CN113670871A - Online detection method for concentration of adenosine triphosphate of microorganisms in air - Google Patents
Online detection method for concentration of adenosine triphosphate of microorganisms in air Download PDFInfo
- Publication number
- CN113670871A CN113670871A CN202110945356.2A CN202110945356A CN113670871A CN 113670871 A CN113670871 A CN 113670871A CN 202110945356 A CN202110945356 A CN 202110945356A CN 113670871 A CN113670871 A CN 113670871A
- Authority
- CN
- China
- Prior art keywords
- air
- microorganisms
- concentration
- detector
- adenosine triphosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 244000005700 microbiome Species 0.000 title claims abstract description 42
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 title claims abstract description 32
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 43
- 238000012360 testing method Methods 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 34
- 230000008569 process Effects 0.000 claims abstract description 19
- 238000005070 sampling Methods 0.000 claims abstract description 16
- 230000000087 stabilizing effect Effects 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 12
- 238000002189 fluorescence spectrum Methods 0.000 claims description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- -1 polytetrafluoroethylene Polymers 0.000 claims description 10
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 9
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 239000013307 optical fiber Substances 0.000 claims description 5
- 239000002912 waste gas Substances 0.000 claims description 5
- 238000009833 condensation Methods 0.000 claims description 2
- 230000005494 condensation Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 241000700605 Viruses Species 0.000 abstract description 6
- 239000000443 aerosol Substances 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 5
- 241000282414 Homo sapiens Species 0.000 abstract description 5
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 230000009471 action Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000428 dust Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 235000021062 nutrient metabolism Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to the technical field of air environment detection, in particular to an on-line detection method for the concentration of adenosine triphosphate of microorganisms in air, which comprises the following steps: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device, a test agent is injected into a rotational flow sampler, microorganisms in the air are absorbed by the test agent in the rotational flow process, and a bubble remover is connected behind the rotational flow sampler; the method for detecting the concentration of the adenosine triphosphate of the microorganism in the air on line has the advantages of reducing the difficulty of detection operation, increasing the detection precision by on-line detection and effectively improving the detection efficiency, solves the problems of low detection efficiency, little effect and very complicated detection process of the concentration of the adenosine triphosphate at present, can reduce the consumption of instruments and human resources, and has important significance for monitoring and forecasting the aerosol of the microorganism such as virus, bacteria and the like in the air.
Description
Technical Field
The invention relates to the technical field of air environment detection, in particular to an on-line detection method for the concentration of adenosine triphosphate of microorganisms in air.
Background
The microorganisms in the air mainly come from soil, water surface, animals and plants, human body and living activities, sewage treatment substances and the like, the composition concentration of the microorganisms is unstable, the microorganisms are various and comprise bacteria, fungi, viruses, phages and the like, the microorganisms in the air exist in the form of aerosol, and the aerosol is a dispersion system in which solid or liquid particles are suspended in a gas medium. Airborne dust, particulate matter or liquid droplets with microorganisms are microbial aerosols. The amount of airborne microorganisms is one of the important criteria for quality control. Under the conditions of high humidity, much dust, poor ventilation and insufficient sunlight, the microorganisms in the air are more in quantity and have longer survival time. Microorganisms pollute the air, which can make the air a vehicle for transmitting respiratory infectious diseases.
Adenosine Triphosphate (ATP) is a small molecular high-energy phosphate compound and also is a main energy source in organic life bodies, and energy supply of various vital activities of cells is guaranteed. As an "energy currency", it participates in many important physiological processes in living beings, such as: gene synthesis, nutrient metabolism, drug delivery, and regulation of immune and neural-mediated biological activities. Related researches show that the change of ATP content as an indicator of cell viability and cell damage of organic life bodies is closely related to the occurrence of diseases such as cardiovascular diseases, Parkinson's disease, Alzheimer's disease and the like. In addition, in the field of food safety, quantitative detection of ATP is also used for detection of food-borne pathogenic microorganisms. Therefore, the ATP quantitative detection method with high sensitivity and high specificity is established, and has a vital role in the fields of life science research, clinical diagnosis, food safety, environmental analysis and the like.
When human beings face similar known and unknown viruses, the detection of bioaerosols such as viruses, bacteria and microorganisms in the air is very necessary. The existing detection method for the concentration of adenosine triphosphate of microorganisms in the air has low detection efficiency, little effect, very complicated process and huge instrument and manpower consumption.
Disclosure of Invention
The invention aims to provide an on-line detection method for the concentration of adenosine triphosphate of microorganisms in air, which solves the problems of low detection efficiency, little effect and very complicated detection process of the existing detection method for the concentration of adenosine triphosphate of microorganisms in air.
In order to achieve the purpose, the invention provides the following technical scheme: an on-line detection method for the concentration of adenosine triphosphate of microorganisms in the air comprises the following steps:
s1: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device;
s2: the rotational flow sampler is injected with a test agent, and microorganisms in the air are absorbed by the test agent in the rotational flow process;
s3: the back of the rotational flow sampler is connected with a bubble remover, and waste gas at the top end of the bubble remover is pumped out by an air pump;
s4: the test agent at the lower end of the bubble remover does not contain bubbles, and the peristaltic pump works to convey the test agent to the stabilizing pipe and the detector;
s5: a diode is arranged in the detector and used as exciting light to irradiate the test agent in the detector pipeline, and finally, the fluorescence spectrum is recorded through a spectrometer.
Preferably, in the step S1, the rate of introducing the air collected during the detection process into the condensation chamber is 500 to 1500 ml/min.
Preferably, in the step S1, the rotational flow sampling device is made of glass, the inner diameter of the rotational flow sampling device is 1-5 mm, and the gas flowing distance is 10-20 cm.
Preferably, in step S2, the peristaltic pump drives the test agent to inject into the rotational flow sampler, and the flow rate of the test agent is controlled to be 0.1-10 ml/min.
Preferably, in the step S3, the bubble remover stands after the work is completed, and the standing time is 5-10 min.
Preferably, in step S4, the stabilizer tube is made of teflon, and has an inner diameter of 1-2 mm and a length of 1-3 m.
Preferably, in the step S4, the detector is made of polytetrafluoroethylene material, the liquid passes through the right-angle sections of the pipelines of the 3 detectors, and the inner diameter of the pipeline in the detector is 1-2 mm.
Preferably, in step S5, a diode of 400-450 nm is placed in the first right-angle segment of the detector as the excitation light.
Preferably, in step S5, a 500-550 nm spectrometer is placed in the second right-angle section of the detector, and the fluorescence spectrum is recorded.
Preferably, in step S5, the diode and the detector, and the spectrometer and the detector are connected by an optical fiber.
Compared with the prior art, the invention has the following beneficial effects:
the method for detecting the concentration of the adenosine triphosphate of the microorganism in the air on line has the advantages of reducing the difficulty of detection operation, increasing the detection precision by on-line detection and effectively improving the detection efficiency, solves the problems of low detection efficiency, little effect and very complicated detection process of the concentration of the adenosine triphosphate at present, can reduce the consumption of instruments and human resources, and has important significance for monitoring and forecasting the aerosol of the microorganism such as virus, bacteria and the like in the air.
Drawings
FIG. 1 is a schematic flow chart of the detection method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
an on-line detection method for the concentration of adenosine triphosphate of microorganisms in the air comprises the following steps:
s1: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device, the speed of introducing the collected air into the condensing chamber in the detection process is 800ml/min, the rotational flow sampling device is made of glass materials, the inner diameter of the rotational flow sampling device is 1mm, and the gas flowing distance is 10 cm;
s2: the test agent is injected into the rotational flow sampler, microorganisms in the air are absorbed by the test agent in the rotational flow process, the test agent is driven by the peristaltic pump to be injected into the rotational flow sampler, and the flow rate of the test agent is controlled to be 2 ml/min;
s3: the back of the rotational flow sampler is connected with a bubble remover, waste gas at the top end of the bubble remover is pumped out by an air pump, the bubble remover stands for 5min after working is finished, the separation of bubbles from a test agent is ensured, and the test agent is prevented from containing bubbles;
s4: the test agent at the lower end of the bubble remover does not contain bubbles, the peristaltic pump works to convey the test agent to a stabilizing pipe and a detector, the stabilizing pipe is made of polytetrafluoroethylene material, the inner diameter of the stabilizing pipe is 1mm, the length of the stabilizing pipe is 1m, the detector is made of polytetrafluoroethylene material, liquid passes through the right-angle sections of 3 detector pipelines, and the inner diameter of the pipeline in the detector is 1 mm;
s5: the diode is arranged in the detector and used as exciting light to irradiate a test agent in a pipeline of the detector, the fluorescence spectrum is recorded through the spectrometer, the diode with the wavelength of 400-450 nm is placed in the first right-angle section of the detector and used as the exciting light, the spectrometer with the wavelength of 500-550 nm is placed in the second right-angle section of the detector and used for recording the fluorescence spectrum, and the diode and the detector and the spectrometer and the detector are connected through optical fibers, so that the data transmission rate is increased, and the signal delay is avoided.
Example two:
an on-line detection method for the concentration of adenosine triphosphate of microorganisms in the air comprises the following steps:
s1: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device, the speed of introducing the collected air into the condensing chamber in the detection process is 1000ml/min, the rotational flow sampling device is made of glass materials, the inner diameter of the rotational flow sampling device is 3mm, and the gas flowing distance is 15 cm;
s2: the test agent is injected into the rotational flow sampler, microorganisms in the air are absorbed by the test agent in the rotational flow process, the test agent is driven by the peristaltic pump to be injected into the rotational flow sampler, and the flow rate of the test agent is controlled to be 6 ml/min;
s3: the back of the rotational flow sampler is connected with a bubble remover, waste gas at the top end of the bubble remover is pumped out by an air pump, the bubble remover stands after working is finished, the standing time is 7.5min, the separation of bubbles from a test agent is ensured, and the test agent is prevented from containing bubbles;
s4: the test agent at the lower end of the bubble remover does not contain bubbles, the peristaltic pump works to convey the test agent to a stabilizing pipe and a detector, the stabilizing pipe is made of polytetrafluoroethylene materials, the inner diameter of the stabilizing pipe is 1.5mm, the length of the stabilizing pipe is 2m, the detector is made of polytetrafluoroethylene materials, liquid passes through the right-angle sections of 3 detector pipelines, and the inner diameter of the pipelines in the detector is 1.5 mm;
s5: the diode is arranged in the detector and used as exciting light to irradiate a test agent in a pipeline of the detector, the fluorescence spectrum is recorded through the spectrometer, the diode with the wavelength of 400-450 nm is placed in the first right-angle section of the detector and used as the exciting light, the spectrometer with the wavelength of 500-550 nm is placed in the second right-angle section of the detector and used for recording the fluorescence spectrum, and the diode and the detector and the spectrometer and the detector are connected through optical fibers, so that the data transmission rate is increased, and the signal delay is avoided.
Example three:
an on-line detection method for the concentration of adenosine triphosphate of microorganisms in the air comprises the following steps:
s1: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device, the rate of introducing the collected air into the condensing chamber in the detection process is 1500ml/min, the rotational flow sampling device is made of glass materials, the inner diameter of the rotational flow sampling device is 5mm, and the gas flowing distance is 20 cm;
s2: the test agent is injected into the rotational flow sampler, microorganisms in the air are absorbed by the test agent in the rotational flow process, the test agent is driven by the peristaltic pump to be injected into the rotational flow sampler, and the flow rate of the test agent is controlled to be 10 ml/min;
s3: the back of the rotational flow sampler is connected with a bubble remover, waste gas at the top end of the bubble remover is pumped out by an air pump, the bubble remover stands for 10min after working is finished, the separation of bubbles from a test agent is ensured, and the test agent is prevented from containing bubbles;
s4: the test agent at the lower end of the bubble remover does not contain bubbles, the peristaltic pump works to convey the test agent to a stabilizing pipe and a detector, the stabilizing pipe is made of polytetrafluoroethylene material, the inner diameter of the stabilizing pipe is 2mm, the length of the stabilizing pipe is 3m, the detector is made of polytetrafluoroethylene material, liquid passes through the right-angle sections of 3 detector pipelines, and the inner diameter of the pipeline in the detector is 2 mm;
s5: the diode is arranged in the detector and used as exciting light to irradiate a test agent in a pipeline of the detector, the fluorescence spectrum is recorded through the spectrometer, the diode with the wavelength of 400-450 nm is placed in the first right-angle section of the detector and used as the exciting light, the spectrometer with the wavelength of 500-550 nm is placed in the second right-angle section of the detector and used for recording the fluorescence spectrum, and the diode and the detector and the spectrometer and the detector are connected through optical fibers, so that the data transmission rate is increased, and the signal delay is avoided.
The method for detecting the concentration of the adenosine triphosphate of the microorganism in the air on line has the advantages of reducing the difficulty of detection operation, increasing the detection precision by on-line detection and effectively improving the detection efficiency, solves the problems of low detection efficiency, little effect and very complicated detection process of the concentration of the adenosine triphosphate at present, can reduce the consumption of instruments and human resources, and has important significance for monitoring and forecasting the aerosol of the microorganism such as virus, bacteria and the like in the air.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. An on-line detection method for the concentration of adenosine triphosphate of microorganisms in the air is characterized by comprising the following steps:
s1: firstly, collecting air and introducing the air into a condensing chamber, wherein airflow flows out of the condensing chamber and then enters a rotational flow sampling device;
s2: the rotational flow sampler is injected with a test agent, and microorganisms in the air are absorbed by the test agent in the rotational flow process;
s3: the back of the rotational flow sampler is connected with a bubble remover, and waste gas at the top end of the bubble remover is pumped out by an air pump;
s4: the test agent at the lower end of the bubble remover does not contain bubbles, and the peristaltic pump works to convey the test agent to the stabilizing pipe and the detector;
s5: a diode is arranged in the detector and used as exciting light to irradiate the test agent in the detector pipeline, and finally, the fluorescence spectrum is recorded through a spectrometer.
2. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S1, the speed of introducing the air collected in the detection process into the condensation chamber is 500-1500 ml/min.
3. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S1, the rotational flow sampling device is made of glass materials, the inner diameter of the rotational flow sampling device is 1-5 mm, and the gas flowing distance is 10-20 cm.
4. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S2, the peristaltic pump drives the test agent to be injected into the rotational flow sampler, and the flow rate of the test agent is controlled to be 0.1-10 ml/min.
5. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: and in the step S3, standing the bubble remover after the work is finished, wherein the standing time is 5-10 min.
6. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S4, the stabilizing pipe is made of polytetrafluoroethylene, the inner diameter of the stabilizing pipe is 1-2 mm, and the length of the stabilizing pipe is 1-3 m.
7. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in step S4, the detector is made by polytetrafluoroethylene material, and liquid passes through 3 detector pipeline right angle sections, and the pipeline internal diameter is 1 ~ 2mm in the detector.
8. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S5, a diode with the wavelength of 400-450 nm is placed at the first right-angle section of the detector to be used as exciting light.
9. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in the step S5, a 500-550 nm spectrometer is placed at the second right-angle section of the detector, and the fluorescence spectrum is recorded.
10. The method for on-line detection of the concentration of adenosine triphosphate of microorganisms in the air according to claim 1, wherein: in step S5, the diode and the detector, and the spectrometer and the detector are connected by optical fibers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110945356.2A CN113670871A (en) | 2021-08-17 | 2021-08-17 | Online detection method for concentration of adenosine triphosphate of microorganisms in air |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110945356.2A CN113670871A (en) | 2021-08-17 | 2021-08-17 | Online detection method for concentration of adenosine triphosphate of microorganisms in air |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113670871A true CN113670871A (en) | 2021-11-19 |
Family
ID=78543353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110945356.2A Pending CN113670871A (en) | 2021-08-17 | 2021-08-17 | Online detection method for concentration of adenosine triphosphate of microorganisms in air |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113670871A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181780A (en) * | 2022-09-14 | 2022-10-14 | 深圳市安保医疗感控科技股份有限公司 | Microorganism collection method, device, system and computer readable storage medium |
-
2021
- 2021-08-17 CN CN202110945356.2A patent/CN113670871A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181780A (en) * | 2022-09-14 | 2022-10-14 | 深圳市安保医疗感控科技股份有限公司 | Microorganism collection method, device, system and computer readable storage medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2195956C (en) | Cellular material detection apparatus and method | |
| EP0073773B1 (en) | Perfusion-cultivation of animal cells and equipment therefor | |
| Berthelot et al. | Assessment of the dinitrogen released as ammonium and dissolved organic nitrogen by unicellular and filamentous marine diazotrophic cyanobacteria grown in culture | |
| Blatny et al. | Airborne Legionella bacteria from pulp waste treatment plant: aerosol particles characterized as aggregates and their potential hazard | |
| Cho et al. | Continuous surveillance of bioaerosols on-site using an automated bioaerosol-monitoring system | |
| CN113670871A (en) | Online detection method for concentration of adenosine triphosphate of microorganisms in air | |
| CN102323115A (en) | Air sampler | |
| Rule et al. | Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans | |
| Seshadri et al. | Application of ATP bioluminescence method to characterize performance of bioaerosol sampling devices | |
| Heo et al. | Enriched aerosol-to-hydrosol transfer for rapid and continuous monitoring of bioaerosols | |
| CN104535479A (en) | Sub-terahertz nano biosensor for detecting single or few cells | |
| CN104502303A (en) | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof | |
| Stewart et al. | The enumeration of aerosolised Saccharomyces cerevisiae using bioluminescent assay of total adenylates | |
| Zhang et al. | Adenosine triphosphate (ATP) bioluminescence-based strategies for monitoring atmospheric bioaerosols | |
| CN106290272A (en) | A kind of method of real-time detection Atmospheric particulates bio-toxicity | |
| Ariya et al. | Bio-organic materials in the atmosphere and snow: measurement and characterization | |
| Tolchinsky et al. | Development of a personal bioaerosol sampler based on a conical cyclone with recirculating liquid film | |
| Clauß et al. | Development of a Novel Bioaerosol Chamber to Determine Survival Rates of Airborne Staphylococci | |
| Clauß et al. | Effective Collection of Airborne Micro-Organisms by Direct Impaction on Silicone Sealants—Comparison of Different Adherent Surfaces | |
| CN116601476A (en) | Air monitoring system and method | |
| CN102191162B (en) | Real time detecting apparatus and method for microbial activity in air | |
| Wang et al. | Design of UVA ultraviolet disinfection system for nutrient solution residual liquid and development of microbial online monitoring system | |
| CN215678082U (en) | Device for on-line detection of adenosine triphosphate concentration of microorganisms in air | |
| Liu et al. | Air sampling and ATP bioluminescence for quantitative detection of airborne microbes | |
| CN218157115U (en) | Online collection and detection device for bioaerosol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20211119 |