CN113670919A - Micro-bubble visualization device and visualization method for micro-fluidic chip in temperature changing process - Google Patents
Micro-bubble visualization device and visualization method for micro-fluidic chip in temperature changing process Download PDFInfo
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- CN113670919A CN113670919A CN202110869788.XA CN202110869788A CN113670919A CN 113670919 A CN113670919 A CN 113670919A CN 202110869788 A CN202110869788 A CN 202110869788A CN 113670919 A CN113670919 A CN 113670919A
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000012800 visualization Methods 0.000 title claims abstract description 10
- 238000007794 visualization technique Methods 0.000 title claims abstract description 9
- 238000005057 refrigeration Methods 0.000 claims abstract description 32
- 238000010438 heat treatment Methods 0.000 claims abstract description 22
- 230000033001 locomotion Effects 0.000 claims abstract description 15
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 229920001296 polysiloxane Polymers 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8405—Application to two-phase or mixed materials, e.g. gas dissolved in liquids
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Abstract
The invention discloses a micro-bubble movement visualization device and a visualization method for a micro-fluidic chip in a temperature changing process; the micro-fluidic chip, the TEC refrigeration piece and the thermocouple are simultaneously placed in the chip box main body, the TEC refrigeration piece and the thermocouple are electrically connected with an external temperature controller, and a window for observing the micro-fluidic chip is formed in the chip box cover part; the light source emits light beams, the light beams are incident to the beam splitter prism to be reflected after passing through the collimating lens, reflected light beams of the beam splitter prism are irradiated onto the microfluidic chip in the cover window of the chip box after passing through the microscope objective, and the light beams reflected by the microfluidic chip are received by the CCD camera; the computer is electrically connected with the CCD camera and receives and displays images shot by the CCD camera; the heating observation module is integrated into the chip box, so that the chip box has the characteristics of small volume and high flexibility; the chip box is vertically arranged and matched with the corresponding light path, so that the micro-bubble micro-fluidic chip can micro-shoot the movement process of micro-bubbles in the micro-fluidic chip under the influence of buoyancy.
Description
Technical Field
The invention relates to a micro-bubble visualization device for a micro-fluidic chip, in particular to a micro-bubble visualization device and a visualization method for the micro-fluidic chip in a temperature changing process.
Background
In order to understand the movement mode of the microbubbles under the influence of buoyancy in the temperature changing process, an experimental device which can heat the microfluidic chip in a vertical state and shoot in real time is needed, and the conventional microfluidic chip heating device has the problems that the volume is large, the microfluidic chip cannot be effectively fixed and the like, and cannot be applied to microscopic shooting under the action of buoyancy in the vertical direction.
Disclosure of Invention
In order to solve the problems existing in the background technology, the invention aims to provide a device and a method for visualizing micro-bubbles of a micro-fluidic chip in a temperature changing process, so as to achieve the purpose of observing the movement mode of the micro-bubbles of the micro-fluidic chip under the influence of buoyancy in the temperature changing process.
In order to achieve the purpose, the invention provides the following technical scheme:
a micro-bubble visualization device of a micro-fluidic chip in a temperature changing process comprises the following steps:
the device comprises a heating module, an observation module and a display module, wherein the observation module is arranged beside the heating module and is electrically connected with the display module; the heating module comprises a chip box, a temperature controller and a power supply, the chip box is divided into a chip box main body and a chip box cover part, the chip box cover part is fixedly covered on the upper part of the chip box main body, a microfluidic chip, a TEC refrigeration piece and a thermocouple are simultaneously placed in the chip box main body, the upper part of the chip box main body is a boss, the middle part of the boss is provided with a rectangular groove, the top surface of the boss is provided with a T-shaped groove, the top surface of the rectangular groove is communicated with the bottom surface of the T-shaped groove, one end of the rectangular groove is communicated with the side surface of the boss, the TEC refrigeration piece is placed into the rectangular groove through the rectangular groove opening and is tightly attached, a hole penetrating through the chip box main body is arranged below the rectangular groove, the TEC refrigeration piece is placed above the hole, the refrigeration TEC piece is fixed into the rectangular groove through the lower hole and is directly contacted with outside air, heat release and absorption are facilitated, the microfluidic chip and the thermocouple are simultaneously placed in the T-shaped groove, and the microfluidic chip and the thermocouple are uniformly distributed on the TEC refrigeration piece, the TEC refrigeration piece and the thermocouple are electrically connected with an external temperature controller, the temperature controller is connected with a power supply, and a window for observing the microfluidic chip is formed in the chip box cover part; the observation module comprises a light source, a collimating lens, a beam splitter prism, a microscope objective and a CCD camera, wherein the light source emits light beams, the light beams are incident to the beam splitter prism to be reflected after passing through the collimating lens, reflected light beams of the beam splitter prism are irradiated onto a microfluidic chip in a window of the cover part of the chip box after passing through the microscope objective, the light beams reflected by the microfluidic chip are incident to the CCD camera after being transmitted by the microscope objective and the beam splitter prism in sequence to form a light path of the observation module, and the microscope objective is opposite to the window of the cover part of the chip box; the display module comprises a computer, and the computer is electrically connected with the CCD camera and receives and displays images shot by the CCD camera.
The four corners department of chip box main part and chip box lid set up the hole of taking the screw thread, use the screw to pass the hole of taking the screw thread in chip box main part and the chip box lid and then make up fixedly between with chip box main part and the chip box lid.
The side part of the chip box main body is clamped in the clamp, and the chip box is vertically placed by taking the clamp as the bottom.
The temperature controller is electrically connected with the thermocouple, the computer, the power supply and the refrigerating piece, the thermocouple feeds back real-time temperature, the output power of the temperature controller is changed by setting parameters or adjusting the voltage of the power supply through the computer, and then the temperature of the TEC refrigerating piece is adjusted, and further the temperature of the microfluidic chip is adjusted.
The computer receives images of micro-bubbles in the micro-fluidic chip shot by the CCD camera in real time and displays the images in real time through a display screen of the computer.
The beam splitting prism is 50: 50 half-transmitting and half-reflecting cubic prism.
Secondly, a micro-bubble visualization method of the micro-fluidic chip in the temperature changing process:
the method comprises the following steps: connecting a temperature controller to a power supply, separately checking the temperature controller and a TEC refrigerating sheet to check whether the temperature controller and the TEC refrigerating sheet can work normally, attaching a thermocouple to the TEC refrigerating sheet after the normal work is confirmed, and connecting the thermocouple and the TEC refrigerating sheet to the temperature controller; unscrewing screws for fixing four corners of the chip box cover part, moving the chip box cover part away, loading the TEC refrigeration piece into a rectangular groove of a boss at the upper part of the chip box main body, placing the microfluidic chip on the TEC refrigeration piece, and installing the chip box cover part back to the upper part of the chip box main body after the situation that no errors exist is confirmed; clamping the chip box by using a clamp, and vertically placing the clamp and the whole chip box so that a window of a cover part of the chip box is opposite to the microscope objective; turning on a light source switch, adjusting the focal length of the microscope objective through a light path formed by the observation module, and shooting images of micro bubbles in the microfluidic chip by using a CCD (charge coupled device) camera and transmitting the images into a computer until clear images can be seen on a display screen of the computer; starting a temperature controller, and setting parameters such as heating temperature, heating rate, heat preservation time, cooling rate and the like; recording and storing images or videos of the movement of the microbubbles in the microfluidic chip in the heating process by using a computer, and monitoring the temperature in real time; and after the observation is finished, closing the light source switch, waiting for the TEC refrigerating piece to be completely cooled, then closing the temperature controller, opening the chip box, taking out the microfluidic chip, cleaning, and finally resetting the chip box.
The upper surface of the TEC refrigeration piece is coated with a layer of heat-conducting silicone grease, so that the microfluidic chip is heated more uniformly.
The micro-fluidic chip is externally connected with an injection pump or gas cylinder equipment, and the injection pump or gas cylinder equipment injects the interior of the micro-fluidic chip to realize real-time regulation and control of micro-bubbles in the micro-fluidic chip.
Compared with the prior art, the invention has the beneficial effects that:
the heating observation module is integrated into the chip box, so that the volume is greatly reduced, and the chip box can be normally observed by using a microscope when being horizontally placed; the micro-bubble micro-fluidic chip can be vertically placed after being clamped by the clamp, and the micro-bubble micro-fluidic chip can be used for micro-shooting the vertical movement process of micro-bubbles in the micro-fluidic chip under the action of buoyancy by matching with a corresponding light path.
Drawings
FIG. 1 is a schematic diagram of the apparatus of the present invention;
FIG. 2 is a schematic diagram of a visualization method after the device of the present invention is connected to a light path;
in the figure: 1. chip box, 101, microfluidic chip, 102, TEC refrigeration piece, 103, thermocouple, 104, chip box main body, 105, chip box cover, 2, clamp, 3, temperature controller, 4, light source, 5, collimating lens, 6, beam splitting prism, 7, microscope objective, 8, CCD camera, 9, computer, 10 and power supply.
Detailed Description
The present invention now will be described more fully hereinafter with reference to the accompanying drawings.
As shown in FIG. 1, a chip cartridge 1 is provided, wherein a chip cartridge 1 is held by a holder 2; the chip box 1 is divided into a chip box main body 104 and a chip box cover part 105, the chip box cover part 105 is fixedly covered on the upper part of the chip box main body 104, the microfluidic chip 101, the TEC refrigeration piece 102 and the thermocouple 103 are simultaneously placed in the chip box main body 104, the side part of the chip box main body 104 is clamped in the clamp 2, and the chip box 1 is vertically placed by taking the clamp 2 as the bottom; the upper part of the chip box main body 104 is provided with a boss, the size of the boss of the chip box main body 104 is the same as that of the chip box cover part 105, holes with threads are arranged at the four corners of the chip box main body 104 and the chip box cover part 105, and screws are used for penetrating the holes with threads in the chip box main body 104 and the chip box cover part 105 so as to combine and fix the chip box main body 104 and the chip box cover part 105; the middle part of the lug boss is provided with a rectangular groove, the top surface of the lug boss is provided with a T-shaped groove, the top surface of the rectangular groove is communicated with the bottom surface of the T-shaped groove, one end of the rectangular groove is communicated with the side surface of the lug boss, the size of the rectangular groove is the same as that of the TEC refrigeration piece 102 and is used for placing the TEC refrigeration piece 102,
a hole penetrating through the chip box main body 104 is arranged below the rectangular groove, the size of the hole is smaller than that of the TEC refrigeration piece 102, the TEC refrigeration piece 102 is placed into the rectangular groove through the rectangular groove and is tightly attached to the rectangular groove, the TEC refrigeration piece 102 is placed on the hole, the TEC refrigeration piece 102 is fixed in the rectangular groove through the lower hole and is in direct contact with the outside air, heat release and heat absorption are facilitated, the thermocouple 103 and the microfluidic chip 101 are uniformly distributed on the TEC refrigeration piece 102, a T-shaped groove is formed in a boss of the chip box main body 104 above the TEC refrigeration piece 102 and penetrates through the boss downwards, the size of the T-shaped groove is the same as that of the microfluidic chip 101 and the thermocouple 103, the bottom of the T-shaped groove is flush with the top of the rectangular groove below, the microfluidic chip 101 and the thermocouple 103 are simultaneously placed in the T-shaped groove, the upper part of the T-shaped groove is used for placing the microfluidic chip 101 to be heated and observed, and the thermocouple 103 is fixedly contacted with the top surface of one side of the TEC refrigeration piece 102 close to the rectangular groove, when the TEC refrigeration piece 102 is placed in the rectangular groove, the thermocouple 103 at the upper part can pass through the lower part of the T-shaped groove; the upper part of the microfluidic chip 101 is an observation part, and the chip cartridge cover 105 is provided with a window for observing the microfluidic chip 101, so that the observation part of the microfluidic chip 101 can be exposed.
As shown in fig. 1 and 2, the heating module of the whole apparatus includes a chip box 1, a temperature controller 3 and a power supply 10, the temperature controller 3 is electrically connected to a thermocouple 103, the power supply 10 and a TEC refrigeration plate 102, the thermocouple 103 feeds back real-time temperature, and the temperature controller 3 adjusts the temperature of the TEC refrigeration plate 102, thereby adjusting the temperature of the microfluidic chip 101.
As shown in fig. 2, the whole device comprises a heating module, an observation module and a display module, wherein the observation module is arranged beside the heating module, and the observation module is electrically connected with the display module; the observation module includes light source 4, collimating lens 5, beam splitter prism 6, microscope objective 7 and CCD camera 8, the center of observation module device all is in the coplanar, light source 4, collimating lens 5, beam splitter prism 6 is located the collinear, microscope objective 7 and CCD camera 8 are located the both sides of beam splitter prism 6 respectively, light source 4, collimating lens 5, the straight line and microscope objective 7 that beam splitter prism 6 formed, CCD camera 8, the straight line quadrature that beam splitter prism 6 formed, light source 4 sends the light beam, incide 50 after collimating lens 5 transmits: 50, a reflected beam of the beam splitter prism 6 irradiates the microfluidic chip 101 in a window of a chip box cover part 105 after passing through a microscope objective 7, the light beam reflected by the microfluidic chip 101 is transmitted by the microscope objective 7 and the beam splitter prism 6 in sequence and then is incident on a CCD camera 8 to form a light path of an observation module, the microscope objective 7 is right opposite to the window of the chip box cover part 105 and used for observing the microfluidic chip 101, and an image of the microfluidic chip 101 is acquired by the CCD camera 8; the display module comprises a computer 9, the computer 9 is electrically connected with the temperature controller 3 and the CCD camera 8, parameters are set or the voltage of the power supply 10 is adjusted through the computer 9, the output power of the temperature controller 3 is changed, the temperature of the TEC refrigerating plate 102 can be adjusted, meanwhile, the computer 9 can receive and display images of micro bubbles of the micro-fluidic chip 101 shot by the CCD camera 8 in real time, and the images are displayed through a display screen of the computer 9 in real time.
The method comprises the following steps: the temperature controller 3 is connected to the power supply 10, a layer of heat-conducting silicone grease is coated on the upper surface of the TEC refrigerating plate 102, so that the microfluidic chip 101 is heated more uniformly, the temperature controller 3 and the TEC refrigerating plate 102 are checked independently to check whether the normal work can be carried out or not, the thermocouple 103 is attached to the TEC refrigerating plate 102 after the normal work is confirmed, and the thermocouple 103 and the TEC refrigerating plate 102 are connected to the temperature controller 3; unscrewing screws for fixing four corners of the chip box cover part 105, moving the chip box cover part 105 away, installing the TEC refrigeration piece 102 into a rectangular groove of a boss at the upper part of the chip box main body 104, placing the microfluidic chip 101 on the TEC refrigeration piece 102, externally connecting the microfluidic chip 101 with an injection pump or gas cylinder device, injecting the micro-fluidic chip 101 by the injection pump or the gas cylinder device, realizing real-time regulation and control of micro-bubbles in the microfluidic chip 101, and installing the chip box cover part 105 back to the upper part of the chip box main body 104 after confirming no error; clamping the chip box 1 by using the clamp 2, and vertically placing the whole of the clamp 2 and the chip box 1 so that the window of the chip box cover part 105 is opposite to the microscope objective lens 7; a switch of a light source 4 is turned on, the focal length of a microscope objective lens 7 is adjusted through a light path formed by an observation module, and a CCD camera 8 is used for shooting images of micro bubbles in the microfluidic chip 101 and transmitting the images into a computer 9 until clear images can be seen on a display screen of the computer 9; starting the temperature controller 3, and setting parameters such as heating temperature, heating rate, heat preservation time, cooling rate and the like; recording and storing images or videos of the movement of the microbubbles in the microfluidic chip 101 in the heating process by using the computer 9, and monitoring the temperature in real time; and after the observation is finished, the switch of the light source 4 is turned off, the TEC refrigerating sheet 102 is waited to be completely cooled, then the temperature controller 3 is turned off, the chip box 1 is opened, the microfluidic chip 101 is taken out and cleaned, and finally the chip box 1 is reset.
According to the micro-bubble visualization device for the micro-fluidic chip in the temperature changing process, the heating part is integrated into the chip box, so that the size is greatly reduced, the micro-bubble visualization device can be normally observed by using a microscope when being horizontally placed, can be vertically placed after being clamped by using a clamp, and is matched with a corresponding light path, so that the micro-bubble visualization device can be used for microscopically shooting the vertical motion process of micro-bubbles in the micro-fluidic chip under the action of buoyancy.
Claims (9)
1. The utility model provides a alternating temperature in-process micro-fluidic chip microbubble motion visualization device which characterized in that: the device comprises a heating module, an observation module and a display module, wherein the observation module is arranged beside the heating module and is electrically connected with the display module; the heating module comprises a chip box (1), a temperature controller (3) and a power supply (10), the chip box (1) is divided into a chip box main body (104) and a chip box cover part (105), the chip box cover part (105) is fixedly covered on the upper part of the chip box main body (104), a micro-fluidic chip (101), a TEC refrigerating sheet (102) and a thermocouple (103) are simultaneously placed in the chip box main body (104), a boss is arranged on the upper part of the chip box main body (104), a rectangular groove is formed in the middle of the boss, a T-shaped groove is formed in the top surface of the boss, the top surface of the rectangular groove is communicated with the bottom surface of the T-shaped groove, one end of the rectangular groove is communicated with the side surface of the boss and used for placing the TEC refrigerating sheet (102), a hole penetrating through the chip box main body (104) is formed below the rectangular groove, the micro-fluidic refrigerating sheet (102) is placed on the hole, the micro-fluidic chip (101) and the thermocouple (103) are simultaneously placed in the T-shaped groove, the micro-fluidic chip (101) and the thermocouple (103) are uniformly distributed on the TEC refrigerating sheet (102), the TEC refrigeration piece (102) and the thermocouple (103) are electrically connected with an external temperature controller (3), the temperature controller (3) is connected with a power supply (10), and a window for observing the microfluidic chip (101) is formed in the chip box cover part (105); the observation module comprises a light source (4), a collimating lens (5), a beam splitter prism (6), a microscope objective (7) and a CCD camera (8), wherein the light source (4) emits light beams which are incident to the beam splitter prism (6) to be reflected after passing through the collimating lens (5), reflected light beams of the beam splitter prism (6) are irradiated to a microfluidic chip (101) in a window of a chip box cover part (105) after passing through the microscope objective (7), the light beams reflected by the microfluidic chip (101) are incident to the CCD camera (8) after being transmitted by the microscope objective (7) and the beam splitter prism (6) in sequence to form a light path of the observation module, and the microscope objective (7) is opposite to the window of the chip box cover part (105); the display module comprises a computer (9), wherein the computer (9) is electrically connected with the CCD camera (8) and receives and displays images shot by the CCD camera (8).
2. The device for visualizing micro-bubble movement of a microfluidic chip in a temperature varying process according to claim 1, wherein: the four corners of the chip box main body (104) and the chip box cover part (105) are provided with holes with threads, and screws are used for penetrating the holes with threads in the chip box main body (104) and the chip box cover part (105) so as to combine and fix the chip box main body (104) and the chip box cover part (105).
3. The device for visualizing micro-bubble movement of a microfluidic chip in a temperature varying process according to claim 1, wherein: the side part of the chip box main body (104) is clamped in the clamp (2), and the chip box (1) is vertically placed by taking the clamp (2) as the bottom.
4. The device for visualizing micro-bubble movement of a microfluidic chip in a temperature varying process according to claim 1, wherein: the temperature controller (3) is electrically connected with the thermocouple (103), the computer (9), the power supply (10) and the TEC refrigeration piece (102).
5. The device for visualizing micro-bubble movement of a microfluidic chip in a temperature varying process according to claim 1, wherein: the computer (9) receives images of micro-bubbles in the micro-fluidic chip (101) shot by the CCD camera (8) in real time, and displays the images in real time through a display screen of the computer (9).
6. The device for visualizing micro-bubble movement of a microfluidic chip in a temperature varying process according to claim 1, wherein: the beam splitter prism (6) is 50: 50 half-transmitting and half-reflecting cubic prism.
7. Visualization method applied to the device of claim 1, characterized in that: the method comprises the following steps:
step 1): the temperature controller (3) is connected to a power supply (10), the temperature controller (3) and the TEC refrigerating piece (102) are independently checked to check whether the temperature controller and the TEC refrigerating piece can work normally or not, after the normal work is confirmed, the thermocouple (103) is attached to the TEC refrigerating piece (102), and the thermocouple (103) and the TEC refrigerating piece (102) are connected to the temperature controller (3);
step 2): the TEC refrigeration piece (102) is arranged in the chip box main body (104), the microfluidic chip (101) is placed on the TEC refrigeration piece (102), and the chip box cover part (105) is arranged back to the upper part of the chip box main body (104) after the situation that no error exists is confirmed;
step 3): clamping the chip box (1) by using the clamp (2), and vertically placing the clamp (2) and the whole chip box (1) so that the window of the chip box cover part (105) is opposite to the microscope objective (7);
step 4): a switch of a light source (4) is turned on, the focal length of a microscope objective (7) is adjusted through a light path formed by an observation module, a CCD camera (8) is used for shooting images of micro bubbles in the micro-fluidic chip (101) and transmitting the images into a computer (9) until clear images can be seen on a display screen of the computer (9);
step 5): starting the temperature controller (3), and setting parameters such as heating temperature, heating rate, heat preservation time, cooling rate and the like;
step 6): recording and storing images or videos of micro-bubble movement in the micro-fluidic chip (101) in the heating process by using a computer (9), and monitoring the temperature in real time;
step 7): and after the observation is finished, the switch of the light source (4) is turned off, the TEC refrigerating piece (102) is waited to be completely cooled, then the temperature controller (3) is turned off, and the chip box (1) is turned on to take out the microfluidic chip (101) and clean the microfluidic chip.
8. A visualization method as recited in claim 7, wherein: the upper surface of the TEC refrigeration piece (102) is coated with a layer of heat-conducting silicone grease.
9. A visualization method as recited in claim 7, wherein: the micro-fluidic chip (101) is externally connected with an injection pump or gas cylinder device, and the injection pump or gas cylinder device injects the interior of the micro-fluidic chip (101) to realize real-time regulation and control of micro-bubbles in the micro-fluidic chip (101).
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| CN202110869788.XA CN113670919A (en) | 2021-07-30 | 2021-07-30 | Micro-bubble visualization device and visualization method for micro-fluidic chip in temperature changing process |
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| CN202110869788.XA CN113670919A (en) | 2021-07-30 | 2021-07-30 | Micro-bubble visualization device and visualization method for micro-fluidic chip in temperature changing process |
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| CN106770288A (en) * | 2016-12-09 | 2017-05-31 | 江苏大学 | A kind of micro- Polaroid system and method for interference with common path phase |
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| JP2020204617A (en) * | 2020-09-02 | 2020-12-24 | 大日本印刷株式会社 | Microorganism contaminant detection device |
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