CN113772803A - Device for culturing free-state high-abundance complete nitrifying bacteria - Google Patents
Device for culturing free-state high-abundance complete nitrifying bacteria Download PDFInfo
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- CN113772803A CN113772803A CN202111000087.9A CN202111000087A CN113772803A CN 113772803 A CN113772803 A CN 113772803A CN 202111000087 A CN202111000087 A CN 202111000087A CN 113772803 A CN113772803 A CN 113772803A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/006—Regulation methods for biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/102—Permeable membranes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/105—Characterized by the chemical composition
- C02F3/108—Immobilising gels, polymers or the like
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/20—Activated sludge processes using diffusers
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F5/00—Softening water; Preventing scale; Adding scale preventatives or scale removers to water, e.g. adding sequestering agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/38—Gas flow rate
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a device for culturing free high-abundance complete nitrobacteria, which comprises a raw sewage tank, a membrane bioreactor, an aeration mechanism and an ammonia nitrogen regulating mechanism, wherein the membrane bioreactor comprises a reaction barrel, a polyethylene hollow fiber membrane component, a water inlet pipe and a water outlet pipe, the raw sewage tank conveys sewage with low calcium and magnesium concentration into the reaction barrel, activated sludge in the reaction barrel reacts with calcium and magnesium ions and ammonia nitrogen substrates in the sewage with low calcium and magnesium concentration, dissolved oxygen and ammonia nitrogen concentration in the reaction barrel are regulated in real time by the arranged aeration mechanism and ammonia nitrogen regulating mechanism, a uniform medium growth environment with low oxygen and ammonia nitrogen concentration is provided for the growth of the complete nitrobacteria, the effective enrichment of the free high-abundance complete nitrobacteria is realized, meanwhile, microorganisms can be intercepted to the maximum degree by the arranged polyethylene hollow fiber membrane component, and finally the cultured complete nitrobacteria can provide a test basis for researching the physiological and biochemical characteristics of the complete nitrobacteria.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a device for culturing free-state high-abundance complete nitrifying bacteria.
Background
The Russian microbiologist, Sergei Winogradsky, separated the nitrification process into two parts of microorganisms, AOB (ammonia oxidizing bacteria) oxidized ammonia nitrogen into nitrite nitrogen, then NOB (nitrite oxidizing bacteria) oxidizes nitrite nitrogen into nitrate nitrogen, the theory occupies the authoritative position of the nitrification process in the nitrogen cycle for more than one hundred years, after two papers for culturing complete nitrifying bacteria are issued in sequence from Nature in 2015, nitrification was demonstrated to be accomplished by one species of bacteria, without going through two species of bacteria, after years of research, the complete nitrifying bacteria are widely distributed around the world, more and more complete nitrifying bacteria are discovered along with the development of research technology, and the enrichment culture of the high-purity and high-abundance complete nitrifying bacteria has extremely important significance for researching the physiological and biochemical characteristics and the effect and the status in the biological denitrification process.
Many recent researches show that the complete nitrifying bacteria have competitive advantages compared with AOB and NOB in a low-substrate and low-oxygen environment, the abundance is gradually increased and dominates in long-term experiments, but at present, no pure culture of the complete nitrifying bacteria exists, the physiological and biochemical characteristics of the complete nitrifying bacteria cannot be researched, the research on the process that the complete nitrifying bacteria have the competitive advantages is not easy to develop, the free bacteria are favorable for researching the physiological and biochemical reactions of the microorganisms due to the special particle size of the free bacteria, and the research shows that the free bacteria are closer to the physiological and biochemical properties of the microorganisms and are less influenced by other environmental factors compared with other forms, and the culture of the free complete nitrifying bacteria has not been carried out by people in the years after the complete nitrifying bacteria are discovered.
Disclosure of Invention
Therefore, the invention provides a device for culturing free-state high-abundance complete nitrifying bacteria, which can be used for culturing the complete nitrifying bacteria by creating a suitable environment, and finally achieves the purpose of enriching and culturing the free-state high-abundance complete nitrifying bacteria, thereby achieving the purpose of better researching the physiological and biochemical characteristics of the complete nitrifying bacteria.
The technical scheme of the invention is realized as follows:
a device for culturing free-form high-abundance complete nitrifying bacteria comprises a raw sewage tank, a membrane bioreactor, an aeration mechanism and an ammonia nitrogen adjusting mechanism, wherein the raw sewage tank is used for storing sewage with low calcium and magnesium concentration; the aeration mechanism comprises an aeration head and a first air pump, wherein the aeration head is arranged in the reaction barrel and is connected with the first air pump through an air inlet pipe; the ammonia nitrogen adjustment mechanism comprises an upper computer and an ammonia nitrogen sensor, the water inlet pipe is provided with a water inlet pump, the ammonia nitrogen sensor is arranged inside the reaction barrel, and the upper computer is respectively electrically connected with the ammonia nitrogen sensor and the water inlet pump.
Preferably, still include aeration control mechanism, aeration control mechanism includes gas flowmeter, air quantity governing valve, dissolved oxygen sensor and dissolved oxygen controller, the dissolved oxygen sensor sets up inside the retort, air quantity governing valve and gas flowmeter all set up in the intake pipe, gas flowmeter is located between air pump and the air quantity governing valve, the dissolved oxygen controller is connected with air quantity governing valve and dissolved oxygen sensor electricity respectively.
Preferably, the sewage treatment device further comprises a switch valve, wherein a vent pipe is arranged at the bottom of the sewage raw water tank, and the switch valve is arranged on the vent pipe, the water inlet pipe and the water outlet pipe.
Preferably, the sewage treatment device further comprises an overflow pipe, wherein the overflow pipe is arranged on the side wall of the sewage raw water tank and the side wall of the reaction barrel, and the overflow pipe on the side wall of the reaction barrel is positioned above the water outlet pipe.
Preferably, still include rabbling mechanism, rabbling mechanism includes agitator motor, (mixing) shaft and stirring vane, agitator motor output shaft is connected with the (mixing) shaft top, during the (mixing) shaft bottom stretched into the reaction barrel, stirring vane sets up on the (mixing) shaft lateral wall.
Preferably, still include calcium magnesium concentration adjustment mechanism, calcium magnesium concentration adjustment mechanism includes calcium magnesium ion content detection mechanism, first breather pipe, second air pump and carbon dioxide storage tank, calcium magnesium ion content detection mechanism is used for detecting the calcium magnesium ion content of the raw water tank sewage of sewage, first breather pipe one end is stretched into raw water tank of sewage, and the other end communicates with the end of giving vent to anger of second air pump, the inlet end and the inside intercommunication of carbon dioxide storage tank of second air pump.
Preferably, the calcium and magnesium ion content detection mechanism comprises a sampling box, a sampling pipe, a first three-way electromagnetic valve, a second vent pipe, a second three-way electromagnetic valve, a calcium and magnesium concentration controller, a weighing sensor and a drainage mechanism, wherein the first three-way electromagnetic valve is arranged on the water inlet pipe, one end of the sampling pipe is connected with the first three-way electromagnetic valve, the other end of the sampling pipe is positioned above the sampling box, the second three-way electromagnetic valve is connected with a second air pump, the first vent pipe and the second vent pipe are both connected with the second three-way electromagnetic valve, the second vent pipe extends into the sampling box, the weighing sensor is arranged on the inner bottom surface of the sampling box, the drainage mechanism comprises a drainage pipe, a drainage electromagnetic valve and a filter screen, the drainage pipe is connected with the bottom surface of the sampling box, the filter screen is arranged on the inner bottom surface of the sampling box and positioned above the drainage pipe, and the drainage electromagnetic valve is arranged on the drainage pipe, the calcium-magnesium concentration controller is arranged on the outer side wall of the sampling box and is respectively and electrically connected with the first three-way electromagnetic valve, the second air pump, the weighing sensor and the water drainage electromagnetic valve.
Preferably, the calcium and magnesium concentration adjusting mechanism further comprises a gas flow sensor, the gas flow sensor is arranged in the first vent pipe and the second vent pipe, and the calcium and magnesium concentration controller is electrically connected with the gas flow sensor.
Preferably, still get rid of the mechanism including the precipitate, the mechanism is got rid of to the precipitate sets up in former water tank of sewage and sample case respectively, the mechanism is got rid of to the precipitate includes rotating electrical machines, stay cord, lifter plate and baffle, the lifter plate sets up at former water tank of sewage and sample case bottom surface, weighing sensor sets up inside the lifter plate, rotating electrical machines sets up at former water tank of sewage and sample case lateral wall top, stay cord one end winding is on the rotating electrical machines output shaft, and other end downwardly extending is connected with the lifter plate top surface, the baffle sets up in former water tank of sewage inside wall top and sample case inside wall top, and is located on the ascending path of lifter plate, calcium magnesium concentration controller is connected with the rotating electrical machines electricity, be provided with a plurality of through-holes on the lifter plate.
Preferably, the sediment removing mechanism further comprises an elevating platform, the elevating platform is arranged at the tops of the side walls of the sewage raw water tank and the sampling tank, and the rotating motor is arranged on the top surface of the elevating platform.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a device for culturing free-form high-abundance complete nitrifying bacteria, which is characterized in that low-calcium-magnesium sewage stored in a sewage raw water tank is conveyed into a membrane bioreactor, calcium-magnesium ions and ammonia nitrogen substrates in the sewage can react with activated sludge in the membrane bioreactor, and in the process, the dissolved oxygen and ammonia nitrogen concentrations in the membrane bioreactor can be regulated by an aeration mechanism and an ammonia nitrogen regulation mechanism, so that the environment in the membrane bioreactor is suitable for the growth of the complete nitrifying bacteria, meanwhile, the effect of retaining microorganisms can be furthest realized by a polyethylene hollow membrane component, a large amount of complete nitrifying bacteria can be obtained in the membrane bioreactor after long-time culture, and a test basis is provided for researching the physiological and biochemical characteristics of the complete nitrifying bacteria.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic diagram of a device for culturing free-state high-abundance complete nitrifying bacteria according to the invention;
FIG. 2 is a schematic view of a connection structure of a sampling box and a precipitate removal mechanism of the device for culturing free-state high-abundance complete nitrifying bacteria;
in the figure, 1 is a raw sewage tank, 2 is a reaction barrel, 3 is a polyethylene hollow fiber membrane component, 4 is a water inlet pipe, 5 is a water outlet pipe, 6 is an aeration head, 7 is a first air pump, 8 is an upper computer, 9 is an ammonia nitrogen sensor, 10 is a water inlet pump, 11 is a gas flowmeter, 12 is a gas flow regulating valve, 13 is a dissolved oxygen sensor, 14 is a dissolved oxygen controller, 15 is a switch valve, 16 is a blow-down pipe, 17 is an overflow pipe, 18 is a stirring motor, 19 is a stirring shaft, 20 is a stirring blade, 21 is a first vent pipe, 22 is a second air pump, 23 is a carbon dioxide storage tank, 24 is a sampling tank, 25 is a sampling pipe, 26 is a first three-way electromagnetic valve, 27 is a second vent pipe, 28 is a second three-way electromagnetic valve, 29 is a calcium magnesium concentration controller, 30 is a weighing sensor, 31 is a drain pipe, 32 is a drain electromagnetic valve, 33 is a filter screen, 34 is a gas flow sensor, reference numeral 35 denotes a rotary motor, 36 denotes a pull cord, 37 denotes a lifting plate, 38 denotes a baffle plate, 39 denotes a through hole, and 40 denotes a heightening table.
Detailed Description
For a better understanding of the technical content of the present invention, a specific embodiment is provided below, and the present invention is further described with reference to the accompanying drawings.
Referring to fig. 1 to 2, the device for culturing free-form high-abundance complete nitrifying bacteria provided by the invention comprises a raw sewage tank 1, a membrane bioreactor, an aeration mechanism and an ammonia nitrogen regulation mechanism, wherein the raw sewage tank 1 stores sewage with low calcium and magnesium concentration, the membrane bioreactor comprises a reaction barrel 2, a polyethylene hollow fiber membrane component 3, a water inlet pipe 4 and a water outlet pipe 5, activated sludge is stored in the reaction barrel 2, the water inlet pipe 4 and the water outlet pipe 5 are respectively connected with the side wall of the reaction barrel 2, and the polyethylene hollow fiber membrane component 3 is arranged in the reaction barrel 2 and is positioned on one side of the water outlet pipe 5; the aeration mechanism comprises an aeration head 6 and a first air pump 7, wherein the aeration head 6 is arranged inside the reaction barrel 2 and is connected with the first air pump 7 through an air inlet pipe; the ammonia nitrogen adjustment mechanism comprises an upper computer 8 and an ammonia nitrogen sensor 9, the water inlet pipe 4 is provided with a water inlet pump 10, the ammonia nitrogen sensor 9 is arranged inside the reaction barrel 2, and the upper computer 8 is respectively electrically connected with the ammonia nitrogen sensor 9 and the water inlet pump 10.
The invention relates to a device for culturing free-state high-abundance complete nitrifying bacteria, which is mainly characterized in that the culture environment of the complete nitrifying bacteria is controlled, so that the complete nitrifying bacteria can grow in the most suitable environment, a large amount of complete nitrifying bacteria can be obtained, and a test basis is provided for researching the physiological and biochemical characteristics of the complete nitrifying bacteria, wherein the water inlet formula for culturing the complete nitrifying bacteria is improved, the calcium and magnesium element concentration which is favorable for producing extracellular polymeric substances in the sewage stored in a raw sewage tank 1 is reduced, the complete nitrifying bacteria have the characteristics of high activity and low aggregation, the complete nitrifying bacteria are developed to a free state, the sewage with low calcium and magnesium concentration enters a membrane bioreactor through a water inlet pipe 4, and a good and stable uniform medium growth environment with low oxygen and ammonia nitrogen concentration is formed in the membrane bioreactor through the control of an aeration mechanism and an ammonia nitrogen regulation mechanism, is suitable for the growth and enrichment of the complete nitrifying bacteria, realizes the effective enrichment of the free-state high-abundance complete nitrifying bacteria, and can obtain the high-purity free-state complete nitrifying bacteria.
When nitrobacteria culture is carried out, sewage in a sewage raw water tank 1 is introduced into a reaction barrel 2 through a water inlet pipe 4, calcium magnesium ions and an ammonia nitrogen substrate contained in the sewage are contacted with activated sludge in the reaction barrel 2, the activated sludge reacts with the ammonia nitrogen substrate, dissolved oxygen and the ammonia nitrogen substrate in the reaction barrel 2 descend due to reaction consumption, external air is input into the reaction barrel 2 through an aeration head 6 by a first air pump 7, adjustment of dissolved oxygen concentration is achieved, meanwhile, the ammonia nitrogen sensor 9 can detect the ammonia nitrogen concentration in the reaction barrel 2, then the upper computer 8 can control the opening of a water inlet pump 10, the sewage is supplemented into the reaction barrel 2, and a proper environment is provided for nitrobacteria culture.
The reaction barrel 2 is also internally provided with a polyethylene hollow fiber membrane component 3, the polyethylene hollow fiber membrane component 3 is arranged between the water inlet pipe 4 and the water outlet pipe 5 and is positioned on one side of the water outlet pipe 5, when the water inlet pipe 4 conveys sewage into the reaction barrel 2, the sewage in the reaction barrel 2 can pass through the polyethylene hollow fiber membrane component 3 and flow out of the water outlet pipe 5, the arranged polyethylene hollow fiber membrane component 3 can intercept microorganisms to the greatest extent, meanwhile, uniform-speed drainage can be realized, and the inhibition effect of the generated nitrate nitrogen accumulation on complete nitrobacteria is avoided.
The culture steps of the invention are as follows:
1) starting the system: inoculating sludge in a sand filter sedimentation tank of a water works and adding the sludge to a membrane bioreactor to ensure that the sludge concentration is 2000-4000 mg/L;
2) the runtime adjustment operation is as follows:
2.1) controlling the sludge age of the membrane bioreactor to be 10-40 days, controlling the dissolved oxygen to be 0.02-0.07mg/L, controlling the ammonia nitrogen concentration in the reactor to be 0-5mg/L, controlling the hydraulic retention time to be 0.5-1d and controlling the pH to be 7.5 +/-0.5;
2.2) the fiber aperture of the polyethylene hollow fiber membrane component 3 is 0.1um, which can intercept microbial cells and make the membrane bioreactor have longer sludge age;
2.3) the aeration mechanism regulates and controls the dissolved oxygen in the membrane bioreactor;
2.4) the ammonia nitrogen concentration and the calcium magnesium ion concentration are respectively regulated and controlled by the regulation and control of an upper computer 8 and the improvement of a water inlet formula, wherein the ammonia nitrogen concentration range is 0-5mg/L, the calcium ion concentration range is 0.01-0.02mg/L, and the magnesium ion concentration range is 0.02-0.04 mg/L.
Preferably, the system further comprises an aeration control mechanism, the aeration control mechanism comprises a gas flow meter 11, a gas amount regulating valve 12, a dissolved oxygen sensor 13 and a dissolved oxygen controller 14, the dissolved oxygen sensor 13 is arranged inside the reaction barrel 2, the gas amount regulating valve 12 and the gas flow meter 11 are both arranged on the gas inlet pipe, the gas flow meter 11 is positioned between the gas pump and the gas amount regulating valve 12, and the dissolved oxygen controller 14 is electrically connected with the gas amount regulating valve 12 and the dissolved oxygen sensor 13 respectively.
In order to ensure the concentration of the dissolved oxygen in the reaction barrel 2 to be constant, the invention is provided with an aeration control mechanism, the dissolved oxygen sensor 13 can detect the concentration of the dissolved oxygen in the reaction barrel 2, and when the concentration of the dissolved oxygen is lower, the dissolved oxygen controller 14 increases the air inflow by controlling the air quantity regulating valve 12; when the dissolved oxygen concentration is higher than the control range, the aeration is preferentially stopped, when the dissolved oxygen concentration is not reduced to the target range within 5min after the aeration is stopped, the pump speed of the water inlet pump 10 is increased, the substrate concentration is increased, the situation that the substrate concentration is insufficient due to the increase of growth biomass is reduced, when the dissolved oxygen concentration is recovered to the target concentration range, the pump speed of the water inlet pump 10 is preferentially recovered, the aeration amount is gradually reduced, then the operation is carried out again, the above actions are repeated, and finally the purposes of controlling the dissolved oxygen, the substrate concentration and the calcium and magnesium ion concentration in the reaction barrel 2 to be within the target range, providing a stable living environment for complete nitrobacteria and achieving the purpose of enriching and culturing the free high-abundance complete nitrobacteria are achieved.
Preferably, the device further comprises a switch valve 15, the bottom of the raw sewage tank 1 is provided with an emptying pipe 16, and the switch valve 15 is arranged on the emptying pipe 16, the water inlet pipe 4 and the water outlet pipe 5.
The switch valve 15 is arranged to realize the opening and closing of the emptying pipe 16, the water inlet pipe 4 and the water outlet pipe 5.
Preferably, the device also comprises an overflow pipe 17, wherein the overflow pipe 17 is arranged on the side wall of the raw sewage tank 1 and the side wall of the reaction barrel 2, and the overflow pipe 17 on the side wall of the reaction barrel 2 is positioned above the water outlet pipe 5.
When the liquid level in the raw sewage tank 1 and the reaction tank 2 is high, the liquid flows out through the overflow pipe 17.
Preferably, still include rabbling mechanism, rabbling mechanism includes agitator motor 18, (mixing) shaft 19 and stirring vane 20, agitator motor 18 output shaft is connected with (mixing) shaft 19 top, 19 bottom of (mixing) shaft stretch into in the reaction barrel 2, stirring vane 20 sets up on 19 lateral walls of (mixing) shaft.
Can realize the stirring to the mixture in the reaction vessel 2 through the rabbling mechanism that sets up, agitator motor 18 drives (mixing) shaft 19 rotatory, and (mixing) shaft 19 then drives stirring vane 20 and rotates, makes the mixture in the reaction vessel 2 rotate, improves the reaction.
Preferably, still include calcium magnesium concentration adjustment mechanism, calcium magnesium concentration adjustment mechanism includes calcium magnesium ion content detection mechanism, first breather pipe 21, second air pump 22 and carbon dioxide storage box 23, calcium magnesium ion content detection mechanism is arranged in detecting the calcium magnesium ion content of sewage in the former water tank of sewage 1, first breather pipe 21 one end stretches into in the former water tank of sewage 1, and the other end communicates with the end of giving vent to anger of second air pump 22, the inlet end and the inside intercommunication of carbon dioxide storage box 23 of second air pump 22.
The method comprises the steps that the calcium and magnesium concentration of sewage in a raw sewage tank 1 is required to be kept below a threshold value due to the fact that a low calcium and magnesium concentration environment needs to be maintained in a reaction barrel 2, the calcium and magnesium concentration of the sewage in the raw sewage tank 1 needs to be adjusted, firstly, the calcium and magnesium ion concentration of the sewage in the raw sewage tank 1 is detected through a calcium and magnesium ion content detection mechanism, if the calcium and magnesium concentration is higher than the set threshold value, carbon dioxide stored in a carbon dioxide storage tank 23 can be led into the raw sewage tank 1 through a first vent pipe 21 by a second air pump 22, the carbon dioxide can chemically react with calcium ions and magnesium ions in the sewage to generate solid calcium carbonate and magnesium carbonate, the calcium carbonate and the magnesium carbonate are precipitated at the bottom of the raw sewage tank 1, and the low calcium and magnesium concentration sewage can be obtained after the calcium and magnesium ions are taken out of the raw sewage tank 1.
Preferably, the calcium and magnesium ion content detection mechanism comprises a sampling box 24, a sampling pipe 25, a first three-way electromagnetic valve 26, a second three-way electromagnetic valve 27, a second three-way electromagnetic valve 28, a calcium and magnesium concentration controller 29, a weighing sensor 30 and a drainage mechanism, wherein the first three-way electromagnetic valve 26 is arranged on the water inlet pipe 4, one end of the sampling pipe 25 is connected with the first three-way electromagnetic valve 26, the other end of the sampling pipe is positioned above the sampling box 24, the second three-way electromagnetic valve 28 is connected with the second air pump 22, the first vent pipe 21 and the second vent pipe 27 are both connected with the second three-way electromagnetic valve 28, the second vent pipe 27 extends into the sampling box 24, the weighing sensor 30 is arranged on the inner bottom surface of the sampling box 24, the drainage mechanism comprises a drainage pipe 31, a drainage electromagnetic valve 32 and a filter screen 33, the drainage pipe 31 is connected with the bottom surface of the sampling box 24, the filter screen 33 is arranged on the inner bottom surface of the sampling box 24, and is located above the drain pipe 31, the drain electromagnetic valve 32 is arranged on the drain pipe 31, and the calcium and magnesium concentration controller 29 is arranged on the outer side wall of the sampling box 24 and is electrically connected with the first three-way electromagnetic valve 26, the second three-way electromagnetic valve 28, the second air pump 22, the weighing sensor 30 and the drain electromagnetic valve 32 respectively.
For the detection of the concentration of calcium and magnesium ions in the raw sewage tank 1, the detection is not directly performed in the raw sewage tank 1, but a sampling detection mode is adopted, during the detection, the calcium and magnesium concentration controller 29 controls the first three-way electromagnetic valve 26 to be switched to another channel, so that the front half part of the water inlet pipe 4 is communicated with the sampling pipe 25, the sewage in the raw sewage tank 1 can enter the sampling tank 24, the sewage capacity in the sampling tank 24 is controlled to be constant, then the calcium and magnesium concentration controller 29 controls the second three-way electromagnetic valve 28 to enable the second vent pipe 27 to be communicated with the second air pump 22, so that the second air pump 22 can introduce the carbon dioxide in the carbon dioxide storage tank 23 into the sampling tank 24 through the second vent pipe 27, in the process, calcium carbonate solids and magnesium carbonate solids are continuously generated in the sampling tank 24, the weighing sensor 30 can detect the total weight in the sampling tank 24 in real time, when the weight in the sampling box 24 does not change at a certain moment, it represents that all calcium ions and magnesium ions have reacted with carbon dioxide and reach a saturated state, then the calcium-magnesium concentration controller 29 controls the opening of the water discharge electromagnetic valve 32 to make the sewage in the sampling box 24 flow out from the water discharge pipe 31, after the sewage completely flows out, the weight collected by the weighing sensor 30 is the weight of calcium carbonate solid and magnesium carbonate solid, knowing the weight of calcium carbonate and magnesium carbonate in the sampling box 24, the initial capacity of the sewage in the sampling box 24 and the sewage capacity in the raw sewage tank 1, the calcium-magnesium ion concentration contained in the sewage in the raw sewage tank 1 can be obtained through proportional operation, if the calcium-magnesium ion concentration is higher, the calcium-magnesium concentration controller 29 controls the switching passage of the second electromagnetic valve to make the first vent pipe 21 communicate with the second air pump 22, so that the carbon dioxide in the carbon dioxide storage tank 23 can enter the raw sewage tank 1 through the first vent pipe 21, after the calcium and magnesium ions in the sewage raw water tank 1 react, the calcium and magnesium ions in the sewage are reduced, and the concentration of the calcium and magnesium ions in the sewage entering the reaction barrel 2 is ensured to be lower than a set threshold value.
The calcium and magnesium concentration adjusting mechanism further comprises a gas flow sensor 34, the gas flow sensor 34 is arranged inside the first vent pipe 21 and the second vent pipe 27, and the calcium and magnesium concentration controller 29 is electrically connected with the gas flow sensor 34.
In order to accurately grasp the amount of carbon dioxide introduced into the raw sewage tank 1, the gas flow sensors 34 are respectively arranged in the first vent pipe 21 and the second vent pipe 27, when the weight detected by the weighing sensor 30 is not changed, the sewage in the sampling tank 24 is in a saturated state, the total flow of carbon dioxide in the saturated state can be obtained through the gas flow sensors 34, the total amount of carbon dioxide to be introduced into the raw sewage tank 1 is calculated by the calcium-magnesium concentration controller 29 according to the calcium-magnesium concentration threshold value in the sewage, and the total amount of carbon dioxide to be introduced into the raw sewage tank 1 is monitored through the gas flow sensors 34.
Preferably, the device also comprises a precipitate removing mechanism, the precipitate removing mechanism is respectively arranged in the raw sewage tank 1 and the sampling box 24, the precipitate removing mechanism comprises a rotating motor 35, a pulling rope 36, a lifting plate 37 and a baffle plate 38, the lifting plate 37 is arranged at the bottom surfaces of the raw sewage tank 1 and the sampling box 24, the weighing sensor 30 is arranged inside the lifting plate 37, the rotating motor 35 is arranged at the top of the side walls of the raw sewage tank 1 and the sampling box 24, one end of the pulling rope 36 is wound on the output shaft of the rotating motor 35, the other end extends downwards to be connected with the top surface of the lifting plate 37, the baffle plate 38 is arranged above the inner side wall of the raw sewage tank 1 and above the inner side wall of the sampling box 24 and is positioned on the ascending path of the lifting plate 37, the calcium and magnesium concentration controller 29 is electrically connected with the rotating motor 35, the lifting plate 37 is provided with a plurality of through holes 39, the sediment removing mechanism further comprises an elevating platform 40, the elevating platform 40 is arranged on the tops of the side walls of the sewage raw water tank 1 and the sampling tank 24, and the rotating motor 35 is arranged on the top surface of the elevating platform 40.
When carbon dioxide is introduced into the raw sewage tank 1 or the sampling tank 24, calcium carbonate solids and magnesium carbonate solids are generated, and in order to facilitate the detection of the sampling tank 24 again and the flowing of sewage in the raw sewage tank 1, a precipitate removing mechanism is arranged in the invention (the raw sewage tank 1 and the sampling tank 24 are similar in structure and only have different sizes and different external pipeline connections, so that only a schematic connection structure between the sampling tank 24 and the precipitate removing mechanism is shown in fig. 2 of the invention), the calcium magnesium concentration controller 29 drives the rotating motor 35 to work, the rotating motor 35 can roll the pull rope 36 and drive the lifting plate 37 to ascend, because the lifting plate 37 is provided with the through hole 39, the sewage in the raw sewage tank 1 can pass through the through hole 39, the lifting plate 37 drives the calcium carbonate solids and the magnesium carbonate solids to move upwards, when the lifting plate 37 moves to the bottom of the baffle 38, one side of the lifting plate 37 is blocked by the baffle 38, and rotating electrical machines 35 continues to drive stay cord 36 rolling, because rotating electrical machines 35 sets up on adding high platform 40, consequently lifter plate 37 can take place the slope, and the calcium carbonate solid and the magnesium carbonate solid that are located on lifter plate 37 can follow lifter plate 37 and slide to the outside of sampling box 24 and the former water tank of sewage 1 through baffle 38 top surface, realize getting rid of the precipitate, then can carry out normal sewage transport after dropping lifter plate 37 to the bottom again.
In order to facilitate the lifting of the lifting plate 37, the first vent pipe 21 and the second vent pipe 27 are realized by corrugated pipes, when gas is not required to be introduced, the first vent pipe 21 and the second vent pipe 27 can be upwards contracted and are withdrawn from the raw sewage tank 1 and the sampling tank 24, and the lifting plate 27 can be ensured to normally lift.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The device for culturing the free-state high-abundance complete nitrifying bacteria is characterized by comprising a raw sewage tank, a membrane bioreactor, an aeration mechanism and an ammonia nitrogen adjusting mechanism, wherein the raw sewage tank is used for storing sewage with low calcium and magnesium concentration; the aeration mechanism comprises an aeration head and a first air pump, wherein the aeration head is arranged in the reaction barrel and is connected with the first air pump through an air inlet pipe; the ammonia nitrogen adjustment mechanism comprises an upper computer and an ammonia nitrogen sensor, the water inlet pipe is provided with a water inlet pump, the ammonia nitrogen sensor is arranged inside the reaction barrel, and the upper computer is respectively electrically connected with the ammonia nitrogen sensor and the water inlet pump.
2. The apparatus for culturing free high-abundance nitrobacteria according to claim 1, further comprising an aeration control mechanism, wherein the aeration control mechanism comprises a gas flow meter, a gas flow regulating valve, a dissolved oxygen sensor and a dissolved oxygen controller, the dissolved oxygen sensor is arranged inside the reaction barrel, the gas flow regulating valve and the gas flow meter are both arranged on the gas inlet pipe, the gas flow meter is arranged between the gas pump and the gas flow regulating valve, and the dissolved oxygen controller is electrically connected with the gas flow regulating valve and the dissolved oxygen sensor respectively.
3. The device for culturing the free-state high-abundance complete nitrifying bacteria according to claim 1, characterized by further comprising a switch valve, wherein the bottom of the raw sewage tank is provided with a vent pipe, and the switch valve is arranged on the vent pipe, the water inlet pipe and the water outlet pipe.
4. The device for culturing the free-state high-abundance complete nitrifying bacteria according to claim 1, further comprising an overflow pipe, wherein the overflow pipe is arranged on the side wall of the raw sewage tank and the side wall of the reaction barrel, and the overflow pipe on the side wall of the reaction barrel is positioned above the water outlet pipe.
5. The device for culturing the free-form high-abundance complete nitrifying bacteria according to claim 1, further comprising a stirring mechanism, wherein the stirring mechanism comprises a stirring motor, a stirring shaft and a stirring blade, an output shaft of the stirring motor is connected with the top end of the stirring shaft, the bottom end of the stirring shaft extends into the reaction barrel, and the stirring blade is arranged on the outer side wall of the stirring shaft.
6. The device for culturing the free-form high-abundance fully nitrifying bacteria according to claim 1, further comprising a calcium-magnesium concentration adjusting mechanism, wherein the calcium-magnesium concentration adjusting mechanism comprises a calcium-magnesium ion content detecting mechanism, a first air pipe, a second air pump and a carbon dioxide storage tank, the calcium-magnesium ion content detecting mechanism is used for detecting the calcium-magnesium ion content of the sewage in the raw sewage tank, one end of the first air pipe extends into the raw sewage tank, the other end of the first air pipe is communicated with the air outlet end of the second air pump, and the air inlet end of the second air pump is communicated with the interior of the carbon dioxide storage tank.
7. The apparatus according to claim 6, wherein the calcium-magnesium ion content detecting mechanism comprises a sampling box, a sampling tube, a first three-way solenoid valve, a second vent pipe, a second three-way solenoid valve, a calcium-magnesium concentration controller, a weighing sensor and a drainage mechanism, the first three-way solenoid valve is disposed on the water inlet pipe, one end of the sampling tube is connected to the first three-way solenoid valve, the other end of the sampling tube is disposed above the sampling box, the second three-way solenoid valve is connected to the second air pump, the first vent pipe and the second vent pipe are both connected to the second three-way solenoid valve, the second vent pipe extends into the sampling box, the weighing sensor is disposed on the inner bottom surface of the sampling box, the drainage mechanism comprises a drainage pipe, a drainage solenoid valve and a filter screen, the drainage pipe is connected to the bottom surface of the sampling box, the filter screen is arranged on the inner bottom surface of the sampling box and is positioned above the drain pipe, the drain electromagnetic valve is arranged on the drain pipe, and the calcium and magnesium concentration controller is arranged on the outer side wall of the sampling box and is respectively and electrically connected with the first three-way electromagnetic valve, the second air pump, the weighing sensor and the drain electromagnetic valve.
8. The apparatus for culturing high-abundance free nitrifying bacteria according to claim 7, wherein the calcium-magnesium concentration regulating mechanism further comprises a gas flow sensor, the gas flow sensor is disposed inside the first vent pipe and the second vent pipe, and the calcium-magnesium concentration controller is electrically connected to the gas flow sensor.
9. The apparatus according to claim 6, further comprising a precipitate removing mechanism, wherein the precipitate removing mechanism is disposed in the raw sewage tank and the sampling tank, respectively, the precipitate removing mechanism comprises a rotary motor, a pull rope, a lifting plate and a baffle plate, the lifting plate is disposed on the bottom surfaces of the raw sewage tank and the sampling tank, the weighing sensor is disposed inside the lifting plate, the rotary motor is disposed on the top of the raw sewage tank and the side wall of the sampling tank, one end of the pull rope is wound on the output shaft of the rotary motor, the other end of the pull rope extends downwards to be connected with the top surface of the lifting plate, the baffle plate is disposed above the inner side wall of the raw sewage tank and above the inner side wall of the sampling tank and is located on the ascending path of the lifting plate, and the calcium and magnesium concentration controller is electrically connected with the rotary motor, the lifting plate is provided with a plurality of through holes.
10. The apparatus according to claim 9, wherein the sediment removing mechanism further comprises an elevating platform, the elevating platform is arranged on the tops of the side walls of the raw sewage tank and the sampling tank, and the rotating motor is arranged on the top surface of the elevating platform.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110000851A1 (en) * | 2009-07-01 | 2011-01-06 | Vanotti Matias B | High Performance Nitrifying Sludge For High Ammonium Concentration and Low Temperature Wastewater Treatment |
| CN104611246A (en) * | 2013-11-05 | 2015-05-13 | 中国石油化工股份有限公司 | Synchronous cultivation method of nitrobacteria and aerobic denitrobacteria |
| CN106830302A (en) * | 2017-01-20 | 2017-06-13 | 浙江大学 | The MBR complete nitrifications bacterium concentrating device and its method of dissolved oxygen Automated condtrol |
| US20200223716A1 (en) * | 2018-02-01 | 2020-07-16 | University Of Central Florida Research Foundation, Inc. | Iron filings-based green environmental media for nutrient removal and methods of use |
| US20200346956A1 (en) * | 2019-05-01 | 2020-11-05 | University Of Manitoba | Dynamic process control for partial nitritation in wastewater treatment with autotrophic nitrogen removal |
| CN112299559A (en) * | 2020-09-07 | 2021-02-02 | 太原理工大学 | Whole-process nitrification, short-process denitrification and anaerobic ammonia oxidation single-stage coupling process control method |
-
2021
- 2021-08-27 CN CN202111000087.9A patent/CN113772803B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110000851A1 (en) * | 2009-07-01 | 2011-01-06 | Vanotti Matias B | High Performance Nitrifying Sludge For High Ammonium Concentration and Low Temperature Wastewater Treatment |
| CN104611246A (en) * | 2013-11-05 | 2015-05-13 | 中国石油化工股份有限公司 | Synchronous cultivation method of nitrobacteria and aerobic denitrobacteria |
| CN106830302A (en) * | 2017-01-20 | 2017-06-13 | 浙江大学 | The MBR complete nitrifications bacterium concentrating device and its method of dissolved oxygen Automated condtrol |
| US20200223716A1 (en) * | 2018-02-01 | 2020-07-16 | University Of Central Florida Research Foundation, Inc. | Iron filings-based green environmental media for nutrient removal and methods of use |
| US20200346956A1 (en) * | 2019-05-01 | 2020-11-05 | University Of Manitoba | Dynamic process control for partial nitritation in wastewater treatment with autotrophic nitrogen removal |
| CN112299559A (en) * | 2020-09-07 | 2021-02-02 | 太原理工大学 | Whole-process nitrification, short-process denitrification and anaerobic ammonia oxidation single-stage coupling process control method |
Non-Patent Citations (1)
| Title |
|---|
| 高瑶远等: "低溶解氧环境下全程硝化活性污泥的特性", 《中国环境科学》 * |
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