CN113697822B - Boron quantum dot and preparation method and application thereof - Google Patents
Boron quantum dot and preparation method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B35/00—Boron; Compounds thereof
- C01B35/02—Boron; Borides
- C01B35/023—Boron
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
- A61K41/0052—Thermotherapy; Hyperthermia; Magnetic induction; Induction heating therapy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/22—Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
- A61K49/222—Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations characterised by a special physical form, e.g. emulsions, liposomes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/63—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing boron
Abstract
The invention discloses a boron quantum dot and a preparation method and application thereof. The invention firstly provides a preparation method of boron quantum dots, which comprises the following steps: dispersing boron powder in water to obtain a boron nanosheet dispersion liquid; carrying out ultrasonic treatment on the boron nanosheet dispersion in an ice salt bath to obtain a mixed solution; and centrifuging the mixed solution, taking supernatant, and drying to obtain the boron quantum dots. The invention further provides the boron quantum dot prepared by the preparation method and application thereof. The boron quantum dots have good photo-thermal treatment effect and can perform photo-acoustic imaging at the same time, so that the integration of cancer diagnosis and treatment is realized. Meanwhile, the boron quantum dot can be effectively absorbed by tumor cells, so that the toxicity is low, and the treatment effect of cancer is good. In addition, the preparation method of the boron quantum dots has the advantages of simple process, low equipment requirement, low energy consumption and environmental friendliness, and can realize large-scale quantitative production.
Description
Technical Field
The invention relates to the technical field of preparation of photo-thermal reagents. More particularly, relates to a boron quantum dot and a preparation method and application thereof.
Background
Cancer is one of the major diseases threatening human health. How to effectively treat cancer is a hot spot of research all the time, but the current traditional treatment methods such as surgical excision, radiotherapy, chemotherapy and the like have the problems of large wound, easy relapse, large side effect and the like. As a novel cancer treatment method, photothermal therapy has attracted much attention due to its low toxic and side effects, low invasiveness, and excellent therapeutic effects, and photothermal agents, which are key materials in photothermal therapy, have been the focus of research (cn201910371146. X). In the research of photothermal reagents, various photothermal reagents are synthesized, and quantum dots with good photostability, strong broadband absorption and low cost are attracting attention. Ratio of<xnotran> (Sun, Z.; xie, H.; tang, S.; yu, X.F.; guo, Z.; shao, J.; zhang, H.; huang, H.; wang, H.; chu, P.K.Ultrasmall Black Phosphorus Quantum Dots: synthesis and Use as Photothermal Agents.Angew.Chem.Int.Ed.2015,54,11526-11530;CN201611207924.4), (Tao, W.; ji, X.; xu, X.; islam, M.A.; li, Z.; chen, S.; saw, P.E.; zhang, H.; bharwani, Z.; guo, Z.; shi, J.; farokhzad, O.C.Antimonene Quantum Dots: synthesis and Application as Near-Infrared Photothermal Agents for Effective Cancer Therapy.Angew.Chem.Int.Ed.2017,129,12058-12062;CN201710484246.4), CdTe (CN 201711400218.6), pbS (CN 201611050553.3) MoS </xnotran> 2 Quantum dots (CN 201650811.6) and Bi 2 S 3 Quantum dots (CN 201810939788.8), and the like. However, most quantum dots contain heavy metals, have high cytotoxicity, are strict in preparation process requirements, expensive in price and unstable in air, and are difficult to realize large-scale quantitative production, so that further application of the quantum dots in clinical treatment is limited.
Therefore, it is important to provide a photothermal agent with low cytotoxicity, simple preparation method and low cost.
Disclosure of Invention
The invention aims to provide a novel preparation method of boron quantum dots, which is prepared by taking boron powder as a raw material and using an ice salt bath ultrasonic method, and has the advantages of simple process and low equipment requirement.
The second purpose of the invention is to provide the boron quantum dot obtained by the preparation method and the application thereof, wherein the boron quantum dot has better biocompatibility and excellent photo-thermal conversion efficiency and photoacoustic imaging performance.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention firstly provides a preparation method of boron quantum dots, which comprises the following steps:
dispersing boron powder in water to obtain a boron nanosheet dispersion liquid; carrying out ultrasonic treatment on the boron nanosheet dispersion in an ice salt bath to obtain a mixed solution; and centrifuging the mixed solution, taking supernatant, and drying to obtain the boron quantum dots.
According to the invention, a simple ice salt bath ultrasonic mode is adopted to crush and crack the two-dimensional boron nanosheets into boron quantum dots with uniform sizes, and the band gaps of the boron quantum dots are obviously reduced compared with those of two-dimensional materials due to quantum confinement effect. In addition, the surface of the boron quantum dot has a large number of defects due to the existence of a plurality of unsaturated boron atoms, and the defects further modify the electronic structure of boron, so that the absorption of the boron quantum dot in a near infrared region is greatly improved, and the boron quantum dot shows excellent photo-thermal conversion performance (57%) under the irradiation of 808nm laser. The boron quantum dots have strong absorption to near-infrared light, so that the boron quantum dots have good photo-thermal treatment effect and photo-acoustic imaging performance, and the diagnosis and treatment integration of cancers is realized.
Preferably, the boron powder may be amorphous boron powder, crystalline boron powder, and/or high purity boron powder.
Preferably, the concentration of the boron powder in the boron nanoplate dispersion is 0.5-5mg/mL.
Preferably, the water is deionized water.
Preferably, the temperature of the ice salt bath is-10 to 15 ℃.
Preferably, the frequency of the ultrasonic wave is 80KHz, the power is 200-500W, and the time is 10-30h.
Preferably, the rotating speed of the centrifugation is 8000-12000 rpm/s, and the time is 10-30 min.
The boron quantum dots prepared by the preparation method are also within the protection scope of the invention.
The boron quantum dots prepared by the preparation method have the particle size of 1-10 nm and the thickness of 0.5-2 nm.
The invention further provides application of the boron quantum dot in preparation of a medicine for preventing and/or treating cancer.
The invention has the following beneficial effects:
the boron quantum dots have good photo-thermal treatment effect and can perform photo-acoustic imaging at the same time, so that the integration of cancer diagnosis and treatment is realized. The band gap of the boron quantum dots is obviously reduced, the surface has a large number of defects due to the existence of unsaturated boron, and the electronic structure of boron is further modified, so that the absorption in a near infrared region is obviously increased, and the photo-thermal conversion performance of the boron nano material is obviously improved. Meanwhile, the boron quantum dot can be effectively absorbed by tumor cells, so that the toxicity is low, and the cancer treatment effect is good. In addition, the preparation method of the boron quantum dots has the advantages of simple process, low equipment requirement, low energy consumption, environmental friendliness and capability of realizing large-scale quantitative production.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
Fig. 1 is an X-ray diffraction pattern of the boron quantum dots prepared in example 1.
FIG. 2 is a transmission electron micrograph of the boron quantum dots prepared in example 1.
Fig. 3 is a graph of photothermal curves of boron quantum dots prepared in example 1.
Fig. 4 is a photo-thermal conversion diagram of the boron quantum dots prepared in example 1.
Fig. 5 is a graph showing the cell viability of boron quantum dots prepared in example 1 at different concentrations in the presence or absence of near-infrared light irradiation.
Fig. 6 is a transmission electron micrograph of the boron quantum dots prepared in example 3.
FIG. 7 is a transmission electron micrograph of boron quantum dots prepared in example 7.
Detailed Description
In order to more clearly illustrate the invention, the invention is further described below with reference to preferred embodiments and the accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
Example 1 preparation of a boron quantum dot
The method for preparing the boron quantum dots by liquid phase stripping comprises the following steps:
1. dissolving 100mg of amorphous boron powder in 100ml of deionized water to obtain 1mg/ml of boron nanosheet dispersion liquid;
2. placing the boron nanosheet dispersion liquid obtained in the step 1 in an ultrasonic machine with the frequency of 80KHz and the power of 400W, and carrying out ice salt bath ultrasonic treatment for 10 hours at the temperature of-10 ℃ to obtain a mixed liquid;
3. and (3) centrifuging the mixed solution obtained in the step (2) for 10min in a centrifugal machine with the rotating speed of 10000rpm/s, taking supernate and placing the supernate in a drying box to remove moisture, wherein the obtained solid is the boron quantum dot.
The boron quantum dots prepared in this example were subjected to X-ray diffraction analysis, and the obtained X-ray diffraction spectrogram is shown in fig. 1, comparing fig. 1 with an XRD standard card, the diffraction peak of the boron quantum dots is consistent with the standard peak, which indicates that the structure of the boron quantum dots prepared by this method does not change.
The boron quantum dots prepared in this example were dispersed in water, and were dropped onto a copper mesh film, and observed with a transmission electron microscope, and the transmission electron microscope image is shown in fig. 2, which shows that the boron quantum dots have a uniform size, a particle size of 1 to 10nm, and a thickness of 1.5nm, indicating that the boron quantum dots prepared by liquid phase exfoliation have a significantly reduced size compared to conventional boron nanomaterials.
The boron quantum dots prepared in this example were dispersed in water at a concentration of 400ug/ml, and irradiated with a 808nm laser to detect their light-to-heat conversion properties, and the results are shown in fig. 3, which shows that the temperature of the boron quantum dots becomes higher and higher with the increase of irradiation time, and reaches 72.4 ℃ when irradiated for 10 minutes, indicating that the boron quantum dots can effectively convert light energy into heat energy under the irradiation of near-infrared light. Further, the photothermal conversion efficiency of the boron quantum dots was measured, and as a result, as shown in fig. 4, the photothermal conversion efficiency of the obtained boron quantum dots reached 57%.
After incubating HeLa cells with boron quantum dots of different concentrations (50 ug/mL, 100ug/mL, 200ug/mL, 400 ug/mL) under the conditions of near-infrared irradiation and No near-infrared irradiation respectively for 24 hours, the survival rate of the cells is calculated as shown in FIG. 5, which shows that under the condition of No near-infrared irradiation (indicated as No Laser in the figure), the survival rate of the boron quantum dots at a higher concentration of 400ug/mL is 100%, indicating that the cytotoxicity of the material is lower. However, most of HeLa cells are killed under the irradiation of near infrared light (indicated as Laser in the figure), and when the concentration of boron quantum dots is 100ug/mL, the survival rate of the cells is lower than 40%, which shows better effect of inhibiting the growth of cancer cells. The survival rate of cells is continuously reduced along with the increase of the concentration of the material, and the boron quantum dots can be used as a potential photo-thermal reagent.
Example 2 preparation of a boron quantum dot
The method for preparing the boron quantum dots by liquid phase stripping comprises the following steps:
1. dissolving 200mg of amorphous boron powder in 100ml of deionized water to obtain 2mg/ml of boron nanosheet dispersion liquid;
2. placing the boron nanosheet dispersion liquid obtained in the step 1 in an ultrasonic machine with the frequency of 80KHz and the power of 400W, and performing ice salt bath ultrasonic treatment for 10 hours at the temperature of-10 ℃ to obtain a mixed liquid;
3. and (3) centrifuging the mixed solution obtained in the step (2) for 10min in a centrifugal machine with the rotating speed of 10000rpm/s, taking supernate and placing the supernate in a drying box to remove moisture, wherein the obtained solid is the boron quantum dot.
The method described in embodiment 1 is used for detecting boron quantum dots, and the result shows that the boron quantum dots prepared by the embodiment have uniform size, 1-8 nm particle size and 0.5-2 nm thickness, and have strong absorption in the near infrared region; dispersing boron quantum dots in water, irradiating by using a 808nm laser, and measuring that the boron quantum dots can effectively convert light energy into heat energy; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is low when the boron quantum dots are measured, the photo-thermal treatment effect is good, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 3 preparation of a boron quantum dot
The method for preparing the boron quantum dots by liquid phase stripping comprises the following steps:
1. dissolving 500mg of amorphous boron powder in 100ml of deionized water to obtain 5mg/ml of boron nanosheet dispersion liquid;
2. placing the boron nanosheet dispersion liquid obtained in the step 1 in an ultrasonic machine with the frequency of 80KHz and the power of 400W, and carrying out ice salt bath ultrasonic treatment for 10 hours at the temperature of-10 ℃ to obtain a mixed liquid;
3. and (3) centrifuging the mixed solution obtained in the step (2) for 10min in a centrifuge with the rotating speed of 10000rpm/s, taking supernate and placing the supernate in a drying box to remove moisture, wherein the obtained solid is the boron quantum dot.
The method described in example 1 is used to detect boron quantum dots, and the boron quantum dots prepared in this example are observed by a transmission electron microscope, and the transmission electron microscope image is shown in fig. 6, which shows that the boron quantum dots have uniform size, 1-15 nm particle size and 1-3 nm thickness, which indicates that the boron quantum dots prepared by liquid phase stripping have a greatly reduced size compared with the conventional boron nanomaterial. The absorption in the near infrared region is strong; dispersing boron quantum dots in water, irradiating by using a 808nm laser, and measuring that the boron quantum dots can effectively convert light energy into heat energy; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is lower when the boron quantum dots are measured, the photo-thermal treatment effect is better, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 4 preparation of a boron quantum dot
Example 1 was repeated except that the conditions were changed from "the ultrasonic machine with a power of 400W" to "the ultrasonic machine with a power of 300W" in step 2, and the boron quantum dots were prepared.
The method described in the embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots prepared in the embodiment have uniform size, 1-12 nm particle size and 1-2.5 nm thickness, and have strong absorption in a near infrared region; dispersing boron quantum dots in water, irradiating by using a 808nm laser, and measuring that the boron quantum dots can effectively convert light energy into heat energy; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is low when the boron quantum dots are measured, the photo-thermal treatment effect is good, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 5 preparation of a boron quantum dot
Example 1 was repeated except that "in the ultrasonic machine with a power of 400W" in step 2 "was changed to" in the ultrasonic machine with a power of 500W ", and the other conditions were not changed to prepare boron quantum dots.
The method of embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-8 nm particle size and 0.5-1.5 nm thickness, and have strong absorption in a near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is low when the boron quantum dots are measured, the photo-thermal treatment effect is good, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 6 preparation of a boron quantum dot
Example 1 was repeated, except that "rotation speed of 10000rpm/s" in step 3 was changed to "rotation speed of 11000rpm/s". And (4) changing the centrifugation for 10min in the step (3) into the centrifugation for 20min, and keeping the other conditions unchanged to prepare the boron quantum dots.
The method of embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-10 nm particle size and 0.5-2 nm thickness, and have strong absorption in a near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is lower when the boron quantum dots are measured, the photo-thermal treatment effect is better, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 7 preparation of a boron quantum dot
Example 1 was repeated except that "rotation speed of 10000rpm/s" in step 3 was changed to 12000rpm/s ", that" centrifugation 10min "in step 3 was changed to" centrifugation 30min ", and that the remaining conditions were not changed, to prepare boron quantum dots.
The method described in embodiment 1 is used for detecting boron quantum dots, the boron quantum dots prepared in this embodiment are observed by a transmission electron microscope, and a transmission electron microscope image is shown as fig. 7, which shows that the boron quantum dots have uniform size, 1-8 nm particle size, 0.5-1.5 nm thickness and strong absorption in the near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is low when the boron quantum dots are measured, the photo-thermal treatment effect is good, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 8 preparation of a boron quantum dot
Example 7 was repeated except that "amorphous boron powder" in step 1 was changed to "crystalline boron powder", and the remaining conditions were not changed to prepare boron quantum dots.
The method of embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-10 nm particle size and 0.5-3 nm thickness, and have strong absorption in a near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is lower when the boron quantum dots are measured, the photo-thermal treatment effect is better, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown. .
Example 9 preparation of a boron quantum dot
Example 7 was repeated except that "amorphous boron powder" in step 1) was changed to "high-purity boron powder". And (5) keeping the other conditions unchanged, and preparing the boron quantum dots.
The method of embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-12 nm particle size, 0.5-2 nm thickness and strong absorption in a near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is lower when the boron quantum dots are measured, the photo-thermal treatment effect is better, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 10 preparation of a boron quantum dot
Example 9 was repeated with the difference that "after 10h of ice salt bath sonication at-10 ℃" in step 1) was changed to "after 20h of ice salt bath sonication at 0 ℃. And the other conditions are unchanged, and the boron quantum dots are prepared.
The method of embodiment 1 is used for detecting boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-9 nm particle size and 1-3.5 nm thickness, and have strong absorption in the near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is low when the boron quantum dots are measured, the photo-thermal treatment effect is good, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Example 11 preparation of a boron quantum dot
Example 9 was repeated with the difference that "after 10h of ice salt bath sonication at-10 ℃" in step 1) was changed to "after 20h of ice salt bath sonication at 5 ℃. And the other conditions are unchanged, and the boron quantum dots are prepared.
The method of embodiment 1 is used for detecting the boron quantum dots, and the boron quantum dots obtained by the embodiment have uniform size, 1-12 nm particle size, 0.5-3 nm thickness and strong absorption in a near infrared region; dispersing boron quantum dots in water, and irradiating by using a 808nm laser, wherein the boron quantum dots have higher photo-thermal conversion efficiency; the boron quantum dots are used for incubating HeLa cells, the cytotoxicity of the boron quantum dots is lower when the boron quantum dots are measured, the photo-thermal treatment effect is better, the cyclicity is good, and the excellent photo-acoustic imaging performance is shown.
Comparative example 1
The preparation method is basically the same as that of the example 1, except that the 'ice salt bath ultrasound at-15 ℃ for 10 h' in the step 2) is replaced by 'ultrasound at room temperature for 10 h', the rest conditions are unchanged, the particle size of the obtained boron quantum dot is larger, about 20-50 nm, the thickness is 1-5 nm, the absorption in a near infrared region is weaker, the photothermal conversion efficiency is lower, the photothermal treatment effect is poor, and the photoacoustic imaging cannot be carried out.
Comparative example 2
The preparation method is basically the same as that of the example 1, except that the boron nanosheet dispersion obtained in the step 1) is directly used for subsequent exploration. The photothermal conversion efficiency of the boron nanosheets is low, the phototherapy effect is general, and photoacoustic imaging cannot be carried out.
Comparative example 3
The preparation method is basically the same as that of the embodiment 1, except that the 'ice salt bath ultrasound at-15 ℃ for 10 h' in the step 2) is replaced by 'ice salt bath ultrasound at-15 ℃ for 5 h', the rest conditions are not changed, the particle size of the obtained boron quantum dots is larger, about 20-30 nm, the thickness is 1-5 nm, the absorption in a near infrared region is weaker, the photothermal conversion efficiency is lower, the photothermal treatment effect is poor, and the photoacoustic imaging cannot be carried out.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all embodiments may not be exhaustive, and all obvious variations or modifications may be included within the scope of the present invention.
Claims (4)
1. A preparation method of a boron quantum dot is characterized by comprising the following steps:
dispersing boron powder in water to obtain a boron nanosheet dispersion liquid; carrying out ultrasonic treatment on the boron nanosheet dispersion liquid in an ice salt bath to obtain a mixed liquid; centrifuging the mixed solution, taking supernatant, and drying to obtain boron quantum dots;
the temperature of the ice salt bath is-10 ℃; the frequency of the ultrasonic is 80KHz, the power is 200 to 500W, and the time is 10 to 30 hours.
2. The production method according to claim 1, wherein the boron powder is an amorphous boron powder, a crystalline boron powder, and/or a high purity boron powder.
3. The production method according to claim 1 or 2, characterized in that the concentration of the boron powder in the boron nanosheet dispersion is 0.5-5mg/mL.
4. The method for preparing the nano-particles according to claim 1, wherein the rotation speed of the centrifugation is 8000 to 12000rpm/s, and the time is 10 to 30min.
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