CN113699079A - Bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof - Google Patents

Bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof Download PDF

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CN113699079A
CN113699079A CN202111091903.1A CN202111091903A CN113699079A CN 113699079 A CN113699079 A CN 113699079A CN 202111091903 A CN202111091903 A CN 202111091903A CN 113699079 A CN113699079 A CN 113699079A
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lyh5
diarrhea
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罗玉衡
兰聪
刘洋
申于青
李华
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Sichuan Agricultural University
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Abstract

The invention belongs to the technical field of biology, and discloses a bacteroides thetaiotaomicron LYH5 with the functions of relieving diarrhea and promoting growth, wherein the preservation numbers are as follows: CCTCC NO: M2021752; the preservation date is as follows: 23/6/2021, depository: china center for type culture Collection. The bacteroides thetaiotaomicron LYH5 can significantly improve the weight loss, average daily gain and average daily feed intake of diarrhea piglets, and significantly reduce the diarrhea rate of piglets. Although the diarrhea rate of the strain-treated piglets is still higher compared with that of the control group, the weight gain and the feed intake of the piglets are recovered to normal levels. Meanwhile, the invention also discloses the application of the Bacteroides thetaiotaomicron LYH 5.

Description

Bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof
Technical Field
The invention relates to the technical field of biology, in particular to a bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof.
Background
CN202011306542.3 discloses a Bacteroides thetaiotaomicron GL-02 and a complex microbial inoculum compounded with bile acid and application thereof. The classification name of the Bacteroides thetaiotaomicron is Bacteroides thetaiotaomicron, the Bacteroides thetaiotaomicron is preserved in the common microorganism center of the China Committee for culture Collection of microorganisms, and the preservation number is CGMCC No. 20818; the bacterial colony of the strain is round or similar to round, yellow, 0.5-2.5mm in diameter, smooth and lusterless in surface, slightly convex in middle, opaque and tidy in edge. The bacteroides thetaiotaomicron GL-02 has the effects of promoting nutrient absorption and improving the health of dairy cows, and the complex microbial inoculum compounded with bile acid can obviously improve the milk yield of the dairy cows and promote the growth of calves of the dairy cows.
The Bacteroides thetaiotaomicron GL-02 was isolated from the feces of healthy pigs.
CN202010814097.5 discloses the use of Bacteroides thetaiotaomicron (Bacteroides thetaiotaomicron) deposited in the chinese type culture collection at 2020, 4-month and 30-month with the deposit number CCTCC M2020105 in the preparation of food or medicine for preventing, alleviating or treating tropomyosin allergy in food. The Bacteroides thetaiotaomicron can regulate and control the imbalance of Th1/Th2 and Th17/Treg cell subsets, thereby relieving anaphylactic reaction caused by tropomyosin in food, relieving immune diseases such as food allergy, bronchial asthma and the like, and having strong pertinence, high economic benefit, green, environment-friendly and pollution-free raw materials and less side effect.
The Bacteroides thetaiotaomicron with the preservation number of CCTCC M2020105 is separated from a stool sample of a healthy population.
The technical problem to be solved by the scheme is as follows: the intestinal health of animals is improved and the growth is promoted by discovering a new bacteroides thetaiotaomicron strain.
Disclosure of Invention
The invention aims to provide a bacteroides thetaiotaomicron LYH5 with effects of relieving diarrhea and promoting growth, wherein the bacteroides thetaiotaomicron LYH5 can obviously improve the terminal weight, average daily gain and average daily feed intake of diarrhea piglets, and simultaneously obviously reduce the diarrhea rate of the piglets. Although the diarrhea rate of the strain-treated piglets is still higher compared with that of the control group, the weight gain and the feed intake of the piglets are recovered to normal levels.
Meanwhile, the invention also discloses the application of the Bacteroides thetaiotaomicron LYH 5.
In order to achieve the purpose, the invention provides the following technical scheme: a bacteroides thetaiotaomicron LYH5 with diarrhea alleviating and growth promoting effects has a deposit number of: CCTCC NO: M2021752; the preservation date is as follows: 23/6/2021, depository: china center for type culture Collection.
The Latin name is: bacteriodes theotiotropiron LYH 5;
the storage locations are: wuhan, Wuhan university.
Among the aforementioned Bacteroides thetaiotaomicron LYH5 having diarrhea-alleviating and growth-promoting effects, Bacteroides thetaiotaomicron LYH5 was isolated from fresh stool samples of healthy piglets.
In the Bacteroides thetaiotaomicron LYH5 with diarrhea-relieving and growth-promoting effects, the 16S r RNA gene sequence of Bacteroides thetaiotaomicron LYH5 is shown as SEQ ID NO: 1 is shown.
Meanwhile, the invention also discloses the application of the Bacteroides thetaiotaomicron LYH5 as an animal feed additive.
In the application of the Bacteroides thetaiotaomicron LYH5, the Bacteroides thetaiotaomicron LYH5 is used as a pig feed additive.
Compared with the prior art, the invention has the beneficial effects that:
the bacteroides thetaiotaomicron LYH5 is separated from the feces of healthy piglets, can obviously improve the end weight, average daily gain and average daily feed intake of diarrhea piglets, and simultaneously obviously reduces the diarrhea rate of the piglets. Although the diarrhea rate of the strain-treated piglets is still higher compared with that of the control group, the weight gain and the feed intake of the piglets are recovered to normal levels.
It can be used as feed additive for various animals, especially for mammals, especially pig, and has effects in relieving diarrhea and promoting growth.
Drawings
FIG. 1 shows the morphology and goblet cell distribution of the small intestine of piglets in the experimental group and the control group of example 1 of the present invention;
FIG. 2 is a comparison of the stool morphology of piglets in the experimental group and the control group of example 1 of the present invention;
FIG. 3 is a comparison of pig diarrhea rates in experimental and control groups in a large-scale farm according to example 1 of the present invention;
FIG. 4 is a comparison of pig diarrhea rates in experimental and control groups in a large-scale farm according to example 1 of the present invention;
FIG. 5 is a collection of Bacteroides thetaiotaomicron LYH5 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
Screening and identification of bacterial strains
1.1 isolation and purification of the Strain
Anaerobically collecting fresh feces sample of healthy piglet (DLY commercial pig), diluting with sterilized normal saline, filtering with sterilized gauze, and collecting dilution gradient of 10-8And 10-9The suspension liquid is respectively coated on a brain heart infusion Broth (BHI) agarose plate (agarose concentration is 15g/L liquid culture medium), after anaerobic culture is carried out for 36h under the condition of 37 ℃, single colony is selected to be streaked and purified on the BHI agarose plate for 3 times, then the single colony is selected to be enriched in the BHI liquid culture medium for 24h, and glycerol (the final concentration is 25 percent) is added into the culture medium for preservation at the temperature of minus 80 ℃.
(1) Identification of strains
The morphological characteristics, physiological and biochemical characteristics of the preserved strains are identified by referring to Bergey' S Manual of systematic bacteriology, and the genetic classification and identification are carried out by determining 16S r RNA gene sequences.
The strain morphological characteristics are as follows:
the strain is gram-negative bacillus and does not produce spores. Bacterial colonies on the BHI culture medium are gray-white semi-transparent circles, slightly convex in the middle, wet in surface and neat in edge.
Physiological and biochemical characteristics of the strain:
can produce glycoside hydrolase and polysaccharide lyase, can hydrolyze amylose, amylopectin, cellulose, inulin, pectin, and mannooligosaccharide, and can utilize glucose, sucrose, maltose, xylose, mannose, fructose, and arabinose, but cannot utilize lactose.
The 16S r RNA gene sequence of the strain is as follows (specifically, refer to SEQ ID NO: 1):
ataaaacattcacggactaccatgcaagtcgaggggcagcatttcagtttgcttgcaaactggagatggcgaccggcgcacgggtg agtaacacgtatccaacctgccgataactcggggatagcctttcgaaagaaagattaatacccgatggtataattagaccgcatggtcttgtta ttaaagaatttcggttatcgatggggatgcgttccattaggcagttggtgaggtaacggctcaccaaaccttcgatggataggggttctgaga ggaaggtcccccacattggaactgagacacggtccaaactcctacgggaggcagcagtgaggaatattggtcaatgggcgcaggcctga accagccaagtagcgtgaaggatgactgccctatgggttgtaaacttcttttatatgggaataaagttttccacgtgtggaattttgtatgtacca tatgaataaggatcggctaactccgtgccagcagccgcggtaatacggaggatccgagcgttatccggatttattgggtttaaagggagcgt aggtggacagttaagtcagttgtgaaagtttgcggctcaaccgtaaaattgcagttgatactggctgtcttgagtacagtagaggtgggcgga attcgtggtgtagcggtgaaatgcttagatatcacgaagaactccgattgcgaaggcagctcactggactgcaactgacactgatgctcgaa agtgtgggtatcaaacaggattagataccctggtagtccacacagtaaacgatgaatactcgctgtttgcgatatacagtaagcggccaagc gaaagcattaagtattccacctggggagtacgccggcaacggtgaaactcaaaggaattgacgggggcccgcacaagcggaggaacat gtggtttaattcgatgatacgcgaggaaccttacccgggcttaaattgcatttgaatatattggaaacagtatagccgtaaggcaaatgtgaag gtgctgcatggttgtcgtcagctcgtgccgtgaggtgtcggcttaagtgccataacgagcgcaacccttatctttagttactaacaggtcatgc tgaggactctagagagactgccgtcgtaagatgtgaggaaggtggggatgacgtcaaatcagcacggcccttacgtccggggctacacac gtgttacaatggggggtacagaaggcagctacctggtgacaggatgctaatcccaaaagcctctctcagttcggatcgaagtctgcaaccc gacttcgtgaagctggattcgctagtaatcgcgcatcagccatggcgcggtgaatacgttcccgggccttgtacacaccgcccgtcaagcc atgaaagccgggggtacctgaagtacgtaaccgcaaggagctcctaggtaaagcaaattgtttta
the similarity of the 16Sr RNA sequence of the strain and the 16Sr RNA sequence of Bacteroides thetaiotaomicron strain JCM 5827 in NCBI GenBank database is 99.79 percent, which indicates that the strain is Bacteroides thetaiotaomicron and is named as Bacteroides thetaiotaomicron LYH 5.
Second, verify the alleviating effect of Bacteroides thetaiotaomicron LYH5 on piglet diarrhea
(1) The research aims are as follows: by establishing a weaned piglet diarrhea model, whether Bacteroides thetiotamomicron LYH5 has the effects of relieving piglet diarrhea and improving the intestinal tract morphological structure of piglets so as to promote the growth of the piglets is examined.
(2) And (3) experimental design: 90 healthy 21-day-old weaned piglets with similar body conditions are selected and randomly divided into 3 groups (30 piglets/group, single-cage feeding) according to the principle of no weight difference. Normal feeding of control group piglets; the diarrhea group piglets adopt an environmental stress method to construct a piglet diarrhea model, and the specific operations are as follows: collecting feces of each piglet in the control group, uniformly mixing the feces, and uniformly distributing the feces to each piglet in the diarrhea group, wherein the temperature of the colony house is reduced by 5 ℃ on the normal basis; the strain treated piglets are subjected to environmental stress and gastric lavage of bacteriodes theonotioomicron LYH5 viable bacteria (viable bacteria number is 1 × 10)9CFU/mL). The control group and the diarrhea group were both perfused with the same volume of sterile medium. All piglets received the same diet. The test period is 28 days, all piglets are slaughtered after the test is finished, and duodenum, jejunum and ileum are collected and stored by 4% paraformaldehyde solution. Piglet feed intake was recorded daily, and the feces of each piglet was observed and scored to calculate diarrhea rate, and the piglets were weighed at the beginning and end of the trial.
(3) The material and the method are as follows:
strain medium composition (per 1000 ml): glucose 5g, peptone 10.0g, 10.0g yeast extract powder, NaHCO30.4g、Na2HPO4·12H2O 4.0g、K2HPO4 0.04g、KH2PO4 0.04g、MgSO4·7H2O 0.008g、 CaCl2·2H2O 0.008g、NaCl 0.08g、MnCl2·4H2O 0.01g、CoCl2·6H2O 0.001g、FeCl3·6H20.008 g of O, and adding oxygen-free distilled water to 1000mL, wherein 0.1% of resazurin is used as an anaerobic indicator.
Activating and culturing the strain:
taking out Bacteroides thetaiotaomicron LYH5 stored at-80 deg.C, inoculating into the above culture medium in anaerobic incubator at an inoculation ratio of 1:10, sealing anaerobic tube with butyl rubber plug and aluminum cover, culturing at 37 deg.C for 36-48 h to obtain bacterial solution OD600The culture was terminated at 0.8-1.0.
(4) Results and analysis:
as shown in the attached figure 1, the small intestine structure of the diarrhea piglets is seriously damaged and the number of goblet cells is obviously reduced compared with the control group, but the live bacteria of the lavage bacteria thetaiotaomicron LYH5 can obviously improve the small intestine villus structure of the piglets, and the number of the small intestine mucosa goblet cells of the piglets is equivalent to that of the control group, and the duodenum is especially obvious (the majority of the goblet cells are positioned at the bottom part of the intestinal villus, and the dark small dots are shown in the figure).
Meanwhile, compared with diarrhea piglets, the strain treatment can significantly improve the stool form of the piglets (figure 2).
TABLE 1 attached pigs growth performance and diarrhea rate
Figure BDA0003267658570000061
Note: the different letter superscripts in the same column indicate significant differences (P < 0.05).
As can be seen from the attached Table 1, the diarrhea piglets had significantly decreased weight loss, average daily gain and average daily feed intake (P <0.05) compared to the control group.
Compared with untreated diarrhea piglets, the live bacteria of the gastric lavage bacteria theonotioomicron LYH5 can obviously improve the end weight, average daily gain and average daily feed intake of the diarrhea piglets and obviously reduce the diarrhea rate of the piglets (P < 0.05). Although the diarrhea rate of the strain-treated piglets is still higher (P <0.05) compared with the control group, the weight gain and the feed intake of the piglets are restored to normal levels (P > 0.05).
Therefore, when the piglets have diarrhea, the live bacteria of the gastric lavage bacteria theotiotamicron LYH5 can help to repair the intestinal injury and greatly improve the growth performance of the piglets.
Thirdly, improvement effect of Bacteroides thetaiotaomicron LYH5 on piglet diarrhea in large-scale pig farm
(1) The purpose of the test is as follows: a large group of piglets in a large-scale farm with a low feeding management level is taken as a research object, and whether the strain can reduce the diarrhea rate of the piglets in the normal breeding process is examined by adding bacteria of the thiotaomicron LYH5 into the feed.
(2) And (3) experimental design: the test is carried out in a large-scale pig farm with relatively low feeding and management level in a certain place in the mountainous area of Yunnan. Randomly selecting 40 circles (about 400) of weaned piglets of the same pig, wherein 20 circles of piglets are subjected to strain treatment (strain freeze-dried powder is added into feed and added during feeding), and the rest are control groups. All piglets were fed normally and their stool morphology was observed daily and scored.
(3) The material and the method are as follows: anaerobic expanded culture was carried out on Bacteroides thetaiotaomicron LYH5 in a 50L liquid fermentor, with the medium formulation and culture conditions as above. Bacterial liquid OD (origin-destination) detection sample after 48 hours600And (4) stopping culturing after the strain is qualified, freeze-drying the bacterial liquid, and determining the number of the strains of the freeze-dried powder per unit weight according to a growth curve. The final concentration of the added strain in the feed is 1 × 109CFU/kg。
(4) And (3) test results: the addition of Bacteroides thetaiotaomicron LYH5 strain freeze-dried powder into the feed can obviously reduce the diarrhea rate of large groups of piglets (figure 3, P is less than 0.05), the reduction amplitude can reach 2.4 percent, and the strain has larger production and application potential.
Fourthly, the effect of Bacteroides thetaiotaomicron LYH5 compared with the effect of the same strain applied to weaned pigs
(1) The purpose of the test is as follows: by respectively adding Bacteroides thetaiotaomicron LYH5 and commercial strains into the feed of weaned piglets, the feed has more application advantages in improving the growth performance and diarrhea rate of the piglets compared with the conventional feed.
(2) And (3) experimental design: 60 healthy weaned piglets of 28 days old are selected, randomly divided into 3 groups according to the weight non-difference principle, and fed in a single cage. All piglets were fed the same commercial diet with free intake and drinking, with a test period of 28 days. Control piglets were not treated at all; adding Bacteroides thetaiotaomicron LYH5 lyophilized powder into BY group piglet feed; commercial strain Bacteroides thetaiotaomicron BNCC139203 (model strain, isolated from human feces and purchased from Beinanbiol) lyophilized powder was added to the piglet diet of BB group. The recovery and enrichment of the strain are carried out according to the method. Piglets were weighed at the beginning and end of the trial, and piglet feed intake and fecal scores were recorded daily.
(3) Results and analysis: as shown in attached table 2, the end weight and average daily gain of the weaned piglets of the BY group were significantly increased (P ═ 0.01) while significantly reducing the piglet feed-to-weight ratio and diarrhea rate (P <0.05) as compared to the control group and the BB group. Although the addition of the commercial strain Bacteroides thetaiotaomicron BNCC139203 lyophilized powder can also improve the growth performance of piglets by a small extent compared with the control group, the statistical difference is not achieved. These results demonstrate that bacteriodes theonotioomicron LYH5 isolated from the intestinal tract of piglets has advantages in application over the existing commercial strains of human origin, especially in terms of improving piglet growth performance and intestinal health.
TABLE 2 Effect of two Bacteroides thetaiotaomicron strains on piglet growth and diarrhea rates
Figure BDA0003267658570000071
Figure BDA0003267658570000081
Note: adding Bacteroides thetaiotaomicron LYH5 lyophilized powder into BY group piglet feed; bacteroides thetaiotaomicron BNCC139203 freeze-dried powder is added into the piglet feed of BB group. The different letter superscripts of the same row data indicate significant differences (P < 0.05).
Fifthly, the effects of bacteria theotiotamomicron LYH5 and the similar strains separated from the adult pig manure are compared when the strains are applied to the weaned piglets.
(1) The research aims are as follows: by establishing a weaned pig diarrhea model, compared with the fact that Bacteroides thetaiotaomicron LYH5 or the like bacteria separated from adult pig manure are added into a weaned pig feed, the model has more application advantages in the aspect of improving piglet diarrhea compared with the former.
(2) And (3) experimental design: referring to the separation and culture method in the scheme, a strain of like bacteria (LYHC1) is separated from fresh excrement of DLY fat pigs with the weight of about 130kg, and the strain is subjected to revival and enrichment culture by adopting the method in the scheme. Selecting 32 DLY healthy weaned piglets of 21 days old, and randomly dividing into 4 groups (8 piglets/group, single cage feeding) according to the principle of no weight difference: control group, diarrhea + LYH5, and diarrhea + LYHC 1. The method is characterized in that Bacteroides thetaiotaomicron LYH5 and LYHC1 freeze-dried powder are respectively added into the piglet feeds of all test pigs fed with standard commercial diets, diarrhea + LYH5 and diarrhea + LYHC1 groups, and the freeze-drying and adding modes of the strains refer to the method in the scheme. After the piglet is pre-fed for 3 days and adapted to the environment, diarrhea group piglets, diarrhea + LYH5 piglets and diarrhea + LYHC1 piglets adopt the method to establish a stress diarrhea model. All piglets had free food and water intake, with a test period of 21 days.
(3) Results and analysis: as shown in figure 4, when piglets suffer from stress diarrhea, the diarrhea rate (P <0.05) can be remarkably reduced by adding Bacteroides thetaiotaomicron LYH5 or the lyophilized powder of the same LYHC1 strain derived from the feces of fat pigs into the feed; however, compared with healthy piglets of a control group, the addition of the LYHC1 strain lyophilized powder can not effectively prevent diarrhea of piglets (P <0.05), and the addition of the bacteriodes theonotaomicron LYH5 lyophilized powder can effectively prevent diarrhea of piglets (P > 0.05). Therefore, when piglets are stressed, the control effect of Bacteroides thetaiotaomicron LYH5 on diarrhea is obviously better than that of similar strains separated from the feces of fat pigs, and the reason is probably that the Bacteroides thetaiotaomicron LYH5 is from the intestinal tracts of the piglets and has better piglet intestinal adaptability than the similar strains from the intestinal tracts of the fat pigs. Meanwhile, the test result also emphasizes the strain specificity of Bacteroides thetaiotaomicron application effect.
The experimental results can be seen in FIG. 4. The different letter superscripts in figure 4 indicate significant differences (P < 0.05). The control group is healthy piglets; the diarrhea group is a stress diarrhea model group; the diarrhea and LYH5 are the diarrhea piglet feed added with the strain freeze-dried powder; the diarrhea + LYHC1 is obtained by adding pig-derived homologous strain lyophilized powder into diarrhea piglet feed.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Sequence listing
<110> Sichuan university of agriculture
<120> a bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof
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ataaaacatt cacggactac catgcaagtc gaggggcagc atttcagttt gcttgcaaac 60
tggagatggc gaccggcgca cgggtgagta acacgtatcc aacctgccga taactcgggg 120
atagcctttc gaaagaaaga ttaatacccg atggtataat tagaccgcat ggtcttgtta 180
ttaaagaatt tcggttatcg atggggatgc gttccattag gcagttggtg aggtaacggc 240
tcaccaaacc ttcgatggat aggggttctg agaggaaggt cccccacatt ggaactgaga 300
cacggtccaa actcctacgg gaggcagcag tgaggaatat tggtcaatgg gcgcaggcct 360
gaaccagcca agtagcgtga aggatgactg ccctatgggt tgtaaacttc ttttatatgg 420
gaataaagtt ttccacgtgt ggaattttgt atgtaccata tgaataagga tcggctaact 480
ccgtgccagc agccgcggta atacggagga tccgagcgtt atccggattt attgggttta 540
aagggagcgt aggtggacag ttaagtcagt tgtgaaagtt tgcggctcaa ccgtaaaatt 600
gcagttgata ctggctgtct tgagtacagt agaggtgggc ggaattcgtg gtgtagcggt 660
gaaatgctta gatatcacga agaactccga ttgcgaaggc agctcactgg actgcaactg 720
acactgatgc tcgaaagtgt gggtatcaaa caggattaga taccctggta gtccacacag 780
taaacgatga atactcgctg tttgcgatat acagtaagcg gccaagcgaa agcattaagt 840
attccacctg gggagtacgc cggcaacggt gaaactcaaa ggaattgacg ggggcccgca 900
caagcggagg aacatgtggt ttaattcgat gatacgcgag gaaccttacc cgggcttaaa 960
ttgcatttga atatattgga aacagtatag ccgtaaggca aatgtgaagg tgctgcatgg 1020
ttgtcgtcag ctcgtgccgt gaggtgtcgg cttaagtgcc ataacgagcg caacccttat 1080
ctttagttac taacaggtca tgctgaggac tctagagaga ctgccgtcgt aagatgtgag 1140
gaaggtgggg atgacgtcaa atcagcacgg cccttacgtc cggggctaca cacgtgttac 1200
aatggggggt acagaaggca gctacctggt gacaggatgc taatcccaaa agcctctctc 1260
agttcggatc gaagtctgca acccgacttc gtgaagctgg attcgctagt aatcgcgcat 1320
cagccatggc gcggtgaata cgttcccggg ccttgtacac accgcccgtc aagccatgaa 1380
agccgggggt acctgaagta cgtaaccgca aggagctcct aggtaaagca aattgtttta 1440

Claims (5)

1. A bacteroides thetaiotaomicron LYH5 with diarrhea alleviating and growth promoting effects is characterized in that the preservation number is as follows: CCTCC NO: M2021752; the preservation date is as follows: 23/6/2021, depository: china center for type culture Collection.
2. The Bacteroides thetaiotaomicron LYH5 with the effects of alleviating diarrhea and promoting growth according to claim 1, wherein the Bacteroides thetaiotaomicron LYH5 is isolated from fresh stool samples of healthy piglets.
3. The Bacteroides thetaiotaomicron LYH5 with the effects of alleviating diarrhea and promoting growth according to claim 1, wherein the 16Sr RNA gene sequence of Bacteroides thetaiotaomicron LYH5 is as set forth in SEQ ID NO: 1 is shown.
4. Use of a bacteroides thetaiotaomicron LYH5 as defined in any one of claims 1-3 as an animal feed additive.
5. Use of Bacteroides thetaiotaomicron LYH5 as defined in claim 4 as a pig feed additive.
CN202111091903.1A 2021-09-17 2021-09-17 Bacteroides thetaiotaomicron LYH5 with diarrhea relieving and growth promoting effects and application thereof Active CN113699079B (en)

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