CN113655125B - Image enhancement equipment based on animal tissue section development - Google Patents

Image enhancement equipment based on animal tissue section development Download PDF

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CN113655125B
CN113655125B CN202111041774.5A CN202111041774A CN113655125B CN 113655125 B CN113655125 B CN 113655125B CN 202111041774 A CN202111041774 A CN 202111041774A CN 113655125 B CN113655125 B CN 113655125B
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CN113655125A (en
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姚宏波
梁明辉
文丽波
宫学武
张萌
王月静
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Qiqihar Medical University
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    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/04—Analysing solids
    • G01N29/06—Visualisation of the interior, e.g. acoustic microscopy
    • G01N29/0654—Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00—Measuring arrangements characterised by the use of optical techniques
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00—Measuring arrangements characterised by the use of optical techniques
    • G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
    • G01B11/06—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B17/00—Measuring arrangements characterised by the use of infrasonic, sonic or ultrasonic vibrations
    • G01B17/08—Measuring arrangements characterised by the use of infrasonic, sonic or ultrasonic vibrations for measuring roughness or irregularity of surfaces
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    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
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    • G01N29/223—Supports, positioning or alignment in fixed situation
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    • G01N2291/00—Indexing codes associated with group G01N29/00
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Abstract

The invention relates to an image enhancement device based on animal tissue slice imaging, and relates to the technical field of animal tissue imaging. The device comprises a bearing mechanism, a fixing mechanism and a control mechanism, wherein the bearing mechanism comprises a bottom plate and a light sensitive plate connected with the bottom plate and is used for bearing the animal tissue slices; the fixing mechanism is connected with the bearing mechanism and comprises a sliding seat connected with one side of a bottom plate of the bearing mechanism, a supporting plate connected with the sliding seat, a rotating shaft connected with the supporting plate, a first motor connected with one end of the rotating shaft and a pressing plate connected with the other end of the rotating shaft, and the fixing mechanism is used for fixing the animal tissue slice; the scanning mechanism is connected with the fixing mechanism and comprises an ultrasonic line scanner for detecting the position and the flatness of the animal tissue slice and a camera for detecting the thickness of the animal tissue slice; when in use, the device can be better adapted to animal tissue slices with different sizes, and the detection stability and comprehensiveness of the animal tissue slices are improved, so that the accuracy of detection results is further improved.

Description

Image enhancement equipment based on animal tissue section development
Technical Field
The invention relates to the technical field of animal tissue imaging, in particular to an image enhancement device based on animal tissue slice imaging.
Background
The animal tissue is the basic structure of the multicellular animal composed of cells with similar morphological functions and intercellular substances, is divided into four kinds of epithelial tissue, connective tissue, muscle tissue and nerve tissue, which are mutually connected and interdependent in different proportions to form various organs and systems of the animal to complete various physiological activities, and is differentiated from original inner, middle and outer embryonic layers in the embryonic stage; animal tissues can be classified into four major groups, epithelial tissues, connective tissues, muscle tissues and nerve tissues, according to their common characteristics in terms of origin, morphological structure and function.
Animal tissue need show through the section when detecting, need use the section scanner when observing the section, but the section scanner can not be fine show sliced image is better, and the display effect can let sliced observation comprehensiveness have more subtle performance better.
The fixed effect of current image enhancement equipment based on animal tissue section development in the use is unstable on the one hand, slight displacement phenomenon appears easily, let the equipment can not be better carry out image enhancement auxiliary work, there is not better promotion to sliced display effect, the display effect does not have better promotion and lets the monitoring comprehensiveness and the imperceptibility of animal tissue section tissue not have better promotion, on the other hand, current image enhancement equipment based on animal tissue section development has the difference because of section unevenness and scanning process to the result that scanning mechanism control inaccuracy leads to the detection.
Disclosure of Invention
Therefore, the invention provides image enhancement equipment based on animal tissue slice imaging, which is used for overcoming the problem that the control of a scanning mechanism is not accurate in the scanning process of the image enhancement equipment based on animal tissue slice imaging in the prior art, so that the detection result has difference.
To achieve the above object, the present invention provides an image enhancement apparatus based on imaging of animal tissue slices, comprising:
the bearing mechanism is used for bearing the animal tissue slices and comprises a bottom plate and a light-sensitive plate connected with the bottom plate;
the fixing mechanism is connected with the bearing mechanism and used for fixing the animal tissue slices, and comprises a sliding seat connected with one side of a bottom plate of the bearing mechanism, a supporting plate connected with the sliding seat, a rotating shaft connected with the supporting plate, a first motor connected with one end of the rotating shaft and a pressing plate connected with the other end of the rotating shaft;
the scanning mechanism is connected with the fixing mechanism and comprises a light source used for emitting light beams to the light sensing plate, a first camera used for detecting the position of the animal tissue slice, an ultrasonic scanner used for scanning the animal tissue slice to obtain an animal tissue imaging image and slice flatness and a second camera used for detecting the thickness of the animal tissue slice;
a second motor used for driving the fixing mechanism to move along the horizontal direction of the bottom plate and a third motor used for driving the fixing mechanism to move along the vertical direction of the bottom plate are arranged in the bottom plate, and a light transmission instrument used for detecting the light transmittance of the animal tissue slices is also arranged in the position, close to the light sensing plate, in the bottom plate;
bear mechanism, fixed establishment and scanning mechanism and be connected with the control unit respectively, it is right when animal tissue section scans, the control unit acquires animal tissue section's the position of placing to when confirming the position of placing and accomplishing, acquire animal tissue section's section thickness and section roughness, and according to section thickness and section roughness real-time right the turned angle of first motor is adjusted in order to fix animal tissue section, the luminance of light source and the height of clamp plate are adjusted in order to strengthen the video picture.
Further, when the animal tissue slice is scanned to obtain the imaging image, the control unit obtains the flatness U and the thickness H of the animal tissue slice according to the scanning image scanned by the ultrasonic scanner and the shooting image shot by the second camera, the control unit compares the flatness U with a preset flatness Uy and compares the thickness H with a preset thickness range H0, the control unit determines the ultrasonic wave length for scanning the animal tissue slice according to the comparison results of the flatness U with a preset flatness U0 and the thickness H with a preset thickness range H0, wherein the preset thickness range H0 comprises a preset thickness range minimum value Hmin and a preset thickness range maximum value Hmax, and Hmin is set to be less than Hmax,
the control unit is also provided with a first preset ultrasonic wavelength lambda 1 and a second preset ultrasonic wavelength lambda 2, wherein lambda 1 is less than lambda 2;
if U is larger than or equal to Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a first preset ultrasonic wavelength lambda 1;
if U is less than Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a second preset ultrasonic wavelength lambda 2;
if U is not less than Uy and
Figure GDA0003595358620000031
the control unit respectively compares the thickness H with a preset minimum thickness range Hmin and a preset maximum thickness range Hmax;
if U < Uy and
Figure GDA0003595358620000032
the control unit determines that the animal tissue section is not qualified.
Further, when the control unit compares the thickness H with a preset thickness range minimum Hmin and a preset thickness range maximum Hmax, if H is less than Hmin, the control unit preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is a first preset ultrasonic wavelength lambda 1, calculates a first thickness difference value delta Ha between the thickness H and the preset thickness range minimum Hmin, selects a corresponding adjusting coefficient according to the comparison result of the first thickness difference value and the preset thickness difference value to adjust the ultrasonic wavelength of the ultrasonic scanner,
wherein the control unit is also provided with a first preset thickness difference delta H1, a second preset thickness difference delta H2, a third preset thickness difference delta H3, a first ultrasonic wave length adjustment coefficient K1, a second ultrasonic wave length adjustment coefficient K2 and a third ultrasonic wave length adjustment coefficient K3, wherein delta H1 is more than delta H2 and less than delta H3,0.5 is more than K3 and more than K2 and more than K1 and less than 1,
when the delta H1 is more than or equal to the delta Ha and less than the delta H2, the control unit selects a first ultrasonic wave length adjusting coefficient K1 to adjust the first preset ultrasonic wave length lambda 1;
when the delta H2 is more than or equal to the delta Ha and less than the delta H3, the control unit selects a second ultrasonic wave length adjusting coefficient K2 to adjust the first preset ultrasonic wave length lambda 1;
when the delta Ha is larger than or equal to the delta H3, the control unit selects a third ultrasonic wave length adjusting coefficient K3 to adjust the first preset ultrasonic wave length lambda 1;
when the control unit selects the ith ultrasonic wave length adjusting coefficient Ki to adjust the first preset ultrasonic wave length, setting i to be 1, 2 and 3, and setting the adjusted first preset ultrasonic wave length lambda 1 to be lambda 1 'and setting lambda 1' to be lambda 1 multiplied by Ki by the control unit.
Further, if H is more than 1.5Hmax, the control unit judges that the animal tissue slice is unqualified, if Hmax is more than H and less than 1.5Hmax, the control unit preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is the second preset ultrasonic wavelength lambda 2, calculates the second thickness difference delta Hb between the thickness H and the maximum value Hmax of the preset thickness range, selects a corresponding adjusting coefficient according to the comparison result of the second thickness difference and the preset thickness difference to adjust the ultrasonic length of the ultrasonic scanner,
wherein the control unit is also provided with a fourth ultrasonic wave length adjusting coefficient K4, a fifth ultrasonic wave length adjusting coefficient K5 and a sixth ultrasonic wave length adjusting coefficient K6, wherein K4 is more than 1 and K4 is more than 1 and K6 is more than 2,
when the delta H1 is not less than the delta Hb which is less than the delta H2, the control unit selects a fourth ultrasonic wave length adjusting coefficient K4 to adjust the second preset ultrasonic wave length lambda 2;
when the delta H2 is not less than the delta Hb which is less than the delta H3, the control unit selects a fifth ultrasonic wave length adjusting coefficient K5 to adjust the second preset ultrasonic wave length lambda 2;
when the delta Hb is larger than or equal to the delta H3, the control unit selects a sixth ultrasonic wave length adjusting coefficient K6 to adjust the second preset ultrasonic wave length lambda 2;
when the control unit selects the j-th ultrasonic wave length adjustment coefficient Kj to adjust the first preset ultrasonic wave length, j is set to be 4, 5 and 6, the control unit sets the adjusted second preset ultrasonic wave length lambda 2 to be lambda 2 ', and sets lambda 2' to be lambda 2 multiplied by Kj.
Further, when the control unit controls the ultrasonic scanner to obtain the imaging image, the control unit obtains the preliminarily determined light source brightness G, if U is less than Uy and H belongs to H0, the control unit calculates the flatness difference value delta U between the flatness and the preset flatness U0, selects a corresponding brightness adjusting coefficient according to the comparison result of the flatness difference value and the preset flatness difference value to adjust the light source brightness,
wherein the control unit is further provided with a first preset flatness difference delta U1, a second preset flatness difference delta U2, a third preset flatness difference delta U3, a first brightness adjustment coefficient X1, a second brightness adjustment coefficient X2 and a third brightness adjustment coefficient X3, wherein delta U1 is more than delta U2 and less than delta U3,1 is more than X1 and more than X2 and more than X3 and less than 2,
when the delta U1 is not less than the delta U < delta U2, the control unit selects a first brightness adjusting coefficient X1 to adjust the brightness of the light source;
when the delta U2 is not less than the delta U < delta U3, the control unit selects a second brightness adjusting coefficient X2 to adjust the brightness of the light source;
when the delta U is larger than or equal to the delta U3, the control unit selects a third brightness adjusting coefficient X3 to adjust the brightness of the light source;
when the control unit selects the e-th brightness adjustment coefficient Xe to adjust the brightness of the light source, e is set to 1, 2, 3, and the control unit sets the adjusted brightness of the light source to G' which is set to G × Xe.
Further, when the control unit controls the light transmission instrument to move to the position corresponding to the animal tissue slice and obtains the numerical value of the light transmission instrument, the numerical value T of the light transmission instrument is compared with the preset light transmittance T0, if the T is more than or equal to T0, the control unit sets the light source brightness as the regulated light source brightness G', if the T is less than T0, the control unit calculates the light transmittance difference Delta T between the numerical value T of the light transmission instrument and the preset light transmittance T0, and selects the corresponding brightness correction coefficient according to the comparison result of the light transmittance difference and the preset light transmittance difference to correct the light source brightness,
wherein the control unit is further provided with a first preset light transmittance difference delta T1, a second preset light transmittance difference delta T2, a third preset light transmittance difference delta T3, a first brightness correction coefficient C1, a second brightness correction coefficient C2 and a third brightness correction coefficient C3, wherein delta T1 is more than delta T2 and is more than delta T3, 1 is more than C1 and is more than C2 and is more than C3 and is less than 1.5,
when the delta T1 is not less than the delta T < delta T2, the control unit selects a first brightness correction coefficient C1 to correct the brightness of the light source;
when the delta T2 is not less than the delta T < delta T3, the control unit selects a third brightness correction coefficient C2 to correct the brightness of the light source;
when the delta T is larger than or equal to the delta T3, the control unit selects a third brightness correction coefficient C3 to correct the brightness of the light source;
when the control unit selects the n-th luminance correction coefficient Cn to correct the luminance of the light source, setting n ═ 1, 2, 3, the control unit sets the luminance of the light source after correction to G ' ″, setting G ' = G ' × Cn.
Further, when the control unit controls to scan the animal tissue slice, the control unit preliminarily determines the brightness of the light source according to the comparison result of the thickness H of the animal tissue slice and the preset thickness,
wherein the control unit is also provided with a first preset thickness H1, a second preset thickness H2, a third preset thickness H3, a first light source brightness G1, a second light source brightness G2 and a third light source brightness G3, H1 is more than H2 is more than H3, G1 is more than G2 is more than G3,
when H1 is less than or equal to H < H2, the control unit sets the brightness of the light source to be the first light source brightness G1;
when H2 is less than or equal to H < H3, the control unit sets the brightness of the light source to be the second light source brightness G2;
when H is equal to or greater than H3, the control unit sets the brightness of the light source to be the third light source brightness G3.
Further, the control unit is further provided with a preset maximum light source luminance Gmax, when the control unit completes the light source luminance correction, the control unit compares the corrected light source luminance G ″ with the preset maximum light source luminance Gmax, if G ' > Gmax, the control unit calculates a luminance difference Δ G between the corrected light source luminance G ' ″ and the preset maximum light source luminance Gmax, sets Δ G ═ G ' -Gmax, and selects a corresponding correction coefficient to correct the second preset hypersonic wave length based on a result of the comparison of the luminance difference and the preset luminance difference,
wherein the control unit is further provided with a first preset brightness difference value delta G1, a second preset brightness difference value delta G2, a third preset brightness difference value delta G3, a first wavelength correction coefficient W1, a second wavelength correction coefficient W2 and a third wavelength correction coefficient W3, wherein delta G1 < delta G2 < delta G3, 0.5 < W3 < W2 < W1 < 1 are set,
when the delta G1 is not less than delta G and is less than delta G2, the control unit selects a first wavelength correction coefficient W1 to correct the preset ultrasonic wavelength;
when the delta G2 is not less than delta G and is less than delta G3, the control unit selects a second wavelength correction coefficient W2 to correct the preset ultrasonic wavelength;
when the delta G is larger than or equal to the delta G3, the control unit selects a third wavelength correction coefficient W3 to correct the preset ultrasonic wavelength;
when the control unit selects the s-th wavelength correction coefficient Ws to correct the preset ultrasonic wavelength, s is set to 1, 2, 3, and the control unit sets the corrected ultrasonic wavelength to λ 3, and sets λ 3 to λ 1 '× Ws or λ 3 to λ 2' × Ws.
Compared with the prior art, the animal tissue slice fixing device has the advantages that the animal tissue slice fixing stability can be better guaranteed in the use process of the device through the matching work among the sliding seat, the fixing rod, the seat body, the balls, the limiting plate, the supporting plate, the rotating shaft, the pressing plate and the rubber plate, the pressing plate can better drive the rubber plate to carry out the fixing work through the rotating shaft when in use, the rubber plate can better carry out the protection work when in fixation, the fixing effect on the animal tissue slice can be better, the displayed picture can be more stable, the detection effect on the animal tissue slice is better, and the detection stability and the detection comprehensiveness are better improved.
Furthermore, the flatness and the thickness of the animal tissue slices are obtained, the third motor is controlled to be started to lift the fixing mechanism and control the first motor to rotate the pressing plate according to the flatness and the thickness of the animal tissue slices, the control precision of the fixing mechanism is improved, the detection precision of the animal tissue slices is improved, the detection stability and the comprehensiveness of the animal tissue slices are improved, and the accuracy of detection results is further improved.
Further, through presetting roughness and preset thickness at the control unit to it is right with the comparison result of presetting the roughness and presetting thickness according to the roughness and the thickness of actual detection fixed establishment highly adjust and right the luminance of sensitization board is adjusted, has improved the control accuracy to fixed establishment, has improved the detection precision to animal tissue section simultaneously, has improved the detection stability and the comprehensive nature to animal tissue section, thereby has further improved the accuracy nature of testing result.
Furthermore, the control unit is provided with a preset flatness and a preset thickness range, the flatness and the thickness obtained by scanning and shooting images are compared with the preset flatness and the preset thickness range, whether the animal tissue slice is qualified or not is judged according to the comparison result, the detected ultrasonic wave length is determined according to the comparison result, the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
Particularly, a preset thickness difference value and a wavelength adjusting coefficient are set in the control module, when the actually acquired thickness is not in a preset thickness range, the difference value between the thickness and the minimum value of the preset thickness range or the maximum value of the preset thickness range is compared and calculated, and the corresponding adjusting coefficient is selected according to the comparison result of the difference value and the preset thickness difference value to adjust the preset ultrasonic wavelength, so that the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
Furthermore, the control unit is provided with a preset thickness and light source brightness, and the light source brightness is determined according to the comparison result of the thickness of the animal tissue slice actually detected and the preset thickness, so that the control precision of the brightness of the scanning mechanism is improved, and the accuracy of the detection result is further improved.
Further, through presetting roughness and preset thickness at the control unit to it is right with the comparison result of presetting the roughness and presetting thickness according to the roughness and the thickness of actual detection fixed establishment highly adjust and right the luminance of sensitization board is adjusted, has improved the control accuracy to fixed establishment, has improved the detection precision to animal tissue section simultaneously, has improved the detection stability and the comprehensive nature to animal tissue section, thereby has further improved the accuracy nature of testing result.
Drawings
FIG. 1 is a schematic front view of an image enhancement apparatus for imaging animal tissue slices according to the present invention;
FIG. 2 is a schematic diagram of a carriage structure of an image enhancement device based on animal tissue slice visualization according to the present invention;
fig. 3 is a schematic view of a buckle structure of the image enhancement device based on animal tissue slice visualization according to the present invention.
In the figure: 100. a carrying mechanism; 200. a fixing mechanism; 300. a scanning mechanism; 1. a slide base; 101. fixing the rod; 102. a base body; 103. a ball bearing; 104. a limiting plate; 2. a support plate; 3. a rotating shaft; 4. pressing a plate; 5. a rubber plate; 6. a clamping seat; 7. rotating the rod; 8. buckling; 801. reserving a hole; 802. a clamping plate; 803. a buckle body; 804. a hook; 9. a light-sensing plate; 10. a limiting groove; 11. a slideway; 12. a slide rail; 13. a base plate.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1-3, fig. 1 is a schematic front view of an image enhancement apparatus for imaging animal tissue slices according to the present invention; FIG. 2 is a schematic diagram of a carriage structure of an image enhancement device based on animal tissue slice visualization according to the present invention; fig. 3 is a schematic view of a buckle structure of the image enhancement device based on animal tissue slice visualization according to the present invention.
The invention relates to an image enhancement device based on animal tissue slice visualization, which comprises:
the bearing mechanism 100 comprises a bottom plate 13 and a light sensing plate 9 connected with the bottom plate 13, and is used for bearing the animal tissue slices;
the fixing mechanism 200 is connected with the bearing mechanism 100, the fixing mechanism 200 comprises a sliding seat 1 connected with one side of a bottom plate of the bearing mechanism 100, a supporting plate 2 connected with the sliding seat, a rotating shaft 3 connected with the supporting plate 2, a first motor (not shown in the figure) connected with one end of the rotating shaft 3 and a pressing plate 4 connected with the other end of the rotating shaft, and the fixing mechanism is used for fixing the animal tissue slice;
a scanning mechanism 300 connected to the fixing mechanism 200, a light source 14 for emitting a light beam to the light-sensing plate 9, a first camera 301 for detecting the position of the animal tissue slice, an ultrasonic scanner 303 for scanning the animal tissue slice to obtain an animal tissue image and slice flatness, and a second camera 302 for detecting the thickness of the animal tissue slice;
a second motor (not shown in the figure) for driving the fixing mechanism to move along the horizontal direction of the bottom plate and a third motor (not shown in the figure) for driving the fixing mechanism to move along the vertical direction of the bottom plate are arranged in the bottom plate, and a light transmission instrument (not shown in the figure) for detecting the light transmittance of the animal tissue section is also arranged in the bottom plate and close to the light sensing plate 9;
the bearing mechanism and the fixing mechanism are respectively connected with the control unit, and the control unit adjusts the rotation angle of the first motor in real time according to the placement position, the slice thickness and the slice flatness of the animal tissue slice so as to fix the animal tissue slice, the brightness of the light source and the height of the pressing plate so as to enhance the imaging image.
Specifically, the sliding seat 1 comprises a fixed rod 101, a seat body 102, two balls 103 and two limiting plates 104, the seat body 102 is fixedly connected to the lower end of the fixed rod 101, the limiting plates 104 are fixedly connected to the lower portion of the seat body 102, the balls 103 are movably connected to the inner portion of the limiting plates 104, the balls 103 are symmetrically distributed according to the axis of the limiting plates 104, a supporting plate 2 is fixedly connected to the upper portion of the sliding seat 1, a rotating shaft 3 is movably connected to one side of the supporting plate 2, a pressing plate 4 is movably connected to the outer wall of the rotating shaft 3, and a rubber plate 5 is fixedly connected to the lower portion of the pressing plate 4; during operation, through slide 1, dead lever 101, pedestal 102, ball 103, limiting plate 104, backup pad 2, pivot 3, cooperation work between clamp plate 4 and the rubber slab 5 lets equipment have better guarantee to animal tissue section fixed stability in the use, clamp plate 4 rotates through pivot 3 when using and lets clamp plate 4 drive rubber slab 5 that can be better carry out the fixed work, can be better through rubber slab 5 when fixed protect the work, can let the picture that shows better to the sliced fixed effect of animal tissue more stable during the use, it is better to the sliced detection effect of animal tissue, there is better promotion to detection stability and detection comprehensiveness.
Specifically, one end of the pressing plate 4 is fixedly connected with a clamping seat 6, the interior of the clamping seat 6 is movably connected with a rotating rod 7, the outer wall of the rotating rod 7 is movably connected with a buckle 8, the buckle 8 comprises a reserved hole 801, a clamping plate 802, a buckle body 803 and a hook 804, the reserved hole 801 is formed in the clamping plate 802, the buckle body 803 is fixedly connected below the clamping plate 802, and one side of the buckle body 803 is fixedly connected with the hook 804; the during operation, through fixed pressing plate 4 that buckle 8 can be better, let the fixed stability of clamp plate 4 have better promotion, can be better fix in the use, have more stable promotion to animal tissue slice's video picture effect.
Specifically, one side of the sliding seat 1 is movably connected with a sliding rail 12, the front of the sliding rail 12 is provided with a sliding way 11, and the lower inner wall of the sliding way 11 is provided with a limiting groove 10; during operation, through the slip between slide 1 and the slide rail 12, let the position of placing that can be better when using of equipment according to animal tissue section slide, let the shift position that clamp plate 4 can be better fix, the not unidimensional animal tissue section of adaptation that can be better when using.
Specifically, a bottom plate 13 is fixedly connected to the back of the slide rail 12, and a light-sensing plate 9 is fixedly connected to the upper side of the bottom plate 13; during operation, through the photosensitive plate 9 can be better to the demonstration luminance of equipment have better promotion, guarantee lighting condition that can be better when observing has better promotion to the observation effect.
Specifically, the pressing plate 4 is connected with the rubber plate 5 through gluing, and the pressing plate 4 and the supporting plate 2 form a rotating structure through the rotating shaft 3; during operation, the whole fixing stability of the device and the protection effect of the animal tissue slices during fixing are better promoted by the adhesive connection between the pressing plate 4 and the rubber plate 5.
Specifically, the sliding seat 1 is connected with the support plate 2 by welding, and the support plate 2 forms a sliding structure with the sliding rail 12 through the sliding seat 1; during operation, welded connection between the sliding seat 1 and the supporting plate 2 enables the fixing stability of the sliding seat 1 to be better improved, and stability of the supporting plate 2 during sliding is enabled to be better guaranteed.
Specifically, the buckle 8 forms a rotating structure through the rotating rod 7 and the clamping seat 6, and forms a penetrating structure between the rotating rod 7 and the buckle 8; during operation, buckle 8 lets clamp plate 4 can be better fix through buckle 8 through the revolution mechanic between swing arm 7 and block seat 6, guarantee animal tissue section's that can be better fixed stability when using.
Specifically, the seat body 102 and the limit plate 104 are connected by welding, and the ball 103 and the limit plate 104 form a fitting structure; during operation, the embedding structure between the balls 103 and the limiting plate 104 enables the equipment to better roll by means of the balls 103 when sliding, so as to provide lubrication for the sliding operation of the sliding base 1 and reduce friction force.
According to the image enhancement device based on animal tissue slice imaging, when the animal tissue slice is scanned, the control unit controls and starts the second motor and the ultrasonic scanner to sweep the light-sensitive plate, the position of the animal tissue slice is obtained when the sweeping is completed, when the position of the animal tissue slice is determined to be completed, the control unit controls the second motor to move the fixing mechanism to the position above the animal tissue slice, the animal tissue slice is scanned for the first time through the ultrasonic scanner to obtain a scanned image, and the animal tissue slice is shot through the second camera to obtain a shot image.
When the scanned image and the shot image are obtained, the control unit extracts the flatness and the thickness of the animal tissue slice from the scanned image and the shot image, controls and starts the third motor to lift the fixing mechanism to the height of the corresponding thickness of the animal tissue slice when the extraction is completed, and controls the first motor to rotate so that the pressing plate is attached to the upper surface of the animal tissue slice to fix the animal tissue slice, and the fixing stability is ensured under the condition of no damage to the slice.
When the control unit acquires the slice thickness and the slice flatness, whether the slice is qualified is judged according to the acquired thickness and flatness, and when the slice is qualified, the ultrasonic scanner is controlled to scan the animal tissue slice for the second time to acquire an ultrasonic image and control the optical image of the animal tissue on the light sensing plate, the control module combines the ultrasonic image and the optical image and then outputs the ultrasonic image and the optical image, so that the detection image of the animal tissue slice is acquired, and the imaging effect of the animal tissue slice is improved.
Particularly, the flatness and the thickness of the animal tissue slices are obtained, the third motor is controlled to be started to lift the fixing mechanism and control the first motor to rotate the pressing plate according to the flatness and the thickness of the animal tissue slices, the control precision of the fixing mechanism is improved, the detection precision of the animal tissue slices is improved, the detection stability and the comprehensiveness of the animal tissue slices are improved, and the accuracy of detection results is further improved.
The image enhancement device based on animal tissue slice visualization of the invention, when the animal tissue slice is scanned to obtain the visualization image, the control unit obtains the flatness U and the thickness H of the animal tissue slice according to the scanning image scanned by the ultrasonic scanner and the shooting image shot by the second camera, the control unit compares the flatness U with a preset flatness Uy and compares the thickness H with a preset thickness range H0, the control unit determines the ultrasonic wave length for scanning the animal tissue slice according to the comparison result of the flatness U, the preset flatness U0, the thickness H and the preset thickness range H0, wherein the preset thickness range H0 comprises a preset thickness range minimum Hmin and a preset thickness range maximum Hmax, and Hmin is set to be less than Hmax,
the control unit is also provided with a first preset ultrasonic wavelength lambda 1 and a second preset ultrasonic wavelength lambda 2, wherein lambda 1 is less than lambda 2;
if U is larger than or equal to Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a first preset ultrasonic wavelength lambda 1;
if U is less than Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a second preset ultrasonic wavelength lambda 2;
if U is not less than Uy and
Figure GDA0003595358620000121
the control unit respectively compares the thickness H with a preset minimum thickness range Hmin and a preset maximum thickness range Hmax;
if U < Uy and
Figure GDA0003595358620000122
the control unit determines that the animal tissue section is not qualified.
Specifically, when the control unit compares the thickness H with a preset thickness range minimum Hmin and a preset thickness range maximum Hmax, if H is less than Hmin, the control unit preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is a first preset ultrasonic wavelength lambda 1, calculates a first thickness difference value delta Ha between the thickness H and the preset thickness range minimum Hmin, selects a corresponding adjusting coefficient according to the comparison result of the first thickness difference value and the preset thickness difference value to adjust the ultrasonic length of the ultrasonic scanner,
wherein the control unit is also provided with a first preset thickness difference Delta H1, a second preset thickness difference Delta H2, a third preset thickness difference Delta H3, a first ultrasonic wave length adjustment coefficient K1, a second ultrasonic wave length adjustment coefficient K2 and a third ultrasonic wave length adjustment coefficient K3, wherein Delta H1 is more than Delta H2 and more than Delta H3,0.5 is more than K3 and more than K2 and more than K1 and more than 1,
when the delta H1 is more than or equal to the delta Ha and less than the delta H2, the control unit selects a first ultrasonic wave length adjusting coefficient K1 to adjust the first preset ultrasonic wave length lambda 1;
when the delta H2 is not less than the delta Ha and is less than the delta H3, the control unit selects a second ultrasonic wave length adjusting coefficient K2 to adjust the first preset ultrasonic wave length lambda 1;
when the delta Ha is larger than or equal to the delta H3, the control unit selects a third ultrasonic wave length adjusting coefficient K3 to adjust the first preset ultrasonic wave length lambda 1;
when the control unit selects the ith ultrasonic wave length adjusting coefficient Ki to adjust the first preset ultrasonic wave length, setting i to be 1, 2 and 3, and setting the adjusted first preset ultrasonic wave length lambda 1 to be lambda 1 'and setting lambda 1' to be lambda 1 multiplied by Ki by the control unit.
Specifically, if H is more than 1.5Hmax, the control unit judges that the animal tissue slice is unqualified, if Hmax is more than H and less than 1.5Hmax, the control unit preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is the second preset ultrasonic wavelength lambda 2, calculates the second thickness difference value delta Hb between the thickness H and the maximum value Hmax of the preset thickness range, selects a corresponding adjusting coefficient according to the comparison result of the second thickness difference value and the preset thickness difference value to adjust the ultrasonic length of the ultrasonic scanner,
wherein the control unit is also provided with a fourth ultrasonic wave length adjusting coefficient K4, a fifth ultrasonic wave length adjusting coefficient K5 and a sixth ultrasonic wave length adjusting coefficient K6, K4 is more than 1 and more than K4 and more than K6 and less than 2,
when the delta H1 is not less than the delta Hb and is less than the delta H2, the control unit selects a fourth ultrasonic wave length adjusting coefficient K4 to adjust the second preset ultrasonic wave length lambda 2;
when the delta H2 is not less than the delta Hb which is less than the delta H3, the control unit selects a fifth ultrasonic wave length adjusting coefficient K5 to adjust the second preset ultrasonic wave length lambda 2;
when the delta Hb is larger than or equal to the delta H3, the control unit selects a sixth ultrasonic wave length adjusting coefficient K6 to adjust the second preset ultrasonic wave length lambda 2;
when the control unit selects the jth ultrasonic wave length adjustment coefficient Kj to adjust the first preset ultrasonic wave length, setting j to be 4, 5 and 6, and setting the adjusted second preset ultrasonic wave length lambda 2 to be lambda 2 'and lambda 2' to be lambda 2 xkj by the control unit.
Specifically, the preset flatness and the preset thickness range are set in the control unit, the flatness and the thickness obtained by scanning the image and shooting the image are compared with the preset flatness and the preset thickness range, whether the animal tissue slice is qualified or not is judged according to the comparison result, the detected ultrasonic wave length is determined according to the comparison result, the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
Particularly, a preset thickness difference value and a wavelength adjusting coefficient are set in the control module, when the actually acquired thickness is not in a preset thickness range, the difference value between the thickness and the minimum value of the preset thickness range or the maximum value of the preset thickness range is compared and calculated, and the corresponding adjusting coefficient is selected according to the comparison result of the difference value and the preset thickness difference value to adjust the preset ultrasonic wavelength, so that the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
According to the image enhancement device based on animal tissue slice visualization, when the thickness extraction is completed, the control unit determines the brightness of the light-sensing plate according to the comparison result of the thickness and the preset thickness set in the control unit, and when the brightness of the light-sensing plate is determined to be completed, the brightness of the light-sensing plate is adjusted according to the comparison result of the flatness and the preset flatness set by the control unit.
Specifically, when the control unit controls to scan the animal tissue slice, the control unit preliminarily determines the brightness of the light source according to the comparison result of the thickness H of the animal tissue slice and the preset thickness,
wherein the control unit is also provided with a first preset thickness H1, a second preset thickness H2, a third preset thickness H3, a first light source brightness G1, a second light source brightness G2 and a third light source brightness G3, H1 is more than H2 is more than H3, G1 is more than G2 is more than G3,
when H1 is less than or equal to H < H2, the control unit sets the brightness of the light source to be the first light source brightness G1;
when H2 is less than or equal to H < H3, the control unit sets the brightness of the light source as second light source brightness G2;
when H is equal to or greater than H3, the control unit sets the brightness of the light source to be the third light source brightness G3.
Specifically, the control unit is provided with the preset thickness and the light source brightness, and the light source brightness is determined according to the comparison result of the thickness of the animal tissue slice detected actually and the preset thickness, so that the control precision of the brightness of the scanning mechanism is improved, and the accuracy of the detection result is further improved.
The image enhancement device based on animal tissue slice visualization is characterized in that when the pressing plate is attached to the upper surface of the animal tissue slice, the rotating angle of the first motor is finely adjusted according to the comparison result of the flatness and the preset flatness acquired by the control unit, and when the adjustment is completed, the control unit acquires the numerical value of the light transmission instrument.
Specifically, when the control unit controls the ultrasonic scanner to obtain the imaging image, the control unit obtains the preliminarily determined light source brightness G, if U is less than Uy and H belongs to H0, the control unit calculates the flatness difference value delta U between the flatness and the preset flatness U0, selects the corresponding brightness adjusting coefficient according to the comparison result of the flatness difference value and the preset flatness difference value to adjust the light source brightness,
wherein the control unit is further provided with a first preset flatness difference delta U1, a second preset flatness difference delta U2, a third preset flatness difference delta U3, a first brightness adjustment coefficient X1, a second brightness adjustment coefficient X2 and a third brightness adjustment coefficient X3, wherein delta U1 is more than delta U2 and less than delta U3,1 is more than X1 and more than X2 and more than X3 and less than 2,
when the delta U1 is not less than the delta U < delta U2, the control unit selects a first brightness adjusting coefficient X1 to adjust the brightness of the light source;
when the delta U2 is not less than the delta U < delta U3, the control unit selects a second brightness adjusting coefficient X2 to adjust the brightness of the light source;
when the delta U is larger than or equal to the delta U3, the control unit selects a third brightness adjusting coefficient X3 to adjust the brightness of the light source;
when the control unit selects the e-th brightness adjustment coefficient Xe to adjust the brightness of the light source, e is set to 1, 2, 3, and the control unit sets the adjusted brightness of the light source to G' which is set to G × Xe.
Particularly, through presetting roughness and preset thickness at the control unit to it is right with the comparison result of presetting the roughness and presetting thickness according to actual detection's roughness and thickness fixed establishment's highly adjusting and right the luminance of sensitization board is adjusted, has improved the control accuracy to fixed establishment, has improved the detection precision to animal tissue section simultaneously, has improved the detection stability and the comprehensive nature to animal tissue section, thereby has further improved the accuracy nature of testing result.
The image enhancement device based on animal tissue slice visualization comprises a control unit, wherein the control unit controls a light transmission instrument to move to a position corresponding to an animal tissue slice and obtains a numerical value of the light transmission instrument, the numerical value T of the light transmission instrument is compared with a preset light transmittance T0, if the T is more than or equal to T0, the control unit sets the light source brightness as the regulated light source brightness G', if the T is less than T0, the control unit calculates the light transmittance difference delta T between the numerical value T of the light transmission instrument and the preset light transmittance T0, and selects a corresponding brightness correction coefficient according to the comparison result of the light transmittance difference and the preset light transmittance difference to correct the light source brightness,
wherein the control unit is also provided with a first preset light transmittance difference Delta T1, a second preset light transmittance difference Delta T2, a third preset light transmittance difference Delta T3, a first brightness correction coefficient C1, a second brightness correction coefficient C2 and a third brightness correction coefficient C3, wherein Delta T1 is more than Delta T2 and more than Delta T3, 1 is more than C1 is more than C2 is more than C3 and less than 1.5,
when the delta T1 is not less than the delta T < delta T2, the control unit selects a first brightness correction coefficient C1 to correct the brightness of the light source;
when the delta T2 is not less than the delta T < delta T3, the control unit selects a third brightness correction coefficient C2 to correct the brightness of the light source;
when the delta T is larger than or equal to the delta T3, the control unit selects a third brightness correction coefficient C3 to correct the brightness of the light source;
when the control unit selects the n-th luminance correction coefficient Cn to correct the luminance of the light source, setting n ═ 1, 2, 3, the control unit sets the luminance of the light source after correction to G ' ″, setting G ' = G ' × Cn.
Specifically, the light transmittance difference value and the brightness correction coefficient are set in the control unit, and the corresponding brightness correction coefficient is selected according to the numerical value of the light transmission instrument and the comparison result of the light transmittance difference value of the preset light transmittance and the preset light transmittance difference value to correct the brightness of the light source, so that the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
The image enhancement apparatus based on animal tissue slice visualization of the invention, the control unit being further provided with a preset maximum light source luminance Gmax, the control unit comparing, when the control unit completes the light source luminance correction, the corrected light source luminance G ' ″ with the preset maximum light source luminance Gmax, the control unit calculating a luminance difference Δ G between the corrected light source luminance G ' ″ and the preset maximum light source luminance Gmax if G ' > Gmax, setting Δ G ' ═ G ' — Gmax, and selecting a corresponding correction coefficient to correct the second preset ultrasound wavelength based on a result of the comparison of the luminance difference with the preset luminance difference,
wherein the control unit is further provided with a first preset brightness difference value delta G1, a second preset brightness difference value delta G2, a third preset brightness difference value delta G3, a first wavelength correction coefficient W1, a second wavelength correction coefficient W2 and a third wavelength correction coefficient W3, wherein delta G1 < delta G2 < delta G3, 0.5 < W3 < W2 < W1 < 1 are set,
when the delta G1 is not less than delta G and is less than delta G2, the control unit selects a first wavelength correction coefficient W1 to correct the preset ultrasonic wavelength;
when the delta G2 is not less than delta G and is less than delta G3, the control unit selects a second wavelength correction coefficient W2 to correct the preset ultrasonic wavelength;
when the delta G is larger than or equal to the delta G3, the control unit selects a third wavelength correction coefficient W3 to correct the preset ultrasonic wavelength;
when the control unit selects the s-th wavelength correction coefficient Ws to correct the preset ultrasonic wavelength, s is set to 1, 2, 3, and the control unit sets the corrected ultrasonic wavelength to λ 3, and sets λ 3 to λ 1 '× Ws or λ 3 to λ 2' × Ws.
Specifically, the control module is provided with a preset maximum light source brightness, a preset brightness difference value and a wavelength correction coefficient, when the corrected light source brightness is larger than the preset maximum light source brightness, the brightness difference value between the light source brightness and the preset maximum light source brightness is calculated, and the control unit selects the corresponding correction coefficient according to the difference value to correct the ultrasonic wave length, so that the detection precision of the animal tissue slice is further improved, and the accuracy of the detection result is further improved.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention; various modifications and alterations to this invention will become apparent to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. An image enhancement device based on imaging of animal tissue slices, comprising:
the bearing mechanism is used for bearing the animal tissue slices and comprises a bottom plate and a light-sensitive plate connected with the bottom plate;
the fixing mechanism is connected with the bearing mechanism and used for fixing the animal tissue slices, and comprises a sliding seat connected with one side of a bottom plate of the bearing mechanism, a supporting plate connected with the sliding seat, a rotating shaft connected with the supporting plate, a first motor connected with one end of the rotating shaft and a pressing plate connected with the other end of the rotating shaft;
the scanning mechanism is connected with the fixing mechanism and comprises a light source used for emitting light beams to the light sensitive plate, a first camera used for detecting the position of the animal tissue slice, an ultrasonic scanner used for scanning the animal tissue slice to obtain an animal tissue imaging image and slice flatness and a second camera used for detecting the thickness of the animal tissue slice;
a second motor used for driving the fixing mechanism to move along the horizontal direction of the bottom plate and a third motor used for driving the fixing mechanism to move along the vertical direction of the bottom plate are arranged in the bottom plate, and a light transmission instrument used for detecting the light transmittance of the animal tissue slices is also arranged in the position, close to the light sensing plate, in the bottom plate;
bear mechanism, fixed establishment and scanning mechanism and be connected with the control unit respectively, it is right when animal tissue section scans, the control unit acquires animal tissue section place the position to when confirming to place the position and accomplish, acquire animal tissue section's section thickness and section roughness, and according to section thickness and section roughness in real time right the turned angle of first motor is adjusted in order to fix animal tissue section, the luminance of light source and the height of clamp plate are adjusted in order to strengthen the development image.
2. The image enhancement apparatus for animal tissue slice visualization according to claim 1, wherein when the animal tissue slice is scanned to obtain the visualization image, the control unit obtains the flatness U and the thickness H of the animal tissue slice according to the scan image scanned by the ultrasonic scanner and the shot image shot by the second camera, the control unit compares the flatness U with a preset flatness Uy and compares the thickness H with a preset thickness range H0, the control unit determines the ultrasonic length for scanning the animal tissue slice according to the comparison results of the flatness U and a preset flatness U0 and the thickness H and a preset thickness range H0, wherein the preset thickness range H0 comprises a preset thickness range minimum Hmin and a preset thickness range maximum Hmax, setting Hmin to be less than Hmax,
the control unit is also provided with a first preset ultrasonic wavelength lambda 1 and a second preset ultrasonic wavelength lambda 2, wherein lambda 1 is less than lambda 2;
if U is larger than or equal to Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a first preset ultrasonic wavelength lambda 1;
if U is less than Uy and H belongs to H0, the control unit controls the ultrasonic scanner to scan the animal tissue slice by a second preset ultrasonic wavelength lambda 2;
if U is larger than or equal to Uy and H ∉ H0, the control unit respectively compares the thickness H with a preset thickness range minimum value Hmin and a preset thickness range maximum value Hmax;
if U < Uy and H ∉ Hmax, the control unit determines that the animal tissue section is not qualified.
3. The image enhancement apparatus for imaging based on animal tissue slices according to claim 2, wherein when the control unit compares the thickness H with a preset minimum thickness range Hmin and a preset maximum thickness range Hmax, if H < Hmin, the control unit preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is a first preset ultrasonic wavelength λ 1, calculates a first thickness difference Δ Ha between the thickness H and the preset minimum thickness range Hmin, and selects a corresponding adjustment coefficient to adjust the ultrasonic wavelength of the ultrasonic scanner according to the comparison result between the first thickness difference and the preset thickness difference,
wherein the control unit is also provided with a first preset thickness difference Delta H1, a second preset thickness difference Delta H2, a third preset thickness difference Delta H3, a first ultrasonic wave length adjustment coefficient K1, a second ultrasonic wave length adjustment coefficient K2 and a third ultrasonic wave length adjustment coefficient K3, wherein Delta H1 is more than Delta H2 and more than Delta H3,0.5 is more than K3 and more than K2 and more than K1 and more than 1,
when the delta H1 is more than or equal to the delta Ha and less than the delta H2, the control unit selects a first ultrasonic wave length adjusting coefficient K1 to adjust the first preset ultrasonic wave length lambda 1;
when the delta H2 is more than or equal to the delta Ha and less than the delta H3, the control unit selects a second ultrasonic wave length adjusting coefficient K2 to adjust the first preset ultrasonic wave length lambda 1;
when the delta Ha is larger than or equal to the delta H3, the control unit selects a third ultrasonic wave length adjusting coefficient K3 to adjust the first preset ultrasonic wave length lambda 1;
when the control unit selects the ith ultrasonic wave length adjustment coefficient Ki to adjust the first preset ultrasonic wave length, i =1, 2, 3 is set, the control unit sets the adjusted first preset ultrasonic wave length lambda 1 as lambda 1 ', and sets lambda 1' = lambda 1 × Ki.
4. The image enhancement apparatus for imaging-based animal tissue slices according to claim 3, wherein the control unit determines that the animal tissue slice is not qualified if H > 1.5Hmax, and preliminarily determines that the ultrasonic wavelength of the ultrasonic scanner is the second preset ultrasonic wavelength λ 2 if Hmax < H < 1.5Hmax, calculates a second thickness difference Δ Hb between the thickness H and the maximum value Hmax of the preset thickness range, and selects a corresponding adjustment coefficient to adjust the ultrasonic wavelength of the ultrasonic scanner according to the comparison result between the second thickness difference and the preset thickness difference,
wherein the control unit is also provided with a fourth ultrasonic wave length adjusting coefficient K4, a fifth ultrasonic wave length adjusting coefficient K5 and a sixth ultrasonic wave length adjusting coefficient K6, wherein K4 is more than 1 and K4 is more than 1 and K6 is more than 2,
when the delta H1 is not less than the delta Hb which is less than the delta H2, the control unit selects a fourth ultrasonic wave length adjusting coefficient K4 to adjust the second preset ultrasonic wave length lambda 2;
when the delta H2 is not less than the delta Hb and is less than the delta H3, the control unit selects a fifth ultrasonic wave length adjusting coefficient K5 to adjust the second preset ultrasonic wave length lambda 2;
when the delta Hb is larger than or equal to the delta H3, the control unit selects a sixth ultrasonic wave length adjusting coefficient K6 to adjust the second preset ultrasonic wave length lambda 2;
when the control unit selects the jth ultrasonic wave length adjustment coefficient Kj to adjust the first preset ultrasonic wave length, j =4, 5, 6 is set, the control unit sets the adjusted second preset ultrasonic wave length lambda 2 to be lambda 2 ', and sets lambda 2' = lambda 2 × Kj.
5. The image enhancement device based on animal tissue slice visualization of claim 4, wherein when the control unit controls the ultrasonic scanner to obtain the visualization image, the control unit obtains a preliminarily determined light source brightness G, if U < Uy and H e is H0, the control unit calculates a flatness difference value Δ U between the flatness and a preset flatness U0, and selects a corresponding brightness adjustment coefficient to adjust the light source brightness according to the comparison result between the flatness difference value and the preset flatness difference value,
wherein the control unit is further provided with a first preset flatness difference delta U1, a second preset flatness difference delta U2, a third preset flatness difference delta U3, a first brightness adjustment coefficient X1, a second brightness adjustment coefficient X2 and a third brightness adjustment coefficient X3, wherein delta U1 is more than delta U2 and less than delta U3,1 is more than X1 and more than X2 and more than X3 and less than 2,
when the delta U1 is not less than the delta U < delta U2, the control unit selects a first brightness adjusting coefficient X1 to adjust the brightness of the light source;
when the delta U2 is not less than the delta U < delta U3, the control unit selects a second brightness adjusting coefficient X2 to adjust the brightness of the light source;
when the delta U is larger than or equal to the delta U3, the control unit selects a third brightness adjusting coefficient X3 to adjust the brightness of the light source;
when the control unit selects the e-th brightness adjustment coefficient Xe to adjust the brightness of the light source, e =1, 2, 3 is set, and the control unit sets the adjusted brightness of the light source to G 'and sets G' = G × Xe.
6. The animal tissue slice visualization-based image enhancement device of claim 5, wherein when the control unit controls the light transmission instrument to move to a position corresponding to the animal tissue slice and obtains a value of the light transmission instrument, and compares the value T of the light transmission instrument with a preset light transmittance T0, if T is greater than or equal to T0, the control unit sets the light source brightness as the adjusted light source brightness G', if T is less than T0, the control unit calculates a light transmittance difference Δ T between the value T of the light transmission instrument and the preset light transmittance T0, and selects a corresponding brightness correction coefficient to correct the light source brightness according to a comparison result of the light transmittance difference and the preset light transmittance difference,
wherein the control unit is further provided with a first preset light transmittance difference delta T1, a second preset light transmittance difference delta T2, a third preset light transmittance difference delta T3, a first brightness correction coefficient C1, a second brightness correction coefficient C2 and a third brightness correction coefficient C3, wherein delta T1 is more than delta T2 and is more than delta T3, 1 is more than C1 and is more than C2 and is more than C3 and is less than 1.5,
when the delta T1 is not less than the delta T < delta T2, the control unit selects a first brightness correction coefficient C1 to correct the brightness of the light source;
when the delta T2 is not less than the delta T < delta T3, the control unit selects a third brightness correction coefficient C2 to correct the brightness of the light source;
when the delta T is larger than or equal to the delta T3, the control unit selects a third brightness correction coefficient C3 to correct the brightness of the light source;
when the control unit selects the n-th luminance correction coefficient Cn to correct the luminance of the light source, n =1, 2, 3 is set, and the control unit sets the corrected luminance of the light source to G ' ″ and sets G ' = G ' xcn Cn.
7. The animal tissue slice visualization-based image enhancement apparatus of claim 6, wherein when the control unit controls the scanning of the animal tissue slice, the control unit preliminarily determines the brightness of the light source according to a comparison result of a thickness H of the animal tissue slice with a preset thickness,
wherein the control unit is also provided with a first preset thickness H1, a second preset thickness H2, a third preset thickness H3, a first light source brightness G1, a second light source brightness G2 and a third light source brightness G3, H1 is more than H2 is more than H3, G1 is more than G2 is more than G3,
when H1 is less than or equal to H < H2, the control unit sets the brightness of the light source to be the first light source brightness G1;
when H2 is less than or equal to H < H3, the control unit sets the brightness of the light source to be the second light source brightness G2;
when H is equal to or greater than H3, the control unit sets the brightness of the light source to be the third light source brightness G3.
8. The animal tissue slice visualization-based image enhancement apparatus according to claim 7, wherein the control unit is further provided with a preset maximum light source luminance Gmax, when the control unit finishes the light source luminance correction, the control unit compares a corrected light source luminance G '″ with the preset maximum light source luminance Gmax, if G' > Gmax, the control unit calculates a luminance difference Δ G between the corrected light source luminance G '″ and the preset maximum light source luminance Gmax, sets Δ G = G'/Gmax, and selects a corresponding correction coefficient to correct the second preset ultrasound wavelength based on a result of the comparison of the luminance difference with the preset luminance difference,
wherein the control unit is further provided with a first preset brightness difference value delta G1, a second preset brightness difference value delta G2, a third preset brightness difference value delta G3, a first wavelength correction coefficient W1, a second wavelength correction coefficient W2 and a third wavelength correction coefficient W3, wherein delta G1 < delta G2 < delta G3, 0.5 < W3 < W2 < W1 < 1 are set,
when the delta G1 is not less than delta G and is less than delta G2, the control unit selects a first wavelength correction coefficient W1 to correct the preset ultrasonic wavelength;
when the delta G2 is not less than the delta G which is less than the delta G3, the control unit selects a second wavelength correction coefficient W2 to correct the preset ultrasonic wavelength;
when the delta G is larger than or equal to the delta G3, the control unit selects a third wavelength correction coefficient W3 to correct the preset ultrasonic wavelength;
when the control unit selects the s-th wavelength correction coefficient Ws to correct the preset ultrasonic wave length, s =1, 2, 3 is set, and the control unit sets the corrected ultrasonic wave length to λ 3, and sets λ 3= λ 1 'xws or λ 3= λ 2' xws.
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