CN113831225A - Separation equipment and method for purifying cannabidiol monomer in cannabis extract - Google Patents
Separation equipment and method for purifying cannabidiol monomer in cannabis extract Download PDFInfo
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- CN113831225A CN113831225A CN202010600286.2A CN202010600286A CN113831225A CN 113831225 A CN113831225 A CN 113831225A CN 202010600286 A CN202010600286 A CN 202010600286A CN 113831225 A CN113831225 A CN 113831225A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/004—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring by obtaining phenols from plant material or from animal material
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
- C07C37/82—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment by solid-liquid treatment; by chemisorption
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
- C07C2601/14—The ring being saturated
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Abstract
The invention discloses separation equipment and a separation method for purifying cannabidiol monomers in cannabis extract, and the separation equipment comprises a raw material box, wherein one side of the raw material box is communicated with an ethanol solvent box, the other side of the ethanol solvent box is respectively communicated with an extraction liquid pool and an extract box, one side of the extract box, which is far away from the raw material box, is communicated with a raw material solution pool, a mobile phase pool is arranged between the raw material solution pool and the extract box, the mobile phase pool is communicated with the raw material solution pool, one side of the raw material solution pool, which is far away from the extract box, is communicated with a continuous chromatographic system, and the continuous chromatographic system comprises a first chromatographic column, a CBD material box, a second chromatographic column, a third chromatographic column, a fourth chromatographic column and a fifth chromatographic column. Has the advantages that: the super-chromatographic purification process has the advantages of high filler utilization rate, large unit filler raw material treatment capacity per unit time, high productivity and small unit product ethanol use amount.
Description
Technical Field
The invention relates to the technical field of cannabidiol extraction, in particular to a separation device and a separation method for a cannabidiol monomer purified from a cannabis extract.
Background
The most globally demanded product is currently the full spectrum of oils from which THC is removed (i.e. CBD-rich industrial hemp essential oils), and the CBD monomer. The extraction method of full spectrum oil mainly comprises the steps of flower and leaf drying or airing, crushing, leaching, chromatography, evaporative concentration and packaging. The extraction method of the CBD monomer mainly comprises the steps of flower and leaf drying or airing, crushing, leaching, chromatography, evaporative concentration, crystallization, drying, crushing homogenization and packaging. Wherein, both products need to separate CBD and THC, namely remove THC. However, because the chemical formula and molecular weight of CBD are completely consistent with THC, the physicochemical properties of CBD and THC are very close to each other, which causes a certain difficulty in separation of CBD and THC, constituting a technical barrier in the industry, and the industrial solutions are almost chromatographic purification separation.
CN106831353A discloses a method for extracting cannabidiol from cannabis sativa, which adopts a chromatographic separation mode of reverse phase resin packing of polystyrene, mainly comprising a normal phase silica gel column, an adsorption resin column and a neutral alumina column. The adsorbent resin column is a filler medium column reported to be macroporous adsorbent resin, polyamide adsorbent resin, polystyrene-based reverse phase resin and the like. Wherein, the normal phase silica gel filler has lower price and larger treatment capacity, and occupies certain application market. However, the use of normal phase silica gel has two problems, one is that the normal phase silica gel has serious dead adsorption, and some useful product components, such as CBD, are lost in the chromatographic process; secondly, the service life of the normal phase silica gel is short, and the solid waste pollution is serious. The column is frequently disassembled and assembled, so that the production efficiency is influenced, the production cost is increased, and the normal phase reagent in the waste filler volatilizes to damage the body health of staff and increase the operation risk. In addition, the resin filler has larger adsorption capacity and relatively acceptable price, can be regenerated by acid washing and alkali washing, prolongs the service life and occupies certain application market. However, not only is the dead adsorption of the resin severe, but also waste water pollution is a very important problem in regenerating the resin by acid washing or alkali washing, and evaluation of the filler is a troublesome problem in GMP and FDA certification. In addition, the price of the resin with good effect per liter is required to be thousands of yuan, even thousands of yuan. As for neutral alumina fillers, the market for applications is relatively small.
In the chromatography industry, the reversed phase silica gel has stable repeated sample loading and separation performance due to good separation performance of sample components, and GMP and FDA certification is simple, so that the reversed phase silica gel occupies more than 80% of the application amount of the application market. However, the reverse phase silica gel is generally expensive, and for high column efficiency and high efficiency separation, 10um packing is generally used under pressure, and the system pressure is high, so that the requirement on equipment becomes high, and more applications of the reverse phase silica gel are limited to a certain extent.
The traditional chromatography process mainly has the problems that:
because cannabinol components are complex and have more components similar to chemical components of CBD, after chromatography by a traditional method, the purity of CBD is not high, related impurities remain, preparation and purification are needed for two times, even three times and four times, or crystallization is carried out to sacrifice a part of yield to obtain a high-purity product. But the more times of preparation, the lower the total yield and the higher the production cost;
the high performance liquid chromatography detection of the purified product still has a small amount of THC residue, which affects the health of users and the product sale;
the chromatographic reagents are petroleum ether, normal hexane, dichloromethane and the like, the environment is polluted, the physical health of production staff is not facilitated, and the influence of the residual solvent of the product on the physical health of a user needs to be studied.
Therefore, there is an urgent need to develop a chromatographic solution with high purity of the prepared product, low toxicity of the organic reagent, environmental friendliness and low production cost, so as to meet the demand of industrial development.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
The invention provides a separation device and a separation method for purifying cannabidiol monomers in a cannabis extract, aiming at the problems in the related art, so as to overcome the technical problems in the prior related art.
Therefore, the invention adopts the following specific technical scheme:
a separation device for purifying cannabidiol monomers in cannabis extract comprises a raw material box, wherein one side of the raw material box is connected and communicated with an ethanol solvent box, the other side of the ethanol solvent box is respectively connected and communicated with an extraction liquid pool and an extract box, one side, far away from the raw material box, of the extract box is connected and communicated with a raw material solution pool, a mobile phase pool is arranged between the raw material solution pool and the extract box, the mobile phase pool is connected and communicated with the raw material solution pool, one side, far away from the extract box, of the raw material solution pool is connected and communicated with a continuous chromatographic system, the continuous chromatographic system comprises a first chromatographic column, a CBD material box, a second chromatographic column, a third chromatographic column, a fourth chromatographic column and a fifth chromatographic column, the raw material solution pool is connected and communicated with the first chromatographic column, one side, far away from the raw material solution pool, of the first chromatographic column is connected and communicated with the CBD material box, the CBD material box is far away from one side of the first chromatographic column and is connected and communicated with the second chromatographic column, the second chromatographic column is far away from one side of the first chromatographic column and is connected and communicated with the third chromatographic column, the third chromatographic column is far away from one side of the second chromatographic column and is connected and communicated with the fourth chromatographic column, the fourth chromatographic column is far away from one side of the third chromatographic column and is connected and communicated with the fifth chromatographic column, the fifth chromatographic column is connected and communicated with the raffinate pool, a front impurity pool is arranged between the raffinate pool and the fifth chromatographic column, the fifth chromatographic column is connected and communicated with the front impurity pool, the raffinate pool is far away from one side of the fifth chromatographic column and is connected and communicated with a concentration box, and the concentration box is far away from the raffinate pool and is connected and communicated with the ethanol solvent box.
Preferably, the ethanol solvent tank and the mobile phase pool are both provided with liquid inlets.
Preferably, valves are respectively arranged between the first chromatographic column and the second chromatographic column, between the second chromatographic column and the third chromatographic column, between the third chromatographic column and the fourth chromatographic column, and between the fourth chromatographic column and the fifth chromatographic column.
According to another aspect of the present invention, there is provided a method for purifying cannabidiol monomer from cannabis extract, comprising the steps of;
crushing and drying the extraction part of the hemp to obtain medicinal powder, then dissolving and carrying out hot reflux extraction by adopting ethanol water with the mass concentration of 70-100, and then carrying out reduced pressure concentration on the obtained extracting solution to obtain a concentrated fluid extract;
dissolving the concentrated fluid extract obtained in the step one in a mobile phase of 60-90% by mass of ethanol water to prepare a raw material solution;
continuously feeding the mobile phase and the obtained raw material solution in the second step into a continuous chromatographic system, feeding the mixed solution into a first chromatographic column at the speed of 8.2ml/min, internally circulating the mixed solution in the first chromatographic column, a second chromatographic column, a third chromatographic column, a fourth chromatographic column, a fifth chromatographic column and the fifth chromatographic column at the flow rate of 4.3ml/min, flowing the upper sample solution between the second chromatographic column and the third chromatographic column at the speed of 0.3ml/min, generating a high-purity CND material by the first chromatographic column, generating a precursor material by the fifth chromatographic column, continuously collecting a cannabidiol solution from a raffinate port of the continuous chromatographic system and then collecting the cannabidiol solution into an extract liquid pool, and continuously collecting a tetrahydrocannabinol solution from an extract port of the continuous chromatographic system and collecting the tetrahydrocannabinol solution into a raffinate liquid pool.
Preferably, the hot reflux extraction method in the first step is as follows: extracting with 70-100 ethanol water under reflux for 3-5 times (1.5-2.5 hr for each time), mixing extractive solutions, and concentrating under reduced pressure to obtain concentrated fluid extract.
Preferably, the mobile phase in the second step is 60-90% of ethanol water, further 70-80% of ethanol water, and further 74-76% of ethanol water.
Preferably, the continuous chromatography system described in step three has 2 to 8 chromatography columns, further 4 to 6, further 5, columns 1/1/3 respectively.
Preferably, the height of the packed column is 100-.
Preferably, the filler used in the continuous chromatography system is a reverse phase silica gel filler, and the particle size of the filler is 10-400 μm, further 15-100 μm, and further 20-40 μm.
Preferably, the temperature of the chromatographic column is 15-35 ℃, further 20-30 ℃, and the concentration of CBD in the raw material solution prepared in the second step is 40 g/L.
The invention has the beneficial effects that:
1. the process only uses an ethanol hydrosolvent, only uses one ethanol proportion for purification, only uses one ethanol proportion for regeneration, and is simple to recycle.
2. The reversed phase silica gel filler used in the process has good separation effect, simple GMP/FDA certification, reusability and environmental pollution.
3. The super-chromatographic purification process has the advantages of high filler utilization rate, large unit filler raw material treatment capacity per unit time, high productivity and small unit product ethanol use amount.
4. And (3) a super preparation and purification process, continuous sampling, continuous separation and purification are carried out, and a product with the purity of the CBD monomer of 93 percent can be obtained. And recrystallizing to obtain the CBD monomer with the purity of more than 95 percent or more than 99 percent.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a separation device for purifying cannabidiol monomers in a cannabis extract according to an embodiment of the invention;
fig. 2 is a flow chart of a method for purifying cannabidiol monomer in cannabis extract according to an embodiment of the invention.
In the figure:
1. a raw material tank; 2. an ethanol solvent tank; 3. an extraction liquid pool; 4. an extract box; 5. a mobile phase cell; 6. a raw material solution tank; 7. a first chromatographic column; 9. a second chromatography column; 10. a third chromatographic column; 11. a fourth chromatographic column; 12. a fifth chromatographic column; 13. a pre-impurity pool; 14. a raffinate tank; 15. a concentrating box.
Detailed Description
For further explanation of the various embodiments, the drawings which form a part of the disclosure and which are incorporated in and constitute a part of this specification, illustrate embodiments and, together with the description, serve to explain the principles of operation of the embodiments, and to enable others of ordinary skill in the art to understand the various embodiments and advantages of the invention, and, by reference to these figures, reference is made to the accompanying drawings, which are not to scale and wherein like reference numerals generally refer to like elements.
According to an embodiment of the invention, a separation device for purifying cannabidiol monomers in a cannabis extract is provided.
The first embodiment;
as shown in fig. 1-2, the separation apparatus for purifying cannabidiol monomer from cannabis extract according to the embodiment of the present invention includes a raw material tank 1, one side of the raw material tank 1 is connected to and communicated with an ethanol solvent tank 2, the other side of the ethanol solvent tank 2 is respectively connected to and communicated with an extraction liquid pool 3 and an extract tank 4, one side of the extract tank 4 away from the raw material tank 1 is connected to and communicated with a raw material solution pool 6, a mobile phase pool 5 is disposed between the raw material solution pool 6 and the extract tank 4, the mobile phase pool 5 is connected to and communicated with the raw material solution pool 6, one side of the raw material solution pool 6 away from the extract tank 4 is connected to and communicated with a continuous chromatography system, the continuous chromatography system includes a first chromatography column 7, a CBD material tank 8, a second chromatography column 9, a third chromatography column 10, a fourth chromatography column 11, and a fifth chromatography column 12, the raw material solution pool 6 is connected to and communicated with the first chromatography column 7, the side, far away from the raw material solution pool 6, of the first chromatographic column 7 is connected and communicated with the CBD material tank 8, the side, far away from the first chromatographic column 7, of the CBD material tank 8 is connected and communicated with the second chromatographic column 9, the side, far away from the first chromatographic column 7, of the second chromatographic column 9 is connected and communicated with the third chromatographic column 10, the side, far away from the second chromatographic column 9, of the third chromatographic column 10 is connected and communicated with the fourth chromatographic column 11, the side, far away from the third chromatographic column 10, of the fourth chromatographic column 11 is connected and communicated with the fifth chromatographic column 12, the fifth chromatographic column 12 is connected and communicated with the raffinate pool 14, a front impurity pool 13 is arranged between the raffinate pool 14 and the fifth chromatographic column 12, the fifth chromatographic column 12 is connected and communicated with the front impurity pool 13, and the raffinate pool 14, the side, far away from the fifth chromatographic column 12, is communicated with a concentration tank 15, the concentration box 15 is far away from the raffinate tank 14 and is communicated with the ethanol solvent box 2.
Example two;
as shown in fig. 1-2, liquid inlets are formed on the ethanol solvent tank 2 and the mobile phase pool 5; valves are respectively arranged between the first chromatographic column 7 and the second chromatographic column 9, between the second chromatographic column 9 and the third chromatographic column 10, between the third chromatographic column 10 and the fourth chromatographic column 11, and between the fourth chromatographic column 11 and the fifth chromatographic column 12.
Example three;
as shown in fig. 1-2, according to an embodiment of the present invention, there is also provided a method for purifying cannabidiol monomer from cannabis extract, comprising the steps of;
step S101, crushing and drying the extraction part of the cannabis sativa to obtain medicinal material powder, then dissolving the medicinal material powder by using 70-100 mass percent of ethanol water, carrying out hot reflux extraction, and then concentrating the obtained extracting solution under reduced pressure to obtain concentrated fluid extract;
step S103, dissolving the concentrated fluid extract into a mobile phase of 60-90% ethanol water by mass fraction to prepare a raw material solution;
step S105, continuously feeding the mobile phase and the obtained raw material solution into a continuous chromatographic system, feeding the mixed solution into a first chromatographic column at a speed of 8.2ml/min, internally circulating the mixed solution in the first chromatographic column, a second chromatographic column, a third chromatographic column, a fourth chromatographic column, a fifth chromatographic column and the fifth chromatographic column at a flow rate of 4.3ml/min, flowing a sample solution between the second chromatographic column and the third chromatographic column at a speed of 0.3ml/min, generating a high-purity CND material by the first chromatographic column, generating a precursor material by the fifth chromatographic column, continuously collecting a cannabidiol solution from a raffinate port of the continuous chromatographic system and then collecting the cannabidiol solution into an extract pool, continuously collecting a tetrahydrocannabinol solution from an extract port of the continuous chromatographic system and collecting the tetrahydrocannabinol solution into a raffinate pool,
example four;
as shown in fig. 1-2, the thermal reflux extraction method in the first step includes: extracting with 70-100 ethanol water under reflux for 3-5 times (1.5-2.5 hr for each time), mixing extractive solutions, and concentrating under reduced pressure to obtain concentrated fluid extract; the mobile phase in the second step is 60-90% of ethanol water, further 70-80% of ethanol water and further 74-76% of ethanol water solution.
Example five;
as shown in fig. 1-2, the continuous chromatography system described in step three has 2-8 chromatographic columns, further 4-6, further 5, columns 1/1/3 respectively; the height of the packed column bed is 100-500mm, further 100-300mm and further 200 mm; the filler used by the continuous chromatographic system is reverse phase silica gel filler, and the particle size of the filler is 10-400 μm, further 15-100 μm, and further 20-40 μm; the temperature of the chromatographic column is 15-35 ℃, further 20-30 ℃, and the concentration of CBD in the raw material solution prepared in the second step is 40 g/L.
In conclusion, by means of the technical scheme, only the ethanol water solvent is used in the process, only one ethanol proportion is used for purification, only one ethanol proportion is used for regeneration, and the recovery and the application are simple; the reversed phase silica gel filler used in the process has good separation effect, simple GMP/FDA certification, reusability and environmental pollution; the filler utilization rate of the super-chromatographic purification process is high, the unit time unit filler raw material treatment capacity is large, the productivity is high, and the unit product ethanol use amount is small; and (3) a super preparation and purification process, continuous sampling, continuous separation and purification are carried out, and a product with the purity of the CBD monomer of 93 percent can be obtained. And recrystallizing to obtain the CBD monomer with the purity of more than 95 percent or more than 99 percent.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The separation equipment for purifying cannabidiol monomers in cannabis extract is characterized by comprising a raw material box (1), one side of the raw material box (1) is connected with an ethanol solvent box (2) in a penetrating manner, the other side of the ethanol solvent box (2) is respectively connected with an extraction liquid pool (3) and an extract box (4) in a penetrating manner, one side of the extract box (4) far away from the raw material box (1) is connected with a raw material solution pool (6) in a penetrating manner, a mobile phase pool (5) is arranged between the raw material solution pool (6) and the extract box (4), the mobile phase pool (5) is connected with the raw material solution pool (6) in a penetrating manner, one side of the raw material solution pool (6) far away from the extract box (4) is connected with a continuous chromatographic system in a penetrating manner, and the continuous chromatographic system comprises a first chromatographic column (7), a CBD material box (8), a second chromatographic column (9), A third chromatographic column (10), a fourth chromatographic column (11) and a fifth chromatographic column (12), wherein the raw material solution pool (6) is connected with the first chromatographic column (7) in a run-through manner, one side of the first chromatographic column (7) far away from the raw material solution pool (6) is connected with the CBD material box (8) in a run-through manner, one side of the CBD material box (8) far away from the first chromatographic column (7) is connected with the second chromatographic column (9) in a run-through manner, one side of the second chromatographic column (9) far away from the first chromatographic column (7) is connected with the third chromatographic column (10) in a run-through manner, one side of the third chromatographic column (10) far away from the second chromatographic column (9) is connected with the fourth chromatographic column (11) in a run-through manner, one side of the fourth chromatographic column (11) far away from the third chromatographic column (10) is connected with the fifth chromatographic column (12) in a run-through manner, and the fifth chromatographic column (12) is connected with the raffinate pool (14) in a run-through manner, a front impurity pool (13) is arranged between the raffinate pool (14) and the fifth chromatographic column (12), the fifth chromatographic column (12) is connected and communicated with the front impurity pool (13), one side, far away from the fifth chromatographic column (12), of the raffinate pool (14) is connected and communicated with a concentration box (15), and the concentration box (15) is far away from the raffinate pool (14) and is connected and communicated with the ethanol solvent box (2).
2. The apparatus for separating cannabidiol monomers from cannabis extract as claimed in claim 1, wherein the ethanol solvent tank (2) and the mobile phase pool (5) are provided with liquid inlets.
3. A separation apparatus for cannabidiol monomer purification from cannabis extract as claimed in claim 1, wherein valves are provided between the first (7) and second (9) chromatographic columns, between the second (9) and third (10) chromatographic columns, between the third (10) and fourth (11) chromatographic columns, and between the fourth (11) and fifth (12) chromatographic columns respectively.
4. A method for purifying cannabidiol monomer in cannabis extract, which is used for the separation device for purifying cannabidiol monomer in cannabis extract as claimed in claim 3, comprising the following steps;
the method comprises the following steps:
crushing and drying the extraction part of the hemp to obtain medicinal powder, then dissolving and carrying out hot reflux extraction by adopting ethanol water with the mass concentration of 70-100, and then carrying out reduced pressure concentration on the obtained extracting solution to obtain a concentrated fluid extract;
step two:
dissolving the concentrated fluid extract obtained in the step one in a mobile phase of 60-90% by mass of ethanol water to prepare a raw material solution;
step three:
continuously feeding the mobile phase and the obtained raw material solution in the second step into a continuous chromatographic system, feeding the mixed solution into a first chromatographic column at the speed of 8.2ml/min, internally circulating the mixed solution in the first chromatographic column, a second chromatographic column, a third chromatographic column, a fourth chromatographic column, a fifth chromatographic column and the fifth chromatographic column at the flow rate of 4.3ml/min, flowing the upper sample solution between the second chromatographic column and the third chromatographic column at the speed of 0.3ml/min, generating a high-purity CND material by the first chromatographic column, generating a precursor material by the fifth chromatographic column, continuously collecting a diphenol solution from a raffinate port of the continuous chromatographic system and then collecting the diphenol solution into an extract pool, and continuously collecting a tetrahydrocannabinol solution from an extract port of the continuous chromatographic system and collecting the tetrahydrocannabinol solution into a raffinate pool.
5. The method for purifying cannabidiol monomer from cannabis extract as claimed in claim 4, wherein the hot reflux extraction method in step one is: extracting with 70-100 ethanol water under reflux for 3-5 times (1.5-2.5 hr for each time), mixing extractive solutions, and concentrating under reduced pressure to obtain concentrated fluid extract.
6. The method of claim 5, wherein the mobile phase in step two is 60-90% ethanol water, further 70-80% ethanol water, and further 74-76% ethanol water.
7. A method as claimed in claim 6, wherein the continuous chromatographic system in step three comprises 2-8 chromatographic columns, further 4-6 chromatographic columns, further 5 chromatographic columns 1/1/3.
8. The method as claimed in claim 7, wherein the packed column height is 100-500mm, further 100-300mm, and further 200 mm.
9. The method as claimed in claim 8, wherein the filler used in the continuous chromatography system is reverse phase silica gel filler, and the particle size of the filler is 10-400 μm, further 15-100 μm, and further 20-40 μm.
10. The method as claimed in claim 7, wherein the temperature of the chromatographic column is 15-35 deg.C, further 20-30 deg.C, and the concentration of CBD in the raw material solution prepared in the second step is 40 g/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN206244694U (en) * | 2016-10-26 | 2017-06-13 | 谢爽 | The production equipment of high-purity cannabidiol |
| CN208292897U (en) * | 2018-03-09 | 2018-12-28 | 陈天睿 | Totally-enclosed CBD automatically extracts production line |
| US20190210946A1 (en) * | 2018-01-10 | 2019-07-11 | Yantai Hemp Biotechnology Co., Ltd. | Method for preparing high-purity cannabidiol |
| CN110746275A (en) * | 2019-11-08 | 2020-02-04 | 河南汉麻生物科技有限公司 | Method for separating cannabidiol by using continuous chromatographic system |
-
2020
- 2020-06-24 CN CN202010600286.2A patent/CN113831225A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206244694U (en) * | 2016-10-26 | 2017-06-13 | 谢爽 | The production equipment of high-purity cannabidiol |
| US20190210946A1 (en) * | 2018-01-10 | 2019-07-11 | Yantai Hemp Biotechnology Co., Ltd. | Method for preparing high-purity cannabidiol |
| CN208292897U (en) * | 2018-03-09 | 2018-12-28 | 陈天睿 | Totally-enclosed CBD automatically extracts production line |
| CN110746275A (en) * | 2019-11-08 | 2020-02-04 | 河南汉麻生物科技有限公司 | Method for separating cannabidiol by using continuous chromatographic system |
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