CN113693068A - Composition for improving cold resistance of cucumber seedlings and application thereof - Google Patents

Composition for improving cold resistance of cucumber seedlings and application thereof Download PDF

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CN113693068A
CN113693068A CN202111108926.9A CN202111108926A CN113693068A CN 113693068 A CN113693068 A CN 113693068A CN 202111108926 A CN202111108926 A CN 202111108926A CN 113693068 A CN113693068 A CN 113693068A
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aba
cacl
composition
cucumber
solution
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闫妍
冯倩
于贤昌
孙敏涛
李衍素
贺超兴
王君
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Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C7/00—Sowing
    • A01C7/06—Seeders combined with fertilising apparatus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides

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Abstract

The invention relates to agricultural technology, in particular to a composition for improving cold resistance of cucumber seedlings and application thereof. The active ingredients of the composition comprise ABA and CaCl2Can effectively relieve the accumulation of membrane lipid peroxidation products, increase the activity of cucumber seedling antioxidant enzyme under low temperature stress, effectively relieve the damage of low temperature to cucumber seedling, improve the low temperature resistance of cucumber seedling, avoid the inhibiting effect of ABA (abscisic acid) on the growth of seedling leaves and CaCl2Leaf wilting and yellowing easily occur.

Description

Composition for improving cold resistance of cucumber seedlings and application thereof
Technical Field
The invention relates to agricultural technology, in particular to a composition for improving cold resistance of cucumber seedlings and application thereof.
Background
In recent years, the facility agriculture has been rapidly developed, and the total area of the facility vegetables has been expanded. Cucumber (Cucumis sativus L.) is a cold sensitive plant originated from tropical and subtropical zones, and low-temperature weak light becomes one of the major bottlenecks limiting the production development of facility cucumbers in winter and spring.
The suitable growth temperature range of the cucumber is 25-30 ℃ in the daytime and 13-15 ℃ at night. However, the problems of long-term low temperature (<20 ℃/8-12 ℃ C., day/night) and short-term critical low temperature (15 ℃/4-8 ℃ C., day/night) commonly exist in cucumber cultivation in winter and spring in northern areas. Low temperature stress has become an important adversity factor that restricts high yield and high quality of cucumbers. The existing research shows that the germination rate of cucumber seeds can be reduced under low temperature stress, the edges of leaves in the seedling stage are yellowed, withered and involuted, the fertilization rate in the flowering stage and the fruit setting rate in the fruiting stage are reduced, the melons and deformed melons are serious, and the fruits are easy to rot and have reduced quality in the storage and transportation stages. The low-temperature stress in facility cultivation is mainly cold damage, and how to improve the low-temperature resistance of the cucumbers and relieve the low-temperature damage in the facility cultivation in winter and spring is still an urgent problem to be solved.
The existing research shows that the cold acclimation process of plants can be simulated by applying ABA (abscisic acid) externally, and the freezing resistance of plants such as wheat, arabidopsis thaliana and the like is improved. However, it has also been reported that ABA does not contribute to the anti-freezing capacity of some plants. Therefore, the role in the response of plants to low temperatures is not clear.
In practical application, the ABA or the CaCl is independently used under the influence of multiple factors such as species difference, temperature change, illumination change and the like2The cold resistance effect on cucumbers is not ideal.
Disclosure of Invention
The inventor passes a large number ofThe research shows that ABA and CaCl2The combined use can obviously improve the cold resistance of cucumber seedlings. The invention is particularly proposed.
The invention firstly provides a composition for improving the cold resistance of cucumber seedlings, which comprises ABA and CaCl as active ingredients2。
The composition for improving the cold resistance of cucumber seedlings comprises ABA and CaCl as active ingredients2The components are as follows.
In some embodiments, ABA and CaCl are present in the composition2The weight ratio of (A) to (B) is 6.5-7.5: 100.
In some embodiments, ABA and CaCl are present in the composition2In a weight ratio of 7: 100.
In some embodiments, the composition that improves cold resistance of cucumber seedlings is a solution.
In some embodiments, the composition is ABA and CaCl2In which each liter (L) of ABA and CaCl2The mixed solution of (A) contains 32.5-37.5mg of ABA and 500mg of CaCl2。
In some embodiments, the composition is ABA and CaCl2The mixed solution of (1), wherein each liter of ABA and CaCl2The mixed solution of (A) contained 35mg of ABA and 500mg of CaCl2。
According to the embodiment of the invention, the composition for improving the cold resistance of cucumber seedlings is ABA solution and CaCl2A solution; wherein the concentration of ABA solution is 15-35mg/L, such as 15mg/L, 20mg/L, 25mg/L, 30mg/L, 35 mg/L; CaCl2The solution concentration is 500-1500mg/L, such as 500mg/L, 600mg/L, 800mg/L, 900mg/L, 1000mg/L, 1200mg/L, 1300mg/L, 1500 mg/L.
According to the embodiment of the invention, the composition for improving the cold resistance of cucumber seedlings is ABA solution and CaCl2A solution; wherein the concentration of the ABA solution is 25-35 mg/L; CaCl2The solution concentration is 1000-1500 mg/L.
According to the embodiment of the invention, the composition for improving the cold resistance of cucumber seedlings is ABA solution and CaCl2A solution; wherein the concentration of the ABA solution is 35 mg/L; CaCl2The concentration of the solution was 500 mg/L.
When the composition for improving the cold resistance of cucumber seedlings, which is used as a solution, is used in the embodiment of the invention, ABA and CaCl are preferably added before use2Dissolving in water to obtain solution.
If not specifically stated, when the composition for improving the cold resistance of cucumber seedlings according to the examples of the present invention is a solution, the solvent is water.
The embodiment of the invention also provides application of the composition in improving the cold resistance of cucumber seedlings.
The embodiment of the invention also provides application of the composition in any one or more aspects of improving the antioxidase activity of leaves of cucumber seedlings, reducing the cold injury index of the cucumber seedlings, reducing the MDA content of the leaves and reducing the relative conductivity of the leaves.
The embodiment of the invention also provides a method for improving the cold resistance of cucumber seedlings, which comprises the following steps: providing the above composition in the form of a solution; the composition is sprayed on the leaf surfaces of cucumber seedlings.
According to an embodiment of the invention, the cucumber variety is 'Zhongnong 26'.
According to an embodiment of the invention, the cucumber seedling is a two-leaf and one-heart seedling.
According to an embodiment of the invention, the composition is sprayed in the morning (e.g. 8-12 o 'clock, in particular e.g. 9 o' clock) during cucumber seedling stage. Generally, the spraying can be continuously carried out for 3 to 5 days.
According to an embodiment of the invention, the composition is sprayed in an amount of 2-6mL per strain, for example 3mL per strain.
The composition provided by the invention is simple and convenient to use, small in dosage and small in side effect, and can effectively eliminate the accumulation of toxic substance malondialdehyde, reduce the exosmosis of electrolyte, effectively relieve the accumulation of membrane lipid peroxidation products, increase the activity of antioxidant enzymes of cucumber seedlings under low-temperature stress, effectively relieve the damage of low temperature to the cucumber seedlings and improve the low-temperature resistance of the cucumber seedlings.
The invention has the beneficial effects that:
(1) the composition for improving the low temperature resistance of the cucumber seedlings disclosed by the invention is prepared by spraying ABA and CaCl with proper concentrations from an external source2The solution can beEffectively eliminates the accumulation of toxic substance malondialdehyde content, reduces the exosmosis of electrolyte, effectively relieves the accumulation of membrane lipid peroxidation products, increases the activity of antioxidant enzyme of cucumber seedlings under low-temperature stress, and effectively relieves the damage of low temperature to the cucumber seedlings.
(2) The technical scheme of the invention has the advantages of simple preparation, convenient use, less use amount than single use amount, better effect, obvious synergistic effect and no conventional speculation of the technical personnel in the field.
(3) The technical scheme of the invention avoids the inhibiting effect of ABA on the growth of the seedling leaves and CaCl when the ABA is used alone2Leaf wilting and yellowing easily occur.
(4) The composition of the invention belongs to a non-toxic substance, is used in the processes of plant production and food processing, and has no potential safety hazard.
(5) The invention is also suitable for solanaceous seedlings, has wide application range and can not generate drug resistance of plants.
Drawings
FIG. 1 shows Experimental examples ABA and CaCl of the invention2Influence of single spraying on phenotype of cucumber seedlings under low-temperature stress.
FIG. 2 shows Experimental examples ABA and CaCl of the invention2Influence of single spraying on the cold injury index and MDA content of cucumber seedlings under low-temperature stress. The difference between treatments is significant for different lower case letters (n-3, P)<0.05)。
FIG. 3 shows Experimental examples ABA and CaCl of the present invention2Influence of single spraying on the relative conductivity of leaves of cucumber seedlings under low-temperature stress. The difference between treatments is significant for different lower case letters (n-3, P)<0.05)。
FIG. 4 shows Experimental examples ABA and CaCl of the present invention2Influence of single spraying on the antioxidant enzyme activity of cucumber seedling leaves under low-temperature stress. The difference between treatments is significant for different lower case letters (n-3, P)<0.05)。
FIG. 5 shows the effect of the combination treatment of the invention on the phenotype of cucumber seedlings under low temperature stress.
FIG. 6 shows the effect of the compound combination treatment of the experimental examples of the present invention on the chilling injury index and MDA content of cucumber seedlings under low temperature stress. Different lower case letters indicate significant differences between treatments (n-3, P < 0.05).
FIG. 7 shows the effect of the compound combination treatment of the experimental examples of the present invention on the relative conductivity of cucumber seedling leaves under low temperature stress. Different lower case letters indicate significant differences between treatments (n-3, P < 0.05).
FIG. 8 shows the effect of the compound combination treatment of the experimental examples of the present invention on the antioxidant activity of cucumber seedling leaves under low temperature stress. Different lower case letters indicate significant differences between treatments (n-3, P < 0.05).
Detailed Description
The present invention will be described in further detail with reference to specific examples, which should not be construed as limiting the scope of the present invention.
Not specifically stated, the following "15 mg/L ABA +1000mg/L CaCl2"indicates ABA and CaCl in solution2The concentration of (b) is 15mg/L and 1000mg/L respectively, and the solvent is water. Other similar forms are synonymous.
Examples of the experiments
1 materials and methods
1.1 test materials
The experiment is carried out in the sunlight greenhouse of vegetable and flower research institute of Chinese academy of agricultural sciences in 5 months-2021 in 2020 and 5 months. The test material is cucumber 'Zhongnong 26', the seeds are purchased from technical and scientific Co., Ltd, for testing reagents ABA (abscisic acid) and CaCl2Purchased from Biotech, Inc., of the North Kyowa transocean, Inc. The substrate is turf and vermiculite (volume ratio is 2:1), and the nutrition bowl (size is 7cm multiplied by 8 cm).
1.2 design of the experiment
Soaking cucumber seeds in warm soup of 'Zhongnong No. 26' as a test material at 55 ℃ for 30min, soaking the soaked cucumber seeds at room temperature for 4h, placing the soaked cucumber seeds in a thermostat at 28 ℃ for accelerating germination for 24h, selecting the seeds with consistent germination, sowing the seeds in a 32-hole seedling culture hole tray, placing the seedling culture substrates of turf and vermiculite (the volume ratio is 2:1) in a phytotron of vegetable and flower institute of Chinese academy of agricultural sciences for culture under the conditions of day time (24 ℃/12h), night time (18 ℃/12h), and illumination intensity (500 +/-20) mu mol.m.m-2·s-1Air relative humidity of 60-80 percent. Transplanting the seedlings into a nutrition pot when two leaves and one heart of the seedlings are needed, and selecting the cucumber seedlings with consistent growth vigor to carry out the following experimental treatment.
1.2.1 ABA and CaCl2Influence of single spraying on cold resistance of cucumber seedlings
The test adopts a method of foliage spraying to treat, the ABA solution is set to 3 levels, namely the concentration is respectively 15, 25 and 35mg/L (respectively set to S1, S2 and S3), CaCl2The solutions were set at 3 levels, i.e. concentrations of 500, 1000 and 1500mg/L respectively (S4, S5, S6 respectively), against spray water (CK). Clear water, ABA solution and CaCl2The solution is respectively and uniformly sprayed on the leaves of cucumber seedlings, and the spraying amount is 3 mL/plant. Spraying at 9 am every day, continuously spraying for 3d, continuously growing for 1d in a sunlight greenhouse, and placing in an artificial climate room for low temperature stress treatment, wherein the day and night temperature is set to be (5 +/-0.5) DEG C, and the light cycle is illumination 12 h/darkness 12 h. After 72h of treatment, the phenotype of each treatment is observed, and the cold injury index, MDA content, relative conductivity, SOD enzyme activity and CAT enzyme activity under different treatments are respectively determined.
1.2.2 ABA and CaCl2Influence of compound combination treatment on cold resistance of cucumber seedlings
The experiment adopts a method of foliage spraying to treat ABA and CaCl2The compound solution is set to 9 levels in total, namely 15mg/L ABA +500mg/L CaCl2、25mg/L ABA+500mg/L CaCl2、35mg/L ABA+500mg/L CaCl2、15mg/L ABA+1000mg/L CaCl2、25mg/L ABA+1000mg/L CaCl2、35mg/L ABA+1000mg/L CaCl2、15mg/L ABA+1500mg/L CaCl2、25mg/L ABA+1500mg/L CaCl2、35mg/L ABA+1500mg/L CaCl2(T1, T2, T3, T4, T5, T6, T7, T8, T9, respectively) with spray water as a Control (CK). The clear water and the compound solutions are respectively and uniformly sprayed on the leaves of the cucumbers, and the spraying amount is 3 mL/plant. Spraying at 9 am every day, continuously spraying for 3d, continuously growing for 1d in a sunlight greenhouse, and placing in an artificial climate room for low temperature stress treatment, wherein the day and night temperature is set to be (5 +/-0.5) DEG C, and the light cycle is illumination 12 h/darkness 12 h. After 72h of treatment, the phenotype of each treatment was observed and measured differentlyCold injury index, MDA content, relative conductivity, SOD enzyme activity and CAT enzyme activity under treatment.
1.3 measurement index and method
1.3.1 determination of Cold injury index
Grading the cold injury symptoms of the seedlings: grade 0 is no victim symptoms; the 1 st stage is that the leaves are slightly shrunken, the leaf margin of the 1 st or 2 nd leaf is yellow or slightly dehydrated, and the 3 rd leaf and the new leaf have no damage symptom; the 2-stage is the shrinkage of leaves, the 1 st leaf and the 2 nd leaf edge are seriously dehydrated, the 3 rd leaf edge is yellow or slightly dehydrated, and the heart leaves have no obvious damage symptoms; grade 3 is that dehydration spots appear in the middle of the 1 st and 2 nd leaves from the lower number, the 3 rd leaf margin is seriously dehydrated, and the heart leaves are slightly dehydrated; the 4-grade is that the dehydration spots in the middle parts of the 1 st leaf and the 2 nd leaf are connected into slices, the leaves are wilted, the dehydration spots begin to appear in the middle part of the 3 rd leaf, the heart leaf dehydration is obvious, but the heart leaf can still recover at normal temperature; grade 5 is that all leaves are severely water-loss and wilting, and seedlings can not recover at normal temperature. The chilling injury index was calculated as follows.
Cold injury index (1 × S1+2 × S2+3 × S3+4 × S4+5 × S5+0 × S0)/(number of treated strains × 5)
Wherein, S0-S5 are respectively the seedling number of 0-5 grades.
1.3.2 determination of relative conductivity
Washing fresh cucumber leaf sample with tap water, washing with distilled water for 2 times, punching the disc with a puncher, taking 0.5g of disc, placing into a large test tube, adding 20ml of distilled water, pumping for 3 times, each time for 20min, taking out after the first pumping, shaking for 3-4h at room temperature, shaking for multiple times, measuring the conductivity by using a thunder magnetic (DDSJ-318) conductivity meter S1, sealing in a boiling water bath for 10min, cooling (optionally soaking with cold water), balancing for 10min, measuring S2, and measuring the distilled water S0.
Calculating the formula: relative conductivity (%) ═ 100 × (S1-S0)/(S2-S0)
1.3.3 measurement of the Activity of malondialdehyde and antioxidant enzymes
The Malondialdehyde (MDA) content is determined by thiobarbituric acid (TBA) color development method, the superoxide dismutase (SOD) activity is determined by Nitrogen Blue Tetrazolium (NBT) method, and the Catalase (CAT) activity is determined by ultraviolet spectrophotometry.
1.4 data processing
Data were processed and plotted using Microsoft Excel 2010 and GraphPad Prism 6 software; SPSS 17.0 software performed one-way analysis of variance on the data and multiple comparisons of significance differences (P <0.05) using Duncan test.
2 results and analysis
2.1 ABA and CaCl2Influence of single spraying on phenotype of cucumber seedlings under low-temperature stress
As can be seen from the phenotype in FIG. 1, when the leaves of the cucumber are stressed for 72 hours at low temperature, the water loss of the leaves (the second leaves from the top) of the cucumber after CK treatment is serious, and the wilting and yellowing phenomenon appears at the edges. Compared with CK, the leaves treated by S2 and S5 have no obvious water loss wilting phenomenon, and other treatments have wilting and yellowing phenomena with different degrees.
2.2 ABA and CaCl2Influence of single spraying on cold damage index and MDA content of cucumber seedlings under low-temperature stress
The cold injury index can reflect the damage degree of the cucumber seedlings under low-temperature stress, and ABA solutions with the concentrations of 15mg/L, 25mg/L and 35mg/L are respectively used for spraying and treating the leaves of the cucumber seedlings. The results show that: when treated at low temperature for 72h, cucumber seedlings were damaged to varying degrees. Compared with CK, the cold injury index after S1, S2 and S3 treatments is obviously reduced by 17.64 percent, 67.64 percent and 47.06 percent (P)<0.05) (a in fig. 2); at the same time, 500mg/L, 1000mg/L and 1500mg/L CaCl were used respectively2Spraying treatment is carried out on leaves of cucumber seedlings, and the result shows that: compared with CK, the cold injury index after the treatment of S4, S5 and S6 is obviously reduced by 20.59 percent, 32.35 percent and 14.70 percent (P)<0.05) (B in fig. 2). Shows that exogenous spraying proper concentration ABA and CaCl2The solution can reduce the cold damage index of cucumber seedlings and improve the low temperature resistance of the cucumber seedlings.
MDA is the final product of membrane lipid peroxidation, and the cell membrane is damaged due to the accumulation of MDA, so the MDA content can be used to indicate the degree of membrane lipid peroxidation. The occurrence mechanism of cold injury is considered to be the most accepted theory of membrane lipid phase transition, so MDA is often used as an index for representing the degree of cold injury. And measuring the MDA content of the cucumber leaves after low-temperature treatment for 72 h. Compared with CKAfter treatment with S1, S2 and S3, the MDA content is significantly reduced, namely, the MDA content is reduced by 7.68%, 20.57% and 12.48% (P)<0.05) (C in fig. 2); compared with CK, the MDA content after S4 and S6 treatments has no significant difference, and the MDA content after S5 treatment is significantly reduced by 22.99 percent (P5 treatment)<0.05) (D in fig. 2). Shows that exogenous spraying proper concentration ABA and CaCl2The solution can effectively relieve the accumulation of the peroxidation products of the membrane lipid, thereby improving the cold resistance of the cucumber seedlings.
In conclusion, of all ABA treatments, the S2 treatment (25mg/L) has the best effect of improving the low-temperature resistance of cucumber seedlings; all of CaCl2Among the treatments, the S5 treatment (1000mg/L) was most effective in improving the cold resistance of cucumber seedlings.
2.3 ABA and CaCl2Influence of single spraying on relative conductivity of cucumber seedling leaves under low-temperature stress
And respectively using ABA solutions with the concentrations of 15mg/L, 25mg/L and 35mg/L to spray the leaves of the cucumber seedlings. The results show that: when the cucumber leaves are treated at the low temperature for 72h, the relative conductivity of the cucumber leaves after the treatment of S1, S2 and S3 is remarkably reduced compared with that of CK, and is respectively reduced by 22.57%, 35.90% and 29.43% (P <0.05), wherein the treatment effect is the best at S2 (A in figure 3); meanwhile, the relative conductivity of the cucumber leaves after the S4 and S5 treatments is remarkably reduced compared with that of CK by 21.28% and 26.58% (P <0.05), respectively, and the relative conductivity of the cucumber leaves after the S6 treatment is not remarkably different from that of CK (B in figure 3), so that the treatment effect is optimal with the S5 treatment.
In conclusion, of all ABA treatments, the S2 treatment (25mg/L) has the best effect of improving the low-temperature resistance of cucumber seedlings; all of CaCl2In the treatment, the S5 treatment (1000mg/L) has the best effect of improving the cold resistance of the cucumber seedlings, can reduce the exosmosis of electrolytes and improve the low temperature resistance of the cucumber seedlings.
2.4 ABA and CaCl2Influence of single spraying on antioxidant enzyme activity of cucumber seedling leaves under low-temperature stress
The antioxidant enzyme can scavenge free radicals and protect cell membrane systems, protecting cells from toxic effects. The activity of the enzyme can directly reflect the adaptability of cells to the adverse environment and is positively correlated with the resistance of plants. Respectively using a concentration ofSpraying 15mg/L, 25mg/L and 35mg/L ABA solution on leaves of cucumber seedlings. The results show that: when treated at low temperature for 72h, SOD enzyme activity was significantly increased after treatment with S1, S2, and S3, 12.61%, 26.97%, and 17.22% (A in FIG. 4) (P), respectively, as compared to CK<0.05), CAT enzyme activity was significantly increased by 23.08%, 48.31% and 22.47% (C in FIG. 4) (P), respectively<0.05); at the same time, 500mg/L, 1000mg/L and 1500mg/L CaCl were used respectively2Spraying treatment is carried out on leaves of cucumber seedlings, and the result shows that: compared with CK, SOD enzyme activity is obviously increased after S4 and S5 treatment, and is respectively increased by 13.73% and 20.06% (P)<0.05), no significant difference in SOD enzyme activity after S6 treatment (B in fig. 4); compared with CK, CAT enzyme activity is remarkably improved after S4 and S5 treatment, and is respectively increased by 27.24 percent and 37.71 percent (P)<0.05), no significant difference in CAT enzyme activity after S6 treatment (D in fig. 4). Explanation of externally applied proper concentrations of ABA and CaCl2The solution can effectively improve the activity of antioxidant enzyme, thereby improving the cold resistance of cucumber seedlings.
In conclusion, of all ABA treatments, the S2 treatment (25mg/L) has the best effect of improving the low-temperature resistance of cucumber seedlings; all of CaCl2Among the treatments, the S5 treatment (1000mg/L) was most effective in improving the cold resistance of cucumber seedlings.
2.5 ABA and CaCl2Influence of compound combined treatment on phenotype of cucumber seedlings under low-temperature stress
As shown in FIG. 5, when the leaves of the CK-treated cucumber seedlings (the first, second and third leaves) are severely wilted and curled at 72h of low temperature stress, partial leaves have yellowing phenomenon, other treatments have reduced symptoms compared with CK, the marginal wilting curling of partial leaves under the treatment of T1, T2, T4, T5 and T6, and the leaves treated by T7, T8 and T9 are mainly characterized in that the leaves are severely yellowed and partial leaves have wilting, probably due to CaCl applied to the treatment2Higher concentrations lead to yellowing of the leaves. The treatment effect of T3 is optimal, and the leaves have no obvious water loss and wilting phenomenon.
2.6 ABA and CaCl2Influence of compound combination treatment on cucumber seedling cold damage index and MDA content under low temperature stress
As can be seen from FIG. 6, the cucumber leaves were sprayed with the different concentrations of the compounded solutions. The results show that: when the cucumber seedling is stressed at low temperature for 72h, compared with CK, the cold injury index and MDA content in each treatment are obviously reduced, and the damage of the low temperature to the cucumber seedling is effectively relieved. Among them, the cold injury index and MDA content of T3 treatment were significantly reduced by 74.36% and 41.70%, respectively, compared to CK (P < 0.05). In conclusion, in 9 treatments, the cold damage index and the MDA content of the leaves are analyzed and compared together, and the cold resistance of the cucumber seedlings is improved better than that of the control by each treatment. The T3 treatment was most effective in improving the cold tolerance of cucumber seedlings.
2.7 ABA and CaCl2Influence of compound combination treatment on relative conductivity of cucumber seedling leaves under low-temperature stress
As can be seen from FIG. 7, the cucumber leaves were sprayed with the different concentrations of the compounded solutions. The results show that: when the cucumber seedling is stressed at low temperature for 72h, compared with CK, the relative conductivity of each treatment is obviously reduced, the exosmosis of electrolyte is effectively reduced, and the damage degree of the cucumber seedling is relieved to a certain extent. The relative conductivity of the T3 treatment was significantly reduced by 46.44% (P <0.05) compared to CK, and in conclusion, the T3 treatment was the best to improve the cold resistance of cucumber seedlings.
2.8 ABA and CaCl2Influence of compound combination treatment on cucumber seedling leaf antioxidase activity under low-temperature stress
When plants are subjected to low temperature stress, the accumulation of reactive oxygen species in large quantities results in membrane peroxidation, thereby disrupting the structure of the cell membrane. Plants must remove excessive active oxygen in time through an antioxidant system in vivo, and antioxidant enzymes such as superoxide dismutase (SOD), Catalase (CAT) and the like have important effects on preventing active oxygen damage and maintaining the integrity of a cell membrane structure. As can be seen from a in fig. 8, each treatment significantly improved SOD enzyme activity of cucumber seedling leaves, and T3 treatment significantly improved SOD enzyme activity compared to CK and other treatments (P < 0.05). Compared with CK, the SOD enzyme activity of T3 treated SOD is improved by 70.36% (P <0.05) to eliminate excessive active oxygen and relieve the damage of low temperature to seedling. Meanwhile, the CAT enzyme activity of the cucumber seedling leaves is improved (P is less than 0.05) through each treatment, a cell membrane system is protected to a certain extent, and low-temperature damage is relieved. The CAT enzyme activity of the T3 treatment was significantly higher than that of the CK and other treatments. Compared to CK, T3 treated CAT enzyme activity was significantly increased by 54.32% (P <0.05) (B in fig. 8). In conclusion, the T3 treatment obviously improves the antioxidant enzyme activity of leaves of cucumber seedlings, thereby improving the cold resistance of the cucumber seedlings.
In conclusion, the optimal concentration of the ABA treated alone is 25mg/L, CaCl2The optimum concentration of the single treatment is 1000mg/L, and the optimum concentration of the compound combination treatment is 35mg/L ABA +500mg/L CaCl2. Under low temperature stress, the components are compounded and combined to be processed (35mg/L ABA +500mg/L CaCl)2) The improvement of the antioxidant enzyme activity of cucumber seedling leaves is better than that of ABA (25mg/L) alone or CaCl alone2The cold damage index, MDA content and relative conductivity of the (1000mg/L) treated and compounded combined treated products are all lower than that of ABA (25mg/L) alone or CaCl alone2(1000 mg/L).
Therefore, 25mg/L ABA or 1000mg/L CaCl are sprayed separately2Can relieve the damage of low temperature to cucumber seedlings, but is treated by combination of (35mg/L ABA +500mg/L CaCl)2) The overall effect is better.

Claims (10)

1. A composition for improving cold resistance of cucumber seedlings comprises ABA and CaCl as active ingredients2。
2. The composition as claimed in claim 1, wherein the active ingredients consist of ABA and CaCl2And (4) forming.
3. The composition of claim 1 or 2, wherein ABA and CaCl are present in the composition2The weight ratio of (A) to (B) is 6.5-7.5: 100; preferably, ABA and CaCl in the composition2In a weight ratio of 7: 100.
4. The composition according to any one of claims 1 to 3, wherein the composition is a solution.
5. The composition of claim 1 or 2, wherein the composition is ABA and CaCl2The mixed solution of (1), wherein each liter of ABA and CaCl2The mixed solution of (A) contains 32.5-37.5mg of ABA and 500mg of CaCl2(ii) a Preferably ABA and CaCl per liter2The mixed solution of (A) contained 35mg of ABA and 500mg of CaCl2。
6. The composition of any one of claims 1 to 3, wherein the composition is an ABA solution and CaCl2A solution; wherein the concentration of the ABA solution is 15-35 mg/L; the CaCl is2The solution concentration is 500-1500 mg/L;
preferably, the concentration of the ABA solution is 25-35 mg/L; the CaCl is2The solution concentration is 1000-1500 mg/L;
more preferably, the concentration of the ABA solution is 35 mg/L; the CaCl is2The concentration of the solution was 500 mg/L.
7. Use of a composition according to any one of claims 1 to 6 for improving the cold resistance of cucumber seedlings.
8. Use of a composition according to any one of claims 1 to 6 for any one or more of increasing antioxidase activity of leaves of cucumber seedlings, decreasing cold damage index of cucumber seedlings, decreasing MDA content of leaves, decreasing relative electrical conductivity of leaves.
9. A method for improving the cold resistance of cucumber seedlings, which comprises the following steps: providing a composition according to any one of claims 1 to 6 in the form of a solution; the composition is sprayed on the leaf surfaces of cucumber seedlings.
10. The method according to claim 9, wherein the cucumber variety is 'Zhongnong 26'; and/or the presence of a gas in the gas,
the cucumber seedling refers to a seedling with two leaves and one heart; and/or the presence of a gas in the gas,
spraying the composition in the morning, preferably continuously for 3-5 days, during the seedling stage of the cucumber; and/or the presence of a gas in the gas,
the spraying amount of the composition is 2-6 mL/plant, and preferably 3 mL/plant.
CN202111108926.9A 2021-09-22 2021-09-22 Composition for improving cold resistance of cucumber seedlings and application thereof Pending CN113693068A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008094587A1 (en) * 2007-01-31 2008-08-07 Valent Biosciences Corporation Enhanced abscisic acid and fertilizer performance

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008094587A1 (en) * 2007-01-31 2008-08-07 Valent Biosciences Corporation Enhanced abscisic acid and fertilizer performance

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨楠: "钙对水杨酸与脱落酸诱导的黄瓜幼苗耐冷性的影响", 《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑(月刊)》 *

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