CN113584209A - Method for identifying wheat dominant variation chromosome and genetic effect thereof - Google Patents
Method for identifying wheat dominant variation chromosome and genetic effect thereof Download PDFInfo
- Publication number
- CN113584209A CN113584209A CN202110905349.XA CN202110905349A CN113584209A CN 113584209 A CN113584209 A CN 113584209A CN 202110905349 A CN202110905349 A CN 202110905349A CN 113584209 A CN113584209 A CN 113584209A
- Authority
- CN
- China
- Prior art keywords
- wheat
- chromosome
- variant
- chromosomes
- perinv6b
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000349 chromosome Anatomy 0.000 title claims abstract description 95
- 241000209140 Triticum Species 0.000 title claims abstract description 61
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 61
- 230000002068 genetic effect Effects 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000004458 analytical method Methods 0.000 claims abstract description 31
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 17
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract description 13
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract description 13
- 230000004069 differentiation Effects 0.000 claims abstract description 11
- 230000005540 biological transmission Effects 0.000 claims abstract description 5
- 238000005204 segregation Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 17
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 5
- 238000000546 chi-square test Methods 0.000 claims description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 2
- 102000003992 Peroxidases Human genes 0.000 claims description 2
- 102000001253 Protein Kinase Human genes 0.000 claims description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 2
- 239000004473 Threonine Substances 0.000 claims description 2
- 102000004139 alpha-Amylases Human genes 0.000 claims description 2
- 108090000637 alpha-Amylases Proteins 0.000 claims description 2
- 229940024171 alpha-amylase Drugs 0.000 claims description 2
- 238000007405 data analysis Methods 0.000 claims description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 2
- 108060006633 protein kinase Proteins 0.000 claims description 2
- 235000013339 cereals Nutrition 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000014509 gene expression Effects 0.000 abstract description 5
- 238000007901 in situ hybridization Methods 0.000 abstract description 4
- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 238000011160 research Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 7
- 238000009826 distribution Methods 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 238000003559 RNA-seq method Methods 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000003252 repetitive effect Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000000692 Student's t-test Methods 0.000 description 3
- 210000001726 chromosome structure Anatomy 0.000 description 3
- 230000002559 cytogenic effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012353 t test Methods 0.000 description 3
- COCMHKNAGZHBDZ-UHFFFAOYSA-N 4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(C([O-])=O)=CC=C1C(O)=O COCMHKNAGZHBDZ-UHFFFAOYSA-N 0.000 description 2
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- 108010034791 Heterochromatin Proteins 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 208000036878 aneuploidy Diseases 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004458 heterochromatin Anatomy 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000007479 molecular analysis Methods 0.000 description 2
- 230000004879 molecular function Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 2
- 101150090724 3 gene Proteins 0.000 description 1
- 102000013455 Amyloid beta-Peptides Human genes 0.000 description 1
- 108010090849 Amyloid beta-Peptides Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 238000000116 DAPI staining Methods 0.000 description 1
- 208000034423 Delivery Diseases 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000209124 Thinopyrum Species 0.000 description 1
- 241000234625 Thinopyrum bessarabicum Species 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 231100001075 aneuploidy Toxicity 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000024321 chromosome segregation Effects 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000003411 telomere Anatomy 0.000 description 1
- 108091035539 telomere Proteins 0.000 description 1
- 102000055501 telomere Human genes 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for identifying a wheat dominant variant chromosome and a genetic effect thereof, which integrates oligonucleotide probe fluorescence in-situ hybridization, wheat chip analysis, mixed segregation population phenotype comparison and BSR-seq analysis to reveal genetic transmission characteristics of the wheat variant chromosome and the effects on genome differentiation, gene expression and phenotype, provides a new scheme for exploring and utilizing the wheat dominant chromosome variant and key genes (clusters) thereof to improve wheat varieties, and belongs to the technical field of crop genetic breeding and agricultural biology.
Description
One, the technical field
The patent discloses a method for identifying dominant variant chromosomes and genetic effects of the dominant variant chromosomes of wheat, and belongs to the technical field of crop genetic breeding and agricultural biology.
Second, background Art
Common wheat is a typical allohexaploid species, whose Genome is significantly mutated in order to increase adaptability to stress during polyploidization, domestication and modification (see references: Badaeva E, Dedkova O, Gay G, Pukhalskyi V, Zelenin A, Bernard S, Bernard M. chromosoma reaangement in wheat: the hair types and distribution [ J ]. Genome, 2007, 50: 907-. With the rapid development of crop genomics, great progress was made in the analysis of genomic variation characteristics during wheat domestication and manual selection at the Molecular level, identifying a set of selectable marker sites and segments (see references: Hao C, Wang Y, Chao S, Li T, Liu H, Wang L, Zhang X. the iSelect 9K SNP analyzed transformed polyploidy mutation and intense human selection using transformed chromosome in wheat straw [ J ]. Scientific Reports, 2017, 7: 41; Hao C, Jiano C, Hou J, Li T, Liu H, Wang Y, Zhang J, Liu H, Bi Z, Xu F, Zhao J, Ma L, Y, joined U, Liyang X, applied R, sample R, Molecular analysis [ 1J ],247, Molecular analysis, 2020, 13: 1-19), which provides important reference for genome breeding based on omics, but the formation mechanism of the selectable marker and the segment thereof is still lack of deep knowledge, for example, the relationship between the chromosome variation and the evolution of wheat genome, and the specific effects of the chromosome variation on agronomic traits, adversity stress and gene expression regulation are only rarely and directly reported, wherein the great reason is that the current omics technology is difficult to sequence and assemble heterochromatin segments rich in highly repetitive sequences, so the identification of large-scale chromosome variation, especially reciprocal translocation, inversion, recessive aneuploidy and the like, is still dependent on cytogenetic analysis, but the early lack of simple and efficient chromosome identification technology makes the large-scale analysis of chromosome variation in the evolution of wheat varieties also have great difficulty; secondly, a technical system for integrating chromosome variation, plant genome evolution and genetic effect analysis into a system research is lacked, so that the current omics research and cytogenetic research are not combined with each other in many aspects.
Chromosomal variations reduce gene flow by either reducing fertility of the hybrids or by inhibiting recombination, thus causing reproductive segregation and the formation of new species (see reference: Schubert I. chromosome evolution [ J.)].Current opinion in plant biology,2007,10:109-115;Murat F,Xu J,Tannier E,Abrouk M,Guilhot N,Pont C,Messing J,Salse J.Ancestral grass karyotype reconstruction unravels new mechanisms of genome shuffling as a source of plant evolution[J]Genome research, 2010, 20: 1545-1557). The structural variation of chromosome has important influence on evolution and human disease occurrence due to the rearrangement of large-fragment DNA sequence, and is studied more deeply in animals, microorganisms and humans (de Joger R, Bolton M, Kombrink A, van den Berg G, Yadeta K, Thomma B. extended chromosome restriction driving evolution of viral in an anaerobic disease [ J.].Genome research,2013,23:1271-1282;Collins R,Brand H,Karczewski K,Zhao X,J,Francioli L,Khera A,Lowther C,Gauthier L,Wang H,Watts N,Solomonson M,O’Donnell A,Baumann A,Munshi R,Walker M,Whelan C,Huang Y,Brookings T,Sharpe T,Stone M,Valkanas E,Fu J,Tiao G,Laricchia K,Ruano V,Stevens C,Gupta N,Cusick C,Margolin L,Genome Aggregation Database Production T,Genome Aggregation Database C,Taylor K,Lin H,Rich S,Post W,Chen Y,Rotter J,Nusbaum C,Philippakis A,Lander E,Gabriel S,Neale B,Kathiresan S,Daly M,Banks E,MacArthur D,Talkowski M.A structural variation reference for medical and population genetics[J]Nature, 2020, 581: 444-451). However, the research on the plant is less, and particularly, the plant is more suitable for crops with huge genome, such as wheatThere is a lack of intensive research. The combination of genomics and cytogenetics promotes the research of chromosome variation mechanism and effect, and particularly, oligonucleotide probes developed based on genome sequencing provide powerful tools for clearly identifying large-range chromosome structure variation, and greatly promote the research progress of wheat chromosome structure and function. In this way, 8 oligonucleotide probe sets were successfully developed by the former research of the Nanjing university of agriculture for wheat (see references: Du P, Zhuang L, Wang Y, Yuan L, Wang Q, Wang D, Dawadondup, Tan L, Shen J, Xu H, ZHao H, Chu C, Qi Z. development of oligonucleotide and multiplex probes for nucleic and acid identification of wheat and tomato beta-amyloid [ J ] P]Genome, 2017, 60: 93-103), barley (see reference: zhang S, Zhu M, Shang Y, Wang J, Dawadundup, Zhuang L, Zhang J, Chu C, Qi z. physical organization of redundant sequences and chromosome diversity of barrel modified by Fluorescence In Situ Hybridization (FISH) [ J]Genome, 2019, 1: 329), maize (see references: zhu M, Du P, Zhuang L, Chu C, Qi Z.A simple and effective non-condensing FISH method for main chromosome diffusion using single-strand and oligonucleotide probes [ J]Genome, 2017, 60 (8): 657-: wu N, Li M, Sun H, Cao Z, Liu P, Ding T, Xu H, Chu C, Zhuang L, Qi Z.RNA-seq surfactants definition of chromosome-specific markers and transfer of eye chromosome to heat [ J]Molecular Breeding, 2018, 38: 6) thinopyrum bessarabicum (see reference: chen J, Tang Y, Yao L, Wu H, Tu X, Zhuang L, Qi Z. cytologic and molecular characterization of Thinopyrum bessaracinum chromogenes and structural rearrargements endogenous in steamed [ J]Molecular Breeding, 2019, 39: 146) and the chromosome identification of species, wherein the oligonucleotide probe set #4(ONPM #4) developed in wheat is utilized in the chromosome identification of wheat and related species thereof in a large amount due to simplicity and high efficiency, and becomes an important tool for identifying the chromosome variation of wheat (see reference: huang X, Zhu M, Zhuang L, Zhuang S, Wang J, Chen X, Wang D,Chen J,Bao Y,Guo J,Zhang J,Feng Y,Chu C,Du P,Qi Z,Wang H,Chen P.Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH[J]The therapeutic and Applied Genetics, 2018, 131: 1967-; wheat 'Chinese spring' aneuploid high-definition karyotype and use [ J ] based on oligonucleotide probe-sleeving padding]Crop academic newspaper, 2017, 43: 1575) and 1587) identifying a large number of structural variation and polymorphism chromosomes from Chinese wheat bred varieties and local varieties, wherein part of the variation chromosomes are derived from backbone parents and are transmitted frequently in derived varieties, and simultaneously identifying a large number of high-frequency polymorphism types which possibly represent dominant variation types of Chinese wheat, but an effective method is not available for identifying the genetic effect of the variation chromosomes, so that the dominant chromosome variation contained in wheat germplasm is difficult to determine and discover, and the application in breeding is limited.
Aiming at the problems, the invention provides a method for accurately identifying wheat mutation chromosomes by using oligonucleotide probe fluorescence in-situ hybridization, constructing a mixed separation population based on different mutation chromosome types by using a genetic population, identifying wheat dominant mutation chromosomes and revealing the effects of the mutation chromosomes on genome differentiation, phenotypic characters and gene expression regulation and control by using wheat chip hybridization, phenotypic identification and mixed pool transcriptome RNA-seq (BSR-seq) analysis, and providing a new scheme for researching and utilizing the wheat dominant mutation chromosomes and key genes (clusters) thereof to improve and evolve wheat varieties.
Third, the invention
The technical problem is as follows:
the invention aims to disclose a method for identifying dominant variant chromosomes and genetic effects of the dominant variant chromosomes.
The technical scheme is as follows:
1. in order to determine the type of wheat variant chromosome, oligonucleotide probe set FISH analysis is carried out on parents A and B based on the variant chromosome segregation population, and parents are compared to haveThe polymorphic or structural variation types of the differences are summarized as the signal distribution characteristics of the different segments of the paired variant chromosomes. Oligonucleotide probes using probe set ONPM # 4: repetitive sequences pSc119.2-1 and (GAA) comprising a 6-carboxyfluorescein (6-carboxyfluorescein, FAM, green) modification10And 6-carboxytetramethylrhodamine (6-carboxytetramethylrhodamine, TAMRA, Red) modified repeat sequences pAs1-1, pAs1-3, pAs1-4, pAs1-6, AFA-3 and AFA-4. All probe sequences are referenced to Du P, Zhuang L, Wang Y, Yuan L, Wang Q, Wang D, Dawadondup, Tan L, Shen J, Xu H, ZHao H, Chu C, Qi Z.development of oligonucleotides and multiplex probes for probes and primers identification of wheat and thinopum Bessarabacter chromogenes [ J.P. ]].Genome,2017,60:93-103。
2. In order to reveal the genetic transmission characteristics of wheat variant chromosome, firstly, a hybrid F of parent A/parent B related to the variant chromosome is constructed2To F2Carrying out ONMP # 4-based oligonucleotide probe set FISH analysis on the single plant to obtain a hybrid F of the variant chromosome2The distribution in the method is identified, the homozygous number and the heterozygous number of various variation types are analyzed, chi-square test is carried out on the variation types, and the genetic transmission characteristics are further analyzed. Secondly, chromosome composition analysis is carried out on the families in the high-generation line population of the Recombined Inbred Lines (RIL) with the same combination, and variant chromosomes related to different families are determined. The identified families were divided into different pairwise groups according to the type of chromosomal variation.
3. To investigate the population differentiation characteristics between different variant chromosome populations, the above identified population of parental A/parental B RIL high generation lines was genotyped in a wheat breeding chip, and the paired populations divided according to the wheat variant chromosome types were subjected to genetic differentiation index Analysis (Fst) (see references: Danecek P, Auton A, Absesas G, Albers C, Bank E, DePrist M, Handsaker R, Lunter G, Marth G, Sherry S, McVean G, Durbin R, genome Pronalysis G. the variant Analysis G. the variant call format and VCFtools [ J ]. Bioinformatics, 27: 2156-, 2020, 11: 5085) it is found that there are different SNP regions in the vicinity of the corresponding structural variation or polymorphism region between the paired clusters, and the size of the different region is determined. Genes within the interval were analyzed for their function in relation to molecular function, cellular composition, biological processes, etc. (see references: Tian T, Liu Y, Yan H, You Q, Yi X, Du Z, Xu W, Su Z. agriGO v 2.0: a GO analysis toolkit for the aggregative community, 2017 update [ J ]. Nucleic Acids Research, 2017, 45: 122-.
4. To analyze the effect of variant chromosomes on gene expression, BSR-Seq analysis was performed on the parental A/parental B RIL high generation population based on the type of chromosomal variation (see references: Wang Y, Xie J, Zhang H, Guo B, Ning S, Chen Y, Lu P, Wu Q, Li M, Zhang D, Guo G, Zhang Y, Liu D, Zou S, Tang J, Zhao H, Wang X, Li J, Yang W, Cao T, Yin G, Liu Z. mapping strain concrete yield gene YrZH22 in Chinee wot gene fusion Zhou ai 22 by bulk segregant RNA-Seq (BSR-Seq) and compatible genes analysis [ J. Applied, 130: 2011, SNP-related region-SNP-map-related region-SNP-related region-1, demarest B, Bisgrove B, Gorsi B, Su Y-C, Yost h. mmappr: tissue mapping analysis pipeline for porous RNA-seq [ J ]. Genome research, 2013, 23: 687-; ramirez R, Segovia V, Bird N, Fenwick P, Holdgate S, Berry S, Jack P, Camcamo M, Uauy C.RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for weaving in hexagonal leather [ J ]. Plant Biotechnology Journal, 2014, 13: 613-: FDR < 0.05, fold difference greater than 2 fold), which was annotated for function.
5. In order to reveal the effect of the variant chromosome on the wheat phenotype, a parent A/parent B RIL high generation line population is used for carrying out multi-year multi-point phenotype data investigation, the phenotype data of the family is divided into paired groups based on the chromosome variant type, and the IBM SPSS staticisics 22 is used for carrying out T-test analysis to clarify the influence of the variant chromosome on the phenotype.
Has the advantages that:
1. the ONMP # 4-based fluorescence in situ hybridization disclosed by the patent can clearly reveal wheat variant chromosomes, and provides important reference for sequencing and sequence assembly of centromere, telomere and other heterochromatin regions rich in highly-concatenated repetitive sequences;
2. the method for revealing the SNP difference interval between the variant chromosome groups by utilizing the wheat chip technology can quickly identify the differentiation of the variant chromosomes from the molecular level, is combined with the cytological result to assist in judging the break points of the variation, identifies the functional genes and metabolic pathways in the difference interval, and provides convenience for the research on the genome differentiation, the variety origin and evolution and the discovery of selective gene segments caused by the variation of the repetitive sequence;
3. the method for identifying the differential genes and phenotypes based on the mixed pool transcriptome sequencing of the wheat variant chromosomes provides a new scheme for discovering advantageous genes (clusters) related to dominant variant chromosomes and key sections thereof, and provides important information for the research of wheat origin and evolution, the variety improvement and the cultivation of backbone parents;
4. the method for identifying the dominant variant chromosome and the genetic effect of the dominant variant chromosome can be used for wheat natural populations and other plants, and provides an important reference for discovering the dominant variant chromosome and key trait genes of the plants.
Description of the drawings
FIG. 1: identification of Yangmai 158(Y158) and Fangshan wheat (FSXM) karyotypes and variant chromosomes thereof
The blue signal is DAPI staining; the green signal is FAM modified oligonucleotide probes pSc119.2-1 and (GAA)10(ii) a The red signal is TAMRA modified oligonucleotide probes pAs1-1, pAs1-3, pAs1-4, pAs1-6, AFA-3 and AFA-4. a: y158; b: FSXM; c: comparison of Y158 with FSXM karyotype; the red boxes represent polymorphic chromosomes present between parents; arrowFirst shown polymorphic chromosome segment
FIG. 2: Y158/FSXM F2Individual plant FISH with the same color as figure 1
FIG. 3: genetic differentiation index analysis based on wheat 15K chip chromosome polymorphism types PerInv6B and 6B-1
The position of the marker on the chromosome is marked by the horizontal coordinate, the unit is bp, and the Fst value is marked by the vertical coordinate
FIG. 4: genetic differentiation index analysis based on wheat 15K chip chromosome polymorphism types 5A-1 and 5A-4
The position of the marker on the chromosome is marked by the horizontal coordinate, the unit is bp, and the Fst value is marked by the vertical coordinate
FIG. 5: GO annotation of genes within 6B difference segments
FIG. 6: GO annotation of genes within 5A difference segments
FIG. 7: t-test analysis of trait between PerInv6B and class 6B clusters
Indicates significant difference, indicates very significant difference, and NS indicates insignificant difference; a: nanjing; b: linfen
FIG. 8: t-test analysis of traits between 4A-1 and 4A-3 clusters
Indicates significant difference, indicates very significant difference, and NS indicates insignificant difference; a: nanjing; b: linfen
FIG. 9: trait T-test analysis between 5A-1 and 5A-4 clusters
Indicates significant difference, indicates very significant difference, and NS indicates insignificant difference; a: nanjing; b: linfen
FIG. 10: t-test analysis of traits between 4B-1 and 4B-2 groups
Indicates significant difference, indicates very significant difference, and NS indicates insignificant difference; a: nanjing; b: linfen
Fifth, detailed description of the invention
1. Identification of variant chromosomes in Yangmai 158 and Fangshan wheat
The oligonucleotide probe set developed by Du et al.2017 (see references Du P, Zhuang L, Wang Y, Yuan L, Wang Q, Wang D, Dawadondup, Tan L, Shen J, Xu H, Zhuao H, Chu C, Qi Z.development of oligonucleotides and multiplex probes for purposes and acquisition identification of wheat and thin beer Bessarabics chromosomes [ J ]. Genome, 2017, 60: 93-103) was used for the analysis of wheat varieties modified by Huang J, Cheng X, Wang D, Chen J, Bao Y, Guo J, Huang Y, Wang J, Huang H, Zhu H, Chu J # 1, Huang J, Huang D, Cheng J, wheat varieties of wheat, wheat varieties # modified by Huang H, Shu J, Huang J, Qian H, Huang H, and Huang H # 2, and chromosome # 2, wheat varieties, wheat. According to the nomenclature of polymorphic chromosomes in Huang et al (2018), Y158-4A, Y158-5A, Y158-4B, Y158-6B, Y158-7B corresponds to 4A-3, 5A-4, 4B-2, perInv6B and 7B-1 respectively; FSXM-4A, FSXM-5A, FSXM-4B, FSXM-6B, FSXM-7B corresponds to 4A-1, 5A-1, 4B-1, 6B-1 and 7B-7 respectively. Five pairs of chromosomes are found to have difference between two parents, and 1 chromosome structure variation and 5 pairs of polymorphic chromosomes are involved, and are respectively as follows: 4A-3 vs 4A-1, 5A-4 vs 5A-1, 4B-2 vs 4B-1, PerInv6B vs 6B-1, 7B-1 vs 7B-7 (FIG. 1). 4A-1, the end of the long arm has more green signals than 4A-3; 5A-4 has more than 5A-1 long arm middle part a pair of weak green signals, 5A-1 red signal is obviously stronger than 5A-4; 4B-1 and 4B-2 differ in the presence or absence of a green spot at the proximal end of the short arm, 4B-1 is absent, and 4B-2 is present; 6B differ by the presence or absence of PerInv 6B; the end of the long arm of 7B-1 has a significantly stronger green signal than that of 7B-7.
2. Genetic transmission of variant chromosomes
To understand the polymorphic chromosomes 4A, 5A, 4B, 6B, 7B at Y158/FSXM hybrid F2Distribution in (1) for 288F strains2Identification was performed (FIG. 2) and a total of 145 karyotypes were found, wherein the number of Y158 type homozygoses, the number of heterozygous type homozygotes, and the number of FSXM type homozygotes are shown in Table 1. Proved by chi-square test, the ratio of the Y158-4A homozygous type, the Y heterozygous type and the FSXM-4A homozygous type is obviously different from the theoretical separation ratio of 1: 2: 1 (df is 2, chi)2More than 5.99, P is less than 0.05); the Y158-perInv6B homozygous type, parental mixed type, and FSXM-6B homozygous type ratios were significantly different from the theoretical separation ratio of 1: 2: 1 (df 2, χ)2More than 9.21, P is less than 0.01); the remaining polymorphic chromosomes and theoryNo significant difference in separation ratios (df 2, χ)2< 5.99, P > 0.05), indicating normal delivery.
For Y158/FSXM RIL-F8The identification of 62 families showed that: only the distribution of chromosomes perInv6B and 6B-1 is biased apart (df is 1, χ)2> 3.84, P < 0.05) (Table 1), other chromosomes were isolated normally.
For RIL F8124 seeds of the self-bred generation of the hybrid PerInv6B individual plant are identified, and the homozygous type, heterozygous type and 6B-1 homozygous type of the PerInv6B are 31: 67: 26, which are consistent with 1: 2: 1.
TABLE 1 polymorphic chromosomes in Yang158/Fangshan wheat F2And F8Distribution in
3. Effect of variant chromosomes on population differentiation
For the above identified Y158/FSXM RIL-F8Genotype analysis of wheat 15K chip is carried out, and genetic differentiation index analysis (Fst) is carried out on five pairs of clusters divided according to wheat variant chromosome types, so that difference SNP intervals exist near corresponding inversion or polymorphism segments between two pairs of clusters of PerInv6B vs 6B-1 (figure 3) and 5A-4 vs 5A-1 (figure 4), and the difference segments respectively
TABLE 2 polymorphic chromosomal Difference segment information
367.05Mb, 51.97Mb (Table 2), the sites of variation among other variant chromosome clusters are less or need to be confirmed further with a higher density of markers. The gene annotation shows that the genes in the difference interval of PerInv6B vs 6B-1 are significantly enriched in 10 functions, mainly related to cell development, cell surface receptor signal transduction pathways and the like (figure 5); the genes in the 5A-4 vs 5A-1 difference interval are significantly enriched in molecular functions such as beta-glucosidase activity, acylglycerol O-acyltransferase activity, etc. (FIG. 6).
4. Effect of variant chromosomes on Gene expression
For Y158/FSXM RIL-F10BSR-seq analysis based on chromosome variation types revealed that SNP differences exist in five regions of the 6B chromosome between PerInv6B and 6B-1 pool, and the SNP differences comprise 801 genes in total, wherein 21 genes are significantly differentially expressed between parents and pool at the same time, and alpha-amylase, serine, threonine protein kinase, peroxidase and the like are involved (Table 3).
TABLE 3 Gene annotation of differentially genes in regions of significant 6B differences between pools
5. Influence of variant chromosomes on phenotype
Using Y158/FSXM RIL-F10Two-point three-repeat data analysis of differences between five pairs of variant groups shows that the effects of different variant chromosomes exist in different environments, wherein perInv6B has the effects of reducing plant height in Nanjing, reducing effective spikelet number and panicle number in two places in Nanjing Zheng (FIG. 7), 4A-3 has the effects of reducing plant height, reducing tillering, reducing panicle length and reducing panicle number in Zheng (FIG. 8), 5A-4 has the effects of reducing plant height and shortening panicle length in Zheng (FIG. 9), 4B-2 has the effects of reducing plant height, increasing panicle number and reducing panicle length in Nanjing (FIG. 10), and the obvious phenotypic effect of the variant chromosomes is confirmed, but is influenced by various factors, is not completely consistent in two places, and further repetition is needed.
The above embodiments are merely exemplary embodiments adopted to illustrate the system disclosed in the patent, however, the present invention is not limited thereto, and those skilled in the art can make various modifications and changes without departing from the spirit of the present invention, and these modifications and changes also fall into the scope of the present invention.
Claims (3)
1. A method for identifying wheat dominant variant chromosome and genetic effect thereof comprises the following steps:
(1) the method is characterized in that an oligonucleotide probe set ONMP #4 is used for identifying variant chromosomes (including structural variant chromosomes and polymorphic chromosomes) in Yangmai 158(Y158) and Fangshan wheat (FSXM), and five pairs of chromosomes are found to have differences, namely: 4A-3 vs 4A-1, 5A-4 vs 5A-1, 4B-2 vs 4B-1, perInv6B vs 6B-1, 7B-1 vs 7B-7;
(2) for the constructed Yangmai 158/mountain-turning wheat hybrid F2Population, Recombinant Inbred Line (RIL) F8Generation group and RIL-F8The chromosome composition analysis of the inbred population of the heterozygous individual revealed the genetic transmission of the variant chromosomes identified in (1), and the chi-square test was performed, except that perInv6B was partially separated in both populations and 4A-3 was performed at F2In addition to segregation in the population, the remaining variant chromosomes are normally segregated, but in RIL-F8The heterozygous individual plants are bred into a progeny group, and the PerInv6B is normally separated;
(3) the Y158/FSXM RIL-F in (2)8Carrying out genotype analysis on a wheat 15K chip, and carrying out genetic differentiation index analysis (Fst) on five pairs of clusters divided according to the types of wheat variant chromosomes to find that difference SNP (single nucleotide polymorphism) intervals exist between two pairs of clusters of PerInv6B vs 6B-1 and 5A-4 vs 5A-1 (figure 4) at the corresponding inversed positions or near polymorphic segments, wherein the difference segments are 367.05Mb and 51.97Mb respectively;
(4) for Y158/FSXM RIL-F10Dividing the chromosome into two mixed separation groups based on chromosome variation types, and finding that 21 genes are differentially expressed between parent and PerInv6B and 6B-1 mixed pools and relate to genes such as alpha-amylase, serine, threonine protein kinase, peroxidase and the like;
(5) using Y158/FSXM RIL-F10Two-point and three-repetition phenotypic data analysis shows that PerInv6B, 5A-4 and 3B-2 respectively have the effects of reducing plant height, increasing grain number per spike, increasing grain length per spike and the like under specific environments.
2. Use of the method of claim 1 in the identification of dominant variant chromosomes in wheat and key genes (clusters) thereof.
3. Use of the method of claim 1 in wheat breeding.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110905349.XA CN113584209A (en) | 2021-08-06 | 2021-08-06 | Method for identifying wheat dominant variation chromosome and genetic effect thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110905349.XA CN113584209A (en) | 2021-08-06 | 2021-08-06 | Method for identifying wheat dominant variation chromosome and genetic effect thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113584209A true CN113584209A (en) | 2021-11-02 |
Family
ID=78256122
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110905349.XA Pending CN113584209A (en) | 2021-08-06 | 2021-08-06 | Method for identifying wheat dominant variation chromosome and genetic effect thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113584209A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101792797A (en) * | 2009-11-24 | 2010-08-04 | 南京农业大学 | Method for breeding common wheat-thinopyrum bessarabicum small-fragment translocation line and molecular marker thereof |
CN112273221A (en) * | 2020-11-05 | 2021-01-29 | 南京农业大学 | Breeding method of high-hardness wheat |
-
2021
- 2021-08-06 CN CN202110905349.XA patent/CN113584209A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101792797A (en) * | 2009-11-24 | 2010-08-04 | 南京农业大学 | Method for breeding common wheat-thinopyrum bessarabicum small-fragment translocation line and molecular marker thereof |
CN112273221A (en) * | 2020-11-05 | 2021-01-29 | 南京农业大学 | Breeding method of high-hardness wheat |
Non-Patent Citations (5)
Title |
---|
XINYI HUANG等: "Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high-resolution multiplex oligonucleotide FISH", 《THEOR APPL GENET》, vol. 131, no. 9, 15 June 2018 (2018-06-15), pages 2 - 3, XP036567725, DOI: 10.1007/s00122-018-3126-2 * |
张瑞奇等: "普通小麦-簇毛麦T5VS?5DL易位染色体对小麦主要农艺性状、品质和白粉病抗性的遗传效应分析", 《中国农业科学》, no. 06, pages 1041 - 1051 * |
李亚莉等: "八倍体小偃麦和硬粒小麦杂交后代的染色体组成分析", 《中国种业》, no. 4, 25 March 2019 (2019-03-25), pages 51 - 55 * |
王丹蕊等: "基于寡核苷酸探针套painting的小麦"中国春"非整倍体高清核型及应用", 《作物学报》, no. 11, pages 1575 - 1587 * |
马超等: "高产小麦新品种百农207及其姊妹系的基因组构成分析", 《郑州大学学报(理学版)》, vol. 52, no. 2, 27 December 2019 (2019-12-27), pages 118 - 126 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Fulnecek et al. | Evolution and structure of 5S rDNA loci in allotetraploid Nicotiana tabacum and its putative parental species | |
Burr et al. | Gene mapping with recombinant inbreds in maize. | |
AU2013205620B2 (en) | Cotton event pDAB4468.19.10.3 detection method | |
RU2577143C2 (en) | Detection of aad-1 of object das-40278-9 | |
CN105256031B (en) | Utilize the method and its primer special of high-throughput molecular labeling transformation muskmelon female series | |
US8710295B2 (en) | Soybean sequences associated with the FAP3 locus | |
Wu et al. | Predominant wheat-alien chromosome translocations in newly developed wheat of China | |
Li et al. | Molecular dissection of Secale africanum chromosome 6R afr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust | |
Riday et al. | Genetic map-based location of the red clover (Trifolium pratense L.) gametophytic self-incompatibility locus | |
Martin et al. | A rapid method to map mutations in Drosophila | |
CN108611428A (en) | Genetic marker and application of the TGFB3 gene pleiomorphisms as pig body length and number of nipples | |
CN113699268B (en) | Wheat thousand grain weight character related SNP site and application thereof | |
Zhang et al. | Fine mapping and distribution analysis of hybrid necrosis genes Ne1 and Ne2 in wheat in China | |
JP2008526253A (en) | DNA markers for increased milk production in cattle | |
WO2023232152A1 (en) | Multiplex kasp marker primer set for wheat plant height major genes and use thereof | |
CN111334597B (en) | SNP (Single nucleotide polymorphism) site and KASP (Kaempferi protein) marker for detecting powdery mildew resistance of watermelon and application thereof | |
CN116064583B (en) | Highland barley spike length regulating gene and KASP molecular marker and application thereof | |
CN113584209A (en) | Method for identifying wheat dominant variation chromosome and genetic effect thereof | |
CN107447022B (en) | SNP molecular marker for predicting corn heterosis and application thereof | |
CN114480709B (en) | Molecular marker for detecting wheat leaf rust resistance gene Lr47, detection method and application thereof | |
CN115976270A (en) | Molecular marker related to regulation and control of early blossoming and dwarf of corn and application of molecular marker | |
King et al. | Introgression mapping in the grasses | |
CN114317807A (en) | Wheat thousand grain weight character related SNP site and application thereof | |
Zhang et al. | KASP markers to detect sub-chromosomal arm translocations between 6VS of Haynaldia villosa and 6AS of wheat | |
CN117887885B (en) | Soybean oil content-related major single nucleotide polymorphism site and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211102 |
|
RJ01 | Rejection of invention patent application after publication |