CN113707924A - Microbial fuel cell and construction method and application thereof - Google Patents
Microbial fuel cell and construction method and application thereof Download PDFInfo
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Abstract
The invention provides a microbial fuel cell and a construction method and application thereof. The invention adopts the coupling of solid state fermentation and fuel cell, can generate electricity while fermenting and producing chemicals, and realizes the co-production of chemicals and electricity; the solid adsorption carrier is adopted, so that the solid adsorption carrier can be repeatedly used, the situation that the electronic carrier is consumed is avoided, the stress resistance of microorganisms can be improved, the density of microbial cells is improved, and the yield of fermentation products is improved; the microbial fuel cell derived from the sugar source and metabolic activity of biomass can be kept stable in each batch or continuous fermentation, and further the stability of output electric energy is guaranteed.
Description
Technical Field
The invention belongs to the field of microbial electrochemistry, relates to a microbial fuel cell, and particularly relates to a microbial fuel cell and a construction method and application thereof.
Background
The biological fermentation industry is an important civil industry and plays an important role in the biological manufacturing industry. The biofermentation industry converts organic matter into high value-added products mainly by microorganisms. The product relates to organic acids, alcohols, amino acids, enzyme preparations, vitamins, antibiotics, drugs and other chemicals. In recent years, a new microbial anaerobic energy metabolism pattern, extracellular respiration, has been discovered. Microorganisms with this extracellular respiration can produce electrons in metabolic activity that are transferred to a solid to form an electric current. This means that microorganisms can not only produce high value-added chemicals but also generate electricity during fermentation, which changes the knowledge that people have been single fermented products. However, this part of energy is not effectively utilized in the conventional fermentation industry, resulting in waste of resources. The microbial fuel cell is a novel green power generation mode, and is a device for directly converting chemical energy in organic matters into electric energy by using microbes.
CN 112390374A discloses a method for improving the electricity generation and nitrogen and phosphorus removal performance of a microalgae cathode microbial fuel cell, which improves the electricity generation performance and increases the adsorption quantity of nitrogen and phosphorus pollutants by immobilizing microalgae. However, the nutrient content in the sewage is complex and the difference between different batches is large, so that the fluctuation of the electric energy output is large and the electric energy output is difficult to utilize. In addition, the problems of low electron transfer rate, easy leakage, poor safety and the like exist in the liquid matrix, and the application of the coupling fermentation of the microbial fuel cell is limited to a great extent.
CN 105647981A discloses a method for strengthening the utilization of glycerol by microbial thallus through an electrochemical system and application thereof, comprising the steps of strain activation, seed culture and anaerobic fermentation, wherein the anaerobic fermentation adopts electrochemical fermentation, an electronic carrier is added in a fermentation culture medium, and the electronic carrier is a compound with the characteristic of redox couple; CN 108103136A discloses a method for producing succinic acid by strengthening microbial cells through an electrochemical system and application thereof, and the method comprises the steps of strain activation, seed culture and anaerobic fermentation, wherein the anaerobic fermentation adopts electrochemical fermentation, an electronic carrier is added into a fermentation culture medium, and the concentration of the electronic carrier is 0.1-1.0 mmol/L; the electronic carrier disclosed above is consumed continuously in the power generation process, needs to be replenished at regular time, and cannot be reused.
Based on the above research, how to provide a microbial fuel cell, wherein microbial carrier can effectively improve extracellular electron transfer rate, improves the stress resistance of microorganism, and then improves thallus density, and can not be consumed, can used repeatedly, microbial fuel cell can realize generating electricity when producing chemicals in fermentation, realizes the coproduction of chemicals and electric energy, has become the problem that needs to be solved at present urgently.
Disclosure of Invention
The invention aims to provide a microbial fuel cell and a construction method and application thereof, wherein a microbial carrier in the microbial fuel cell can interact with microorganisms, the extracellular electron transfer rate is effectively improved, the stress resistance of the microorganisms is improved, the density of thalli is further improved, the thalli cannot be consumed, the microbial fuel cell can be repeatedly used, the microbial fuel cell can realize power generation while producing chemicals through fermentation, and the co-production of chemicals and electric energy is realized.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a microbial fuel cell, which comprises an anode chamber, wherein a solid state fermentation system is filled in the anode chamber, and the solid state fermentation system comprises a solid adsorption carrier and microbes.
The solid adsorption carrier is used as a microbial carrier, and can interact with microbes to improve the stress resistance of the microbes, further improve the density of microbial cells and increase the yield of fermentation products; meanwhile, the solid adsorption carrier can effectively improve the transfer rate of electrons outside the cells, is not consumed and can be repeatedly used; in addition, the fuel cell is coupled with the solid state fermentation, so that the power generation can be realized while the chemicals are produced by fermentation, and the co-production of the chemicals and the electric energy is realized.
Preferably, the anode chamber further comprises an anode electrode and a culture medium.
Preferably, the solid adsorbent support is immersed in the culture medium.
Preferably, the solid adsorbent support comprises a combination of a carbon felt and a sponge, a combination of a carbon felt and a gauze, or a combination of a carbon felt and a non-woven fabric.
Preferably, the microorganism is an electroactive microorganism.
Preferably, the electroactive microorganism comprises any one or a combination of at least two of clostridium acetobutylicum, yeast, aeromonas hydrophila, thiobacillus or bacillus subtilis, and typical but non-limiting combinations include a combination of clostridium acetobutylicum and yeast, a combination of clostridium acetobutylicum and aeromonas hydrophila, or a combination of thiobacillus and bacillus subtilis.
Preferably, the microbial fuel cell further comprises a cathode compartment, a cathode electrode, a catholyte and a proton exchange membrane.
Preferably, the anode electrode and the cathode electrode are each independently a carbon felt.
Preferably, the catholyte comprises 20 to 30mM potassium ferricyanide in water, for example 20mM, 25mM or 30mM, but not limited to the values recited, and other values not recited in the range of values are equally applicable.
In a second aspect, the present invention provides a method of constructing a microbial fuel cell according to the first aspect, the method comprising:
and placing the seed solution, the culture medium, the anode electrode, the cathode electrode, the solid adsorption carrier and the proton exchange membrane of the microorganism in a fuel cell reactor, and sterilizing to obtain the microbial fuel cell.
Preferably, the seed solution of the microorganism is obtained by culturing the microorganism in corn meal solution.
Preferably, the volume ratio of the seed liquid to the culture medium of the microorganism is 1 (9-11), for example, 1:9, 1:10 or 1:11, but not limited to the enumerated values, and other unrecited values in the numerical range are also applicable.
Preferably, the solid adsorption carrier is a pretreated adsorption carrier, and the pretreatment method comprises soaking the solid adsorption carrier in an acid solution, washing, and drying.
Preferably, the acid solution is 0.8-1.2 mol/L hydrochloric acid, for example, 0.8mol/L, 1mol/L or 1.2mol/L, but not limited to the values listed, and other values not listed in the numerical range are also applicable.
Preferably, the manner of flushing comprises flushing with water to neutrality.
In a third aspect, the invention provides a use of a microbial fuel cell according to the first aspect, including in the field of co-production for electricity production and fermentation.
Compared with the prior art, the invention has the following beneficial effects:
the microbial fuel cell provided by the invention can generate electricity while producing chemicals by fermentation by adopting the coupling of solid state fermentation and the fuel cell, thereby realizing the co-production of chemicals and electricity; the solid adsorption carrier is adopted, so that the solid adsorption carrier can be repeatedly used, the situation that the electronic carrier is consumed is avoided, the stress resistance of microorganisms can be improved, the density of microbial cells is improved, and the yield of fermentation products is improved; the microbial fuel cell derived from the sugar source and metabolic activity of biomass can be kept stable in each batch or continuous fermentation, and further the stability of output electric energy is guaranteed.
Drawings
Fig. 1 is a schematic structural diagram of a microbial fuel cell provided by the present invention.
Fig. 2 is a graph of the real-time output voltage of the microbial fuel cells provided in example 4, example 5, example 6, and comparative example 1.
FIG. 3 shows the amounts of fermentation products obtained after 288 hours of culturing the microbial fuel cells provided in example 4, example 5, example 6 and comparative example 1.
The device comprises a solid adsorption carrier 1, an anode chamber 2, a cathode chamber 3, a proton exchange membrane 4, a data collector 5, an anode electrode 6 and a cathode electrode 7.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
The method for preparing a seed solution of a microorganism in the following embodiments comprises:
inoculating microorganisms into the corn flour solution, thermally shocking for 10 minutes at 100 ℃, and then placing the corn flour solution in an incubator at 37 ℃ for culturing for 48 hours to obtain a seed solution of the microorganisms; the inoculation amount of the inoculation accounts for 10wt% of the corn flour liquid, the inoculation times are 3 times, and the corn flour in the corn flour liquid accounts for 6 wt%.
The components of the culture medium used in the following embodiments include: 60g/L of C6H12O63.68g/L of (NH)4)2SO41.768g/L KH2PO42.938g/L of K2HPO42g/L of Ca (OH)26g/L yeast extract powder, 3g/L peptone and 10mL/L trace elements. The composition of the trace elements comprises 2.4g/L of Na2MoO41.5g/L of CaCl227g/L FeCl31g/L of CuSO40.29g/L of ZnSO41.7g/L of MnSO4·H2O, 12g/L MgSO4·7H2O, 1g/L of p-aminobenzoic acid and 1g/L of biotin.
The above description of the preparation method and the components of the culture medium of the microorganism seed solution is for the purpose of more fully illustrating the technical scheme, and should not be construed as a specific limitation of the present invention.
Example 1
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, 40 carbon felt and sponge combined adsorption carriers;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a sponge adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking the combined adsorption carrier in 1mol/L hydrochloric acid for 24 hours and washing the combined adsorption carrier with water to be neutral; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 25mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of carbon felt and sponge, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
The carbon felt anode electrode and the carbon felt cathode electrode of the microbial fuel cell are connected by a 10k omega resistance wire, two ends of the resistance wire are connected with a data acquisition unit, then the microbial fuel cell is cultured for 72 hours under the condition of 37 ℃ water bath, and the real-time output voltage and the amount of fermentation products are tested.
Example 2
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, 40 carbon felt and gauze combined adsorption carriers;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a gauze adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking the combined adsorption carrier in 1.2mol/L hydrochloric acid for 24 hours and washing the combined adsorption carrier with water to be neutral; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 20mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of a carbon felt and gauze, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
The carbon felt anode electrode and the carbon felt cathode electrode of the microbial fuel cell are connected by a 10k omega resistance wire, two ends of the resistance wire are connected with a data acquisition unit, then the microbial fuel cell is cultured for 72 hours under the condition of 37 ℃ water bath, and the real-time output voltage and the amount of fermentation products are tested.
Example 3
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, and a combined adsorption carrier of 40 carbon felts and non-woven fabrics;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a non-woven fabric adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking for 24 hours by using 0.8mol/L hydrochloric acid and washing to be neutral by using water; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 35mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of carbon felt and non-woven fabric, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
The carbon felt anode electrode and the carbon felt cathode electrode of the microbial fuel cell are connected by a 10k omega resistance wire, two ends of the resistance wire are connected with a data acquisition unit, then the microbial fuel cell is cultured for 72 hours under the condition of 37 ℃ water bath, and the real-time output voltage and the amount of fermentation products are tested.
Example 4
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, 40 carbon felt and sponge combined adsorption carriers;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a sponge adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking the combined adsorption carrier in 1mol/L hydrochloric acid for 24 hours and washing the combined adsorption carrier with water to be neutral; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 25mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of carbon felt and sponge, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
Connecting a carbon felt anode electrode and a carbon felt cathode electrode of the microbial fuel cell by using a 10k omega resistance wire, connecting two ends of the resistance wire with a data collector, and then culturing for 288h under a 37 ℃ water bath condition, wherein when culturing for 72h, 120h, 168h and 216h, a culture medium is replaced, and real-time output voltage and fermentation product amount are tested; the real-time output voltage curve of the microbial fuel cell is shown in fig. 2, and the amount of the fermentation product obtained after 288h of culture is shown in fig. 3.
Example 5
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, 40 carbon felt and gauze combined adsorption carriers;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a gauze adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking the combined adsorption carrier in 1.2mol/L hydrochloric acid for 24 hours and washing the combined adsorption carrier with water to be neutral; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 20mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of a carbon felt and gauze, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
Connecting a carbon felt anode electrode and a carbon felt cathode electrode of the microbial fuel cell by using a 10k omega resistance wire, connecting two ends of the resistance wire with a data collector, and then culturing for 288h under a 37 ℃ water bath condition, wherein when culturing for 72h, 120h, 168h and 216h, a culture medium is replaced, and real-time output voltage and fermentation product amount are tested; the real-time output voltage curve of the microbial fuel cell is shown in fig. 2, and the amount of the fermentation product obtained after 288h of culture is shown in fig. 3.
Example 6
This example provides a microbial fuel cell as shown in figure 1 comprising an anode compartment, a cathode compartment and a proton exchange membrane (dupont N-17);
the anode chamber comprises 20mL of seed solution of clostridium acetobutylicum, 200mL of culture medium, a carbon felt anode electrode, and a combined adsorption carrier of 40 carbon felts and non-woven fabrics;
the combined adsorption carrier is formed by combining a carbon felt adsorption carrier with the diameter of 20 multiplied by 3mm and a non-woven fabric adsorption carrier with the diameter of 20 multiplied by 3mm through a clip; the combined adsorption carrier is obtained by soaking for 24 hours by using 0.8mol/L hydrochloric acid and washing to be neutral by using water; the size of the carbon felt anode electrode is 50 multiplied by 100 multiplied by 3 mm;
the cathode chamber comprises 200mL of 35mM potassium ferricyanide aqueous solution and a carbon felt cathode electrode, and the size of the carbon felt cathode electrode is 50 multiplied by 100 multiplied by 3 mM;
the construction method of the microbial fuel cell comprises the following steps:
assembling seed solution of clostridium acetobutylicum, a culture medium, a carbon felt anode electrode, a carbon felt cathode electrode, a combined adsorption carrier of carbon felt and non-woven fabric, potassium ferricyanide catholyte and a proton exchange membrane (DuPont N-17) into a microbial fuel cell shown in figure 1, and sterilizing at 115 ℃ for 30 minutes to obtain the microbial fuel cell.
Connecting a carbon felt anode electrode and a carbon felt cathode electrode of the microbial fuel cell by using a 10k omega resistance wire, connecting two ends of the resistance wire with a data collector, and then culturing for 288h under a 37 ℃ water bath condition, wherein when culturing for 72h, 120h, 168h and 216h, a culture medium is replaced, and real-time output voltage and fermentation product amount are tested; the real-time output voltage curve of the microbial fuel cell is shown in fig. 2, and the amount of the fermentation product obtained after 288h of culture is shown in fig. 3.
Comparative example 1
This comparative example provides a microbial fuel cell which was the same as in example 1 except that the microbial fuel cell did not include a carbon felt adsorption carrier;
the construction method of the microbial fuel cell is the same as that of example 1;
connecting a carbon felt anode electrode and a carbon felt cathode electrode of the microbial fuel cell by using a 10k omega resistance wire, connecting two ends of the resistance wire with a data collector, and then culturing for 288h under a 37 ℃ water bath condition, wherein when culturing for 72h, 120h, 168h and 216h, a culture medium is replaced, and real-time output voltage and fermentation product amount are tested; the real-time output voltage curve of the microbial fuel cell is shown in fig. 2, and the amount of the fermentation product obtained after 288h of culture is shown in fig. 3.
Comparative example 2
This comparative example provides a microbial fuel cell that was the same as in example 1 except that the anode chamber included no carbon felt adsorption carrier, including 1mM neutral red electron carrier;
the microbial fuel cell was constructed in the same manner as in example 1.
The carbon felt anode electrode and the carbon felt cathode electrode of the microbial fuel cell are connected by a 10k omega resistance wire, two ends of the resistance wire are connected with a data acquisition unit, then the microbial fuel cell is cultured for 72 hours under the condition of 37 ℃ water bath, and the real-time output voltage and the amount of fermentation products are tested.
The culture output voltage and the amount of each fermentation product of the microbial fuel cell provided in the above examples and comparative examples are shown in table 1:
TABLE 1
From table 1, the following points can be seen:
(1) as can be seen from examples 1 and 4, the microbial fuel cell provided in example 4 can be cultured in a water bath at 37 ℃ for 288h, which can stabilize the output voltage and produce the fermentation product; similarly, as can be seen from examples 2 and 5, and examples 3 and 6, the microbial fuel cells provided in examples 5 and 6 can stabilize output voltage and yield fermentation products after being cultured in 37 ℃ water bath for 288 hours; therefore, the microbial fuel cell provided by the invention can stabilize the output voltage and produce fermentation products for a long time.
(2) As can be seen from example 1 and comparative example 1, the anode chamber of comparative example 1 is not provided with a solid adsorption carrier, and compared with example 1, the microbial fuel cell provided in comparative example 1 has reduced electricity generation and fermentation productivity; therefore, the solid adsorption carrier is used as a microorganism carrier, and can interact with microorganisms, so that the extracellular electron transfer rate can be effectively improved, the stress resistance of the microorganisms is improved, the density of microorganism thallus is further improved, and the yield of fermentation products is improved.
(3) As can be seen from example 1 and comparative example 2, comparative example 2 employs an electron carrier, which is continuously consumed during the fermentation process, and compared to example 1, the microbial fuel cell provided in comparative example 2 has reduced electricity generation and fermentation productivity; therefore, the solid adsorption carrier is used as the microbial carrier, so that the microbial carrier cannot be consumed in the fermentation process, can interact with the microbes, can effectively improve the extracellular electron transfer rate, improves the stress resistance of the microbes, further improves the density of microbial cells, and improves the yield of fermentation products.
In summary, the invention provides a microbial fuel cell and a construction method and application thereof, wherein the microbial fuel cell comprises an anode chamber, and a solid state fermentation system comprising a solid adsorption carrier and microbes is adopted in the anode chamber. The microbial fuel cell provided by the invention can generate electricity while producing chemicals by fermentation by adopting the coupling of solid state fermentation and the fuel cell, thereby realizing the co-production of chemicals and electricity; the solid adsorption carrier is adopted, so that the solid adsorption carrier can be repeatedly used, the situation that the electronic carrier is consumed is avoided, the stress resistance of microorganisms can be improved, the density of microbial cells is improved, and the yield of fermentation products is improved; the microbial fuel cell derived from the sugar source and metabolic activity of biomass can be kept stable in each batch or continuous fermentation, and further the stability of output electric energy is guaranteed.
The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.
Claims (10)
1. The microbial fuel cell is characterized by comprising an anode chamber, wherein a solid state fermentation system is filled in the anode chamber and comprises a solid adsorption carrier and microbes.
2. The microbial fuel cell of claim 1, wherein the anode chamber further comprises an anode electrode and a culture medium.
3. The microbial fuel cell of claim 1 or 2, wherein the solid adsorbent support comprises a combination of a carbon felt and a sponge, a combination of a carbon felt and a gauze, or a combination of a carbon felt and a non-woven fabric.
4. The microbial fuel cell of claim 3, wherein the microbes are electrically active microbes.
5. The microbial fuel cell of claim 4, wherein the electroactive microorganism comprises any one or a combination of at least two of Clostridium acetobutylicum, yeast, Aeromonas hydrophila, Thiobacillus, or Bacillus subtilis.
6. The microbial fuel cell of claim 5, further comprising a cathode compartment, a cathode electrode, a catholyte, and a proton exchange membrane.
7. A method of constructing a microbial fuel cell according to any one of claims 1 to 6, comprising:
and placing the seed solution, the culture medium, the anode electrode, the cathode electrode, the solid adsorption carrier and the proton exchange membrane of the microorganism in a fuel cell reactor, and sterilizing to obtain the microbial fuel cell.
8. The method according to claim 7, wherein the seed solution of the microorganism is obtained by culturing the microorganism in corn meal solution.
9. The construction method according to claim 8, wherein the solid adsorption carrier is a pretreated adsorption carrier, and the pretreatment method comprises soaking the solid adsorption carrier in acid solution, washing, and drying.
10. Use of a microbial fuel cell according to any of claims 1 to 6, comprising a co-production field for electricity production and fermentation.
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