CN113661979B - Frozen tube rack capable of planting ice by ultrasonic and program cooling device - Google Patents
Frozen tube rack capable of planting ice by ultrasonic and program cooling device Download PDFInfo
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- CN113661979B CN113661979B CN202111098165.3A CN202111098165A CN113661979B CN 113661979 B CN113661979 B CN 113661979B CN 202111098165 A CN202111098165 A CN 202111098165A CN 113661979 B CN113661979 B CN 113661979B
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- ultrasonic vibrator
- rack body
- tube rack
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- 239000000463 material Substances 0.000 claims abstract description 4
- 230000008014 freezing Effects 0.000 claims description 29
- 238000007710 freezing Methods 0.000 claims description 25
- 230000005540 biological transmission Effects 0.000 claims description 23
- 230000000149 penetrating effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000005138 cryopreservation Methods 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000005496 eutectics Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000006911 nucleation Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000004781 supercooling Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
- A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Hematology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a frozen tube rack capable of being subjected to ultrasonic ice planting and a program cooling device, which belong to the field of cell frozen storage, wherein the frozen tube rack comprises a tube rack body, an ultrasonic vibrator and an ultrasonic generator, a loading groove is formed in the tube rack body, the ultrasonic generator is electrically connected with the ultrasonic vibrator, the ultrasonic vibrator is fixedly arranged on the tube rack body, and the tube rack body is made of rigid materials; the pipe rack body comprises a connecting rod, and bearing plates and fixing plates which are vertically fixed on the connecting rod and are sequentially arranged at intervals along the axial direction of the connecting rod, wherein at least two bearing plates are provided with loading holes, and the loading holes on each bearing plate are axially aligned to form loading grooves; the ultrasonic vibrator comprises a plurality of ultrasonic vibrators A arranged on the fixed plate and ultrasonic vibrators B fixedly connected with the bearing plate after penetrating through the fixed plate, and the ultrasonic vibrators A are arranged around the ultrasonic vibrators B. The invention has simple structure and reasonable design, and can be suitable for various program cooling devices.
Description
Technical Field
The invention relates to the field of cell cryopreservation, in particular to an ultrasonic ice planting cryopreservation pipe frame and a program cooling device.
Background
The low-temperature preservation of cells and tissues is a key technology for modern regenerative medicine, organ transplantation and assisted reproduction, and is widely paid attention to by all parties. The cell cryopreservation process is generally to freeze the cells to a lower subzero temperature, so as to realize long-term preservation, but the cells and tissues can be damaged in the cryopreservation process: firstly, toxic damage of a low-temperature protective agent; and secondly, physical damage caused by ice crystal formation in cells due to program cooling.
At present, cell freezing of a biological sample library is carried out by adopting a program cooling method, but in the traditional program cooling process, the solution temperature is still not frozen when being lower than the eutectic temperature, so that the cell solution is supercooled, and when the supercooling degree is larger, the cell solution is suddenly nucleated, so that the ice crystal is larger to cause physical damage. The method for planting ice includes such steps as adding ice core, touching the external surface of freezing tube with frozen metal, applying ultrasonic wave, and crystallizing the solution to form eutectic crystal. Researchers find that in the process of program cooling, a certain ice-planting operation is adopted to effectively improve the survival rate of cells, and as the ultrasonic wave can reduce the supercooling degree and improve the nucleation temperature by utilizing the cavitation effect of the ultrasonic wave, the ice-planting effect is obvious and the operation is simple, so that the ultrasonic wave ice-planting becomes the main research direction of an ice-planting method and has a positive effect on the preservation of biological samples.
However, the existing program cooling instrument does not have an ice-planting function, and ice cannot be planted automatically, and usually an operator needs to take out cells reduced to a specific temperature from the program cooling instrument and touch the outer wall of the freezing tube by using a precooling tool such as forceps to finish ice-planting operation. However, the scheme is not widely adopted due to complex operation, large workload and low efficiency, and the popularization and the use of the existing program cooling instrument and the large-scale development of a biological sample library are hindered. The Chinese patent application numbers CN2019108703169, CN2021100517268 and CN2021106803865 all disclose program freezing boxes with ultrasonic ice planting function, which have good effect on preserving cells, but each self-designed program freezing box is only suitable for partial experiments and has great limitation. Therefore, the automatic ice-planting function of the existing program cooling instrument is necessary, or the modification of the existing program cooling instrument is necessary, namely, the construction of the freezing storage rack with the ultrasonic ice-planting function is very important, so that the freezing storage rack can be adapted to the existing program freezing equipment without the ice-planting function.
Disclosure of Invention
Aiming at the problem that the program cooling device in the prior art has large use limitation and can not realize one machine for multiple purposes, the invention aims to provide a freezing pipe rack capable of ultrasonically planting ice and the program cooling device.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
the invention provides a freezing pipe rack capable of planting ice ultrasonically, which comprises a pipe rack body, wherein a loading groove for loading freezing pipes or freezing bags is formed in the pipe rack body; the ultrasonic wave vibrator is fixedly mounted on the pipe rack body, and the pipe rack body is made of rigid materials.
Preferably, the pipe rack body comprises a connecting rod, and a bearing plate and a fixing plate which are vertically fixed on the connecting rod and are sequentially arranged at intervals along the axis direction of the connecting rod; the bearing plates comprise a bearing plate body and a plurality of loading holes arranged on the bearing plate body, and the loading holes on the bearing plates are axially aligned to form the loading grooves.
Preferably, the ultrasonic vibrator comprises an ultrasonic vibrator A, and the ultrasonic vibrator A is fixedly arranged on one surface of the fixing plate, which is away from the bearing plate.
Preferably, the number of the connecting rods and the number of the ultrasonic vibrators are plural, and the plurality of the ultrasonic vibrators a are respectively fixed in the preset distances of the plurality of connecting rods.
Preferably, the bearing plate and the fixing plate are rectangular, and the four connecting rods are respectively connected to the four corners of the bearing plate and the fixing plate.
Further, the ultrasonic vibrator further comprises an ultrasonic vibrator B, a through hole is further formed in the fixing plate, and one end portion of the ultrasonic vibrator B penetrates through the through hole and is fixedly connected with one bearing plate adjacent to the fixing plate.
Preferably, a plurality of the ultrasonic transducers a are arranged on the same circumference centering on the ultrasonic transducer B.
Further, the wireless power transmission device is used for supplying power to the ultrasonic generator and comprises a wireless power transmission transmitting end and a wireless power transmission receiving end, the wireless power transmission receiving end is electrically connected with the ultrasonic generator through a cable, and the wireless power transmission transmitting end is connected with a plug through the cable.
Preferably, the ultrasonic generator is connected with a plug through a cable.
In a second aspect, the invention also provides a program cooling device, which comprises a main body and the freezing pipe frame capable of planting ice by ultrasonic waves.
By adopting the technical scheme, the ultrasonic vibrators are directly arranged on the freezing pipe rack, so that the program cooling devices of various types and models do not need to be specially modified, the program cooling devices can have the ultrasonic ice planting function by replacing the freezing pipe rack, freezing operation of cells can be performed at low cost and high efficiency, and the application range of the program cooling devices is widened.
Drawings
FIG. 1 is a schematic view of a structure of the present invention in one direction;
FIG. 2 is a schematic view of another direction of the present invention;
FIG. 3 is a front view of the present invention;
FIG. 4 is a top view of the present invention;
fig. 5 is a bottom view of the present invention.
In the figure, the ultrasonic wave power transmission device comprises a 1-pipe rack body, 11-connecting rods, 12-bearing plates, 13-fixing plates, 2-loading grooves, 3-ultrasonic vibrators, 31-ultrasonic vibrators A, 32-ultrasonic vibrators B, 4-ultrasonic generators, 5-plugs, 6-wireless power transmission devices, 61-wireless power transmission transmitting ends and 62-wireless power transmission receiving ends.
Detailed Description
The following describes the embodiments of the present invention further with reference to the drawings. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
It should be noted that, in the description of the present invention, the positional or positional relation indicated by the terms such as "upper", "lower", "left", "right", "front", "rear", etc. are merely for convenience of describing the present invention based on the description of the structure of the present invention shown in the drawings, and are not intended to indicate or imply that the apparatus or element to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
The terms "first" and "second" in this technical solution are merely references to the same or similar structures, or corresponding structures that perform similar functions, and are not an arrangement of the importance of these structures, nor are they ordered, or are they of a comparative size, or other meaning.
In addition, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., the connection may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two structures. It will be apparent to those skilled in the art that the specific meaning of the terms described above in this application may be understood in the light of the general inventive concept in connection with the present application.
Example 1
The frozen tube rack capable of being subjected to ultrasonic ice planting comprises a tube rack body 1, wherein a loading groove 2 for loading frozen tubes or frozen bags is formed in the tube rack body 1, as shown in fig. 1-5; the ultrasonic wave vibrator 3 and the ultrasonic generator 4 are also included simultaneously, the ultrasonic generator 4 is electrically connected with the ultrasonic vibrator 3 through a cable, the ultrasonic generator 4 is connected with the plug 5 through the cable, the ultrasonic vibrator 3 is fixedly arranged on the pipe rack body 1, and the pipe rack body 1 is made of rigid materials.
In this embodiment, the pipe rack body 1 specifically includes a connecting rod 11, and a carrying plate 12 and a fixing plate 13 vertically fixed on the connecting rod 11 and sequentially arranged at intervals along the axial direction of the connecting rod 11.
The carrier plate 12 includes a carrier plate body and a plurality of loading holes disposed on the carrier plate body, and at least two carrier plates 12 are mounted in this example, for example, two carrier plates, and the loading holes on the two carrier plates 12 are axially aligned, so that each two aligned loading holes form one loading slot 2. The fixing plate 13 is mainly attached to the ultrasonic transducer 3. The connecting rods 11 are used for fixedly connecting the bearing plates 12 and the fixing plates 13 into a whole, and in order to maintain the stability of the structure, a plurality of connecting rods 11 are arranged and distributed, for example, in this embodiment, the bearing plates 12 and the fixing plates 13 are specifically configured to have rectangular structures, four connecting rods 11 are correspondingly configured and respectively arranged at four corners of the bearing plates 12 and the fixing plates 13, in a specific connection mode, preferably, the upper ends of the connecting rods 11 are welded on the bottom surface of one of the bearing plates 12, the lower ends of the connecting rods 11 are welded on the top surface of the fixing plates 13, and the other bearing plates 12 are correspondingly provided with mounting holes through which the connecting rods 11 pass, and the connecting rods 11 and the other bearing plates 12 are welded and fixed at the positions of the mounting holes, so that the pipe rack body 1 can be manufactured.
The shape of the ultrasonic vibrator 3 may be either a sheet shape or a ring shape, and in this embodiment, it is preferable that the ultrasonic vibrator 3 includes a plurality of ultrasonic vibrators a31, and the plurality of ultrasonic vibrators a31 are fixedly mounted on a surface of the fixing plate 13 facing away from the carrier plate 12, that is, a bottom surface of the fixing plate 13. When the ice planting device is used, the ultrasonic vibrator A31 transmits vibration to the connecting rod 11 through the fixing plate 13 and transmits the vibration to the two bearing plates 12 through the connecting rod 11, so that the freezing storage tube or the freezing storage bag in the loading groove 2 is forced to vibrate, and further the ice planting effect is generated. And it is understood that in order to make the vibration of the ultrasonic vibrator a31 be better transmitted to the carrier plate 12, further, the mounting position of the ultrasonic vibrator a31 on the fixing plate 13 is located near the connecting rod 11, for example, the ultrasonic vibrator a31 is configured with four, four ultrasonic vibrators a31 are connected in series, and the four ultrasonic vibrators a31 are fixed within preset distances of the four connecting rods 11, respectively, which can be flexibly controlled as needed.
When the program freezing device, such as a program freezer, needs to perform ice planting operation in the process of cell freezing, the pipe rack body 1 can be installed in the program freezer, the ultrasonic generator 4 is positioned at a position outside the cooling area, and the ultrasonic generator 4 is connected with electricity through the plug 5; the freezing tube or the freezing bag loaded with the cells to be frozen is placed in the loading groove 2, freezing operation can be performed, when the temperature is reduced to the ice planting temperature, the ultrasonic vibrator 3 is driven to work through the ultrasonic generator 4, and the ice planting phenomenon is caused to the solution through the nucleation effect of the ultrasonic wave by applying the ultrasonic wave to the freezing tube or the freezing bag, so that ice crystals are prevented from being generated inside the cells, and the cells are protected.
Example two
It differs from embodiment one in that: in this embodiment, the ultrasonic transducer 3 further includes an ultrasonic transducer B32, the ultrasonic transducer B32 is connected in series with the ultrasonic transducer a31, and the fixing plate 13 is further provided with a through hole, and one end (upper end) of the ultrasonic transducer B32 passes through the through hole and is fixedly connected to the bottom surface of one of the carrier plates 12 adjacent to the fixing plate 13. This arrangement makes it possible to obtain ultrasonic waves as much as possible everywhere on the carrier plate 12.
Further, in the arrangement form, a plurality of ultrasonic vibrators a31 are arranged on the same circumference centering on the ultrasonic vibrator B32. The arrangement is such that at the edge, ultrasonic waves can be transmitted to the carrier plate 12 by the ultrasonic vibrator a31, and at the middle, ultrasonic waves can be transmitted to the carrier plate 12 by the ultrasonic vibrator B32.
Example III
It differs from embodiment one in that: the ultrasonic generator 4 in this embodiment obtains the supply of electric energy by another way, that is, the scheme of this embodiment includes a wireless power transmission device 6 for supplying power to the ultrasonic generator 4, the wireless power transmission device 6 includes a wireless power transmission transmitting end 61 and a wireless power transmission receiving end 62, wherein the wireless power transmission receiving end 62 is electrically connected with the ultrasonic generator 4 through a cable, and the wireless power transmission transmitting end 61 is connected with the plug 5 through the cable.
So set up, make when the program cooling appearance is inconvenient to directly provide the socket to plug 5, and do not have the through wires hole that supplies the cable to wear out, can arrange wireless transmission receiving terminal 62 and wireless transmission transmitting terminal 61 respectively in the inside and outside both sides of program cooling appearance hatch door to realize contactless transmission.
Example IV
A program cooling device comprises a main body and a freezing pipe rack capable of planting ice by ultrasonic waves as disclosed in any embodiment.
The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, and yet fall within the scope of the invention.
Claims (4)
1. The utility model provides a frozen tube frame that can supersound plant ice which characterized in that: the freezing storage rack comprises a rack body, wherein a loading groove for loading freezing storage pipes or freezing storage bags is formed in the rack body; the ultrasonic wave generating device comprises a pipe rack body, and is characterized by further comprising an ultrasonic vibrator and an ultrasonic generator, wherein the ultrasonic generator is electrically connected with the ultrasonic vibrator, the ultrasonic vibrator is fixedly arranged on the pipe rack body, and the pipe rack body is made of a rigid material;
the pipe rack body comprises a connecting rod, and a bearing plate and a fixing plate which are vertically fixed on the connecting rod and are sequentially arranged at intervals along the axis direction of the connecting rod; the bearing plates comprise a bearing plate body and a plurality of loading holes arranged on the bearing plate body, and the loading holes on at least two bearing plates are axially aligned to form the loading groove;
the ultrasonic vibrator comprises an ultrasonic vibrator A and an ultrasonic vibrator B, the ultrasonic vibrator A is connected with the ultrasonic vibrator B in series, the ultrasonic vibrator A is fixedly arranged on one surface of the fixing plate, which is away from the bearing plate, a through hole is further formed in the fixing plate, and one end part of the ultrasonic vibrator B penetrates through the through hole and is fixedly connected with one bearing plate adjacent to the fixing plate;
the ultrasonic vibrator B is arranged on the same circumference taking the ultrasonic vibrator B as the center, and the ultrasonic vibrator A is fixed on the same circumference taking the ultrasonic vibrator B as the center;
the wireless power transmission device comprises a wireless power transmission transmitting end and a wireless power transmission receiving end, wherein the wireless power transmission receiving end is electrically connected with the ultrasonic generator through a cable, and the wireless power transmission transmitting end is connected with a plug through the cable.
2. The ultrasonically implantable frozen tube rack of claim 1, wherein: the bearing plate and the fixing plate are rectangular, and the number of the connecting rods is four, and the four connecting rods are respectively connected with the four corners of the bearing plate and the fixing plate.
3. The ultrasonically implantable frozen tube rack of claim 1, wherein: the ultrasonic generator is connected with a plug through a cable.
4. A program cooling device, characterized in that: a frozen tube rack comprising a main body and an ultrasonically implantable ice according to any one of claims 1 to 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111098165.3A CN113661979B (en) | 2021-09-18 | 2021-09-18 | Frozen tube rack capable of planting ice by ultrasonic and program cooling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111098165.3A CN113661979B (en) | 2021-09-18 | 2021-09-18 | Frozen tube rack capable of planting ice by ultrasonic and program cooling device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113661979A CN113661979A (en) | 2021-11-19 |
| CN113661979B true CN113661979B (en) | 2024-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111098165.3A Active CN113661979B (en) | 2021-09-18 | 2021-09-18 | Frozen tube rack capable of planting ice by ultrasonic and program cooling device |
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| Country | Link |
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| CN (1) | CN113661979B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114903029A (en) * | 2022-05-16 | 2022-08-16 | 苏州碳卡智能科技有限公司 | Automatic program cooling control method |
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| CN205413120U (en) * | 2016-03-30 | 2016-08-03 | 李位芳 | Portable test -tube rack |
| CN110487009A (en) * | 2018-05-15 | 2019-11-22 | 青岛海尔智能技术研发有限公司 | A kind of refrigerating equipment |
| CN113207872A (en) * | 2021-06-18 | 2021-08-06 | 上海理工大学 | Cell planting ice freezing method and device |
| CN216147114U (en) * | 2021-09-18 | 2022-04-01 | 上海理工大学 | But freezing pipe support and procedure heat sink of ice are planted to supersound |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112665310A (en) * | 2021-01-14 | 2021-04-16 | 上海海洋大学 | Ultrasonic rapid freezing equipment with vortex tube and freezing method |
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2021
- 2021-09-18 CN CN202111098165.3A patent/CN113661979B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105204689A (en) * | 2015-10-13 | 2015-12-30 | 杭州纳帕科技有限公司 | Kit for embedding touch screen on refrigerator door and application method of kit |
| CN205413120U (en) * | 2016-03-30 | 2016-08-03 | 李位芳 | Portable test -tube rack |
| CN110487009A (en) * | 2018-05-15 | 2019-11-22 | 青岛海尔智能技术研发有限公司 | A kind of refrigerating equipment |
| CN113207872A (en) * | 2021-06-18 | 2021-08-06 | 上海理工大学 | Cell planting ice freezing method and device |
| CN216147114U (en) * | 2021-09-18 | 2022-04-01 | 上海理工大学 | But freezing pipe support and procedure heat sink of ice are planted to supersound |
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| Publication number | Publication date |
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| CN113661979A (en) | 2021-11-19 |
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