CN113655057A - Equipment for detecting influence of freeze drying treatment on vaccinia virus virulence - Google Patents
Equipment for detecting influence of freeze drying treatment on vaccinia virus virulence Download PDFInfo
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- CN113655057A CN113655057A CN202110976688.7A CN202110976688A CN113655057A CN 113655057 A CN113655057 A CN 113655057A CN 202110976688 A CN202110976688 A CN 202110976688A CN 113655057 A CN113655057 A CN 113655057A
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- freeze
- drying
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- video data
- observation
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- 238000004108 freeze drying Methods 0.000 title claims abstract description 101
- 241000700618 Vaccinia virus Species 0.000 title claims abstract description 25
- 230000001018 virulence Effects 0.000 title claims description 10
- 230000007246 mechanism Effects 0.000 claims abstract description 154
- 238000012545 processing Methods 0.000 claims abstract description 48
- 239000000523 sample Substances 0.000 claims description 84
- 230000000694 effects Effects 0.000 claims description 11
- 230000001681 protective effect Effects 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 15
- 230000001988 toxicity Effects 0.000 abstract description 4
- 231100000419 toxicity Toxicity 0.000 abstract description 4
- 230000008859 change Effects 0.000 description 9
- 239000013589 supplement Substances 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000001502 supplementing effect Effects 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 208000007089 vaccinia Diseases 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The invention discloses a device for detecting the influence of freeze drying on the toxicity of vaccinia virus, which comprises a detection device body, a sample placing platform, a freeze drying processing mechanism, a sample observation mechanism, a video data recording mechanism, a video data processing mechanism and a freeze drying fine adjustment mechanism, wherein the bottom end of the detection device body is provided with the freeze drying processing mechanism, the top end of the freeze drying processing mechanism is provided with the sample placing platform, the top end of the sample placing platform is provided with the sample observation mechanism, the side surface of the sample observation mechanism is provided with the video data recording mechanism, the side surface of the sample observation mechanism is provided with the video data processing mechanism, and the top end of the side surface of the freeze drying processing mechanism is provided with the freeze drying fine adjustment mechanism. Compared with the traditional observation mode, the working pressure of experimenters is greatly reduced.
Description
Technical Field
The invention relates to the technical field of electronic observation and detection equipment, in particular to equipment for detecting the influence of freeze drying treatment on the toxicity of vaccinia virus.
Background
The vaccinia vaccine inoculated rabbit inflammation skin extract can be used as a medicine for development, non-protein active ingredients with analgesic and antiallergic effects are separated from inflammation tissues of the vaccinia vaccine inoculated rabbits, long-time observation of vaccinia virus needs to be carried out through professional equipment for detecting and verifying activity change of the vaccinia virus under extreme conditions, traditional intermittent visual observation is adopted, key detection conditions and optimal observation visual angles are easily missed, only a single detection result can be obtained, activity change conditions of the vaccinia virus under the condition of freeze drying cannot be detected, and therefore how to effectively solve the problem is a problem which is urgently needed to be solved at present.
Disclosure of Invention
The invention aims to provide equipment for detecting the influence of freeze drying treatment on the toxicity of vaccinia virus, which is simple in structure and convenient to operate and use, can help experimenters to record the change of a virus sample under extreme conditions, can realize manual micro-adjustment and electronic observation, analysis and recording, and greatly reduces the working pressure of the experimenters compared with the traditional observation mode.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a detect freeze-drying and handle equipment to influence of vaccinia virus virulence includes the check out test set body, sample placement platform, freeze-drying processing mechanism, sample observation mechanism, video data record mechanism, video data processing mechanism, freeze-drying fine adjustment mechanism, check out test set body bottom is provided with freeze-drying processing mechanism, freeze-drying processing mechanism top is provided with sample placement platform, sample placement platform top is provided with sample observation mechanism, sample observation mechanism side is provided with video data record mechanism, sample observation mechanism side is provided with video data processing mechanism, freeze-drying processing mechanism side top is provided with freeze-drying fine adjustment mechanism.
As preferred, the sample place the platform bottom is provided with freeze drying contact port, freeze drying contact port top is provided with place the platform lifting sleeve, place the platform lifting sleeve side bottom is provided with the lift drive side tooth, place the platform lifting sleeve top is provided with the sample and places the square trough, the sample is placed the square trough both ends and is provided with the sample and places the access port, place the platform lifting sleeve top side is provided with sample square trough light filling mechanism, sample square trough light filling mechanism top is provided with light regulating switch.
Preferably, a treatment mechanism protective cover is arranged outside the freeze drying treatment mechanism, a circulating freeze treatment mechanism is arranged at one end of the inner side of the treatment mechanism protective cover, a circulating dry treatment mechanism is arranged at the other end of the inner side of the treatment mechanism protective cover, a freeze drying output conduit is arranged at the top end of the top of the freeze drying treatment mechanism, and a freeze drying regulation port is arranged on the side surface of the freeze drying output conduit.
Preferably, the bottom end of the sample observation mechanism is provided with a sample observation high power lens, the side surface of the sample observation mechanism is provided with an observation direction adjusting bracket, the top end of the sample observation mechanism is provided with a short-distance observation end, and the side surface of the sample observation mechanism is provided with a recording mechanism fixing hole.
Preferably, one end of the video data recording mechanism is provided with a video recording deep probe, the other end of the video recording deep probe is provided with an auxiliary fixed switching end head, and the inner side of the auxiliary fixed switching end head is provided with a video electric signal conversion mechanism.
Preferably, one end of the video data processing mechanism is provided with a video signal switching cable, the other end of the video data processing mechanism is provided with a video data display mechanism, the side face of the bottom end of the video data display mechanism is provided with an observation adjusting rocker, and the side face of the video data display mechanism is provided with a video data export port.
Preferably, one side of the freeze-drying fine adjustment mechanism is provided with a freeze-drying air speed adjusting baffle, the outer side of the freeze-drying air speed adjusting baffle is connected with a freeze-drying output conduit, and the other side of the freeze-drying fine adjustment mechanism is provided with a freeze-drying manual adjustment panel.
Compared with the prior art, the invention has the beneficial effects that:
(1) the sample placing platform can be used for placing a detection sample, the freeze-drying contact port can be used for contacting freeze-drying equipment, so that indirect freeze-drying treatment of the sample is realized, the direct contact and virus cross infection leakage conditions are avoided, the placing platform lifting sleeve can be matched with the sample placing square groove to realize free lifting, so that closed contact of the freeze-drying equipment is realized, the detection sample can be better protected, and the sample square groove light supplementing mechanism can be used for appropriately supplementing light to the sample for detection and observation.
(2) The freeze drying mechanism can provide a special environment for possible detection of vaccinia virus, so as to obtain the activity change data of the vaccinia virus under extreme conditions.
(3) The sample observation mechanism can realize direct naked eye observation and auxiliary electronic observation record, and the observation direction adjusting support can adjust the observation angle to a certain extent, so that the observation angle is prevented from being too single to obtain inaccurate data.
(4) The video data recording mechanism can convert observed light into data and perform system analysis to obtain physiological changes and activity changes of the vaccinia virus in different environments, and the system can conveniently make accurate detection data.
(5) Video data processing mechanism can realize showing and drawing the active figure that changes observation data, makes things convenient for the data that the professional reachs according to video and analysis to verify and confirm, can more audio-visually show the vaccinia virus sample change under freeze-drying's environment simultaneously, and the person's of facilitating the use later stage is examined and is observed.
(6) The freeze-drying fine adjustment mechanism can change the freeze-drying environment through manual adjustment, and the freeze-drying air speed adjusting baffle can realize the adjustment control of the actual condition of freeze-drying through controlling the turnover angle of the baffle, so that the actual operation control of the detection observer is greatly facilitated.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a sample placement platform according to the present invention;
FIG. 3 is a schematic view of the structure of the freeze drying mechanism of the present invention;
FIG. 4 is a schematic view of a sample observation mechanism according to the present invention;
FIG. 5 is a schematic view of a video data recording mechanism according to the present invention;
FIG. 6 is a schematic diagram of a video data processing mechanism according to the present invention;
FIG. 7 is a schematic view of the freeze drying micro-adjustment mechanism of the present invention.
In the figure: 1. detecting an equipment body; 2. a sample placement platform; 3. a freeze-drying processing mechanism; 4. a sample observation mechanism; 5. a video data recording mechanism; 6. a video data processing mechanism; 7. a freeze drying micro-adjustment mechanism; 8. freeze drying the contact port; 9. placing a platform lifting sleeve; 10. lifting the driving side teeth; 11. a sample placing square groove; 12. a sample placement in-out port; 13. a sample square groove light supplementing mechanism; 14. a light ray adjusting switch; 15. the processing mechanism protects the housing; 16. a circulating freezing treatment mechanism; 17. a circulating drying treatment mechanism; 18. a freeze-drying output conduit; 19. a freeze drying conditioning port; 20. observing a high power lens by a sample; 21. an observation direction adjusting bracket; 22. observing the end head at a short distance; 23. a recording mechanism fixing hole; 24. video recording is carried out to enter the probe; 25. auxiliary fixing of the adapter end; 26. a video electric signal conversion mechanism; 27. a video signal transfer cable; 28. a video data display mechanism; 29. observing and adjusting the rocker; 30. a video data export port; 31. a freeze-drying air speed regulating baffle plate; 32. freeze-drying the panels were adjusted manually.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
As shown in figure 1, the device for detecting the influence of freeze drying on the toxicity of vaccinia virus comprises a detection device body 1, a sample placement platform 2, a freeze drying processing mechanism 3, a sample observation mechanism 4, a video data recording mechanism 5, a video data processing mechanism 6 and a freeze drying fine adjustment mechanism 7, wherein the freeze drying processing mechanism 3 is arranged at the bottom end of the detection device body 1, the sample placement platform 2 is arranged at the top end of the freeze drying processing mechanism 3, the sample observation mechanism 4 is arranged at the top end of the sample placement platform 2, the video data recording mechanism 5 is arranged on the side surface of the sample observation mechanism 4, the video data processing mechanism 6 is arranged on the side surface of the sample observation mechanism 4, and the freeze drying fine adjustment mechanism 7 is arranged on the side surface top end of the freeze drying processing mechanism 3.
As shown in fig. 2, the bottom end of the sample placement platform 2 is provided with a freeze-drying contact port 8, the top end of the freeze-drying contact port 8 is provided with a placement platform lifting sleeve 9, the bottom end of the side surface of the placement platform lifting sleeve 9 is provided with a lifting driving side tooth 10, the top end of the placement platform lifting sleeve 9 is provided with a sample placement square groove 11, the two ends of the sample placement square groove 11 are provided with a sample placement inlet and outlet port 12, the side surface of the top end of the placement platform lifting sleeve 9 is provided with a sample square groove light supplement mechanism 13, and the top end of the sample square groove light supplement mechanism 13 is provided with a light adjusting switch 14.
As shown in fig. 3, a processing mechanism protective cover 15 is disposed outside the freeze-drying processing mechanism 3, a circulating freeze-drying processing mechanism 16 is disposed at one end of the inner side of the processing mechanism protective cover 15, a circulating dry-processing mechanism 17 is disposed at the other end of the inner side of the processing mechanism protective cover 15, a freeze-drying output conduit 18 is disposed at the top end of the top of the freeze-drying processing mechanism 3, and a freeze-drying regulation port 19 is disposed at the side surface of the freeze-drying output conduit 18.
As shown in fig. 4, a specimen observation high power lens 20 is disposed at the bottom end of the specimen observation mechanism 4, an observation direction adjusting bracket 21 is disposed at the side surface of the specimen observation mechanism 4, a short-distance observation end 22 is disposed at the top end of the specimen observation mechanism 4, and a recording mechanism fixing hole 23 is disposed at the side surface of the specimen observation mechanism 4.
As shown in fig. 5, one end of the video data recording mechanism 5 is provided with a video recording deep probe 24, the other end of the video recording deep probe 24 is provided with an auxiliary fixed switching end 25, and the inner side of the auxiliary fixed switching end 25 is provided with a video electric signal conversion mechanism 26.
As shown in fig. 6, one end of the video data processing mechanism 6 is provided with a video signal transfer cable 27, the other end of the video data processing mechanism 6 is provided with a video data display mechanism 28, the side surface of the bottom end of the video data display mechanism 28 is provided with an observation adjusting rocker 29, and the side surface of the video data display mechanism 28 is provided with a video data export port 30.
As shown in fig. 7, a freeze-drying air velocity adjusting baffle 31 is provided on one side of the freeze-drying fine adjustment mechanism 7, the freeze-drying output duct 18 is connected to the outer side of the freeze-drying air velocity adjusting baffle 31, and a manual freeze-drying adjustment panel 32 is provided on the other side of the freeze-drying fine adjustment mechanism 7.
Application method
The method comprises the steps that a user firstly places a detected vaccinia virus in a sealed culture medium, a sample placing square groove 11 is extended out through a placing platform lifting sleeve 9, the sealed culture medium is placed into the sample placing square groove 11 through a sample placing inlet and outlet port 12, a sample square groove light supplement mechanism 13 is opened to supplement light for the detected sample, the sample placing square groove 11 is led in through the placing platform lifting sleeve 9, freezing and drying adjustment is firstly carried out on a freeze drying processing mechanism 3 through a freeze drying micro-adjustment mechanism 7, the sample is observed through a sample observation mechanism 4, the change of the vaccinia virus is observed while the temperature and the drying environment are adjusted, finally, the virus sample is recorded and observed for a long time through a video data recording mechanism 5, the change of the vaccinia virus under the freeze drying change environment is drawn through a video data processing mechanism 6, and corresponding detection data are obtained, and reversely operating to take out the vaccinia virus sample, and properly processing, so that the detection equipment body 1 is used by one operation.
The above embodiments are only preferred embodiments of the present invention, and are not intended to limit the technical solutions of the present invention, so long as the technical solutions can be realized on the basis of the above embodiments without creative efforts, which should be considered to fall within the protection scope of the patent of the present invention.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. An apparatus for detecting the effect of freeze-drying on vaccinia virus virulence, comprising: including check out test set body (1), sample place the platform (2), freeze drying processing mechanism (3), sample observation mechanism (4), video data record mechanism (5), video data processing mechanism (6), freeze-drying fine-tuning mechanism (7), check out test set body (1) bottom is provided with freeze drying processing mechanism (3), freeze drying processing mechanism (3) top is provided with sample place the platform (2), sample place the platform (2) top is provided with sample observation mechanism (4), sample observation mechanism (4) side is provided with video data record mechanism (5), sample observation mechanism (4) side is provided with video data processing mechanism (6), freeze drying processing mechanism (3) side top is provided with freeze-drying fine-tuning mechanism (7).
2. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: sample place platform (2) bottom is provided with freeze-drying contact port (8), freeze-drying contact port (8) top is provided with place platform lifting sleeve (9), place platform lifting sleeve (9) side bottom is provided with lift drive side tooth (10), place platform lifting sleeve (9) top is provided with sample and places square groove (11), sample is placed square groove (11) both ends and is provided with the sample and places inlet and outlet port (12), place platform lifting sleeve (9) top side is provided with sample square groove light filling mechanism (13), sample square groove light filling mechanism (13) top is provided with light regulating switch (14).
3. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: the freeze-drying treatment mechanism (3) outside is provided with processing mechanism protective housing (15), the inboard one end of processing mechanism protective housing (15) is provided with circulation freeze treatment mechanism (16), the inboard other end of processing mechanism protective housing (15) is provided with circulation dry treatment mechanism (17), freeze-drying treatment mechanism (3) top is provided with freeze-drying output pipe (18), freeze-drying output pipe (18) side is provided with freeze-drying regulation port (19).
4. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: the device is characterized in that a sample observation high power lens (20) is arranged at the bottom end of the sample observation mechanism (4), an observation direction adjusting support (21) is arranged on the side surface of the sample observation mechanism (4), a near observation end (22) is arranged at the top end of the sample observation mechanism (4), and a recording mechanism fixing hole (23) is arranged on the side surface of the sample observation mechanism (4).
5. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: one end of the video data recording mechanism (5) is provided with a video recording deep probe (24), the other end of the video recording deep probe (24) is provided with an auxiliary fixed switching end head (25), and the inner side of the auxiliary fixed switching end head (25) is provided with a video electric signal conversion mechanism (26).
6. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: video signal switching cable (27) is arranged at one end of the video data processing mechanism (6), a video data display mechanism (28) is arranged at the other end of the video data processing mechanism (6), an observation adjusting rocker (29) is arranged on the side surface of the bottom end of the video data display mechanism (28), and a video data export port (30) is arranged on the side surface of the video data display mechanism (28).
7. The apparatus of claim 1, wherein the apparatus is configured to detect the effect of freeze-drying on the virulence of a vaccinia virus: freeze-drying wind speed adjusting baffle (31) is provided with on one side of freeze-drying fine adjustment mechanism (7), freeze-drying output pipe (18) is connected in freeze-drying wind speed adjusting baffle (31) outside, freeze-drying fine adjustment mechanism (7) opposite side is provided with manual adjusting panel of freeze-drying (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110976688.7A CN113655057A (en) | 2021-08-24 | 2021-08-24 | Equipment for detecting influence of freeze drying treatment on vaccinia virus virulence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110976688.7A CN113655057A (en) | 2021-08-24 | 2021-08-24 | Equipment for detecting influence of freeze drying treatment on vaccinia virus virulence |
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| CN113655057A true CN113655057A (en) | 2021-11-16 |
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Citations (7)
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|---|---|---|---|---|
| JPH07198714A (en) * | 1993-12-28 | 1995-08-01 | Asahi Breweries Ltd | Method and device for discriminating activity of cell |
| CN1818738A (en) * | 2006-03-10 | 2006-08-16 | 浙江大学 | Microscope observation system during freezing dry process |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| CN101636486A (en) * | 2007-03-19 | 2010-01-27 | 稳定技术有限公司 | Preserve the method for virion |
| CN108395986A (en) * | 2018-03-23 | 2018-08-14 | 余晖 | Human papilloma virus automatic parting direction detection device based on deep learning |
| CN111238185A (en) * | 2020-01-19 | 2020-06-05 | 陕西科技大学 | Device for monitoring microwave vacuum drying |
| CN112088874A (en) * | 2020-09-24 | 2020-12-18 | 陈龙刚 | Stem cell cold storage cooling device |
-
2021
- 2021-08-24 CN CN202110976688.7A patent/CN113655057A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07198714A (en) * | 1993-12-28 | 1995-08-01 | Asahi Breweries Ltd | Method and device for discriminating activity of cell |
| CN1818738A (en) * | 2006-03-10 | 2006-08-16 | 浙江大学 | Microscope observation system during freezing dry process |
| CN101636486A (en) * | 2007-03-19 | 2010-01-27 | 稳定技术有限公司 | Preserve the method for virion |
| CN101231249A (en) * | 2008-02-26 | 2008-07-30 | 东北大学 | Vacuum low temperature microscopic visualizer |
| CN108395986A (en) * | 2018-03-23 | 2018-08-14 | 余晖 | Human papilloma virus automatic parting direction detection device based on deep learning |
| CN111238185A (en) * | 2020-01-19 | 2020-06-05 | 陕西科技大学 | Device for monitoring microwave vacuum drying |
| CN112088874A (en) * | 2020-09-24 | 2020-12-18 | 陈龙刚 | Stem cell cold storage cooling device |
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