CN113817591A - Portable integrated biological experiment device - Google Patents

Portable integrated biological experiment device Download PDF

Info

Publication number
CN113817591A
CN113817591A CN202010556895.2A CN202010556895A CN113817591A CN 113817591 A CN113817591 A CN 113817591A CN 202010556895 A CN202010556895 A CN 202010556895A CN 113817591 A CN113817591 A CN 113817591A
Authority
CN
China
Prior art keywords
module
microscope
functional
portable integrated
centrifugal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010556895.2A
Other languages
Chinese (zh)
Inventor
王�忠
杜晓英
万玉明
崔金明
周宇
李小明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Institute of Advanced Technology of CAS
Original Assignee
Guangzhou Institute of Advanced Technology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Institute of Advanced Technology of CAS filed Critical Guangzhou Institute of Advanced Technology of CAS
Priority to CN202010556895.2A priority Critical patent/CN113817591A/en
Priority to PCT/CN2020/097780 priority patent/WO2021253471A1/en
Publication of CN113817591A publication Critical patent/CN113817591A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00Enclosures; Chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00Supporting devices; Holding devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/02Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/34Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38Temperature-responsive control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Thermal Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a portable integrated biological experiment device, which comprises an equipment shell, functional modules and a control center, wherein the equipment shell is provided with a plurality of functional modules; each functional module is integrated into the equipment shell; the control center is connected with each functional module and is used for controlling each functional module respectively. The invention realizes that various experimental steps can be completely finished on one instrument. Adopt miniaturization, modularized design, make the instrument portable, can put at home or even the level of field operation. Therefore, more biological interest enthusiasts outside the laboratory are given the opportunity to experience the interest of the biological experiment in person. On the other hand, the research base number can be greatly expanded by guiding wider personnel to carry out simpler biological experiments in daily life and carrying out preliminary screening on the microorganisms, and biological researchers carry out deeper research on the basis to discover more microorganisms with application prospects for social use.

Description

Portable integrated biological experiment device
Technical Field
The invention relates to the technical field of biological experiment equipment, in particular to a portable integrated biological experiment device.
Background
At present, various experimental instruments (such as a shaking table, a centrifuge, a temperature control device, an electric rotating instrument, an electrophoresis instrument, a microscope and the like) in a biological laboratory realize single functions, have large volume, are respectively and independently fixedly arranged at different positions in the laboratory, the price of various experimental equipment is relatively high, and a scientific researcher can only finish a biological experiment by using various equipment in the laboratory. This situation greatly limits the possibility of biological interest enthusiasts outside the biological laboratory to participate in biological experiments. In order to enable more biological interest enthusiasts outside laboratories to have the opportunity to experience the interest of biological experiments in person, the invention aims to develop an integrated biological experiment device, and various experimental steps (such as shaking table culture, centrifugation, heating, cooling, electric conversion, electrophoresis, microscope observation and the like) can be completely finished on one instrument. Adopt miniaturization, modularized design, make the instrument portable, can put at home or even the level of field operation. On the other hand, microorganisms are ubiquitous and various in nature, the types of microorganisms which are mastered and utilized by human beings at present are only a small part, the research base number can be greatly expanded by guiding wider personnel to carry out simpler biological experiments in daily life and carrying out primary screening on the microorganisms, and biological researchers carry out deeper research on the basis to discover more microorganisms with application prospects and use the microorganisms for society.
CN205347432U discloses a modular microorganism experimental apparatus, which comprises a plurality of functional modules, a plurality of mounting substrates, an apparatus cabinet and a control center, wherein the apparatus adopts a modular design to integrate various functional modules into an apparatus cabinet with a multi-layer structure, and realizes automatic control. It is clear from this patent specification that the device is obviously not portable, is still an instrument that can only be present in the laboratory, and that the technical complexity is high and the cost is high.
Disclosure of Invention
In view of the above, in order to solve the above problems in the prior art, the present invention provides a portable integrated biological experiment apparatus, which can complete various experimental steps (such as shake culture, centrifugation, electrical rotation, heating, cooling, electrophoresis, microscopic observation, etc.) in one instrument.
The invention solves the problems through the following technical means:
a portable integrated biological assay device, comprising:
an equipment housing;
each functional module is integrated into the equipment shell;
and the control center is connected with each functional module and is used for controlling each functional module respectively.
Further, each functional module comprises a shake culture module, and the shake culture module comprises:
a shaking table module box body;
the eccentric turntable is arranged in the shaking table module box body and is used for fixing the microorganisms to be cultured and realizing the shaking table culture function;
the first driving motor is used for driving the eccentric turntable to rotate so as to realize back-and-forth shaking;
the electric heating block is arranged in the shaking table module box body;
the fan is arranged on the shaking table module box body;
temperature and humidity sensor sets up in shaking table module box, and electrical heating piece, fan and temperature and humidity sensor are used for maintaining suitable cultivation temperature and humidity in the box.
Further, each of the functional modules includes a centrifuge module, the centrifuge module including:
a centrifugal chamber;
the centrifugal rotor is arranged in the centrifugal cavity and is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
a motor fixing seat;
and the second driving motor is fixed on the motor fixing seat and used for driving the centrifugal rotor to rotate at a high speed to realize a centrifugal function.
Further, the overall outline dimension of the centrifugal module is as follows: length, width, height, 100, 90 (mm).
Further, each of the functional modules includes a heating module including:
the first heat-conducting block is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
the heating sheet is used for heating the first heat conduction block;
the first temperature sensor is arranged in the first heat conduction block and used for feeding back a temperature signal to control, so that the first heat conduction block maintains the set temperature.
Further, each of the functional modules includes a refrigeration module, and the refrigeration module includes:
the second heat-conducting block is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
the cold end of the semiconductor refrigeration sheet is connected with the second heat conduction block and is used for refrigerating the second heat conduction block;
the second temperature sensor is arranged in the second heat conduction block and used for feeding back a temperature signal to control, so that the second heat conduction block maintains the set temperature;
the radiating fin is arranged below the semiconductor refrigerating fin;
and the heat radiation fan is arranged below the heat radiation fin.
Further, each of the functional modules includes a microscope module, and the microscope module includes:
a microscope housing having a microscope optical amplification section integrated therein;
an objective lens window disposed on the microscope housing;
the illumination light source is arranged on the microscope shell and is opposite to the objective window;
and the focusing knob is arranged on the microscope shell, is adjusted during observation, and displays the amplified image on the control center.
Further, the overall outline dimensions of the microscope module are as follows: length, width, height, 60, 140 (mm).
Further, the control center is arranged on the equipment shell or on the mobile phone of the user.
Furthermore, each functional module is a module of various instruments in a laboratory after miniaturization and modular design, or a module for realizing various new requirements, and the functional modules are increased or decreased according to actual needs; the functional modules developed aiming at the same functional instrument have different specific implementation modes.
Compared with the prior art, the invention has the beneficial effects that at least:
1. the existing various laboratory instruments are integrated into one instrument, and the instrument realizes various experimental steps required by a biological experiment;
2. the device adopts a miniaturized and modularized design, is convenient to carry, has relatively low cost, and ensures that biological experiments are not limited to be carried out in a laboratory;
3. biological experiments are not limited to be carried out in a laboratory, people outside the vast laboratory have the opportunity to participate in the work of microorganism excavation, strain screening and the like, and the method is suitable for popularization to common individual users.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic view of the structure of a biological experimental apparatus according to the present invention;
FIG. 2 is a schematic diagram of the structure of a shake culture module according to the present invention;
FIG. 3 is a schematic structural view of a centrifuge module of the present invention;
FIG. 4 is a schematic view of the heating module of the present invention;
FIG. 5 is a schematic diagram of the construction of the refrigeration module of the present invention;
FIG. 6 is a schematic structural view of a microscope module according to the present invention;
FIG. 7 is a schematic structural diagram of an exemplary functional module integrated with the biological experimental apparatus according to the present invention.
Description of reference numerals:
1. an equipment housing; 2. each functional module; 3. a control center; 21. a shaking table culture module; 22. a centrifuge module; 23. a microscope module; 24. a refrigeration module; 25. a heating module; 201. a shaking table module box body; 202. an eccentric turntable; 203. a first drive motor; 204. an electrical heating block; 205. a fan; 206. a temperature and humidity sensor; 201', a centrifugal cavity; 202', a centrifugal rotor; 203', a second driving motor; 204', a motor fixing seat; 201', a first heat conducting block; 202', heating plate; 203' ″, a first temperature sensor; 201', a second heat conducting block; 202', a second temperature sensor; 203', semiconductor chilling plates; 204', cooling fins; 205', radiator fan; 201', microscope housing; 202 "', objective window; 203', a lighting source; 204', focusing knob.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work based on the embodiments of the present invention belong to the protection scope of the present invention.
Examples
As shown in fig. 1, the portable integrated biological experiment apparatus of the present invention mainly comprises: the equipment comprises an equipment shell 1, various functional modules 2 and a control center 3. The miniature and modular design is adopted, existing laboratory instruments (such as a shaking table, a centrifugal machine, temperature control equipment, an electric rotating instrument, an electrophoresis apparatus, a microscope and the like) in various laboratories are designed into corresponding functional modules, all the functional modules 2 are integrated into an equipment shell 1, and all the functional modules 2 are respectively controlled through a control center 3.
Each functional module 2 can be a module of various instruments in a laboratory after miniaturization and modular design, and can also be a module for realizing various new requirements, and the functional modules can be increased or decreased according to actual needs. The functional modules developed for the same functional instrument can also have different specific implementation modes.
The following examples of miniaturized, modular design are made for some of the more commonly used functional modules:
1. shaking table culture module
As shown in FIG. 2, the shake culture module 21 comprises a shake module housing 201, an eccentric turntable 202, a first driving motor 203, an electric heating block 204, a fan 205 and a temperature/humidity sensor 206.
The eccentric turntable 202 is arranged in the shaking table module box 201 and is used for fixing microorganisms to be cultured so as to realize the shaking table culture function; the first driving motor 203 is used for driving the eccentric turntable 202 to rotate so as to realize back-and-forth shaking; the electric heating block 204 is arranged in the shaking table module box 201; the fan 205 is arranged on the shaking table module box 201; the temperature and humidity sensor 206 is disposed in the shaking table module box 201, and the electric heating block 204, the fan 205 and the temperature and humidity sensor 206 are used to maintain the proper cultivation temperature and humidity in the box.
When in use, the microorganism to be cultured is fixed on the eccentric turntable 202, thereby realizing the function of shaking table culture. The overall contour dimension of the shake culture module 21 is as follows: length, width, height, 100, 75 (mm).
2. Centrifugal module
As shown in fig. 3, the centrifugal module 22 includes a centrifugal chamber 201 ', a centrifugal rotor 202', a second driving motor 203 ', and a motor holder 204'.
The centrifugal rotor 202 ' is arranged in the centrifugal cavity 201 ', and a hole for placing a centrifugal tube filled with bacteria liquid is formed in the centrifugal rotor 202 '; the second driving motor 203 ' is fixed on the motor fixing seat 204 ' and is used for driving the centrifugal rotor 202 ' to rotate at a high speed, so as to realize the centrifugal function.
The centrifugal tube filled with the bacteria liquid is placed in the hole of the centrifugal rotor 202 ', and the second driving motor 203 ' is started to drive the centrifugal rotor 202 ' to rotate at a high speed, so that the centrifugal function is realized. The overall profile dimensions of the centrifugal module 22 are: length, width, height, 100, 90 (mm).
3. Heating module
As shown in fig. 4, the heating module 25 includes a first heat conduction block 201 ', a heating fin 202 ', and a first temperature sensor 203 '.
The first heat conduction block 201' is provided with a hole for placing a centrifugal tube filled with bacteria liquid; a heating fin 202 'for heating the first heat conduction block 201'; a first temperature sensor 203 ' is provided in the first heat conduction block 201 ' for feeding back a temperature signal to control so that the first heat conduction block 201 ' maintains a set temperature.
The heating of the bacteria liquid can be realized by putting the centrifuge tube filled with the bacteria liquid into the hole of the first heat-conducting block 201'. The overall external contour dimensions of the heating module 25 are: length, width, height, 60, 20 (mm).
4. Refrigeration module
As shown in fig. 5, the cooling module 24 comprises a second heat conducting block 201 ', a second temperature sensor 202 ', a semiconductor cooling plate 203 ', a heat sink 204 ' and a heat dissipating fan 205 '.
The second heat conduction block 201' is provided with a hole for placing a centrifugal tube filled with bacteria liquid; the cold end of the semiconductor refrigerating sheet 203 ' is connected with the second heat conducting block 201 ' and is used for refrigerating the second heat conducting block 201 '; a second temperature sensor 202 ' is provided in the second heat conduction block 201 ' for feeding back a temperature signal to control so as to maintain the second heat conduction block 201 ' at a set temperature; the cooling fin 204 'is provided under the semiconductor chilling plate 203'; the radiator fan 205 "'is arranged below the heat sink 204"'.
The temperature of the bacteria liquid can be reduced by putting the centrifuge tube filled with the bacteria liquid into the hole of the second heat-conducting block 201'. The overall profile dimensions of the refrigeration module 24 are: length, width, height, 100, 80 (mm).
5. Microscope module
As shown in fig. 6, the microscope module 23 comprises a microscope housing 201 "', an objective lens window 202"', an illumination light source 203 "', and a focusing knob 204"'.
The microscope optical enlargement is located in a microscope housing 201 ' ″, with an objective window 202 ' ″ arranged on the microscope housing 201 ' ″; the illumination source 203 "' is arranged on the microscope housing 201" ', directly opposite the objective window 202 "'; the focusing knob 204 "'is provided on the microscope housing 201"'.
The slide holding the germs to be observed is placed in alignment with the objective lens window 202 ', the illumination light source 203 "' is turned on, the focusing knob 204" ' is adjusted, and the image enlarged by the microscope can be displayed on the display screen of the control center 3 or on a connected cell phone. The overall outline dimensions of the microscope module 23 are: length, width, height, 60, 140
(mm)。
Fig. 7 is a schematic diagram of an overall structure of an apparatus integrating the above exemplary functional modules, where the apparatus includes the functional modules: a shaking table culture module 21, a centrifugation module 22, a microscope module 23, a refrigeration module 24 and a heating module 25; the overall outline dimensions of the device in the figure are: length, width and height 360, 240, 110(mm), the height of the microscope module 23 part is 150mm, the whole weight of the equipment is about 5kg, and the equipment can be conveniently moved and carried.
The biological experimental device can completely complete various experimental steps (such as shaking culture, centrifugation, electric rotation, heating, cooling, electrophoresis, microscope observation and the like) on one instrument. Adopt miniaturization, modularized design, make the instrument portable, can put at home or even the level of field operation. Therefore, more biological interest enthusiasts outside the laboratory are given the opportunity to experience the interest of the biological experiment in person. On the other hand, microorganisms are ubiquitous and various in nature, the types of microorganisms which are mastered and utilized by human beings at present are only a small part, the research base number can be greatly expanded by guiding wider personnel to carry out simpler biological experiments in daily life and carrying out primary screening on the microorganisms, and biological researchers carry out deeper research on the basis to discover more microorganisms with application prospects and use the microorganisms for society.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A portable integrated biological experiment device, which is characterized by comprising:
an equipment housing;
each functional module is integrated into the equipment shell;
and the control center is connected with each functional module and is used for controlling each functional module respectively.
2. The portable integrated biological testing device of claim 1, wherein each of said functional modules comprises a shake culture module, said shake culture module comprising:
a shaking table module box body;
the eccentric turntable is arranged in the shaking table module box body and is used for fixing the microorganisms to be cultured and realizing the shaking table culture function;
the first driving motor is used for driving the eccentric turntable to rotate so as to realize back-and-forth shaking;
the electric heating block is arranged in the shaking table module box body;
the fan is arranged on the shaking table module box body;
temperature and humidity sensor sets up in shaking table module box, and electrical heating piece, fan and temperature and humidity sensor are used for maintaining suitable cultivation temperature and humidity in the box.
3. The portable integrated biological assay device of claim 1, wherein each functional module comprises a centrifuge module, the centrifuge module comprising:
a centrifugal chamber;
the centrifugal rotor is arranged in the centrifugal cavity and is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
a motor fixing seat;
and the second driving motor is fixed on the motor fixing seat and used for driving the centrifugal rotor to rotate at a high speed to realize a centrifugal function.
4. A portable integrated biological assay device, as claimed in claim 3, wherein the centrifuge module has overall physical dimensions of: length, width, height, 100, 90 (mm).
5. The portable integrated biological assay device of claim 1, wherein each functional module comprises a heating module comprising:
the first heat-conducting block is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
the heating sheet is used for heating the first heat conduction block;
the first temperature sensor is arranged in the first heat conduction block and used for feeding back a temperature signal to control, so that the first heat conduction block maintains the set temperature.
6. The portable integrated biological assay device of claim 1, wherein each functional module comprises a refrigeration module, the refrigeration module comprising:
the second heat-conducting block is provided with a hole for placing a centrifugal tube filled with bacterial liquid;
the cold end of the semiconductor refrigeration sheet is connected with the second heat conduction block and is used for refrigerating the second heat conduction block;
the second temperature sensor is arranged in the second heat conduction block and used for feeding back a temperature signal to control, so that the second heat conduction block maintains the set temperature;
the radiating fin is arranged below the semiconductor refrigerating fin;
and the heat radiation fan is arranged below the heat radiation fin.
7. The portable integrated biological assay device of claim 1, wherein each functional module comprises a microscope module, the microscope module comprising:
a microscope housing having a microscope optical amplification section integrated therein;
an objective lens window disposed on the microscope housing;
the illumination light source is arranged on the microscope shell and is opposite to the objective window;
and the focusing knob is arranged on the microscope shell, is adjusted during observation, and displays the amplified image on the control center.
8. The portable integrated biological testing device according to claim 7, wherein the overall outline dimensions of the microscope module are: length, width, height, 60, 140 (mm).
9. The portable integrated biological testing device of claim 1, wherein said control center is disposed on the device housing or on the user's mobile phone.
10. The portable integrated biological experiment device according to claim 1, wherein each functional module is a module of various instruments in a laboratory after miniaturization and modular design, or a module for realizing various new requirements, and the functional modules are increased or decreased according to actual needs; the functional modules developed aiming at the same functional instrument have different specific implementation modes.
CN202010556895.2A 2020-06-18 2020-06-18 Portable integrated biological experiment device Pending CN113817591A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202010556895.2A CN113817591A (en) 2020-06-18 2020-06-18 Portable integrated biological experiment device
PCT/CN2020/097780 WO2021253471A1 (en) 2020-06-18 2020-06-23 Portable integrated biological experiment apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010556895.2A CN113817591A (en) 2020-06-18 2020-06-18 Portable integrated biological experiment device

Publications (1)

Publication Number Publication Date
CN113817591A true CN113817591A (en) 2021-12-21

Family

ID=78911565

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010556895.2A Pending CN113817591A (en) 2020-06-18 2020-06-18 Portable integrated biological experiment device

Country Status (2)

Country Link
CN (1) CN113817591A (en)
WO (1) WO2021253471A1 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140377843A1 (en) * 2013-06-23 2014-12-25 King Abdulaziz City For Science And Technology Automated microbiology laboratory instrument and system uses thereof
CN205347432U (en) * 2015-12-24 2016-06-29 中国科学院深圳先进技术研究院 Little biological assay device of modularization
CN106554917A (en) * 2016-03-30 2017-04-05 厦门医学高等专科学校 Portable biometric precision detecting instrument and detection method
CN206892438U (en) * 2017-07-12 2018-01-16 徐州昊洋生物科技有限公司 Portable microscope equipment based on transmission of wireless signals and control
CN209890607U (en) * 2019-02-15 2020-01-03 赛慕特生物工程(上海)有限公司 Cell cryopreserving bag dry-type resuscitation device
CN210626147U (en) * 2019-05-14 2020-05-26 河南知微生物工程有限公司 Sample pretreatment integration box

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8342332B2 (en) * 2011-03-09 2013-01-01 Munirah Abdullah Alhajri Portable laboratory for a biology course and experiments
CN202570197U (en) * 2012-04-29 2012-12-05 西南民族大学 Portable biomedicine experiment box
CN103376310A (en) * 2012-04-30 2013-10-30 西南民族大学 Portable parazoon examination box
CN103381375A (en) * 2012-05-01 2013-11-06 西南民族大学 Portable biomedical experiment box
CN204276006U (en) * 2014-11-21 2015-04-22 东北林业大学 Herborization experiment integrating device
CN206139095U (en) * 2016-08-29 2017-05-03 天津市康婷生物工程有限公司 Portable placing in horizontal shaking table of 4 DEG C of refrigerators
CN107945620A (en) * 2017-11-18 2018-04-20 陕西晶辉体育设施工程有限公司 A kind of teaching is with portable biometric experimental box
CN208136260U (en) * 2018-03-17 2018-11-23 淮安市疾病预防控制中心 A kind of field Vector carries the experimental provision of pathogen Rapid identification

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140377843A1 (en) * 2013-06-23 2014-12-25 King Abdulaziz City For Science And Technology Automated microbiology laboratory instrument and system uses thereof
CN205347432U (en) * 2015-12-24 2016-06-29 中国科学院深圳先进技术研究院 Little biological assay device of modularization
CN106554917A (en) * 2016-03-30 2017-04-05 厦门医学高等专科学校 Portable biometric precision detecting instrument and detection method
CN206892438U (en) * 2017-07-12 2018-01-16 徐州昊洋生物科技有限公司 Portable microscope equipment based on transmission of wireless signals and control
CN209890607U (en) * 2019-02-15 2020-01-03 赛慕特生物工程(上海)有限公司 Cell cryopreserving bag dry-type resuscitation device
CN210626147U (en) * 2019-05-14 2020-05-26 河南知微生物工程有限公司 Sample pretreatment integration box

Also Published As

Publication number Publication date
WO2021253471A1 (en) 2021-12-23

Similar Documents

Publication Publication Date Title
CN205049811U (en) Portable microscope device
CN100535644C (en) Micro-nano system fluid chip detection system and detection method
CN112094743A (en) Living cell culture and real-time observation system and method
CN108624493A (en) Integrated DNA analysis instrument
CN111004708A (en) PCR temperature cycle control method and rotary drive type PCR temperature cycle control device
US20120140056A1 (en) Sample imaging system and method for transmitting an image of cells or tissues located in a culturing space to data prcessing means
CN113817591A (en) Portable integrated biological experiment device
CN212974933U (en) Portable constant-temperature blending instrument
CN108828249A (en) A kind of sample adding device
CN115747044A (en) Rapid temperature control device based on microfluidic chip cell culture
CN207380424U (en) Photograph taking auxiliary device and filming apparatus
CN210419975U (en) Medical treatment is with biological gene composition analysis appearance
CN213085988U (en) Microbial colony counter
JP4117341B2 (en) Hot water circulation microscope culture chamber
CN209894715U (en) Portable body fluid detection device convenient to position control
CN214974129U (en) Constant temperature culture oscillator with high-rotating-speed driving device
CN2798122Y (en) Portable digital microscope
CN218435671U (en) Microbial detection is with shaking table
CN216208447U (en) Intelligent cell analysis system
CN215560359U (en) Portable bioreactor control by temperature change base
CN212247084U (en) High content cell observation culture apparatus
CN210420131U (en) Coating device for C-lens
CN210072951U (en) Specimen holder
CN218910357U (en) Tissue grinding tool for extracting intestinal cells of shrimps
CN218539715U (en) Biochemical product culture apparatus

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination