CN113693957A - Complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and preparation method thereof - Google Patents
Complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/24—Phosphorous; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
- A61K8/492—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid having condensed rings, e.g. indol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention provides a complete culture medium base solution preparation for cosmetics for proliferation, growth and restoration of skin cells and a preparation method thereof, relates to the technical field of cell culture media and preparation methods thereof, and aims to solve the problem that the culture medium base solution of cells in culture cannot maintain the efficient activity of the cells for a long time.
Description
Technical Field
The invention relates to the technical field of cell culture media and preparation methods thereof, in particular to a complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and a preparation method thereof.
Background
The complete culture medium is called complete culture medium after adding substances such as serum and the like into the basic culture medium, and is also called (serum) cell culture medium. The basic culture medium can only maintain the survival of cells, and a natural culture medium needs to be added for the growth and the propagation of the cells, and a certain amount of kinetin needs to be added in a culture solution. The complete medium can be divided into a cell growth medium and a cell maintenance medium according to the amount of serum added, and is used for different cells and different researches.
The complete culture medium, i.e. cell growth medium (liquid), is used to maintain the growth and proliferation of cells, and is a kind of microbiological culture medium commonly used in the culture medium, which is prepared by adding natural substances rich in amino acids, vitamins and bases, i.e. growth factors, into a minimal medium.
The complete medium can be divided into a cell growth medium and a cell maintenance medium according to the amount of serum added, and is used for different cells and different researches. The cell growth medium (liquid) is used to maintain the growth and proliferation of cells and contains a large proportion of serum. The growth medium is the most important culture medium in the work of culturing cells. The composition is as follows: 80-90% of basal culture medium, 10-20% of serum (multipurpose serum protein) and kinetin. Culture medium (liquid) this is a composition of a culture liquid suitable for general cells. For the cultivation of a particular cell, an adaptation medium is also selected, possibly supplemented with components lacking in the minimal medium or required by the cell. The maintenance medium is a culture solution for maintaining slow growth or immobility of cells, and has little serum content.
Disclosure of Invention
In view of the problems in the prior art, the invention discloses a complete culture medium liquid preparation of cosmetics for skin cell proliferation and repair, which adopts the technical scheme that the final concentrations of all components in a finished product are respectively as follows: 38-46mg/L serine, 24-36mg/L glycine, 60-72mg/L alanine, 70-78mg/L valine, 100-110mg/L isoleucine, 100-110mg/L leucine, 60-80mg/L proline, 240-250mg/L arginine, 125-135mg/L aspartic acid, 90-100mg/L threonine, 142-152mg/L lysine, 580-590mg/L glutamic acid, 38-46mg/L histidine, 25-35mg/L methionine, 12-20mg/L tryptophan, 60-66mg/L cystine, 100-108mg/L tyrosine, 160mg/L serum protein 140, 240mg/L fibronectin 220, 240mg/L tryptophan, Oligopeptide, kinetin 50-70mg/L, vitamin and dihydrated sodium dihydrogen phosphate 120-130mg/L, complete culture medium is adopted, cells can proliferate, and after the cells obtain activity, the effects of repairing skin, resisting aging and increasing skin elasticity are achieved, flabby and drooping skin is enabled to be compact and plump, wrinkles are filled with protein components, and the wrinkles and the skin are prevented from being wrinkled and moistened.
As a preferred embodiment of the present invention, the oligopeptides include oligopeptide-2 and oligopeptide-4, the oligopeptide-2 and oligopeptide-4 are 30-40mg/L and 40-50mg/L respectively, and the oligopeptide-2 and oligopeptide-4 are added into a complete culture medium, have extremely high skin permeability, are easily absorbed by cells, enhance the activity of the cells, and reduce the average age of skin tissues; promoting synthesis of collagen and collagenase, secreting collagen substances, hyaluronic acid and glycoprotein, moistening skin, improving skin elasticity, reducing skin wrinkles and preventing skin aging.
In a preferable embodiment of the invention, the vitamin is vitamin B6, the vitamin B6 is 2-8mg/L, and the vitamin B6 is added into the complete culture medium, so that the vitamin B plays a role in regulating the metabolic process of cell protein, contributes to energy production and enables cells to keep activity.
A method for preparing a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells comprises the following steps:
step 1, weighing raw materials according to a volume ratio, and uniformly mixing the raw materials in a disinfected beaker to obtain a mixed raw material;
step 2, adding clear water into the beaker to 800 milliliters, and stirring and dissolving by using a sterilized stirring rod;
step 3, adding phosphate buffer solution into the beaker to enable the concentration of the phosphate buffer solution to reach 2.5g/L, stirring and dissolving the phosphate buffer solution by using a stirring rod,
step 4, adjusting the pH value in the beaker, and adding water into the beaker to 1 liter to obtain a culture medium; step 5, filtering the culture medium; obtaining a complete culture medium;
and 6, placing the complete culture medium in a light-shielding environment at 3-7 ℃ for storage, wherein the complete culture medium is a nutrient medium prepared by adding natural substances such as rich amino acids, proteins, oligopeptides, vitamins and bases into a basic culture medium, namely adding growth factors, can be used for meeting the growth and reproduction requirements of various cell nutrients of the repaired microorganism, particularly adding kinetin for achieving activity, thereby improving the activity of cells, and the complete culture medium, namely a cell growth culture medium (liquid) is used for maintaining the growth and the reproduction of the cells and is beneficial to improving the activity of the cells.
In a preferred embodiment of the present invention, the beaker and the stirring rod in the step 1 and the step 2 are treated by high temperature cooking and ultraviolet lamp sterilization.
As a preferable technical scheme of the invention, the PH in the step 4 is only adjusted by adopting 1moL/L sodium hydroxide solution or 1moL/L hydrochloric acid solution.
As a preferable technical scheme of the invention, the PH value in the beaker is 7.2-7.4.
In a preferred embodiment of the present invention, the filtration in step 5 is performed by performing filtration sterilization at a positive pressure using a 0.2 μm filter membrane, and performing filtration sterilization at a positive pressure using a filter membrane, and the filter membranes used are mostly PES, nylon, polycarbonate, cellulose acetate, cellulose nitrate, PTFE, ceramics, and the like. In general, positive pressure filtration is adopted, and compared with negative pressure filtration, positive pressure filtration has the advantages of high flow rate, fast filtration, difficult pollution and the like, and can avoid a large amount of bubbles generated by protein.
The invention has the beneficial effects that: the complete culture medium is a nutrient medium prepared by adding natural substances such as rich amino acids, proteins, oligopeptides, vitamins and basic groups into a basic culture medium, namely adding growth factors, can be used for meeting the growth and reproduction requirements of various cell nutrients of repairing microorganisms, and specially adding kinetin for achieving activity so as to improve the activity of cells, and the complete culture medium, namely a cell growth culture medium (liquid) is used for maintaining the growth and the multiplication of the cells and is beneficial to improving the activity of the cells.
Furthermore, a complete culture medium is adopted, so that cells can be proliferated, and after the cells are activated, the effects of repairing skin, resisting aging and increasing skin elasticity are achieved, flabby and drooping skin is compact and plump, wrinkles are filled with protein components, and the skin is wrinkle-resistant and moist.
Furthermore, the vitamin B6 is added into the complete culture medium, so that the complete culture medium has a regulation effect in the metabolic process of cell proteins, contributes to energy generation and enables cells to keep activity; the oligopeptide-2 and the oligopeptide-4 are added into the complete culture medium, so that the skin permeability is extremely high, the cells can easily utilize and absorb the oligopeptide-2 and the oligopeptide-4, and the activity of the cells is enhanced.
Detailed Description
Example 1
The invention discloses a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells, which adopts the technical scheme that the final concentration of each component in a finished product is as follows: serine 38mg/L, glycine 24mg/L, alanine 60mg/L, valine 70mg/L, isoleucine 100mg/L, leucine 100mg/L, proline 60mg/L, arginine 240mg/L, aspartic acid 125mg/L, threonine 90mg/L, lysine 142mg/L, glutamic acid 580mg/L, histidine 38mg/L, methionine 25mg/L, tryptophan 12mg/L, cystine 60mg/L, tyrosine 100mg/L, serum protein 140mg/L, fibronectin 220mg/L, oligopeptide, kinetin 50mg/L, vitamin and sodium dihydrogen phosphate dihydrate 120 mg/L.
As a preferred technical scheme of the invention, the oligopeptide comprises oligopeptide-2 and oligopeptide-4, the oligopeptide-2 and the oligopeptide-4 are respectively 30mg/L and 40mg/L, and the oligopeptides have good repairing and nursing effects on damaged skin, sensitive skin and wounded skin.
According to a preferable technical scheme of the invention, the vitamin is vitamin B6, the vitamin B6 is 2mg/L, a complete culture medium is adopted, cells can proliferate, and after the cells are activated, the effects of repairing skin, resisting aging and increasing skin elasticity are achieved, flabby and drooping skin is made to be compact and plump, wrinkles are filled with protein components, and the wrinkles are prevented from being excessively moistened.
A method for preparing a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells comprises the following steps:
step 1, weighing raw materials according to a volume ratio, and uniformly mixing the raw materials in a disinfected beaker to obtain a mixed raw material; when the raw materials are weighed, a rubber dropper is adopted to absorb various raw materials, and the raw materials are added into a beaker for mixing;
step 2, adding clear water into the beaker to 800 milliliters, and stirring and dissolving by using a sterilized stirring rod; when adding clear water into the beaker, adding clear water while slightly stirring the stirring rod attached to the inner wall of the beaker,
step 3, adding phosphate buffer solution into the beaker to enable the concentration of the phosphate buffer solution to reach 2.5g/L, stirring and dissolving the phosphate buffer solution by using a stirring rod,
step 4, adjusting the pH value in the beaker, and adding water into the beaker to 1 liter to obtain a culture medium; the pH in the beaker can only not exceed 7.4 at the maximum;
step 5, filtering the culture medium; obtaining a complete culture medium, and cleaning mixed bacteria in the culture medium through bacteria filtration treatment;
and 6, storing the complete culture medium in a dark environment at 3 ℃.
As a preferred technical scheme of the invention, the beaker and the stirring rod in the step 1 and the step 2 are subjected to high-temperature cooking or ultraviolet lamp sterilization treatment, and the ultraviolet lamp sterilization is adopted, so that the sterilization efficiency is higher, the broad spectrum is the highest, almost all bacteria and viruses can be killed efficiently, and no secondary pollution is caused; adopts high-pressure sterilization, has simple operation and short sterilization time, and can kill virus.
As a preferable technical scheme of the invention, the PH in the step 4 is only adjusted by adopting 1moL/L sodium hydroxide solution or 1moL/L hydrochloric acid solution, and the PH of the complete culture medium is adjusted by adopting the sodium hydroxide solution or the hydrochloric acid solution, so that the PH value of the complete culture medium can be efficiently adjusted, and other ions are not introduced.
In a preferred embodiment of the present invention, the PH in the beaker is 7.2.
In a preferred embodiment of the present invention, the filtration in step 5 is performed by performing filtration sterilization at a positive pressure using a 0.2 μm filter membrane, and performing filtration sterilization at a positive pressure using a filter membrane, and the filter membranes used are mostly PES, nylon, polycarbonate, cellulose acetate, cellulose nitrate, PTFE, ceramics, and the like. In general, positive pressure filtration is adopted, and compared with negative pressure filtration, positive pressure filtration has the advantages of high flow rate, fast filtration, difficult pollution and the like, and can avoid a large amount of bubbles generated by protein.
Example 2
The invention discloses a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells, which adopts the technical scheme that the final concentration of each component in a finished product is as follows: serine 46mg/L, glycine 36mg/L, alanine 72mg/L, valine 78mg/L, isoleucine 110mg/L, leucine 110mg/L, proline 80mg/L, arginine 250mg/L, aspartic acid 135mg/L, threonine 100mg/L, lysine 152mg/L, glutamic acid 590mg/L, histidine 46mg/L, methionine 35mg/L, tryptophan 20mg/L, cystine 66mg/L, tyrosine 108mg/L, serum protein 160mg/L, fibronectin 240mg/L, oligopeptides, kinetin 70mg/L, vitamins and sodium dihydrogen phosphate dihydrate 130 mg/L.
As a preferable technical scheme, the oligopeptide comprises oligopeptide-2 and oligopeptide-4, the oligopeptide-2 and the oligopeptide-4 are 40mg/L and 50mg/L respectively, and the oligopeptide is a high-activity small molecular peptide which can clean the skin and promote the self-repairing force of the skin, so that the skin is restored to be fine and smooth.
According to a preferable technical scheme of the invention, the vitamin is vitamin B6, the vitamin B6 is 8mg/L, and the vitamin B can regulate lipid metabolism of the skin, relieve xerosis cutis and maintain the integrity of the skin.
A method for preparing a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells comprises the following steps:
step 1, weighing raw materials according to a volume ratio, and uniformly mixing the raw materials in a disinfected beaker to obtain a mixed raw material;
step 2, adding clear water into the beaker to 800 milliliters, and stirring and dissolving by using a sterilized stirring rod;
step 3, adding phosphate buffer solution into the beaker to enable the concentration of the phosphate buffer solution to reach 2.5g/L, stirring and dissolving the phosphate buffer solution by using a stirring rod,
step 4, adjusting the pH value in the beaker, and adding water into the beaker to 1 liter to obtain a culture medium; step 5, filtering the culture medium; obtaining a complete culture medium;
and 6, storing the complete culture medium in a dark environment at the temperature of 3-7 ℃.
As a preferable technical scheme of the invention, the beaker and the stirring rod in the step 1 and the step 2 are subjected to high-temperature cooking and ultraviolet lamp sterilization treatment, and high-pressure sterilization is adopted, so that the operation is simple, the sterilization time is short, and viruses can be killed.
As a preferable technical scheme of the invention, the PH in the step 4 is only adjusted by adopting 1moL/L sodium hydroxide solution or 1moL/L hydrochloric acid solution, and the PH of the complete culture medium is adjusted by adopting the sodium hydroxide solution or the hydrochloric acid solution, so that the method is efficient and rapid.
In a preferred embodiment of the present invention, the PH in the beaker is 7.4.
In a preferred embodiment of the present invention, the step 5 of filtering is performed by using a 0.2 μm filter membrane for positive pressure filtration sterilization.
Example 3
The invention discloses a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells, which adopts the technical scheme that the final concentration of each component in a finished product is as follows: serine 38-46mg/L, glycine 24-36mg/L, alanine 66mg/L, valine 74mg/L, isoleucine 105mg/L, leucine 105mg/L, proline 70mg/L, arginine 245mg/L, aspartic acid 130mg/L, threonine 95mg/L, lysine 146mg/L, glutamic acid 585mg/L, histidine 42mg/L, methionine 30mg/L, tryptophan 16mg/L, cystine 63mg/L, tyrosine 104mg/L, serum protein 150mg/L, fibronectin 230mg/L, oligopeptide, kinetin 60mg/L, vitamin and sodium dihydrogen phosphate dihydrate 125 mg/L.
As a preferred technical scheme of the invention, the oligopeptide comprises oligopeptide-2 and oligopeptide-4, the oligopeptide-2 and the oligopeptide-4 are 35mg/L and 45mg/L respectively, and the oligopeptide-2 and the oligopeptide-4 are added into a complete culture medium, so that the composition has extremely high skin permeability, is easily utilized and absorbed by cells, and enhances the activity of the cells.
In a preferred embodiment of the present invention, the vitamin is vitamin B6, the vitamin B6 is 5mg/L, and the vitamin B6 is added to the complete medium, which plays a role in regulating the metabolic process of cell proteins, contributes to energy production, and allows the cells to maintain activity.
A method for preparing a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells comprises the following steps:
step 1, weighing raw materials according to a volume ratio, and uniformly mixing the raw materials in a disinfected beaker to obtain a mixed raw material;
step 2, adding clear water into the beaker to 800 milliliters, and stirring and dissolving by using a sterilized stirring rod;
step 3, adding phosphate buffer solution into the beaker to enable the concentration of the phosphate buffer solution to reach 2.5g/L, stirring and dissolving the phosphate buffer solution by using a stirring rod,
step 4, adjusting the pH value in the beaker, and adding water into the beaker to 1 liter to obtain a culture medium; step 5, filtering the culture medium; obtaining a complete culture medium;
and 6, storing the complete culture medium in a dark environment at the temperature of 5 ℃.
As a preferred technical scheme of the invention, the beaker and the stirring rod in the step 1 and the step 2 are subjected to high-temperature cooking and ultraviolet lamp sterilization treatment, and the beaker and the stirring rod are sterilized by adopting an ultraviolet lamp, so that the sterilization efficiency is higher, the broad spectrum is the highest, almost all bacteria and viruses can be killed efficiently, and no secondary pollution is caused.
As a preferable technical scheme of the invention, the PH in the step 4 is only adjusted by adopting 1moL/L sodium hydroxide solution or 1moL/L hydrochloric acid solution.
In a preferred embodiment of the present invention, the PH in the beaker is 7.3.
In a preferred embodiment of the present invention, the step 5 of filtering is performed by using a 0.2 μm filter membrane for positive pressure filtration sterilization.
Components not described in detail herein are prior art.
Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (8)
1. A complete culture medium liquid preparation of cosmetics for proliferating, growing and repairing skin cells, which is characterized in that: the final concentration of each component in the finished product is as follows: 38-46mg/L serine, 24-36mg/L glycine, 60-72mg/L alanine, 70-78mg/L valine, 100-110mg/L isoleucine, 100-110mg/L leucine, 60-80mg/L proline, 240-250mg/L arginine, 125-135mg/L aspartic acid, 90-100mg/L threonine, 142-152mg/L lysine, 580-590mg/L glutamic acid, 38-46mg/L histidine, 25-35mg/L methionine, 12-20mg/L tryptophan, 60-66mg/L cystine, 100-108mg/L tyrosine, 160mg/L serum protein 140, 240mg/L fibronectin 220, 240mg/L tryptophan, Oligopeptide, kinetin 50-70mg/L, vitamin and sodium dihydrogen phosphate dihydrate 120-130 mg/L.
2. The cosmetic complete medium formulation for skin cell proliferation growth and repair according to claim 1, wherein: the oligopeptide comprises oligopeptide-2 and oligopeptide-4, and the oligopeptide-2 and the oligopeptide-4 are respectively 30-40mg/L and 40-50 mg/L.
3. The cosmetic complete medium formulation for skin cell proliferation growth and repair according to claim 1, wherein: the vitamin is vitamin B6, and the vitamin B6 is 2-8 mg/L.
4. A method for preparing a complete culture medium liquid preparation of cosmetics for proliferation, growth and repair of skin cells comprises the following steps:
step 1, weighing raw materials according to a volume ratio, and uniformly mixing the raw materials in a disinfected beaker to obtain a mixed raw material;
step 2, adding clear water into the beaker to 800 milliliters, and stirring and dissolving by using a sterilized stirring rod;
step 3, adding phosphate buffer solution into the beaker to enable the concentration of the phosphate buffer solution to reach 2.5g/L, stirring and dissolving the phosphate buffer solution by using a stirring rod,
step 4, adjusting the pH value in the beaker, and adding water into the beaker to 1 liter to obtain a culture medium;
step 5, filtering the culture medium; obtaining a complete culture medium;
and 6, storing the complete culture medium in a dark environment at the temperature of 3-7 ℃.
5. The method for preparing a complete medium formulation for cosmetic preparation for skin cell proliferation and repair according to claim 4, wherein: and (3) adopting high-temperature cooking or ultraviolet lamp sterilization treatment for the beaker and the stirring rod in the step (1) and the step (2).
6. The method for preparing a complete medium formulation for cosmetic preparation for skin cell proliferation and repair according to claim 4, wherein: and (4) only adjusting the pH in the step (4), and adjusting by adopting 1moL/L sodium hydroxide solution or 1moL/L hydrochloric acid solution.
7. The method for preparing a complete medium formulation for cosmetic preparation for skin cell proliferation and repair as claimed in claim 6, wherein: the pH value in the beaker is 7.2-7.4.
8. The method for preparing a complete medium formulation for cosmetic preparation for skin cell proliferation and repair according to claim 4, wherein: and (5) performing bacteria filtration treatment in the step 5, and performing positive pressure filtration sterilization by adopting a 0.2-micron filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111021063.1A CN113693957A (en) | 2021-09-01 | 2021-09-01 | Complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111021063.1A CN113693957A (en) | 2021-09-01 | 2021-09-01 | Complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and preparation method thereof |
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| Publication Number | Publication Date |
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| CN113693957A true CN113693957A (en) | 2021-11-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111021063.1A Withdrawn CN113693957A (en) | 2021-09-01 | 2021-09-01 | Complete cosmetic culture medium liquid preparation for skin cell proliferation growth and repair and preparation method thereof |
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| Country | Link |
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| CN (1) | CN113693957A (en) |
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- 2021-09-01 CN CN202111021063.1A patent/CN113693957A/en not_active Withdrawn
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