac acacccacat 106560
gagccgccag ggcactgtct ggactccccc aggccctctg ggacagaaac cacacagaag 106620
gaaagggaat tccaggaagc agccaggcca cccagtttca atcccattct tagtgtgcag 106680
ggctgtgggc agggaggtca tgcctccagg gacccaacac ccacccaagg aagcagggct 106740
gcaggcacag agcacagaca caaggagcca cttgcacctg gggccagagc aggaggggcc 106800
cggagcccgg ggcagcaggg gagccacctc ctcggccccc acactcggtg cgtggggttc 106860
acgaagacca ggggcagggg cagctgtggg gggacgagga gcggagaccc cacagcaggg 106920
ctgcagccga cccagcccag ggaacacagc ctgcacgtgg gtcatgtcgc ccgcccagca 106980
cagggaccag ggagccctgg caggggcacg cccacgccaa cagaggtggt gcctcagagg 107040
ccagaggagg tcggtggggc cgccaggcct ctgcggaaca tgatgggctg gggtcagagg 107100
cctgagggat ctgctcggcc aggttgctgg aggcacaggg ctgggcaggg gctgaggacg 107160
aggccaggat ggacaggtgg gtctgggcgg aggacccagg gaagagacgg acatagcgca 107220
ggggacaaag aggtcacctg gggttgcagg gtcctgtccc ggctgccgga gcccgcttga 107280
gccccagggc aggtatgaaa gcagtaactg tcgggtcccg gggagggatc cagggccacg 107340
ggcagcaggt gagccgggag cagagcaggc agggcgggcc gggctcctgt gtgcccagag 107400
ctggcagcac agggactgag cctaaagctc cacaggccgc aggagggctg acgggagcca 107460
ggggcctgag ggcaggagcg ggagccaggg gctgggagct gggcgccagt gcagctgagc 107520
cagcaactag agggaaccag ggaccagaca ggctccaagg gtggtcacgg agagaagagg 107580
gtgagctcag aagggtcagg agcccctccg ggaggggacc aggtgggaca gaggctctgc 107640
cagggggacg ctacccagag agggtgggca cccagggctc cgggaacaaa ggccagccct 107700
gggaaagaaa ggaagccagg agtggagcta gacaagctga gggttcgggg gccggaagcg 107760
gggtggagat gctgaggccc aggagcccca gggaaagggg caggtaggct gcaggtgagc 107820
agggaaccgg aagggcggaa tagctgtcct tagagctgct gggaacacat agagctggtg 107880
caggtgagca gggggctggc aggccagagg ggagttacct ggagctcctg ggtcacggag 107940
agcttgggca ggtgagcagg ggttggagag cagggggaag agcctggagt tcctgagagc 108000
aggtaaaggt tgtgcaggtg aacttgggct gggtgtgcag ggggcagtcc tgggacctcc 108060
tgtgagcagg tagagctagg gcaggtgagc agaagctggg aggaaccagt tgaacctgga 108120
gcttcttgaa ccaggacgag cttgtgcagg tgagcgtggg ctgggagggc aggggccgca 108180
cctggagctt ctgagacaag gtagagcttg ggcaggtgag cagggtctgg gagggcaggg 108240
gacagtcatg ggacctcctg ggagcaggta gagctcgggc aggtgagcag aagctgggag 108300
ggcagggggc tgaacctcga gctcctggag gcggttgagc ttggggagct gagcaggggc 108360
tcagagggca ggggcttccc ctggaggtct tgggagcagg cgcagcttgt gtggttgagc 108420
agggggtggg agggcaggga gcagtcctgg cagctccttg gaagaggtag agcttgtgca 108480
ggtgagcagg ggctgggagg gcagggggta gtcctgagac ctcctgtgag caggtagagt 108540
tagggcaggt gagcagaagc tgggaggaaa cagttgaacc tggaacttct tgaaccaaga 108600
cgagcttgtg caggtgagca ggggctgcaa ggacaggagg cagtcctgag agatcctgga 108660
tcaggtagag cttgtgcagg tgaggagggg ctagaagggc agggaacacc aatgggagct 108720
cctgcgagca ggtagatttg ggctggtgag cacgagctga gagggcagtg gatgaacctg 108780
gagttcctgg gagcaggtag agcttgggca ggtgaacagg ggctcatagg gcagggggct 108840
aaacctggag ctattgggaa gtgattaagc ttgtacaggt gaacaggggt ggaatgcagg 108900
ggcaaacctg ggggcccccg caagctggta gagctcgggc aggtaagcag gggcatggac 108960
cgcagggagc agccctggca cctcctggga gcaggtggag cttgggcagg tgagaagggg 109020
ctggaggaat gcaggacagg gcaggggcag cagcagagag gctaagctca ggggagtcca 109080
ggctggaggg atgagctggt gcagggctag ccaggctgga aagtggaccc ctgggcggaa 109140
ggtcgccagg actctggaga caggggcagg cctgaacaga caccatgggc cacagggctc 109200
gggcccatgg gccccagccc tggtcgaaca aagtgagggg acataggaca ggacagctgg 109260
agtcctaagt gagaacagcg gcacagggca gtgcacatgg tcggggggtc gaggggctca 109320
tctgggcctg agctggggaa gcaggcaatg gctgaacaga acagggggga gaggaccggc 109380
ccaaccgggc aggggcgcat ggggtgctgg cgccccgtgg gagaggaagg ccaggggcag 109440
ggctcggctg cagcagtgct gccaggcagg ctggcagcgg gaagggcagg agcagaggga 109500
gggctctggc ctcagcaggc agctgggagg cccagagctg ggctgcaggg gccggggctc 109560
tggggaaagc tgcctcgggt gagctcaggg ccaagccagg agccccggtg gaggagggac 109620
cggtgggccc agaggcccag atttggagcc tgggccaggg ccggagggat ggcaggggag 109680
gggcctcgga ggagactacc tcccctcgcc cagggaaagg ggcatgttgg tgccagggac 109740
caacgcgcac gggccttgac tgcagagctg acccagagga acggggcctg gagagcaaac 109800
agcaggccag gaccaggggc caggagcaga gcatgggcag tgggtggcag tgggtggcag 109860
tgtccctgcc aggctgggga agcaggacgg tggcctcagg ggaccagcag aagctggcca 109920
ggaacacagg ccacccgctg gtcgggccag gcacccacag gggctctggg ccctggcggg 109980
ctctcatcca ggcggtcacc cagctctgac caggcagctg cgcccagaac ctgcagctgc 110040
aatggcagcg agctgggcgg ctggtctgcg gactttctgg aagcaagtgg gtgctgggcg 110100
cagcggccct gctgttctcg gggcccgatt cgctgcccgc cccacaagga ataaggccct 110160
ggcggtcacg cagcctcctc tcttccagcc cccaagacgg ccccatcggt ctaccctctg 110220
gccccctgcg gcagggacac gtctggccct aacgtggcct tgggctgcct ggcctcaagc 110280
tacttccccg agccagtgac cgtgacctgg aactcgggcg ccctgaccag tggcgtgcac 110340
accttcccat ccgtcctgca gccgtcaggg ctctactccc tcagcagcat ggtgactgtg 110400
ccggccagga gctcgtccag aaagtgcttc acgtgcaatg tcaaccaccc ggccaccacc 110460
accaaggtgg acctgtgtgt tggtgagtgc ctgggctggg ggcaggtgtg tagacgggct 110520
gaggtcagcc ttcctgccga gagaccaccc ctccagggtg tcagggccgc tcacctctgt 110580
ccccttcacc tgcaggccca ggctcaggga ggggtcaact cggcacttcg gagaggccag 110640
ggtgggcaca ggcgggaccc ctcccccggc cctgggcccc cagcagcacg agctgctctg 110700
ggatgtacca agacctgacc ctgacctgag cccaaccctg gaccctcccc accctcagtg 110760
actctgtgtg ttctctcctc aggacgacca tgtcccatat gcccaggtaa gaaactcggg 110820
cctcgccctc ctccccaagg aggtagcctg agcccaggtt cggcccgagg ggggacaggt 110880
gcccaggagg accccaggcc acgagctgac ccgcactctg tctcccccac cagcctgtga 110940
agggcccggg ccctcggcct tcatcttccc tccaaaaccc aaggacaccc tcatgatctc 111000
ccggacaccc caggtcacgt gcgtggtggt tgatgtgagc caggagaacc cggaggtcca 111060
gttctcctgg tatgtggacg gtgtagaggt gcacacggcc cagacgaggc caaaggaggc 111120
gcagttcaac agcacctacc gtgtggtcag cgtcctgccc atccagcacg aggactggct 111180
gaaggggaag gagttcgagt gcaaggtcaa caacaaagac ctcccagccc ccatcacaag 111240
gatcatctcc aaggccaaag gtgggcaagg ggggcaaggg gggcaggcgg agagggacct 111300
gtggggccac ctggggggac catcatgcta acagaggtgc ctggcttcac agggccgagc 111360
cgggagccgc aggtgtacac cctgtcccca tccgccgagg agctgtccag gagcaaagtc 111420
agcataacct gcctggtcac tggcttctac ccacctgaca tcgatgtcga gtggaagagc 111480
aacggacagc cggagccaga gggcaattac cgcaccaccc cgccccagca ggacgtggac 111540
gggacctact tcctgtacag caagctcgcg gtggacaagg ccagctggca gcgtggagac 111600
ccattccagt gtgcggtgat gcacgaggct ctgcacaacc actacaccca gaagtccgtc 111660
tccaagactc agggtaaatg agccacccgc tgcaccccac gtgctctcgg gtcccacgag 111720
ctcgcctgag ccccagcgct gtgtacatac gtcccgggcc agcatgaaat aaagcaccca 111780
acgccctcta gcacccgcaa tgctgtcatg gttctttccg aggcagaacc ccagtgcagg 111840
cgccacccgc ggggccaggg acaggacagg ctctgggcca gaggcgtccc tgtggcgtcg 111900
gggggagagg tcccacctgg gccagacacg gtcggtgggg gtctgaacag gccccctggg 111960
ggctgcccga ggcaaaggag ggagctttgc ctgaggcggc ctctcctgcg ggctgccctc 112020
ctggcacacc tgctccggct gctgtgtgtg gacggacggg agaagccctc caggccccct 112080
cggggaaatg actgcccagg tcgtcccgcc tctgtccaag cccaagctca ggggcagcgg 112140
cccgtccttg tccccaggtt gcctgtcccc tgggtctcct gtgcccacag ggtggggacg 112200
gccctgctcc tcccgcaaac acccgacatc aggcccccac cccgcctcag gccacggggc 112260
tctgccctgc tggtccccgc agggggccag gcctgggcac tcgtggcaac actcacccct 112320
gtgtcccaca ggggcagagg taggcagccc agagtgtcca ggggagggac agtcccagac 112380
acccccagga gtggagccca gacccactga tatcaagccc ctcccaccga gctgcccagc 112440
cccccccacc ccaccgcctt cctccactcg cacacccggg ctctcgcaca cgcatgcacg 112500
ccgcccccac ctcccagcac acccgcccac cgcctgcatc cacatacaca cacacccggg 112560
cacacacaca cctacccacg cacacatgtg tacacccccg ggccctcaca gaccagagca 112620
tggagctggg tctcacacac cctcaccggg gacaccagcc cggccccatc ccccgaggcc 112680
ccacgggctg ctcagccaca ctggcacact agaccacacg gacccgctcc gacgcccggc 112740
cccgctcttc ccctggcacc tacgccccct ccggaacaag cagaaatccc cagcccgctt 112800
gtccccagca cagccgcccc cgcggcccgg ggacacacac cccaaggccg tctcggccct 112860
tccctgggcc agagcccacc gggcccctgc tcggctgacc ttccaccctc cgggcctggc 112920
cccctcggag agagggcggg gcctgagcca ctggcctgca cggtcaggac accccctccc 112980
agacggtccc catgctcggg ggcccccacc agaggagagg gggcaggaca tccctggcca 113040
ggctgagact cagcccagga tgcaggactg gggggccggg gccggggcca ggcactgagc 113100
tcagccgggg ggtcccccac ctggagccac cccaggcccc gcgggctggc acagcactga 113160
ccacccctcc ccgtgcagaa ctgctcctgg aggagagctg tgccgacgcc caggacgggg 113220
agctggacgg gctctggacc accatctcca tcttcatcac gctcttcctg ctcagcgtgt 113280
gctacagcgc caccgtgacg ctcttcaagg tgagcgggcc agccccgctg tcacccgtgc 113340
cccggagccc cctgccggcc cctccctggc cctccctgtg gccacgctgt cactgtgacg 113400
gcccctccct gtacccgccc tggcctctgg tcctctccgg gacacggtgg ctgtcgggga 113460
ccgcggggct ggcaggcgga ccctgggggg cggtccacgt gcggcccggg ccgccctcac 113520
cccgtgcgcc ctgcaggtga agtggatctt ctcgtcggtg gtggagctga agcggaccat 113580
tgtccccgac tacagaaaca tgatcgggca gggggcctag ggcgccctcc agggcagcca 113640
gggccgcccc agacccgaca ggaccgcgtc cgtcagctca agctgctccg cctgcgccag 113700
gcctgcctcc gccctctgac ctcgcccacc tctggctctc ggccacctcg cccgcacctc 113760
tgcccttggg catcgcggcc ccctcagaag gcgccccgca cccccaccgc ccctcggaag 113820
cccgagtggg cagcagctcg ggccccgacc tgacttgatg gacacccacc accaccagcc 113880
cccgccagag ccacgggaca ccgccggggc cacgtgtcct caccaggaac gcccggctca 113940
gcgagcaggt ccagccgtcc acgaggagac cggcgtccag cggctgacgg gacagggccc 114000
gggctccctg tggaccccgt ccagaccggg ctccagaggc agggtcccca agaggaggct 114060
cctgtgtcac agcctggagt gggaacagct gcccccaacg ccagcatctc ccagcaggga 114120
agttcccaga cgggccacca acaggcgctc aggtcacctc cagcccaggg cactcgaggg 114180
aggaggacct gggcccgagg ccatggcagg agccccggtg gggacagagc ggccctgccc 114240
agggaggccg tgaggtgccg ccagacaggc ctcctggagg gggtcccgct gactcagacc 114300
cccgcaggca ggatgggcca tcgctggaca caggctgggg tggcatggcc acccgggatt 114360
cagggcagcc agcccatccc gaggtccacg gcctgagtca cgtctatcgg accatccgtc 114420
catccagcag gagatccttc tgcctgcccc ggggcctggg tgagaggtga agaaccccca 114480
ggagcgagcc ccaggccccg gacccccaag gccgccccag aacggactct cccgccctgt 114540
ctctgcggga accccaagtg ggttcacggc atccggggag cagcctcggg actgggaggg 114600
aaggctcatc cgccgagctg tggtcgctct ttctccttgg tgagtgtctg gagcccaggc 114660
actctcctca ctgttgctct catttttcac agcaataaac cacttttaca acccctcagc 114720
tcactgggac ctctgcctgt gtcacccgcc tccccccgaa tcccaacctg gccgccctcc 114780
ccccacctcc cccccccagc gtttcccccc accgccccca agcctcctgg gcacagcggc 114840
cagcctgggg cctggcgggg ccacaagcaa ctcgcatgtg ttggctgtgt catcagcctg 114900
tgctgctgtc gcaaacaccg cagccagtgg ggtcgccgga caaactgcac caactcagag 114960
ctgagagtta ggtcttcctg gggtgacatg aggccgcagc ccgggaggca gcactgcagg 115020
gagctcgcag gtaccactcg ctccaaggag gaggggactg aatgtgtgtg acgggaggta 115080
cctgtcgaac ctcccctgca gatcccagac tcctttcaaa tgtggctgtt gcagtccagg 115140
gcagggggac aggccaacta gggttcaaga gcttgcccaa ggcaaggcgt gaggaggacc 115200
agaccaggca aggggccaac agtgccatgg gacacaggca gaccctgaag gcacagtgcc 115260
caggacccag ccggcatgtg ggctctcagg cagctggttt gggcttctca gtgctgttct 115320
catcctgggg gcacgttaat ggctgtcctc ccagctcagg acccacctct ctctggctcc 115380
tgttccttgg gaagagggtc cacacctccc tttgggcctg cagtagccgc agggcatcca 115440
cggggtatcc acggcaagag ccgccactgc actgtcccct ccgctccgag aatggcacat 115500
tcccagaagc tcctgctcct ctggcctggt cccagaacag agaagccaga caaacggcag 115560
caggtcgggc agtgagggaa acacggcctg acgtcgaaac ccacccacct gggggctgtt 115620
agcacagccc caccaagcca aagctgccaa ctgagcacat gctcaactgc caccactgga 115680
caccaactct gcagcaagtg caaatgcagc aacaaccctt gctgtgcagg ttttcaggga 115740
caattttagc cagagttctc aagaaataga tgattgatag atagagaggt aggtaggtag 115800
atagataggt aggtagcaat aggaagaaga tgaggtacca ctgtagcaca tacctacgat 115860
agagctaaaa atgcatatac gtacacgtct agatagagct ataaatatag ctaaggccag 115920
ttacaggtat tgaggttaga gaaacagcca cgcagacgga gactgcagga cttctctcta 115980
ggagctggct ctcatgcttg cagggacttg gctggcaagt acgcgccctg gggggccagc 116040
aagtgggaac tcaggcagga gcatgtcctg ccgtcttgag gcagaatttc ttcctcttca 116100
gggaatcagt tttgttcctg acgccttcag ctggtgggcg agtggacact gtggagggtc 116160
cccttccttc cttaggccca tgcattgtag ctcttaaccg cattccaatc cctgcacagc 116220
agacgaagct caccctgagt ggcccagctg agcgggcacg tgagactccc gctcacaagg 116280
gcttcacttc cctctgtgct cacagcgccc aaggggcagg ggtccctgtc gcgatcttac 116340
cattgaagaa agtggtagaa agaaaaagaa ccccggcctg ggggaccctg gcctattgga 116400
cccccgcctg tgggagccct cccacgtggg accccacccg actgagggac ccctgcctgt 116460
ggggggacac caggcaggca tctacgtctt ctcacccgcg gcccacacaa cctcttagaa 116520
agactcctgc tccggtcctg gtccccggcc tcaggggcag gctgcccgag atgtccccac 116580
gcctgaagaa ggccagagac acggggcccg ggaagctctg tgaagatgca tctggggaca 116640
cccgcgtccc cgagggcaac tctgccgtca ccactgggca ctgagtaccc acggagagtg 116700
gcagacgtgg cctctggacc tcaaggaggc gagcgtccag ggaacaggaa ggcactgagg 116760
aaatcacgcc acagtgatgc gggcacggtc gtgagcaggg ctatgagtgg gaagctggca 116820
cggaggcccc acgacaggaa atacagctca gtctgcagct cctgcaggat cgggatccca 116880
ggagagcgcc ctgaggtgcg gctgcctcgg gtctgcggcc aacacccggt ctcccgcctc 116940
ccggccacag aaggtcctgc acacggccac gtggggccac gtgggagaag agagcgacag 117000
gccgaaggac gggtttcgag gcgcgatgtg atggggcggc gcccgtcgag gcatgaggaa 117060
tacatttccc agttttactc aacacaccaa tccatcagtt cagagtggaa ttcaacgtcc 117120
tacctgtcta ccgtggaaca aaggctgctg aaaggagtct atagattata aatggaaggc 117180
agggaagcat cactttcctt caaagaagag aatttgcatg gctctccatt atccacagcc 117240
tacagagtgg ggaagtaact caaaggcatg gaacgcgtgg agccccacgg aatcagacaa 117300
ccttgaaggg actgcaagac agggccttgc tgtccatgaa gacacaagaa gtaagctttc 117360
gatccacttc aaactctttt atacaatttc tcctacacac atgtacagcc gtcaaacaga 117420
tccagaatgt ataggaaact cctgcaaatc aattagaaaa accaaatgat tcagtagaga 117480
ataggcaaga tgcttcaaca ggtgtctcac aaaagatgcc caaacagctc ttggacattg 117540
gaaaggaacc acactggtta tgggggaaat gaaaatgaaa gaccccgcca gagaaagggc 117600
ctgcagcccc ggcagtggcg aggactaact tgggccggac taactccttg aaaatcgcag 117660
tgacgagccc tggaacaact gtttgaaata aaggcattgg tgaggggccg agagcaagta 117720
gacacaggag aagacagtcc ccttgaaaga tttgtctgag ggtgcacccc agtcctgccg 117780
ccggcgcagc tagaatgcaa gcaggatctg cggtcacacc tgctggaggg gtcaggtcac 117840
aggatgcagg actgcgcttc aagaactgct ccagactgtg gcttaaaacc gacggcttca 117900
ccagggtgtt cctgaaaagc cacagagtgg ttgagtcctt cagacccgcg gatcccgaag 117960
ccagactgga aaagggagtc tgctgcaggg ttgcgaccac gacggtctgt ctgtctccat 118020
cttctcttta gatcgggctg actgtgcgag ggaccctgga caaaacttcg cctgtttaga 118080
gtagccaggt ggaaagaagg accgccacgt ggggccgtgg ccttgccgcc gcggccgggg 118140
agcctgctct ggagccactg agcgcagggg cccgaggctt gggtttcatt tgttactaaa 118200
agcaagtcac tgcatttcaa agccttgaac tcaagcctca gcaccagctc gggtctgtct 118260
cagcccccag ggggtagctc aatggtgctt ttacaaagag gaggtgtggg ccaacaggaa 118320
gacgactcat ccaaaagatt tttaaatagg attgtgtaaa aaagagagcc gcggtggtgg 118380
cggccagctc ggcggggccg tgctcacgcg gccgcacgtt tgtagtgcag cgcaccgccg 118440
ggctcttcat tccggcgagg ggacagtctt cctgagagca gaggccacgc gcggtcaccg 118500
tcttgcactg tggtttgttg gcattttccc tccgtgtttg tgaagtttat ccggaatccc 118560
gaatcgcccg ggaggagccc ctgtctgccg cgtgtctgtc ggagccagac ttgggtcttg 118620
tttcctcgtg actccagaac agaaatgaca gctttttttc cacagccctg cggggtttgc 118680
gatctgcgaa tgccttgtaa aaagaagagt gcgtgtgtca ctgcatgaaa catctggtgc 118740
ttctaaatcc tccaagccgc ggtgagcaca gtgtattcag tgactggcac agaccacgtg 118800
agacccaggt tgcaagtcct gggtcttcaa cttcaagtgc aaagtctgtg aaaaccaatc 118860
tgagcctcgc attctcttaa atatttattt ttaacatatg aggtgttcca gagggttctc 118920
catttcaggg ctgcatggag gcgaaattca gcaataattt ctttcagtgg ttgtgaagtt 118980
gctttgtggt gccctccact gagccctggg cctttggcat aaaccccagt tttgtgggtt 119040
taggaaactc gtcatttttg cttacaaatt tacctttttg tattgtgcag tttaaatgtt 119100
ttggttgttt tccgttctgc gtaagtggct tgatgagaag acccctctag aacagcagcc 119160
cgtggtcagc gggacgggac acgggggctc tggcggctgc tggccacttg agggatgggt 119220
cttgttgcag tttgggcttg ttttgaggaa aagatcctgg agtcaattac aggttactgt 119280
agcagaattg ctttagggga attttgttta aaagaaaatg ggaccattct ggactttgca 119340
gtgacccctt ctccattttc tgaaaacgaa aatgatcagc tccgtgacac gccttttcag 119400
tgacaacgtc agcgttttat caaaaaccag aagttattag atgaaagcag ccattgattc 119460
tttcaaatat actcaatcat gcgcaaacaa gaagtatttt cttctcttaa actggaaatc 119520
tgtatcttta gaagcaatgt agcccaaaga tgtgaatctg aagaattttg ttgccaatgc 119580
tttatagcaa gtataagtga tctgagtgag taaaaagatg aaatagtctg aacgaaatgt 119640
gcactatatc agatttgaac atccagtgag actcacattc acactaagtt ttcaacattg 119700
tgttcttttg cattcgtttt ttactttcat tagaggttca aaaacaaaaa caaaacaaaa 119760
gcctacaaat taaggagaac cgtgcctaaa aggaggctcc gagccacaaa aggggaccct 119820
caagttctat atgtaaactc cacccaaatc cttgactctc cctttacctg tgcatgtgta 119880
gggaaaacgc caaagggcct ggggaaagaa caccagccaa gaggttgaaa gaactgcgat 119940
tttagctact gccaaccaca gcagtggtgc catggcagag aggttttgaa gtccagtcaa 120000
gctaactcct tctgacatag aattgatact tttggagttc ccgtcgtggc gcagtggtta 120060
acgaatccga cgaggaacca tgaggtttcg ggtttaatcc ctgaccttgt tcagtgggtt 120120
gaggatccgg cgttgccgtg tgctgtggtg taggtcgcag acgcagctcg gatcccgcat 120180
tgctgtggct ctggcgtagg ccgggggcta catctccgat tggacccctg gcctgggaac 120240
ctccacacac atgccacggg agcagcccaa gaaaaggcaa aaagacaaaa aaaagaattg 120300
atacttttgt gaggaagagt gcaaaatcca tagtctgtac tgcatttcaa ccacaacacc 120360
cagtgtacat ccaaaaactg ctaaatctgc aaaaaaaaaa aaaaaaaaga gaagaaggaa 120420
atgtgaccca tagtcaagat acaaggtaat caatggagag cagtcctcaa aggacccaga 120480
ctcgtgcccg ttatcaggaa agggcatcaa agcagctcgc aaagtatgtt ccagtttaaa 120540
ggagggtgtg ctcataagga gtgagcagct aatctctgca gaagatttta aacttcttac 120600
gagaaccaaa taaaaatgct agaactgcag agtacagttc cgaatttttt aaatcacttg 120660
atgagcctaa cagcagattg gagaaggcat gagaaaggcc agaaaactgt atttcaggac 120720
ataggcattc tgtatgtact acaggtattc taatgtgcta cgtgtgtaaa atacaactgc 120780
acgttatata gatatgacac actacctata ttttatgtat caataaaaat ttaaattcaa 120840
tccaaagaac agaaagtaaa atactgggga aaaaaatgaa aagagattcc atgtcctata 120900
ggacactatg aagcacttga aaatacgtga tattggagtt ccagaggggg acaagtgaga 120960
atggggcaga aaaagtcttt gaagaaacaa aaacctaggg tttcccaatg tggcagagat 121020
accagtttac agattgaaga aggccaccca gttcctaaca atgaatcgag ggaatcatgg 121080
aaattgaaac caaagtgaaa agaggttgtg acaacagcca gagaaatgac atgtacacac 121140
agggacaagt tacatctgac atcttatcag acaaaaaaaa tggtgaccgg gaaacaatag 121200
aaggaggaag gcttcctccc agaatcctgt ttacagagaa aacatctctc caaacggaca 121260
tgtaatatgt attggcagtt acacgaaaac tgaggaagtt tatttcaaac gggtctgcag 121320
taaaggagag gctttcctgc ctttcccgta accttgagca agaggactag ggaaagcgtg 121380
tcctaataga gaagctttgt ggcaatacct accttagcag agactctctg tgctgaaagg 121440
aaatgacacc ccatggaaac tcaggtctac agggtggaaa ggggagcatc agaggtggca 121500
aatgtgcaag tggaaaaatc gtcttttttc ccttactttc ttaataaaaa aaaaaaaagg 121560
atgattgcct aaagtaaaga ctacagctgg gttaatggag tttatttata acatgtatag 121620
acaccaagta tacaacaata gcacaaagta caaattcata aaggaaacaa tactttctcg 121680
aggttctaat aatttatttg aagcagtaag atattaaatc caaattcact gtgataactt 121740
caggatgtac actataggca cagacatcat aatgcaaaga ggcttatcta aaacatctat 121800
agacagagtt cctgctgtgg agcagtgggt taaaaatctg actgcagcag ctcaggtccc 121860
tgtggaggca caagttcgat ctttggccag gcacagtggg ttaaaggatc tggcattgcc 121920
ggagctgtgg cagctgcagc tcggattcaa tccctggccc aggaacttcc atatgccctg 121980
tttgggtgca gccataaaaa aataaattaa aagtcaatag agaaagtaga atggaatatt 122040
aagaagtgct gtttttaacc caaaagaagg taagaaagaa taaagctaca atgaacacac 122100
ctaatggata aactttaaca gtgctcccat aaagtcaggg acacagcgag ggtgcctgtc 122160
accgctgtca gagcttctgc agtacttttc agatatgtga ttggactcgc tgtggattgg 122220
gaaaccaaat cagagagtct cagccagtct gtttcaatca acaaaaacag aggagaaaac 122280
tccatgcact ctacataaca aagttaaaaa ttttcatgac aagtgtgttt tagtgacata 122340
agtatatgta ttacatgtgt tatatgtaaa taataggttg tgtgcactgg attatcacag 122400
aaaatacttt tttataagga ctatggtcaa aatggtttga aaaatggtca tttaacagat 122460
tatcggaggt cctagccaca gctacatgac aagaaaaaga aacagggctg ttaagactga 122520
aagagaaggt gtaaaataga cattatttgc agatgacgtg gtcgtttcca agagaaaatg 122580
acagaatcag cagcctctgg gaactaacaa gaaagtcagc aagatttcca tgcaaaacca 122640
acttaacaaa agcagttact cccaatgaga ttcctgtggg ttgggattgt ttgtttcttt 122700
gtctttctgt ctgtctgtga gatccactta cagcttagac cattttgaaa aataaaagca 122760
aaaacaaggg atttcaggga gactatcccc tgagtgttaa cacatacaaa cgtaataact 122820
gaaaaggatg gtactcgtgc tcaaactact agacccgagg aagaggagag cgctcagaaa 122880
cagcccgatt tctcctagga acccggtctt cgctgagtgt ggtgccatca ggcagcggga 122940
aagcttgagc cacgtcagag cctgttagga ttaaacactt acagttgaaa ggccaactct 123000
aaagctgaca ggaggtggac aagcctaggc tggtggagca cggctggcaa catctacctg 123060
gtggggtgtg cgtccaccca gaacttcagc acatgaccgt gtctggatac agggcttggc 123120
aaatgtaatt aggttaagga ttttgagatt agatcatact agatttcaga aaggttgtaa 123180
gtccagtgac ttataacaag aggaggagtt cctactgtgg taatacggga tggcagggtc 123240
actgcagcgc caagatccag atccaatccc cggccccaca ccgcagatta aggaaccagc 123300
attgccacag ctgtggctta ggttgcaact gtggcagaca tggtccctgg cccaggcact 123360
ccatgagctg tgggcaacca aaagaggaaa acatgaatga atgaatgaac gaattttttt 123420
aaaaagagac agaagtccag acggctccca ggtatctgaa gaggtgcccc agctcgttgg 123480
cagtggggca ctgggatgca agcagagaca cccgctgtgc cctacagagt ggcagccttc 123540
ccagagaaca ggacagcggc ttcctcgctg ggaaggcaga cacggagctg gaggcagggc 123600
ctccctggtg gagaggacgg caggaggccc gggaggcagc aggccagccc caggaagagg 123660
cgggtggcag tggggcaggc tggccccagg ggaacgaggc catgtggggg gactggagca 123720
cctgcggcca gtgtttgagg cgacagtcaa gggtaacgaa gggtcagctg gtctcactgt 123780
ccaggagaac tctggggcct ggaccccacc atgggacctg agggcagtga ttccctggct 123840
gagtctgagg gacatggagg ccagtgctgc tgcccagcta aaccctggtc agggaggtct 123900
aagaaccatc cgtaactggg ccttccctct aagcagccac tggccagacc ctgcacttgc 123960
ccctggcgac cccccaggtc cttacactgg ggcccagcct cagagggggc ttcaccctgc 124020
cttggctcaa agcccacccc tcccagggta tacagccagg tcccacctaa cctcctgcaa 124080
tccccaagcc ccccagctcc tggagagcct cctgaccacg caggatcctg ctctgggccc 124140
atctgagccg gacattaagg agcagccaga ggaagactcg gccttcgtgg tccaggcccc 124200
caccctgggc tgcgcctcgt cctccagccc tgtggggcag ccctgcccga tccaagcaca 124260
tgcagactca gcggcagccc tgttcttgcg cacgcagccc ccagccctcc tcagtcagca 124320
gggccctgag cgggctgtga gggcagaccc tctcccgaac acacacacac acacacacac 124380
acacgtgcgt acccacgggc acacgcactc tgagaaacat ggcactgaca gatatacgca 124440
cccagctgag acagcccccg tttagatgca gaatctgtcc caagtcactg aagtaaaggg 124500
gcagcactgt ggggccacag caggcagcta ggccctgagc tgggctctgg ccctggtgac 124560
acaggagagg ctgcaggcag gaccactgtg aaggtgggaa cagggaggca gggagggcga 124620
ggggactgtg cctgctggtg gaggggacag agagggagta gccaggccca tccctgggca 124680
agaggaaggc ggggggcaat tcctagaggc cagcgcaggg ccacagcccc agagaggaca 124740
gagactgtgt gtgcccagag ctcagtggga gaccagagcc agtctcctcc aacgctgcgg 124800
gaccccaggg ccccgaggct ggagaggccc atccgactcc cctcctttgc cccagcaact 124860
cagcacctcc cggctgctgg gggaggggct ggctgggcac cgaggcctct gccttgaaca 124920
cccacgactg ggcagaatga gcagggggag gccaagcccc cagcccggca tgctcacaca 124980
gggcaggccc caaggcctgg caccgcctgg tgggaggctt cccagttggg gctctggcag 125040
ccctgggttt ctctcataat cttgggtcct tccaggaaac tcggttttcc ccactccacc 125100
tcccacgcag cctctgtccc cacggcaggg tggcacagat caggactacc cgtgggcaga 125160
gaggtcaggc tgggccctgg ctccccgggc cctccatggc cctggccctg tgcactgggc 125220
tcaccccagc catctggggt ctcccaacgg agcccagacc tccctccaac ggtgccaggc 125280
cccacagtgc ccatctctcc ctggatgcag ggaacggggt cctggtgtca ccccctcaaa 125340
gaccacatgg tccttcccag gagcccctgc tggtggactg gcctggtaaa tccccatggc 125400
ctcccctgtg tgtgcacttg gcctgcaggg ggtctggaaa cacacaagcc tgcaggaccc 125460
gtccgcagca agaaggcaag gccaagactc agcccggcat ccagctcagc acaaggtagt 125520
ggaacagacc agagccaaaa tccgcaggcg cccctgtgaa ggccactctg cgggcaccac 125580
agctcctgag gatgccacag cacagacgtg gcatggcaca ccccgggctc tggtgggtac 125640
aagttcacag ccacatccag cctccccagg ggccctgcca gccctcactg gccacacagc 125700
tcagtcgcag ccctgatgcg agcccggcca taggacggtg actgggaagc cggcacacgg 125760
gcaccaggag gacgtgaggg gagcctgcct gcaccagctg gccccacagc cccacacgct 125820
ccaacacaga gccacccccg ggctggcccc agcccactcc tccgggcagc gcgatgcaga 125880
atgggggtgg cgtgccctca gggggcccca cgctcctcca tgaccgtttc ctcgcggggc 125940
tgcctcgggg gcagcggaga cagtgcctgg tgccctctgg cttcccaggc catggcgacc 126000
acactgtgtg cccgcggcac agcccgtcag cccgtggcat cgagagagag agagagagag 126060
agagagagag agagagagag gccatgaacc aggcggcaga agctggggac aaggccacag 126120
agaggggccc tgcctgagac ccgccatcac ccaatggact ttccaagaac tgagagaaag 126180
ggaatttcca aggctgccgg ggcccccggg ggttcccacc ccctttttag ccgagggcac 126240
tgaggcagag ctcccttctc caggccccgt ttctggacgt aaaaataaca gccatcatga 126300
cgatcaagtc tcctcacctg ggagccgtcc agggcgcctg agctggccac ggccggcagg 126360
atgggccacg ccggccacag gcgcctgaca tgggcacctc aggtacccct ccgccgggga 126420
gagctgccca ggctgcggct gggagagggc acctgggccg cccacctggt gtagacggag 126480
ctccttggtc accaacgtcg ctcagccgtc ccttcccggc gtgggcacac agggaggtga 126540
gagggtgctc agagccaagc tggagaagac caaggagcca agaccctcct ctgggccggg 126600
gcaggggtca cgcacagagt gtggacagtg ggctgggtgt ggatctgcct gcaaccagcc 126660
aggactgtca ctgtccacca gcccagggtc caagaggaaa gcagcagggt cagtccctgt 126720
cctggactcc acggcaggca ggcctgggct tggctggccc acactggcct gacgagggtg 126780
ggccaggcag ggctggactg ggccacagtg atcggggcca cgctggctag actgagccag 126840
atgaagtggg tttatagaaa tcctgtctca gtgaggtctg gtggcacgag ctgggctgtc 126900
ctggaccagg ctgatctggg gagatggggc aggaccaagc cgctccagct gagcagggct 126960
ttggtctcgg ccatcccagg gtggctcagc tgggcagccc taagctgccc tagactgacc 127020
cacgctggac tgcacttggc tcaacctagt tgggctgaaa caagagaccg ggatccgttg 127080
agaaggacca aaccagagta gctcagtggg atgacactgg gctgaactga gctgagtcag 127140
gctgaattgg gttgaactgg gctgaaatgg gttaagctgg gaaggctggg ctgggttgag 127200
ctgggctgag ctgcgctaag ctgaactggg ctgaactgcg ttgagctgaa ccagcttgag 127260
ctgtgttgca ctggactgag ctgtattgaa ctgggctgag ccgggctgag caggacagag 127320
ctgggctgaa ctgggctggg ctgtactaaa ttgggctgaa ctggactggg ctgggctgaa 127380
ctgggctgag ctgtactgaa ctgggctgaa ctgggctgag caggacagag ctgggctgaa 127440
ctgggctgag caggacagag ctgggctgaa ctgggctggg ctgtactgaa ctgggctgaa 127500
ctgggctgaa ctgggctgag ctgggctgaa ctgggctgag ctattctgaa ctgggctgaa 127560
ctgggctgaa ctgggctggg ctgggctgag caggacagag ctgtactgaa ctgggctgaa 127620
ctgggctgag ctattctgaa ctgggctgag ctcttctgaa ctaggctgaa ctgggctgag 127680
ctgggctgag caggacagag ctgtactgaa ctgggctgag caggacagag cggggctgaa 127740
ctgggctggg atgggctgaa ctgggctgag ctgggctggg atgggctgaa ctgggctgag 127800
ctgggctgaa ctgggctgaa ctgggctgaa ctgggctgag ctgggctgaa ctgggctggg 127860
ctgggctgaa ctgggctgag caggacagag ctgggctgaa ctgggctgag ctgtactgaa 127920
ctgggctgaa ctgggctgag ctgggctgaa ctgggctgag caggacagag ctgggctgaa 127980
ctgggctgag caggacagag ctgggctgaa ctgggctggg ctgggctgga ctgagctggg 128040
ctgagctgta ctgaactggg ctgaactggg ctgggctggg ctgaactggg ctgagctgta 128100
ctgaactggg ctgaactggg ctgagctggg ctgaactggg ctgggctggg ctgaactggg 128160
ctgagcagga cagagcgggg ctgaactggg ctgagctggg ctgaactggg ctgaactggg 128220
ctgaactggg ctgggctggg ctggactgag ctgggctgag ctgtactgaa ctgggctgaa 128280
ctgggctggg ctgggctgaa ctgggctgag ctgttctgaa ctaggctgaa ctgggctgag 128340
ctgggctgag ctgggctgaa ctgggatgag ctggactgag ctgggctgaa ttgagctgag 128400
ctgggataaa ctgggcaggg ctgggctgtg ctgaactggg ctgagctgag ctgagctggg 128460
ctgaactggg atgagctaag ctgagctgag ttgagctggg ctgaactggg cagaggtgga 128520
ctgagctgag ctaggctgaa ttgggctgag ctgggctgaa ctgacctggg ctaatctggc 128580
tgaactgagc tgggctgggc tgaattgagc tggcctggct gggctgagct gggttgcact 128640
gggctgagct tggctgggct gggctgggct gaactgggag gaactgacca gggttgagct 128700
gggctatggt gggctaagct ggttgaacca ggctgcactg ggctgagctg ggctgagctg 128760
ggctgcactg ggctgcgctg ggctgcgctg ggctgagctg ggctgagcta atcagctgag 128820
gttggagtgg ggggttgagt tatcctggcc agctttggga gctttgggag ctcaatcatt 128880
caattaaact gaaccatttt ctgcttgact gggctaggat gagttacttt ggggaaaaca 128940
ggagaccggg ggctgaacct tgccaggctg gtcaaggcag gttgtcttga tccaagctga 129000
cctgaaatgg gccgagctca acaggcaggg ctgggccggc cttgtgagct ggtttatctc 129060
ggctgttcta gcgcaaggtc agatgcgttg tgctcagccg ggctggatgg gcagaaggga 129120
acagggcagg cagagctgag ctgggcagca gagccaggca gcagagccgg gaccagctgg 129180
ggtctggagc tctggggcca ggggctatgc aggtaggggt ggaagccaaa gaagccggct 129240
ggggcttgag gtgcgggggc ctgaggggag ctctgggcgt ctccatcctt tctccacggg 129300
gccggtagtt gggctcaggg agcgggggtg ggggtggcag tcaggctccc agtggggggg 129360
acgtgcagca gggcagagaa atcaccaggg gagccaggct gttggggaaa aggacgaggt 129420
aaagggacaa ccccgacaga gcagctcagg gccctgaagg actgacccac agacacaggg 129480
acagggccca gtgggtggca ggtggacctg cccacagcaa gctcctaggg agtttggagt 129540
gggagaggga cactcggggt tcctgggaag cggggcttct ggaggcccag gcccttatga 129600
agtctgtggg tggcagagga agggacagcg tgatggttcc tggggtttcg gggttcctga 129660
tccccagtgg ggtccatggg gagggggcag aggagaagcc tggtgattcc tagggggacc 129720
ctccacctca gggtgtgggt tggcacctcc actcaagagc cccatgccca gccactcagg 129780
gccaagggca ggagtcctga ccaagggcag tgatggcgac tgcttctgat gggggcagag 129840
cagttcactg gagggcaccc cggggcgacc gggaggtaag gatagggcca gagccagccg 129900
cctgctccct gcgcctacgg caaaagtccc agcatcccct gcactggacc accctccccc 129960
cacaggcctc cctcccaggc cccccaggtc agcagttagc ctagcatggt gagtgacagt 130020
cctgaggcca gtgctgaccc tggccctgtc cccacaggag gg 130062
<210> 2
<211> 1475
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
gctagcgcta ccggactcag gagggcctat ttcccatgat tccttcatat ttgcatatac 60
gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag 120
tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 180
gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 240
tatatatctt gtggaaagga cgaaacaccg agctgcaatg gcagcgagct gttttagagc 300
tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt 360
cggtgctttt ttaccggtga gggcctattt cccatgattc cttcatattt gcatatacga 420
tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta 480
caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 540
tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 600
tatatcttgt ggaaaggacg aaacaccgag gaggctgcgt gaccgccagt tttagagcta 660
gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg 720
gtgctttttt ctcgaggagg gcctatttcc catgattcct tcatatttgc atatacgata 780
caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca 840
aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt 900
taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata 960
tatcttgtgg aaaggacgaa acaccgagcg ctgtgtacat acgtccgttt tagagctaga 1020
aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt 1080
gcttttttgg atccgagggc ctatttccca tgattccttc atatttgcat atacgataca 1140
aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa 1200
atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta 1260
aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata 1320
tcttgtggaa aggacgaaac accggacgta tgtacacagc gctggtttta gagctagaaa 1380
tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc 1440
ttttttctgc agtcgacggt accgcggtta attaa 1475
Claims (10)
1. A method of knocking out a porcine immunoglobulin heavy chain, comprising: the method comprises introducing into a porcine cell a substance that reduces or inhibits the activity or content of a protein of the IGHG region of a porcine immunoglobulin heavy chain constant region or a substance that modulates the expression of a gene encoding said protein;
the substance for reducing or inhibiting the activity or the content of the protein of the IGHG region of the porcine immunoglobulin heavy chain constant region or the substance for regulating the expression of the gene coding for the protein is any one of the following substances c1) -c 4):
c1) a substance that inhibits or reduces the activity or content of a protein in the IGHG region of the porcine immunoglobulin heavy chain constant region or a nucleic acid molecule that regulates the expression of a gene encoding said protein; the nucleic acid molecule is a DNA molecule of a gRNA expressing a protein coding gene targeting the IGHG region of the pig immunoglobulin heavy chain constant region or a gRNA expressing a protein coding gene targeting the IGHG region of the pig immunoglobulin heavy chain constant region;
c2) an expression cassette comprising the nucleic acid molecule of c 1);
c3) a recombinant vector comprising the nucleic acid molecule of c1) or a recombinant vector comprising the expression cassette of c 2);
c4) a recombinant microorganism containing c1) the nucleic acid molecule, or a recombinant microorganism containing c2) the expression cassette, or a recombinant microorganism containing c3) the recombinant vector.
2. The method of claim 1, wherein: c1) the nucleic acid molecule is a DNA molecule shown as b1) or b 2):
b1) the DNA molecule shown in the sequence 2 in the sequence table;
b2) and a DNA molecule having 90% or more than 90% identity with the nucleotide sequence defined in b 1).
3. The method according to claim 1 or 2, characterized in that: the nucleotide sequence of the IGHG region of the pig immunoglobulin heavy chain constant region is shown as a sequence 1 in a sequence table; the protein activity or content of the IGHG region for reducing or inhibiting the pig immunoglobulin heavy chain constant region or regulating and controlling the expression of the protein coding gene is to perform the following mutation on a pig genome sequence shown in a sequence 1 in a sequence table:
the nucleotide 1425 th to 111271 th in the sequence 1 in the sequence table in the pig genome is deleted.
4. The nucleic acid molecule of claim 1 or 2 under c 1).
5. An expression cassette comprising the nucleic acid molecule of claim 4.
6. A recombinant vector comprising the nucleic acid molecule of claim 4 and/or the expression cassette of claim 5.
7. A recombinant microorganism comprising the nucleic acid molecule of claim 4 and/or the expression cassette of claim 5 and/or the recombinant vector of claim 6.
8. A recombinant cell line comprising the nucleic acid molecule of claim 4 and/or the expression cassette of claim 5 and/or the recombinant vector of claim 6 and/or the recombinant microorganism of claim 7.
9. Use of any one of the following nucleic acid molecules according to claim 4 and/or the expression cassette according to claim 5 and/or the recombinant vector according to claim 6 and/or the recombinant microorganism according to claim 7 and/or the recombinant cell line according to claim 8:
A1) use in the knock-out of a porcine immunoglobulin heavy chain and/or knock-out of a porcine immunoglobulin locus;
A2) application in preparation of pig model with immunoglobulin heavy chain knockout and/or immunoglobulin locus knockout
A3) The application in establishing human immunoglobulin transgenic pigs;
A4) the application in preparing human antibody.
10. Product comprising a nucleic acid molecule according to claim 4 and/or an expression cassette according to claim 5 and/or a recombinant vector according to claim 6 and/or a recombinant microorganism according to claim 7 and/or a recombinant cell line according to claim 8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891834A (en) * | 2022-06-17 | 2022-08-12 | 吉林大学 | Method for preparing high-yield antibody rabbit based on CRISPR technology |
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| CN107208078A (en) * | 2014-11-21 | 2017-09-26 | 瑞泽恩制药公司 | The method and composition of targeting genetic modification is carried out using paired guide RNA |
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| CN107208078A (en) * | 2014-11-21 | 2017-09-26 | 瑞泽恩制药公司 | The method and composition of targeting genetic modification is carried out using paired guide RNA |
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| CHEN F等: ""Generation of B cell-dificient pigs by highly efficient CRISPR/Cas9-mediated gene targeting, Journal of genetics and genomics"", 《J GENET GENOMICS.》 * |
| MING ZHANG等: ""Revisiting the Pig IGHC Gene Locus in Different Breeds Uncovers Nine Distinct IGHG Genes"", 《THE JOURNAL OF IMMUNOLOGY》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114891834A (en) * | 2022-06-17 | 2022-08-12 | 吉林大学 | Method for preparing high-yield antibody rabbit based on CRISPR technology |
| CN114891834B (en) * | 2022-06-17 | 2023-10-24 | 吉林大学 | Method for preparing high-yield antibody rabbit based on CRISPR technology |
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