CN113588825B - Rapid identification method for fingerprint spectrum of radix paeoniae alba genuine medicinal material - Google Patents
Rapid identification method for fingerprint spectrum of radix paeoniae alba genuine medicinal material Download PDFInfo
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- CN113588825B CN113588825B CN202110872483.4A CN202110872483A CN113588825B CN 113588825 B CN113588825 B CN 113588825B CN 202110872483 A CN202110872483 A CN 202110872483A CN 113588825 B CN113588825 B CN 113588825B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
Abstract
The invention relates to the technical field of medicine detection, in particular to a method for quickly identifying a fingerprint of a radix paeoniae alba genuine medicinal material, which comprises the following steps of (1) preparing a test solution: grinding proper amount of radix Paeoniae alba genuine medicinal materials into fine powder, and extracting with methanol solution to obtain test solution; (2) preparation of mixed reference solution: precisely weighing 3 reference substances including penoniflorin, albiflorin and hydroxy paeoniflorin, and adding methanol to obtain mixed reference substance solution; (3) measuring: precisely measuring the test solution prepared in the step (1) and the reference solution prepared in the step (2) respectively, injecting into a high performance liquid chromatograph, measuring by gradient elution, and recording a chromatogram; processing the obtained fingerprint by using fingerprint software to obtain the fingerprint of the radix paeoniae alba genuine medicinal material, and the fingerprint has the advantages of rapidness, stability, high precision, strong reproducibility and the like; the contrast products paeoniflorin, albiflorin and hydroxyl paeoniflorin have short peak time, and can greatly shorten the detection time.
Description
Technical Field
The invention relates to the technical field of medicine detection, in particular to a method for quickly identifying a fingerprint of a radix paeoniae alba genuine medicinal material.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
The existing quantitative evaluation method of traditional Chinese medicine utilizes a spectrum or chromatographic means to identify and measure one or more effective components, active components or index components, and most varieties only have general inspection items. The traditional Chinese medicine has complex components and diverse and complex chemical components, and is always the key point and the difficulty of quality evaluation.
Radix Paeoniae alba is dry root of Paeonia lactiflora pall of Ranunculaceae, and is bitter and sour in nature and enters liver and spleen channels. Has effects of suppressing hyperactive liver, relieving pain, nourishing blood, regulating menstruation, astringing yin, and arresting sweating. It is used to treat headache, vertigo, abdominal pain, contracture and pain of limbs, blood deficiency, sallow complexion, menoxenia, spontaneous perspiration, and night sweat.
White peony root is mainly produced in Bo Zhou, vortex yang, linquan, chuan and Feng Tai in Anhui; zhongjiang, cangxi, quxian, xuanhan, yilong, guangan in Sichuan; zhejiang Dongyang, yongkang, xianju, lin' an, anji, shandong, henan, hunan. Wherein Anhui Mazhou is the genuine producing area of white peony root. It is called "Bai Shao".
The prior art CN109632991A discloses a method for identifying white peony root and wine white peony root by using HPLC characteristic spectrum detection, which establishes characteristic spectrums of white peony root decoction pieces and wine white peony root decoction pieces, and determines whether a white peony root sample is stir-fried with wine or not, the method can effectively, accurately and characteristically identify the difference between the white peony root and the wine white peony root decoction pieces, but cannot accurately investigate the white peony root in different producing areas; the prior art CN105866296A discloses a fingerprint method of a radix paeoniae alba pharmaceutical preparation, which establishes a fingerprint of the radix paeoniae alba pharmaceutical preparation, determines 4 common characteristic peaks, and identifies three components: respectively hydroxy paeoniflorin, albiflorin and paeoniflorin; the fingerprint provided by the invention has fewer characteristic peaks, bad peak shape and trailing individual peaks; in the prior art, high performance liquid chromatography and thin-layer chromatography fingerprints of a white paeony root medicinal material are shared in fingerprint researches of white paeony root medicinal materials in different producing areas, wherein the thin-layer chromatography condition is that a paeoniflorin reference solution and a test solution are respectively spotted on the same silica gel G thin-layer plate and are developed by taking trichloromethane-ethyl acetate-methanol-formic acid as a developing agent.
Although the existing fingerprint detection method for white peony root can realize the identification of white peony root producing area or the evaluation of white peony root quality, most of the fingerprint detection methods for white peony root in the prior art have long detection time and consume time and labor for detection.
Disclosure of Invention
The white paeony root is used as a raw material medicine of neck pain series medicines such as neck pain granules, neck pain tablets, neck pain capsules and the like, has very large use amount and is very important for controlling the quality. When the single component is used as the quality control index of the white paeony root, the inherent quality of the white paeony root is difficult to be comprehensively controlled, and the authenticity and the quality of the white paeony root cannot be judged; however, when multiple components are used as the quality control indexes of the white paeony root, the peak emergence time of most fingerprint spectrum detection methods in the prior art is long, so that the time for detecting a large batch of white paeony root samples is very long;
therefore, through the selection of the components of the reference substance and the optimization of the liquid chromatography elution procedure, the fingerprint detection method disclosed by the invention can comprehensively characterize the quality of the white paeony root, and can shorten the peak-off time, so that the fingerprint detection method of the ligusticum wallichii is more convenient and quicker.
Based on the technical problems in the prior art, the invention aims to provide a method for quickly identifying the fingerprint of the genuine radix paeoniae alba medicinal material, and compared with the method for identifying the fingerprint of the radix paeoniae alba medicinal material in the prior art, the method has the advantages of shorter peak time and more convenient and quicker detection.
Specifically, the technical scheme of the invention is as follows:
in a first aspect of the invention, a method for rapidly identifying a fingerprint of a radix paeoniae alba genuine medicinal material is provided, which comprises the following steps:
(1) Preparing a test solution: grinding proper amount of radix Paeoniae alba genuine medicinal materials into fine powder, ultrasonically extracting with pure water, ultrasonically extracting with methanol, and mixing extractive solutions to obtain test solution;
(2) Preparation of mixed control solution: precisely weighing 3 reference substances including penoniflorin, albiflorin and hydroxy paeoniflorin, and adding methanol to obtain mixed reference substance solution;
(3) And (3) determination: precisely measuring the test solution prepared in the step (1) and the reference solution prepared in the step (2) respectively, injecting into a high performance liquid chromatograph for determination, wherein the mobile phase is 0.1% phosphoric acid aqueous solution-acetonitrile, performing gradient elution, and recording a chromatogram; processing the obtained fingerprint by fingerprint software to obtain the fingerprint of the radix paeoniae alba genuine medicinal material.
In a second aspect of the present invention, the present invention provides a rapid identification method according to the first aspect and/or an application of the fingerprint obtained by the rapid identification method according to the first aspect in quality control of the genuine herbs of paeonia lactiflora.
In a third aspect of the present invention, a quality control method for radix paeoniae alba genuine medicinal materials is provided, which comprises the following steps:
(1) Taking multiple batches of radix paeoniae alba genuine medicinal materials to obtain fingerprint spectrums of radix paeoniae alba genuine medicinal material samples according to the rapid identification method, and generating the fingerprint spectrums of the multiple batches of radix paeoniae alba genuine medicinal material samples according to an averaging method to obtain a comparison fingerprint spectrum;
preparing a sample to be detected into a sample solution, and operating according to the steps of the rapid identification method to obtain a fingerprint of the sample to be detected;
(2) And (3) carrying out similarity comparison on the fingerprint of the sample to be detected and the radix paeoniae alba genuine medicinal material comparison fingerprint to evaluate the quality of the radix paeoniae alba genuine medicinal material to be detected, wherein the calculation method is a correlation coefficient method.
The specific embodiment of the invention has the following beneficial effects:
in the specific embodiment of the invention, 3 glycoside components of paeoniflorin, albiflorin and hydroxy paeoniflorin are used as reference substances, the coverage is wide, the representativeness is strong, the constructed fingerprint can more comprehensively reflect the quality information of the genuine medicinal material of the white paeony root, and the invention has the advantages of rapidness, stability, high precision, strong reproducibility and the like, and can be used for quality control of the preparation;
the chromatographic peaks of the reference products paeoniflorin, albiflorin and hydroxy paeoniflorin all peak within the first 15 minutes of elution, the peak-off time is short, which is very favorable for the condition that a large amount of white paeony root samples need to be detected, the detection time can be greatly shortened, time and labor are saved, and the time cost and the economic cost of large-scale detection are reduced;
the quality of the radix paeoniae alba genuine medicinal materials can be comprehensively characterized, the production and clinical medication of the products can be guided, and the safety and the effectiveness of the clinical medication can be ensured; the method is simple and convenient, and has good reproducibility.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a fingerprint of 10 batches of genuine white peony root medicinal material series in example 1 of the present invention;
fig. 2 is a fingerprint of a paeonia lactiflora genuine medicinal material product in example 1 of the invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As discussed in the background art, the inventor researches and discovers that the fingerprint characteristic peaks established in the prior art are difficult to comprehensively reflect the overall quality characteristics of the product, and the quality of the product cannot be controlled as a whole. In view of the above, the invention provides a method for constructing a fingerprint spectrum of a radix paeoniae alba genuine medicinal material and application thereof.
The invention provides a method for rapidly identifying a fingerprint of a radix paeoniae alba genuine medicinal material, which comprises the following steps:
(1) Preparing a test solution: grinding proper amount of radix Paeoniae alba genuine medicinal materials into fine powder, and extracting with methanol solution to obtain test solution;
(2) Preparation of mixed control solution: precisely weighing 3 reference substances including penoniflorin, albiflorin and hydroxy paeoniflorin, and adding methanol to obtain mixed reference substance solution;
(3) And (3) determination: precisely measuring the test solution prepared in the step (1) and the reference solution prepared in the step (2) respectively, injecting into a high performance liquid chromatograph, measuring by gradient elution, and recording a chromatogram; processing the obtained chromatogram by fingerprint chromatogram software to obtain the fingerprint chromatogram of the radix paeoniae alba genuine medicinal material.
Preferably, the preparation of the test solution comprises the following specific steps: grinding proper amount of radix Paeoniae alba, grinding into fine powder, weighing fine powder, adding methanol solution, performing ultrasonic extraction, diluting the clear liquid with methanol to constant volume, filtering with microporous membrane, and collecting filtrate as sample solution;
the preparation method can increase the dissolution amount of effective components in the sample, so that the peak appearance of the subsequent high performance liquid chromatography can be more obvious, and the detection result is more accurate.
Preferably, the conditions of ultrasonic extraction are as follows: the time is 30-40min, the extraction power is 250-300W, and the frequency is 30-40kHz;
preferably, the methanol solution is 50% chromatographic methanol.
Preferably, the chromatographic columns used are: agilent ZORBAX C18 (4.6 mm. Times.250mm, 5 μm);
preferably, the mobile phase: acetonitrile-water (phosphoric acid adjusted to pH 3.0) as mobile phase;
preferably, the gradient elution conditions are: 0 to 5min,10 to 15% A; 5-25min, 15-18% of A; 25-27min, 18% -30% A; 27-38min, 30% -35% A; 38-40min, 35-40% A; 40-60min, 40% -50% A; 60-70min, 50% -65% A.
Preferably, the flow rate of the mobile phase is 1.0ml/min; the column temperature is 35 ℃; the detection wavelength was 230nm, and the amount of sample was 10. Mu.l.
In an embodiment of the present invention, there is provided an application of the above rapid identification method and/or the fingerprint obtained by the rapid identification method of the first aspect in quality control of the paeonia lactiflora koch;
the invention provides a quality control method of a radix paeoniae alba genuine medicinal material, which comprises the following steps:
(1) Taking multiple batches of the radix paeoniae alba genuine medicinal materials to obtain the fingerprint of the radix paeoniae alba genuine medicinal material sample according to the rapid identification method, and generating the fingerprints of the multiple batches of the radix paeoniae alba genuine medicinal material samples according to an averaging method to obtain a reference fingerprint;
preparing a sample to be detected into a sample solution, and operating according to the steps of the rapid identification method to obtain a fingerprint of the sample to be detected;
(2) And (3) carrying out similarity comparison on the fingerprint of the sample to be detected and the radix paeoniae alba genuine medicinal material comparison fingerprint to evaluate the quality of the radix paeoniae alba genuine medicinal material to be detected, wherein the calculation method is a correlation coefficient method.
The invention will be further explained and illustrated with reference to specific examples.
Example 1
Taking 10 different batches of white paeony root medicinal materials purchased from Anhui Maozhou;
1. preparing a test solution: taking the white paeony root, sieving with a 50-mesh sieve to obtain 1g of fine powder, precisely weighing, placing in a conical flask with a plug, precisely adding 25mL of 50% methanol solution, weighing, refluxing in water bath for 30min, standing to normal temperature, weighing again, supplementing the loss amount with 50% methanol solution, shaking up, and filtering through a 0.45-micrometer-sized microporous membrane to obtain a test solution.
2. Preparation of control solutions: weighing appropriate amount of paeoniflorin, albiflorin and hydroxy paeoniflorin, precisely weighing, and adding methanol to obtain solution containing 76 μ g per 1ml as control solution.
3. High performance liquid chromatography for determining fingerprint of white peony root
Examination of mobile phase and gradient elution procedures:
the invention inspects the influence of various flow phases on the peak time of three components of paeoniflorin, albiflorin and hydroxy paeoniflorin in white paeony root, and determines a mobile phase and a gradient elution program which have high peak time and good peak effect on the three components of paeoniflorin, albiflorin and hydroxy paeoniflorin through a gradient elution program.
Respectively injecting 10 batches of white paeony root medicinal material test solution and mixed reference substance solution prepared according to the steps (1) and (2) into a high performance liquid chromatograph under the chromatographic conditions that: waters ARC high performance liquid chromatograph, 2998PDA diode array detector, waters Empower3 workstation; agilent ZORBAX C18 (4.6 mm. Times.250mm, 5 μm) as a column; acetonitrile-water (pH adjusted to 3.0 by phosphoric acid) is used as a mobile phase, the detection wavelength is 230nm, and the flow rate is 1.0ml/min; the gradient elution procedure is shown in the following table; volume flow rate: 1.0mL/min-1, column temperature: the sample size is 10 mu L at 35 ℃, and the fingerprint spectrum is measured.
Gradient elution schedule
| Time (min) | Acetonitrile (%) | Water (phosphoric acid adjusted to pH 3.0) (%) |
| 0 | 10 | 90 |
| 5 | 15 | 85 |
| 25 | 18 | 82 |
| 27 | 30 | 70 |
| 38 | 35 | 65 |
| 40 | 40 | 60 |
| 60 | 50 | 50 |
| 70 | 65 | 35 |
Comparison of fingerprint spectra:
and analyzing the obtained spectrum by using fingerprint spectrum software to obtain the fingerprint spectrum of each batch of the radix paeoniae alba genuine medicinal materials. The chromatogram has 18 common characteristic peaks, and the comparison with the chromatogram of the mixed reference product confirms that the peak 3 is paeoniflorin, the peak 1 is albiflorin and the peak 2 is hydroxy paeoniflorin.
The fingerprint of 10 batches of radix Paeoniae alba genuine medicinal materials is shown in figure 1; introducing the 10 batches of radix Paeoniae alba genuine medicinal material spectra into a Chinese medicinal chromatogram fingerprint similarity evaluation system for software analysis, and performing chromatogram peak matching to generate a fingerprint spectrum as figure 2.
As can be seen from the attached figures 1 and 2, the 3 # peak paeoniflorin, the 1 # peak albiflorin and the 2 # peak are hydroxyl paeoniflorin, chromatographic peaks of the three reference substances all appear within 15 minutes before elution, and the appearance time is short, so that the method is very favorable for the condition that a large quantity of white paeony root samples need to be detected, can greatly shorten the detection time, saves time and labor, and reduces the time cost and the economic cost of large-batch detection.
Example 2
(1) System suitability test and negative interference test
Under the chromatographic conditions, respectively and precisely measuring 10 μ l of each of the test sample solution and the blank solvent, injecting into a liquid chromatograph, and recording the chromatogram. The result shows that the blank solvent HPLC chromatogram does not show a peak at the same retention time position as the paeoniflorin control, does not show a peak at other common peak positions, and has no interference to the determination.
(2) Precision test
Taking the same white paeony root medicinal material, preparing a white paeony root medicinal material test solution according to the method of the step 1 of the embodiment 1, and repeatedly injecting samples for 6 times under the chromatographic conditions of the step 3 of the embodiment 1, wherein the retention time RSD of each chromatographic peak is less than 5 percent. The results show that the precision is good.
(3) Stability test
Preparing a test solution according to the step 1 of the example 1, precisely sucking 10ul of the test solution after the preparation of the test solution is finished, injecting the test solution into a liquid chromatograph, recording the area of a chromatographic peak, measuring the chromatographic peak once every 2 hours, observing the chromatographic peak for 24 hours, calculating the relative retention time of each common peak and the relative standard deviation of the relative peak area, wherein the RSD is less than 2%, and the result shows that the solution stability of each common component in the test solution is good within 24 hours.
(4) Repeatability test
The product was taken, the sample solution was prepared according to the above step 1 of example 1, the measurement was repeated 6 times, and the contents of the respective components in the sample were calculated. The result shows that the relative retention time of all the common peaks and the relative peak area RSD are less than 2 percent, which indicates that the method has good repeatability.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A method for rapidly identifying a fingerprint of a radix paeoniae alba genuine medicinal material is characterized by comprising the following steps:
(1) Preparing a test solution: grinding proper amount of radix Paeoniae alba genuine medicinal materials into fine powder, and extracting with methanol solution to obtain test solution;
(2) Preparation of mixed control solution: precisely weighing 3 reference substances including penoniflorin, albiflorin and hydroxy paeoniflorin, and adding methanol to obtain mixed reference substance solution;
(3) And (3) determination: precisely measuring the test solution prepared in the step (1) and the reference solution prepared in the step (2) respectively, injecting into a high performance liquid chromatograph, measuring by gradient elution, and recording a chromatogram; processing the obtained chromatogram by fingerprint software to obtain the fingerprint of the radix paeoniae alba genuine medicinal material;
the methanol solution is 50% chromatographic methanol;
in the method, the adopted chromatographic column comprises the following steps: agilent ZORBAX C18, specification: 4.6mm × 250mm,5 μm;
in the method, the mobile phase: the phase A is acetonitrile, the phase B is water, and the pH value is adjusted to 3.0 by phosphoric acid;
in the method, the gradient elution conditions are as follows: 0 to 5min,10 to 15% A; 5-25min, 15-18% of A; 25-27min, 18% -30% A; 27-38min, 30% -35% A; 38-40min, 35-40% A; 40-60min, 40-50% A; 60-70min, 50% -65% A;
the flow rate of the mobile phase is 1.0ml/min; the column temperature was 35 ℃; the detection wavelength was 230nm, and the sample volume was 10. Mu.l.
2. The method for rapidly identifying the fingerprint of the genuine radix paeoniae alba as claimed in claim 1, wherein a proper amount of the genuine radix paeoniae alba is taken and ground into fine powder, the fine powder is precisely weighed and added with methanol solution for ultrasonic extraction, the clear solution obtained by ultrasonic extraction is subjected to constant volume with methanol and filtered by a microporous membrane, and the subsequent filtrate is taken as the sample solution.
3. The method for rapidly identifying the fingerprint of the paeonia lactiflora pall genuine medicinal material according to claim 2, wherein the ultrasonic extraction conditions are as follows: the time is 30-40min, the extraction power is 250-300W, and the frequency is 30-40kHz.
4. The method for rapidly identifying the fingerprint of the paeonia lactiflora pall genuine medicinal material and/or the application of the fingerprint obtained by the method for rapidly identifying the fingerprint of the paeonia lactiflora pall genuine medicinal material in quality control of the paeonia lactiflora pall genuine medicinal material in claim 1.
5. A quality control method of radix paeoniae alba genuine medicinal materials is characterized by comprising the following steps:
(1) Taking multiple batches of radix paeoniae alba genuine medicinal materials to obtain fingerprints of radix paeoniae alba genuine medicinal material samples according to the rapid identification method of the radix paeoniae alba genuine medicinal material fingerprints of claim 1, and generating the fingerprints of the multiple batches of radix paeoniae alba genuine medicinal material samples according to an averaging method to obtain a reference fingerprint;
preparing a sample to be detected into a sample solution, and operating according to the steps of the method for quickly identifying the fingerprint of the radix paeoniae alba genuine medicinal material according to claim 1 to obtain the fingerprint of the sample to be detected;
(2) And (3) carrying out similarity comparison on the fingerprint of the sample to be detected and the radix paeoniae alba genuine medicinal material comparison fingerprint to evaluate the quality of the radix paeoniae alba genuine medicinal material to be detected, wherein the calculation method is a correlation coefficient method.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102138985A (en) * | 2011-03-22 | 2011-08-03 | 宁波立华制药有限公司 | Quality control method of total glycosides single preparation of white paeony roots |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138985A (en) * | 2011-03-22 | 2011-08-03 | 宁波立华制药有限公司 | Quality control method of total glycosides single preparation of white paeony roots |
Non-Patent Citations (3)
| Title |
|---|
| Pharmacokinetic comparisonsoftwodifferentcombinChemical taxonomy of tree peony species from China based on rootations of;Chunnian He等;《Phytochemistry》;20140911;69-79 * |
| 白芍多波长叠加指纹图谱研究;张慧等;《中药材》;20171108(第09期);2121-2124 * |
| 芍药总苷高效液相色谱指纹图谱研究;邹忠梅等;《药学学报》;20030128(第01期);46-49 * |
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