CN113827617B - Application of airway basal layer stem cells in benign airway stenosis treatment - Google Patents
Application of airway basal layer stem cells in benign airway stenosis treatment Download PDFInfo
- Publication number
- CN113827617B CN113827617B CN202110713339.6A CN202110713339A CN113827617B CN 113827617 B CN113827617 B CN 113827617B CN 202110713339 A CN202110713339 A CN 202110713339A CN 113827617 B CN113827617 B CN 113827617B
- Authority
- CN
- China
- Prior art keywords
- airway
- basal layer
- stem cells
- stenosis
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/42—Respiratory system, e.g. lungs, bronchi or lung cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3882—Hollow organs, e.g. bladder, esophagus, urether, uterus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/22—Materials or treatment for tissue regeneration for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Botany (AREA)
- Urology & Nephrology (AREA)
- Developmental Biology & Embryology (AREA)
- Pulmonology (AREA)
- Transplantation (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Physiology (AREA)
- Hematology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Reproductive Health (AREA)
- Vascular Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to the use of airway-basal stem cells in the treatment of benign airway stenosis. The research of the invention shows that the airway basal layer stem cells can be used for reconstructing normal airway epithelium on the surface of the airway stenosis section and inhibiting the proliferation of the granulation tissue of the stenosis section, so that the treatment method of removing the granulation tissue of the stenosis section and then transplanting the airway basal layer stem cells is adopted, the tracheal stenosis can be treated obviously, the stenosis recurrence after conventional treatment is effectively avoided, the tracheal stenosis tends to be stable, and the quality of life of a patient is greatly improved.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to application of airway basal layer stem cells in benign airway stenosis treatment.
Background
Benign airway stenosis is a common disease in interventional respiratory pathology, is mostly caused by tuberculosis or tracheal intubation, takes obvious granulation tissue hyperplasia as a main pathological change, seriously influences the respiratory function of a patient, and can cause asphyxia. The current mainstream treatment method is surgical treatment or bronchoscopic intervention treatment.
The parallel end-to-end anastomosis of the excision of the narrow section through an operation is a conventional treatment means for benign airway stenosis, has remarkable curative effect, can completely recover the smoothness of a tracheal lumen, has higher requirement on the general condition of the body of a patient in the operation treatment, has limitation on the length of the narrow section, can not be excised through the operation because the tension of an anastomosis port is too high when the stenosis exceeds 5cm, and only a small part of patients can tolerate the operation treatment. On the other hand, although the surgical treatment has few complications, the granulation tissue proliferation still appears on part of patients at the anastomotic site, which results in the occurrence of restenosis.
Bronchoscope interventional therapy mainly clears away narrow section granulation tissue through modes such as sacculus expansion, high frequency electrotome, laser ablation in order to reach the unobstructed effect of recovery lumen, is main treatment means to the benign narrow patient of air flue who can't accept the operation. The intervention treatment has obvious short-term curative effect, but cannot inhibit the proliferation of granulation tissues, the restenosis occurs within weeks or months, and patients often need to receive bronchoscopic intervention regularly to maintain the diameter of a narrow segment of a trachea.
Generally, both surgical treatment and bronchoscopic interventional treatment are limited in many ways, and do not effectively treat benign airway stenosis.
The airway epithelium has the function of inhibiting proliferation and differentiation of fibroblasts while protecting the submucosal tissue from external stimulation. The airway basal layer cells are taken as stem cells which can be differentiated into various airway epithelial cells, and receive wide attention from tissue engineering, and researchers inoculate autologous chondrocytes and autologous respiratory epithelial cells obtained by inducing differentiation of bone marrow mesenchymal stem cells into a decellularized scaffold in a bioreactor, and transplant the decellularized scaffold into a patient body to replace a left main bronchus softened by the patient. According to the report of the literature (DOI: 10.29235/1814-6023-2020-17-4-417-426), the mesenchymal stem cells from the autologous mucosa are adopted to treat laryngotracheal stenosis, the respiratory function is improved, the exercise tolerance is improved, and the short-term curative effect is obvious. But in the long term, mesenchymal stem cells are mainly relied on for their anti-inflammatory and growth-promoting effects, since they cannot form epithelial tissues. Therefore, the benign airway stenosis cannot be radically treated by using the mesenchymal stem cells, and the disease is always relapsed.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a long-acting stem cell therapy for benign airway stenosis. The treatment method firstly removes granulation tissues in the narrow section, and then transplants the airway basal layer stem cells, so that normal airway epithelium can be reconstructed on the surface of the narrow section of the airway, the proliferation of the granulation tissues in the narrow section is inhibited, the recurrence of stenosis after conventional treatment is effectively avoided, the tracheal stenosis tends to be stable, and the quality of life of patients is greatly improved.
The invention aims to provide application of airway basal layer stem cells in treatment of benign airway stenosis.
The invention also aims to provide application of the airway basal layer stem cells in preparing stem cell medicines for treating benign airway stenosis.
It is another object of the present invention to provide a suspension of airway basal layer stem cells for the treatment of benign airway stenosis.
In order to achieve the purpose, the invention provides the following technical scheme:
no researchers have proposed a viable approach to the treatment of benign airway stenosis through regenerative medicine, and the feasibility and importance of reconstructing epithelial tissue to treat airway stenosis has not been appreciated by the medical community. However, through the research of the team, the airway basal layer stem cells are determined to be capable of effectively treating benign airway stenosis, and the scheme for treating the benign airway stenosis by adopting the airway basal layer stem cells is determined for the first time.
The invention therefore claims:
use of airway basal layer stem cells in the treatment of benign airway stenosis.
Application of airway basal layer stem cells in preparing stem cell medicines for treating benign airway stenosis.
Preferably, the airway basal layer stem cells are autologous.
More preferably, the airway basal layer stem cells are taken from the airway narrowing grade 2-3 bronchial epithelium.
An airway basal layer stem cell suspension for treating benign airway stenosis, wherein the cell density of the stem cell suspension is 400-600 ten thousand per mL.
Wherein, preferably, the cell density of the stem cell suspension is 500 ten thousand/mL.
Wherein, preferably, the dispersion medium of the stem cell suspension is physiological saline or phosphate buffer.
Wherein, preferably, the pH value of the phosphate buffer solution is 6-8.
Preferably, the stem cell suspension contains 1-5% (w/v) of human serum albumin, and more preferably 3% (w/v) of human serum albumin.
The airway basal layer stem cell suspension for treating benign airway stenosis can be applied to preparation of stem cell medicines for treating benign airway stenosis.
The invention has the following beneficial effects:
the invention provides a feasible method for treating benign airway stenosis through regenerative medicine, which combines the removal of granulation tissue in a narrow section with airway basal layer stem cell transplantation for treatment:
(1) Provides an applicable stem cell for stem cell treatment of airway stenosis.
(2) Can rebuild normal airway epithelium on the surface of airway stenosis segment.
(3) Can effectively reduce the restenosis of benign airway stenosis and stabilize airway granulation.
(4) Improving the life quality of the patients.
Drawings
Figure 1 shows the morphology of airway basal layer stem cells (light mirror).
FIG. 2 shows the immunofluorescence of airway basal layer stem cells to identify KRT5/P63 expression.
Fig. 3 shows the trachea after model canines from the placebo group were modeled.
Fig. 4 shows the treatment results of the first model dog in the experimental group for treating tracheal injury by autologous airway basal layer stem cells.
Fig. 5 shows the treatment results of the second model dog in the experimental group for the treatment of tracheal injury by autologous airway basal layer stem cells.
Fig. 6 shows the treatment results of the third model dog in the experimental group for the treatment of tracheal injury by autologous airway basal layer stem cells.
Fig. 7 shows the treatment results of the BMSC control group model dog for tracheal injury treatment via mesenchymal stem cells of autologous bone marrow.
Detailed Description
The invention is further described with reference to the drawings and the following detailed description, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated. Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 airway stenosis model establishment
Randomly selecting 9 healthy adult beagle dogs, establishing a benign airway stenosis model after intubation in a mode of pressing the beagle dog airway for 24 hours by a tracheal intubation saccule, and successfully modeling after examination and verification.
Successfully constructed models were randomly divided into 3 groups (experimental, blank and BMSC control), with 3 of each group.
Example 2 extraction, isolation, identification and culture of airway basal layer Stem cells
Using a cell brush to brush 2-3 grade bronchial epithelium of the stricture of the airway of a beagle dog (experimental beagle dog) under the guidance of a bronchoscope in advance for 2 weeks to obtain autologous airway basal layer stem cells;
the primary Cells obtained by brushing were expanded according to the culture method described in the literature (Rapid Expansion of Human epidermal Stem Cells capable of maintaining the expression level of krt5/p63 Cells, and the type of Cells cultured was identified by immunofluorescence techniques.
As a result: the cell morphology obtained by the culture is shown in figure 1, the immunofluorescence technology shows that the krt5/p63 expression is positive as shown in figure 2, and the cultured cells are airway basal layer stem cells.
EXAMPLE 3 treatment of airway stenosis with airway basal layer Stem cells
A great amount of necrotic substances at the injured part can be seen 3 days after the beagle is modeled, severe tracheal stenosis appears 7 days later, and the beagle is treated by placing a metal bracket conventionally.
(1) Removing granulation tissue of the model dog in the narrow section by a conventional treatment method 3 days before cell transplantation, and measuring the length of the narrow section of the model dog;
(2) Preparing stem cells:
experimental groups: on the day of cell transplantation, according to the proportion of transplanting 500 ten thousand cells per 1 centimeter of the length of a narrow section, digesting the required airway basal layer stem cells, using PBS to resuspend the cells into a cell suspension with the concentration of about 500 ten thousand/mL, and adding human serum albumin until the mass concentration is 3%;
BMSC control group: on the day of cell transplantation, according to the proportion of 500 ten thousand cells transplanted per 1 cm of the length of a narrow section, required umbilical cord mesenchymal stem cells (derived from a BMSC control group beagle dog) are digested and resuspended into a cell suspension with the concentration of about 500 ten thousand/mL by using PBS, and human albumin is added to the mass concentration of 3%;
(3) Before cell transplantation, the endocrine in the airway is completely sucked through a bronchoscope;
(4) Cutting off a nozzle at the end of the spray tube head, and sending the spray tube into the trachea through a bronchoscope;
(5) Cell transplantation:
experimental group model dogs: sucking the stem cell suspension of the autologous airway basal layer obtained in the example 2 by using a syringe, slowly pushing the stem cell suspension through a spraying tube, and uniformly coating the stem cell suspension on the surface of a narrow section;
BMSC control group model dogs: sucking the umbilical cord mesenchymal stem cell suspension by using an injector, slowly pushing the umbilical cord mesenchymal stem cell suspension through a spray pipe, and uniformly smearing the umbilical cord mesenchymal stem cell suspension on the surface of a narrow section;
blank control group model dogs: sucking equivalent PBS containing 3% human serum albumin by using an injector, slowly pushing the PBS through a spray tube, and uniformly coating the PBS on the surface of a narrow section;
(6) Repeating the steps (1) to (5) two to three times at intervals of 1 week.
As a result: as shown in fig. 3, the control group model dog is expressed after modeling, after the metal stent is placed for treatment, the trachea is recovered to be unobstructed, the diameter of the trachea can be maintained in a short period, but the trachea is restenosis after about one week, and after granulation tissues are removed, the treatment by smearing physiological saline containing 3% human albumin is ineffective, so that lethal tracheal stenosis is formed.
As shown in figures 4-6, the treatment results of 3 model dogs in the experimental group for treating tracheal injury by autologous airway basal layer stem cells are shown, and the metal stent is placed for treatment to restore unobstructed trachea, but tracheal restenosis occurs after one week. After granulation tissues are removed, airway basal layer stem cells are transplanted, smooth airway epithelium can be seen on the surface of the narrow section after two times of transplantation, and no obvious progress can be made in the narrow section within 1 month.
And because tracheal stenosis that did not recur for 3 months tended to be clinically cured, our animal experiments were also followed for 3 months, and the results showed that the stenotic tissue remained stable for 3 months after successful cell transplantation.
The treatment of the invention thus allows complete stabilization of the stenosed part with the placement of a metal stent and ensures that it does not recur for at least 3 months, with a long period of stabilization being expected.
Meanwhile, a contrast experiment is carried out by replacing airway basal layer stem cells with bone marrow mesenchymal stem cells (BMSC), and as shown in figure 7, no obvious progress is observed in the observed stenosis after one week of umbilical cord mesenchymal stem cell transplantation treatment, but restenosis recurs after two weeks of cell transplantation, and the lumen is severely narrowed. From the results of the experiment, the mesenchymal stem cells can stabilize the narrow tissue only within one to two weeks.
Further specifically stated: the main reason why the metal stent is placed before stem cell treatment is that the current tracheal stenosis modeling means can cause tracheal cartilage destruction in different degrees, and the lumen can be narrowed along with respiration, so that the metal stent needs to be placed for support. On the other hand, however, the metal stent itself promotes the growth of granulation tissue, resulting in the most clinically intractable tracheal stenosis. Therefore, the results of the invention show that under the condition of placing a metal bracket which is a large interference factor, the airway basal layer stem cell transplantation therapy of the invention can still effectively stabilize the narrow tissue, and the curative effect is proved to be excellent.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. Application of airway basal layer stem cells in preparing a medicament for treating benign airway stenosis.
2. The use of claim 1, wherein the airway basal layer stem cells are autologous.
3. The use according to claim 1 or 2, wherein the airway basal layer stem cells are taken from the bronchial epithelium of level 2-3 airway stenosis.
4. Use of a suspension of airway basal layer stem cells in the manufacture of a medicament for the treatment of stem cells associated with benign airway stenosis.
5. The use of claim 4, wherein the airway basal layer stem cell suspension has a cell density of 400 to 600 ten thousand per mL.
6. The use of claim 5, wherein the cell density of the airway basal layer stem cell suspension is 500 ten thousand per mL.
7. The use according to claim 4, wherein the dispersion medium of the airway basal layer stem cell suspension is physiological saline or phosphate buffer.
8. The use according to claim 7, wherein the phosphate buffer has a pH of 6 to 8.
9. The use according to any one of claims 4 to 8, wherein the airway basal layer stem cell suspension contains 1% to 5% (w/v) human serum albumin.
10. The use of claim 9, wherein the airway basal layer stem cell suspension comprises 3% (w/v) human serum albumin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110713339.6A CN113827617B (en) | 2021-06-25 | 2021-06-25 | Application of airway basal layer stem cells in benign airway stenosis treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110713339.6A CN113827617B (en) | 2021-06-25 | 2021-06-25 | Application of airway basal layer stem cells in benign airway stenosis treatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113827617A CN113827617A (en) | 2021-12-24 |
| CN113827617B true CN113827617B (en) | 2022-11-18 |
Family
ID=78962777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110713339.6A Active CN113827617B (en) | 2021-06-25 | 2021-06-25 | Application of airway basal layer stem cells in benign airway stenosis treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113827617B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114196613B (en) * | 2019-05-16 | 2024-03-12 | 苏州吉美瑞生医学科技有限公司 | Application of bronchial basal layer cells in preparation of medicaments for treating bronchiectasis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109689071A (en) * | 2016-05-16 | 2019-04-26 | 通用医疗公司 | Popularity road stem cell in lung epithelial engineering |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066802A2 (en) * | 2011-10-27 | 2013-05-10 | Agency For Science, Technology And Research (A*Star) | Compositions and methods for lung regeneration |
| US10624992B2 (en) * | 2016-05-16 | 2020-04-21 | The General Hospital Corporation | Human airway stem cells in lung epithelial engineering |
| CN107974429B (en) * | 2017-11-29 | 2021-06-29 | 宁夏医科大学总医院 | Method for rapidly separating and culturing human airway epithelial cells and optimized culture medium |
| WO2020132248A1 (en) * | 2018-12-21 | 2020-06-25 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for airway tissue regeneration |
-
2021
- 2021-06-25 CN CN202110713339.6A patent/CN113827617B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109689071A (en) * | 2016-05-16 | 2019-04-26 | 通用医疗公司 | Popularity road stem cell in lung epithelial engineering |
Non-Patent Citations (3)
| Title |
|---|
| "Increased expression of TROP2 in airway basal cells potentially contributes to airway remodeling in chronic obstructive pulmonary disease";Qixiao Liu et al.;《Respiratory Research》;20161125;第17卷(第159期);第1-14页 * |
| "几种影响小鼠气管上皮基底干细胞体外增及分化潜能因素的研究";高静韬等;《国际呼吸杂志》;20170831;第37卷(第15期);第1123页第1.2.1节 * |
| "肺上皮干/祖细胞种类和研究方法进展";邓敏华等;《中国肺癌杂志》;20170228;第20卷(第2期);第130-137页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113827617A (en) | 2021-12-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rehmani et al. | Three-dimensional-printed bioengineered tracheal grafts: preclinical results and potential for human use | |
| Kalathur et al. | Translating tissue-engineered tracheal replacement from bench to bedside | |
| EP1950284B1 (en) | Method for cultivation of hair follicular dermal sheath cell | |
| Walles | Tracheobronchial bio-engineering: biotechnology fulfilling unmet medical needs | |
| US20140058508A1 (en) | Synthetic scaffolds and organ and tissue transplantation | |
| JP2002506082A5 (en) | ||
| CN113827617B (en) | Application of airway basal layer stem cells in benign airway stenosis treatment | |
| US20100028308A1 (en) | Methods to maintain, improve and restore the cartilage phenotype of chondrocytes | |
| KR20160016778A (en) | Method and composition for treating inflammatory bowel disease without colectomy | |
| Nakamura et al. | Tissue-engineered airway and “in situ tissue engineering” | |
| Hashemibeni et al. | An animal model study for repair of tracheal defects with autologous stem cells and differentiated chondrocytes from adipose-derived stem cells | |
| JP6958846B1 (en) | Method for producing synovial membrane-derived mesenchymal stem cells and method for producing cell preparation for joint treatment | |
| Seguin et al. | Carinal replacement with an aortic allograft | |
| Al-Ayoubi et al. | Reconstruction of anterior tracheal defects using a bioengineered graft in a porcine model | |
| Schuller et al. | Reconstruction of the larynx and trachea | |
| CN111566204B (en) | Method for producing cultured cells and method for producing therapeutic agent for spinal cord injury disease | |
| CN101721262A (en) | Tissue engineering combined human body lumen succedaneum | |
| JP6929346B2 (en) | Cartilage differentiation promoting complex containing achondroplasia cells and stem cells and their uses | |
| Llames et al. | Tissue bioengineering and artificial organs | |
| WO2008146997A1 (en) | Implants for reconstructing mucosal lumen | |
| KR20200097245A (en) | Reduction of elastin allowing recellularization of cartilage implants | |
| RU2731314C1 (en) | Method for production of spheroids for cartilage repair | |
| Okada et al. | Surgical treatment of laryngotracheal stenosis by a trough technique | |
| Melgarejo-Ramírez et al. | Novel Therapy for Acquired Tracheomalacia with a Tissue-Engineered Extraluminal Tracheal Splint and Autologous Mesenchymal-Derived Chondrocytes | |
| Jacobson et al. | Tracheal replacement revisited: use of a vascularized tracheal transplant in a porcine model |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |