CN113604338A - Microbial preparation inoculation equipment capable of realizing batch production for bioengineering - Google Patents
Microbial preparation inoculation equipment capable of realizing batch production for bioengineering Download PDFInfo
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- CN113604338A CN113604338A CN202110897822.4A CN202110897822A CN113604338A CN 113604338 A CN113604338 A CN 113604338A CN 202110897822 A CN202110897822 A CN 202110897822A CN 113604338 A CN113604338 A CN 113604338A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 230000000813 microbial effect Effects 0.000 title claims abstract description 20
- 238000010923 batch production Methods 0.000 title description 6
- 230000007246 mechanism Effects 0.000 claims abstract description 66
- 241000894006 Bacteria Species 0.000 claims abstract description 43
- 241000233866 Fungi Species 0.000 claims abstract description 23
- 238000003466 welding Methods 0.000 claims abstract description 4
- 238000007790 scraping Methods 0.000 claims description 31
- 238000003825 pressing Methods 0.000 claims description 28
- 230000002159 abnormal effect Effects 0.000 claims description 14
- 230000005540 biological transmission Effects 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 8
- 230000006835 compression Effects 0.000 claims description 7
- 238000007906 compression Methods 0.000 claims description 7
- 230000001788 irregular Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
The invention relates to microbial preparation inoculation equipment, in particular to microbial preparation inoculation equipment which is used for bioengineering and can be produced in batches, and the microbial preparation inoculation equipment comprises a bottom plate, a trapezoidal guide groove frame, a special-shaped rotating frame, a special-shaped support frame, a reciprocating driving mechanism and the like; the bottom plate bottom surface fixed mounting has trapezoidal guide way frame, has trapezoidal spout on the trapezoidal guide way frame, and the welding of bottom plate top surface has heterotypic rotating turret, and the same fixed mounting of heterotypic support frame is at the bottom plate top surface, and reciprocal actuating mechanism locates trapezoidal guide way frame top. Promote heterotypic carriage and device on it through fluting carriage second and move towards being close to heterotypic support frame direction, make and scrape in the fungus ring stretches into a cultivation test tube, through the effect of nut cover, make the lead screw and scrape fungus ring and rotate, let scrape on the fungus ring and scrape the bacterium sample of getting fully get into in the cultivation test tube, scrape the fungus ring and inoculate the preparation.
Description
Technical Field
The invention relates to microbial preparation inoculation equipment, in particular to microbial preparation inoculation equipment which is used for bioengineering and can be used for batch production.
Background
The microbial preparation is prepared by culturing and expanding the number of microbes beneficial to the growth or disease resistance of crops, and inoculating the microbes on the surfaces of seeds, roots, stems and leaves of the crops to proliferate and act on the roots of the crops, and the living environment of harmful bacteria is inhibited by increasing beneficial bacteria, so that the using purpose is achieved.
When the aseptic operation is carried out, an inoculation sample and a transfer bacterium need to be operated in front of an alcohol burner, an inoculating loop or an inoculating needle needs to burn all metal wires by flame before the inoculation of the bacterium, the joint of the loop, the needle and a rod needs to be burnt, after the bacteria are disinfected by flame, the inoculating loop or the inoculating needle is used for dipping a bacterium sample specimen to inoculate a preparation.
Disclosure of Invention
The invention aims to provide a microbial preparation inoculation device which can pick up a bacterial sample specimen, inoculate a preparation, quickly inoculate bacteria in batches and replace manual operation and is used for bioengineering and batch production, so as to solve the problem that the existing microbial aseptic inoculation technology provided in the background art has more operation steps.
The utility model provides a but be used for bioengineering batch production's microbial preparation inoculation equipment, comprising a base plate, trapezoidal guide way frame, heterotypic rotating turret, abnormal shape support frame, reciprocal actuating mechanism, scrape fungus mechanism, rotatory inoculation mechanism and switching mechanism, bottom plate bottom surface fixed mounting has trapezoidal guide way frame, trapezoidal spout has on the trapezoidal guide way frame, the welding of bottom plate top surface has the heterotypic rotating turret, the same fixed mounting of abnormal shape support frame is at the bottom plate top surface, reciprocal actuating mechanism locates trapezoidal guide way frame top, scrape fungus mechanism fixed mounting at the bottom plate top surface, rotatory inoculation mechanism is located and is scraped on the fungus mechanism, the switching mechanism sets up on abnormal shape support frame.
Further, the reciprocating driving mechanism comprises a push rod fixing block, an electric push rod, a first slotting sliding frame, a second slotting sliding frame, a sliding rail plate, a homing spring, a first reset spring, a limit round bar and a compression spring, the push rod fixing block is welded above the special-shaped support frame, the electric push rod is fixedly arranged on the push rod fixing block, one end of an electric push rod telescopic shaft is welded with the first slotting sliding frame, the first slotting sliding frame is connected with the second slotting sliding frame in a sliding way, the sliding rail plate is fixedly arranged above the trapezoid guide groove frame, the sliding rail plate is connected with the first slotting sliding frame in a sliding way, the homing spring is connected between the first slotting sliding frame and the second slotting sliding frame, the first reset spring is connected on the first slotting sliding frame in a connecting way, one end of the first reset spring is connected with the sliding rail plate, the second slotting sliding frame is connected with the limit round bar in a sliding way, and the limit round bar is connected with the trapezoid guide groove frame in a limiting way, and a compression spring is connected between the limiting round rod and the second slotting sliding frame.
Further, the bacteria scraping mechanism comprises a special-shaped slotted support frame, a rectangular slide rail frame, an extension spring, a reset wedge-shaped frame, a swinging rod, a torsion spring, a special-shaped sliding frame, a second reset spring, a downward pressing sliding block, a third reset spring, a screw rod, a bacteria scraping ring, a slotted wedge-shaped frame, a fourth reset spring, a limit wedge-shaped block and a fifth reset spring, wherein the special-shaped slotted support frame is fixedly arranged on the top surface of the bottom plate, the rectangular slide rail frame is connected on the special-shaped slotted support frame in a lifting manner, the bottom surface of the rectangular slide rail frame is fixedly connected with the extension spring, one end of the extension spring is fixedly connected with the special-shaped slotted support frame, the reset wedge-shaped frame is fixedly arranged on the special-shaped slotted support frame, the swinging rod is rotatably connected on the reset wedge-shaped frame, the torsion spring is connected between the reset wedge-shaped frame and the swinging rod, the special-shaped sliding frame is slidably connected on the rectangular slide rail frame, and the special-shaped sliding frame is contacted with the slotted sliding frame, a second reset spring is fixedly connected to one side of the special-shaped sliding frame, one end of the second reset spring is connected with the rectangular sliding frame, a downward pressing sliding block is connected to the special-shaped sliding frame in a liftable mode, the downward pressing sliding block is in contact with the second slotting sliding frame, the bottom surface of the downward pressing sliding block is connected with a third reset spring, one end of the third reset spring is connected with the special-shaped sliding frame, a lead screw is rotatably connected to the downward pressing sliding block, one end of the lead screw is welded with a scraping ring, a slotted wedge frame is slidably connected to the special-shaped slotted support frame, the slotted wedge frame is in limit connection with the trapezoidal guide slot frame, a fourth reset spring is connected to the slotted wedge frame, one end of the fourth reset spring is connected with the special-shaped slotted support frame, a limit wedge is slidably connected to the slotted wedge frame, the limit wedge is in contact with the rectangular sliding frame, and a fifth reset spring is connected between the limit wedge frame and the slotted wedge frame.
The rotary inoculation mechanism comprises a special-shaped slotted base frame, slotted lower pressure rods, sixth reset springs, sliding round rod blocks, seventh reset springs and nut sleeves, the special-shaped slotted base frame is symmetrically and fixedly connected to the special-shaped slotted support frame, the two special-shaped slotted base frames are jointly connected with the slotted lower pressure rods in a sliding mode, the slotted lower pressure rods are connected with a pair of sixth reset springs in a connecting mode, one end of each sixth reset spring is fixedly connected with the special-shaped slotted base frame, the sliding round rod blocks are connected onto the special-shaped slotted base frame in a sliding mode, the sliding round rod blocks are in limit fit with the slotted lower pressure rods, one end of each sliding round rod block is fixedly connected with the seventh reset spring, one end of each seventh reset spring is connected with the special-shaped slotted base frame, the nut sleeves are welded to the other ends of the sliding round rod blocks, and threaded grooves corresponding to the screw rods are formed in the nut sleeves.
Further, the switching mechanism comprises a clutch lever, an octagonal rotating frame, a culture test tube, a rectangular slotting frame, a rack, an eighth reset spring, a transmission shaft, a ring, a ninth reset spring, a clutch shaft and a gear, the irregular supporting frame is rotatably connected with the clutch lever, the octagonal rotating frame is fixedly connected onto the clutch lever, the culture test tube is sleeved on the octagonal rotating frame in a distributed manner, the rectangular slotting frame is fixedly installed on the top surface of the bottom plate, the rack is connected onto the rectangular slotting frame in a lifting manner, the eighth reset spring is fixedly connected at the bottom end of the rack, one end of the eighth reset spring is connected with the top surface of the bottom plate, the transmission shaft is rotatably connected onto the irregular rotating frame, the ninth reset spring is fixedly connected onto the irregular rotating frame, one end of the ninth reset spring is connected with the ring, the clutch shaft is slidably connected onto the transmission shaft, the clutch shaft and the clutch lever are mutually engaged, and the ring is rotatably connected with the clutch shaft, the clutch shaft is welded with a gear, and the gear is meshed with the rack.
Further explaining, the device also comprises a shaking mechanism, the shaking mechanism is rotatably connected above the special-shaped support frame and comprises a rotating sleeve, a belt wheel and a belt, the rotating sleeve is rotatably connected above the special-shaped support frame, the belt wheel is arranged on the rotating sleeve, the belt wheel is also arranged on the clutch lever, and the belt is in transmission connection between the two belt wheels.
Further explaining, the device also comprises a heating plate which is fixedly arranged on the top surface of the trapezoidal guide groove frame.
The invention has the beneficial effects that:
the second slotting sliding frame is used for pushing the special-shaped sliding frame and the device on the special-shaped sliding frame to move towards the direction close to the special-shaped support frame, so that the bacteria scraping ring extends into a test tube filled with bacteria samples, the second slotting sliding frame is used for pushing the downward pressing sliding block and the device on the sliding block to move downwards under the action of the trapezoid guide groove frame, and the bacteria scraping ring scrapes the bacteria samples in the test tube.
Promote heterotypic carriage and device on it through fluting carriage second and move towards being close to heterotypic support frame direction, make and scrape in the fungus ring stretches into a cultivation test tube, through the effect of nut cover, make the lead screw and scrape fungus ring and rotate, let scrape on the fungus ring and scrape the bacterium sample of getting fully get into in the cultivation test tube, scrape the fungus ring and inoculate the preparation.
The clutch shaft pushes the clutch rod and the upper device thereof to rotate clockwise, so that the inoculated culture test tube is moved away, and the bacteria scraping ring is convenient to inoculate the preparation in the next culture test tube.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
Fig. 3 is a partial perspective view of the present invention.
Fig. 4 is a schematic perspective view of a part of the reciprocating drive mechanism of the present invention.
Fig. 5 is a partially disassembled perspective view of the reciprocating drive mechanism of the present invention.
FIG. 6 is a schematic view of a first partial body structure of the bacteria scraping mechanism of the present invention.
Fig. 7 is an enlarged schematic view of the structure of the present invention a.
FIG. 8 is a partially disassembled perspective view of the bacteria scraping mechanism of the present invention.
FIG. 9 is a schematic view of a second partial structure of the bacteria scraping mechanism of the present invention.
FIG. 10 is a schematic perspective view of a portion of the rotary inoculating mechanism of the present invention.
FIG. 11 is a schematic view of a rotary inoculating mechanism of the present invention.
Fig. 12 is a schematic view of a first partial body structure of the switching mechanism of the present invention.
Fig. 13 is a schematic view of a second partial body structure of the switching mechanism of the present invention.
Fig. 14 is a schematic perspective view of the shaking mechanism of the present invention.
In the above drawings: 1: bottom plate, 2: trapezoidal guide way frame, 3: special-shaped rotating frame, 4: abnormal shape support frame, 5: reciprocating drive mechanism, 51: push rod fixed block, 52: electric putter, 53: slotted carriage one, 54: grooved sliding frame II, 55: slide plate, 551: homing spring, 56: first return spring, 57: limiting round rod, 58: compression spring, 6: scrape fungus mechanism, 61: profiled slotted support frame, 62: rectangular slide rail frame, 63: tension spring, 64: reset wedge, 65: swing lever, 66: torsion spring, 67: special-shaped sliding frame, 68: second return spring, 69: push-down slider, 610: third return spring, 611: screw rod, 612: scraping bacteria ring, 613: slotted wedge, 614: fourth return spring, 615: limiting wedge block, 616: fifth return spring, 7: rotary inoculation mechanism, 71: irregular slotted base frame, 72: slotted push-down lever, 73: sixth return spring, 74: sliding rod block, 75: seventh return spring, 76: nut sleeve, 8: switching mechanism, 81: clutch lever, 82: octagonal turret, 83: culture tube, 84: rectangular slotted frame, 85: rack, 86: eighth return spring, 87: drive shaft, 88: ring, 89: ninth return spring, 810: clutch shaft, 811: gear, 9: shake even mechanism, 91: rotating sleeve, 92: pulley, 93: belt, 10: heating the plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The utility model provides a but microbial preparation inoculation equipment for bioengineering batch production, as shown in fig. 1-13, including bottom plate 1, trapezoidal guide way frame 2, abnormal shape rotating turret 3, abnormal shape support frame 4, reciprocating drive mechanism 5, scrape fungus mechanism 6, rotatory inoculation mechanism 7 and switching mechanism 8, 1 bottom surface fixed mounting of bottom plate has trapezoidal guide way frame 2, trapezoidal spout has on the trapezoidal guide way frame 2, 1 top surface welding of bottom plate has abnormal shape rotating turret 3, abnormal shape support frame 4 is same fixed mounting at 1 top surface of bottom plate, reciprocating drive mechanism 5 locates trapezoidal guide way frame 2 top, scrape fungus mechanism 6 fixed mounting at 1 top surface of bottom plate, scrape fungus mechanism 6 and be used for scraping the bacterial sample in the test tube and get, rotatory inoculation mechanism 7 for inoculating the bacterium is located and is scraped on fungus mechanism 6, switching mechanism 8 sets up on abnormal shape support frame 4.
The reciprocating driving mechanism 5 comprises a push rod fixing block 51, an electric push rod 52, a first slotted sliding frame 53, a second slotted sliding frame 54, a sliding rail plate 55, a homing spring 551, a first return spring 56, a limiting round rod 57 and a compression spring 58, wherein the push rod fixing block 51 is welded above the special-shaped support frame 4, the electric push rod 52 is fixedly installed on the push rod fixing block 51, the first slotted sliding frame 53 is welded at one end of an expansion shaft of the electric push rod 52 far away from the push rod fixing block 51, the second slotted sliding frame 54 is connected with the first slotted sliding frame 53 in a sliding manner, the sliding rail plate 55 is fixedly installed above the trapezoidal guide slot frame 2, the sliding rail plate 55 is connected with the first slotted sliding frame 53 in a sliding manner, the homing spring 551 is connected between the first slotted sliding frame 53 and the second slotted sliding frame 54, the first return spring 56 is connected on the first slotted sliding frame 53, one end of the first return spring 56 far away from the first slotted sliding frame 53 is connected with the sliding rail plate 55, a limiting round rod 57 is connected with the second slotting sliding frame 54 in a sliding mode, the limiting round rod 57 is connected with the trapezoidal guide groove frame 2 in a limiting mode, and a compression spring 58 is connected between the limiting round rod 57 and the second slotting sliding frame 54.
The bacteria scraping mechanism 6 comprises a special-shaped slotted support frame 61, a rectangular slide rail frame 62, an extension spring 63, a reset wedge-shaped frame 64, a swinging rod 65, a torsion spring 66, a special-shaped sliding frame 67, a second reset spring 68, a downward pressing slide block 69, a third reset spring 610, a screw rod 611, a bacteria scraping ring 612, a slotted wedge-shaped frame 613, a fourth reset spring 614, a limiting wedge-shaped block 615 and a fifth reset spring 616, wherein the special-shaped slotted support frame 61 is fixedly arranged on the top surface of the bottom plate 1 close to the special-shaped rotating frame 3, the rectangular slide rail frame 62 is connected to the special-shaped slotted support frame 61 in a lifting mode, the extension spring 63 is fixedly connected to the bottom surface of the rectangular slide rail frame 62, one end of the extension spring 63 far away from the rectangular slide rail frame 62 is fixedly connected with the special-shaped slotted support frame 61, the reset wedge-shaped frame 64 is fixedly arranged on the special-shaped slotted support frame 61, the swinging rod 65 is rotatably connected to the reset wedge-shaped frame 64, the swing rod 65 is used for guiding the special-shaped sliding frame 67, a torsion spring 66 is connected between the reset wedge-shaped frame 64 and the swing rod 65, the rectangular sliding rail frame 62 is connected with the special-shaped sliding frame 67 in a sliding mode, the special-shaped sliding frame 67 is in contact with the grooved sliding frame II 54, the grooved sliding frame II 54 is used for pushing the special-shaped sliding frame 67 and the upper device thereof to move towards the direction close to the special-shaped support frame 4, one side of the special-shaped sliding frame 67 is fixedly connected with a second reset spring 68, one end of the second reset spring 68 far away from the special-shaped sliding frame 67 is connected with the rectangular sliding rail frame 62, the special-shaped sliding frame 67 is connected with a lower pressing slide block 69 in a lifting mode, the lower pressing slide block 69 is in contact with the grooved sliding frame II 54, the grooved sliding frame II 54 is used for pushing the lower pressing slide block 69 and the upper device thereof to move downwards, the lower pressing slide block 69 is connected with a third reset spring 610, one end of the third reset spring 610 far away from the lower pressing slide block 69 is connected with the special-shaped sliding frame 67, the downward-pressing sliding block 69 is rotatably connected with a screw rod 611, one end of the screw rod 611 far away from the downward-pressing sliding block 69 is welded with a bacteria scraping ring 612, the bacteria scraping ring 612 is used for scraping bacteria samples in the test tube, the special-shaped slotted support frame 61 is slidably connected with a slotted wedge frame 613, the slotted wedge frame 613 is in limited connection with the trapezoidal guide slotted frame 2, the slotted wedge frame 613 is connected with a fourth reset spring 614, one end of the fourth reset spring 614 far away from the slotted wedge frame 613 is connected with the special-shaped slotted support frame 61, the slotted wedge frame 613 is slidably connected with a limiting wedge block 615, the limiting wedge block 615 is in contact with the rectangular slide rail frame 62, the limiting wedge block 615 is used for blocking the rectangular slide rail frame 62, and a fifth reset spring 616 is connected between the limiting wedge block 615 and the slotted wedge frame 613.
The rotary inoculation mechanism 7 comprises a special-shaped slotted base frame 71, slotted lower pressure rods 72, sixth reset springs 73, sliding round rod blocks 74, seventh reset springs 75 and nut sleeves 76, the special-shaped slotted base frame 71 is symmetrically and fixedly connected on the special-shaped slotted support frame 61, the slotted lower pressure rods 72 are jointly and slidably connected on the two special-shaped slotted base frames 71, a pair of sixth reset springs 73 are connected on the slotted lower pressure rods 72, one end of the sixth reset spring 73 far away from the slotted lower pressure rods 72 is fixedly connected with the special-shaped slotted base frame 71, the sliding round rod blocks 74 are slidably connected on the special-shaped slotted base frame 71, the sliding round rod blocks 74 are in limit fit with the slotted lower pressure rods 72, one end of each sliding round rod block 74 is fixedly connected with the seventh reset spring 75, one end of the seventh reset spring 75 far away from the sliding round rod block 74 is connected with the special-shaped slotted base frame 71, one end of each sliding round rod block 74 far away from the seventh reset spring 75 is welded with the nut sleeve 76, the nut case 76 has a thread groove corresponding to the lead screw 611.
The switching mechanism 8 comprises a clutch rod 81, an octagonal rotating frame 82, a culture test tube 83, a rectangular slotting frame 84, a rack 85, an eighth reset spring 86, a transmission shaft 87, a ring 88, a ninth reset spring 89, a clutch shaft 810 and a gear 811, the clutch rod 81 is rotatably connected on the special-shaped supporting frame 4, the octagonal rotating frame 82 is fixedly connected on the clutch rod 81 close to the special-shaped supporting frame 4, the culture test tube 83 is sleeved on the octagonal rotating frame 82 in a distributed manner, the rectangular slotting frame 84 is fixedly installed on the top surface of the bottom plate 1 close to the special-shaped rotating frame 3, the rack 85 is connected on the rectangular slotting frame 84 in a lifting manner, the eighth reset spring 86 is fixedly connected at the bottom end of the rack 85, one end of the eighth reset spring 86 far away from the rack 85 is connected with the top surface of the bottom plate 1, the transmission shaft 87 is rotatably connected on the special-shaped rotating frame 3, the ninth reset spring 89 is fixedly connected on the special-shaped rotating frame 3, a circular ring 88 is connected to one end of a ninth reset spring 89 far away from the special-shaped rotating frame 3, a clutch shaft 810 is connected to the transmission shaft 87 in a sliding mode, the clutch shaft 810 is matched with the clutch rod 81, the circular ring 88 is connected with the clutch shaft 810 in a rotating mode, a gear 811 is welded to the clutch shaft 810, and the gear 811 is meshed with the rack 85.
When the microbial preparation needs to be inoculated, the inoculated preparation is filled in the culture test tube 83, a worker holds the test tube filled with the bacterial sample by hand and aims at the bacteria scraping ring 612, the worker manually controls the electric push rod 52 to contract, the electric push rod 52 contracts to drive the first slotting sliding frame 53 and the upper device thereof to move towards the direction close to the special-shaped support frame 4, the second slotting sliding frame 54 can push the special-shaped sliding frame 67 and the upper device thereof to move towards the direction close to the special-shaped support frame 4, the second reset spring 68 is compressed to enable the bacteria scraping ring 612 to extend into the test tube filled with the bacterial sample, then the limiting circular rod 57 and the upper device thereof move downwards under the action of the trapezoid guide groove frame 2, the second slotting sliding frame 54 pushes the downward pressing block 69 and the upper device thereof to move downwards, and the bacteria scraping ring 612 scrapes the bacterial sample in the test tube.
And then, the operator manually controls the electric push rod 52 to extend, the electric push rod 52 extends to drive the first slotting sliding frame 53 and the upper device thereof to move towards the direction far away from the special-shaped support frame 4, the second slotting sliding frame 54 is separated from the special-shaped sliding frame 67, and the compressed second return spring 68 resets to drive the special-shaped sliding frame 67 and the upper device thereof to move towards the direction far away from the special-shaped support frame 4. Then, the compressed return spring 551 resets to drive the second slot sliding frame 54 to move upwards, the second slot sliding frame 54 no longer pushes the down-pressing sliding block 69 to move downwards, the compressed third return spring 610 resets to drive the down-pressing sliding block 69 and the upper device to move upwards to reset, in the process, the limiting round rod 57 can push the slotted wedge frame 613 and the upper device thereof to move towards the direction away from the special-shaped support frame 4, the fourth reset spring 614 is compressed, the limiting wedge block 615 can not block the rectangular slide rail frame 62 any more, the stretching spring 63 in the stretching state resets to drive the rectangular slide rail frame 62 and the upper device thereof to move downwards, the rectangular slide rail frame 62 moves downwards to be contacted with the slotted lower pressing rod 72, the rectangular slide rail frame 62 pushes the slotted lower pressing rod 72 to move downwards, the slotted lower pressing rod 72 can drive the sliding round rod block 74 and the nut sleeve 76 to move towards each other, and the nut sleeve 76 and the lead screw 611 are mutually matched. When the limiting rod 57 is separated from the slotted wedge frame 613, the compressed fourth return spring 614 is reset to drive the slotted wedge frame 613 and the upper device thereof to move and reset towards the direction close to the special-shaped support frame 4.
The operator controls the electric push rod 52 to contract again, the electric push rod 52 contracts to drive the first slotting sliding frame 53 and the upper device to move towards the direction close to the special-shaped support frame 4, the second slotting sliding frame 54 pushes the special-shaped sliding frame 67 and the upper device to move towards the direction close to the special-shaped support frame 4 again, the bacteria scraping ring 612 extends into the culture test tube 83, the screw rod 611 and the bacteria scraping ring 612 rotate under the action of the nut sleeve 76, and the bacteria scraping ring 612 inoculates the preparation. When the special-shaped sliding frame 67 and the upper device thereof move towards the direction close to the special-shaped support frame 4, the special-shaped sliding frame 67 is contacted with the swinging rod 65, the special-shaped sliding frame 67 pushes the swinging rod 65 to swing, then the special-shaped sliding frame 67 is separated from the swinging rod 65, and the compressed torsion spring 66 resets to drive the swinging rod 65 to swing and reset.
The operator controls the electric push rod 52 to extend again, the electric push rod 52 extends to drive the first slotting carriage 53 and the upper device to move towards the direction far away from the special-shaped support frame 4, the compressed second return spring 68 resets to drive the special-shaped carriage 67 and the upper device to move towards the direction far away from the special-shaped support frame 4, the reset wedge frame 64 blocks the swinging rod 65, the swinging rod 65 guides the special-shaped carriage 67, the reset wedge frame 64 can push the special-shaped carriage 67 and the upper device to move upwards, the stretching spring 63 is stretched, meanwhile, the rectangular slide rail frame 62 is separated from the lower slotting pressure rod 72, the compressed sixth return spring 73 resets to drive the lower slotting pressure rod 72 to move upwards for resetting, the lower slotting pressure rod 72 drives the sliding round rod block 74 to move relatively, and the nut sleeve 76 is separated from the screw rod 611. When the rectangular slide rail frame 62 contacts with the limiting wedge block 615, the rectangular slide rail frame 62 pushes the limiting wedge block 615 to move away from the special-shaped support frame 4, and then the fifth return spring 616 is reset to enable the limiting wedge block 615 to block the rectangular slide rail frame 62.
The rectangular slide rail frame 62 moves downwards to be in contact with the rack 85, the rectangular slide rail frame 62 pushes the rack 85 to move downwards, the rack 85 drives the gear 811 and the upper device to rotate anticlockwise, the clutch lever 81 rotates anticlockwise to push the clutch shaft 810 to move towards the direction far away from the special-shaped support frame 4, and then the compressed ninth reset spring 89 resets to drive the circular ring 88 and the upper device thereof to move towards the direction close to the special-shaped support frame 4 to reset. When the rectangular slide rail frame 62 moves upward to separate from the rack 85, the compressed eighth return spring 86 returns to drive the rack 85 to move upward to return, the rack 85 drives the gear 811 and the upper device thereof to rotate clockwise, and the clutch shaft 810 pushes the clutch lever 81 and the upper device thereof to rotate clockwise, so that the inoculated culture test tube 83 is removed. The above operation is repeated to inoculate the preparation in the culture tubes 83 in batches.
Example 2
On the basis of embodiment 1, as shown in fig. 14, the device further comprises a shaking mechanism 9, the shaking mechanism 9 is rotatably connected above the special-shaped support frame 4, the shaking mechanism 9 is used for enabling the bacteria scraping ring 612 to sufficiently scrape bacteria samples in test tubes, the shaking mechanism 9 comprises a rotating sleeve 91, a belt wheel 92 and a belt 93, the rotating sleeve 91 is rotatably connected above the special-shaped support frame 4, the rotating sleeve 91 is used for placing test tubes containing the bacteria samples, the belt wheel 92 is arranged on the rotating sleeve 91, the belt wheel 92 is also arranged on the clutch lever 81, and the belt 93 is in transmission connection between the two belt wheels 92.
The staff will be equipped with the test tube of bacterium sample and place in rotating cover 91, replace the artifical handheld test tube that is equipped with the bacterium sample, when clutch lever 81 and last device clockwise rotate, band pulley 92 passes through belt 93 and drives and rotate cover 91 and rotate, makes scraping that fungus ring 612 can be abundant scrape the bacterium sample of getting in the test tube, avoids scraping fungus ring 612 and can only scrape the bacterium sample to a place.
Example 3
On the basis of embodiment 2, as shown in fig. 2, a heating plate 10 is further included, the heating plate 10 is fixedly installed on the top surface of the trapezoidal guide groove frame 2, and the heating plate 10 is used for heating the bacteria scraping ring 612.
The heating plate 10 can heat the bacteria scraping ring 612 and sterilize the bacteria scraping ring 612 at high temperature.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A microbial preparation inoculation device which is used for bioengineering and can be produced in batches is characterized in that: including bottom plate (1), trapezoidal guide way frame (2), abnormal shape rotating turret (3), abnormal shape support frame (4), reciprocating drive mechanism (5), scrape fungus mechanism (6), rotatory inoculation mechanism (7) and switching mechanism (8), bottom plate (1) bottom surface fixed mounting has trapezoidal guide way frame (2), trapezoidal spout has on trapezoidal guide way frame (2), bottom plate (1) top surface welding has abnormal shape rotating turret (3), the same fixed mounting of abnormal shape support frame (4) is at bottom plate (1) top surface, trapezoidal guide way frame (2) top is located in reciprocating drive mechanism (5), scrape fungus mechanism (6) fixed mounting at bottom plate (1) top surface, rotatory inoculation mechanism (7) are located and are scraped on fungus mechanism (6), switching mechanism (8) set up on abnormal shape support frame (4).
2. An inoculating device for bioengineered mass producible microbial agents according to claim 1, wherein: the reciprocating driving mechanism (5) comprises a push rod fixing block (51), an electric push rod (52), a first slotted sliding frame (53), a second slotted sliding frame (54), a sliding rail plate (55), a homing spring (551), a first reset spring (56), a limiting round rod (57) and a compression spring (58), the push rod fixing block (51) is welded above the special-shaped support frame (4), the electric push rod (52) is fixedly installed on the push rod fixing block (51), the first slotted sliding frame (53) is welded at one end of an expansion shaft of the electric push rod (52), the second slotted sliding frame (54) is connected on the first slotted sliding frame (53) in a sliding mode, the sliding rail plate (55) is fixedly installed above the trapezoidal guide groove frame (2), the sliding rail plate (55) is connected with the first slotted sliding frame (53) in a sliding mode, the homing spring (551) is connected between the first slotted sliding frame (53) and the second slotted sliding frame (54), a first return spring (56) is connected to the first slotting sliding frame (53), one end of the first return spring (56) is connected with the sliding rail plate (55), a limiting round rod (57) is connected to the second slotting sliding frame (54) in a sliding mode, the limiting round rod (57) is connected with the trapezoidal guide groove frame (2) in a limiting mode, and a compression spring (58) is connected between the limiting round rod (57) and the second slotting sliding frame (54).
3. An inoculating device for bioengineered mass producible microbial agents according to claim 2, wherein: the fungus scraping mechanism (6) comprises a special-shaped slotted support frame (61), a rectangular slide rail frame (62), an extension spring (63), a reset wedge-shaped frame (64), a swinging rod (65), a torsion spring (66), a special-shaped sliding frame (67), a second reset spring (68), a pressing-down sliding block (69), a third reset spring (610), a lead screw (611), a fungus scraping ring (612), a slotted wedge-shaped frame (613), a fourth reset spring (614), a limiting wedge-shaped block (615) and a fifth reset spring (616), the special-shaped slotted support frame (61) is fixedly arranged on the top surface of the bottom plate (1), the rectangular slide rail frame (62) is connected on the special-shaped slotted support frame (61) in a lifting manner, the extension spring (63) is fixedly connected to the bottom surface of the rectangular slide rail frame (62), one end of the extension spring (63) is fixedly connected with the special-shaped slotted support frame (61), the reset wedge-shaped frame (64) is fixedly arranged on the special-shaped slotted support frame (61), the swing rod (65) is rotatably connected to the reset wedge-shaped frame (64), a torsion spring (66) is connected between the reset wedge-shaped frame (64) and the swing rod (65), the rectangular slide rail frame (62) is slidably connected with a special-shaped sliding frame (67), the special-shaped sliding frame (67) is in contact with the second slotted sliding frame (54), one side of the special-shaped sliding frame (67) is fixedly connected with a second reset spring (68), one end of the second reset spring (68) is connected with the rectangular slide rail frame (62), the special-shaped sliding frame (67) is connected with a downward pressing sliding block (69) in a lifting mode, the downward pressing sliding block (69) is in contact with the second slotted sliding frame (54), the bottom surface of the downward pressing sliding block (69) is connected with a third reset spring (610), one end of the third reset spring sliding frame (610) is connected with the special-shaped sliding frame (67), the upward pressing sliding block (69) is rotatably connected with a screw rod (611), the special-shaped grooving wedge-shaped frame is characterized in that a bacterium scraping ring (612) is welded at one end of a screw rod (611), a grooving wedge-shaped frame (613) is connected to a special-shaped grooving support frame (61) in a sliding mode, the grooving wedge-shaped frame (613) is in limiting connection with a trapezoidal guide groove frame (2), a fourth reset spring (614) is connected to the grooving wedge-shaped frame (613), one end of the fourth reset spring (614) is connected with the special-shaped grooving support frame (61), a limiting wedge block (615) is connected to the grooving wedge-shaped frame (613) in a sliding mode, the limiting wedge block (615) is in contact with a rectangular sliding rail frame (62), and a fifth reset spring (616) is connected between the limiting wedge block (615) and the grooving wedge-shaped frame (613).
4. An inoculating device for bioengineered mass producible microbial agents according to claim 3, wherein: the rotary inoculation mechanism (7) comprises a special-shaped slotted base frame (71), a slotted lower pressing rod (72), a sixth reset spring (73), a sliding circular rod block (74), a seventh reset spring (75) and a nut sleeve (76), the special-shaped slotted base frame (71) is symmetrically and fixedly connected to the special-shaped slotted support frame (61), the two special-shaped slotted base frames (71) are jointly and slidably connected with the slotted lower pressing rod (72), the slotted lower pressing rod (72) is connected with a pair of sixth reset springs (73), one end of each sixth reset spring (73) is fixedly connected with the special-shaped slotted base frame (71), the special-shaped slotted base frame (71) is slidably connected with the sliding circular rod block (74), the sliding circular rod block (74) is in limit fit with the slotted lower pressing rod (72), one end of the sliding circular rod block (74) is fixedly connected with the seventh reset spring (75), one end of the seventh reset spring (75) is connected with the special-shaped slotted base frame (71), the other end of the sliding round rod block (74) is welded with a nut sleeve (76), and the nut sleeve (76) is provided with a thread groove corresponding to the screw rod (611).
5. An inoculating device for bioengineered mass producible microbial agents according to claim 4, wherein: the switching mechanism (8) comprises a clutch lever (81), an octagonal rotating frame (82), a culture test tube (83), a rectangular slotted frame (84), a rack (85), an eighth reset spring (86), a transmission shaft (87), a circular ring (88), a ninth reset spring (89), a clutch shaft (810) and a gear (811), the irregular supporting frame (4) is rotatably connected with the clutch lever (81), the octagonal rotating frame (82) is fixedly connected on the clutch lever (81), the culture test tube (83) is sleeved on the octagonal rotating frame (82) in a distributed manner, the rectangular slotted frame (84) is fixedly installed on the top surface of the bottom plate (1), the rectangular slotted frame (84) is connected with the rack (85) in a liftable manner, the eighth reset spring (86) is fixedly connected at the bottom end of the rack (85), one end of the eighth reset spring (86) is connected with the top surface of the bottom plate (1), the irregular rotating frame (3) is rotatably connected with the transmission shaft (87), the special-shaped rotating frame (3) is fixedly connected with a ninth reset spring (89), one end of the ninth reset spring (89) is connected with a ring (88), a transmission shaft (87) is connected with a clutch shaft (810) in a sliding mode, the clutch shaft (810) is mutually matched with a clutch rod (81), the ring (88) is rotatably connected with the clutch shaft (810), a gear (811) is welded on the clutch shaft (810), and the gear (811) is meshed with a rack (85).
6. An inoculating device for bioengineered mass producible microbial agents according to claim 5, wherein: the automatic shaking device is characterized by further comprising a shaking mechanism (9), the shaking mechanism (9) is rotatably connected above the special-shaped support frame (4), the shaking mechanism (9) comprises a rotating sleeve (91), a belt wheel (92) and a belt (93), the rotating sleeve (91) is rotatably connected above the special-shaped support frame (4), the belt wheel (92) is arranged on the rotating sleeve (91), the belt wheel (92) is also arranged on the clutch lever (81), and the belt (93) is in transmission connection between the two belt wheels (92).
7. An inoculating device for bioengineered mass producible microbial agents according to claim 6, wherein: the heating plate (10) is fixedly arranged on the top surface of the trapezoid guide groove frame (2).
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| CN202110897822.4A CN113604338A (en) | 2021-08-05 | 2021-08-05 | Microbial preparation inoculation equipment capable of realizing batch production for bioengineering |
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| CN202110897822.4A CN113604338A (en) | 2021-08-05 | 2021-08-05 | Microbial preparation inoculation equipment capable of realizing batch production for bioengineering |
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| WO2014188113A1 (en) * | 2013-05-24 | 2014-11-27 | Intelligence Artificielle Applications | Device for the automated seeding of culture medium |
| CN113005020A (en) * | 2021-03-20 | 2021-06-22 | 周桂珍 | Microbial bacteria culture and propagation inoculation instrument |
| JP2021108644A (en) * | 2020-01-08 | 2021-08-02 | 胡金霞 | Inoculation device using microbiological assay |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050072983A (en) * | 2004-01-08 | 2005-07-13 | 주식회사파멕스 | inoculating machine of the fungi, etc. |
| KR101026910B1 (en) * | 2010-09-10 | 2011-04-04 | 신근영 | Linear machine for filling mushroom culturing medium |
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| WO2014188113A1 (en) * | 2013-05-24 | 2014-11-27 | Intelligence Artificielle Applications | Device for the automated seeding of culture medium |
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