CN113637626A - Method for improving survival rate of saccharomyces rouxii under high-salt stress condition - Google Patents
Method for improving survival rate of saccharomyces rouxii under high-salt stress condition Download PDFInfo
- Publication number
- CN113637626A CN113637626A CN202111136706.7A CN202111136706A CN113637626A CN 113637626 A CN113637626 A CN 113637626A CN 202111136706 A CN202111136706 A CN 202111136706A CN 113637626 A CN113637626 A CN 113637626A
- Authority
- CN
- China
- Prior art keywords
- salt stress
- saccharomyces rouxii
- salt
- survival rate
- under high
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a method for improving the survival rate of Saccharomyces rouxii under high salt stress conditions. The invention solves the technical problem of providing a method for improving the survival rate of the saccharomyces rouxii under the condition of high salt stress, which comprises the following steps: (1) pre-adapting or heat pre-adapting the salt of the saccharomyces rouxii seed liquid; (2) and culturing the pre-adapted saccharomyces rouxii seed solution under the condition of high salt stress. According to the invention, the survival rate of the saccharomyces rouxii under the high salt stress condition can be improved after the saccharomyces rouxii seed liquid is subjected to salt pre-adaptation treatment or heat pre-adaptation treatment. Compared with the saccharomyces rouxii which is not subjected to salt pretreatment or heat pretreatment, the survival rate of the saccharomyces rouxii subjected to the salt pretreatment or heat pretreatment can be respectively improved by 2.52 times and 2.23 times under the condition of high salt stress.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a method for improving the survival rate of Saccharomyces rouxii under high salt stress conditions.
Background
Saccharomyces rouxii (Zygosaccharomyces rouxii) has important functions of producing alcohol, aroma, freshness and the like, and is widely applied to the production process of traditional fermented foods such as bean paste, soy sauce, miso and the like. However, these fermented foods are often produced under high osmotic pressure conditions, and the Saccharomyces rouxii inevitably suffers from salt stress, acid stress, oxygen stress, and the like, and particularly, salt stress, which is a major stress factor, affects the growth and physiological activity of cells, thereby deteriorating the quality of the products. Therefore, the method has important practical value for improving the growth performance of the saccharomyces rouxii under the condition of high salt stress.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a method for improving the survival rate of the saccharomyces rouxii under the condition of high salt stress, which comprises the following steps:
(1) pre-adapting or heat pre-adapting the salt of the saccharomyces rouxii seed liquid;
(2) and culturing the pre-adapted saccharomyces rouxii seed solution under the condition of high salt stress.
Further, the salt pre-adaptation treatment is to inoculate the saccharomyces rouxii seed solution into the salt pre-adaptation culture solution.
Further, the salt pre-adaptation culture solution contains 110-130g/L NaCl.
Preferably, the salt preconditioning broth comprises; 8-12g/L of yeast extract, 15-25g/L of peptone, 15-25g/L of glucose and 130g/L of NaCl 110-.
Further, the pH of the salt pre-adaptation culture solution is 5.8-6.2.
Further, the salt pre-conditioning temperature is 28-32 ℃.
Preferably, the salt pre-conditioning temperature is 30 ℃.
Further, the salt pretreatment time is 80-100 min.
Further, the salt pre-adaptation treatment is to centrifuge the cultured saccharomyces rouxii seed liquid at 7000-10000r/min at 3-5 ℃ for 5-10min to collect thalli, suspend the thalli in the salt pre-adaptation culture liquid, and carry out salt pre-adaptation treatment at 30 ℃ for 80-100 min.
Further, the thermal pre-conditioning treatment temperature is 35-40 ℃.
Further, the thermal pre-adaptation treatment time is 80-100 min.
Further, the thermal pre-adaptation treatment is to centrifuge the cultured seed solution, collect thalli, suspend the thalli in a fresh seed culture solution, and perform thermal pre-adaptation treatment for 80-100min at 35-40 ℃.
Preferably, the thermal pre-adaptation treatment is to centrifuge the cultured seed solution at 7000-10000r/min at 3-5 ℃ for 5-10min to collect the thallus, suspend the thallus in the fresh seed culture solution, and perform thermal pre-adaptation treatment at 35-40 ℃ for 80-100 min.
Preferably, the fresh seed culture fluid comprises: 8-12g/L yeast extract, 15-25g/L peptone and 15-25g/L glucose; the pH value is 5.8-6.2.
Wherein, in the step (2), the NaCl concentration of the high-salt stress culture solution is 300-350 g/L.
Preferably, the high-salt stress culture solution comprises 8-12g/L yeast extract, 15-25g/L peptone, 15-25g/L glucose and 300-350g/L NaCl.
Further, the pH of the high-salt stress culture solution is 5.8-6.2.
Further, the culture temperature of high salt stress is 28-32 ℃.
Further, the high salt stress culture time is 80-100 min.
Further, the culture under the high-salt stress condition is to take the seed solution which is subjected to pre-adaptation treatment, centrifuge at 3-5 ℃ for 5-10min at 7000-10000r/min to collect thalli, suspend the thalli in the high-salt stress culture solution, and culture the thalli at 28-32 ℃ for 80-100min under the high-salt stress condition.
The method for improving the survival rate of the saccharomyces rouxii under the high-salt stress condition further comprises a survival rate measuring method.
Wherein the determination method comprises: centrifuging the culture solution subjected to high-salt stress treatment at 7000-10000r/min at 3-5 ℃ for 5-10min, collecting thalli, suspending the thalli in a fresh seed culture medium, coating 10 mu L of thalli in a solid plate culture medium, performing static culture at 28-32 ℃ for 72h, and calculating the number of viable bacteria.
Further, the solid plate medium includes: 8-12g/L yeast extract, 15-25g/L peptone, 15-25g/L glucose and 18-22g/L agar.
Further, the solid plate medium has a pH of 5.8 to 6.2.
Wherein the preservation number of the Saccharomyces rouxii (Zygosaccharomyces rouxii) is CGMCC No. 3791. The preservation time is 4 months and 29 days in 2010, and the preservation place is China general microbiological culture Collection center; the address is No.3 Xilu Beijing province of Chaoyang, the institute of microbiology, China academy of sciences, zip code: 100101.
the invention has the beneficial effects that:
according to the invention, the survival rate of the saccharomyces rouxii under the high salt stress condition can be improved after the saccharomyces rouxii seed liquid is subjected to 110-sodium chloride (NaCl) concentration salt pre-adaptation treatment or 35-40 ℃ heat pre-adaptation treatment. Compared with the saccharomyces rouxii which is not subjected to salt pretreatment or heat pretreatment, the survival rate of the saccharomyces rouxii subjected to the salt pretreatment or heat pretreatment can be respectively improved by 2.52 times and 2.23 times under the condition of high salt stress.
Drawings
FIG. 1 shows the results of counting the number of colonies of examples 1 and 2 and comparative examples 1 to 3.
Detailed Description
In order to improve the growth performance of the saccharomyces rouxii under the high-salt stress condition, the invention provides a method for improving the survival rate of the saccharomyces rouxii under the high-salt stress condition, which comprises the following steps:
(1) inoculating the glycerol tube stock solution of the saccharomyces rouxii preserved at the temperature of minus 80 ℃ into a seed culture medium in an inoculation amount of 5% (V/V), and performing static culture at the temperature of 30 ℃ for 24 hours to logarithmic phase to obtain a seed culture solution.
(2) Pre-adapting or heat pre-adapting the salt of the saccharomyces rouxii seed liquid.
Because salt and heat are environmental conditions frequently encountered in the fermentation of the saccharomyces rouxii, heat is generated in the fermentation process, and the pickling fermentation process is a high-salt environment, the method performs salt pre-adaptation or heat pre-adaptation treatment on the saccharomyces rouxii seed liquid, and is more favorable for the survival of the saccharomyces rouxii under the conditions.
After the applicant searches for the salt concentration in the early stage, the NaCl in the salt pre-adaptation culture solution is controlled to be 130g/L at 110-. If the NaCl in the salt pre-adaptation culture solution is not controlled to be 110-130g/L and the culture condition of the salt pre-adaptation culture solution is not controlled to be within the above range, the survival rate improving effect is not obvious.
The salt pretreatment of the invention is to centrifuge the cultured saccharomyces rouxii seed liquid at 7000-10000r/min at 3-5 ℃ for 5-10min to collect thalli, suspend the thalli in the salt pretreatment culture liquid, and carry out the salt pretreatment at 30 ℃ for 80-100 min.
Since the growth temperature of the microorganism is 28-32 ℃ and the optimum temperature for the growth of the Saccharomyces rouxii is 30 ℃, the pre-adaptation culture temperature of the salt of the invention is 28-32 ℃, preferably 30 ℃.
The thermal pre-adaptation treatment of the invention is that the cultured seed liquid is centrifuged for 5-10min at 3-5 ℃ at 7000-10000r/min to collect thalli, and the thalli is suspended in yeast extract of 8-12g/L, peptone of 15-25g/L and glucose of 15-25 g/L; performing thermal pre-adaptation treatment at 35-40 deg.C for 80-100min in fresh seed culture solution with pH of 5.8-6.2.
Through a large amount of research and exploration, the applicant finds that the temperature of the thermal pre-adaptation treatment is controlled to be 35-40 ℃, the treatment time is controlled to be 80-100min, and the survival rate of the saccharomyces rouxii subjected to high salt stress is improved most beneficially.
(3) Taking the seed liquid after the pre-adaptation treatment, centrifuging at 3-5 ℃ for 5-10min at 7000 plus 10000r/min to collect thalli, suspending the thalli in a high-salt stress culture solution containing 8-12g/L yeast extract, 15-25g/L peptone, 15-25g/L glucose, 300 plus 350g/L NaCl and pH of 5.8-6.2, and culturing at 28-32 ℃ for 80-100min under high-salt stress.
The temperature, time and treatment mode of salt pre-adaptation, heat pre-adaptation treatment and high-salt stress culture are conditions with optimal effect obtained by exploring and optimizing the early conditions. Exceeding the above-mentioned conditions, the survival rate promotion effect is not obvious. Meanwhile, the microorganism is used as a living organism, the temperature treatment is not too high or too low, the time is not too long, and the strain directly dies. From the industrial production point of view, the treatment time is too long, the energy consumption is increased, and the production benefit is reduced, so the time is generally fixed within a few hours.
The method for improving the survival rate of the saccharomyces rouxii under the high-salt stress condition further comprises a survival rate measuring method.
The specific determination method comprises the following steps: centrifuging the culture solution subjected to high-salt stress treatment at 7000-10000r/min at 3-5 ℃ for 5-10min, collecting thalli, suspending the thalli in a fresh seed culture medium, coating 10 mu L of thalli in a solid plate culture medium, performing static culture at 28-32 ℃ for 72h, and calculating the number of viable bacteria.
Wherein the solid plate medium comprises: 8-12g/L yeast extract, 15-25g/L peptone, 15-25g/L glucose and 18-22g/L agar.
Wherein the pH of the solid plate culture medium is 5.8-6.2.
The preservation number of the saccharomyces rouxii (Zygosaccharomyces rouxii) is CGMCC No. 3791. The preservation time is 4 months and 29 days in 2010, and the preservation place is China general microbiological culture Collection center; the address is No.3 Xilu Beijing province of Chaoyang, the institute of microbiology, China academy of sciences, zip code: 100101.
the embodiments of the present invention will be explained below with reference to specific examples and comparative examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1
(1) Inoculating a strain preserved in glycerol storage liquid at the temperature of minus 80 ℃, namely Saccharomyces rouxii (Zygosaccharomyces rouxii) CGMCC No.3791 in a seed culture medium according to the inoculation amount of 5% (V/V), and performing standing culture at the temperature of 30 ℃ for 24h to a logarithmic phase to obtain a seed culture liquid.
(2) Salt pretreatment: inoculating the seed culture solution into a salt pre-adaptation culture solution with NaCl concentration of 120g/L for 90min by using an inoculation amount with a volume ratio of 5% (V/V).
(3) Centrifuging the culture solution after salt treatment for 5min at 8000r/min (4 ℃), collecting thallus, suspending the thallus in a high-salt stress culture medium with NaCl concentration of 350g/L, and culturing for 90min at 30 ℃ under high-salt stress.
(4) Centrifuging the culture solution after high salt stress treatment for 5min at 8000r/min (4 ℃), collecting thallus, suspending thallus in fresh seed culture medium, spreading 10 μ L of thallus in solid plate culture medium, standing and culturing at 30 ℃ for 72h, and calculating colony count, wherein the result is shown in FIG. 1.
Example 2
(1) Inoculating a strain preserved in glycerol storage liquid at-80 ℃ and Saccharomyces rouxii (Zygosaccharomyces rouxii) CGMCC No.3791 in a seed culture medium in an inoculation amount of 5%, and performing static culture at 30 ℃ for 24h to logarithmic phase to obtain a seed culture liquid.
(2) Thermal pre-adaptation treatment: inoculating the seed culture solution into fresh seed culture solution at an inoculation amount of 5% (V/V) by volume, and performing thermal pre-adaptation treatment at 37 ℃ for 90 min.
(3) Centrifuging the heat-treated culture solution at 8000r/min (4 deg.C) for 5min to collect thallus, suspending thallus in high salt stress culture medium with NaCl concentration of 350g/L, and culturing at 30 deg.C for 90min under high salt stress.
(4) Centrifuging the culture solution after high salt stress treatment for 5min at 8000r/min (4 deg.C), collecting thallus, suspending thallus in fresh seed culture medium, spreading 10 μ L of thallus in solid plate culture medium, standing at 30 deg.C for 72h, and calculating viable count, the result is shown in FIG. 1.
Comparative example 1
(1) Inoculating a strain preserved in glycerol storage liquid at the temperature of minus 80 ℃, namely Saccharomyces rouxii (Zygosaccharomyces rouxii) CGMCC No.3791 in a seed culture medium according to the inoculation amount of 5% (V/V), and performing standing culture at the temperature of 30 ℃ for 24h to a logarithmic phase to obtain a seed culture liquid.
(2) Acid pretreatment: inoculating the seed culture solution into a second culture solution with pH of 4.5 at an inoculation amount of 5% (V/V) by volume ratio for acid pretreatment for 90 min.
(3) Centrifuging the culture solution after acid treatment for 5min at 8000r/min (4 ℃), collecting thallus, suspending the thallus in a high-salt stress culture medium with NaCl concentration of 350g/L, and culturing for 90min at 30 ℃ under high-salt stress.
(4) Centrifuging the culture solution after high salt stress treatment for 5min at 8000r/min (4 ℃), collecting thallus, suspending thallus in fresh seed culture medium, spreading 10 μ L of thallus in solid plate culture medium, standing and culturing at 30 ℃ for 72h, and calculating colony count, wherein the result is shown in FIG. 1.
Comparative example 2
(1) Inoculating a strain preserved in glycerol storage liquid at the temperature of minus 80 ℃, namely Saccharomyces rouxii (Zygosaccharomyces rouxii) CGMCC No.3791 in a seed culture medium according to the inoculation amount of 5% (V/V), and performing standing culture at the temperature of 30 ℃ for 24h to a logarithmic phase to obtain a seed culture liquid.
(2) Oxygen pretreatment: inoculating the seed culture solution into a third culture solution with H2O2 concentration of 0.034g/L for 90min at an inoculation amount of 5% (V/V).
(3) Centrifuging the oxygen-treated culture solution at 8000r/min (4 deg.C) for 5min to collect thallus, suspending thallus in high salt stress culture medium with NaCl concentration of 350g/L, and culturing at 30 deg.C for 90min under high salt stress.
(4) Centrifuging the culture solution after high salt stress treatment for 5min at 8000r/min (4 ℃), collecting thallus, suspending thallus in fresh seed culture medium, spreading 10 μ L of thallus in solid plate culture medium, standing and culturing at 30 ℃ for 72h, and calculating colony count, wherein the result is shown in FIG. 1.
Comparative example 3
This comparative example 3 provides a culture method of saccharomyces rouxii under high salt stress conditions, which is substantially the same as the procedure of example 2, except that in the step (2) heat pre-adaptation treatment, this comparative example 3 is a normal treatment at 30 ℃ for 90 min. The results are shown in FIG. 1.
In conclusion, the survival rate of the saccharomyces rouxii subjected to the salt pre-adaptation or heat pre-adaptation treatment is obviously higher than that of the saccharomyces rouxii subjected to the acid pre-adaptation or oxygen pre-adaptation treatment under the high-salt stress condition. And the treatment time and the treatment temperature have great influence on the survival rate of the saccharomyces rouxii.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention. It will be apparent to those skilled in the art that the same may be substituted or modified for the above embodiments in accordance with the present invention, and all such equivalent changes and modifications are intended to be included within the scope of the present invention.
Claims (10)
1. The method for improving the survival rate of the saccharomyces rouxii under the high-salt stress condition is characterized by comprising the following steps: the method comprises the following steps:
(1) pre-adapting or heat pre-adapting the salt of the saccharomyces rouxii seed liquid;
(2) and culturing the pre-adapted saccharomyces rouxii seed solution under the condition of high salt stress.
2. The method of increasing survival rate of saccharomyces rouxii under high salt stress conditions as claimed in claim 1, wherein: the salt pretreatment is to inoculate the saccharomyces rouxii seed solution into a salt pretreatment culture solution; the culture solution contains 110-130g/L NaCl; preferably, the salt preconditioning broth comprises; 8-12g/L of yeast extract, 15-25g/L of peptone, 15-25g/L of glucose and 130g/L of NaCl 110-; the pH of the salt pre-adaptation culture solution is 5.8-6.2.
3. The method for improving survival rate of saccharomyces rouxii under high salt stress conditions according to claim 1 or 2, wherein: the salt pre-conditioning temperature is 28-32 ℃.
4. The method for improving survival rate of Saccharomyces rouxii under high salt stress conditions according to any one of claims 1 to 3, wherein: the salt pre-conditioning time is 80-100 min.
5. The method of increasing survival rate of saccharomyces rouxii under high salt stress conditions as claimed in claim 1, wherein: the thermal pre-adaptation treatment temperature is 35-40 ℃.
6. The method of increasing survival rate of Saccharomyces rouxii under high salt stress conditions as claimed in claim 5, wherein: the thermal pre-adaptation treatment time is 80-100 min.
7. The method for improving survival rate of Saccharomyces rouxii under high salt stress conditions according to claim 5 or 6, wherein: the thermal pre-adaptation treatment is to centrifuge the cultured seed solution, collect the thalli, then suspend the thalli in the fresh seed culture solution, and perform thermal pre-adaptation treatment for 80-100min at 35-40 ℃.
8. The method of increasing survival rate of saccharomyces rouxii under high salt stress conditions as claimed in claim 1, wherein: the NaCl concentration of the high-salt stress culture solution is 300-350 g/L; preferably, the high-salt stress culture solution comprises 8-12g/L yeast extract, 15-25g/L peptone, 15-25g/L glucose, and 300-350g/L NaCl; the pH value of the high-salt stress culture solution is 5.8-6.2.
9. The method of increasing survival rate of saccharomyces rouxii under high salt stress conditions as claimed in claim 8, wherein: the high-salt stress culture temperature is 28-32 deg.C, and the high-salt stress culture time is 80-100 min.
10. The method for improving survival rate of Saccharomyces rouxii under high salt stress conditions according to any one of claims 1 to 9, wherein: the preservation number of the saccharomyces rouxii is CGMCC No. 3791.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136706.7A CN113637626B (en) | 2021-09-27 | 2021-09-27 | Method for improving survival rate of Russell yeast under high-salt stress condition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136706.7A CN113637626B (en) | 2021-09-27 | 2021-09-27 | Method for improving survival rate of Russell yeast under high-salt stress condition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113637626A true CN113637626A (en) | 2021-11-12 |
| CN113637626B CN113637626B (en) | 2023-07-11 |
Family
ID=78426249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111136706.7A Active CN113637626B (en) | 2021-09-27 | 2021-09-27 | Method for improving survival rate of Russell yeast under high-salt stress condition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113637626B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350725A (en) * | 2022-01-14 | 2022-04-15 | 四川大学 | Saccharomycete extracellular polysaccharide, preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838658A (en) * | 2015-12-02 | 2016-08-10 | 四川大学 | Method for improving biomass of lactic acid bacteria under high salt condition |
| CN105838636A (en) * | 2015-12-02 | 2016-08-10 | 四川大学 | Method for improving biomass of lactic acid bacteria under high salt condition |
| CN110283771A (en) * | 2019-07-24 | 2019-09-27 | 四川大学 | The method for improving survival rate of the methamidophos under stress conditions |
| CN111500476A (en) * | 2020-05-20 | 2020-08-07 | 四川大学 | Method for improving alcohol tolerance of saccharomycetes by utilizing lactic acid bacteria |
-
2021
- 2021-09-27 CN CN202111136706.7A patent/CN113637626B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838658A (en) * | 2015-12-02 | 2016-08-10 | 四川大学 | Method for improving biomass of lactic acid bacteria under high salt condition |
| CN105838636A (en) * | 2015-12-02 | 2016-08-10 | 四川大学 | Method for improving biomass of lactic acid bacteria under high salt condition |
| CN110283771A (en) * | 2019-07-24 | 2019-09-27 | 四川大学 | The method for improving survival rate of the methamidophos under stress conditions |
| CN111500476A (en) * | 2020-05-20 | 2020-08-07 | 四川大学 | Method for improving alcohol tolerance of saccharomycetes by utilizing lactic acid bacteria |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350725A (en) * | 2022-01-14 | 2022-04-15 | 四川大学 | Saccharomycete extracellular polysaccharide, preparation method and application thereof |
| CN114350725B (en) * | 2022-01-14 | 2023-05-05 | 四川大学 | Yeast extracellular polysaccharide, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113637626B (en) | 2023-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11242502B2 (en) | Low urea-producing and flavor-producing Wickerhamomyces anomalus strain and use thereof in food production | |
| CN109370929B (en) | Application of saccharomyces cerevisiae in brewing wine | |
| CN110283771B (en) | Method for improving survival rate of saccharomyces rouxii under stress condition | |
| US10822625B2 (en) | Method for domesticating Saccharomyces cerevisiae | |
| CN106754508B (en) | Bacillus subtilis strain and application thereof in soy sauce fermentation and aroma enhancement | |
| CN114107077B (en) | Ester-producing yeast strain and application thereof | |
| CN110760452B (en) | Lu's combined yeast and application thereof in soybean paste fermentation | |
| CN109971663B (en) | Thermotolerant yarrowia lipolytica yeast and application thereof | |
| CN109971661B (en) | Ester-producing yeast ZB406 and application thereof | |
| CN103451133A (en) | Bacillus circulans and application for same in preparation for ferulic acid decarboxylase | |
| CN111662860A (en) | Method for improving survival rate of tetragenococcus halophilus under extreme conditions | |
| CN112852664A (en) | Saccharomyces cerevisiae and method for improving yield of gamma-aminobutyric acid produced by saccharomyces cerevisiae | |
| CN113637626A (en) | Method for improving survival rate of saccharomyces rouxii under high-salt stress condition | |
| CN111534453B (en) | Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application thereof | |
| CN116496969A (en) | Method for improving lactic acid tolerance by exogenously adding arginine | |
| CN114250159B (en) | Two wild yeasts and application thereof in improving color stability of fruit wine | |
| CN114561328A (en) | Preparation method of high-activity lactobacillus agent | |
| CN108949595B (en) | Aroma-producing yeast and application thereof in red yeast rice yellow wine brewing | |
| CN106916875B (en) | Culture medium for identifying acetobacter and gluconobacter | |
| CN113293106B (en) | Fungus of genus Filobasidium of class Ascomycetes and application thereof | |
| CN108587923B (en) | Method for improving malic acid fermentation performance | |
| CN112175847A (en) | Novel strain of Plectosporium and application thereof | |
| CN113151014A (en) | Simple and rapid monascus separating and storing method | |
| CN111411135A (en) | Fermentation production process of purine | |
| CN115322910B (en) | Russell yeast and mutagenesis screening method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |