CN113785915A - Sweet taste sensitive food formula and construction method of animal model thereof - Google Patents
Sweet taste sensitive food formula and construction method of animal model thereof Download PDFInfo
- Publication number
- CN113785915A CN113785915A CN202110894590.7A CN202110894590A CN113785915A CN 113785915 A CN113785915 A CN 113785915A CN 202110894590 A CN202110894590 A CN 202110894590A CN 113785915 A CN113785915 A CN 113785915A
- Authority
- CN
- China
- Prior art keywords
- food
- carbohydrate
- protein
- sensitivity
- drosophila
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013305 food Nutrition 0.000 title claims abstract description 56
- 235000019605 sweet taste sensations Nutrition 0.000 title claims abstract description 29
- 238000010171 animal model Methods 0.000 title claims abstract description 13
- 238000010276 construction Methods 0.000 title claims abstract description 7
- 238000009472 formulation Methods 0.000 title description 4
- 230000035945 sensitivity Effects 0.000 claims abstract description 52
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 42
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 38
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 38
- 241000255588 Tephritidae Species 0.000 claims abstract description 25
- 235000016709 nutrition Nutrition 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 17
- 241000255601 Drosophila melanogaster Species 0.000 claims abstract description 15
- 230000035764 nutrition Effects 0.000 claims abstract description 14
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims description 26
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- 235000005911 diet Nutrition 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 8
- 235000003642 hunger Nutrition 0.000 claims description 7
- 210000001364 upper extremity Anatomy 0.000 claims description 6
- 241000282414 Homo sapiens Species 0.000 claims description 5
- 230000037213 diet Effects 0.000 claims description 4
- 230000000378 dietary effect Effects 0.000 claims description 4
- 230000037351 starvation Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000002435 rhinoplasty Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 235000014633 carbohydrates Nutrition 0.000 description 31
- 230000004044 response Effects 0.000 description 10
- 241000255925 Diptera Species 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000021147 sweet food Nutrition 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 206010061428 decreased appetite Diseases 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008447 perception Effects 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 235000021403 cultural food Nutrition 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000009699 differential effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000020595 eating behavior Effects 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 108091005708 gustatory receptors Proteins 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000035918 sweet taste sensation Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/30—Dietetic or nutritional methods, e.g. for losing weight
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention discloses a food nutrition formula for improving organism sweet taste sensitivity, which utilizes a fruit fly model and a nutrition geometric framework to systematically evaluate main nutrients in food for the first time: influence of protein and carbohydrate on sweetness sensitivity, and finding an optimal food nutrition formula for improving sweetness sensitivity, wherein the mass ratio of protein to carbohydrate is 1: (1-2). The invention also provides a construction method of the animal model with high sweet taste sensitivity and a method for detecting the ratio of protein and carbohydrate to improve the sweet taste sensitivity of drosophila melanogaster.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a sweet taste sensitive dietary formula and a construction method of an animal model thereof.
Background
Sweetness sensitivity refers to the level of body perception of sweetness. Many foods rich in nutrients and energy are sweet in nature, and therefore the sweet taste sensation is very important for guiding humans and various animals in food selection and energy intake. People and various animals naturally like sweet foods, and the weakening or the lack of sweet taste sensitivity can cause the interest of the body to the sweet foods to be reduced, and the sweet foods are often generated in metabolism-related diseases such as inappetence, anorexia, diabetes and the like and depression of the body, so that the improvement of the sweet taste sensitivity of the body is beneficial to enhancing the appetite of the body, promoting the ingestion and helping to maintain the normal nutrition and metabolism level of the body.
The body's sweetness sensitivity affects the perception of food and, at the same time, can be altered by food. Some studies in humans, mice and drosophila now find that food can significantly up-regulate or down-regulate the sweet taste sensitivity of the body, suggesting the potential for the use of dietary modulation in improving the sweet taste sensitivity of the body. It is not clear what food ingredients affect sweetness sensitivity, nor has it been analyzed how the main nutrients in a food affect sweetness sensitivity in combination with the optimal food formulation that affects sweetness sensitivity.
Drosophila melanogaster (Drosophila melanogaster) is an ideal animal model for studying how food improves sweetness sensitivity and sweet taste control mechanisms. The drosophila melanogaster has a taste sensing system and sweet taste similar to those of human, taste receptor neurons are distributed on the lips, legs and wings of the drosophila melanogaster, sweet taste can be sensed, the long lips of the drosophila melanogaster are generally zoomed in and out of the head, and when the drosophila melanogaster is stimulated by food such as sweet substances, the long lips stretch and open, eating behaviors are clear and visible, so that the physiological structure of the drosophila melanogaster is very convenient for researching sweet taste sensitivity and taste preference. In addition, the drosophila has clear genetic background and rich genetic and neurological research tools, and is a mature, controllable and easily obtained animal with a physiological and pharmacological research model.
Disclosure of Invention
The invention aims to utilize a drosophila model and a nutrition geometric framework to uniformly evaluate the influence of main nutrient proteins and carbohydrates in food on sweetness sensitivity, find out an optimal food formula capable of effectively improving the sweetness sensitivity of organisms and provide a construction method of an animal model with high sweetness sensitivity.
The technical scheme adopted by the invention is as follows:
according to the first aspect of the invention, the food nutrition formula for improving the sweetness sensitivity of the organism is provided, and the mass ratio of protein to carbohydrate in the food nutrition formula is (0.8-1): (1-2.5).
Further, the organism is a human, a mouse or a fruit fly.
Preferably, the mass ratio of protein to carbohydrate in the food nutrition formula is (0.9-1): (1.8-2), more preferably 1: 2.
In a second aspect of the present invention, there is provided a dietary method for improving the sweetness sensitivity of an organism, wherein the mass ratio of ingested protein to carbohydrate is (0.8 to 1): (1-2.5).
Further still, the organism is preferably a human, mouse or fruit fly.
Preferably, the mass ratio of protein to carbohydrate in the food nutrition formula is (0.9-1): (1.8-2), more preferably 1: 2.
The third aspect of the invention provides a construction method of an animal model with high sweet taste sensitivity, wherein the mass ratio of protein to carbohydrate in a food formula of the animal model is (0.8-1): (1-2.5).
Further, the organism is a mouse or a drosophila.
Preferably, the mass ratio of protein to carbohydrate in the food formula of the animal model is (0.9-1): (1.8-2); more preferably 1: 2.
In a fourth aspect of the invention, a method for detecting the ratio of protein and carbohydrate to improve the sweet taste sensitivity of drosophila is provided, which comprises the following steps:
s1, feeding the fruit flies with food with a target index proportion in groups, wherein the index proportion is the proportion of protein and carbohydrate;
s2, detecting the influence of the food with the target index proportion on the sweet taste sensitivity of the fruit flies through a nose-growing kiss reaction test of the fruit flies.
Further, the specific method of the rhinoplasty reaction test of the drosophila melanogaster in step S2 is as follows:
transferring the fruit flies to a container without food;
after 4 hours of starvation, the drosophila were anesthetized and mounted on a glass slide;
after 6 hours of hunger, the drosophila melanogaster front legs were touched twice with drops of sucrose solution, with 1 second interval each time, and the number of individuals producing kiss reaction was recorded and the reaction rate was calculated.
Further, the flies were anesthetized by ice.
Further, the method according to claim 8, wherein the drosophila forelegs are dabbed with a gradient concentration of drops of sucrose solution.
Preferably, the sucrose solutions of gradient concentration are sucrose solutions of 6.25, 12.5, 25, 50, 100, 200, 400, 800(mM), respectively.
Furthermore, the front legs of the drosophila are lightly touched by drops of sucrose solution, and individuals who respond to water are first rejected.
The invention has the beneficial effects that:
the method utilizes the fruit fly model and the nutrition geometric framework to systematically evaluate the main nutrients in the food for the first time: the effect of proteins and carbohydrates on sweetness sensitivity and finding the best food nutritional formula to improve sweetness sensitivity. Wherein the food contains protein and carbohydrate components and the ratio of protein content is 1: (1-2). Specifically, for the model organism drosophila melanogaster, the food contains protein and carbohydrate, the protein content is 80-100 g/L, the carbohydrate content is 100-250g/L, the sweet taste sensitivity of the organism can be obviously increased, and particularly, the food formula with the optimal sweet taste sensitivity enhancing effect is provided with the protein-carbohydrate ratio of 1: 2.
Drawings
FIG. 1 the response of Drosophila to sucrose solutions of different concentrations at different carbohydrate/protein ratios.
FIG. 2 shows the distribution of the half-number of sucrose-sensitive concentrations of Drosophila at different carbohydrate/protein ratios.
FIG. 3 weight and hunger survival time distribution of Drosophila at different carbohydrate/protein ratios.
Detailed Description
In order to clearly understand the technical contents of the present invention, the following embodiments are described in detail with reference to the accompanying drawings. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, for which specific conditions are not noted in the following examples, are generally carried out under the conditions described in the conventional conditions or under the conditions recommended by the manufacturers. The various chemicals used in the examples are commercially available.
Examples
Animal model: wild type fruit fly W1118。
Culturing and amplifying a large amount of the strain of drosophila melanogaster according to a standard process (W)1118) The constant temperature and humidity incubator for experimental feeding of the fruit flies has the following parameters: temperature: 25 +/-1 ℃; relative humidity 65% ± 5%; day and night circulation: 12h of illumination (7: 30-19: 30)/12h of darkness (19: 30-7: 30).
Experimental reagent:
TABLE 1 Experimental reagent Table
Conventional food formula for raising and breeding experimental drosophila: each 1L of the diet contained water, agar (1%), yeast powder (10%), sucrose (5%), propionic acid (0.6%), 10% m/v methyl paraben-ethanol solution (1.2%).
The sweet taste sensitivity detection method comprises the following steps:
the sweet taste sensitivity of Drosophila melanogaster can be measured by the Probossis Extension Response (PER) (Shiraiwa T, Carlson JR. Probossis Extension Response (PER) assay in Drosophila. journal of visual extensions: JoVE,2007, (3): 193-). PER is an instinctive response of fruit flies extending out of the lips after being specifically stimulated by the outside world and was used in this study to test the sensitivity of fruit flies to sweetness. The level of sweetness sensitivity was quantified by taking the number of tickle responses to each concentration of solution in a group of flies (typically 10-12) and calculating the ratio, and the level of sweetness sensitivity was measured as the ratio, usually expressed as a percentage, after stimulating the flies with a series of graded concentrations of sucrose solution from low to high. A high sensitivity means that a higher proportion of Drosophila produces a kissing response and vice versa.
The effect of protein and carbohydrate sensitivity to sweetness was studied using a nutritional geometry framework:
the total flow is as follows: a plurality of foods with different protein and carbohydrate contents and proportions are prepared, after the flies are fed for a period of time, the sweet taste sensitivity of the flies is detected, and data analysis and response surface mapping are carried out on the results by using R.
In order to investigate the effect of major nutrient proteins (proteins, P) and carbohydrates (C) and the ratio between them (P: C) on the sweet taste sensitivity of Drosophila melanogaster, a two-dimensional nutrient geometric framework food consisting of 28 different Protein and carbohydrate contents and ratios of foods (Lee KP, Simpson SJ, Clissold FJ, et al. Life and reproduction in Drosophila: New instructions from nutritional geometry. proceedings of the National Academy of Sciences of the United States of America,2008,105(7): 2498. 2503.) was used in experiments in which seven Protein and carbohydrate ratios were included (P: C: 0:1, 1:16, 1:8, 1:4, 1:2, 1:1, 9: 90, and four total energy levels (180 g/360). The formulation of these 28 diets is shown in table 2 below.
Table 228 kinds food formula table
Remarking: the yeast contains 45% of protein and 24% of carbohydrate; each 1L of the food contained 1% agar, 1.2% methyl paraben 10% solution, and 0.6% propionic acid.
Healthy male W1118 fruit flies of 4-7 days old, which developed on regular food, were selected, 35 flies in each group, and transferred to 28 groups of food, respectively, and cultured for 6 days. Sweetness sensitivity was performed by the detection-PER test on day seven.
The specific process of performing the sweet taste sensitivity test on the drosophila after each group of food treatment is as follows:
9: 00-10:00 AM: flies were transferred to fly tubes containing no food, only wet paper towels soaked with 2ml water, and started to starve.
1: 00-2:00 PM: after 4h of starvation, the flies were anesthetized with ice and fixed back on a glass slide with nail polish and tweezers. Each row is fixed with 12 pieces, and three rows are fixed. The slide with the adhered fruit fly is then placed in a wet box and returned to the incubator to allow the fruit fly to wake up.
3: 00-4: 00 PM: after 6h of starvation, the same batch of fruit flies was tested for PER within 1 h. The test process is as follows: drosophila were first tested under a microscope with water, and individuals who responded to water were excluded. The drosophila forelegs were then tapped twice with drops of 6.25, 12.5, 25, 50, 100, 200, 400, 800(mM) sucrose solution, each time at 1 second intervals, and the number of individuals producing the kissing reaction was recorded and the rate of reaction calculated. This experiment every group sets up three parallel experiment, and every parallel contains 10 ~ 12 fruit flies, guarantees that every parallel is rejected and is greater than 8 to the individual back quantity of water-sensitive.
This gave data on the effect of 28 different foods of carbohydrate and protein content and ratio on the sweet taste sensitivity of drosophila (statistics see table 3). Finally, PER experimental data were processed by Excel and R (3.6.3) to generate a protein/carbohydrate nutrient geometry-sweetness sensitivity response profile, and the results are shown in fig. 1 and 2.
TABLE 328 Effect of different carbohydrate and protein content and ratio of foods on Drosophila sweetness sensitivity
In addition, the inventor also measured the body weight of drosophila after culturing for 6 days with 28 kinds of food (6 repeated samples, statistical results are shown in table 4) and the hunger-tolerant survival time in the state without food (12 repeated samples, statistical results are shown in table 5), and obtained a protein/carbohydrate nutrition geometry-body weight response surface graph and a protein/carbohydrate nutrition geometry-hunger survival time response surface graph, and the results are shown in fig. 1 and 2.
Table 428 weight of fruit fly after 6 days of food culture
Body weight (g) | M1 | M2 | M3 | M4 | M5 | M6 | Total up to | Mean value of |
1# | 0.0077 | 0.0074 | 0.008 | 0.0079 | 0.0076 | 0.0078 | 0.0464 | 0.007733 |
2# | 0.0081 | 0.008 | 0.0082 | 0.0081 | 0.008 | 0.008 | 0.0484 | 0.008067 |
3# | 0.0084 | 0.0085 | 0.0083 | 0.0084 | 0.0082 | 0.0087 | 0.0505 | 0.008417 |
4# | 0.0086 | 0.0086 | 0.0085 | 0.0083 | 0.0085 | 0.0083 | 0.0508 | 0.008467 |
5# | 0.0092 | 0.0087 | 0.0085 | 0.0083 | 0.0085 | 0.0085 | 0.0517 | 0.008617 |
6# | 0.0086 | 0.0087 | 0.0084 | 0.0083 | 0.0076 | 0.0078 | 0.0494 | 0.008233 |
7# | 0.0079 | 0.0081 | 0.0081 | 0.0073 | 0.007 | 0.007 | 0.0454 | 0.007567 |
8# | 0.0077 | 0.008 | 0.0081 | 0.0079 | 0.0076 | 0.0082 | 0.0475 | 0.007917 |
9# | 0.0083 | 0.0081 | 0.0082 | 0.0079 | 0.0081 | 0.0081 | 0.0487 | 0.008117 |
10# | 0.0082 | 0.0084 | 0.0085 | 0.0084 | 0.0083 | 0.0082 | 0.05 | 0.008333 |
11# | 0.0087 | 0.0088 | 0.0086 | 0.0082 | 0.0086 | 0.0085 | 0.0514 | 0.008567 |
12# | 0.0088 | 0.0085 | 0.009 | 0.009 | 0.0085 | 0.0088 | 0.0526 | 0.008767 |
13# | 0.0086 | 0.0087 | 0.0085 | 0.0082 | 0.0086 | 0.0085 | 0.0511 | 0.008517 |
14# | 0.0081 | 0.0082 | 0.0082 | 0.008 | 0.0077 | 0.0076 | 0.0478 | 0.007967 |
15# | 0.008 | 0.0079 | 0.0081 | 0.0078 | 0.0078 | 0.0077 | 0.0473 | 0.007883 |
16# | 0.0086 | 0.0081 | 0.0084 | 0.0082 | 0.0084 | 0.008 | 0.0497 | 0.008283 |
17# | 0.0084 | 0.0084 | 0.0084 | 0.0084 | 0.0084 | 0.0082 | 0.0502 | 0.008367 |
18# | 0.0086 | 0.0086 | 0.0086 | 0.0086 | 0.0079 | 0.008 | 0.0503 | 0.008383 |
19# | 0.0088 | 0.0083 | 0.0085 | 0.0085 | 0.0082 | 0.0087 | 0.051 | 0.0085 |
20# | 0.0087 | 0.0086 | 0.0084 | 0.0087 | 0.0085 | 0.0086 | 0.0515 | 0.008583 |
22# | 0.008 | 0.0079 | 0.0079 | 0.0077 | 0.008 | 0.0075 | 0.047 | 0.007833 |
23# | 0.0082 | 0.0084 | 0.0083 | 0.0081 | 0.0082 | 0.008 | 0.0492 | 0.0082 |
24# | 0.0087 | 0.0087 | 0.0085 | 0.0081 | 0.0086 | 0.0084 | 0.051 | 0.0085 |
25# | 0.0088 | 0.0085 | 0.0087 | 0.0087 | 0.0086 | 0.0087 | 0.052 | 0.008667 |
26# | 0.0087 | 0.0089 | 0.0088 | 0.0075 | 0.0096 | 0.0088 | 0.0523 | 0.008717 |
TABLE 5 statistics of starvation-tolerant survival time in the diet free state
As can be seen from FIGS. 1-2, the six gradient sucrose solution stimulations of 6.26-200mM, the food containing different contents and proportions of protein and carbohydrate have a significant differential effect on the sweet taste sensitivity of Drosophila. High protein and low carbohydrate foods contribute to increased sweetness sensitivity. Particularly, when the protein content is 80-100 g/L and the carbohydrate content is within the range of 100-250g/L, the sweetness sensitivity can be obviously increased; furthermore, when the protein content is 90-100 g/L and the carbohydrate content is within the range of 180-200g/L, the sweetness sensitivity can be increased more remarkably; when the ratio of P to C is 1:2, the sweetness sensitivity induced by the food was the strongest (p < 0.001). By way of assistance, it can be seen from figure 3 that there is a relatively optimal weight accumulation and starvation-tolerant survival at this range, i.e. optimal ratio.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (10)
1. A food nutrition formula for improving the sweetness sensitivity of organisms is characterized in that the mass ratio of protein to carbohydrate in the food nutrition formula is (0.8-1): (1-2.5); the organism is preferably a human, mouse or fruit fly.
2. The food nutritional formula according to claim 1, wherein the mass ratio of protein to carbohydrate in the food nutritional formula is (0.9-1): (1.8-2), more preferably 1: 2.
3. A diet method for improving the sweetness sensitivity of an organism is characterized in that the mass ratio of ingested protein to carbohydrate is (0.8-1): (1-2.5); the organism is preferably a human, mouse or fruit fly.
4. A dietary method according to claim 3, wherein the mass ratio of ingested protein to carbohydrate is (0.9-1): (1.8-2), more preferably 1: 2.
5. A method for constructing an animal model with high sweet taste sensitivity is characterized in that the mass ratio of protein to carbohydrate in a food formula of the animal model is (0.8-1): (1-2.5); the organism is preferably a mouse or a fruit fly.
6. The construction method according to claim 5, wherein the mass ratio of protein to carbohydrate in the food formula of the animal model is (0.9-1): (1.8-2); more preferably 1: 2.
7. A method for detecting the sensitivity of the ratio of protein and carbohydrate to improve the sweetness of drosophila, comprising the steps of:
s1, feeding the fruit flies with food with a target index proportion in groups, wherein the index proportion is the proportion of protein and carbohydrate;
s2, detecting the influence of the food with the target index proportion on the sweet taste sensitivity of the fruit flies through a nose-growing kiss reaction test of the fruit flies.
8. The method as claimed in claim 7, wherein the specific method of the rhinoplasty test of drosophila in step S2 is as follows:
transferring the fruit flies to a container without food;
after 4 hours of starvation, the drosophila were anesthetized and mounted on a glass slide;
after 6 hours of hunger, the drosophila melanogaster front legs were touched twice with drops of sucrose solution, with 1 second interval each time, and the number of individuals producing kiss reaction was recorded and the reaction rate was calculated.
9. The method of claim 8, wherein the drosophila forelegs are dabbed with a gradient concentration drop of sucrose solution.
10. The method of claim 9, wherein the gradient concentration sucrose solutions are 6.25, 12.5, 25, 50, 100, 200, 400, 800(mM) sucrose solutions, respectively.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110894590.7A CN113785915A (en) | 2021-08-05 | 2021-08-05 | Sweet taste sensitive food formula and construction method of animal model thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110894590.7A CN113785915A (en) | 2021-08-05 | 2021-08-05 | Sweet taste sensitive food formula and construction method of animal model thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113785915A true CN113785915A (en) | 2021-12-14 |
Family
ID=78877135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110894590.7A Pending CN113785915A (en) | 2021-08-05 | 2021-08-05 | Sweet taste sensitive food formula and construction method of animal model thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113785915A (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0132444A1 (en) * | 1983-07-13 | 1985-02-13 | General Foods Corporation | Sweetening composition and process for modifying the sweetness perception of a foodstuff |
CN101175416A (en) * | 2005-04-06 | 2008-05-07 | 诺瓦提斯公司 | A method and composition for nutritionally improving glucose control and insulin action |
JP2015192664A (en) * | 2014-03-28 | 2015-11-05 | 味の素株式会社 | Sweet taste receptor chimeric protein and use thereof |
US20160029682A1 (en) * | 2014-08-01 | 2016-02-04 | Mead Johnson Nutrition Company | Hydrolyzed lactose-containing nutritional compositions and uses thereof |
-
2021
- 2021-08-05 CN CN202110894590.7A patent/CN113785915A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0132444A1 (en) * | 1983-07-13 | 1985-02-13 | General Foods Corporation | Sweetening composition and process for modifying the sweetness perception of a foodstuff |
CN101175416A (en) * | 2005-04-06 | 2008-05-07 | 诺瓦提斯公司 | A method and composition for nutritionally improving glucose control and insulin action |
JP2015192664A (en) * | 2014-03-28 | 2015-11-05 | 味の素株式会社 | Sweet taste receptor chimeric protein and use thereof |
US20160029682A1 (en) * | 2014-08-01 | 2016-02-04 | Mead Johnson Nutrition Company | Hydrolyzed lactose-containing nutritional compositions and uses thereof |
Non-Patent Citations (1)
Title |
---|
QIAO-PING WANG等: "PGC1a Controls Sucrose Taste Sensitization in Drosophila" * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Dalle Zotte et al. | Black soldier fly (Hermetia illucens) as dietary source for laying quails: live performance, and egg physico-chemical quality, sensory profile and storage stability | |
Tamayo et al. | Physiological basis of extreme growth rate differences in the spat of oyster (Crassostrea gigas) | |
Yan et al. | Feed restriction induced changes in behavior, corticosterone, and microbial programming in slow-and fast-growing chicken breeds | |
DeGrandi-Hoffman et al. | The importance of time and place: nutrient composition and utilization of seasonal pollens by European honey bees (Apis mellifera L.) | |
Han et al. | Effects of dietary stevioside supplementation on feed intake, digestion, ruminal fermentation, and blood metabolites of goats | |
Liu et al. | Glycerol monolaurate enhances reproductive performance, egg quality and albumen amino acids composition in aged hens with gut microbiota alternation | |
Daneshmand et al. | Effect of purine nucleosides on growth performance, gut morphology, digestive enzymes, serum profile and immune response in broiler chickens | |
Wang et al. | The effect of Camellia oleifera cake polysaccharides on growth performance, carcass traits, meat quality, blood profile, and caecum microorganisms in yellow broilers | |
Panda et al. | Nutritional evaluation and utilisation of quality protein maize, Nityashree hybrid maize, and normal maize in broiler chickens | |
Brereton | Challenges and directions in zoo and aquarium food presentation research: A review | |
Zhang et al. | Effects of lard and vegetable oils supplementation quality and concentration on laying performance, egg quality and liver antioxidant genes expression in Hy-line Brown | |
Tsala et al. | Effects of dried olive pulp dietary supplementation on quality characteristics and antioxidant capacity of pig meat | |
Herz et al. | More power with flower for the pupal parasitoid Trichopria drosophilae: A candidate for biological control of the Spotted Wing Drosophila | |
Janmohammadi et al. | The energy value for broiler chickens of heat-treated and untreated amaranth grain, with and without enzyme addition | |
Meherunnahar et al. | Development of novel foxtail millet-based nutri-rich instant noodles: Chemical and quality characteristics | |
Ghramh et al. | Honey bees prefer pollen substitutes rich in protein content located at short distance from the apiary | |
Chen et al. | Study on the quality of mixed silage of rapeseed with alfalfa or myriophyllum | |
CN109988725A (en) | The preparation technique and application of reducing weight and blood fat microbial bacterial agent and its derivative | |
Ye et al. | Feed restriction improves lipid metabolism by changing the structure of the cecal microbial community and enhances the meat quality and flavor of bearded chickens | |
Azzam et al. | Threonine requirements in dietary low crude protein for laying hens under high-temperature environmental climate | |
CN113785915A (en) | Sweet taste sensitive food formula and construction method of animal model thereof | |
Alharthi et al. | Effects of feeding different levels of sprouted barley on fermentation characteristics, bacterial quantification, and rumen morphology of growing lambs | |
Kim et al. | Comparative study of the effect of pollen substitute diets on honey bees during early spring | |
Feng et al. | Feeding laying ducks Eucommia ulmoides oliv. leaves increases the n-3 fatty acids content and decreases the n-6: n-3 PUFA ratio in egg yolk without affecting laying performance or egg quality | |
Molleman et al. | Does dietary restriction reduce life span in male fruit-feeding butterflies? |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |