CN113648276A - Preparation method of serum protein complete culture solution of mesenchymal cells and application of serum protein complete culture solution in cosmetics - Google Patents
Preparation method of serum protein complete culture solution of mesenchymal cells and application of serum protein complete culture solution in cosmetics Download PDFInfo
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- CN113648276A CN113648276A CN202111064363.8A CN202111064363A CN113648276A CN 113648276 A CN113648276 A CN 113648276A CN 202111064363 A CN202111064363 A CN 202111064363A CN 113648276 A CN113648276 A CN 113648276A
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- 238000000034 method Methods 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims description 52
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 14
- 102000008100 Human Serum Albumin Human genes 0.000 claims description 11
- 108091006905 Human Serum Albumin Proteins 0.000 claims description 11
- 102000016359 Fibronectins Human genes 0.000 claims description 10
- 108010067306 Fibronectins Proteins 0.000 claims description 10
- 101000976075 Homo sapiens Insulin Proteins 0.000 claims description 10
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 10
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- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 10
- 229960001669 kinetin Drugs 0.000 claims description 10
- 229960001471 sodium selenite Drugs 0.000 claims description 10
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- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims description 8
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- 229920001503 Glucan Polymers 0.000 claims description 7
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
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- 206010014970 Ephelides Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
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- Gerontology & Geriatric Medicine (AREA)
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- Cosmetics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a preparation method of a serum protein complete culture solution of mesenchymal cells and application of the serum protein complete culture solution in cosmetics. The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method of the serum protein complete culture solution of the mesenchymal cells can be applied to cosmetics, and nutrient components in the serum protein complete culture solution of the mesenchymal cells and cytokines generated by the mesenchymal cells in the proliferation growth process can be used for effectively supplementing nutrient repairing components to the skin cells, reducing the inflammation condition of the skin cells, slowing down the aging and apoptosis of the cells, and promoting the metabolism and proliferation growth of the cells, so that the damaged skin is repaired, the health state of the skin is recovered, the skin is smoother and more delicate, a new multi-effect cosmetic is provided for consumers, and the popularization prospect is good.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a preparation method of a serum protein complete culture solution of mesenchymal cells and application of the serum protein complete culture solution in cosmetics.
Background
The mesenchymal stem cells are early undifferentiated cells, have the characteristics of self-renewal, self-replication, unlimited proliferation, multidirectional differentiation potential and the like, can reduce inflammation, reduce apoptosis of tissue cells, promote proliferation of stem progenitor cells of endogenous tissues and organs and carry out immune regulation by secreting cell factors, and thus, the mesenchymal stem cells serve as seed cells to achieve the effect of repairing the tissues and organs. The cells still have multidirectional differentiation potential after continuous subculture and cryopreservation, and are called as universal cells in the medical field.
And the cell factors can be secreted in the process of culturing the mesenchymal stem cells, so the cell factors also exist in the culture solution, and the cosmetic industry for beautifying the life of people is rapidly developed along with the continuous improvement of the spiritual civilization and the material living standard of people. In particular, cosmetics for moisturizing, anti-aging, whitening and removing freckles are increasingly popular with consumers as the mainstream of a plurality of beauty and skin care products in recent years. Skin aging is a continuous and progressive physiological process that directly affects the appearance and function of skin. Wrinkles are a sign of the appearance of skin aging and are called "annual ring of life". The cell factor secreted by the mesenchymal stem cell can be used in cosmetics to promote the metabolism of skin cells and slow down the aging of the cells.
Disclosure of Invention
In view of the problems in the prior art, the invention discloses a preparation method of a serum protein complete culture solution of mesenchymal cells, which comprises the following steps:
selecting DMEM as a basic culture medium, and preparing main components according to concentration ratio requirements, wherein the main components comprise human serum albumin, recombinant human insulin, fibronectin, L-glutamine, sodium selenite, epidermal growth factors, kinetin and glucan;
step two, uniformly mixing the main components prepared in the step one, then selecting a filter membrane with the aperture of 0.1-0.5 mu m for filtration and sterilization to obtain a culture solution stock solution of the mesenchymal cells, and putting the culture solution stock solution into a culture dish;
step three, inoculating the mesenchymal cells into a culture dish, and culturing the mesenchymal cells;
and step four, filtering the culture solution in the culture dish when the cells grow to 40-60% in a confluent manner, and removing the mesenchymal cells to obtain the serum protein complete culture solution of the mesenchymal cells.
As a preferable scheme of the invention, in the step one, the concentration ratio of the main components is as follows: 4-10mg/mL of human serum albumin, 0.1-1mg/mL of recombinant human insulin, 10-20 mu g/mL of fibronectin, 2-12mM of L-glutamine, 0.2-0.7mg/mL of sodium selenite, 10-30ng/mL of epidermal growth factor, 0.1-0.5ng/mL of kinetin and 0.5-1mg/mL of glucan.
In a preferred embodiment of the present invention, in the first step, the main components comprise Human Serum Albumin (HSA) 4mg/mL, recombinant human insulin (rhuman insulin) 0.1mg/mL, fibronectin 10. mu.g/mL, L-glutamine 2mM, sodium selenite 0.2mg/mL, Epidermal Growth Factor (EGF) 10ng/mL, kinetin 0.1ng/mL, and dextran 0.5 mg/mL.
In a preferred embodiment of the present invention, the main components in the first step comprise 7mg/mL of human serum albumin, 0.5mg/mL of recombinant human insulin, 15. mu.g/mL of fibronectin, 7mM of L-glutamine, 0.5mg/mL of sodium selenite, 20ng/mL of epidermal growth factor, 0.3ng/mL of kinetin and 0.7mg/mL of dextran.
In a preferred embodiment of the present invention, in the first step, the main components comprise 10mg/mL of human serum albumin, 1mg/mL of recombinant human insulin, 20. mu.g/mL of fibronectin, 12mM of L-glutamine, 0.7mg/mL of sodium selenite, 30ng/mL of epidermal growth factor, 0.5ng/mL of kinetin and 1mg/mL of dextran.
As a preferred embodiment of the present invention, the mesenchymal cells include bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method can be applied to the preparation of cosmetics.
As a preferable scheme of the invention, the minimum effective concentration of the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method in cosmetics is 10-20 g/mL.
As a preferable scheme of the invention, the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method accounts for 15-35 wt% of the cosmetic.
The invention has the beneficial effects that: the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method of the serum protein complete culture solution of the mesenchymal cells can be applied to cosmetics, and nutrient components in the serum protein complete culture solution of the mesenchymal cells and cytokines generated by the mesenchymal cells in the proliferation growth process can be used for effectively supplementing nutrient repairing components to the skin cells, reducing the inflammation condition of the skin cells, slowing down the aging and apoptosis of the cells, and promoting the metabolism and proliferation growth of the cells, so that the damaged skin is repaired, the health state of the skin is recovered, the skin is smoother and more delicate, a new multi-effect cosmetic is provided for consumers, and the popularization prospect is good.
Detailed Description
Example 1
The preparation method of the serum protein complete culture solution of the mesenchymal cells comprises the following steps:
step one, selecting DMEM as a basic culture medium, and preparing main components according to the concentration proportion requirement, wherein the main components and the concentration are as follows:
step two, uniformly mixing the main components prepared in the step one, then selecting a filter membrane with the aperture of 0.1 mu m for filtration and sterilization to obtain a culture solution stock solution of the mesenchymal cells, and putting the culture solution stock solution into a culture dish;
step three, inoculating the mesenchymal cells into a culture dish, and culturing the mesenchymal cells;
and step four, when the cells are confluent and grow to 40%, filtering the culture solution in the culture dish, removing the mesenchymal cells, and obtaining the serum protein complete culture solution of the mesenchymal cells.
The mesenchymal cells comprise bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method can be applied to the preparation of cosmetics.
The minimum effective concentration of the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method in cosmetics is 10 g/mL.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method accounts for 15 wt% of the cosmetic.
Example 2
The preparation method of the serum protein complete culture solution of the mesenchymal cells comprises the following steps:
step one, selecting DMEM as a basic culture medium, and preparing main components according to the concentration proportion requirement, wherein the main components and the concentration are as follows:
step two, uniformly mixing the main components prepared in the step one, then selecting a filter membrane with the aperture of 0.3 mu m for filtration and sterilization to obtain a culture solution stock solution of the mesenchymal cells, and putting the culture solution stock solution into a culture dish;
step three, inoculating the mesenchymal cells into a culture dish, and culturing the mesenchymal cells;
and step four, when the cells are confluent and grow to 50%, filtering the culture solution in the culture dish, removing the mesenchymal cells, and obtaining the serum protein complete culture solution of the mesenchymal cells.
The mesenchymal cells comprise bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method can be applied to the preparation of cosmetics.
The minimum effective concentration of the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method in cosmetics is 15 g/mL.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method accounts for 25 wt% of the cosmetic.
Example 3
The preparation method of the serum protein complete culture solution of the mesenchymal cells comprises the following steps:
step one, selecting DMEM as a basic culture medium, and preparing main components according to the concentration proportion requirement, wherein the main components and the concentration are as follows:
step two, uniformly mixing the main components prepared in the step one, then selecting a filter membrane with the aperture of 0.5 mu m for filtration and sterilization to obtain a culture solution stock solution of the mesenchymal cells, and putting the culture solution stock solution into a culture dish;
step three, inoculating the mesenchymal cells into a culture dish, and culturing the mesenchymal cells;
and step four, when the cells are confluent and grow to 60%, filtering the culture solution in the culture dish, removing the mesenchymal cells, and obtaining the serum protein complete culture solution of the mesenchymal cells.
The mesenchymal cells comprise bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method can be applied to the preparation of cosmetics.
The minimum effective concentration of the serum protein complete culture solution of the mesenchymal cells prepared by the preparation method in cosmetics is 20 g/mL.
The serum protein complete culture solution of the mesenchymal cells prepared by the preparation method accounts for 35 wt% of the cosmetic.
Parts not described in detail herein are prior art.
Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (9)
1. A method for preparing a serum protein complete culture solution of mesenchymal cells is characterized by comprising the following steps:
selecting DMEM as a basic culture medium, and preparing main components according to concentration ratio requirements, wherein the main components comprise human serum albumin, recombinant human insulin, fibronectin, L-glutamine, sodium selenite, epidermal growth factors, kinetin and glucan;
step two, uniformly mixing the main components prepared in the step one, then selecting a filter membrane with the aperture of 0.1-0.5 mu m for filtration and sterilization to obtain a culture solution stock solution of the mesenchymal cells, and putting the culture solution stock solution into a culture dish;
step three, inoculating the mesenchymal cells into a culture dish, and culturing the mesenchymal cells;
and step four, filtering the culture solution in the culture dish when the cells grow to 40-60% in a confluent manner, and removing the mesenchymal cells to obtain the serum protein complete culture solution of the mesenchymal cells.
2. The method for preparing a serum protein complete culture solution of mesenchymal cells according to claim 1, wherein the method comprises the following steps: in the first step, the concentration ratio of the main components is as follows: 4-10mg/mL of human serum albumin, 0.1-1mg/mL of recombinant human insulin, 10-20 mu g/mL of fibronectin, 2-12mM of L-glutamine, 0.2-0.7mg/mL of sodium selenite, 10-30ng/mL of epidermal growth factor, 0.1-0.5ng/mL of kinetin and 0.5-1mg/mL of glucan.
3. The method for preparing a serum protein complete culture solution of mesenchymal cells according to claim 1, wherein the method comprises the following steps: in the first step, the main components comprise 4mg/mL of human serum albumin, 0.1mg/mL of recombinant human insulin, 10 mug/mL of fibronectin, 2mM of L-glutamine, 0.2mg/mL of sodium selenite, 10ng/mL of epidermal growth factor, 0.1ng/mL of kinetin and 0.5mg/mL of glucan.
4. The method for preparing a serum protein complete culture solution of mesenchymal cells according to claim 1, wherein the method comprises the following steps: in the first step, the main components comprise 7mg/mL of human serum albumin, 0.5mg/mL of recombinant human insulin, 15 mu g/mL of fibronectin, 7mM of L-glutamine, 0.5mg/mL of sodium selenite, 20ng/mL of epidermal growth factor, 0.3ng/mL of kinetin and 0.7mg/mL of glucan.
5. The method for preparing a serum protein complete culture solution of mesenchymal cells according to claim 1, wherein the method comprises the following steps: in the first step, the main components comprise 10mg/mL of human serum albumin, 1mg/mL of recombinant human insulin, 20 mu g/mL of fibronectin, 12mM of L-glutamine, 0.7mg/mL of sodium selenite, 30ng/mL of epidermal growth factor, 0.5ng/mL of kinetin and 1mg/mL of glucan.
6. The method for preparing a serum protein complete culture solution of mesenchymal cells according to claim 1, wherein the method comprises the following steps: the mesenchymal cells comprise bone marrow mesenchymal stem cells and umbilical cord mesenchymal stem cells.
7. Use of a complete serum protein culture of mesenchymal cells obtained by the method according to any one of claims 1 to 6 in cosmetics.
8. Use according to claim 7, characterized in that: the minimum effective concentration of the serum protein complete culture solution of the mesenchymal cells in the cosmetics is 10-20 g/mL.
9. Use according to claim 7, characterized in that: the serum protein complete culture solution of the mesenchymal cells accounts for 15-35 wt% of the cosmetic.
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