CN113607717A - Chemiluminescence detection method, device and system - Google Patents
Chemiluminescence detection method, device and system Download PDFInfo
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Abstract
The invention discloses a chemiluminescence detection method, which is used for detecting an optical signal emitted by a reaction area to be detected and comprises the following steps: and acquiring the optical signal emitted by the reaction area by using a silicon-based photomultiplier. On the basis of the above, the invention also discloses a chemiluminescence detection device and system. The detection method, the device and the system can solve the problem of considering both the detection cost and the detection sensitivity. In addition, in the detection system provided by the invention, the microfluidic chip is combined with the chemiluminescence detection device, so that the problems of detection cost and detection sensitivity can be solved, and the volume of the detection system can be further reduced.
Description
Technical Field
The present invention relates to the field of chemiluminescence detection. More particularly, the present invention relates to methods, devices and systems for chemiluminescence detection.
Background
Chemiluminescence is a phenomenon in which photons are generated by chemical changes of chemical substances under specific conditions. The detection chip is used for detecting photons generated by chemiluminescence, and can be combined with high-specificity immunoreaction to be used for detecting various antigens, haptens, antibodies, small molecules, proteins and the like. With the development of scientific technology and medical level, various chemiluminescence immunoassay analyzers which are based on chemiluminescence principles, are used for auxiliary diagnosis of diseases and can accurately detect markers of various diseases are produced.
In order to obtain the photon condition generated by chemiluminescence, a photomultiplier tube (PMT for short), a charge-coupled device image sensor (CCD for short), or a Complementary Metal Oxide Semiconductor (CMOS) chip is generally used for detection. Unfortunately, the detection methods using the above chip structures have many defects in some aspects, and it is difficult to combine the detection cost and the detection sensitivity.
First, although the detection method constructed by the PMT chip can well meet the requirement of detection sensitivity, the chemiluminescent analyzer using the PMT chip is expensive and limited in application due to high operating voltage, high integration difficulty and high manufacturing cost required by the PMT, and particularly, the product price disadvantage using the PMT chip is more obvious in the application of primary medical institutions.
Moreover, the defects of the CCD or CMOS are more prominent than those of the PMT. On the one hand, the sensitivity of CCD or CMOS is relatively low due to lack of photoelectric internal gain, and it is difficult to satisfy the requirement of chemiluminescence detection for high sensitivity, so that it can be mainly used only in products for fluorescence detection. On the other hand, like PMT, CCD or CMOS is also high in cost and is difficult to be widely used.
Therefore, in the field of chemiluminescence detection, how to construct a new chemiluminescence detection method on the basis of considering both detection cost and detection sensitivity is a very difficult problem to solve.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
Another object of the present invention is to provide a method, an apparatus and a system for detecting chemiluminescence, which can solve the problem of both detection cost and detection sensitivity. In addition, in the chemiluminescence detection system provided by the invention, the microfluidic chip and the chemiluminescence detection device are combined together, so that the problems of detection cost and detection sensitivity can be solved, and the volume of the detection system can be further reduced.
Specifically, the invention is realized by the following technical scheme:
first aspect of the invention
In a first aspect, there is provided a chemiluminescent detection method for detecting an optical signal emitted from a reaction area to be detected, the detection method comprising:
and acquiring a light signal emitted by the reaction area to be detected by using a silicon-based photomultiplier.
In some embodiments, the detection method further comprises:
converting the optical signal into an electrical signal through the silicon-based photomultiplier;
amplifying the electrical signal on the silicon-based photomultiplier tube to generate an amplified electrical signal;
in some embodiments, the detection method further comprises: and calculating the photon number corresponding to the optical signal according to the amplified electric signal.
Second aspect of the invention
A second aspect provides a chemiluminescent photodetecting device for detecting an optical signal emitted from a reaction region to be detected, the photodetecting device comprising a silicon-based photomultiplier and a control unit, wherein,
the silicon-based photomultiplier is used for performing the following work: acquiring an optical signal emitted by the reaction area; converting the optical signal into an electrical signal; and amplifying the electrical signal to generate an amplified electrical signal;
the control unit is used for controlling the silicon-based photomultiplier to work and sending the amplified electric signal to a counting unit.
In some embodiments, the photodetecting device further comprises a heat dissipation mechanism, and the heat dissipation mechanism comprises at least one of a refrigerator and a heat sink.
In some embodiments, when the heat dissipation mechanism includes a heat dissipation plate, a heat dissipation channel is disposed in the heat dissipation plate, and the silicon-based photomultiplier is disposed in the heat dissipation channel.
Third aspect of the invention
A third aspect provides a chemiluminescent photodetecting system comprising:
the microfluidic chip comprises a reaction tank, wherein the reaction tank is used for providing a reaction area to be detected;
a photodetecting device according to the second aspect.
In some embodiments, the chemiluminescent reaction occurring within the reaction cell of the microfluidic chip is a homogeneous chemical reaction.
In some technical schemes, a chemiluminescence reaction generated in a reaction tank of the microfluidic chip is a semi-homogeneous phase chemical reaction, and the microfluidic chip is provided with magnetic beads and a magnetic bead channel for placing the magnetic beads; the photoelectric detection system also comprises a magnet module, and the magnet module is used for collecting and dragging the magnetic beads in the magnetic bead channel.
In some technical schemes, the chemiluminescence reaction occurring in the reaction cell of the microfluidic chip is a heterogeneous chemical reaction, and the microfluidic chip is directly or indirectly coated with protein or nucleic acid specifically bound with the substance to be detected in the analysis sample;
the photoelectric detection system also comprises an air pump, and the air pump is used for providing power required by the movement of liquid in the micro-channel of the micro-fluidic chip.
In some embodiments, the photodetection system further comprises a temperature control device for adjusting the temperature of the reaction cell. The temperature control means can be realized by means of the prior art.
In some aspects, the microfluidic chip comprises a carrier member; the carrying component is used for adding an object to be detected, a substrate or an excitation liquid into the reaction tank; it should be noted that, in some technical schemes, the substance to be detected, the substrate or the excitation liquid may also be added into the reaction tank through other technical schemes, and specifically, the setting may be performed by those skilled in the art according to actual needs;
the photoelectric detection system also comprises a driving motor, and the driving motor is used for driving the carrying component to move so as to add the object to be detected and the substrate or the excitation liquid into the reaction tank.
In some technical schemes, the carrying component comprises a channel for accommodating an object to be detected, a substrate or an excitation liquid, the microfluidic chip further comprises a cavity channel and a circulation path, the cavity channel is communicated with the reaction tank through the circulation path, and the carrying component is connected to the inside of the cavity channel in a sliding manner;
when the carrying component slides to a preset position in the cavity channel, the channel is communicated with the flowing path, so that the object to be detected, the substrate or the exciting liquid loaded or contained in the channel can flow into the reaction pool.
In some technical solutions, the photodetection system further includes a portable terminal, and the portable terminal is in communication connection with the driving motor and is configured to control the driving motor to operate. The portable terminal may be a general-purpose computer such as a notebook computer, a tablet computer, a smart phone, and the like.
In some aspects, the portable terminal includes a memory, a processor, and at least one application stored in the memory and configured to be executed by the processor, the at least one application configured to perform the steps of: and receiving the amplified electric signal sent by the photoelectric detection device and counting.
The technical effect of the technical scheme of the invention at least comprises the following steps:
in some embodiments, the method of the present application receives an optical signal emitted from a reaction region through a photosensitive film on a silicon-based photomultiplier (SiPM). Compared with the prior art, the method has the following beneficial effects: the photon detection module constructed by the silicon-based photomultiplier can obtain high gain under the low working voltage of only 25-30V, and SiPM can accurately obtain an optical signal emitted by a reaction area to be detected without a booster circuit; in addition, in a low-voltage working environment, the safety of the SiPM circuit can be improved, and the risk of circuit failure is reduced. In addition, the peripheral circuit of the SiPM is simple, so that the usability of the SiPM in acquiring the optical signal emitted by the reaction area to be detected is improved. In addition, the SiPM has a very compact and light structure, the volume is far smaller than that of the PMT, the production cost is far lower than that of the PMT, and the cost of a chemiluminescence detection instrument can be greatly reduced.
In some technical schemes, the chemiluminescence detection system provided by the invention combines a microfluidic chip with a chemiluminescence detection device, so that the problems of detection cost and detection sensitivity can be solved, and the volume of the detection system can be further reduced.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic flow diagram of a chemiluminescent detection method of the present invention in some embodiments;
FIG. 2 is a schematic view of a chemiluminescent detection device of the present invention in some embodiments;
FIG. 3 is a schematic view of a fin member of the present invention in some embodiments;
FIG. 4 is a schematic view of the connection of the heat sink member of the present invention to a silicon-based photomultiplier tube in some embodiments;
FIG. 5 is a schematic view of a chemiluminescent detection system of the present invention in some embodiments;
FIG. 6 is a schematic view of a drive motor, microfluidic chip and temperature control device of the present invention in some embodiments;
fig. 7 is a schematic diagram of a microfluidic chip of the present invention in some embodiments;
FIG. 8 is a schematic view of a chemiluminescent detection system of the present invention in further embodiments;
reference numerals: 1. a photoelectric detection system; 10. a photoelectric detection device; 110. a silicon-based photomultiplier tube; 120. a control unit; 130. a heat dissipation mechanism; 131. a heat sink member; 1311. a fin element; 1312. A heat dissipation channel; 1313. a base; 1314. sealing the baffle plate; 20. a microfluidic chip; 210. a carrier member; 211. a channel; 220. a reaction tank; 230. a lumen; 240. a flow path; 30. a drive motor; 40. a temperature control device; 50. a portable terminal.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
The terms "first", "second" and "third" in the embodiments of the present application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of the feature. It is also noted that, in the present application, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise. Further, the orientations and positional relationships indicated by the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like are based on the orientations and positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or apparatus referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "include" and "provided," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
In addition to the foregoing, it should be emphasized that reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
It should be noted that the term "Homogeneous chemical reaction" herein refers to a Homogeneous reaction (also called "single-phase reaction"), i.e. a chemical reaction that occurs only in one phase (gas, liquid or solid). Characterized in that no phase interface is present in the reaction system. For example, the high-temperature thermal cracking of hydrocarbons to produce ethylene is an important gas-phase homogeneous reaction, and the acid-base neutralization, esterification, saponification and other reactions are typical liquid-phase homogeneous reactions.
The term "Heterogeneous chemical reaction" refers to a Heterogeneous reaction (also called "Heterogeneous reaction"), in which reactants are components of two or more phases (solid and gas, solid and liquid, two immiscible liquids), or a general term for chemical reactions in which one or more reactants are carried out at an interface, such as on the surface of a solid catalyst. In the heterogeneous catalysis process in the environment, for example, when sulfur dioxide in the atmosphere contacts with metal ions such as manganese, iron and the like contained on the surface of particulate matters, catalytic oxidation can be carried out to generate sulfate; the oxidation and corrosion of sulfur dioxide on metal materials and buildings are heterogeneous reactions.
The term "semi-phase chemical reaction" refers to a chemical reaction between a "homogeneous chemical reaction" and a "heterogeneous chemical reaction".
First aspect of the invention
In a first aspect, there is provided a method of detecting chemiluminescence, comprising:
s101, a silicon-based photomultiplier 110 is used for acquiring an optical signal emitted by a reaction area to be detected.
In the method of the present application, the optical signal from the reaction region is received by a photosensitive film on the silicon-based photomultiplier tube 110. Compared with the prior art, the chemiluminescence detection method of the first aspect has at least the following beneficial effects: when the traditional PMT technology is adopted for optical signal detection, the PMT needs high voltage of about 1kV to 2 kV; under such high voltage operating conditions, the product needs to be prevented from being broken down, so that a supporting circuit and a protection component with a complex process are needed in the using process, and the cost is high. In the application, the photon detection module constructed by the silicon-based photomultiplier 110 can obtain high gain under the low working voltage of only 25-30V, and the SiPM can accurately obtain an optical signal emitted by a reaction area to be detected without a booster circuit; in addition, in a low-voltage working environment, the safety of the SiPM circuit can be improved, and the risk of circuit failure is reduced. In addition, the peripheral circuit of the SiPM is simple, so that the usability of the SiPM in acquiring the optical signal emitted by the reaction area to be detected is improved; in addition, the SiPM has a very compact and light structure, the volume is far smaller than that of the PMT, the production cost is far lower than that of the PMT, and the cost for manufacturing a chemiluminescence detection instrument can be greatly reduced.
In some embodiments, the detection method further comprises:
s102, converting the optical signal into an electric signal through the silicon-based photomultiplier 110;
and S103, amplifying the electric signal on the silicon-based photomultiplier 110 to generate an amplified electric signal. (ii) a
In some embodiments, the detection method further comprises:
and S104, calculating the photon number corresponding to the optical signal according to the amplified electric signal.
Second aspect of the invention
As shown in fig. 2, the second aspect provides a chemiluminescent photodetecting device 10 for detecting an optical signal emitted from a reaction region to be detected, the photodetecting device 10 comprising a silicon-based photomultiplier 110 and a control unit 120, wherein,
the silicon-based photomultiplier 110 is used for acquiring an optical signal emitted from the reaction region; and converting the optical signal into an electrical signal; and amplifying the electrical signal to generate an amplified electrical signal;
the control unit 120 is used for controlling the silicon-based photomultiplier 110 to work; and sends the amplified electrical signal to a counting unit.
The control unit 120 can be implemented by the prior art, such as a common control circuit, a micro control module MCU, a control processor CPU, etc., and the control unit 120 is electrically connected to the silicon-based photomultiplier 110.
In the photodetection device 10 of the present application, the light signal emitted from the reaction region is received by the light-sensitive sheet on the silicon-based photomultiplier 110, which has at least the following advantages compared with the prior art: the photon detection device constructed by the silicon-based photomultiplier 110 can obtain high gain under the low working voltage of only 25-30V, and SiPM can accurately obtain an optical signal emitted by a reaction area to be detected without a booster circuit; in addition, in a low-voltage working environment, the safety of the SiPM circuit can be improved, the circuit fault is not easy to occur and the risk of the circuit can be reduced. In addition, the peripheral circuit of the SiPM is simple, so that the usability of the SiPM in acquiring the optical signal emitted by the reaction area to be detected is improved. In addition, the SiPM has a very compact and light structure, the volume is far smaller than that of the PMT, the production cost is far lower than that of the PMT, and the cost of a chemiluminescence detection instrument can be greatly reduced.
In addition, the photodetection device 10 can also convert the optical signal into an electrical signal and amplify the electrical signal, so as to count the optical signal, specifically, the amplified electrical signal can be sent to a counting unit, which can be implemented by the prior art, such as a computer device, a counter, etc.; the number of the electric signals is determined by the counting unit, so that the number of photons corresponding to the optical signals can be obtained, and the detection of the reaction region is completed.
In some embodiments, the counting unit is a portable device, the portable device having pre-stored computer instructions for performing the following: receiving the amplified electrical signal sent by the photoelectric detection device 10, and counting; the portable device may be a laptop, a tablet, a smartphone, or the like.
In some embodiments, the photodetecting device 10 further comprises a heat dissipation mechanism 130. The heat dissipation mechanism 130 is used for dissipating heat of the photodetection device 10. The heat dissipation mechanism 130 may be provided as a heat sink member 131, as shown in fig. 3 or 4.
In other embodiments, the heat dissipation mechanism 130 may also be configured as a refrigerator.
Of course, in still other embodiments, the heat dissipation mechanism 130 may also be configured to include a heat sink member 131 and a refrigerator.
As shown in fig. 3, the heat sink member 131 includes a heat sink element 1311, a heat dissipation channel 1312 for accommodating the silicon-based photomultiplier tube 110. Further, the silicon-based photomultiplier tube 110 is connected to the heat dissipation channel, as shown in fig. 4.
Illustratively, the design of the heat dissipation channel 1312 may be implemented as follows: the heat sink member 131 is recessed from the top end toward the inside to form the heat dissipation channel 1312.
In some embodiments, as shown in fig. 3-4, the fin member 131 further includes a base 1313, and the base 1313 may be disposed at a bottom end of the fin member 131, which is an end opposite the top end of the fin member. Further, the silicon-based photomultiplier 110 is fixed to the base 1313.
In some embodiments, continuing with fig. 4, the top end of the heat dissipation channel 1312 is disposed in an open manner, and the heat sink member 131 further comprises a sealing stopper 1314, wherein the sealing stopper 1314 is disposed at the top end of the heat dissipation channel 1312 for sealing the top end of the heat dissipation channel 1312, so as to seal the silicon-based photomultiplier tube 110 inside the heat dissipation channel and provide physical protection for the silicon-based photomultiplier tube 110.
It is easy to understand that, when the photo-detection device 10 is used to detect an optical signal in a reaction region, the heat dissipation channel 1312 faces the reaction region to be detected, which not only facilitates the detection of the optical signal emitted from the reaction region, but also has a certain shielding effect on the light in the non-reaction region, thereby reducing the interference of the environmental background noise.
Third aspect of the invention
As shown in connection with fig. 5-7, a third aspect provides a chemiluminescent photodetecting system 1 comprising:
the microfluidic chip 20 comprises a reaction cell 220, wherein the reaction cell 220 is used for providing a reaction area to be detected; and
the photodetecting device 10 according to the second aspect is used for detecting the optical signal emitted from the reaction region.
It should be noted that the photodetection device 10 is disposed toward the reaction cell 220, and particularly, the silicon-based photomultiplier 110 is disposed toward the reaction cell 220.
When the chemiluminescent photodetection system 1 is used, an object to be detected and a substrate or an excitation liquid are added into the reaction tank 220 to perform a chemiluminescent reaction, and after the chemiluminescent reaction occurs in the reaction tank 220, an emitted optical signal can be received by the silicon-based photomultiplier 110 of the photodetection device 10 and processed correspondingly, so as to finally obtain the number of photons corresponding to the object to be detected, thereby realizing the detection of the object to be detected.
It should be noted that the substance to be detected may be an antigen, a hapten, an antibody, a small molecule, a protein, or the like. The substrate or exciting liquid may be: 1. alkaline phosphatase (ALP, AP) -based substrates such as AMPPD, CDP-star, APS-5, and the like; 2. horseradish peroxidase (HRP) based substrates such as luminol, acridinium ester and derivatives thereof, and the like; 3. an excitation liquid based on luminol and isoluminol and derivatives thereof, acridinium esters and derivatives thereof.
Compared with the prior art, the photoelectric detection system 1 of the present application combines the characteristic of the small volume of the microfluidic chip 20 with the characteristic of the photoelectric detection device 10 described in the second aspect (the introduction of the photoelectric detection device 10 is referred to above), which not only can consider the detection cost and the detection sensitivity, but also can further reduce the volume of the photoelectric detection system 1, and improve the application range.
However, if the existing chemiluminescence reaction device except the microfluidic chip 20 is combined with the photodetection device 10 according to the second aspect, the beneficial effects of the photodetection system 1 according to the present application cannot be achieved. For this reason, the conventional chemiluminescent reaction device generally comprises a sample adding module, an incubation reaction module, a washing module and a substrate or exciting liquid injecting module. The working process of the existing chemiluminescence reaction device is roughly as follows: the sample adding module comprises a reaction cup, a sample needle is used for sequentially adding a substance to be detected and a corresponding reaction reagent into the reaction cup, then an immunoreaction process is completed in the incubation module to obtain an immunoreaction compound, then the reactant is cleaned in the cleaning module under the action of an external magnetic field to remove unreacted substances and other interferents, and finally a substrate or an excitation liquid is added under the substrate or excitation liquid injection module to generate an optical signal. As apparent from this, the conventional chemiluminescent reaction apparatus includes a plurality of modules, and it is difficult to effectively simplify the volume.
In some embodiments, the chemiluminescent reaction occurring within the reaction cell of the microfluidic chip 20 is a homogeneous chemical reaction. When the chemiluminescent reaction in the reaction cell 220 is a homogeneous phase reaction, the reactants in the reaction cell 220 do not need to be cleaned, and a good detection reaction effect can be obtained. Therefore, a module structure related to cleaning reactants can be omitted, the size of the photoelectric detection system 1 is further reduced, and the detection cost is reduced.
In some embodiments, the chemiluminescent reaction in the reaction cell 220 of the microfluidic chip 20 is a semi-homogeneous chemical reaction, and the microfluidic chip 20 is provided with magnetic beads and a magnetic bead channel for placing the magnetic beads;
the photoelectric detection system 1 further comprises a magnet module, and the magnet module is used for collecting and dragging magnetic beads in the magnetic bead channel. The arrangement of the magnet module, the magnetic beads and the bead channels for placing the magnetic beads is described in patent document No. 2018219906897 entitled "a microfluidic chemiluminescent immunoassay analyzer".
In some embodiments, the chemiluminescent reaction occurring in the reaction cell 220 of the microfluidic chip 20 is a heterogeneous chemical reaction, and the microfluidic chip 20 is directly or indirectly coated with a protein or nucleic acid for specific binding to the analyte to be detected;
the photoelectric detection system 1 further comprises an air pump, and the air pump is used for providing power required by the movement of liquid in the micro-channel of the micro-fluidic chip 20.
In some embodiments, the photo detection system 1 further comprises a temperature control device 40, wherein the temperature control device 40 is used for adjusting the temperature of the reaction cell 220. The temperature control device 40 can be implemented by a conventional technique.
In some embodiments, the microfluidic chip 20 includes a carrier member 210; the carrying component 210 is used for adding an object to be detected, a substrate or an excitation liquid into the reaction tank 220; it should be noted that, in some embodiments, the substance to be detected, the substrate or the excitation liquid may also be added into the reaction cell 220 through other embodiments, and specifically, the setting may be performed by a person skilled in the art according to actual needs;
the photoelectric detection system 1 further comprises a driving motor 30, wherein the driving motor 30 is used for driving the carrying component 210 to move, so that an object to be detected and a substrate or an excitation liquid are added into the reaction tank; specifically, the motor shaft of the driving motor 30 may be dynamically connected to the loading member 210, i.e., the loading member 210 may be driven.
Exemplarily, the carrying component 210 includes a channel 211 for accommodating an object to be detected, a substrate or an excitation liquid, the microfluidic chip 20 further includes a cavity 230 and a flow path 240, the flow path 240 connects the cavity 230 with the reaction cell 220, and the carrying component 210 is slidably connected inside the cavity 230;
when the loading member 210 slides to a predetermined position inside the cavity 230, the channel 211 communicates with the flow path 240, so that the substance to be detected, the substrate or the exciting liquid loaded or contained in the channel 211 can flow into the reaction cell 220.
The flow path 240 may be implemented by a flow channel.
In some embodiments, the microfluidic chip 20 may be configured according to the protocol described in the patent document having application number 202010665566.1 entitled "quantitative liquid feeding device based on time-sequential control, method and microfluidic chip"; the liquid bearing component 210 in the present application may be implemented by the liquid bearing rod described in the patent document, that is, the liquid bearing component 210 is provided as the liquid bearing rod in the patent document.
In some embodiments, as shown in fig. 8, the photo-detection system 1 further includes a portable terminal 50, and the portable terminal 50 is connected to the driving motor 30 for controlling the driving motor 30 to operate. The portable terminal 50 may be a portable computer terminal such as a notebook computer, a tablet computer, a smart phone, and the like.
Further, the portable terminal 50 is also used for providing the counting unit, in other words, the portable terminal 50 is used for receiving the amplified electrical signal emitted by the photodetecting device 10 and counting. More specifically, the portable terminal 50 includes a memory, a processor, and at least one application stored in the memory and configured to be executed by the processor, the at least one application configured to perform the steps of: receiving the amplified electric signal sent by the photoelectric detection device 10 and counting.
When the chemiluminescent photodetection system 1 is used, an object to be detected and a substrate or an excitation liquid are added into the reaction tank 220 to perform a chemiluminescent reaction, and after the chemiluminescent reaction occurs in the reaction tank 220, an emitted optical signal can be received by the silicon-based photomultiplier 110 of the photodetection device 10 and processed correspondingly, so as to finally obtain the number of photons corresponding to the object to be detected, thereby realizing the detection of the object to be detected.
In some embodiments, the portable terminal 50 is communicatively connected to the control unit 120, for example, by a bluetooth module, which increases the convenience of the detection system.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (10)
1. A method for detecting chemiluminescence, which is used to detect an optical signal emitted from a reaction region to be detected, the method comprising:
and acquiring a light signal emitted by the reaction area to be detected by using a silicon-based photomultiplier.
2. The method for detecting chemiluminescence according to claim 1, further comprising:
converting the optical signal into an electrical signal through the silicon-based photomultiplier;
and amplifying the electrical signal on the silicon-based photomultiplier tube to generate an amplified electrical signal.
3. The method for detecting chemiluminescence according to claim 2, further comprising:
and calculating the photon number corresponding to the optical signal according to the amplified electric signal.
4. A chemiluminescent photodetection device, characterized in that it is used to detect the optical signal emitted from the reaction area to be detected, said photodetection device comprising a silicon-based photomultiplier and a control unit, wherein,
the silicon-based photomultiplier is used for acquiring an optical signal emitted by the reaction area; and converting the optical signal into an electrical signal; and amplifying the electrical signal to generate an amplified electrical signal;
the control unit is used for controlling the silicon-based photomultiplier to work and sending the amplified electric signal to a counting unit.
5. A chemiluminescent photodetecting system, characterized by comprising:
the microfluidic chip comprises a reaction pool, wherein the reaction pool is used for providing a reaction area to be detected; and
the chemiluminescent photodetecting device according to claim 4.
6. The chemiluminescent photodetecting system according to claim 5, wherein the chemiluminescent reaction occurring within the reaction cell of the microfluidic chip is a homogeneous immunochemical reaction.
7. The chemiluminescent photodetecting system according to claim 5, wherein the chemiluminescent reaction occurring within the reaction cell of the microfluidic chip is a semi-homogeneous chemical reaction;
the microfluidic chip is provided with magnetic beads and a magnetic bead channel for placing the magnetic beads;
the photoelectric detection system also comprises a magnet module, and the magnet module is used for collecting and dragging the magnetic beads in the magnetic bead channel.
8. The chemiluminescent photodetecting system according to claim 5, characterized in that,
the chemiluminescence reaction generated in the reaction tank of the microfluidic chip is a heterogeneous chemical reaction;
the micro-fluidic chip is provided with protein or nucleic acid for specific binding with an object to be detected;
the photoelectric detection system also comprises an air pump.
9. The chemiluminescent photodetecting system according to claim 5, characterized in that,
the microfluidic chip further comprises a carrying component; the carrying component is used for adding an object to be detected, a substrate or an excitation liquid into the reaction tank;
the photoelectric detection system also comprises a driving motor, and the driving motor is used for driving the carrying component to move so as to add an object to be detected, a substrate or an excitation liquid into the reaction tank.
10. The chemiluminescent photodetecting system according to claim 9, further comprising a portable terminal communicatively connected to the driving motor;
the portable terminal includes a memory, a processor, and at least one application stored in the memory and configured to be executed by the processor, the at least one application configured to perform the steps of: and receiving the amplified electric signal sent by the photoelectric detection device and counting.
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