CN113759047A - Method for detecting saponin content in ginseng and method for identifying garden ginseng and wild ginseng - Google Patents
Method for detecting saponin content in ginseng and method for identifying garden ginseng and wild ginseng Download PDFInfo
- Publication number
- CN113759047A CN113759047A CN202111127338.XA CN202111127338A CN113759047A CN 113759047 A CN113759047 A CN 113759047A CN 202111127338 A CN202111127338 A CN 202111127338A CN 113759047 A CN113759047 A CN 113759047A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- wild
- content
- sample
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000208340 Araliaceae Species 0.000 title claims abstract description 235
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 235
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 235
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 235
- 238000000034 method Methods 0.000 title claims abstract description 56
- 229930182490 saponin Natural products 0.000 title claims abstract description 38
- 150000007949 saponins Chemical class 0.000 title claims abstract description 38
- 239000001397 quillaja saponaria molina bark Substances 0.000 title claims abstract description 30
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 239000011259 mixed solution Substances 0.000 claims abstract description 19
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000019253 formic acid Nutrition 0.000 claims abstract description 15
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 14
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000010828 elution Methods 0.000 claims abstract description 10
- 239000012488 sample solution Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 39
- 235000017709 saponins Nutrition 0.000 claims description 33
- 239000000843 powder Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 11
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract description 7
- YURJSTAIMNSZAE-HHNZYBFYSA-N ginsenoside Rg1 Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YURJSTAIMNSZAE-HHNZYBFYSA-N 0.000 description 12
- 238000011160 research Methods 0.000 description 8
- 238000007865 diluting Methods 0.000 description 7
- 239000003814 drug Substances 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- TXEWRVNOAJOINC-UHFFFAOYSA-N ginsenoside Rb2 Natural products CC(=CCCC(OC1OC(COC2OCC(O)C(O)C2O)C(O)C(O)C1O)C3CCC4(C)C3C(O)CC5C6(C)CCC(OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C(C)(C)C6CCC45C)C TXEWRVNOAJOINC-UHFFFAOYSA-N 0.000 description 6
- 238000007689 inspection Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000013558 reference substance Substances 0.000 description 5
- UFNDONGOJKNAES-UHFFFAOYSA-N Ginsenoside Rb1 Natural products CC(=CCCC(C)(OC1OC(COC2OC(CO)C(O)C(O)C2O)C(O)C(O)C1O)C3CCC4(C)C3C(O)CC5C6(C)CCC(OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C(C)(C)C6CC(O)C45C)C UFNDONGOJKNAES-UHFFFAOYSA-N 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 229930182494 ginsenoside Natural products 0.000 description 4
- GZYPWOGIYAIIPV-JBDTYSNRSA-N ginsenoside Rb1 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GZYPWOGIYAIIPV-JBDTYSNRSA-N 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003908 quality control method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- YURJSTAIMNSZAE-UHFFFAOYSA-N UNPD89172 Natural products C1CC(C2(CC(C3C(C)(C)C(O)CCC3(C)C2CC2O)OC3C(C(O)C(O)C(CO)O3)O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O YURJSTAIMNSZAE-UHFFFAOYSA-N 0.000 description 2
- 238000012850 discrimination method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229940089161 ginsenoside Drugs 0.000 description 2
- PFSIGTQOILYIIU-UHFFFAOYSA-N ginsenoside Rb3 Natural products CC(=CCCC(C)(O)C1CCC2(C)C3CCC4C(C)(C)C(CCC4(C)C3CC(OC5OC(COC6OCC(O)C(O)C6O)C(O)C(O)C5O)C12C)OC7OC(CO)C(O)C(O)C7OC8OC(CO)C(O)C(O)C8O)C PFSIGTQOILYIIU-UHFFFAOYSA-N 0.000 description 2
- PWAOOJDMFUQOKB-WCZZMFLVSA-N ginsenoside Re Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](O[C@@H]2[C@H]3C(C)(C)[C@@H](O)CC[C@]3(C)[C@@H]3[C@@]([C@@]4(CC[C@@H]([C@H]4[C@H](O)C3)[C@](C)(CCC=C(C)C)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C)(C)C2)O[C@H](CO)[C@@H](O)[C@@H]1O PWAOOJDMFUQOKB-WCZZMFLVSA-N 0.000 description 2
- CBEHEBUBNAGGKC-UHFFFAOYSA-N ginsenoside Rg1 Natural products CC(=CCCC(C)(OC1OC(CO)C(O)C(O)C1O)C2CCC3(C)C2C(O)CC4C5(C)CCC(O)C(C)(C)C5CC(OC6OC(CO)C(O)C(O)C6O)C34C)C CBEHEBUBNAGGKC-UHFFFAOYSA-N 0.000 description 2
- AOGZLQUEBLOQCI-UHFFFAOYSA-N ginsenoside-Re Natural products CC1OC(OCC2OC(OC3CC4(C)C(CC(O)C5C(CCC45C)C(C)(CCC=C(C)C)OC6OC(CO)C(O)C(O)C6O)C7(C)CCC(O)C(C)(C)C37)C(O)C(O)C2O)C(O)C(O)C1O AOGZLQUEBLOQCI-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- FBFMBWCLBGQEBU-RXMALORBSA-N (2s,3r,4s,5s,6r)-2-[(2r,3r,4s,5s,6r)-2-[[(3s,5r,6s,8r,9r,10r,12r,13r,14r,17s)-3,12-dihydroxy-4,4,8,10,14-pentamethyl-17-[(2s)-6-methyl-2-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhept-5-en-2-yl]-2,3,5,6,7,9,11,12,13,15,16,17-dodecah Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O FBFMBWCLBGQEBU-RXMALORBSA-N 0.000 description 1
- NFZYDZXHKFHPGA-UHFFFAOYSA-N 17alpha-hydroxygofruside Natural products C12CC(C)(C)CCC2(C(=O)OC2C(C(O)C(O)C(CO)O2)O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OC(C(O)=O)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O NFZYDZXHKFHPGA-UHFFFAOYSA-N 0.000 description 1
- FBFMBWCLBGQEBU-GYMUUCMZSA-N 20-gluco-ginsenoside-Rf Natural products O([C@](CC/C=C(\C)/C)(C)[C@@H]1[C@H]2[C@H](O)C[C@H]3[C@](C)([C@]2(C)CC1)C[C@H](O[C@@H]1[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O2)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@H]1C(C)(C)[C@@H](O)CC[C@]31C)[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 FBFMBWCLBGQEBU-GYMUUCMZSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- QXQFFGOMXYKNBA-UHFFFAOYSA-N Chikusetsusaponin V Natural products CC1(C)CCC2(CCC3C(=CCC4C3(C)CCC5C(C)(C)C(CCC45C)OC6OC(C(O)C(O)C6OC7OC(CO)C(O)C(O)C7O)C(=O)O)C2C1)C(=O)OC8OC(CO)C(O)C(O)C8O QXQFFGOMXYKNBA-UHFFFAOYSA-N 0.000 description 1
- NFZYDZXHKFHPGA-QQHDHSITSA-N Chikusetsusaponin-V Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@H]1O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@]1(CCC(C[C@H]14)(C)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C(O)=O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O NFZYDZXHKFHPGA-QQHDHSITSA-N 0.000 description 1
- LLPWNQMSUYAGQI-QBQUQATFSA-N Ginsenoside R1 Natural products O([C@](CC/C=C(\C)/C)(C)[C@@H]1[C@H]2[C@@H](O)C[C@H]3[C@](C)([C@]2(C)CC1)C[C@H](O[C@@H]1[C@H](O[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)CO2)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@H]1C(C)(C)[C@@H](O)CC[C@]31C)[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1 LLPWNQMSUYAGQI-QBQUQATFSA-N 0.000 description 1
- HYPFYJBWSTXDAS-UHFFFAOYSA-N Ginsenoside Rd Natural products CC(=CCCC(C)(OC1OC(CO)C(O)C(O)C1O)C2CCC3(C)C4CCC5C(C)(C)C(CCC5(C)C4CC(O)C23C)OC6OC(CO)C(O)C(O)C6OC7OC(CO)C(O)C(O)C7O)C HYPFYJBWSTXDAS-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- YQKCHRBAJSATCG-UHFFFAOYSA-N UNPD30744 Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC(C)(CCC=C(C)C)C2C3C(C4(CCC5C(C)(C)C(OC6C(C(O)C(O)C(CO)O6)OC6C(C(O)C(O)C(CO)O6)O)CCC5(C)C4CC3O)C)(C)CC2)O1 YQKCHRBAJSATCG-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000013524 data verification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- NODILNFGTFIURN-GZPRDHCNSA-N ginsenoside Rb2 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1OC[C@H](O)[C@H](O)[C@H]1O NODILNFGTFIURN-GZPRDHCNSA-N 0.000 description 1
- NODILNFGTFIURN-USYOXQFSSA-N ginsenoside Rb3 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O NODILNFGTFIURN-USYOXQFSSA-N 0.000 description 1
- RBRANZURTULKJD-UHFFFAOYSA-N ginsenoside Ro Natural products CC1(C)CCC2(CCC3(C)C(=CCC4C5(C)CCC(OC6OC(C)(C(O)C(O)C6OC7OC(CO)C(O)C(O)C7O)C(=O)O)C(C)(C)C5CCC34C)C2C1)C(=O)OC8OC(CO)C(O)C(O)C8O RBRANZURTULKJD-UHFFFAOYSA-N 0.000 description 1
- UVBLDLGZDSGCSN-UHFFFAOYSA-N ginsenoside-Rb3 Natural products C1=CC2C3(C)CCC(O)C(C)(C)C3CCC2(C)C2(C)CCC34CCC(C)C(C)C4C21OC3=O UVBLDLGZDSGCSN-UHFFFAOYSA-N 0.000 description 1
- ZTQSADJAYQOCDD-UHFFFAOYSA-N ginsenoside-Rd2 Natural products C1CC(C2(CCC3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC(C(C(O)C1O)O)OC1COC1OCC(O)C(O)C1O ZTQSADJAYQOCDD-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- LLPWNQMSUYAGQI-OOSPGMBYSA-N notoginsenoside R1 Chemical compound O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)CO2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O LLPWNQMSUYAGQI-OOSPGMBYSA-N 0.000 description 1
- JURZHOVRCOWZFN-UHFFFAOYSA-N notoginsenoside R1 Natural products CC(=CCCC(C)(OC1OC(CO)C(O)C(O)C1O)C2CCC3(C)C2C(O)CC4C5(C)CCC(O)C(C)(C)C5C(CC34C)OC6OC(COC7OCC(O)C(O)C7O)C(O)C(O)C6O)C JURZHOVRCOWZFN-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 1
- UOJAEODBOCLNBU-UHFFFAOYSA-N vinaginsenoside R4 Natural products C1CC(C2(CC(O)C3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O UOJAEODBOCLNBU-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for detecting the content of saponin in ginseng and a method for identifying garden ginseng and wild ginseng. The method for detecting the content of the saponin in the ginseng comprises the following steps: detecting the saponin content in the ginseng sample solution to be detected by adopting a high performance liquid chromatography; wherein the detection conditions include: gradient elution is carried out by adopting a mobile phase A and a mobile phase B; the mobile phase A is a mixed solution of formic acid and acetonitrile; the mobile phase B is a mixed solution of formic acid and water. Based on the detection method of the saponin content in the ginseng, a method for identifying garden ginseng and wild ginseng is established. The detection method has low requirement on instruments, more measurable components, high separation degree and short time consumption, and the method for identifying the garden ginseng and the wild ginseng is quick, efficient and accurate and has simple, convenient and clear standard parameters.
Description
Technical Field
The invention relates to a method for detecting the content of saponin in ginseng and a method for identifying garden ginseng and wild ginseng.
Background
Ginseng is a famous and precious medicinal material with the advantages of protecting liver, regulating immunity, resisting fatigue, resisting diabetes, resisting tumor, resisting depression and treating cardiovascular and cerebrovascular diseases and nervous system diseases. According to the growing environment and cultivation method of ginseng, it can be divided into 4 types of garden ginseng, wild ginseng and wild ginseng. The natural and originally ecological ginseng which is naturally spread and grows in the dense forest of the deep mountain is called wild ginseng. Wild ginseng is the best quality of ginseng, the yield is very low, resources are exhausted, and private collection, digging and selling are not allowed, so that ginseng cultivation and semi-cultivation ginseng are mainly used in the market, wherein the artificially-cultivated ginseng is commonly called as 'garden ginseng', the commodity years are more than 4-5 years, and generally the commodity years are not more than 6 years; the ginseng which is sowed in wild states of mountain forests and naturally grows is called as 'mountain ginseng under forest', is called as 'seed sea', and the commercial life is more than 10 years; according to the national standard regulation of 'wild ginseng identification and grading quality GB/T18765-2015', ginseng naturally growing in dense forest in deep mountain after sowing can be called wild ginseng, and the commercial life is more than 15 years and about 20 years. More and more researches prove that the wild ginseng has the inherent quality and medicinal value which are obviously superior to those of garden ginseng and are closer to those of the wild ginseng, the value is not so high, the price is often over ten thousand for each sale, the wholesale price of the garden ginseng is only hundreds of thousands of yuan per kilogram, and the price difference between the wild ginseng and the garden ginseng is very different, so the scientific identification of the wild ginseng and the garden ginseng has important significance.
At present, the discrimination of wild ginseng and garden ginseng is mainly based on empirical discrimination, and appearance characteristics of reed rhizome, , body, grain, beard, skin, point and the like are main discrimination points, but the method has extremely high requirements on the discrimination experience of an identifier, has different evaluation results from people, is too subjective and has no repeatability, and has almost no discrimination capability on powdery raw material samples of wild ginseng and garden ginseng.
In recent years, thanks to the rapid development of modern science and technology, technical methods related to ginseng discrimination are endless, and earlier literature arrangement finds that, besides the original appearance character discrimination of ginseng, the discrimination of wild ginseng and garden ginseng also comprises methods such as volume and quantity of microscopic features and xylem proportion inspection, comparison of infrared spectrum shape and intensity, fitting of liquid chromatogram fingerprint, molecular biological gene sequencing, analysis of ginsenoside content, metabonomics research based on analytical chemistry and the like. However, the method has certain limitations in the daily quality control work of identification of raw material medicines and finished product inspection, so that the conversion of the actual production and study results is difficult to realize, and particularly under the condition that the demand of ginseng market is increased year by year, a simpler and more convenient quality control index with better distinguishing property is urgently needed.
The 2015 edition of national standard, namely 'quality of identification and grading of wild ginseng', only stipulates that the content of ginsenoside Rb1 in wild ginseng or wild ginseng powder is more than 0.40%, the sum of the contents of ginsenoside Re and Rg1 is more than 0.60%, and the content of total saponins of ginseng is more than 4.40%, and the standard is consistent with the essence of the control standard of the content of garden ginseng and forest ginseng in the 'Chinese pharmacopoeia' (2020 edition), only carries out minimum content limitation on 3 main saponin components, and the situation that the garden ginseng and forest ginseng standards are commonly used together exists, so that people are difficult to effectively distinguish the garden ginseng and forest ginseng standards in practical application. See table 1 for details.
TABLE 1 content specification of ginseng standard for ginsenosides
Furthermore, the related results of the analytical chemistry-based metabonomics studies reported in the literature, such as Identification of mountain-free-concentrated and concentrated-concentrated using UPLC/oa-TOF MSE with a multi-concentrated specific sample-developing strategy (Doi: 10.1016/j.jgr.2015.11.001), research of chemical composition of Linnakai (Red sea forest, D. Jilin, 2017) based on NMR and UPLC-QTOF-MS/MS techniques, research of chemical composition of Linnakai (upright metabolism for the differentiation of the concentrated metabolism of the mountain-derived metabolites of PLC/UHQT-MS) (Doi: 10.1016/J.20132. of J.2019. of the national culture of mountain-concentrated growing theory of mountain-TOF 19. of mountain-concentrated growing theory of Chinese medicinal plants (mountain-TOF 19. of mountain-collected growth of mountain-TOF 19. and US) (mountain-1. J.11.11.21. of the same university of Chinese medicinal materials, mountain-TOF, US; mountain-D. J.11.11.11.11.11.11.19. and J.11.11.11.11.7. the same No. 7. of the same No. 7. Tokyo.7. of the same No. 7. Tokyo.12. A.12. A.A.A.A.A.A.A.11.A.A.D.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.D. 2. Identification of the plant of the Identification of the same No. of mountain-A.D.A.A.A.A.A.A.A.A.A.A.A.D.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.D.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.D.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A. The obtained differences and excessive specific metabolic markers exist in the northast China (Doi: 10.3390/molecules24010033), the analysis and detection method has higher requirements on instruments, and the defects of content standards and indexes are not clear, so the results probably have certain significance in scientific research, but the judgment indexes and the limited range in practical working scenes such as daily inspection and the like under the condition of large-scale production of medicines are not clearly indicated, so that the scientific research and the practical production application are in a non-negligible disjointed condition.
The patent "a method for identifying the type and growth period of ginseng by using the content and proportion of ginsenoside" (application number: 201811572380.0) clearly indicates that three ratios of Re/Rg1, Rb1/Rg1 and Rf/Rg1 can identify the type and growth period of a sample, and respectively specifies the content ranges of Rg, Rg 20 to 25 years, Rg 26 to 29 years, Rg1, Re/1, Rb 1/1 and Rf/1 in the 3-year or less garden ginseng, 3-5-year garden ginseng, 6-year or more garden ginseng, 8-to 12-year or more forest ginseng, 13-to 15-year or more forest ginseng, 16-year or more forest ginseng, 8-to 10-year or more wild ginseng, 11-to 14-year or more wild ginseng, 15-to 19-year or more wild ginseng, Rg 20 to 25-year or more wild ginseng, 26-to 29-year or more wild ginseng. However, there still exist many problems in the whole discrimination method, firstly, the patent says that the names of various ginseng are in doubt, the "wild ginseng" is supposed to be the "wild ginseng", the "wild ginseng" can be named as the "wild ginseng" in the forest for more than 15 years, and whether the "wild ginseng" is adopted or not is not clearly indicated; secondly, the discrimination indexes adopt Rg1, Re/Rg1, Rb1/Rg1 and Rf/Rg1 to carry out range limitation, the discrimination method is extremely complicated, the discrimination parameters are too many and the range is too narrow, the universality and the feasibility in the work of daily medicine inspection and the like are not high, and the method is not good in repeatability and low in accuracy because the ginseng is extremely easily influenced by the environment in the growing process.
In addition, in a patent of 'method for identifying wild ginseng participating in garden ginseng, wild ginseng under forest and garden ginseng' (application number CN202010868017.4), the content of ginsenoside Rb1 and the ratio of Rb1/Ro are considered for distinguishing the wild ginseng participating in garden ginseng, wild ginseng under forest and garden ginseng, and when the content of Rb1 accounts for more than or equal to 0.6% of the weight of a sample and the content of Rb1/Ro is more than or equal to 2.0, the sample is wild ginseng (wild ginseng under forest with the growth age of more than or equal to 15 years), but the garden ginseng can not meet the two conditions simultaneously; when Rb1/Ro is more than or equal to 2.0, more than 80% of samples of wild ginseng (5-14 years) meet the standard, and more than 90% of samples of garden ginseng do not meet the standard. In contrast, the discrimination rate of the mountain ginseng under forest (5-14 years) is low, and the detection method adopts an ultra-high performance liquid chromatograph for analysis, although the detection time is greatly shortened, the requirement on instruments is high, and the method universality is slightly poor.
Other literature methods which have low requirements on instruments, more measurable components and better separation degree inevitably have the defects of overlong detection time, high reagent cost and the like. For example, in the process of simultaneously detecting 9 saponin components, the reference takes 130min per sample injection to complete corresponding analysis, while in the Chinese pharmacopoeia (2020 edition) under the ginseng term and in the appendix A in the wild ginseng identification and grading quality (GB/T18765-2015), the analysis methods are consistent, and take 100min per sample injection, which seriously affects the daily inspection period, in HPLC method for determining the content of 9 ginsenosides in wild ginseng of different ages (ginseng research, 2020, 32 (04): 7-10).
Disclosure of Invention
The invention provides a method for detecting the content of saponin in ginseng and a method for identifying garden ginseng and wild ginseng, aiming at solving the defects of high requirement on instruments, less detection components, low separation degree, long time consumption, poor universality, low repeatability, difficulty in accurately identifying garden ginseng and wild ginseng and the like in the process of detecting ginseng samples in the prior art. The method for detecting the content of the saponin in the ginseng has low requirement on instruments, more measurable components, high separation degree and short time consumption, and the method for identifying the garden ginseng and the wild ginseng is quick, efficient and accurate and has simple, convenient and clear standard parameters.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a method for detecting the content of saponin in ginseng, which comprises the following steps: detecting the saponin content in the ginseng sample solution to be detected by adopting a high performance liquid chromatography; wherein,
the detection conditions of the high performance liquid chromatography comprise: gradient elution is carried out by adopting a mobile phase A and a mobile phase B; the mobile phase A is a mixed solution of formic acid and acetonitrile, wherein the formic acid accounts for 0.01-0.5% of the total volume of the mobile phase A; the mobile phase B is a mixed solution of formic acid and water, wherein the formic acid accounts for 0.01-0.5% of the total volume of the mobile phase B;
the saponin comprises one or more of R1, Rg1, Re, Rf, Rb1, Ro, Rb2, Rb3 and Rd.
In the present invention, preferably, the formic acid accounts for 0.1% of the total volume of the mobile phase a.
In the present invention, preferably, the formic acid accounts for 0.1% of the total volume of the mobile phase B.
In the present invention, the elution procedure of the gradient elution is preferably as shown in table 2:
TABLE 2 elution gradiometer
In the present invention, preferably, the detection conditions of the high performance liquid chromatography include: and (3) a C18 chromatographic column, wherein the flow rate is 1.0mL/min, the detection wavelength is 203nm, the column temperature is 30 ℃, and the sample injection amount is 1-10 mu L. Wherein the model of the C18 chromatographic column is preferably Agilent Poroshell 120EC-C18 (4.6X 150mm,2.7 μm).
In the present invention, the ginseng sample solution to be tested can be prepared by a method conventional in the art, and generally comprises: firstly, grinding a ginseng sample, then sieving the ginseng sample by a No. 2-8 sieve, drying the ginseng sample to constant weight, weighing the ginseng sample, adding the ginseng sample into a methanol-water mixed solution or an ethanol-water mixed solution, carrying out ultrasonic or reflux extraction, and filtering an extracting solution by a filter membrane to obtain the ginseng sample to-be-detected solution.
Wherein, preferably, the ginseng sample is sieved by a No. 4 sieve after being crushed.
Wherein the volume fraction of methanol in the methanol-water mixed solution can be 30-90%, for example 70%.
Wherein, the time of the ultrasonic treatment can be 10-120 min, preferably 100 min.
Wherein, the temperature of the ultrasonic wave can be 10-80 ℃, preferably 50 ℃.
Wherein, the pore diameter of the filter membrane is preferably 0.22 μm.
In the present invention, the high performance liquid chromatography may further include preparation of a control solution. The control solution may be prepared according to methods conventional in the art.
In the present invention, the high performance liquid chromatography may be performed using a liquid chromatograph that is conventional in the art, such as a high performance liquid chromatograph, an ultra high performance liquid chromatograph, or a high performance liquid chromatograph-mass spectrometer.
Based on the detection method of the saponin content in the ginseng, the invention simultaneously determines the content of 9 types of saponin in a plurality of batches of wild ginseng (wild ginseng original branch or wild ginseng powder) and garden ginseng samples, and researches the relationship and the rule between the main components through mathematical model analysis on the basis to establish the identification method of the garden ginseng and the wild ginseng.
The invention also provides a method for identifying the garden ginseng and the wild ginseng, which comprises the following steps: detecting the content of the saponins Rb1 and Ro in the ginseng sample, and calculating the content ratio of Rb1/Ro, so as to judge the ginseng type, wherein the judgment standard is as follows:
(1) when the content of Rb1 is more than or equal to 0.4 percent and the content of Rb1/Ro is more than or equal to 1.6, the ginseng sample is wild ginseng;
(2) when the content of Rb1 is more than or equal to 0.55%, the ginseng sample is wild ginseng;
(3) when Rb1/Ro is more than or equal to any value between 1.8 and 3.0, the ginseng sample is wild ginseng.
In the present invention, the method for detecting the content of saponins Rb1 and Ro can be conventional in the art, and is preferably the method for detecting the content of saponins in ginseng according to the present invention.
In the invention, the wild ginseng can be original wild ginseng or wild ginseng powder.
For the judgment standard (1), when the wild ginseng is wild ginseng powder, the judgment accuracy is 100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 94%, and only 6% of garden ginseng meets the judgment standard (1).
For the judgment standard (2), when the wild ginseng is wild ginseng powder, the judgment accuracy is 100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 89%, and only 6% of garden ginseng meets the judgment standard (2).
For the judgment standard (3), when the wild ginseng is wild ginseng powder, the judgment accuracy is 80-100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 83-89%, and only 6% of garden ginseng meets the judgment standard (2).
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows:
1. the high performance liquid chromatography which has low requirement on instruments, more measurable components, high separation degree and short time consumption is established, the contents of 9 saponin components (notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, Rf, ginsenoside Rb1, Ro, ginsenoside Rb2, ginsenoside Rb3 and ginsenoside Rd) in the ginseng can be simultaneously measured, and the method has the advantages of higher universality, higher accuracy and shorter chromatographic peak retention time (the peak can be completely obtained within 45min by adopting a common high performance liquid chromatograph).
2. According to the method, the content of Rb1 and the ratio of Rb1/Ro are compared, simple and clear standard parameters are given, wild mountain participation in garden ginseng can be judged quickly, efficiently and accurately, and the accuracy is high; meanwhile, the method has higher universality and feasibility in daily medicine inspection and other works, and is beneficial to quality control and product development of ginseng sold in the market.
Drawings
FIG. 1 is a chromatogram of a sample of example 1 of the present invention.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
Laboratory apparatus
An LC-20AD Shimadzu high performance liquid chromatograph equipped with a binary solvent system, an automatic sample injection manager, a column incubator, a UV detector and a Labsolutions chromatographic workstation; XS204 four-digit electronic balance (METTLER TOLEDO), XS205 five-digit electronic balance (METTLER TOLEDO); an ultrasonic cleaner (SK2200 BT); HWS-26 constant temperature water bath
Experimental reagents and materials
Methanol (analytical grade, chemical reagents of national drug group, ltd); formic acid (analytically pure, chemical reagents of national drug group, ltd.); acetonitrile (chromatographically pure, Merck); purified water; saponin standard (China institute for testing food and drug)
Example 1: detection of saponin content in ginseng
(1) Preparation of ginseng sample solution to be tested
Firstly, crushing a ginseng sample to be detected, sieving the ginseng sample by a No. 4 sieve, then precisely weighing 2g of the ginseng sample to be detected, precisely adding 20mL of 70% methanol-water mixed solution, sealing, carrying out ultrasonic treatment at the initial temperature of 50 ℃ for 100min, cooling to room temperature, shaking up, filtering, discarding primary filtrate, precisely weighing 10mL of subsequent filtrate, evaporating the subsequent filtrate in a water bath at 80-85 ℃, dissolving residues by 70% methanol-water mixed solution, transferring the residues into a 5mL volumetric flask, adding 70% methanol-water mixed solution for diluting to a scale, shaking up, filtering by a 0.22 mu m microporous membrane, and taking the subsequent filtrate to obtain the ginseng.
(2) Preparation of control solutions
Precisely weighing control saponins R1, Rg1, Re, Rf, Rb1, Ro, Rb2, Rb3 and Rd, respectively, placing in a 5mL volumetric flask, adding 70% methanol-water mixed solution, dissolving, diluting to scale mark, and mixing to obtain control stock solution. And taking a proper amount of the stock solution, adding 70% methanol-water mixed solution, gradually diluting for 2 times, and diluting for 5 times to obtain a series of mixed reference substance solutions.
(3) High performance liquid chromatography detection
Chromatographic column Agilent Poroshell 120EC-C18(4.6 × 150mm,2.7 μm), flow rate of 1.0mL/min, detection wavelength of 203nm, column temperature of 30 deg.C, sample injection amount of 10 μ L; gradient elution was performed using mobile phase a and mobile phase B, the gradient elution procedure is shown in table 2.
The content of saponin in wild ginseng powder, wild ginseng original branch and garden ginseng is respectively detected according to the method, and the chromatogram obtained by detection is shown in figure 1. Wherein A is wild ginseng powder, B is original branch of wild ginseng, and C is garden ginseng; 1-notoginsenoside R1, 2-ginsenoside Rg1, 3-ginsenoside Re, 4-ginsenoside Rf, 5-ginsenoside Rb1, 6-ginsenoside Ro, 7-ginsenoside Rb2, 8-ginsenoside Rb3, and 9-ginsenoside Rd.
In addition, the separation data of the control and the sample are shown in table 3.
TABLE 3 degree of separation of control and sample
Example 2: method for identifying garden ginseng and wild ginseng
The contents of Rb1 and Ro in the samples were measured by the detection method of example 1 using randomly selected 17 batches of Yuanshen, 18 batches of wild ginseng (original ginseng) and 10 batches of wild ginseng (powder), and the content ratio of Rb1/Ro was calculated, and the measured data are shown in Table 4 below.
Based on the detection method in example 1, the control solution was prepared as follows:
precisely weighing 50mg of ginsenoside Rb1 reference substance and 25mg of ginsenoside Ro reference substance, placing in the same 5mL volumetric flask, adding 70% methanol-water mixed solution, dissolving and diluting to scale mark, and mixing to obtain reference substance stock solution. And taking a proper amount of the stock solution, adding 70% methanol-water mixed solution, gradually diluting for 2 times, and diluting for 5 times to obtain a series of mixed reference substance solutions.
TABLE 4 Saponin content (%) and ratio (n. 2) in Ginseng radix samples
The above results were analyzed to obtain the judgment criteria of the garden ginseng and the wild ginseng, as shown in table 5. When the percentage content of Rb1 is more than or equal to 0.4 percent and Rb1/Ro is more than or equal to 1.6, 100 percent of samples of wild ginseng (powder) meet the parameter condition, 94 percent of samples of wild ginseng (original branch) meet the parameter condition, and only 6 percent of garden ginseng simultaneously meets the two conditions; when the percentage content of Rb1 is more than or equal to 0.55%, 100% of the wild ginseng (powder) sample meets the parameter condition, 89% of the wild ginseng (original branch) sample meets the parameter condition, and only 6% of the wild ginseng meets the condition; when Rb1/Ro is larger than or equal to any value between 1.8 and 3.0, 80 to 100 percent of samples of the wild ginseng (powder) meet the parameter condition, 83 to 89 percent of samples of the wild ginseng (original branch) meet the parameter condition, and only 6 percent of garden ginseng meets the condition.
TABLE 5 judgment standards of Yuanshen and wild ginseng
And then, the accuracy of the set parameters is verified through supplementary data verification and blind test experiments.
Claims (10)
1. A method for detecting the content of saponin in ginseng comprises the following steps: detecting the saponin content in the ginseng sample solution to be detected by adopting a high performance liquid chromatography; wherein,
the detection conditions of the high performance liquid chromatography comprise: gradient elution is carried out by adopting a mobile phase A and a mobile phase B; the mobile phase A is a mixed solution of formic acid and acetonitrile, wherein the formic acid accounts for 0.01-0.5% of the total volume of the mobile phase A; the mobile phase B is a mixed solution of formic acid and water, wherein the formic acid accounts for 0.01-0.5% of the total volume of the mobile phase B;
the saponin comprises one or more of R1, Rg1, Re, Rf, Rb1, Ro, Rb2, Rb3 and Rd.
2. The method for detecting the content of saponin in ginseng according to claim 1, wherein formic acid accounts for 0.1% of the total volume of the mobile phase A;
and/or the formic acid accounts for 0.1 percent of the total volume of the mobile phase B.
3. The method for detecting the content of the saponin in the ginseng according to claim 1, wherein the elution procedure of the gradient elution is as follows:
4. the method for detecting the content of saponin in ginseng according to claim 1, wherein the detection conditions of the high performance liquid chromatography comprise: and (3) a C18 chromatographic column, wherein the flow rate is 1.0mL/min, the detection wavelength is 203nm, the column temperature is 30 ℃, and the sample injection amount is 1-10 mu L.
5. The method for detecting the content of saponin in ginseng according to claim 1, wherein the preparation of the ginseng sample solution to be detected comprises: firstly, grinding a ginseng sample, then sieving the ginseng sample by a No. 2-8 sieve, drying the ginseng sample to constant weight, weighing the ginseng sample, adding the ginseng sample into a methanol-water mixed solution or an ethanol-water mixed solution, carrying out ultrasonic or reflux extraction, and filtering an extracting solution by a filter membrane to obtain the ginseng sample to-be-detected solution.
6. A method for detecting the content of saponin in ginseng according to claim 5, wherein the ginseng sample is ground and then screened through a No. 4 sieve;
and/or the volume fraction of methanol in the methanol-water mixed solution is 30-90%, such as 70%;
and/or the ultrasonic time is 10-120 min, preferably 100 min;
and/or the temperature of the ultrasonic wave is 10-80 ℃, preferably 50 ℃;
and/or the pore diameter of the filter membrane is 0.22 mu m.
7. The method for detecting the content of saponin in ginseng according to claim 1, wherein the high performance liquid chromatography further comprises the preparation of a control solution;
and/or the high performance liquid chromatography adopts a high performance liquid chromatograph, an ultra high performance liquid chromatograph or a high performance liquid chromatography-mass spectrometer.
8. A method for identifying garden ginseng and wild ginseng comprises the following steps: detecting the content of the saponins Rb1 and Ro in the ginseng sample, and calculating the content ratio of Rb1/Ro, so as to judge the ginseng type, wherein the judgment standard is as follows:
(1) when the content of Rb1 is more than or equal to 0.4 percent and the content of Rb1/Ro is more than or equal to 1.6, the ginseng sample is wild ginseng;
(2) when the content of Rb1 is more than or equal to 0.55%, the ginseng sample is wild ginseng;
(3) when Rb1/Ro is more than or equal to any value between 1.8 and 3.0, the ginseng sample is wild ginseng.
9. The method for discriminating a garden ginseng from a wild ginseng according to claim 8, wherein the method for detecting the contents of the saponins Rb1 and Ro is the method for detecting the contents of saponins in a ginseng according to any one of claims 1 to 7.
10. The method for identifying Yuanshen and Yeshanshen according to claim 8, wherein the Yeshanshen is a wild ginseng original or powder;
for the judgment standard (1), when the wild ginseng is wild ginseng powder, the judgment accuracy is 100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 94%, and only 6% of garden ginseng meets the judgment standard (1);
for the judgment standard (2), when the wild ginseng is wild ginseng powder, the judgment accuracy is 100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 89%, and only 6% of garden ginseng meets the judgment standard (2);
for the judgment standard (3), when the wild ginseng is wild ginseng powder, the judgment accuracy is 80-100%; when the wild ginseng is the original wild ginseng, the judgment accuracy rate is 83-89%, and only 6% of garden ginseng meets the judgment standard (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111127338.XA CN113759047B (en) | 2021-09-18 | 2021-09-18 | Method for detecting content of saponins in ginseng and method for identifying garden ginseng and wild ginseng |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111127338.XA CN113759047B (en) | 2021-09-18 | 2021-09-18 | Method for detecting content of saponins in ginseng and method for identifying garden ginseng and wild ginseng |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113759047A true CN113759047A (en) | 2021-12-07 |
| CN113759047B CN113759047B (en) | 2023-06-20 |
Family
ID=78797541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111127338.XA Active CN113759047B (en) | 2021-09-18 | 2021-09-18 | Method for detecting content of saponins in ginseng and method for identifying garden ginseng and wild ginseng |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113759047B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116124954A (en) * | 2023-02-27 | 2023-05-16 | 上海市质量监督检验技术研究院 | Rapid detection method for ginsenoside Rg1, re and Rb1 content in ginseng |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991330A (en) * | 2019-04-04 | 2019-07-09 | 上海上药杏灵科技药业股份有限公司 | A kind of detection method of the finger-print of ginseng under forest |
| CN111912927A (en) * | 2020-08-26 | 2020-11-10 | 沈阳药科大学 | Method for identifying wild ginseng and garden ginseng |
| CN112798724A (en) * | 2020-12-18 | 2021-05-14 | 云南金碧制药有限公司 | Method for establishing saponin component chromatographic fingerprint suitable for ginseng traditional Chinese medicinal materials and medicinal material extracts |
-
2021
- 2021-09-18 CN CN202111127338.XA patent/CN113759047B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109991330A (en) * | 2019-04-04 | 2019-07-09 | 上海上药杏灵科技药业股份有限公司 | A kind of detection method of the finger-print of ginseng under forest |
| CN111912927A (en) * | 2020-08-26 | 2020-11-10 | 沈阳药科大学 | Method for identifying wild ginseng and garden ginseng |
| CN112798724A (en) * | 2020-12-18 | 2021-05-14 | 云南金碧制药有限公司 | Method for establishing saponin component chromatographic fingerprint suitable for ginseng traditional Chinese medicinal materials and medicinal material extracts |
Non-Patent Citations (5)
| Title |
|---|
| LIANLIAN ZHU ET AL: "The distinct of chemical profiles of mountainous forest cultivated ginseng and" * |
| 严华 等: "基于特征图谱的人参属药材西洋参、人参、 三七的比较" * |
| 孙海等: "郑培 和;,利用HPLC/MS 对野 山参和林下参皂苷的分 析" * |
| 朱莹莹等: "HPLC 同时 测定人参药性菌质中8 种皂苷含量" * |
| 黄赵刚 等: "RP-HPLC法测定三七中11 种皂苷的含量" * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116124954A (en) * | 2023-02-27 | 2023-05-16 | 上海市质量监督检验技术研究院 | Rapid detection method for ginsenoside Rg1, re and Rb1 content in ginseng |
| CN116124954B (en) * | 2023-02-27 | 2024-06-07 | 上海市质量监督检验技术研究院 | Rapid detection method for ginsenoside Rg1, re and Rb1 content in ginseng |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113759047B (en) | 2023-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111323509B (en) | Plant sex identification method based on metabolome global analysis | |
| CN107356691B (en) | Method for detecting fingerprint of Jianqu | |
| CN106932517A (en) | A kind of analysis method for differentiating Mel Jujubae and the adulterated Mel Jujubae of syrup | |
| CN115060822B (en) | Fingerprint spectrum quantitative analysis method based on traditional Chinese medicine 'imprinting template' component clusters | |
| CN113759047B (en) | Method for detecting content of saponins in ginseng and method for identifying garden ginseng and wild ginseng | |
| CN113189253A (en) | Method for detecting nanoscale plastic particles in soil environment | |
| CN111912927A (en) | Method for identifying wild ginseng and garden ginseng | |
| CN107576739B (en) | HPLC fingerprint detection method of Longmu Zhuanggu granules | |
| CN106872616B (en) | Method for distinguishing rhizoma paridis major and rhizoma paridis Yunnanensis | |
| CN108663440A (en) | Callicarpa nudiflora medicinal material UPLC fingerprint map constructions method and standard finger-print | |
| CN111220719A (en) | Method for evaluating quality of ginseng medicinal material by using fingerprint spectrum | |
| CN109696513A (en) | A method of identifying ginseng type and its growth year using ginsenoside component content and its ratio | |
| CN114034798B (en) | Red water dendrobium stem flower fingerprint construction and content determination method | |
| CN112415104B (en) | Characteristic spectrum, construction method and detection method of pyrrosia pedunculata medicinal material, decoction pieces, standard decoction and formula granules thereof | |
| CN112666302B (en) | Method for identifying active flavone component group in barley seedling and rapidly detecting active flavone component group | |
| CN114088837A (en) | Linseed oil adulteration detection method based on triglyceride fingerprint spectrum and application thereof | |
| CN109507334A (en) | The detection method of amino acid content and the grade separation method of tabasheer in tabasheer | |
| CN111610271A (en) | Identification method of authentic rhizoma paridis | |
| CN115453032B (en) | Method for detecting authenticity and content of torreya seed oil based on peach phenol characteristics | |
| CN110954615A (en) | Plant sex identification method by using characteristic metabolites | |
| CN109142595A (en) | A kind of preparation method of paralytic shellfish poisoning (PSP) standard solution | |
| CN115436552B (en) | Gas chromatography-mass spectrometry method for determining authenticity and content of torreya seed oil based on content of characteristic components | |
| CN112345653B (en) | Method for detecting natural rubber in rubberella by thermal cracking gas chromatography-mass spectrometry | |
| CN113624871B (en) | High performance liquid chromatography determination method for residual amount of hexachlorocyclotriphosphazene in grain | |
| CN115616111A (en) | Method for simultaneously detecting content of 12 phenolic acids in citrus fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |