CN113604345A - Portable clamping device based on nanopore detection chip in magnetic tweezers system - Google Patents
Portable clamping device based on nanopore detection chip in magnetic tweezers system Download PDFInfo
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- CN113604345A CN113604345A CN202110923970.9A CN202110923970A CN113604345A CN 113604345 A CN113604345 A CN 113604345A CN 202110923970 A CN202110923970 A CN 202110923970A CN 113604345 A CN113604345 A CN 113604345A
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- 238000001514 detection method Methods 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 128
- 239000003292 glue Substances 0.000 claims abstract description 12
- 229910052581 Si3N4 Inorganic materials 0.000 claims abstract description 3
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000011521 glass Substances 0.000 claims description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 238000007789 sealing Methods 0.000 abstract description 5
- 238000003860 storage Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 230000008569 process Effects 0.000 description 9
- 238000012856 packing Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000007672 fourth generation sequencing Methods 0.000 description 3
- 238000011179 visual inspection Methods 0.000 description 3
- 238000004026 adhesive bonding Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010040007 Sense of oppression Diseases 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000004557 single molecule detection Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
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- Wood Science & Technology (AREA)
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- Engineering & Computer Science (AREA)
- Microbiology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a portable clamping device based on a nanopore detection chip in a magnetic tweezers system, which comprises an upper left liquid pool, a lower left liquid pool, a C-shaped loading platform, a set screw, a rubber gasket and a right liquid pool, wherein the lower left liquid pool and the right liquid pool are tightly connected and fixed through glue, and the right liquid pool is used as a solution storage container communicated with the lower left liquid pool. And (3) putting the silicon nitride chip between the left lower liquid pool and the left upper liquid pool with the rubber gasket. And meanwhile, the assembled liquid pool is placed on the C-shaped loading platform, so that the side surfaces of the upper left liquid pool and the lower left liquid pool are tightly attached to the side surface of the C-shaped loading platform. The rubber gasket between the chip and the upper and lower liquid pools ensures the sealing connection between the chip and the upper left liquid pool and the lower left liquid pool, and tightly fixes the upper left liquid pool and the lower left liquid pool by rotating four set screws positioned at the upper end of the C-shaped loading platform, so that the upper left liquid pool and the lower left liquid pool are tightly attached. The invention utilizes the design of the combined detachable liquid pool, and can conveniently, quickly, accurately and efficiently realize the positioning and clamping when the liquid pool is connected with the chip, thereby quickly replacing the required chip without damaging the chip.
Description
Technical Field
The invention relates to a chip clamping device, in particular to a portable clamping device based on a nanopore detection chip in a magnetic tweezers system.
Background
With the advent of fourth generation sequencing technologies, nanopores have demonstrated highly sensitive performance in the field of rapid single molecule detection, but have faced many challenges in discriminating between internal bases of DNA. Because the speed of DNA passing through the nanopore is very high, the current change caused by a single base is difficult to capture by the existing instrument at the speed, and sequencing of single DNA passing through the nanopore in a free state cannot be realized, so that a great difficulty of nanopore sequencing is that the speed of DNA passing through the nanopore is effectively reduced. Through the constant search of many research groups, various ways have been sought to reduce the speed of DNA molecules through nanopores. Once the speed of DNA molecules passing through the nanopore is reduced, the obtained data volume is correspondingly improved, and the identification degree of DNA bases is also improved.
Nanopore sequencing is one of the third-generation technologies which hopefully realize high speed, high flux and high accuracy, and provides a mode for controlling DNA to pass through a nanopore by using magnetic tweezers, a set of magnetic tweezers devices is designed, and an experiment platform is built to control the speed of the DNA in the nanopore aiming at the problem that the DNA cannot effectively distinguish bases when the DNA passes through the nanopore at an effective voltage in the third-generation DNA detection of the nanopore. The improvement of experimental instruments and devices and the simplification of experimental steps have very important meanings for improving experimental efficiency and accelerating research process.
At present, a device for clamping a nanopore chip in a specific magnetic tweezers experiment in a laboratory is mainly formed by gluing two acrylic plates with the same size and a groove in the middle through glue, and then punching a small hole in the groove. The clamping steps mainly comprise: 1. uniformly coating PDMS glue on the small holes of the groove of the liquid pool; 2. placing the chip on the small hole stuck in the groove by using tweezers and positioning the chip by visual inspection; 3. cooling for a period of time, observing whether the glue works or not by visual observation, and checking whether water leaks around the chip by using deionized water; 4. the liquid pool is placed on an external bolt frame, and the bolts are tightened to fix the liquid pool.
The device for clamping the nano-holes has several problems in the aspects of convenience in operation, positioning accuracy and reliability: 1. in the aspect of convenience, the old chip is taken away when the chip is replaced every time, and the back surface of the old chip is stuck by glue, so that the old chip can not be used any more. 2. In the aspect of positioning accuracy, no matter the chip or the liquid pool is positioned by visual inspection, although the liquid pool has a concave-convex groove for positioning, a dimension is positioned by visual inspection, and inaccurate positioning may cause failure of an experiment due to larger errors. 3. In the aspect of reliability, when the chip is replaced, the chip is exposed to the external environment, the chip is extremely easy to be polluted or even damaged in the process, and irreparable influence is caused on the experiment. 4. Due to the small size of the chip, the chip needs to be carefully placed in the center of the gasket by tweezers to ensure good sealing and positioning accuracy. Meanwhile, in the process of coating with glue, the coating requirement is uniform, and if the glue is coated too little or not uniformly, the phenomenon of leakage caused by poor sealing property is easy to occur; when the glue is coated too much, the chip and the small hole are not parallel, the chip is not separated well when a new chip is replaced, the old chip is broken, and the surface of the liquid pool is damaged.
The problems make the experimental process complicated, the accuracy of the experimental result is reduced, the operation requirement on personnel is high, the experimental efficiency is greatly influenced, and the research process is slowed down.
Disclosure of Invention
The purpose of the invention is as follows: in order to overcome the defects of the conventional portable clamping device based on the nanopore detection chip in the magnetic tweezers system, the invention provides the portable clamping device based on the nanopore detection chip in the magnetic tweezers system, which is small in size and convenient to carry, can conveniently, quickly, accurately and efficiently complete positioning and clamping when a liquid pool is connected with the chip, simplifies operation steps, shortens chip clamping time, realizes repeated experiments of one chip, and improves experiment efficiency.
The technical scheme is as follows: the invention firstly designs a portable clamping device based on a nanopore detection chip in a magnetic tweezers system, which comprises an upper left liquid pool, a lower left liquid pool, a C-shaped loading platform, a set screw, a rubber gasket and a right liquid pool; the left lower liquid pool and the right liquid pool are tightly connected and fixed, and the left upper liquid pool and the left lower liquid pool are arranged in an up-and-down fit manner; rubber gaskets are arranged on the upper left liquid pool and the lower left liquid pool; putting a silicon nitride chip into a rubber gasket on the left lower liquid pool; and simultaneously placing the assembled liquid pool on a C-shaped loading platform, wherein the side surfaces of the upper left liquid pool and the lower left liquid pool are tightly attached to the side surface of the C-shaped loading platform.
The invention further improves that: the left upper liquid pool, the left lower liquid pool, the right liquid pool, the set screw and the C-shaped loading platform are all made of organic glass PMMA.
The invention further improves that: and the upper left liquid pool and the lower left liquid pool are tightly attached through four fastening screws positioned at the upper end of the C-shaped loading platform in a rotating manner.
The invention further improves that: the left lower liquid pool and the right liquid pool are permanently and tightly attached through glue.
The invention is assembled by three small liquid pools, wherein a left lower liquid pool and a right liquid pool are permanently and tightly attached by glue, and the right liquid pool is used as a solution storage container communicated with the left lower liquid pool. The upper left liquid pool and the lower left liquid pool can be freely assembled and disassembled, a rubber gasket with good sealing property is arranged between the upper left liquid pool and the lower left liquid pool, solution for experiments is firstly injected into the lower left liquid pool and the right liquid pool through needle tubes, a chip is placed on the rubber gasket of the lower left liquid pool, the upper left liquid pool is attached to the lower left liquid pool, the gasket of the upper liquid pool and the gasket of the lower liquid pool are ensured to well wrap the chip, then the whole liquid pool is placed on a C-type loading platform, the upper left liquid pool is closely attached to the lower left liquid pool through rotating 4 set screws on the C-type loading platform, and solution required for experiments is injected into the upper left liquid pool.
Has the advantages that: the invention enables the upper left liquid pool and the lower left liquid pool to clamp the inner nano-pore chip through the pressure of the set screw on the upper left liquid pool, thereby achieving the purpose of quickly and conveniently assembling, disassembling and replacing the required chip in the process of the magnetic tweezers nano-pore detection experiment.
Compared with the prior device, the invention firstly leads the upper left liquid pool and the lower left liquid pool to clamp the tiny chip through 4 fastening screws on the C-shaped loading platform, and the upper left liquid pool and the lower left liquid pool can be directly disassembled and assembled to move and replace the chip. In the location, the rubber packing ring on this novel device accessible liquid pool is fixed a position, and the good leakproofness of liquid pool can be guaranteed to the rubber packing ring for can not leak between liquid pool and the chip, cushion simultaneously about liquid pool and left liquid pool down to the pressure of chip when pressing from both sides tightly, just can take out the chip to the separation that the liquid pool just can be realized to the liquid pool under the chip left side when avoiding pressing from both sides tightly, and the oppression that does not damage the core leads to the chip damaged. In the loading and unloading process, the chip can be used for multiple times only by screwing and loosening the set screw and splitting the upper left liquid pool and the sheet. Compared with the gluing method, the chip can be repeatedly used for many times. The operation is simpler and faster. In reliability, the pollution and even damage to the chip can be greatly reduced. The invention has small volume and is convenient to carry.
By utilizing the novel device, the chip clamping steps can be simplified, the required chip can be quickly replaced in the experimental process, and the positioning accuracy and reliability in the clamping process are improved. The method has important significance for shortening the experimental time and improving the research efficiency.
Drawings
FIG. 1 is a schematic view of a liquid bath structure of the present invention;
FIG. 2 is a cross-sectional view of the upper left fluid bath;
FIG. 3 is a cross-sectional view of the lower left sump;
FIG. 4 is a cross-sectional view of the right reservoir;
FIG. 5 is a top view of the C-type load station;
FIG. 6 is a schematic view of the apparatus assembly;
fig. 7 is an exploded view of the present device.
Detailed Description
The invention will be described in further detail with reference to the following detailed description and accompanying drawings:
as shown in figure 1, the invention comprises a left upper liquid pool 1, a left lower liquid pool 2 and a right liquid pool 3. The left lower liquid pool 2 and the right liquid pool 3 are permanently and tightly connected by 1507 glue.
As shown in fig. 2-5, which are graphs of specific part machining dimensions.
Fig. 6 is an overall assembly drawing and fig. 7 is an exploded drawing, and comprises a left upper liquid pool 1, a left lower liquid pool 2, a C-shaped loading platform 5, a set screw 4, a rubber gasket 6 and a right liquid pool 5.
Four holding screws 4 on the C type loading platform 5 and 4 screw holes on the C type loading platform are connected through screw threads, and through rotating the four holding screws, the upper left liquid pool 1 and the lower left liquid pool 2 placed on the C type loading platform can be tightly pressed, and the good sealing property between the upper left liquid pool and the lower left liquid pool is ensured.
During the use, at first with the needle tubing with the solution of experiment pour into left lower liquid pool 2 and right liquid pool 3 into, place the chip on the rubber packing ring 6 of liquid pool 2 under the left side, reuse upper left liquid pool 1 laminate to liquid pool 2 under the left side, guarantee that the rubber packing ring 6 of liquid pool 2 wraps up the chip well from top to bottom, then place whole liquid pool in C type loading platform 5, through 4 holding screw 4 on the rotatory C type loading platform with upper left liquid pool 1 closely laminate on liquid pool 2 under the left side, pour into the solution that the experiment needs into in upper left liquid pool 1 again.
Claims (4)
1. Portable clamping device based on nanopore detects chip in magnetic tweezers system, its characterized in that: comprises a left upper liquid pool (1), a left lower liquid pool (2), a C-shaped loading platform (5), a set screw (4), a rubber gasket (6) and a right liquid pool (3); the left lower liquid pool (2) and the right liquid pool (3) are tightly connected and fixed, and the left upper liquid pool (1) and the left lower liquid pool (2) are vertically attached and arranged; rubber gaskets (6) are respectively arranged on the upper left liquid pool (1) and the lower left liquid pool (2); putting a silicon nitride chip into a rubber gasket (6) on the left lower liquid pool (2); and meanwhile, placing the assembled liquid pool on a C-shaped loading platform (5), wherein the side surfaces of the upper left liquid pool (1) and the lower left liquid pool (2) are tightly attached to the side surface of the C-shaped loading platform (5).
2. The portable clamping device based on the nanopore detection chip in the magnetic tweezers system of claim 1, wherein: the left upper liquid pool (1), the left lower liquid pool (2), the right liquid pool (3), the set screw (4) and the C-shaped loading platform (5) are all made of organic glass PMMA.
3. The portable clamping device based on the nanopore detection chip in the magnetic tweezers system according to any claim 1 to 3, wherein: the upper left liquid pool (1) and the lower left liquid pool (2) are both provided with rubber gaskets (6), and the upper left liquid pool (1) and the lower left liquid pool (2) can be tightly attached through four fastening screws (4) which are rotatably positioned at the upper end of the C-shaped loading platform (5).
4. The portable clamping device based on the nanopore detection chip in the magnetic tweezers system of claim 1, wherein: the left lower liquid pool (2) and the right liquid pool (3) are permanently and tightly attached through glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110923970.9A CN113604345B (en) | 2021-08-12 | 2021-08-12 | Portable clamping device based on nanopore detection chip in magnetic tweezer system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110923970.9A CN113604345B (en) | 2021-08-12 | 2021-08-12 | Portable clamping device based on nanopore detection chip in magnetic tweezer system |
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| CN113604345A true CN113604345A (en) | 2021-11-05 |
| CN113604345B CN113604345B (en) | 2024-04-12 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164087A (en) * | 2021-11-26 | 2022-03-11 | 东南大学 | Portable chip clamping device |
| CN114705743A (en) * | 2022-04-01 | 2022-07-05 | 南京师范大学 | Experimental device and experimental method based on nanopores |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002340911A (en) * | 2001-05-15 | 2002-11-27 | Hitachi Ltd | Sheet-type microreactor and mobile type chemical analyzer |
| EP1281440A1 (en) * | 2001-07-30 | 2003-02-05 | F. Hoffmann-La Roche Ag | Device for receiving a chip shaped carrier and process for assembling a plurality of such devices |
| JP2003172736A (en) * | 2001-12-07 | 2003-06-20 | Toyo Kohan Co Ltd | Microchip for chemical reaction |
| CN111088154A (en) * | 2019-12-25 | 2020-05-01 | 广东工业大学 | Graphene nanopore sequencer and sequencing method thereof |
-
2021
- 2021-08-12 CN CN202110923970.9A patent/CN113604345B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002340911A (en) * | 2001-05-15 | 2002-11-27 | Hitachi Ltd | Sheet-type microreactor and mobile type chemical analyzer |
| EP1281440A1 (en) * | 2001-07-30 | 2003-02-05 | F. Hoffmann-La Roche Ag | Device for receiving a chip shaped carrier and process for assembling a plurality of such devices |
| JP2003172736A (en) * | 2001-12-07 | 2003-06-20 | Toyo Kohan Co Ltd | Microchip for chemical reaction |
| CN111088154A (en) * | 2019-12-25 | 2020-05-01 | 广东工业大学 | Graphene nanopore sequencer and sequencing method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164087A (en) * | 2021-11-26 | 2022-03-11 | 东南大学 | Portable chip clamping device |
| CN114705743A (en) * | 2022-04-01 | 2022-07-05 | 南京师范大学 | Experimental device and experimental method based on nanopores |
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| CN113604345B (en) | 2024-04-12 |
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